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1.
Sci Rep ; 10(1): 5245, 2020 Mar 23.
Artigo em Inglês | MEDLINE | ID: mdl-32251324

RESUMO

Cardiovascular disease (CVD) is the leading cause of death worldwide and a major public health concern. CVD prediction is one of the most effective measures for CVD control. In this study, 29930 subjects with high-risk of CVD were selected from 101056 people in 2014, regular follow-up was conducted using electronic health record system. Logistic regression analysis showed that nearly 30 indicators were related to CVD, including male, old age, family income, smoking, drinking, obesity, excessive waist circumference, abnormal cholesterol, abnormal low-density lipoprotein, abnormal fasting blood glucose and else. Several methods were used to build prediction model including multivariate regression model, classification and regression tree (CART), Naïve Bayes, Bagged trees, Ada Boost and Random Forest. We used the multivariate regression model as a benchmark for performance evaluation (Area under the curve, AUC = 0.7143). The results showed that the Random Forest was superior to other methods with an AUC of 0.787 and achieved a significant improvement over the benchmark. We provided a CVD prediction model for 3-year risk assessment of CVD. It was based on a large population with high risk of CVD in eastern China using Random Forest algorithm, which would provide reference for the work of CVD prediction and treatment in China.

2.
Int J Med Sci ; 17(4): 437-448, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-32174774

RESUMO

Traumatic brain injury (TBI) is a widespread central nervous system (CNS) condition and a leading cause of death, disability, and long-term disability including seizures and emotional and behavioral issues. To date, applicable diagnostic biomarkers have not been elucidated. MicroRNAs (miRNAs) are enriched and stable in exosomes in plasma. Therefore, we speculated that miRNAs in plasma exosomes might serve as novel biomarkers for TBI diagnosis and are also involved in the pathogenesis of TBI. In this study, we first isolated exosomes from peripheral blood plasma in rats with TBI and then investigated the alterations in miRNA expression in exosomes by high-throughput RNA sequencing. As a result, we identified 50 significantly differentially expressed miRNAs, including 31 upregulated and 19 downregulated miRNAs. Then, gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis revealed that the most highly correlated pathways that were identified were the MAPK signaling pathway, regulation of actin cytoskeleton, Rap1 signaling pathway and Ras signaling pathway. This study provides novel perspectives on miRNAs in peripheral blood plasma exosomes, which not only could be used as biomarkers of TBI diagnosis but could also be manipulated as therapeutic targets of TBI.

3.
Mol Brain ; 13(1): 11, 2020 01 28.
Artigo em Inglês | MEDLINE | ID: mdl-31992337

RESUMO

BACKGROUND: N6-methyladenosine (m6A) is the most prevalent post-transcriptional modification of eukaryotic mRNA. It has been reported that there is a stimulus-dependent regulation of m6A in the mammalian central nervous system in response to sensory experience, learning, and injury. The mRNA m6A methylation pattern in rat cortex after traumatic brain injury (TBI) has not been investigated. RESULTS: In this study, we conducted a genome-wide profiling of mRNA m6A methylation in rat cortex via methylated RNA immunoprecipitation sequencing (MeRIP-Seq). After TBI, the expressions of METTL14 and FTO were significantly down-regulated in rat cerebral cortex. Using MeRIP-Seq, we identified a total of 2165 significantly changed peaks, of which 1062 were significantly up-regulated and 1103 peaks were significantly down-regulated. These m6A peaks were located across 1850 genes. The analysis of both m6A peaks and mRNA expression revealed that there were 175 mRNA significantly altered methylation and expression levels after TBI. Moreover, it was found that functional FTO is necessary to repair neurological damage caused by TBI but has no effect on the spatial learning and memory abilities of TBI rats by using FTO inhibitor FB23-2. CONCLUSION: This study explored the m6A methylation pattern of mRNA after TBI in rat cortex and identified FTO as possible intervention targets in the epigenetic modification of TBI.

4.
Am J Emerg Med ; 38(1): 73-78, 2020 01.
Artigo em Inglês | MEDLINE | ID: mdl-31005392

RESUMO

OBJECTIVES: This study was designed to investigate whether an audiovisual feedback (AVF) device is beneficial for quality retention of chest compression (CC) after repetitive practices (RP). METHODS: After completion of a 45-min CC-only cardiopulmonary resuscitation (CPR) training, participants performed 3 sessions of practices on days 1, 3, and 7 under the guidance of an instructor with (RP + AVF) or without (RP) the AVF device. CC quality was determined after each session and was retested at 3 and 12 months. RESULTS: In total, ninety-seven third year university students participated in this study. CC quality was improved after 3 sessions in both the RP and RP + AVF groups. Retests at 3 months showed that the proportions of appropriate CC rate and correct hand position were significantly decreased in the RP group as compared with the last practice (p < 0.05). However, no significant changes in CC quality were observed in the RP + AVF group. However, the proportions of appropriate CC rate, depth, and complete recoil were significantly decreased after 12 months in both RP and RP + AVF groups (p < 0.05). There were no significant differences in these parameters between the RP and the RP + AVF groups at 12 months after RP. CONCLUSION: With RP, the use of an AVF device further improves initial CC skill acquisition and short-term quality retention. However, long-term quality retention is not statistically different between rescuers who receive verbal human feedback only and those who receive additional AVF device feedback after RP.


