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1.
Environ Toxicol Pharmacol ; : 103381, 2020 May 12.
Artigo em Inglês | MEDLINE | ID: mdl-32413495

RESUMO

To investigate heavy metal exposure in an industrial zone vs. a living quarter in Shanghai and explore the relationship between the heavy metal source and urine cadmium (Cd) and early kidney damage. Blood lead and urine Cd, manganese (Mn), mercury (Hg), arsenic (As) and EKD indexes were compared between residents in Exposure group (n = 168) and Control group (n = 168). It was found that PM2.5 level in Exposure group was significantly higher than that in Control group, and serum Cys-C and urine Cd, NAG, mAlb, KIM-1 and Cd-MT levels in Exposure group were also significantly higher than those in Control group, suggesting that differences in urine Cd and heavy metal levels between the residents of the two groups may be due to different PM2.5 concentrations in the environments of the two areas. Cd accumulation within the human body can induce kidney damage, probably through its potential hazard to the proximal tubular epithelial cells.

2.
Spectrochim Acta A Mol Biomol Spectrosc ; 237: 118301, 2020 Apr 13.
Artigo em Inglês | MEDLINE | ID: mdl-32375078

RESUMO

High-level ab initio computations have been performed on the experimentally unknown species SCl+. The low-lying Λ-S electronic states correlated to the first and the second dissociation channels as well as their corresponding Ω states have been investigated by the icMRCI+Q methodology employing basis sets up to quintuple-ζ quality. Information about potential energy curves, electron configurations, spectroscopic constants, dipole moments and transition properties are derived and discussed. The results for SCl+ represent an improvement over our previous theoretical descriptions for the ground state. In addition, several low-lying excited states that have not been accessed experimentally and theoretically are also been well characterized in this work. The accuracy of our predictions for SCl+ are verified by comparisons of spectroscopic constants and vibrational levels between our accompany SCl computations and those reported in literatures for the neutral species. The feasibility of performing laser cooling of SCl+ has also been discussed and the photoelectron spectrum of SCl+(X3Σ-) + e â† SCl(X2Π) is simulated.

3.
Cell Mol Biol (Noisy-le-grand) ; 66(2): 87-92, 2020 May 15.
Artigo em Inglês | MEDLINE | ID: mdl-32415932

RESUMO

Cerebral infarction is an acute cerebrovascular disease caused by abnormal blood circulation in the brain. In the present study, we investigate the effect of astragaloside IV on cognitive dysfunction in cerebrally infarcted rats via transforming growth factor-ß (TGF-ß) / Smad signaling pathway. For this purpose, 45 rats were divided into three groups including astragaloside, model, and control. 30 of 45 healthy adult male SD rats were randomly selected to establish an acute cerebral infarction model. 15 modeled rats were enrolled as a model and astragaloside group, and another 15 rats as a blank control group. The rats in the astragaloside group were fed with astragaloside IV according to 1.08 g/kg body weight, and those in the blank group and model group were given matching normal saline. The levels of TGF-ß, Smad1, Smad3 and Smad7 of TGF-ß/Smad signaling transduction pathway at T0 (week 0), T1 (week 3) and T2 (week 6) were determined by enzyme-linked immunosorbent assay (ELISA). The modified neurological severity score (mNSS) was used to evaluate the improvement of cognitive dysfunction in rats. The mNSS of rats with cerebral infarction in the astragaloside group was lower than that in the control group and model group (P< 0.05). While the levels of TGF-ß, Smad1, Smad3 and Smad7 in the astragaloside group were higher than those in the control group and model group (P< 0.05). Astragaloside IV plays an important role in improving cognitive dysfunction in rats with cerebral infarction while affecting the levels of TGF-ß, Smad1, Smad3 and Smad7 and activating TGF-ß / Smad signaling pathway.

