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1.
Food Chem ; 324: 126863, 2020 Sep 15.
Artigo em Inglês | MEDLINE | ID: mdl-32353657

RESUMO

In this study, 95 accessions of proso millet (Panicum miliaceum L.) were characterized for starch physicochemical properties, including apparent amylose content (AAC), gel textural properties, Rapid Visco Analyzer (RVA) pasting viscosity properties, thermal and retrogradation properties. Based on genotypic data, the genetic diversity and inter-relationship of these starch traits were analyzed. Diverse starch quality was found, for example, AAC ranged from 0 to 32.3%, gelatinization temperature (GT) varied from 71.5 to 79.0 ℃, and RVA profile showed distinct patterns among proso millet of different AAC types. Interestingly, high AAC proso millet usually had GT lower than that of low AAC proso millet, which is different from the findings in rice starch. Many starch traits were significantly correlated and most of the 18 tested traits could be classified as either AAC-related traits or GT-related traits. In summary, the information presented here will be useful for further development of proso millet products.

2.
Molecules ; 25(5)2020 Mar 01.
Artigo em Inglês | MEDLINE | ID: mdl-32121474

RESUMO

Sensitive and selective detection of harmful gas is an important task in environmental monitoring. In this work, a gas sensor based on cataluminescence (CTL) for detection of acetaldehyde was designed by using nano-NiO as the sensing material. The sensor shows sensitive response to acetaldehyde at a relatively low working temperature of 200 °C. The linear range of CTL intensity versus acetaldehyde concentration is 0.02-2.5 mg/L, with a limit of detection of 0.006 mg/L at a signal-to-noise ratio of three. Mechanism study shows that electronically excited CO2 is the excited intermediate for CTL emission during the catalytic oxidation of acetaldehyde on the NiO surface. The proposed sensor has promising application in monitoring acetaldehyde in residential buildings and in the workplace.

3.
Neuropharmacology ; 170: 108041, 2020 Jun 15.
Artigo em Inglês | MEDLINE | ID: mdl-32213357

RESUMO

Our previous studies have shown that ginsenoside Rg1 (Rg1) exerts antidepressant-like effects in animal models of depression, accompanied by an improvement of astrocytic gap junction functions. However, whether connexin 43 (Cx43), the major connexin forming gap junctions between astrocytes, is the key regulator of Rg1-induced antidepressant-like effects is still unknown. In this study, we examine in vitro and in vivo the involvement of Cx43 in the antidepressant effects of Rg1. Corticosterone was used to establish an in vitro rat model of depression. Treatment with Rg1 1 h prior to corticosterone significantly improved the cell viability of astrocytes, which was significantly inhibited by carbenoxolone, a widely used gap junction inhibitor. Moreover, Rg1 treatment significantly ameliorated antidepressant-sensitive behaviours induced by infusion of carbenoxolone or Gap26, a selective inhibitor of Cx43, into the prefrontal cortex of the animals. Rg1 treatment increased the expression of Cx43 compared with Gap26 group. According to these results, the antidepressant-like effects of Rg1 were mainly mediated by Cx43-formed gap junctions.

4.
Analyst ; 145(8): 3100-3105, 2020 Apr 14.
Artigo em Inglês | MEDLINE | ID: mdl-32150184

RESUMO

A coumarin-diaminomaleonitrile derivative (C-DAN) was designed and prepared. Upon addition of hypochlorite (OCl-), the fluorescence emission peak of C-DAN at 475 nm was gradually enhanced with a rapid response (within 5 s); meanwhile, the color of the solution changed from dark red to bright yellow which can be observed with the naked eye. Furthermore, owing to its advantages including high selectivity, sensitivity, and low toxicity, our C-DAN probe has been applied for detecting OCl- in living cells and zebrafish, indicating it is an ideal tool for biological applications.

