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1.
J Hazard Mater ; 383: 121124, 2020 Feb 05.
Artigo em Inglês | MEDLINE | ID: mdl-31505426

RESUMO

Due to potential adverse effects of excessive fluorine (F), interests regarding considerable accumulation of F in agricultural soils from application of fertilizers are increasing continually. However, less detailed information of hazard and risk of F in fertilizers to human directly by hand-to-mouth pathway can be obtained. Herein, the bioaccessibility of F (Fbio) in fertilizers is determined by Biomimetic Whole Digestion-Plasma in-vitro Method (BWDPM), which is developed to detect the behaviour of bioaccessible F in both the whole digestive system and plasma for the first time. Observations of higher Fbio in small intestinal phases (4.35-56.33%), large intestine (1.01-40.52%) and plasma (8.07-66.70%) yielded them as the major phases which are faced with higher exposure risk of F. Compared with phosphate fertilizer, more bioaccessible F was observed in compound fertilizer. Some studied factors, such as sweat and food, yield insignificant influences on Fbio, whereas can affect the exposure estimations of F considerably. Exposure risks based on Fbio of fertilizers should be highly concerned, especially the occupational exposure to local farmers. In a word, more explicit and realistic information of the potential risk of F on human health could be obtained by the introduction of Fbio.

2.
J Biomol Struct Dyn ; : 1-10, 2019 Nov 13.
Artigo em Inglês | MEDLINE | ID: mdl-31684837

RESUMO

Norovirus GII.4 variants, a genotype in genogroup II belonging to the genus Norovirus, is a single-strand positive sense RNA containing three open reading frames (ORF1, ORF2 and ORF3) and is the most important pathogen causing nonbacterial gastroenteritis outbreaks. By using bioinformatic softwares such as Codon W, SPSS and so on, a total of 292 strains of the viruses isolated from 1974 to 2016 were analyzed for nucleotide composition and synonymous codon usage in each ORF. The result shows that it is enriched for A over the other bases in nucleotide composition, G behind the other bases in the 3rd site of all synonymous codons in the three ORFs. The patterns of nucleotide composition and codon bias of ORF2 are similar to those of ORF3 and different from those of ORF1. There are generally UpA motif and CpG motif in the codons with the lowest proportion. Correspondence analysis indicates that the codon usage may be changing over a certain time period for ORF1 in 2006 and 2012, ORF2 in 2012, and ORF3 in 2013. ENC (effective number of codons) plot and other analyses indicate that both natural selection and mutational pressure play partly roles in the ORFs, but natural selection is more important for ORF2 and ORF3. Besides, we also found all optimal codons in the ORFs. The study provides a basic understanding of the mechanism for norovirus GII.4 codon usage bias. AbbreviationsORFOpen Reading FrameENCEffective Number of CodonsCOAcorrespondence analysisRSCURelative Synonymous Codon UsageCAICodon Adaptation IndexCBICodon Bias IndexFopfrequency of optimal codonsL_symnumber of synonymous codonsL_aalength amino acidsGRAVYgrand average of hydropathicityAromaaromaticityCommunicated by Ramaswamy H. Sarma.