4.
Leuk Lymphoma ; : 1-8, 2020 Apr 23.
Artigo em Inglês | MEDLINE | ID: mdl-32324088

RESUMO

The T lymphocyte system plays an active role in tumor immunosurveillance in multiple myeloma (MM), and abnormal T lymphocyte populations are often observed in patients with MM. In the current study, we evaluated the prognostic impact of abnormal T lymphocyte subset distributions in patients with newly diagnosed MM (NDMM). Between December 2012 to October 2016, 110 NDMM patients were included in this study. Multiparameter flow cytometry was applied to quantitatively analysis the peripheral blood T lymphocyte subsets, including CD4+ T cell, CD8+ T cell, and CD4/CD8 ratio. Survival analyses were performed based on the patients' clinical data and the quantity of T lymphocyte subsets. The median follow-up time was 27.0 months (range, 2.5-66). Baseline percentages and absolute CD4+ T cell counts and the CD4/CD8 ratio were positively correlated with both overall survival (OS) and progression-free survival (PFS) according to Kaplan-Meier curves (p < .05). In the multivariate COX analysis, lower CD4+ T cell count was an independent unfavorable factor in predicting both OS (p = .016) and PFS (p = .010). Furthermore, lower CD4/CD8 ratio was an independent adverse prognostic factor for shorter PFS (p = .017). These results suggested that T lymphocyte subsets were crucial indicators in correlation with MM patients' prognosis. Low CD4+ T cell counts and CD4/CD8 ratio were independent unfavorable prognostic predictors for patients with MM at diagnosis.

5.
J Gerontol Nurs ; 46(5): 23-30, 2020 May 01.
Artigo em Inglês | MEDLINE | ID: mdl-32324892

RESUMO

Hospitalized older adults with cognitive impairment (CI) due to dementia and/or delirium may require individualized care strategies such as direct observation to mitigate safety concerns and manage behavioral symptoms. Despite common use of direct observation as a strategy, little is known about its practice and outcomes. A systematic review was conducted to identify, appraise, and synthesize literature on direct observation among hospitalized older adults with CI. The search yielded 16 eligible studies, with four describing current practices, nine reporting quality improvement efforts to broaden direct observation, and three focusing on direct observation reduction. Strength of evidence across studies was weak, limited in scope, and lacking clarity in definitions, indications for use and discontinuation, and documentation. Overall, findings highlight differing views on direct observation and the need for future, rigorous evaluation of approaches (e.g., nursing autonomy in initiating and discontinuing observation) to better align direct observation with patient needs. [Journal of Gerontological Nursing, 46(5), 23-30.].

6.
Artigo em Inglês | MEDLINE | ID: mdl-32103663

RESUMO

Growth of large-area, uniform, and high-quality monolayer transition-metal dichalcogenides (TMDs) for practical and industrial applications remains a long-standing challenge. The present study demonstrates a modified predeposited chemical vapor deposition (CVD) process by employing an annealing procedure before sulfurization, which helps in achieving large-area, highly uniform, and high-quality TMDs on various substrates. The annealing procedure resulted in a molten liquid state of the precursors in the CVD process, which not only facilitated a uniform redistribution of the precursor on the substrate (avoid the aggregation) because of the uniform redistribution of the liquid precursor on the substrate but more importantly avoided the undesired multilayer growth via the self-limited lateral supply precursors mechanism. A 2 in. uniform and continuous monolayer WS2 film has been synthesized on the SiO2/Si substrate. Moreover, uniform monolayer WS2 single crystals can be prepared on more general and various substrates including sapphire, mica, quartz, and Si3N4 using the same growth procedure. Besides, this growth mechanism can be generalized to synthesize other monolayer TMDs such as MoS2 and MoS2/WS2 heterostructures. Hence, the present method provides a generalized attractive strategy to grow large-area, uniform, single-layer two-dimensional (2D) materials. This study has significant implications in the advancement of batch production of various 2D-material-based devices for industrial and commercial applications.

7.
Spectrochim Acta A Mol Biomol Spectrosc ; 227: 117667, 2020 Feb 15.
Artigo em Inglês | MEDLINE | ID: mdl-31698155

RESUMO

High-level ab initio computations have been performed on SnH+. The potential energy curves and spectroscopic constants of the low-lying Λ-S electronic states, as well as their associated Ω states, are derived at the icMRCI + Q level employing basis sets of quintuple-ζ quality. The transition dipole moments, Einstein coefficients, radiative lifetimes and Franck-Condon factors of three spin-forbidden transition bands ( [Formula: see text] , [Formula: see text] and [Formula: see text] ) are determined. Comparisons between our predictions and available experimental results indicate reasonable agreement. The spin-orbit coupling effect has been proved to affect these low-lying electronic states significantly.