Assuntos
Recursos Audiovisuais , Reanimação Cardiopulmonar/educação , Competência Clínica , Retroalimentação , Parada Cardíaca Extra-Hospitalar/terapia , Prática Psicológica , Adulto , Feminino , Humanos , Masculino , Manequins , Retenção Psicológica , Fatores de Tempo
5.
Medicine (Baltimore) ; 98(49): e18248, 2019 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-31804354

RESUMO

Dementia among elderly is a serious problem worldwide. This study was conducted to estimate the prevalence and associated risk factors of dementia and mild cognitive impairment (MCI) in nursing homes (NHs) and common communities (CCs) among elderly in China.A cross-sectional survey was conducted in 4 communities across 12 cities in Southern China from May to November of 2014. Qualified psychiatrists and trained nurses carried out relevant diagnosis, assessments, interviews, and information collection. Screening test of mini-mental state examination was conducted among participants firstly, then confirmed diagnosis was carried out among the ones with positive results. Student t test, χ test, univariate, and multivariate logistic regression analysis were conducted to analyze data.A total of 2015 participants aged 65 or older were included in the final analysis; 908 came from NHs while 1107 came from CCs. The crude prevalence rates of dementia and MCI were 22.0% and 15.8%, respectively among all the participants. Dementia prevalence was 42.4% among those living in NHs, which was significantly higher than that of 5.3% in CCs (P < .0001). There were more moderate and severe dementia in NHs compared with CCs (P < .0001). It showed that older age, illiterate compared with high level of education (adjusted odds ratio, AOR = 3.32, 95% CI: 1.53-7.21), heavy drinking (AOR = 1.51 (1.00-2.24), having a medical history of diabetes (AOR = 1.41, 95% CI: 1.02-2.33), and stroke (AOR = 1.21, 95% CI: 1.01-1.23) were associated with dementia in NHs, and middle socioeconomic status might be a protective factor for dementia (AOR = 0.33, 95% CI: 0.21-0.51).The problem of senile dementia in NHs is much more serious than our estimation, and there are not enough trained nursing staffs in NHs. More population-based strategies in NHs, including conducting cognitive screening accompanied with routine physical examination among elderly population, carrying out related primary prevention policies and public health services, and paying attention to some modifiable associated risk factors such as heavy smoking and drinking are needed.


Assuntos
Doença de Alzheimer/epidemiologia , Disfunção Cognitiva/epidemiologia , Vida Independente , Casas de Saúde , Idoso , Idoso de 80 Anos ou mais , China/epidemiologia , Estudos Transversais , Feminino , Avaliação Geriátrica , Humanos , Masculino , Prevalência , Fatores de Risco
6.
Artigo em Inglês | MEDLINE | ID: mdl-31341504

RESUMO

Acupoint catgut embedding is a useful therapy for weight management and widely applied in China. This review aimed to systematically evaluate the effects of acupoint catgut embedding on abdominal obesity. We searched the PubMed, Cochrane-Library, Embase, OVID, MEDLINE, ISI (web of science), Wanfang, VIP, CBM, and CNKI for randomized controlled trials that used acupoint catgut embedding to treat abdominal obesity before April 2019 with the language restriction of Chinese and English. The combination subject terms of abdominal obesity (or central obesity) and acupoint catgut embedding (or catgut implantation, catgut embedding) were used. We found 15 studies involving 1584 individuals. When acupoint catgut embedding plus electroacupuncture is compared with electroacupuncture alone, significant reductions in improvement rate (RR = 1.03, 95% CI = 0.99~1.08), body weight (MD = 5.20, 95%CI = 1.16~9.25), body mass index (MD = 1.73, 95%CI = 0.70~2.76), waist circumference (MD = 2.91, 95%CI = 1.36~4.46), and hip circumference (MD = 1.06, 95%CI = -0.18~2.30) were found. Mean values of body weight by acupoint catgut embedding were 1.35 kg compared with electroacupuncture. Less adverse events were reported in all included articles. In summary, pooled outcomes of acupoint catgut embedding presented a tendency of equal effects to other kinds of acupuncture, whereas acupoint catgut embedding plus electroacupuncture is more effective for abdominal obesity. This study is registered with PROSPERO 2017 (CRD42017082357).

