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1.
J Colloid Interface Sci ; 608(Pt 2): 1813-1826, 2021 Oct 21.
Artigo em Inglês | MEDLINE | ID: mdl-34742090

RESUMO

Developing antimicrobial agents that can eradicate drug-resistant (DR) bacteria and provide sustained protection from DR bacteria is a major challenge. Herein, we report a mild pyrolysis approach to prepare carbon nanogels (CNGs) through polymerization and the partial carbonization of l-lysine hydrochloride at 270 °C as a potential broad-spectrum antimicrobial agent that can inhibit biopolymer-producing bacteria and clinical drug-resistant isolates and tackle drug resistance issues. We thoroughly studied the structures of the CNGs, their antibacterial mechanism, and biocompatibility. CNGs possess superior bacteriostatic effects against drug-resistant bacteria compared to some commonly explored antibacterial nanomaterials (silver, copper oxide, and zinc oxide nanoparticles, and graphene oxide) through multiple antimicrobial mechanisms, including reactive oxygen species generation, membrane potential dissipation, and membrane function disruption, due to the positive charge and flexible colloidal structures resulting strong interaction with bacterial membrane. The minimum inhibitory concentration (MIC) values of the CNGs (0.6 µg mL-1 against E. coli and S. aureus) remained almost the same against the bacteria after 20 passages; however, the MIC values increased significantly after treatment with silver nanoparticles, antibiotics, the bacteriostatic chlorhexidine, and especially gentamicin (approximately 140-fold). Additionally, the CNGs showed a negligible MIC value difference against the obtained resistant bacteria after acclimation to the abovementioned antimicrobial agents. The findings of this study unveil the development of antimicrobial CNGs as a sustainable solution to combat multidrug-resistant bacteria.

2.
Zhongguo Gu Shang ; 34(11): 1010-5, 2021 Jul 25.
Artigo em Chinês | MEDLINE | ID: mdl-34812016

RESUMO

OBJECTIVE: To observe the effeicacy and safety of percutaneous endoscopic translaminar lumbar interbody fusion (PE-TLIF) in the treatment of L5S1 intervertebral disc herniation. METHODS: The clinical data of 37 patients with L5S1 intervertebral disc herniation underwent PE-TLIF from January 2018 to December 2019 were retrospectively analyzed. There were 16 males and 21 females, aged from 30 to 68 years old, with a mean of (45.62±13.57) years;body mass index was from 19.5 to 28.8 kg/m2, with a mean of (24.33±3.51) kg/m2;the course of disease was from 18 to 48 months, with a mean of (27.18±6.65) months. Operation time, intraoperative blood loss, postoperative drainage and complication rate were recorded; visual analogue scale(VAS), Japanese Orthopaedic Association (JOA) score and Oswestry Disability Index(ODI) before operation, 1 week, 6 months, 1 year after operation were observed. RESULTS: The operation time ranged from 78 to 171 min, with a mean of (120.74±41.19) min;intraoperative blood loss ranged from 61 to 102 ml, with a mean of (85.26±25.44) ml;and postoperative drainage ranged from 35 to 98 ml, with a mean of (40.75±12.17) ml. Complications occurred in 8 patients, including 2 cases of nerve injury, 4 cases of pain aggravated, 1 case of internal fixation loosening, and 1 case of intervertebral space nonfusion. The VAS, JOA score and ODI significantly improved after 1 week, 6 months, and 1 year after operation (P<0.05). CONCLUSION: PE-TLIF for the treatment of L5S1 intervertebral disc herniation has the advantages of less intraoperative blood loss, less trauma, and satisfactory short-term curative effect. It can effectively improve the symptoms of patients. However, the incidence of complications in the actual clinical practice is frequent, and surgical indications need to be strictly grasped.