8.
Cancer Chemother Pharmacol ; 85(1): 185-193, 2020 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-31745591

RESUMO

PURPOSE: The human peroxiredoxin-5 (hPRDX5) is a member of the family of antioxidant enzymes, which could resist immunosuppression by promoting immune organs development, lymphocyte proliferation and up-regulation of the levels of serum cytokines. However, being a recombinant protein, the hPRDX5 exhibits some problems including the high production cost and bad tissue penetration. Compared to macromolecular therapeutic agents, synthetic peptides have several advantages as drug candidates, such as lower manufacturing costs, reduced immunogenicity, and better organ or tumor penetration. The purpose of this research was to design the novel peptides come from hPRDX5 that can block the interaction of PD-1 and PD-L1. METHODS: Herein in this work, we firstly confirmed the inhibitory activity of hPRDX5 on the binding of PD-L1 to PD-1 based on the previous observation, subsequently, in silico proteolysis and rational design (such as alanine scanning mutagenesis and truncation) were used to automate the design of new peptides derived from hPRDX5 with anti-tumour activity. RESULTS: We found that the most potent peptide could block the PD-1/PD-L1 interaction effectively with an IC50 of 0.646 µM, and could restore the function of Jurkat T cells which had been suppressed by stimulated HCT116 cells. Moreover, experiments with tumor-bearing mice models showed that the peptide IMB-P6-10 could effectively inhibit tumor growth and showed extraordinary low acute toxicity in vivo. CONCLUSIONS: The peptides described in this paper may provide novel low-molecular-weight drug candidates for cancer immunotherapy.

9.
Viral Immunol ; 2019 Dec 13.
Artigo em Inglês | MEDLINE | ID: mdl-31834847

RESUMO

The prevalence of hepatitis B virus (HBV) infection was an imbalance in different provinces of China. This study aimed to investigate the prevalence of HBV infection and evaluate the prophylactic measures in a public hospital of northeast China over the preceding 13 years. A total of 13,948 patients in 2004 and 15,256 patients in 2017 of Shengjing Hospital of China Medical University were tested of serum HBsAg, HBeAg, HBsAb, HBeAb, and HBcAb levels with Abbott MEIA Kits. In people born before 1992, HBsAg-positive rate was 5.45% and 6.47%; isolated HBsAb positive rate was 14.62% and 21.24%; HBV marker negative rate was 54.27% and 42.77% in 2004 and 2017 survey, respectively. The males had a significant higher HBsAg-positive rate than the females. In people born during 1992-2004, HBsAg positive rate was 0.58% and 0.57%, isolated HBsAb positive rate was 41.47% and 46.57%; and HBV marker negative rate was 51.97% and 46.86% in 2004 and 2017 survey, respectively. Males and females had no difference of HBsAg-positive rate. In children born after 2005, HBsAg positive rate was 0.11%, isolated HBsAb positive rate was 76.68%, and HBV marker negative rate was 18.51% in 2017 survey. No difference of HBsAg-positive rate was found between the genders. A dramatic decrease of HBsAg positive rate and a progressive increase of HBsAb-positive rate were found among people born after 1992 and progressed further in those born after 2005. Immunization of infants and timely birth dose was the key method for prevention of HBV infection. Expanded HB vaccination would be needed for people born before 2005, especially those born between 1992 and 2004.

10.
Phys Chem Chem Phys ; 21(39): 21746-21752, 2019 Oct 09.
Artigo em Inglês | MEDLINE | ID: mdl-31552405

RESUMO

A theoretical study of geometrical structures and electronic properties of niobium-doped boron clusters is performed using the CALYPSO approach for the global minimum search followed by density functional theory calculations. It is found that the global minima obtained for the neutral clusters correspond to the half-sandwich structures at n = 10-17 and the tubular-type structures at n = 18-20. The geometrical patterns in the anion series are more complex. The geometries undergo a transformation from the wheel-like structure of NbB10- to the half-sandwich ones beginning at n = 11 and finally to the drum-shaped structures at n ≥ 18. A fascinating NbB12- cluster is uncovered by our structural search, which shows robust stability and can be considered as a new member of the half-sandwich transition metal doped boron clusters. The chemical bond analysis indicates that the high stability is due to the strong interactions between the Nb atom and the B12 host as well as to the strong B-B covalent bonds. Our study will enrich the database of geometrical structures of transition metal doped boron clusters and will stimulate future synthesis of boron-based nanomaterials.