7.
Ecotoxicol Environ Saf ; 179: 249-256, 2019 Sep 15.
Artigo em Inglês | MEDLINE | ID: mdl-31054378

RESUMO

The functional role of 1,25-vitamin D3 in cooking oil fumes (COFs)-derived PM2.5-induced cell damage is largely unexplored. The present study investigated the protective role of 1,25-vitamin D3 against cell injury by possible involvement of JAK/STAT and NF-κB signaling pathways in cardiomyocytes. Cell viability was measured using CCK-8 assay, and cell apoptosis was analyzed by flow cytometry, qRT-PCR and Western blot in cultured rat neonatal cardiomyocytes treated with 1,25-vitamin D3 and COFs-derived PM2.5. Expressions of JAK/STAT and NF-κB signaling pathway were measured by Western blot. The results suggested that treatment with COFs-derived PM2.5 significantly decreased cell viability and increased apoptosis and oxidative stress in cultured rat neonatal cardiomyocytes. 1,25-vitamin D3 pretreatment alleviated the cell injury by increasing cell viability and decreasing apoptosis in the cardiomyocytes. 1,25-vitamin D3 pretreatment also decreased the ROS level and inflammation in the cardiomyocytes. Furthermore, 1,25-vitamin D3 pretreatment alleviated COFs-derived PM2.5-evoked elevation of JAK/STAT and NF-κB signaling pathways. Our study showed that 1,25-vitamin D3 pretreatment protected cardiomyocytes from COFs-derived PM2.5-induced injury by decreasing ROS, apoptosis and inflammation level via activations of the JAK/STAT and NF-κB signaling pathways.


Assuntos
Poluentes Atmosféricos/toxicidade , Anti-Inflamatórios/farmacologia , Colecalciferol/farmacologia , Miócitos Cardíacos/efeitos dos fármacos , Material Particulado/toxicidade , Animais , Apoptose/efeitos dos fármacos , Sobrevivência Celular/efeitos dos fármacos , Culinária/métodos , Miócitos Cardíacos/metabolismo , NF-kappa B/metabolismo , Estresse Oxidativo/efeitos dos fármacos , Tamanho da Partícula , Ratos , Transdução de Sinais/efeitos dos fármacos
8.
Medicine (Baltimore) ; 98(8): e13918, 2019 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-30813122

RESUMO

RATIONALE: Spasticity is a common issue in chronic stroke. To date, no study has reported the long-term (up to 1 year) outcomes of Fu's subcutaneous needling in combination with constraint-induced movement therapy in chronic stroke. This report describes the successful addition of acupuncture on spasticity and arm function in a patient with chronic stroke and arm paresis. PATIENT CONCERNS: The patient suffered an infarction in the right posterior limb of the internal capsule 1 year ago, which resulted in hemiparesis in his left (nondominant) hand and arm. The only limitation for constraint-induced movement therapy was insufficient finger extension. The patient was unable to voluntarily extend his interphalangeal or metacarpophalangeal joints beyond the 10 degrees required for constraint-induced movement therapy. However, his muscle tension did not change after the BTX type A injection. DIAGNOSES: A 35-year-old male experienced arm paresis after an infarction in the right posterior limb of the internal capsule 1 year before the intervention. INTERVENTIONS: The BTX type A injection did not work, so the patient received Fu's subcutaneous needling as an alternative therapy before 5 h of constraint-induced movement therapy for 12 weekdays. OUTCOMES: All outcome measures (Modified Ashworth Scale, Fugl-Meyer Assessment, Action Research Arm Test, and Motor Activity Log) substantially improved over the 1-year period. Moreover, during the observation period, the patient's muscle tone and arm function did not worsen. LESSONS: As a result of a reduction in spasticity, a reduction of learned nonuse behaviors, or use-dependent plasticity after the combined therapy, the arm functions include volitional movements, and coordination or speed of movements in the paretic arm have been improved. However, we cannot rule out the possibility of an influence of the passage of time or the Hawthorne effect. The costs of the treatment of stroke may be reduced, if this combined therapy proved useful in future controlled studies.