Assuntos
Degeneração do Disco Intervertebral , Deslocamento do Disco Intervertebral , Disco Intervertebral , Fusão Vertebral , Adulto , Idoso , Feminino , Humanos , Deslocamento do Disco Intervertebral/cirurgia , Vértebras Lombares/cirurgia , Masculino , Pessoa de Meia-Idade , Estudos Retrospectivos , Resultado do Tratamento
3.
Chemosphere ; : 132759, 2021 Nov 03.
Artigo em Inglês | MEDLINE | ID: mdl-34742753

RESUMO

The development of environmentally friendly adsorbents has been extensively carried out to overcome the detrimental effects of heavy metal accumulation, which has persistently become a global ecological problem. In pursuit of generating eco-friendly adsorbents, a green method for synthesizing thiamine functionalized-Fe3O4 (FT) was developed in this study. A one-step chemical oxidation and functionalization technique was used to prepare FT using the ammonia-containing solvent. A molar ratio of ammonia:Fe:thiamine of 15:1:1 was shown to produce FT15 with high yield, adsorptivity, and purity. XRD, XPS, FTIR, SEM, and SQUID characterization of FT15 revealed the formation of superparamagnetic thiamine functionalized Fe3O4 in their particles. This superparamagneticity facilitates the easy recovery of FT15 particles from the waste-containing solution by using an external magnetic force. The batch adsorption of Cu(II) onto FT15 showed the best fit with the Sips adsorption isotherm model with a maximum adsorption capacity of 426.076 mg g-1, which is 5.69-fold higher capacity than the control unmodified Fe3O4 (F15). After five adsorption-desorption cycles, the FT15 can maintain up to 1.95-fold higher capacity than the freshly synthesized F15. Observation on the physicochemical properties of the post-adsorption materials showed the contribution of an amine group, pyrimidine ring, and the thiazolium group of thiamine in boosting its adsorption capacity. This study provides important findings to advance the adsorptivity of magnetic adsorbents with promising recoverability from aqueous solution by employing naturally available and environmentally friendly compounds such as thiamine.

4.
Ann Clin Biochem ; : 45632211054745, 2021 Oct 31.
Artigo em Inglês | MEDLINE | ID: mdl-34719967

RESUMO

BACKGROUND: Therapeutic monitoring of lithium (Li) is important because of its narrow therapeutic range and therapeutic index. Here, the authors present the evaluation of an accurate method for the determination of lithium in serum. METHOD: Serum samples were diluted with 0.3% ultrapure nitric acid and were spiked with an internal standard germanium (Ge). The Li/Ge ratio was detected in He mode; we utilized standard addition method to quantify lithium in human serum. The new inductively coupled plasma mass spectrometry (ICP-MS) assay was characterized for linearity, specificity, imprecision, trueness, accuracy, and comparison. RESULTS: The correlation coefficients (r) of linearity were all > 0.9999. The specificity proved to be good. The total coefficients of variation (CV) were 1.11% and 0.49% for the two serum samples. The mean bias from target values of standard reference materials (SRM 956d) was -0.71% for Level I, -017% for Level II, and 2.20 for Level III. External Quality Assessment Scheme for Reference Laboratories in Laboratory Medicine (RELA) gave satisfied results for the new method. Comparison with the ion-selective electrode routine method got reasonable results. CONCLUSION: This high accuracy method is an attractive alternative for lithium measurement and can be used as a candidate reference method to improve quality of serum lithium in China.

5.
Appl Opt ; 60(29): 9110-9116, 2021 Oct 10.
Artigo em Inglês | MEDLINE | ID: mdl-34623993

RESUMO

A roll angle displacement measurement technique with high resolution for large-range measurement is presented. The proposed technique is based on the birefringence effect and phase detection of polarization interferometry with a polarization camera. The phase difference variation between the s- and p-polarized light induced by a rolling birefringent crystal can be obtained by a polarization camera. The roll angle displacement of the birefringent crystal can be determined from the detected phase difference variation. Several experiments were performed to evaluate the feasibility and performance of the proposed technique. The experimental results demonstrate that the resolution of the system can reach 0.003° with a 10° measurement range. The nonlinear and periodic errors of the system are also analyzed.