5.
Sichuan Da Xue Xue Bao Yi Xue Ban ; 51(2): 252-256, 2020 Mar.
Artigo em Chinês | MEDLINE | ID: mdl-32220196

RESUMO

Objective: To analyze the phenotype and genotype in two pedigrees with hereditary coagulation factor Ⅺ (FⅪ) deficiency, and investigate the molecular mechanisms of FⅪ deficiency. Methods: Two patients with hereditary coagulation FⅪ deficiency were admitted to Chaozhou Central Hospital in Nov 2014 and Jan 2018. The prothrombin time (PT), activated partial thromboplastin time (APTT), FⅪ activity (FⅪ∶C) and FⅪ antigen (FⅪ∶Ag) were tested for phenotypic diagnosis. All the exons and exon-intron boundaries of FⅪ gene of proband were analyzed by PCR and sequencing. The family members were tested for the mutant site of proband. Then the mRNA of FⅪ in the proband was analyzed with RT-PCR. Results: The proband-1 was a 7-year-old boy, PT was 10.7 s and APTT was 97.4 s (reference range: 9-12.8 s; 24-40 s), FⅪ∶C (0.6%) and FⅪ∶Ag<1% (reference range: 65%-150%; 72.1%-122.3%). The proband-2 was a 30-year-old female, and showed the PT (11.7 s), APTT (71.3 s), FⅪ∶C (0.7%) and FⅪ∶Ag<1%. FⅧ∶C, FⅨ∶C and FⅫ∶C of two proband were within the normal range. DNA sequencing showed that the proband-1 had a combined mutation of c.326-1G>A and c.1107C>A (p.Tyr351X) in exon 10. His grandmother, mother and brother had a heterozygous splicing mutation of c.326-1G>A, his grandmother and father had a homozygous mutation of c.1107C>A. FXI mRNA was undetected in the proband-1. The proband-2 had a homozygous mutation of c.841C>T (p.Gln263X) in exon 8, and this mutation was also found in her father, mother, daughter and son. Conclusion: The c.326-1G>A, c.1107C>A(p.Tyr351X) and c.841C>T (p.Gln263X) might be the molecular pathogenesis for two probands with hereditary coagulation factor Ⅺ deficiency.


Assuntos
Deficiência do Fator XI , Fator XI , Linhagem , Fenótipo , Adulto , Criança , Fator XI/genética , Deficiência do Fator XI/genética , Feminino , Genótipo , Heterozigoto , Humanos , Masculino , Mutação , Reação em Cadeia da Polimerase , Análise de Sequência de DNA
6.
Int J Biol Macromol ; 149: 717-723, 2020 Apr 15.
Artigo em Inglês | MEDLINE | ID: mdl-32014483

RESUMO

Although immunomodulatory activities of Dendrobium officinale polysaccharide has been investigated for many years, yet the potential contribution of its metabolite derived from intestinal microbes on immunoregulation effect has not been reported. In this study, polysaccharide DOW-5B with average molecular weight of 39.4 kDa was isolated from the stem of Dendrobium officinale Kimura et Migo. The carbohydrate content was 91.97% and no protein was detected. The monosaccharide analysis showed this polysaccharide was composed of glucuronic acid and glucose at a molar ratio (M/G) of 1.2:19.4. Animal test indicated DOW-5B increased the diversity of gut microbiota on mice. Beneficial microbes such as Ruminococcus, Eubacterium, Clostridium, Bifidobacterium, Parabacteroides and Akkermansiamuciniphila increased while harmful bacteria in Proteobacteria decreased. Surprisingly, DOW-5B promoted gut microbes to generate more butyrate and mainly produced by Parabacteroides_sp_HGS0025. Further, we found the health of large intestine as well as immunity response of mice was improved. In addition, Parabacteroides_sp_HGS0025 positively correlated with butyrate, IgM, IL-10, and TNF-α products in intestine and mice blood, respectively. The data suggested that Dendrobium officinale polysaccharide has function on immunity may be mediated by butyrate. It adds new evidence to support the basis of how herbal polysaccharides affect immunity.

7.
Food Chem ; 316: 126263, 2020 Jun 30.
Artigo em Inglês | MEDLINE | ID: mdl-32045812

RESUMO

In this study, starch was isolated from 13 genotypes of 12 Lycoris species, and the morphology, granule size distribution and physicochemical properties, including apparent amylose content (AAC), Rapid Visco Analyzer (RVA) pasting properties, textural properties, thermal and retrogradation properties were characterized. The majority of starch granules of the 13 Lycoris genotypes were oval in shape, and granule size followed a normal distribution with a mean diameter of 20-30 µm. Contrary to previously published findings, the XRD results revealed that lycoris starches had either C-type or CA-type crystallinity. All lycoris starches showed high AAC varying from 25.6% to 32.7%, and low gelatinization temperature (GT) ranging from 58.8 to 69.7℃. Inter-relationships among 18 starch quality traits were analyzed based on correlation analysis. The present study provides information on lycoris starch characteristics which should serve as a useful guide for later studies on lycoris starch utilization in food and non-food industries.