Assuntos
Terapia por Acupuntura/métodos , Técnicas de Exercício e de Movimento/métodos , Espasticidade Muscular/reabilitação , Paresia/reabilitação , Reabilitação do Acidente Vascular Cerebral/métodos , Terapia por Acupuntura/economia , Adulto , Braço/fisiopatologia , Doença Crônica , Terapia Combinada , Técnicas de Exercício e de Movimento/economia , Seguimentos , Humanos , Masculino , Espasticidade Muscular/etiologia , Agulhas , Paresia/etiologia , Acidente Vascular Cerebral/complicações , Resultado do Tratamento
9.
BMC Plant Biol ; 19(1): 48, 2019 Feb 01.
Artigo em Inglês | MEDLINE | ID: mdl-30709373

RESUMO

BACKGROUND: Salinity-alkalinity stress is one of the major abiotic stresses affecting plant growth and development. γ-Aminobutyrate (GABA) is a non-protein amino acid that functions in stress tolerance. However, the interactions between cellular redox signaling and chlorophyll (Chl) metabolism involved in GABA-induced salinity-alkalinity stress tolerance in plants remains largely unknown. Here, we investigated the role of GABA in perceiving and regulating chlorophyll biosynthesis and oxidative stress induced by salinity-alkalinity stress in muskmelon leaves. We also evaluated the effects of hydrogen peroxide (H2O2), glutathione (GSH), and ascorbate (AsA) on GABA-induced salinity-alkalinity stress tolerance. RESULTS: Salinity-alkalinity stress increased malondialdehyde (MDA) content, relative electrical conductivity (REC), and the activities of superoxide dismutase (SOD), ascorbate peroxidase (APX) and dehydroascorbate reductase (DHAR). Salinity-alkalinity stress decreased shoot dry and fresh weight and leaf area, reduced glutathione and ascorbate (GSH and AsA) contents, activities of glutathione reductase (GR) and monodehydroascorbate reductase (MDAR). By contrast, pretreatment with GABA, H2O2, GSH, or AsA significantly inhibited these salinity-alkalinity stress-induced effects. The ability of GABA to relieve salinity-alkalinity stress was significantly reduced when the production of endogenous H2O2 was inhibited, but was not affected by inhibiting endogenous AsA and GSH production. Exogenous GABA induced respiratory burst oxidase homologue D (RBOHD) genes expression and H2O2 accumulation under normal conditions but reduced the H2O2 content under salinity-alkalinity stress. Salinity-alkalinity stress increased the accumulation of the chlorophyll synthesis precursors glutamate (Glu), δ-aminolevulinic acid (ALA), porphobilinogen (PBG), uroporphyrinogen III (URO III), Mg-protoporphyrin IX (Mg-proto IX), protoporphyrin IX (Proto IX), protochlorophyll (Pchl), thereby increasing the Chl content. Under salinity-alkalinity stress, exogenous GABA increased ALA content, but reduced the contents of Glu, PBG, URO III, Mg-proto IX, Proto IX, Pchl, and Chl. However, salinity-alkalinity stress or GABA treated plant genes expression involved in Chl synthesis had no consistent trends with Chl precursor contents. CONCLUSIONS: Exogenous GABA elevated H2O2 may act as a signal molecule, while AsA and GSH function as antioxidants, in GABA-induced salinity-alkalinity tolerance. These factors maintain membrane integrity which was essential for the ordered chlorophyll biosynthesis. Pretreatment with exogenous GABA mitigated salinity-alkalinity stress caused excessive accumulation of Chl and its precursors, to avoid photooxidation injury.


Assuntos
Clorofila/biossíntese , Cucurbitaceae/metabolismo , Oxirredução/efeitos dos fármacos , Ácido gama-Aminobutírico/farmacologia , Ascorbato Peroxidases/metabolismo , Cucurbitaceae/efeitos dos fármacos , Cucurbitaceae/fisiologia , Peróxido de Hidrogênio/metabolismo , Concentração de Íons de Hidrogênio , Malondialdeído/metabolismo , Oxirredutases/metabolismo , Estresse Salino , Estresse Fisiológico , Superóxido Dismutase/metabolismo
10.
Se Pu ; 37(2): 143-148, 2019 Feb 08.
Artigo em Chinês | MEDLINE | ID: mdl-30693721

RESUMO

A novel polar urea-propyl-C30 (TPU-C30) reversed stationary phase was prepared by using 3-ureidopropyltrimethoxysilane as a coupling agent. The characteristics of TPU-C30 were analyzed by scanning electron microscopy, elemental analysis, infrared spectroscopy and thermal analysis. The results showed that the TPU-C30 stationary phase was successfully prepared. Furthermore, the relative deviations of the element contents of TPU-C30 were within 5% (n=3), which indicated that the proposed synthesis had good repeatability. The chromatographic performances in the stationary phase were evaluated by using different polar compounds, positional isomers and basic compounds as solute probes. Compared to the conventional C18 and C30 columns, the TPU-C30 stationary phase had different selectivity and better shape selection. The TPU-C30 stationary phase can be applied in various fields.