11.
ACS Nano ; 13(7): 8265-8274, 2019 Jul 23.
Artigo em Inglês | MEDLINE | ID: mdl-31283181

RESUMO

Shape engineering plays a crucial role in the application of two-dimensional (2D) layered metal dichalcogenide (LMD) crystalline materials in terms of physical and chemical property modulation. However, controllable growth of 1T phase tin disulfide (SnS2) with multifarious morphologies has rarely been reported and remains challenging. Herein, we report a direct synthesis of large-size, uniform, and atomically thin 1T-SnS2 with multiple morphologies by adding potassium halides via a facile chemical vapor deposition process. A variety of morphologies, i.e., from hexagon, triangle, windmill, and dendritic to coralloid, corresponding to fractal dimensions from 1.01 to 1.81 are accurately controlled by growth conditions. Moreover, the Sn concentration controls the morphology change of SnS2. The edge length of the SnS2 dendritic flake can grow larger than 500 µm in 5 min. Potassium halides can significantly reduce the surface migration barrier of the SnS2 cluster and enhance the SnS2 adhesion force with substrate to facilitate efficient high in-plane growth of monolayer SnS2 compared to sodium halides by density functional theory calculations. More branched SnS2 with higher fractal dimension provides more active sites for enhancing hydrogen evolution reactions. Importantly, we prove that potassium halides are preferable for 1T-phase LMDs structures, while sodium halides are more suitable for 2H-phase materials. The growth mechanism proposed here provides a general approach for controllable-phase synthesis of 2D LMD crystals and related heterostructures. Shape engineering of 2D materials also provides a strategy to tune LMD properties for demanding applications.

12.
Mol Imaging Biol ; 2019 Jun 18.
Artigo em Inglês | MEDLINE | ID: mdl-31292914

RESUMO

PURPOSE: Scintigraphic imaging of malignant glioblastoma (MG) continues to be challenging. We hypothesized that VPAC1 cell surface receptors can be targeted for positron emission tomography (PET) imaging of orthotopically implanted MG in a mouse model, using a VPAC1-specific peptide [64Cu]TP3805. PROCEDURES: The expression of VPAC1 in mouse GL261 and human U87 glioma cell lines was determined by western blot. The ability of [64Cu]TP3805 to bind to GL261 and U87 cells was studied by cell-binding. Receptor-blocking studies were performed to validate receptor specificity. GL261 tumors were implanted orthotopically in syngeneic T-bet knockout C57BL/6 mouse brain (N = 15) and allowed to grow for 2-3 weeks. Mice were injected i.v., first with ~ 150 µCi of 2-deoxy-2-[18F]fluoro-D-glucose ([18F]FDG) then 24 h later with ~ 200 µCi of [64Cu]TP3805. In another set of tumor-bearing mice, (N = 5), ionic [64Cu]Cl2 was injected as a control. Mice were imaged at a 2-h post-injection using an Inveon micro-PET/CT, sacrificed and % ID/g of [64Cu]TP3805 and [64Cu]Cl2 were calculated in a tumor, normal brain, and other tissues. For histologic tissue examination, 3-µm thick sections of the tumors and normal brain were prepared, digital autoradiography (DAR) was performed, and then the sections were H&E stained for histologic examination. RESULTS: Western blots showed a strong signal for VPAC1 on both cell lines. [64Cu]TP3805 cell-binding was 87 ± 1.5 %. Receptor-blocking reduced cell-binding to 24.3 ± 1.5 % (P < 0.01). PET imaging revealed remarkable accumulation of [64Cu]TP3805 in GL261 MG with a negligible background in the normal brain, as compared to [18F]FDG. Micro-PET/CT image analyses and tissue distribution showed that the brain tumor uptake for [64Cu]TP3805 was 8.2 ± 1.7 % ID/g and for [64Cu]Cl2 2.1 ± 0.5 % ID/g as compared to 1.0 ± 0.3 % ID/g and 1.4 ± 0.3 % ID/g for normal mouse brains, respectively. The high tumor/normal brain ratio for [64Cu]TP3805 (8.1 ± 1.1) allowed tumors to be visualized unequivocally. Histology and [64Cu]TP3805 DAR differentiated malignant tumors from healthy brain and confirmed PET findings. CONCLUSION: Targeting VPAC1 receptors using [64Cu]TP3805 for PET imaging of MG is a promising novel approach and calls for further investigation.

