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1.
Head Neck ; 44(2): 548-561, 2022 02.
Artigo em Inglês | MEDLINE | ID: mdl-34808012

RESUMO

To perform a systematic review focusing on the prognosis of oral cavity squamous cell carcinoma (OSCC) in young patients (≤40 years old) compared to older (>40 years old). Four databases were used in our search strategy. First, all titles were systematically organized using the Covidence platform online. In the second phase, 118 full texts of potentially eligible studies were analyzed by reviewers independently and in pairs. Twelve studies were considered eligible for data extraction. The relapse was higher in the young than in controls (pooled relative risk (RR) = 1.31; 95% CI [1.10-1.56]). The 5-year disease-free survival (DFS) was worse in young group (pooled hazard ratio (HR) = 0.73; 95% CI [0.63-0.85]) but the 5-year overall survival (OS) estimate was similar between the groups (pooled HR = 0.84; 95% CI [0.70-1.00]). While the 5-year OS was similar between groups, the number of relapses and 5-year DFS were worse in patients with OSCC ≤40 years old.


Assuntos
Carcinoma de Células Escamosas , Neoplasias de Cabeça e Pescoço , Neoplasias Bucais , Adolescente , Adulto , Carcinoma de Células Escamosas/terapia , Humanos , Neoplasias Bucais/terapia , Recidiva Local de Neoplasia , Prognóstico , Carcinoma de Células Escamosas de Cabeça e Pescoço , Adulto Jovem
3.
Head Neck Pathol ; 15(2): 663-667, 2021 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-32865727

RESUMO

A patient with an odontogenic fibroma (OF) with the presence of fibroblasts of varying morphology is presented, and the main microscopic differential diagnosis is discussed. Case report: a 51-year-old man complained of a swelling in the anterior region of the mandible. The panoramic radiograph showed a radiolucent, well-defined multilocular image, with displacement of the roots of the left mandibular incisors and canine. A cone beam computed tomography examination revealed a hypodense image, with delicate bone spicules inside, divergence of the tooth roots and destruction of the buccal cortical bone. Under the differential diagnosis of a desmoplastic ameloblastoma or a squamous odontogenic tumor, an incisional biopsy was made. Microscopically, deeply collagenized tissue with scarce odontogenic epithelium islands, large fibroblasts of varying morphology and calcified material was observed. A diagnosis of OF was made. Surgical removal was carried out, and the specimen was examined. Identical aspects were found, and the immunohistochemical reaction for pan-cytokeratin was carried out to identify the odontogenic epithelial cells. The definitive diagnosis of OF was confirmed, and we emphasize the importance of the correlation of all characteristics for a definitive diagnosis.


Assuntos
Fibroblastos/patologia , Fibroma/patologia , Tumores Odontogênicos/patologia , Humanos , Masculino , Mandíbula/patologia , Pessoa de Meia-Idade
4.
J Med Virol ; 93(6): 3835-3840, 2021 06.
Artigo em Inglês | MEDLINE | ID: mdl-32910471

RESUMO

Human papillomavirus (HPV) types 6 and 11 are the etiological agents of recurrent respiratory papillomatosis (RRP). We examined the prevalence and distribution of HPVs 6 and 11 genetic variants in juvenile onset (JORRP) and adult onset (AORRP) laryngeal papillomas. Cases of JORRP and AORRP were collected, retrospectively. HPV detection and genotyping were accessed by polymerase chain reaction-sequencing in 67 RRP samples. Overall, the most prevalent HPV-6 variants were from B1 (55.8%) and B3 (27.9%) sublineages, whereas among HPV-11 positive samples A2 (62.5%) variants were predominant. A higher prevalence of HPV-6 B1 was observed in JORRP (83.3% B1 and 16.7% B3), compared with AORRP cases (58.3% B1 and 41.7% B3). HPV-11 A2 variants were more prevalent both in JORRP (57.2%) and in AORRP cases (70.0%). Nevertheless, with the exception that HPV-6 B1 were significantly less likely to recur, there was a lack of association between any particular HPVs 6 or 11 variant and clinicopathological features. Our data do not support an association between HPVs 6 and 11 variability and RRP.