3.
FEMS Microbiol Lett ; 2019 Nov 07.
Artigo em Inglês | MEDLINE | ID: mdl-31697369

RESUMO

Bacteria, especially pathogenic bacteria, were detected in order to estimate the safety of drinking water distribution systems (DWDS). 16 biofilms and 12 water samples (6 retained and 6 flowing) were collected from a city DWDS in Eastern China. Biofilms were observed using scanning electron microscopy. Cultivable bacteria of biofilms were counted by heterotrophic plate counts, ranging from 3.61 × 101 to 1.67 × 106 CFU·cm-2. Coliforms, Salmonella, Shigella, Vibrio, and Legionella were separated by EMB agar, Salmonella chromogenic medium, Shigella chromogenic medium, TCBS agar, BCYE agar, and 13/16, 8/16, 7/16, 6/16, 0/16 biofilm samples were found positive respectively. Retained and flowing water samples were collected to estimate the influence of hydrodynamic conditions on biofilm detachment. All 6 retained water samples were positive for bacteria, count ranged from 1.2 × 103 to 2.8 × 104 CFU·mL-1 and 2/6, 3/6, 2/6, 0/6, 0/6 samples were positive for coliforms, Salmonella, Shigella, Legionella, and Vibrio, respectively. While only 3 of 6 flowing water samples were bacteria positive, counts ranged from 102 to 103 CFU·mL-1, 2/6 were coliforms positive, no pathogens under testing were detected. The results show that there are pathogens in DWDS biofilms, which can cause health related problems if detached from their surface.

4.
Environ Int ; : 105000, 2019 Nov 04.
Artigo em Inglês | MEDLINE | ID: mdl-31699440

RESUMO

Inhalation from ambient air and cigarette smoke is a common route of human exposure to polycyclic aromatic hydrocarbons (PAHs). Little information is available regarding hepatotoxicities of inhaled PAHs so for. In this study, we evaluated the toxic effects of intratracheally instilled benzo[a]pyrene (B[a]P) on hepatic lipid metabolism of C57BL/6 mice at relevant environmental exposure levels by using two different mass-based lipidomics approaches. The results of mass spectrometry imaging analysis showed that both the abundance and spatial distribution of several lysophosphatidylcholine (LysoPC), phosphatidylcholine (PC) and sphingomyelin (SM) in the liver section were different and changed after inhalation exposure to B[a]P. Liquid chromatography coupled with mass spectrometry-based lipidomics analysis and multivariate statistical analysis found that B[a]P exposure markedly altered glycerophospholipids, glycerolipids, and fatty acid metabolism in the mouse liver, with increasing of triacylglycerol (TG), phosphatidylinositol (PI) and PC, and decreasing of LysoPCs phosphatidylethanolamines (PEs), lysophosphatidylethanolamine (LysoPEs), free fatty acids (FFAs) and eicosanoids. B[a]P-induced lipid metabolic disorders showed a time-dependent effect, which generated three response trajectories with different change trends. Consequently, B[a]P exposure induced alteration of hepatic lipids by promoting the uptake from blood or the biosynthesis and transformation in the liver, might contribute to non-alcoholic fatty liver disease, hepatocyte membrane injury, inflammation, and signal system disturbance.

6.
ACS Chem Neurosci ; 2019 Nov 22.
Artigo em Inglês | MEDLINE | ID: mdl-31756074

RESUMO

Antiepileptic drugs (AEDs) are the primary agents prescribed for clinical management of limbic epilepsy. However, high incidence of pharmacoresistance and a limited armory of drugs for inhibiting the pathological progression of epilepsy pose major obstacles to managing epilepsy. Here, we investigated the effect of Tetramethylpyrazine (TMP), the main bioactive alkaloid isolated from the oriental medicine Ligusticum chuanxiong Hort., against the epileptogenesis progression of acute hippocampal and corneal (6 Hz) electrical kindling models of TLE. TMP dose-dependently limited the progression of seizures and reduced the after-discharge duration (ADDs) in a hippocampal mouse kindling model. Mice treated with TMP (20, 50 mg/kg, i.p.) remained in stage 1 of epileptic progression for a protracted period, requiring additional stimulation to induce stages 2-5 epileptic phenotypes. TMP (50 mg/kg) also inhibited 6 Hz corneal kindling progression. In contrast, TMP did not reverse the phenotypes induced in a generalized seizures (GS) model, or the maximal electroshock (MES) or pentylenetetrazole (PTZ)-induced models of epilepsy. Furthermore, patch clamp recordings revealed no effect of TMP (10 µM) on CA1 hippocampal neurons' intrinsic properties but suppressed the (i) frequency of spontaneous excitatory post synaptic currents (sEPSCs), (ii) paired pulse ratio (PPR), and (iii) long-term potentiation (LTP) induction in the Schaffer collateral-CA1 pathway. TMP suppressed the activity of calcium, but not sodium, channels. Taken together, these results suggest that TMP has an anti-epileptogenic effect, likely through suppression of excitatory synaptic transmission by its effects on inhibition of calcium channels; these traits distinguish TMP from currently available AEDs. As mice administered TMP did not show any neurologic impairment in the object recognition and open field tests, the data support further development of TMP as a promising treatment for epilepsy.

