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Zhongguo Zhong Yao Za Zhi ; 38(16): 2577-80, 2013 Aug.
Artigo em Chinês | MEDLINE | ID: mdl-24228565

RESUMO

OBJECTIVE: To establish sequence characterized amplified region markers of Polygonum capitatum. METHOD: The random primer was screened through RAPD to obtain the specific RAPD marker band, and the band was separated, extracted, cloned and sequenced. The specific primers were designed for conventional PCR reaction on the basis of the specific band, and the SCAR marker was acquired. RESULT: Screening from 50 RAPD primer, only C29 primer had 2 specific bands could distinguish P. capitatum from P. nepalense, then 4 pairs of specific primers were designed based on the 2 sequences of RAPD marker bands, and only 1 pair primer (Z1-2) was successfully converted into SCAR marker after repeated tests. CONCLUSION: The Z1-2 primer, could be used as an effective SCAR mark to identify Z300 DNA for P. capitatum. The SCAR mark was established and can be used as a molecular marker to distinguish P. capitatum from P. nepalense


Assuntos
Polygonum/classificação , Polygonum/genética , Técnica de Amplificação ao Acaso de DNA Polimórfico , Análise de Sequência de DNA , Código de Barras de DNA Taxonômico , DNA de Plantas/genética , Marcadores Genéticos/genética
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