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Eur. j. psychol. appl. legal context (Internet) ; 12(1): 23-34, ene.-jun. 2020. tab
Artigo em Inglês | IBECS-Express | ID: ibc-ET1-4540


In a countermeasures experiment, we examined to what extent liars who learn about the Model Statement tool and about the proportion of complications (complications/complications + common knowledge details + self-handicapping strategies) can successfully adjust their responses so that they sound like truth tellers. Truth tellers discussed a trip they had made; liars fabricated a story. Participants were of Lebanese, Mexican, and South-Korean origin. Prior to the interview they did or did not receive information about (i) the working of the Model statement and (ii) three types of verbal detail: complications, common knowledge details and self-handicapping strategies. We found no evidence that liars sounded like truth tellers after being informed about the Model Statement and/or types of detail we examined. Actually, veracity differences were similar across experimental conditions, with truth tellers reporting more detail and more complications and obtaining a higher proportion of complications score than liars

En un experimento de contramedidas examinamos hasta qué punto los mentirosos que reciben información sobre la Declaración modelo y la proporción de complicaciones que presenta (complicaciones / complicaciones + detalles de conocimiento general + estrategias de autoobstaculización) pueden ajustar sus respuestas con éxito para que parezca que dicen la verdad. Los que dicen la verdad declararon sobre un viaje que habían hecho; los mentirosos inventaron una historia. Los participantes eran de origen libanés, mexicano y surcoreano. Antes de la entrevista habían recibido o no información sobre (i) el funcionamiento de la Declaración modelo y (ii) tres tipos de detalles verbales: complicaciones, detalles de conocimiento general y estrategias de autoobstaculización. No encontramos evidencia de que los mentirosos se parecieran a los que dicen la verdad después de ser informados sobre la Declaración modelo y los tipos de detalle que examinamos. En realidad, las diferencias de veracidad fueron semejantes en todas las condiciones experimentales: los que decían la verdad informaron con mayor detalle y de más complicaciones y obtuvieron una mayor puntuación en la proporción de complicaciones que los mentirosos

Acta Psychol (Amst) ; 177: 44-53, 2017 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-28477454


We examined how the presence of an interpreter during an interview affects eliciting information and cues to deceit, while using a method that encourages interviewees to provide more detail (model statement, MS). A total of 199 Hispanic, Korean and Russian participants were interviewed either in their own native language without an interpreter, or through an interpreter. Interviewees either lied or told the truth about a trip they made during the last twelve months. Half of the participants listened to a MS at the beginning of the interview. The dependent variables were 'detail', 'complications', 'common knowledge details', 'self-handicapping strategies' and 'ratio of complications'. In the MS-absent condition, the interviews resulted in less detail when an interpreter was present than when an interpreter was absent. In the MS-present condition, the interviews resulted in a similar amount of detail in the interpreter present and absent conditions. Truthful statements included more complications and fewer common knowledge details and self-handicapping strategies than deceptive statements, and the ratio of complications was higher for truth tellers than liars. The MS strengthened these results, whereas an interpreter had no effect on these results.

Decepção , Comportamento de Busca de Informação/fisiologia , Entrevistas como Assunto/métodos , Detecção de Mentiras , Adolescente , Adulto , Percepção Auditiva , Sinais (Psicologia) , Feminino , Humanos , Linguagem , Masculino , Adulto Jovem
Arterioscler Thromb Vasc Biol ; 36(9): 1928-36, 2016 09.
Artigo em Inglês | MEDLINE | ID: mdl-27470512


OBJECTIVE: Vascular smooth muscle cells (VSMCs) modulate their phenotype between synthetic and contractile states in response to environmental changes; this modulation plays a crucial role in the pathogenesis of restenosis and atherosclerosis. Here, we identified fibroblast growth factor 12 (FGF12) as a novel key regulator of the VSMC phenotype switch. APPROACH AND RESULTS: Using murine models and human specimens, we found that FGF12 was highly expressed in contractile VSMCs of normal vessel walls but was downregulated in synthetic VSMCs from injured and atherosclerotic vessels. In human VSMCs, FGF12 expression was inhibited at the transcriptional level by platelet-derived growth factor-BB. Gain- and loss-of-function experiments showed that FGF12 was both necessary and sufficient for inducing and maintaining the quiescent and contractile phenotypes of VSMCs. FGF12 inhibited cell proliferation through the p53 pathway and upregulated the key factors involved in VSMC lineage differentiation, such as myocardin and serum response factor. Such FGF12-induced phenotypic change was mediated by the p38 MAPK (mitogen-activated protein kinase) pathway. Moreover, FGF12 promoted the differentiation of mouse embryonic stem cells and the transdifferentiation of human dermal fibroblasts into SMC-like cells. Furthermore, adenoviral infection of FGF12 substantially decreased neointima hyperplasia in a rat carotid artery injury model. CONCLUSIONS: In general, FGF family members induce a synthetic VSMC phenotype. Interestingly, the present study showed the unanticipated finding that FGF12 belonging to FGF family, strongly induced the quiescent and contractile VSMC phenotypes and directly promoted VSMC lineage differentiation. These novel findings suggested that FGF12 could be a new therapeutic target for treating restenosis and atherosclerosis.