Assuntos
Variação Genética , Papillomavirus Humano 11/genética , Papillomavirus Humano 6/genética , Neoplasias Laríngeas/virologia , Papiloma/virologia , Infecções por Papillomavirus/epidemiologia , Infecções Respiratórias/epidemiologia , Infecções Respiratórias/virologia , Adolescente , Adulto , Feminino , Genótipo , Humanos , Masculino , Infecções por Papillomavirus/virologia , Prevalência , Estudos Retrospectivos , Adulto Jovem
5.
Braz. dent. sci ; 24(2): 1-7, 2021. tab
Artigo em Inglês | LILACS, BBO - Odontologia | ID: biblio-1178366

RESUMO

Objetive: The aim of this study was to assess the profile of dental students at the ICT/CSJC-UNESP School of Dentistry and their preferred studying style. Material and methods: Data on age, sex, place of residence, first-choice course, secondary education background and study method preference were collected from an online questionnaire given in the second year dental materials course (first class) of the day and evening courses of the years 2016 to 2019. All data were tabulated and submitted to one-way ANOVA and to t test, both with α= 0.05. Pearson correlation test was performed between demographic data and study method preference (α = 0.05). Results: Most of the students were women living in São José dos Campos city who had chosen Dentistry as their first-choice course. Many were originally from other cities and lived in shared accommodation with friends. The majority stated that private high school was their main educational background. The students' age and place of living showed to correlate with study method preference. Conclusion: Although ICT/CSJC-UNESP students were from millennial and post-millennial generations, students with public secondary education preferred studying alone, while those from private education preferred studying in group (AU).


Objetivo: O objetivo do estudo foi o de assessar o perfil de alunos do curso de Odontologia do ICT/CSJC-UNESP e seu método de estudo de preferência. Material e Métodos: Dados de idade, sexo, local de residência, primeira escolha de curso, formação escolar prévia e método de estudo de preferência foram coletados a partir de um questionário online aplicado no primeiro dia de aula aos alunos do segundo ano na disciplina de materiais odontológicos dos cursos integral e noturno entre 2016 e 2019. Os dados foram tabulados e submetidos a ANOVA um fator e ao teste t, ambos com α = 0,05. O teste de correlação de Pearson foi realizado para os dados demográficos e métodos de estudo de preferência (α= 0,05). Resultados: A maioria dos estudantes eram mulheres que moravam em São José dos Campos, quem tinham a Odontologia como a primeira escolha de curso. Muitos eram originariamente de outras cidades e compartilhavam moradia com amigos. A maioria relatou ter a escola particular como maior parte no ensino fundamental e médio. A idade do aluno e o local de residência mostraram estar correlacionadas ao método de estudo de preferência. Conclusão: Embora os estudantes do ICT/CSJC-UNESP sejam da geração "millennial" e "pós-millennial", aqueles com formação em escola secundária pública preferiram estudar sozinhos, enquanto que aqueles com formação em escola particular preferiram estudar em grupo (AU).


Assuntos
Humanos , Masculino , Feminino , Adulto , Estudantes de Odontologia , Universidades , Educação em Odontologia
6.
Pediatr Dermatol ; 37(4): 721-723, 2020 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-32372427

RESUMO

We report a case of atypical oral hairy leukoplakia (OHL) in a 9-year-old immunocompetent girl treated with fluticasone propionate nasal spray for allergic rhinitis. The OHL in childhood is uncommon and should be included in a differential diagnosis of white lesions in the oral mucosa.


Assuntos
Leucoplasia Pilosa , Sprays Nasais , Corticosteroides , Criança , Feminino , Fluticasona/efeitos adversos , Humanos , Leucoplasia Oral , Mucosa Bucal
7.
Laryngoscope ; 130(11): E611-E618, 2020 11.
Artigo em Inglês | MEDLINE | ID: mdl-31860132

RESUMO

OBJECTIVE: The objective was to investigate the prevalence of the Epstein-Barr virus (EBV) and its association with human papilloma virus (HPV) detection, clinicopathological features, and the severity of recurrent respiratory papillomatosis (RRP). METHODS: Cases of juvenile recurrent respiratory papillomatosis (JRRP) (n = 36) and adult recurrent respiratory papillomatosis (ARRP) (n = 44) were collected retrospectively and subdivided into low- and high-risk severity groups based on the Derkay score. We performed HPV detection and genotyping using a reverse hybridization protocol and investigated the presence of EBV by polymerase chain reaction (PCR) and in situ hybridization. CD21 levels were accessed by immunohistochemistry. RESULTS: All samples were HPV-positive, including 49 cases of HPV 6, 26 cases of HPV 11, four cases of HPV 6 and 11 coinfections, and one case of HPV 16. EBV-DNA was detected in nine samples by PCR, although none of the cases were positive by means of in situ hybridization. CD21 immunoexpression was not statistically associated with any of the variables analyzed. HPV 6 detection was significantly higher in ARRP cases (P = 0.03), whereas HPV 11 was more prevalent in JRRP cases (P = 0.02) and was even more prevalent in JRRP cases of greater severity (Derkay laryngoscopic scale ≥20) (P = 0.04). CONCLUSION: The presence of EBV does not seem to play an important role in the progression/severity of RRP. LEVEL OF EVIDENCE: 4 Laryngoscope, 130:E611-E618, 2020.