7.
Front Cardiovasc Med ; 8: 746225, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34692795

RESUMO

Background: Patients with sustained atrial high-rate episodes (AHRE) have a high risk of major adverse cardio/cerebrovascular events (MACCE). However, the prediction model and factors for the occurrence of AHRE are unknown. We aimed to identify independent factors and various risk models for predicting MACCE and AHRE. Methods: We retrospectively enrolled 314 consecutive patients who had cardiac implantable electronic devices (CIEDs). The primary endpoint was MACCE after AHRE ≥3, 6 min, and 6 h. Atrial high-rate episodes was defined as >175 bpm (Medtronic®) lasting ≥30 s. Multivariate Cox and logistic regression analysis with time-dependent covariates were used to determine variables associated with independent risk of MACCE and occurrence of AHRE ≥3 min, respectively. Results: One hundred twenty-five patients (39.8%) developed AHRE ≥3 min, 103 (32.8%) ≥6 min, and 55 (17.5%) ≥6 h. During follow-up (median 32 months), 77 MACCE occurred (incidence 9.20/100 patient years, 95% CI 5.66-18.39). The optimal AHRE cutoff value was 3 min for MACCE, with highest Youden index 1.350 (AUC, 0.716; 95% CI, 0.638-0.793; p < 0.001). Atrial high-rate episodes ≥3 min-6 h were independently associated with MACCE. HATCH score and left atrial diameter were independently associated with AHRE ≥3 min. The optimal cutoff for HATCH score was 3 and for left atrial diameter was 4 cm for AHRE ≥3 min. Conclusion: Patients with CIEDs who develop AHRE ≥3 min have an independently increased risk of MACCE. Comprehensive assessment using HATCH score and echocardiography of patients with CIEDs is warranted.

8.
Sci Rep ; 11(1): 18992, 2021 Sep 23.
Artigo em Inglês | MEDLINE | ID: mdl-34556728

RESUMO

Patients with atrial high-rate episodes (AHRE) have a high risk of neurologic events, although the causal role and optimal cutoff threshold of AHRE for major adverse cardio/cerebrovascular events (MACCE) are unknown. This study aimed to identify independent factors for AHRE and subsequent atrial fibrillation (AF) after documented AHRE. We enrolled 470 consecutive patients undergoing cardiac implantable electrical device (CIED) implantations. The primary endpoint was subsequent MACCE after AHRE ≥ 6 min, 6 h, and 24 h. AHRE was defined as > 175 beats per minute (bpm) (Medtronic®) or > 200 bpm (Biotronik®) lasting ≥ 30 s. Multivariate Cox regression analysis with time-dependent covariates was used to determine variables associated with independent risk of MACCE. The patients' median age was 76 year, and 126 patients (26.8%) developed AHRE ≥ 6 min, 63 (13.4%) ≥ 6 h, and 39 (8.3%) ≥ 24 h. During follow-up (median: 29 months), 142 MACCE occurred in 123 patients. Optimal AHRE cutoff value was 6 min, with highest Youden index for MACCE. AHRE ≥ 6 min ~ 24 h was independently associated with MACCE and predicted subsequent AF. Male gender, lower body mass index, or BMI, and left atrial diameter were independently associated with AHRE ≥ 6 min ~ 24 h. Patients with CIEDs who develop AHRE ≥ 6 min have an independently increased risk of MACCE. Comprehensive assessment of patients with CIEDs is warranted.

10.
Neurol Res ; : 1-10, 2021 Aug 25.
Artigo em Inglês | MEDLINE | ID: mdl-34431446

RESUMO

To systematically compare 27 ICH models with regard to mortality and functional outcome at 1-month, 3-month and 1-year after ICH. The validation cohort was derived from the Beijing Registration of Intracerebral Hemorrhage. Poor functional outcome was defined as modified Rankin Scale score (mRS) ≥3 at 1-month, 3-month and 1-year after ICH, respectively. The area under the receiver operating characteristic curve (AUROC) and Hosmer-Lemeshow goodness-of-fit test were used to assess model discrimination and calibration. A total number of 1575 patients were included. The mean age was 57.2 ± 14.3 and 67.2% were male. The median NIHSS score on admission was 11 (IQR: 3-21). For predicting mortality at 3-month after ICH, AUROC of 27 ICH models ranged from 0.604 to 0.856. In pairwise comparison, the ICH-FOS (0.856, 95%CI = 0.835-0.878, P < 0.001) showed statistically better discrimination than other models for mortality at 3-month after ICH (all P < 0.05). For predicting poor functional outcome (mRS≥3) at 3-month after ICH, AUROC of 27 ICH models ranged from 0.602 to 0.880. In pairwise comparison with other prediction models, the ICH-FOS was superior in predicting poor functional outcome at 3-month after ICH (all P < 0.001). The ICH-FOS showed the largest Cox and Snell R-square. Similar results were verified for mortality and poor functional outcome at 1-month and 1-year after ICH. Several risk models are externally validated to be effective for risk stratification and outcome prediction after ICH, especially the ICH-FOS, which would be useful tools for personalized care and clinical trial in ICH.