11.
World Neurosurg ; 123: e465-e473, 2019 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-30500588

RESUMO

OBJECTIVE: The latest World Health Organization data showed that stroke was the highest mortality in China, accounting for 23.7% of the total mortality from 2000 to 2012. Intracerebral hemorrhage (ICH) was the most deadly and incurable type of stroke. In the Qinghai-Tibetan Plateau, the incidence of stroke was relatively higher. Several studies showed that the shape and heterogeneity of hematoma and image markers on brain computed tomography scan had predictive effects on hematoma expansion (HE). The study aimed to find relative factors and established a nomogram model to predict the HE of ICH. METHODS: All patients with ICH in Qinghai Provincial People's Hospital from January 1, 2012, to May 22, 2018, were continuously collected. A total of 402 patients were included in the study. This was a single-center retrospective study. Univariate and binary logistic regression analysis were performed to screen out the independent predictors that were significantly associated with HE. RESULTS: The total incidence of HE in ICH was 30.9%, whereas the incidence of HE in the basal ganglia and nonbasal ganglia was 36.4% and 17.2%, respectively. Diabetes, basal ganglia hemorrhage, time of onset to baseline computed tomography, island sign, blend sign, black hole sign, and swirl sign were independent predictors of HE. Based on these predictors, a nomogram model was established and the accuracy was 81.6%, the sensitivity was 91.1%, and the specificity was 70.5%. CONCLUSIONS: This model had a high accuracy of predicting HE in the Qinghai-Tibetan Plateau. Because this model is noninvasive, rapid, and low cost, it is easy to promote and has wide application prospects in clinical practice.


Assuntos
Hemorragia Cerebral/patologia , Hematoma/patologia , Idoso , Hemorragia Cerebral/etnologia , China/epidemiologia , China/etnologia , Feminino , Hematoma/etnologia , Humanos , Incidência , Masculino , Pessoa de Meia-Idade , Nomogramas , Curva ROC , Estudos Retrospectivos , Índice de Gravidade de Doença , Acidente Vascular Cerebral/etnologia , Acidente Vascular Cerebral/patologia , Tibet/epidemiologia , Tibet/etnologia , Tomografia Computadorizada por Raios X
12.
J Altern Complement Med ; 25(1): 73-78, 2019 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-30136858

RESUMO

OBJECTIVE: This study aims to investigate the effect of t'ai chi exercise on hypertension in young and middle-aged in-service staff. METHODS: A total of 208 subjects with grade 1 hypertension were enrolled into this study. These subjects were randomly divided into two groups: research group and control group (n = 104, each). On the basis of general daily lifestyle intervention, subjects in the research group underwent 24-Style Simplified t'ai chi exercise for 3 months, whereas subjects in the control group underwent general daily lifestyle intervention. All subjects were followed up at the first and third month of intervention. The body mass index (BMI), blood pressure, blood lipid, and other indexes were measured before and after the intervention, and quality of life was evaluated. RESULTS: (1) In the research group, after 1 month of exercise, systolic blood pressure (SBP), heart rate (HR), triglyceride (TG), total cholesterol (TC), and low-density lipoprotein cholesterol (LDL-C) significantly decreased (p < 0.05), while BMI, blood glucose (Glu), diastolic blood pressure (DBP), and pulse pressure (PP) did not significantly change. Furthermore, after 3 months of exercise, BMI, HR, SBP, DBP, PP, TG, TC, LDL-C, and Glu all significantly decreased (p < 0.05). (2) Moreover, the quality of life of subjects in the research group obviously improved after 3 months of t'ai chi exercise (p < 0.05). CONCLUSIONS: t'ai chi exercise can reduce the level of blood pressure in young and middle-aged in-service staff with grade 1 hypertension, control weight, slow down the HR, improve metabolism, and improve quality of life. t'ai chi is an exercise suitable for in-service hypertension subjects.


Assuntos
Hipertensão/terapia , Tai Ji , Adulto , Pressão Sanguínea/fisiologia , Feminino , Frequência Cardíaca/fisiologia , Humanos , Lipídeos/sangue , Masculino , Pessoa de Meia-Idade , Qualidade de Vida/psicologia
13.
World Neurosurg ; 2018 Dec 27.
Artigo em Inglês | MEDLINE | ID: mdl-30593962

RESUMO

OBJECTIVE: By extracting clinical and computed tomography imaging data of patients with acute subdural hematoma (ASDH), factors that were significantly associated with poor prognosis were screened and a nomogram model was established and validated. METHODS: All patients with ASDH who underwent subdural hematoma removal and decompressive craniectomy from January 2014 to March 2018 in Qinghai Provincial People's Hospital were continuously collected. Finally, 124 patients were included in the study. According to the Glasgow Outcome Scale at 3 months after operation, patients were divided into a good prognosis group and a poor prognosis group. RESULTS: Univariate and binary logistic regression analysis were performed to screen out independent predictors that were significantly associated with poor prognosis of ASDH. On the basis of these factors, a nomogram model was established. CONCLUSIONS: The nomogram model had high accuracy for predicting poor prognosis in patients with ASDH, and it was easy to promote. In the future, large sample and multicenter prospective studies are necessary to complement and identify the results.