8.
Aging (Albany NY) ; 12(4): 3205-3217, 2020 Feb 21.
Artigo em Inglês | MEDLINE | ID: mdl-32087604

RESUMO

Long non-coding RNAs (lncRNAs) play important roles in the progression of cervical cancer (CC). However, the roles and underlying molecular mechanisms of lncRNAs in CC remain unclear. In the current study, we discovered a new lncRNA MIR210HG which was upregulated in CC tissues through microarray. The upregulation of MIR210HG was associated with advanced FIGO stage, metastasis, and poor prognosis in CC patients. Function assays showed that MIR210HG inhibition significantly suppressed the proliferation, invasion, and epithelial-mesenchymal transition (EMT) processes in CC and reduced tumor growth in vivo. Mechanistically, we identified that MIR210HG might serve as a competing endogenous RNA (ceRNA) of miR-503-5p to relieve the repressive effect of miR-503-5p on TRAF4 expression in CC cells. In conclusion, we demonstrated that MIR210HG promoted CC progression through regulating the MIR210HG/miR-503-5p/TRAF4 axis, indicating that MIR210HG might act as a novel insight into CC treatment.

9.
FEBS Open Bio ; 2020 Jan 03.
Artigo em Inglês | MEDLINE | ID: mdl-31898405

RESUMO

Pancreatic ductal adenocarcinoma (PDAC) is one of the most aggressive solid tumors in the digestive system. A greater understanding of the pathogenesis of PDAC may facilitate the search for new therapeutic targets. Guanine nucleotide-binding protein subunit gamma-12 (GNG12) belongs to the G protein family and participates in the modulation of the inflammatory signaling cascade. However, the cancer-related function and clinical relevance of GNG12 in PDAC have not previously been reported. Here, we investigated the clinical significance of GNG12 in PDAC using the Oncomine web tool, the gene expression profiling interactive analysis tool and tissue microarray (TMA). GNG12 expression was observed to be higher in PDAC patient specimens than in nontumor pancreatic tissues, and high expression of GNG12 was associated with poor prognosis. We subsequently show that GNG12 promotes pancreatic cancer cell growth in vivo and in vitro, as evaluated using 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium, inner salt assays, colony formation assays and a xenograft mouse model. Furthermore, our results suggest that GNG12 activates nuclear factor-κB signaling and modulates the immune response. Collectively, our findings suggest that GNG12 may be suitable as a new prognosis-related biomarker and a promising target for treatment of pancreatic cancer.

10.
Environ Sci Technol ; 54(3): 1909-1919, 2020 02 04.
Artigo em Inglês | MEDLINE | ID: mdl-31886657

RESUMO

In this study, a permanganate/redox mediator system for enhanced transformation of a series of emerging contaminants was evaluated. The presence of various redox mediators (i.e., 1-hydroxybenzotriazole, N-hydroxyphthalimide, violuric acid, syringaldehyde, vanillin, 4-hydroxycoumarin, and p-coumaric acid) accelerated the degradation of bisphenol A (BPA) by Mn(VII). Since 1-hydroxybenzotriazole (HBT) exhibited the highest reactive ability, it was selected to further investigate the reaction mechanisms and quantify the effects of important reaction parameters on Mn(VII)/redox-mediator reactions with BPA and bisphenol AF (BPAF). Interestingly, not only HBT accelerated the degradation of BPA, but also BPA enhanced the decay of HBT. Evidence for the in situ formation of HBT· radicals as the active oxidant responsible for accelerated BPA and BPAF degradation was obtained by radical scavenging experiments and 31P NMR spin trapping techniques. The routes for HBT· radical formation involving Mn(VII) and the electron-transfer pathway from BPA/BPAF to HBT· radicals demonstrate that the Mn(VII)/HBT system was driven by the electron-transfer mechanism. Compared to Mn(VII) alone, the presence of HBT totally inhibited self-coupling of BPA and BPAF and promoted ß-scission, hydroxylation, ring opening, and decarboxylation reactions. Moreover, Mn(VII)/HBT is also effective in real waters with the order of river water > wastewater treatment plant (WWTP) effluent > deionized water.