11.
Cell Biol Toxicol ; 2021 Aug 17.
Artigo em Inglês | MEDLINE | ID: mdl-34401974

RESUMO

Acetaminophen (APAP) overdose is a common cause of drug-induced liver injury (DILI). Ferroptosis has been recently implicated in APAP-induced liver injury (AILI). However, the functional role and underlying mechanisms of mitochondria in APAP-induced ferroptosis are unclear. In this study, the voltage-dependent anion channel (VDAC) oligomerization inhibitor VBIT-12 and ferroptosis inhibitors were injected via tail vein in APAP-injured mice. Targeted metabolomics and untargeted lipidomic analyses were utilized to explore underlying mechanisms of APAP-induced mitochondrial dysfunction and subsequent ferroptosis. As a result, APAP overdose led to characteristic changes generally observed in ferroptosis. The use of ferroptosis inhibitor ferrostatin-1 (or UAMC3203) and iron chelator deferoxamine further confirmed that ferroptosis was responsible for AILI. Mitochondrial dysfunction, which is associated with the tricarboxylic acid cycle and fatty acid ß-oxidation suppression, may drive APAP-induced ferroptosis in hepatocytes. APAP overdose induced VDAC1 oligomerization in hepatocytes, and protecting mitochondria via VBIT-12 alleviated APAP-induced ferroptosis. Ceramide and cardiolipin levels were increased via UAMC3203 or VBIT-12 in APAP-induced ferroptosis in hepatocytes. Knockdown of Smpd1 and Taz expression responsible for ceramide and cardiolipin synthesis, respectively, aggravated APAP-induced mitochondrial dysfunction and ferroptosis in hepatocytes, whereas Taz overexpression protected against these processes. By immunohistochemical staining, we found that levels of 4-hydroxynonenal (4-HNE) protein adducts were increased in the liver biopsy samples of patients with DILI compared to that in those of patients with autoimmune liver disease, chronic viral hepatitis B, and non-alcoholic fatty liver disease (NAFLD). In summary, protecting mitochondria via inhibiting VDAC1 oligomerization attenuated hepatocyte ferroptosis by restoring ceramide and cardiolipin content in AILI.

12.
ACS Omega ; 6(30): 20042-20052, 2021 Aug 03.
Artigo em Inglês | MEDLINE | ID: mdl-34368589

RESUMO

The present work aimed to evaluate the reactivity of natural bioflavonoid hesperidin (HSP) and synthetically derived XAV939 (XAV) against human hepatocellular carcinoma (HepG2), human breast cancer (MDA-MB231) cancer cell lines, and related molecular and pathological profiles. Data recorded revealed that the cytotoxic potential of the tested products was found to be cell type- and concentration-dependent. The half-maximal inhibitory concentration (IC50) value of the HSP-XAV mixture against MDA-MB231 was significantly decreased in the case of using the HSP-XAV mixture against the HepG2 cell line. Also, there was a significant upregulation of the phosphotumor suppressor protein gene (P53) and proapoptotic genes such as B-cell lymphoma-associated X-protein (Bax, CK, and Caspase-3), while antiapoptotic gene B-cell lymphoma (Bcl-2) was significantly downregulated compared with the untreated cell control. The cell cycle analysis demonstrated that DNA accumulation was detected mainly during the G2/M phase of the cell cycle accompanied with the elevated reactive oxygen species level in the treatment of HepG2 and MDA-MB231 cell lines by the HSP-XAV mixture, more significantly than that in the case of cell control. Finally, our finding suggests that both HSP and XAV939 and their mixture may offer an alternative in human liver and breast cancer therapy.