14.
EMBO Rep ; 19(10)2018 10.
Artigo em Inglês | MEDLINE | ID: mdl-30126922

RESUMO

Progressive attrition of telomeres triggers DNA damage response (DDR) and limits the regenerative capacity of adult stem cells during mammalian aging. Intriguingly, telomere integrity is not only determined by telomere length but also by the epigenetic status of telomeric/sub-telomeric regions. However, the functional interplay between DDR induced by telomere shortening and epigenetic modifications in aging remains unclear. Here, we show that deletion of Gadd45a improves the maintenance and function of intestinal stem cells (ISCs) and prolongs lifespan of telomerase-deficient mice (G3Terc -/-). Mechanistically, Gadd45a facilitates the generation of a permissive chromatin state for DDR signaling by inducing base excision repair-dependent demethylation of CpG islands specifically at sub-telomeric regions of short telomeres. Deletion of Gadd45a promotes chromatin compaction in sub-telomeric regions and attenuates DDR initiation at short telomeres of G3Terc -/- ISCs. Treatment with a small molecule inhibitor of base excision repair reduces DDR and improves the maintenance and function of G3Terc -/- ISCs. Taken together, our study proposes a therapeutic approach to enhance stem cell function and prolong lifespan by targeting epigenetic modifiers.


Assuntos
Proteínas de Ciclo Celular/genética , Epigênese Genética/genética , Proteínas Nucleares/genética , RNA/genética , Telomerase/genética , Telômero/genética , Envelhecimento/genética , Envelhecimento/metabolismo , Animais , Ilhas de CpG/genética , Dano ao DNA/genética , Mucosa Intestinal/metabolismo , Camundongos , Camundongos Knockout , Células-Tronco/metabolismo
15.
Sheng Wu Yi Xue Gong Cheng Xue Za Zhi ; 35(1): 45-48, 2018 02 25.
Artigo em Chinês | MEDLINE | ID: mdl-29745599

RESUMO

Due to the decline of motor ability and the impact of the diseases, abnormalities in gait is common in the elderly population, which will raise the risk of fall and cause serious injury. This study focuses on the analysis of the gait kinematics parameters of normal adults' gait, aiming to investigate the characteristics of gait parameters in different age groups and to explore the role of gait parameters in motor function assessment and clinical diagnosis. Based on the gait data gained by electronic walkway, the relationship among the toe out angles and their correlation with age and gender etc. were quantitatively analyzed. The results show that most normal subjects walk with positive toe out angles, and the angles increase with age. Such changes are slow in the young and middle age groups. However, the elevations of the left out toe angle and the angles between the feet are statistically significant after entering elder age ( >60 years). The results also suggest that the angle between the feet is a kind of practical gait parameter for varying applications. This study concludes that feet angle analysis is potential to provide a convenient and quantitative tool for the assessment of lower limb motor ability and the diagnosis of knee joint diseases.

16.
World Neurosurg ; 116: e759-e765, 2018 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-29807174

RESUMO

OBJECTIVE: To evaluate relationship between gray-white matter ratio (GWR) on computed tomography and prognosis in patients with extra-axial hematoma. METHODS: Patients with extra-axial hematoma who had undergone surgical treatment at Qinghai Provincial People's Hospital from September 2016 to December 2017 were included. GWR values were calculated by measuring values of gray matter and white matter in Hounsfield units in noninjury sites of basal ganglia. According to Glasgow Outcome Scale score at 3 months after operation, patients were divided into good prognosis (Glasgow Outcome Scale score 4-5) and poor prognosis (Glasgow Outcome Scale score 1-3) groups. RESULTS: Of 103 patients who met inclusion criteria and were analyzed, 24 had with a poor outcome, including 5 deaths, and 79 patients had a good outcome. There were significant differences between the 2 groups in terms of age, type of hematoma, admission Glasgow Coma Scale score, and presence of hernia (all P < 0.05). Postoperative HU values of posterior capsule, postoperative GWR values, and difference between preoperative and postoperative GWR values had statistical significance (all P < 0.05). Receiver operating characteristic curve showed that difference between preoperative and postoperative GWR values was the best predictor of poor outcome. CONCLUSIONS: The difference between preoperative or postoperative GWR values has a higher sensitivity and greater area under the curve to predict patient outcome. Measuring the difference between preoperative and postoperative GWR values may be useful as an objective early predictor of outcome in patients with extra-axial hematoma. Larger samples and multicenter prospective studies are still required.