13.
Medicine (Baltimore) ; 98(8): e13918, 2019 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-30813122

RESUMO

RATIONALE: Spasticity is a common issue in chronic stroke. To date, no study has reported the long-term (up to 1 year) outcomes of Fu's subcutaneous needling in combination with constraint-induced movement therapy in chronic stroke. This report describes the successful addition of acupuncture on spasticity and arm function in a patient with chronic stroke and arm paresis. PATIENT CONCERNS: The patient suffered an infarction in the right posterior limb of the internal capsule 1 year ago, which resulted in hemiparesis in his left (nondominant) hand and arm. The only limitation for constraint-induced movement therapy was insufficient finger extension. The patient was unable to voluntarily extend his interphalangeal or metacarpophalangeal joints beyond the 10 degrees required for constraint-induced movement therapy. However, his muscle tension did not change after the BTX type A injection. DIAGNOSES: A 35-year-old male experienced arm paresis after an infarction in the right posterior limb of the internal capsule 1 year before the intervention. INTERVENTIONS: The BTX type A injection did not work, so the patient received Fu's subcutaneous needling as an alternative therapy before 5 h of constraint-induced movement therapy for 12 weekdays. OUTCOMES: All outcome measures (Modified Ashworth Scale, Fugl-Meyer Assessment, Action Research Arm Test, and Motor Activity Log) substantially improved over the 1-year period. Moreover, during the observation period, the patient's muscle tone and arm function did not worsen. LESSONS: As a result of a reduction in spasticity, a reduction of learned nonuse behaviors, or use-dependent plasticity after the combined therapy, the arm functions include volitional movements, and coordination or speed of movements in the paretic arm have been improved. However, we cannot rule out the possibility of an influence of the passage of time or the Hawthorne effect. The costs of the treatment of stroke may be reduced, if this combined therapy proved useful in future controlled studies.


Assuntos
Terapia por Acupuntura/métodos , Técnicas de Exercício e de Movimento/métodos , Espasticidade Muscular/reabilitação , Paresia/reabilitação , Reabilitação do Acidente Vascular Cerebral/métodos , Terapia por Acupuntura/economia , Adulto , Braço/fisiopatologia , Doença Crônica , Terapia Combinada , Técnicas de Exercício e de Movimento/economia , Seguimentos , Humanos , Masculino , Espasticidade Muscular/etiologia , Agulhas , Paresia/etiologia , Acidente Vascular Cerebral/complicações , Resultado do Tratamento
14.
Phys Chem Chem Phys ; 21(13): 6935-6941, 2019 Mar 27.
Artigo em Inglês | MEDLINE | ID: mdl-30865191

RESUMO

To understand the effect of magnesium atom doping on boron clusters, here we report the lowest-lying energy structures and electronic properties of neutral and monoanionic magnesium-boron clusters, in which boron atoms range in size from 10 to 20, in the framework of a CALYPSO structural search and DFT calculations. One of the most stable combinations is MgB18, which adopts an unexpected tubular drum-shaped geometry that is found for the first time in boron clusters doped with alkaline-earth metal atoms. The stability of the MgB18 cluster mainly stems from the intense electrostatic interaction between the B18 skeleton and the Mg atom.