13.
BMC Cardiovasc Disord ; 21(1): 394, 2021 08 14.
Artigo em Inglês | MEDLINE | ID: mdl-34391394

RESUMO

BACKGROUND: Post cardiac injury syndrome (PCIS) is induced by myocardial infarction or cardiac surgery, as well as minor insults to the heart such as percutaneous coronary intervention (PCI), or insertion of a pacing lead. PCIS is characterized by pericarditis after injury to the heart. The relatively low incidence makes differential diagnosis of PCIS after PCI or implantation of a pacemaker a challenge. This report describes two typical cases of PCIS. CASE PRESENTATION: The first patient presented with signs of progressive cardiac tamponade that occurred two weeks after implantation of a permanent pacemaker. Echocardiography confirmed the presence of a moderate amount of newly-formed pericardial effusion. The second patient underwent PCI for the right coronary artery. However, despite an uneventful procedure, the patient experienced dyspnea, tightness of chest and cold sweats, and bradycardia two hours after the procedure. Echocardiography findings, which showed a moderate amount of newly-formed pericardial effusion, suggested acute cardiac tamponade, and compromised hemodynamics. Both patients recovered with medication. CONCLUSION: These cases illustrated that PCIS can occur after minor myocardial injury, and that the possibility of PCIS should be considered if there is a history of possible cardiac insult.

14.
Clin Chem Lab Med ; 59(10): 1728-1734, 2021 Sep 27.
Artigo em Inglês | MEDLINE | ID: mdl-34225391

RESUMO

OBJECTIVES: The US Centers for Medicare & Medicaid Services proposed in 2019 that glycated hemoglobin A1c (HbA1c) be a CLIA'88 regulated analyte. People who commented expressed concerns that the proposed acceptance limit (AL, HbA1c in NGSP unit) ±10% for proficiency testing (PT) would be unable to maintain already improved analytical performance and guarantee the clinical utility of HbA1c testing. Assessing impact of various ALs on PT performance is needed to provide scientific evidence for adopting an appropriate AL. METHODS: Ten patient EDTA-whole blood specimens were distributed to 318 and 336 laboratories in the 2018 and 2019 PT events organized by Shanghai Center for Clinical Laboratory (SCCL). HbA1c concentrations were measured by participants using various methodologies commonly used in the USA and China. Targets were determined using secondary reference measurement procedures (SRM) at SCCL. "Failed Results" were those outside the SRM-defined target ± AL (5% through 10%). Laboratories with Failed Results ≥2 out of five samples per PT event obtained Event Unsatisfactory Status. RESULTS: HbA1c target values ranged 33.3 mmol/mol (5.2 NGSP%) -102.2 mmol/mol (11.5 NGSP%) for 2018 event, and 33.3 mmol/mol (5.2 NGSP%) -84.7 mmol/mol (9.9 NGSP%) for 2019 event. Overall Laboratory Event Unsatisfactory Rates were 11.3-12.2%, 4.8-5.3%, 0.9-3.1%, 0.6-2.2%, 0.6-1.4% and 0.6-1.4%, at AL of ±5, ±6, ±7, ±8, ±9 and ±10%, respectively. CONCLUSIONS: The AL (in NGSP unit) of ±6% or ±7% for PT evaluation of HbA1c results would be appropriate, with satisfactory event scores for about 95% of participant laboratories in a PT event.

15.
Polymers (Basel) ; 13(13)2021 Jun 23.
Artigo em Inglês | MEDLINE | ID: mdl-34201884

RESUMO

The 'Back-to-nature' concept has currently been adopted intensively in various industries, especially the pharmaceutical industry. In the past few decades, the overuse of synthetic chemicals has caused severe damage to the environment and ecosystem. One class of natural materials developed to substitute artificial chemicals in the pharmaceutical industries is the natural polymers, including cellulose and its derivatives. The development of nanocelluloses as nanocarriers in drug delivery systems has reached an advanced stage. Cellulose nanofiber (CNF), nanocrystal cellulose (NCC), and bacterial nanocellulose (BC) are the most common nanocellulose used as nanocarriers in drug delivery systems. Modification and functionalization using various processes and chemicals have been carried out to increase the adsorption and drug delivery performance of nanocellulose. Nanocellulose may be attached to the drug by physical interaction or chemical functionalization for covalent drug binding. Current development of nanocarrier formulations such as surfactant nanocellulose, ultra-lightweight porous materials, hydrogel, polyelectrolytes, and inorganic hybridizations has advanced to enable the construction of stimuli-responsive and specific recognition characteristics. Thus, an opportunity has emerged to develop a new generation of nanocellulose-based carriers that can modulate the drug conveyance for diverse drug characteristics. This review provides insights into selecting appropriate nanocellulose-based hybrid materials and the available modification routes to achieve satisfactory carrier performance and briefly discusses the essential criteria to achieve high-quality nanocellulose.