Assuntos
Substância Cinzenta/diagnóstico por imagem , Hematoma/diagnóstico por imagem , Tomografia Computadorizada por Raios X/métodos , Substância Branca/diagnóstico por imagem , Adulto , Feminino , Escala de Coma de Glasgow , Hematoma/cirurgia , Humanos , Masculino , Pessoa de Meia-Idade , Valor Preditivo dos Testes , Curva ROC , Estudos Retrospectivos , Adulto Jovem
17.
PLoS One ; 13(2): e0193037, 2018.
Artigo em Inglês | MEDLINE | ID: mdl-29447233

RESUMO

Notch signaling pathway is involved in many physiological and pathological processes. The γ-secretase inhibitor DAPT inhibits Notch signaling pathway and promotes nerve regeneration after cerebral ischemia. However, neuroprotective effects of DAPT against acute craniocerebral injury remain unclear. In this study, we established rat model of acute craniocerebral injury, and found that with the increase of damage grade, the expression of Notch and downstream protein Hes1 and Hes5 expression gradually increased. After the administration of DAPT, the expression of Notch, Hes1 and Hes5 was inhibited, apoptosis and oxidative stress decreased, neurological function and cognitive function improved. These results suggest that Notch signaling can be used as an indicator to assess the severity of post-traumatic brain injury. Notch inhibitor DAPT can reduce oxidative stress and apoptosis after acute craniocerebral injury, and is a potential drug for the treatment of acute craniocerebral injury.


Assuntos
Traumatismos Craniocerebrais/prevenção & controle , Diaminas/farmacologia , Fármacos Neuroprotetores/farmacologia , Receptores Notch/antagonistas & inibidores , Tiazóis/farmacologia , Animais , Proteínas Reguladoras de Apoptose/genética , Proteínas Reguladoras de Apoptose/metabolismo , Fatores de Transcrição Hélice-Alça-Hélice Básicos/antagonistas & inibidores , Fatores de Transcrição Hélice-Alça-Hélice Básicos/genética , Fatores de Transcrição Hélice-Alça-Hélice Básicos/metabolismo , Lesões Encefálicas Traumáticas/patologia , Lesões Encefálicas Traumáticas/fisiopatologia , Lesões Encefálicas Traumáticas/prevenção & controle , Traumatismos Craniocerebrais/patologia , Traumatismos Craniocerebrais/fisiopatologia , Modelos Animais de Doenças , Regulação para Baixo/efeitos dos fármacos , Masculino , Estresse Oxidativo/efeitos dos fármacos , RNA Mensageiro/genética , RNA Mensageiro/metabolismo , Ratos , Ratos Sprague-Dawley , Receptores Notch/genética , Receptores Notch/metabolismo , Proteínas Repressoras/antagonistas & inibidores , Proteínas Repressoras/genética , Proteínas Repressoras/metabolismo , Transdução de Sinais/efeitos dos fármacos , Fatores de Transcrição HES-1/antagonistas & inibidores , Fatores de Transcrição HES-1/genética , Fatores de Transcrição HES-1/metabolismo
18.
Aging Clin Exp Res ; 30(7): 775-781, 2018 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-29022271

RESUMO

BACKGROUND: Clock-drawing test (CDT) is widely used but lack of a suitable scoring method. AIMS: To compare the validity of six common CDT scoring methods and to find out the best one. METHODS: The drawing CDT was administered in a Chinese nursing-home inhabitants living on the mainland including 110 dementia, 118 MCI (mild cognitive impairment), and 133 random normal. We calculated the sensitivity and specificity of six scoring methods and applied the receiver-operating characteristic (ROC) curve statistic, including determining the area under the curve (AUC). RESULTS: (1) All six CDT scoring methods had a value of sensitivity higher than 80% and a specificity of 60% except Jouk and Tuokko. Freund got the highest sensitivity (92.73%) of that five for the testing of dementia and high sensitivity (82.20%) for MCI with an acceptable specificity (70.68%). (2) The AUC (area under the ROC curve) of all six CDT methods was over 0.8 for dementia, and for MCI, only Jouk and Tuokko were lower than 0.8. Mendez had the largest AUC of 0.872 for MCI, which closely followed by Freund with 0.859. (3) Freund predicted dementia best but had no significant difference (p > 0.05); it only had significant difference with Jouk and Tuokko (p < 0.001) and the method in MoCA (p < 0.05) for both MCI and cognitive impairment. CONCLUSIONS: Our study suggests that Freund scoring method could be the best one among the six evaluated scoring methods within our setting.