7.
Pathol Res Pract ; 215(12): 152688, 2019 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-31727503

RESUMO

Osteosarcoma (OS) is known as a tumor that derives from skeletal system with increasing incidence worldwide. This study aimed to explore the effect of a circular RNA (circRNA), circ_0102049, on OS and reveal its potential molecular mechanism. In this work, the expression of circ_0102049 was detected by quantitative real time polymerase chain reaction (qRT-RCR) in both OS specimens and cell lines. The relationship between circ_0102049 level and patients' overall survival was evaluated by Kaplan-Meier curves and Cox regression analysis. Cell proliferation, apoptosis, migration and invasion of U2OS and MG63 cells were measured by cell counting kit-8 (CCK-8), flow cytometry, wound healing and transwell experiments, respectively. In addition, subcellular fractionation, bioinformatics analysis and dual-luciferase reporter assay were utilized to reveal the mechanism of circ_0102049 in OS. Circ_0102049 was overexpressed in both OS specimens and cells. Moreover, the level of circ_0102049 in OS patients was markedly correlated with larger tumor size, pulmonary metastasis and poor prognosis. Circ_0102049 remarkably accelerated cell proliferation, migration and invasion but attenuated cell apoptosis in OS cells analyzed by gain/loss of function experiments. What's more, we identified that circ_0102049 functions as a competing endogenous RNA (ceRNA) to competitively sponge miR-1304-5p to upregulate MDM2 expression at posttranscriptional level, thus mediating the cellular behaviors of OS cells. Collectively, our study provides an innovatively regulatory mechanism of circ_0102049 in OS and points out a new way for OS treatment.

8.
Tumori ; : 300891619886656, 2019 Nov 18.
Artigo em Inglês | MEDLINE | ID: mdl-31736439

RESUMO

PURPOSE: To prospectively investigate changes in quantitative parameters of dynamic contrast-enhanced magnetic resonance imaging (DCE-MRI) and the apparent diffusion coefficient (ADC) of diffusion-weighted imaging (DWI) in patients with cervical cancer before and after neoadjuvant chemotherapy (NACT). METHODS: Thirty-eight patients with cervical cancer underwent DCE-MRI and DWI 1 week before and 4 weeks after NACT. The patients were classified into 2 groups: significant reaction (sCR) group and the non-sCR group. DCE-MRI parameters and ADC values were measured and compared between the 2 groups. RESULTS: Before NACT, the mean Ktrans value was higher, but the mean Ve was lower, in the sCR group compared with the non-sCR group; these differences were statistically significant (p<0.05). After NACT, the mean Ktrans value and the delta (i.e., changed) value of Ktrans were significantly lower in the sCR group compared with the non-sCR group (p<0.05). However, the mean ADC and the delta value of the mean ADC between the 2 groups were slightly higher in the sCR group compared with the non-sCR group (p<0.05). The area under the curve of pre-mean Ktrans, DKtrans, and pre-mean Ktrans combined with post-mean ADC values were 0.801, 0.955, and 0.878, respectively (p<0.05). The optimal cutoff values for distinguishing sCR from non-sCR were pretreatment Ktrans (0.7020 min-1) and DKtrans (0.0437 min-1). CONCLUSIONS: Quantitative parameters (pre-mean Ktrans, DKtrans, and pre-mean Ktrans) combined with post-mean ADC could predict treatment efficacy more precisely. However, quantitative DCE-MRI combined with DWI could not significantly improve prognostic efficacy.