16.
ACS Appl Mater Interfaces ; 13(30): 35494-35505, 2021 Aug 04.
Artigo em Inglês | MEDLINE | ID: mdl-34288640

RESUMO

Polymer nanocapsules, with a hollow structure, are increasingly finding widespread use as drug delivery carriers; however, quantitatively evaluating the bio-nano interactions of nanocapsules remains challenging. Herein, poly(ethylene glycol) (PEG)-based metal-phenolic network (MPN) nanocapsules of three sizes (50, 100, and 150 nm) are engineered via supramolecular template-assisted assembly and the effect of the nanocapsule size on bio-nano interactions is investigated using in vitro cell experiments, ex vivo whole blood assays, and in vivo rat models. To track the nanocapsules by mass cytometry, a preformed gold nanoparticle (14 nm) is encapsulated into each PEG-MPN nanocapsule. The results reveal that decreasing the size of the PEG-MPN nanocapsules from 150 to 50 nm leads to reduced association (up to 70%) with phagocytic blood cells in human blood and prolongs in vivo systemic exposure in rat models. The findings provide insights into MPN-based nanocapsules and represent a platform for studying bio-nano interactions.


Assuntos
Sangue/metabolismo , Estruturas Metalorgânicas/química , Nanocápsulas/química , Polietilenoglicóis/química , Pirogalol/análogos & derivados , Animais , Citometria de Fluxo/métodos , Ouro/química , Ouro/metabolismo , Ouro/farmacocinética , Ouro/toxicidade , Humanos , Masculino , Nanopartículas Metálicas/química , Nanopartículas Metálicas/toxicidade , Estruturas Metalorgânicas/metabolismo , Estruturas Metalorgânicas/farmacocinética , Estruturas Metalorgânicas/toxicidade , Camundongos , Nanocápsulas/toxicidade , Tamanho da Partícula , Polietilenoglicóis/metabolismo , Polietilenoglicóis/farmacocinética , Polietilenoglicóis/toxicidade , Pirogalol/metabolismo , Pirogalol/farmacocinética , Pirogalol/toxicidade , Células RAW 264.7 , Ratos Sprague-Dawley
17.
Sci Rep ; 11(1): 12021, 2021 06 08.
Artigo em Inglês | MEDLINE | ID: mdl-34103604

RESUMO

Nitrogen-grafting through the addition of glycine (Gly) was performed on a metal- phenolic network (MPN) of copper (Cu2+) and gallic acid (GA) to increase its adsorption capacity. Herein, we reported a one-step synthesis method of MPN, which was developed according to the metal-ligand complexation principle. The nitrogen grafted CuGA (Ng-CuGA) MPN was obtained by reacting Cu2+, GA, and Gly in an aqueous solution at a molar ratio of 1:1:1 and a pH of 8. Several physicochemical measurements, such as Fourier transform infrared (FTIR) spectroscopy, scanning electron microscopy (SEM), N2 sorption, X-ray diffraction (XRD), and thermal gravimetry analysis (TGA), were done on Ng-CuGA to elucidate its characteristics. The analysis revealed that the Ng-CuGA has non-uniform spherical shaped morphology with a pore volume of 0.56 cc/g, a pore size of 23.25 nm, and thermal stability up to 205 °C. The applicational potential of the Ng-CuGA was determined based on its adsorption capacity against methylene blue (MB). The Ng-CuGA was able to adsorb 190.81 mg MB per g adsorbent at a pH of 6 and temperature of 30 °C, which is 1.53 times higher than the non-grafted CuGA. Detailed assessment of Ng-CuGA adsorption properties revealed their pH- and temperature-dependent nature. The adsorption capacity and affinity were found to decrease at a higher temperature, demonstrating the exothermic adsorption behavior.