Assuntos
Compostos Benzidrílicos , Compostos de Manganês , Oxidantes , Oxirredução , Óxidos
11.
BMC Med ; 17(1): 204, 2019 11 15.
Artigo em Inglês | MEDLINE | ID: mdl-31727112

RESUMO

BACKGROUND: Brain innate immunity is vital for maintaining normal brain functions. Immune homeostatic imbalances play pivotal roles in the pathogenesis of neurological diseases including Parkinson's disease (PD). However, the molecular and cellular mechanisms underlying the regulation of brain innate immunity and their significance in PD pathogenesis are still largely unknown. METHODS: Cre-inducible diphtheria toxin receptor (iDTR) and diphtheria toxin-mediated cell ablation was performed to investigate the impact of neuron-glial antigen 2 (NG2) glia on the brain innate immunity. RNA sequencing analysis was carried out to identify differentially expressed genes in mouse brain with ablated NG2 glia and lipopolysaccharide (LPS) challenge. Neurotoxin 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP)-treated mice were used to evaluate neuroinflammatory response in the presence or absence of NG2 glia. The survival of dopaminergic neurons or glial cell activation was evaluated by immunohistochemistry. Co-cultures of NG2 glia and microglia were used to examine the influence of NG2 glia to microglial activation. RESULTS: We show that NG2 glia are required for the maintenance of immune homeostasis in the brain via transforming growth factor-ß2 (TGF-ß2)-TGF-ß type II receptor (TGFBR2)-CX3C chemokine receptor 1 (CX3CR1) signaling, which suppresses the activation of microglia. We demonstrate that mice with ablated NG2 glia display a profound downregulation of the expression of microglia-specific signature genes and remarkable inflammatory response in the brain following exposure to endotoxin lipopolysaccharides. Gain- or loss-of-function studies show that NG2 glia-derived TGF-ß2 and its receptor TGFBR2 in microglia are key regulators of the CX3CR1-modulated immune response. Furthermore, deficiency of NG2 glia contributes to neuroinflammation and nigral dopaminergic neuron loss in MPTP-induced mouse PD model. CONCLUSIONS: These findings suggest that NG2 glia play a critical role in modulation of neuroinflammation and provide a compelling rationale for the development of new therapeutics for neurological disorders.

12.
Int J Biol Macromol ; 2019 Nov 16.
Artigo em Inglês | MEDLINE | ID: mdl-31743712

RESUMO

In this study, a novel matrix protein (teosin) was isolated from Hyriopsis cumingii. Gene expression analysis showed that teosin is mainly expressed in the mantle and blood, and a hybridization signal was found in dorsal epithelial cells of the mantle pallial by in situ hybridization. Moreover, teosin expression during pearl formation indicated its participation in initial nacreous layer biomineralization, and suppressing teosin expression resulted in irregular crystal morphology and disordered arrangement in RNAi assay. In vitro crystallization assays indicated teosin could increase the size of calcite. By turning the sample stage about 15°, we got the high-resolution TEM images of the crystals' edges. This is a novel method to observe the crystal which is over 200 nm under TEM. In the control experiment group, the calcite show the character of long range order. The calcite induced by teosin were composed of nano-grains, and the polycrystal character were confirmed by EDS. These results suggested that teosin is involved in regulating crystal morphology regulation and inducing polycrystal formation during nacreous-layer formation.

13.
Front Psychol ; 10: 2023, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31543856

RESUMO

This study aims to examine the relationship among the Dark Triad personality traits, Internet use preferences and Internet adaptation. In all, 1927 middle school students (aged 11-18) from Beijing and Kunming in China completed a self-reported questionnaire that measured the Dark Triad, Internet use preferences (information communication, recreation, information acquisition and online transaction) and Internet adaptation (positive/negative Internet adaptation). Correlations revealed that there were significant correlations among the Dark Triad, Internet use preferences and Internet adaptation. Multiple regression analysis indicated that psychopathy predicted Internet adaptation more significantly than Machiavellianism or narcissism. Information acquisition positively predicted positive Internet adaptation and negatively predicted negative Internet adaptation, and recreation and online transaction were just the opposite. The SEM results suggested that Internet use preferences partially mediated the relationship between the Dark Triad and Internet adaptation. We discussed the importance of the combined effect of the Dark Triad and Internet use preferences on Internet adaptation.