Assuntos
Disfunção Cognitiva/diagnóstico , Demência/diagnóstico , Testes Neuropsicológicos , Idoso , Idoso de 80 Anos ou mais , Estudos de Casos e Controles , Demência/psicologia , Feminino , Humanos , Masculino , Casas de Saúde , Curva ROC , Projetos de Pesquisa , Sensibilidade e Especificidade
19.
Aging Ment Health ; 22(8): 942-946, 2018 08.
Artigo em Inglês | MEDLINE | ID: mdl-28485630

RESUMO

OBJECTIVE: Dementia is one of the leading causes of dependence in the elderly. This study was conducted to estimate diagnostic performance of dementia screening tests including Mini-Mental State Examination (MMSE), Mini-Cog, Clock Drawing Test (CDT) and Ascertain Dementia 8 questionnaire (AD8) by Bayesian models. METHOD: A total of 2015 participants aged 65 years or more in eastern China were enrolled. The four screening tests were administered and scored by specifically trained psychiatrists. The prior information of sensitivity and specificity of every screening test was updated via Bayes' theorem to a posterior distribution. Then the results were compared with the estimation based on National Institute of Aging-Alzheimer's Association criteria (NIA-AA). RESULTS: The diagnostic characteristics of Mini-Cog, including sensitivity, specificity, PPV, NPV, especially the Youden index, performed well, even better than the combinations of several screening tests. CONCLUSION: The Mini-Cog with excellent screening characteristics, spending less time, could be considered to be used as a screening test to help to screen patients with cognitive impairment or dementia early. And Bayesian method was shown to be a suitable tool for evaluating dementia screening tests. CONCLUSION: The Mini-Cog with excellent screening characteristics, spending less time, could be considered to be used as a screening test to help to screen patients with cognitive impairment or dementia early. And Bayesian method was shown to be a suitable tool for evaluating dementia screening tests.


Assuntos
Disfunção Cognitiva/diagnóstico , Demência/diagnóstico , Testes de Estado Mental e Demência/normas , Idoso , Idoso de 80 Anos ou mais , China , Feminino , Humanos , Masculino , Modelos Estatísticos , Sensibilidade e Especificidade
20.
Folia Neuropathol ; 56(4): 337-345, 2018.
Artigo em Inglês | MEDLINE | ID: mdl-30786671

RESUMO

INTRODUCTION: Present investigation determines the beneficial effect of picroliv against lipopolysaccharide (LPS)-induced neuronal inflammation and injury. MATERIAL AND METHODS: Neuronal injury was induced by LPS 250 µg/kg, i.p. for the period of one week, and picroliv 12.5 and 25 mg/kg was given i.p. 30 min prior to the administration of LPS for the duration of 12 days. The effect of picroliv was determined on the cognitive function by Morris water maze (MWM). Mediators of inflammation were estimated by using enzyme-linked immunosorbent assay (ELISA) and western blot, reverse transcription polymerase chain reaction (RT-PCR) and immunohistochemical analysis was done to determine the expressions of several proteins. RESULTS: Data of the study reveal that picroliv ameliorates the reduced memory impairment and cognitive dysfunction in LPS-induced mice. Moreover, expressions of inflammatory protein and -amyloid protein and level of inflammatory mediators were found to be reduced in the picroliv-treated group as compared to the negative control group. Data of RT-PCR reveal that the gene of Toll-like receptor 4 (TLR-4), -synuclein, neurotrophic factor (BDNF) and interleukin-1 (IL-1) protein were also decreased in the picroliv-treated group as compared to the negative control group. In addition picroliv attenuates the altered level of nuclear factor-kB(p-NF-kB), amyloid- (A), -synuclein and glial fibrillary acidic protein (GFAP) positive cells in the brain of LPS-induced mice. CONCLUSIONS: The report concludes that picroliv protects the neuroinflammation and injury in LPS-induced mice by regulating the inflammatory pathway.


Assuntos
Astrócitos/efeitos dos fármacos , Cinamatos/farmacologia , Glicosídeos/farmacologia , Microglia/efeitos dos fármacos , NF-kappa B/metabolismo , Receptor 4 Toll-Like/metabolismo , Ácido Vanílico/farmacologia , Peptídeos beta-Amiloides/metabolismo , Animais , Astrócitos/metabolismo , Disfunção Cognitiva/induzido quimicamente , Disfunção Cognitiva/tratamento farmacológico , Inflamação/tratamento farmacológico , Inflamação/metabolismo , Lipopolissacarídeos/farmacologia , Camundongos , Microglia/metabolismo , NF-kappa B/efeitos dos fármacos , Receptor 4 Toll-Like/efeitos dos fármacos
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