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Magn Reson Med ; 85(1): 346-356, 2021 01.
Artigo em Inglês | MEDLINE | ID: mdl-32726485


PURPOSE: To explore the value of MRI texture analysis in evaluating the presence and severity of early renal ischemia-reperfusion injury (IRI). METHODS: Healthy New Zealand rabbits were used (IRI group, N = 54; control group, N = 8). Rabbits in the IRI group underwent left renal artery clamping for 60 minutes. Magnetic resonance imaging was performed before and at 1, 12, 24, and 48 hours after IRI. The relationship between MRI texture features and histopathology parameters was assessed using Pearson's correlation coefficients. The diagnostic performance of texture features in kidney differentiation at different time points was assessed by receiver operating characteristic curve analysis. RESULTS: T2 WI_S(3,-3)Inverse_Difference_Moment had the strongest correlation with brush border destruction, tubular epithelial edema, necrosis, and cast (r = 0.56, -0.58, 0.62, and 0.69, respectively; all P < .001). BOLD_S(4,-4)Correlation had the strongest correlation with interstitial inflammatory cell infiltration (r = 0.63, P < .001). SWI_S(4,4)Difference_Entropy had the strongest correlation with microvessel density (r = 0.61, P < .001). The areas under the curve for T2 WI_S(3,-3)Inverse_Difference_Moment, SWI_S(4,4)Difference_Entropy, and BOLD_S(4,-4)Correlation in kidney differentiation before IRI and that at 1 and 12 hours after reperfusion were 0.76, 0.72, and 0.70, respectively; the values before IRI and at 24 and 48 hours after reperfusion were 0.84, 0.81, and 0.69, respectively. The area under the curve for T2 WI_S(3,-3)Inverse_Difference_Moment in kidney differentiation at 1 and 12 hours after reperfusion and that at 24 and 48 hours after reperfusion was 0.66. CONCLUSION: Magnetic resonance imaging texture analysis can be used for evaluating the presence and severity of early renal IRI.

Wei Sheng Wu Xue Bao ; 49(12): 1576-83, 2009 Dec.
Artigo em Chinês | MEDLINE | ID: mdl-20222441


OBJECTIVE: To analyze the structural characteristics of tdh gene and its adjacent loci of Vibrio parahaemolyticus isolates from seafoods. METHODS: Long distance PCR and genome walking were used to amplify the DNA sequences flanking the tdh gene, and the sequences were analyzed by blastn against the NCBI database. RESULTS: The genetic structure of tdh-adjacent loci (VPA1312-VPA1327) from isolate ZS34 was similar to that of the reference strain RIMD2210633, with the nucleotide identity of 98.3%. The tdh gene of isolates FJ14 and WZ64 was located in the loci different from the reference strain and ZS34, and showed high nucleotide similarity to tdh3 gene. In the genome of isolate FJ14, tdh was 15kb away from the trh- ure cluster, with IS-like elements and transposase genes inserted therein. Isolate WZ64 lacked trh gene, but also harbored IS-like elements upstream of tdh. CONCLUSION: The tdh-adjacent loci of V. parahaemolyticus seafood isolates exhibit high diversity, an additional evidence of lateral gene transfer in this particular species.

Proteínas de Bactérias/genética , Alimentos Marinhos/microbiologia , Vibrio parahaemolyticus/genética , Vibrio parahaemolyticus/isolamento & purificação , Proteínas de Bactérias/química , Sequência de Bases , Genoma Bacteriano , Dados de Sequência Molecular , Alinhamento de Sequência , Vibrio parahaemolyticus/química
Food Microbiol ; 26(1): 103-11, 2009 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-19028313


In this study, we examined Listeria monocytogenes isolates from Chinese food sources in an attempt to gain further insights on the molecular characteristics and virulence potential of this important foodborne pathogen. Of the 88 L. monocytogenes food isolates recovered, 42 (47.7%) were of serovars 1/2a or 3a; 23 (26.1%) of serovars 1/2b or 3b; 15 (17.0%) of 1/2c or 3c; 6 (6.8%) of serovars 4b, 4d or 4e; and 2 (2.2%) of serovars 4a or 4c. In contrast to inlAB locus conserved in all serovars, internalin cluster between ascB and dapE varies with different serovars, with inlC2DE, inlGC2DE and inlGHE predominantly in serovars 1/2b or 4b, serovar 1/2a and serovar 1/2c. While inlF existed in all the inlGHE- and inlGC2DE-containing isolates but 17.4% of those having inlC2DE, lmo2026 existed in all the inlGHE-containing isolates but 20.0% of those bearing inlGC2DE, suggesting that inlF might have co-evolved with inlGC2DE and inlGHE while lmo2026 with inlGHE only. With the exception of serovar 4a isolate, most serovar isolates demonstrated remarkable ability to form plaques on L929 cells and produced significant mouse mortality irrespective of the internalin gene organization and whether an intact actA gene is present or not. These results indicate that majority of these food isolates may have the potential to cause human diseases if ingested via contaminated foods. Given that serovar 4b accounts for nearly half of human clinical listeriosis cases documented, the relative low proportion of serovar 4b food isolates suggests that this serovar is probably more tolerant of the adverse conditions in the host's stomach and/or more efficient in entering host cells than serovars 1/2a, 1/2b and 1/2c.

Proteínas de Bactérias/genética , Contaminação de Alimentos/análise , Listeria monocytogenes/isolamento & purificação , Listeria monocytogenes/patogenicidade , Animais , Sequência de Bases , Bioensaio , China , Análise por Conglomerados , DNA Bacteriano/química , DNA Bacteriano/genética , Feminino , Microbiologia de Alimentos , Humanos , Dose Letal Mediana , Listeria monocytogenes/classificação , Camundongos , Camundongos Endogâmicos ICR , Dados de Sequência Molecular , Distribuição Aleatória , Sorotipagem , Virulência