14.
Chem Asian J ; 14(19): 3344-3349, 2019 Oct 01.
Artigo em Inglês | MEDLINE | ID: mdl-31432590

RESUMO

A novel visible-light-driven decarboxylative coupling of alkyl N-hydroxyphthalimide esters (NHP esters) with quinoxalin-2(1H)-ones has been developed. This C(sp2 )-C(sp3 ) bond-forming transformation exhibits excellent substrate generality with respect to both the coupling partners. Of note, a series of 3-primary alkyl-substituted quinoxalin-2(1H)-ones that were difficult to synthesize by previous methods could be obtained in moderate to excellent yields. Additionally, the mild conditions, easy availability of substrates, wide functional group tolerance and operational simplicity make this protocol practical in the synthesis of 3-alkylated quinoxalin-2(1H)-ones.

15.
Opt Lett ; 44(13): 3354-3357, 2019 Jul 01.
Artigo em Inglês | MEDLINE | ID: mdl-31259959

RESUMO

We propose a thin-film InSb-based thermoradiative system (TRS) and assess its performance characteristics by using a parametric design at low-grade waste heat. We consider the effects of several loss mechanisms on system performance, including optical, sub-gap radiation, and non-radiative losses. Our results predict that the 50 nm thick InSb TRS operating with a hot (cold) source at 500 K (300 K) may yield a power density of 113 Wm-2 and an efficiency limit of 10.5%. To enhance the system performance, more efforts should be paid to optimize the layer thickness, enhance optical radiation, improve surface passivation, and fabricate an Ag back-reflective mirror and an optical filter for frequency-dependent photon recycling. This Letter provides new insights, to the best of our knowledge, for optimal designs and energy loss mechanisms, thus paving a route towards the development of practical TRS at a low temperature of around 500 K.

16.
Nat Commun ; 10(1): 2386, 2019 06 03.
Artigo em Inglês | MEDLINE | ID: mdl-31160591

RESUMO

The fast development of single-particle cryogenic electron microscopy (cryo-EM) has made it more feasible to obtain the 3D structure of well-behaved macromolecules with a molecular weight higher than 300 kDa at ~3 Å resolution. However, it remains a challenge to obtain the high-resolution structures of molecules smaller than 200 kDa using single-particle cryo-EM. In this work, we apply the Cs-corrector-VPP-coupled cryo-EM to study the 52 kDa streptavidin (SA) protein supported on a thin layer of graphene and embedded in vitreous ice. We are able to solve both the apo-SA and biotin-bound SA structures at near-atomic resolution using single-particle cryo-EM. We demonstrate that the method has the potential to determine the structures of molecules as small as 39 kDa.


Assuntos
Biotina/metabolismo , Microscopia Crioeletrônica/métodos , Imagem Individual de Molécula/métodos , Estreptavidina/ultraestrutura , Grafite , Substâncias Macromoleculares/ultraestrutura , Modelos Moleculares , Conformação Molecular , Estreptavidina/metabolismo
17.
Int J Biol Macromol ; 135: 745-751, 2019 Aug 15.
Artigo em Inglês | MEDLINE | ID: mdl-31152837

RESUMO

Shell biomineralization is a process where inorganic minerals accumulate upon a chitinous scaffold under the control of multifunctional matrix proteins. In this study, we cloned a novel matrix protein gene from the mantle of Hyriopsis cumingii. The predicted protein, hichin, contains a chitin-binding domain and exhibited the highest expressional level in mantle tissue, with positive signals mainly detected in dorsal epithelial cells of the pallial mantle according to in situ hybridization, indicating its possible involvement in shell nacreous layer biomineralization. RNA interference showed that hichin suppression induced disordered self-assembly of the insoluble framework in the nacreous layer, and that the newly formed calcium carbonate crystals could not bind to organic frameworks. Furthermore, hichin was primarily responsible for building the framework during initial nacre deposition in pearl formation. Moreover, the chitin-binding domain of hichin also provided crystal morphology regulation in vitro crystallization assay. These results indicated that hichin is involved in the self-assembly of organic frameworks and morphological regulation in shell nacreous layer.