9.
J Phys Chem A ; 2019 Nov 25.
Artigo em Inglês | MEDLINE | ID: mdl-31765568

RESUMO

The conformations and molecular structures of diisopropyl disulfide have been studied by high resolution microwave spectroscopy and quantum chemical calculations. Three conformers, G'GG', G'GT and GGG', have been observed in the jet expansion. The global minimum, G'GG', adopts a configuration with the G' orientation of the H-C-S-S and S-S-C-H and the G orientation of the C-S-S-C showing the C2 symmetry. The rotational spectra of mono-substituted 13C and 34S isotopologues have also been measured for G'GG' leading to an accurate structural determination of this conformer. Two additional 34S isotopologues have also been measured for G'GT. The relative energies of three observed conformers calculated at the MP2/6-311++(d,p) level of theory are within 2 kJmol-1, while the relative intensity measurements suggested their population ratio to be NG'GG'/NG'GT/NGGG' ≈ 5:3:2.

10.
J Chem Phys ; 151(16): 164307, 2019 Oct 28.
Artigo em Inglês | MEDLINE | ID: mdl-31675896

RESUMO

The rotational spectrum of 2-thiophenecarboxaldehyde was investigated by using supersonic jet Fourier transform microwave spectroscopy. The measurements were extended to the 34S, 33S, 13C, and 18O isotopologs for the cis conformer, as well as to the 34S and 13C isotopologs for the trans conformer, leading to an accurately structural determination of the two observed conformers. The unchanged experimental Pcc values upon isotopic substitution indicate effective planar geometries of the two conformers. The ring structures of thiophene are slightly different between the cis and trans conformers. Two isomers of the monohydrated complex of 2-thiophenecarboxaldehyde, formed between a cis or trans monomer with water stabilized by an O-H⋯O hydrogen bond (HB) and an additional (C=O)CH⋯O(H2O) or (Cring)CH⋯O(H2O) HB, respectively, were observed in jet expansion. The noncovalent interactions attributed to the stabilization of the monomer and the monohydrated complex are revealed by quantum chemical methods. The interaction energy for trans-w-1 is 4 kJ mol-1 larger than that of cis-w-1, attributed to the relative stronger CH⋯O HB. The relative abundance of the two conformers of the 2-thiophenecarboxaldehyde monomer and the two isomers of the complex was estimated in the jet.

11.
Analyst ; 2019 Nov 21.
Artigo em Inglês | MEDLINE | ID: mdl-31750449

RESUMO

Different from the classical antiparallel DNA double-stranded structure, parallel DNA duplexes possess unique structures and potential biological functions. In this work, we found that the parallel DNA homoduplex from the [TG(GA)3]n sequence ([TG(GA)3]n-dsDNA) can dramatically enhance the fluorescence of thioflavin T (ThT), and the fluorescence enhancement is proportional to the number (n) of TG(GA)3 units in [TG(GA)3]n. Compared with the traditional G-quadruplex/ThT system, [TG(GA)3]n/ThT showed more stable and stronger fluorescence emission. In addition, coupled with an isothermal exponential amplification reaction, [TG(GA)3]3/ThT was used as a label-free fluorescent probe to detect microRNA, and the [TG(GA)3]3/ThT probe exhibited higher sensitivity than the G-quadruplex/ThT probe. This work provides a new paradigm to design label-free fluorescent biosensing/imaging systems.