Doenças das Artérias Carótidas/metabolismo , Lesões das Artérias Carótidas/metabolismo , Diferenciação Celular , Plasticidade Celular , Fatores de Crescimento de Fibroblastos/metabolismo , Músculo Liso Vascular/metabolismo , Miócitos de Músculo Liso/metabolismo , Regiões 5' não Traduzidas , Animais , Apolipoproteínas E/deficiência , Apolipoproteínas E/genética , Becaplermina , Sítios de Ligação , Doenças das Artérias Carótidas/patologia , Lesões das Artérias Carótidas/genética , Lesões das Artérias Carótidas/patologia , Artéria Carótida Primitiva/metabolismo , Artéria Carótida Primitiva/patologia , Diferenciação Celular/efeitos dos fármacos , Linhagem da Célula , Plasticidade Celular/efeitos dos fármacos , Proliferação de Células , Células Cultivadas , Modelos Animais de Doenças , Células-Tronco Embrionárias/metabolismo , Fatores de Crescimento de Fibroblastos/genética , Genótipo , Humanos , Hiperplasia , Masculino , Camundongos Endogâmicos C57BL , Camundongos Knockout , Músculo Liso Vascular/efeitos dos fármacos , Músculo Liso Vascular/patologia , Miócitos de Músculo Liso/efeitos dos fármacos , Miócitos de Músculo Liso/patologia , Neointima , Fenótipo , Fosfatidilinositol 3-Quinase/metabolismo , Ligação Proteica , Proteínas Proto-Oncogênicas c-sis/farmacologia , Interferência de RNA , Ratos Sprague-Dawley , Transdução de Sinais , Transcrição Genética , Transfecção , Vasoconstrição , Proteínas Quinases p38 Ativadas por Mitógeno/metabolismo
Food Chem ; 134(4): 2497-503, 2012 Oct 15.
Artigo em Inglês | MEDLINE | ID: mdl-23442716


In this study, a new analytical method was developed based on gas chromatography-triple quadrupole tandem mass spectrometry (GC-MS/MS) and used to determine 32 multiclass pesticides in ginseng products. The analytical method was validated, yielding recovery rates in the range of 55.2-108.3%, with precision values expressed as relative standard deviation (RSD) lower or equal to 12% at the spiking levels of 30, 100, and 1000 µg/kg. Correlation coefficients and LOQs (limit of quantification) were in the range 0.9801-0.9989 and 0.15-70 g/kg, respectively. With these validation data and this method, multiresidue pesticides of ginseng samples (fresh ginseng (n=118), red ginseng (n=24), dried ginseng (n=10)) were analysed. Among them, the most frequently detected pesticide was tolclofos-methyl. Tolclofos-methyl was detected in 86.4% of fresh ginseng (18.25-404.5 µg/kg), 91.7% of red ginseng (13.14-119.4 µg/kg), and 87.5% of dried ginseng (23.15-3673 µg/kg).

Cromatografia Gasosa-Espectrometria de Massas/métodos , Panax/química , Resíduos de Praguicidas/química , Espectrometria de Massas em Tandem/métodos
Proteomics ; 9(7): 1827-40, 2009 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-19294698


In this study, various solvent systems were applied to obtain a high and consistent recovery rate of low molecular weight plasma proteins (LMPP) from human plasma. A buffer system containing 7 M urea, 2 M thiourea, 25 mM NH(4)HCO(3) + 20% ACN (pH 8.2) produced the highest recovery rate of LMPP. To validate the recovery of cut off membrane (COM) obtained using the urea buffer system, 27 different 30 kDa COMs were used to prepare the LMPP sample which were then subjected to 1-D SDS-PAGE. Statistical analysis showed that the buffer system with COM produced a consistent the recovery of LMPP. In addition, 2-DE analysis was also conducted to determine the relative intensity of each protein spot. When molecular weight ranges over 30 kDa and under 30 kDa were evaluated, 953 and 587 protein spots were observed in the gels, respectively, resulting in a total of 1540 protein spots being resolved. Identification of the major proteins were then performed using a nano-LC/MS system comprised of an HPLC system and an ESI-quadrupole IT MS equipped with a nano-ESI source.

Proteínas Sanguíneas/análise , Eletroforese em Gel Bidimensional , Ultrafiltração , Acetonitrilos/química , Adulto , Análise de Variância , Tampões (Química) , Cromatografia Líquida de Alta Pressão , Humanos , Masculino , Peso Molecular , Solventes/química , Espectrometria de Massas por Ionização por Electrospray , Ureia/química