Assuntos
Exoesqueleto/crescimento & desenvolvimento , Carbonato de Cálcio/metabolismo , Quitina/metabolismo , Receptores de Superfície Celular/metabolismo , Sequência de Aminoácidos , Animais , Sequência de Bases , Regulação da Expressão Gênica no Desenvolvimento , Nácar/metabolismo , Especificidade de Órgãos , RNA Mensageiro/genética , Receptores de Superfície Celular/química , Receptores de Superfície Celular/genética , Solubilidade , Unionidae
18.
Talanta ; 202: 369-374, 2019 Sep 01.
Artigo em Inglês | MEDLINE | ID: mdl-31171197

RESUMO

Hypochloric acid (HOCl) plays vital roles in cell signaling and homeostasis which involved in many related diseases. Therefore, the accurate detected level of OCl- for discussing the complex contributions of OCl- to human health is of great significance. In this study, a novel mitochondrion-targeting fluorescent probe DMI bearing a carbon-carbon double bond as an OCl--responsive site has been developed. Probe DMI exhibited specific fluorescence response toward OCl- with fast response (within 4 s) and high sensitivity (detection limit is 0.05 µM), where the obvious color changes could be observed by the naked eye. More importantly, DMI could be applied in the bioimaging of OCl- in living A549 cells successfully benefited from its good sensing properties and low toxicity. Fluorescence co-localization test was further carried out and confirmed mitochondria-targetable ability of DMI which can be used for investigating physiological function of HOCl at organelle levels.


Assuntos
Corantes Fluorescentes/química , Ácido Hipocloroso/análise , Mitocôndrias/química , Imagem Óptica , Células A549 , Sobrevivência Celular/efeitos dos fármacos , Corantes Fluorescentes/síntese química , Corantes Fluorescentes/farmacologia , Humanos , Estrutura Molecular , Espectrofotometria
19.
Am J Transl Res ; 11(3): 1230-1240, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-30972158

RESUMO

Mesenchymal stem cells (MSCs) can be recruited to damaged tissues directly for regeneration. Exosomes, acting as an important ingredient of MSCs-involved intercellular communication through paracrine actions, also play significant roles in tissue damage repair and have a prospect of potential clinical application. It is generally recognized that MSC-derived exosomes (MSC-exosomes) enhance tissue regeneration and repair through reducing inflammatory responses, promoting proliferation, inhibiting apoptosis and facilitating angiogenesis. This review summarizes the positive effects of human umbilical cord mesenchymal stem cells (hucMSCs) and hucMSC-derived exosomes (hucMSC-exosomes) on tissue damage and the specific mechanisms of repair action.

20.
Fish Shellfish Immunol ; 89: 437-447, 2019 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-30980916

RESUMO

Proteinase inhibitors with the ability to inhibit specific proteinases are usually closely connected with the immune system. Interestingly, proteinase inhibitors are also a common ingredient in the organic matrix of mollusk shells. However, the molecular mechanism that underlies the role of proteinase inhibitors in immune system and shell mineralization is poorly known. In this study, a Kunitz serine proteinase inhibitor (HcKuPI) was isolated from the mussel Hyriopsis cumingii. HcKuPI was specifically expressed in dorsal epithelial cells of the mantle pallium and HcKuPI dsRNA injection caused an irregular surface and disordered deposition on the aragonite tablets of the nacreous layer. These results indicated that HcKuPI plays a vital role in shell nacreous layer biomineralization. Moreover, the expression pattern of HcKuPI during LPS challenge and pearl formation indicated its involvement in the antimicrobial process during pearl sac formation and nacre tablets accumulation during pearl formation. In the in vitro calcium carbonate crystallization assay, the addition of GST-HcKuPI increased the precipitation rate of calcium carbonate and induced the crystal overgrowth of calcium carbonate. Taken together, these results indicate that HcKuPI is involved in antimicrobial process during pearl formation, and participates in calcium carbonate deposition acceleration and morphological regulation of the crystals during nacreous layer formation. These findings extend our knowledge of the role of proteinase inhibitors in immune system and shell biomineralization.


Assuntos
Antibacterianos/metabolismo , Carbonato de Cálcio/metabolismo , Nácar/metabolismo , Inibidores de Proteases , Unionidae/genética , Unionidae/imunologia , Sequência de Aminoácidos , Animais , Sequência de Bases , Inibidores de Proteases/química , Inibidores de Proteases/metabolismo , Unionidae/metabolismo
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