15.
Spectrochim Acta A Mol Biomol Spectrosc ; 208: 124-130, 2019 Feb 05.
Artigo em Inglês | MEDLINE | ID: mdl-30308396

RESUMO

The Λ-S electronic states with respect to the lowest four dissociation limits of BeSb are investigated theoretically on the icMRCI + Q level employing basis set of quintuple-ζ quality. The geometrical parameters, potential energy curves, vibrational energy levels, spectroscopic constants for the twelve Λ - S states are obtained, analyzed and compared with those of the Beryllium-VA group diatomic family species where data are available. The permanent dipole moments, transition dipole moments, Einstein emission coefficients, radiative lifetimes and Franck-Condon factors for interested Λ - S states are also derived. Further assessments of the spin-orbit coupling effect are performed for states associated with the first two dissociation asymptotes of BeSb. Four Λ - S states split into seven Ω states, and some of the PECs are distorted significantly through the spin-orbit coupling effect, which is similar to its isovalent diatomics BeAs. In consideration of potential risks of manipulating beryllium-containing species directly, the information associated with molecular structures, spectroscopic parameters as well as transition properties that provide in this paper is anticipated to serve as guidelines for further researches of BeSb.

16.
J Phys Condens Matter ; 31(11): 115401, 2019 Mar 20.
Artigo em Inglês | MEDLINE | ID: mdl-30557855

RESUMO

The iron-based compound BaFe2S3 has received considerable attention in recent years due to its unique electronic properties with important applications. But, there has been relatively little attention devoted to the structure evolution of BaFe2S3 under high pressure. Here, we report a detailed theoretical study of the structural, electronic and sound velocity properties of BaFe2S3 under high pressure by CALYPSO structure search method in combination with the first-principles calculations. We have uncovered three novel structures of BaFe2S3 under high pressure with space group P2221, Imm2, and C2/m symmetries. Theoretical calculations reveal that the structures of BaFe2S3 under high pressure satisfy the phase transition sequence of Cmcm → P2221 → Imm2 → C2/m, and the corresponding transition pressures are 31.6, 47.4 and 57.0 GPa, respectively. Our results enrich the high pressure phases of BaFe2S3 and will stimulate future experiments to synthesize these new phases.

17.
J Chromatogr A ; 1555: 37-44, 2018 Jun 22.
Artigo em Inglês | MEDLINE | ID: mdl-29728266

RESUMO

A solid-phase microextraction (SPME) fiber coated with a two-dimensional porous organic framework (POF), JUC-Z2, was synthesized by a sol-gel process. The JUC-Z2-coated SPME fiber was combined with gas chromatography-mass spectrometry/mass spectrometry (GC-MS/MS), and a direct analysis method without any derivatization step was developed for the determination of two major harmful aromatic amines (1-naphthylamine, 1-NA; 2-naphthylamine, 2-NA) in the urine of amokers. The new method exhibited satisfactory linearity in the wide range of 0.05-100 µg L-1 with correlation coefficients (R) of 0.9999. The limits of detection are 0.010 and 0.012 ng L-1. The relative standard deviations for repeated extraction (n = 6) with one fiber are less than 7.7%. The JUC-Z2-coated fiber demonstrates obviously superior extraction efficiencies for the two aromatic amines over the commercial fibers (CW/PEG, PA, PDMS/DVB, PDMS, CAR/PDMS, DVB/CAR PDMS). The structure-activity relationship was deeply investigated by the extraction comparison experiment with another similar POF material (PAF-48) and theoretical simulations. The extraction efficiencies of JUC-Z2 for 1-NA and 2-NA are 9.6 and 16.2 times higher than those of PAF-48, and JUC-Z2 has higher binding energies with the aromatic amines than PAF-48. The results indicate that the N atom on the JUZ-Z2 framework played a crucial role for adsorbing aromatic amines. Finally, the novel fiber has been successfully used to detect 1-NA and 2-NA in the urine of smokers.


Assuntos
Aminas/urina , Estruturas Metalorgânicas/química , Fumantes , Microextração em Fase Sólida/métodos , Cromatografia Gasosa-Espectrometria de Massas , Humanos , Limite de Detecção , Porosidade , Relação Estrutura-Atividade , Espectrometria de Massas em Tandem
18.
Spectrochim Acta A Mol Biomol Spectrosc ; 202: 368-375, 2018 Sep 05.
Artigo em Inglês | MEDLINE | ID: mdl-29803975

RESUMO

Theoretical investigations for NaMg have been performed on the icMRCI + Q level employing basis set of quintuple-ζ quality with corrections of core-valence correlation and scalar relativistic effect. The geometrical parameters, potential energy curves, vibrational energy levels, spectroscopic constants for the eight Λ-S states, with respect to the lowest four dissociation limits, are investigated. Through the spin-orbit coupling effect, these states split into fourteen Ω states. The permanent dipole moments, transition dipole moments, Einstein emission coefficients, radiative lifetimes and Franck-Condon factors for all Ω states are studied. The feasibility of performing laser cooling of NaMg has also been discussed. Our predictive results are anticipated to serve as guidelines for further researches on NaMg.