18.
Int J Mol Sci ; 22(11)2021 May 21.
Artigo em Inglês | MEDLINE | ID: mdl-34063782

RESUMO

In this study, we demonstrate the synthesis of carbonized nanogels (CNGs) from an amino acid (lysine hydrochloride) using a simple pyrolysis method, resulting in effective viral inhibition properties against infectious bronchitis virus (IBV). The viral inhibition of CNGs was studied using both in vitro (bovine ephemeral fever virus (BEFV) and pseudorabies virus (PRV)) and in ovo (IBV) models, which indicated that the CNGs were able to prevent virus attachment on the cell membrane and penetration into the cell. A very low concentration of 30 µg mL-1 was found to be effective (>98% inhibition) in IBV-infected chicken embryos. The hatching rate and pathology of IBV-infected chicken embryos were greatly improved in the presence of CNGs. CNGs with distinctive virus-neutralizing activities show great potential as a virostatic agent to prevent the spread of avian viruses and to alleviate the pathology of infected avian species.


Assuntos
Antivirais/farmacologia , Infecções por Coronavirus/tratamento farmacológico , Vírus da Bronquite Infecciosa/efeitos dos fármacos , Lisina/farmacologia , Nanogéis/administração & dosagem , Substâncias Protetoras/farmacologia , Animais , Linhagem Celular , Galinhas/virologia , Chlorocebus aethiops , Infecções por Coronavirus/virologia , Cricetinae , Vírus da Febre Efêmera Bovina/efeitos dos fármacos , Feminino , Herpesvirus Suídeo 1/efeitos dos fármacos , Doenças das Aves Domésticas/tratamento farmacológico , Doenças das Aves Domésticas/virologia , Ratos , Ratos Sprague-Dawley , Células Vero , Internalização do Vírus/efeitos dos fármacos , Replicação Viral/efeitos dos fármacos
19.
IEEE Trans Biomed Circuits Syst ; 15(3): 537-548, 2021 06.
Artigo em Inglês | MEDLINE | ID: mdl-34101596

RESUMO

This work presents a portable wireless urine detection system which consists of an electrochemical readout application specific integrated circuit (ASIC) and a biosensor composed of 2, 2'-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) and carbon nanotube (ABTS-CNT) for the detection of urine albumin-to-creatinine ratio (UACR). The ASIC includes a potentiostat, a digital circuitry and a power management circuit which can perform electrochemistry techniques with a dual-channel screen-printing carbon electrode (SPCE). Electrochemical experiments on the proposed biosensor (SPCE|ABTS-CNT|Nafion) have revealed promising sensing characteristics for creatinine and human serum albumin detection. Practical urine tests has demonstrated the capability of the proposed urine detection system for UACR detection with both the power-efficient readout ASIC and the ABTS-CNT biosensor. A user-friendly prototype has also been designed which can be useful for either personal health administrationor homecare.


Assuntos
Técnicas Biossensoriais , Nanotubos de Carbono , Benzotiazóis , Técnicas Eletroquímicas , Eletroquímica , Humanos , Ácidos Sulfônicos
20.
J Neurol ; 2021 May 21.
Artigo em Inglês | MEDLINE | ID: mdl-34019178

RESUMO

BACKGROUND: Persistent Postural-Perceptual Dizziness (PPPD) is one of the most common types of chronic dizziness. The pathogenesis remains unclear. OBJECTIVE: This study aimed to systematically review neuroimaging literature for investigating the central mechanism of PPPD and related disorders. METHODS: PubMed, EMBASE, Medline, Cochrane, and Web of Science were searched by the Preferred Reporting Items for Systematic Reviews and Meta-Analyses guidelines. The articles analyzing structural and functional neuroimaging features of PPPD and related disorders were selected according to eligibility criteria. RESULTS: Fifteen articles, including 4 structural, 10 functional, and 1 multimodal imaging, were eligible for inclusion in this review. The whiter matter alterations in PPPD are not entirely consistent. The changes of grey matter mainly in multisensory vestibular cortices, visual cortex, cerebellum, as well as anxiety-related network. Consistent with structural imaging, functional imaging conducted during the specific tasks or in the resting state has both found abnormal functional activation and connectivity in the vestibular cortex, especially in the parieto-insular vestibular cortex (PIVC), visual cortex, cerebellum, and anxiety-related network in PPPD and related disorder. CONCLUSIONS: The current review provides up-to-date knowledge and summarizes the possible central mechanism for PPPD and related disorders, and it is helpful to understanding the mechanism of PPPD.

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