12.
J Agric Food Chem ; 2019 Nov 15.
Artigo em Inglês | MEDLINE | ID: mdl-31690068

RESUMO

Dietary supplementation with conjugated linoleic acid (CLA) has been reported to alleviate the effect of colitis in mice, but the mechanisms involved need further exploration. The study aimed to investigate how orally administered CLA alleviates dextran sulfate sodium (DSS)-induced colitis in mice. CLA was administered in five different doses: 40, 20, 10, 5, and 2.5 mg/day. Doses of CLA at 10 mg/day and higher alleviated colitis symptoms and reduced inflammation induced by DSS, in which 40, 20, and 10 mg/day CLA significantly increased the concentration of mucin2 and goblet cells, but neither 5 mg/day CLA nor 2.5 mg/day CLA had any effects. Meanwhile, 40 and 20 mg/day CLA treatments significantly upregulated the concentration of tight junction proteins (ZO-1, occludin, and claudin-3) and ameliorated epithelial apoptosis caused by DSS. Moreover, oxidative-stress-related enzymes (superoxide dismutase, glutathione peroxidase, and catalase) and inflammatory cytokines [tumor necrosis factor-α, interleukin (IL)-10, and IL-6] were modulated by 40 and 20 mg/day CLA. Furthermore, 40 mg/day CLA rebalanced the gut microbiota damaged by DSS, including reducing Bacteroides and increasing Bifidobacterium and Odoribacter. In conclusion, CLA supplementation alleviated DSS-induced colitis in a dose-dependent manner by modulating inflammatory cytokines and oxidation stress, maintaining the mucosal barrier, and reverting microbiota changes.

13.
Sci Total Environ ; 703: 134805, 2019 Oct 31.
Artigo em Inglês | MEDLINE | ID: mdl-31733499

RESUMO

Microplastics (MPs), polymer particles capable of adsorbing heavy metals from ambient environment, have been found in diverse human food resources. Through the consumption of MPs, heavy metals adsorbed on MPs might be transported into human body. This study aims to explore the behavior of heavy metal-contaminated MPs in human digestive system which is not previously researched. Firstly, a chromium (Cr) adsorption/desorption study was conducted with four commonly used nondegradable MPs [polyethylene (PE), polypropylene (PP), polyvinyl chloride (PVC) and polystyrene (PS)] as well as one degradable MP (polylactic, PLA). Then, the whole digestive system in-vitro method (WDSM), a systematic model including mouth, gastric, small intestine, and large intestine digestive phases, was conducted on the Cr-loaded MPs. Additionally, the bioaccessibilities and hazard quotients (HQs) of Cr(VI) and Cr(III) were evaluated. Among five MPs, although PLA showed the weakest adsorption capacity for Cr, the Cr(VI) bioaccessibilities for PLA reached the highest values of 19.9%, 15.6% and 3.9% in gastric, small intestinal and large intestinal phases, respectively. The bioaccessibilities of Cr(VI) in gastric phase were significantly higher than those in other phases, while no Cr release from MPs was detected in the mouth phase. In gastric phase, the bioaccessibilities of Cr(VI) were significantly higher than those of Cr(III) in the gastric phase, and both of them approached to a similar level in intestinal phases. In the WDSM, the HQs of Cr(VI) and Cr(III) on MPs were lower than the critical level for both adults and children. Based on the measured bioaccessibilities, the maximum daily total Cr intake for different human groups (female children, male children, female adults and male adults) through MP consumption was estimated from 0.50 to 1.18 µg/day. In general, the five tested MPs were potential to serve as Cr vectors in the WDSM.