19.
Immunol Lett ; 198: 66-73, 2018 06.
Artigo em Inglês | MEDLINE | ID: mdl-29679602

RESUMO

Goat myosin light chain 6 (gMYL6) is a constituent of certain extracted immunization-induced goat anti-cancer bioactive peptides (ACBPs). However, little is known about its activity onto NK cells which are the basic cellular attackers in cancer immunotherapy for patients with malignancies. Because of the complicated extraction process and low yield of gMYL6 out of the goat ACBPs' mixture, the Nano-flow liquid chromatography and C-terminal polycationic tag expression strategy were used to identify and enrich the peptide to investigate its bioactivity against cancers/tumors. The solubility-enhanced gMYL6 fused with a hexa-lysine tag showed a capacity of promoting the NK cells' cytotoxicity, making it a novel promising heterogeneous peptide cytokine against cancers.


Assuntos
Antineoplásicos/química , Antineoplásicos/farmacologia , Citotoxicidade Imunológica/efeitos dos fármacos , Células Matadoras Naturais/imunologia , Lisina/química , Cadeias Leves de Miosina/química , Cadeias Leves de Miosina/farmacologia , Sequência de Aminoácidos , Animais , Antineoplásicos/isolamento & purificação , Clonagem Molecular , Expressão Gênica , Cabras , Humanos , Células K562 , Células Matadoras Naturais/efeitos dos fármacos , Cadeias Leves de Miosina/genética , Cadeias Leves de Miosina/isolamento & purificação , Proteínas Recombinantes/química , Proteínas Recombinantes/genética , Proteínas Recombinantes/isolamento & purificação , Proteínas Recombinantes/farmacologia , Solubilidade
20.
Drug Alcohol Depend ; 186: 201-206, 2018 May 01.
Artigo em Inglês | MEDLINE | ID: mdl-29605569

RESUMO

BACKGROUND: In response to calls to reassess and reform international drug policy, we estimated the prevalence of drug use among students in China from 2004 to 2013 through a systematic review and meta-analysis. METHODS: We systemically identified and reviewed published studies on illicit drug use and abuse of sedative-hypnotics among students in China. We estimated the prevalence of drug use among students using a meta-regression model. The trends in drug use and any geographic differences were assessed using multilevel models, and the association between drug use and sedative-hypnotics abuse was also examined. RESULTS: The pooled prevalence of illicit drug use and sedative-hypnotics abuse in students was 2.10% (95% CI: 1.80-2.50) and 6.10% (95% CI: 5.10-7.20). A significant decrease in the prevalence of illicit drug use was observed in 2009-2013 (OR = 0.28, 95% CI: 0.25-0.32). Students in higher grades showed a higher prevalence of both illicit drug use and sedative-hypnotics abuse than those in lower grades. Provincial differences in illicit drug use (OR = 2.66, 95% CI: 1.24-5.73) and sedative-hypnotics abuse (OR = 1.37, 95% CI: 1.05-1.79) were identified. The geographical area of illicit drug use was observed to be expanding. CONCLUSION: Prevalence of drug use among students is twice as high as in the general population of adults in China, indicating that drug use is becoming an urgent public health issue among the younger population. Further investigation among school students is needed.


Assuntos
Instituições Acadêmicas/tendências , Estudantes , Transtornos Relacionados ao Uso de Substâncias/diagnóstico , Transtornos Relacionados ao Uso de Substâncias/epidemiologia , Adolescente , China/epidemiologia , Feminino , Humanos , Hipnóticos e Sedativos/efeitos adversos , Masculino , Prevalência , Estudantes/psicologia , Transtornos Relacionados ao Uso de Substâncias/psicologia , Adulto Jovem
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