15.
Zhongguo Zhong Yao Za Zhi ; 44(17): 3608-3614, 2019 Sep.
Artigo em Chinês | MEDLINE | ID: mdl-31602930

RESUMO

In order to provide scientific basics for exploitation and sufficient application of Polyporus umbellatus resources and study the monosaccharide composition of P. umbellatus polysaccharides,the anthrone-sulfuric acid method was applied to compare polysaccharide content of P. umbellatus from 17 producing areas. The monosaccharides were derived by 1-phenyl-3-methyl-5-pyrazolone( PMP) and the derivatives were identified by UPLC-MS/MS and the content of each monosaccharide component was determined simultaneously. The results demonstrated that there was a certain difference in total polysaccharide content of P. umbellatus from different regions,and the content of total P. umbellatus polysaccharide from Shaanxi province and Sichuan province( 1. 15% and 1. 90%) was relatively higher than that of others areas. Polysaccharides from P. umbellatus was mainly composed of eight monosaccharides,including glucose,glucuronic acid,galactose,ribose,xylose,arabinose,mannose and fucose. The contents of glucose( 17. 65 mg·g-1) was higher than others. The ribose was the lowest( 0. 13 mg·g-1). In addition,fructose,rhamnose and galacturonic acid were also detected in some samples. Furthermore,the results of cluster analysis( CA) and principal component analysis( PCA) indicated that totally 17 batches of P. umbellatus polysaccharide could be classified into three clusters,samples collected from Wuchang in Heilongjiang province were clustered into one group separately. The study can provide a basis for rational utilization of P. umbellatus resources,and also implies the sequence of monosaccharide linking and pharmacological activity of P. umbellatus polysaccharides.


Assuntos
Monossacarídeos/química , Polyporus/química , Polissacarídeos/química , China , Cromatografia Líquida de Alta Pressão , Geografia , Espectrometria de Massas em Tandem
16.
Zhongguo Zhong Yao Za Zhi ; 44(17): 3633-3636, 2019 Sep.
Artigo em Chinês | MEDLINE | ID: mdl-31602934

RESUMO

As a treasure of traditional Chinese medicine culture,Dao-di herbs are famous for their high quality and good effect.However,traditional characteristics of Dao-di herbs and their producing areas are mostly confined to qualitative description,lacking objective evaluation indicators. Numerical taxonomy,which uses mathematical methods to improve the research of taxonomy from qualitative description to quantitative comprehensive analysis,is objective and accurate,so is widely used in the field of biology and medicine.This paper reviews the concept and main research fields of numerical taxonomy,and summarizes its application status and development prospects in the study of Dao-di herbs and characteristics of production areas,in order to lay the foundation for the objectification and standardization of Dao-di herbs.


Assuntos
Medicamentos de Ervas Chinesas/classificação , Plantas Medicinais/classificação , Medicamentos de Ervas Chinesas/normas , Medicina Tradicional Chinesa
17.
Pain ; 2019 Oct 18.
Artigo em Inglês | MEDLINE | ID: mdl-31651582

RESUMO

Chronic pain and anxiety symptoms are frequently encountered clinically, but the neural circuit mechanisms underlying the comorbid anxiety symptoms in pain (CASP) in context of chronic pain remain unclear. Using viral neuronal tracing in mice, we identified a previously unknown pathway whereby glutamatergic neurons from layer 5 of the hindlimb primary somatosensory cortex (S1) (Glu), a well-known brain region involved in pain processing, project to GABAergic neurons in the caudal dorsolateral striatum (GABA). In a persistent inflammatory pain model induced by complete Freund's adjuvant injection, enhanced excitation of the Glu→GABA pathway was found in mice exhibiting CASP. Reversing this pathway using chemogenetic or optogenetic approaches alleviated CASP. In addition, the optical activation of Glu terminals in the cDLS produced anxiety-like behaviors in naive mice. Overall, the current study demonstrates the putative importance of a novel Glu→GABA pathway in controlling at least some aspects of CASP.

18.
Proc Natl Acad Sci U S A ; 116(46): 23264-23273, 2019 Nov 12.
Artigo em Inglês | MEDLINE | ID: mdl-31662475

RESUMO

Glycolytic enzyme phosphoglycerate mutase 1 (PGAM1) plays a critical role in cancer metabolism by coordinating glycolysis and biosynthesis. A well-validated PGAM1 inhibitor, however, has not been reported for treating pancreatic ductal adenocarcinoma (PDAC), which is one of the deadliest malignancies worldwide. By uncovering the elevated PGAM1 expressions were statistically related to worse prognosis of PDAC in a cohort of 50 patients, we developed a series of allosteric PGAM1 inhibitors by structure-guided optimization. The compound KH3 significantly suppressed proliferation of various PDAC cells by down-regulating the levels of glycolysis and mitochondrial respiration in correlation with PGAM1 expression. Similar to PGAM1 depletion, KH3 dramatically hampered the canonic pathways highly involved in cancer metabolism and development. Additionally, we observed the shared expression profiles of several signature pathways at 12 h after treatment in multiple PDAC primary cells of which the matched patient-derived xenograft (PDX) models responded similarly to KH3 in the 2 wk treatment. The better responses to KH3 in PDXs were associated with higher expression of PGAM1 and longer/stronger suppressions of cancer metabolic pathways. Taken together, our findings demonstrate a strategy of targeting cancer metabolism by PGAM1 inhibition in PDAC. Also, this work provided "proof of concept" for the potential application of metabolic treatment in clinical practice.

19.
Biosens Bioelectron ; 145: 111730, 2019 Dec 01.
Artigo em Inglês | MEDLINE | ID: mdl-31590074

RESUMO

Single-cell analysis is critical to understanding its heterogeneity and biological processes, such as stem cell differentiation, and elucidating the underlying mechanisms of cellular metabolism. New tools to promote intercellular variability studies help elucidate cellular regulation mechanisms. Here an impedance measurement and analysis system was built to monitor the osteogenic differentiation of single bone marrow mesenchymal stem cells (BM-MSCs) in droplets. The biochip including a microelectrode array was designed based on droplet microfluidics and fabricated. A novel theoretical electrical model was proposed to simulate the electrical properties of cells in the droplets. Impedance measurements showed that single cells are substantially heterogeneous during osteoblast differentiation at different stages (days 0, 7, 14 and 21) and different cell passages (passages 6, 7 and 11). This result was consistent with the appearance of two biomarkers (alkaline phosphatase and calcium nodules), which are the gold standard biomarkers of osteoblastogenesis and differentiation. The device enabled highly efficient single-cell trapping, accurate positioning, and sensitive, label-free and noninvasive impedance measurements of individual cells with multiple channels. This system provides a strategy for exploring the processes of osteoblastogenesis and differentiation at the single-cell level and has substantial potential for applications in the biomedical field.

20.
Artigo em Inglês | MEDLINE | ID: mdl-31658050

RESUMO

OBJECTIVES: To identify the associated factors for malignancy in partially cystic thyroid nodules (PCTNs) on ultrasound (US). MATERIALS AND METHODS: This is a retrospective study. 338 PCTNs confirmed by surgery was included in this study. The US features of the nodules were reviewed and their significance in differential diagnosis was analyzed. RESULTS: In the 338 PCTNs, 50 were malignant and 288 were benign. Univariate analyses revealed that a taller-than-wide shape and spiculated or microlobulated margin were significantly associated with malignancy while the spongy form, an ovoid to round shape and smooth margin were significantly associated with benign nature. In terms of the internal solid portion of the nodule, eccentric configuration, non-smooth margin, hypoechogenicity, and microcalcification were more frequently demonstrated in malignant nodules than in benign ones. In the multivariate logistic regression analysis, a taller than wide shape (OR: 13.357; 95% CIs: 3.457-28.797) was revealed to be the strongest independent predictor for malignancy, followed by microcalcification (OR: 11.578; 95% CIs: 3.142-29.563), spiculated or microlobulated margin (OR: 6.331; 95% CIs: 1.425-9.457), eccentric configuration (OR: 5.456; 95% CIs: 1.358-8.774), non-smooth rim of the internal solid portion (OR: 4.897; 95% CIs: 0.754-7.021), and hypoechogenicity of the internal solid portion (OR: 4.651; 95% CIs: 0.712-8.245). CONCLUSIONS: Understanding the ultrasound characteristics of malignant PCTNs is important to make a precise diagnosis of thyroid nodules.

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