Assuntos
Alphapapillomavirus/genética , DNA Viral/análise , Herpesvirus Humano 4/genética , Infecções por Papillomavirus/virologia , Infecções Respiratórias/virologia , Índice de Gravidade de Doença , Adolescente , Adulto , Idoso , Criança , Pré-Escolar , Infecções por Vírus Epstein-Barr/epidemiologia , Infecções por Vírus Epstein-Barr/virologia , Feminino , Genótipo , Humanos , Hibridização In Situ , Lactente , Laringoscopia/estatística & dados numéricos , Masculino , Pessoa de Meia-Idade , Reação em Cadeia da Polimerase , Prevalência , Receptores de Complemento 3d/análise , Estudos Retrospectivos , Adulto Jovem
8.
Clin Oral Investig ; 24(2): 785-797, 2020 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-31154539

RESUMO

OBJECTIVES: Evaluate the modulating effect of ionizing radiation, blood cytokine levels, and bone remodeling of the interface around the implant to understand the radiation mechanisms which can impair the implants receptor site. MATERIAL AND METHODS: Sixty rats were submitted to grade V titanium implants in the femurs and were divided into the following groups: no-irradiation (N-Ir): control group with implant only; early-irradiation (E-Ir): implant + irradiation after 24 h; late-irradiation (L-Ir): implant + irradiation after 4 weeks; and previous-irradiation (P-Ir): irradiation + implant after 4 weeks. The animals in the E-Ir, L-Ir, and P-Ir groups were irradiated in two fractional stages of 15 Gy. At 3 days, 2 weeks, and 7 weeks after the final procedure, five animals were randomly euthanized per group. Serum levels of TNF-ɑ, IL-1ß, TGF-ß, IL-6, M-CSF, and IL-10 were measured from blood collected prior to euthanasia using the ELISA test. The pieces containing the implants were subjected to immunohistochemical labeling using the tartrate acid resistant to phosphatase, osteocalcin, and caspase-3 markers and mCT. The ANOVA test was used for statistical analysis, and the Tukey multiple comparison test (p < 0.05) was applied. RESULTS: The results indicated that ionizing radiation modifies the production of pro- and anti-inflammatory serum cytokines, the expression of proteins involved in bone remodeling and cellular apoptosis, as well as changes in bone formation. CONCLUSIONS: The results suggests that a longer period between radiotherapy and implant placement surgery when irradiation occurs prior to implant installation would allow the recovery and renewal of bone cells and avoid future failures in osseointegration. CLINICAL RELEVANCE: The search for modifications caused by ionizing irradiation in bone tissue can indicate the ideal period for implant placement without affecting the osseointegration process.


Assuntos
Implantes Dentários , Animais , Fêmur , Implantes Experimentais , Osseointegração , Osteogênese , Ratos , Titânio
9.
Am J Otolaryngol ; 41(2): 102321, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-31787356

RESUMO

OBJECTIVE: To characterize inflammatory cells in Recurrent Respiratory Papillomatosis (RRP) and to correlate it with severity using the Derkay laryngoscopic scale. MATERIALS AND METHODS: The data and biopsies from 36 patients with Juvenile (JRRP) and 56 patients with Adult (ARRP) were collected and analyzed under light microscopy. The patients were separated into groups according to the Derkay index: ≥20 for the most severe and < 20 for the less severe cases. Immunohistochemical analysis using CD3, CD4, CD8, CD15, CD20, CD68, FoxP3 and MUM-1 antibodies was performed, and the inflammatory cells were quantified. All the clinicopathological characteristics and the results of the immunohistochemical analysis were compared among the groups proposed using the Chi-Square test and correlated through the Spearman correlation test. RESULTS: The ARRP showed significantly higher quantities of CD3+, CD8+ and MUM1+ cells (p < .05) than the JRRP samples. The presence of CD15+ cells showed positive correlation with the Derkay index (p < .05), while the MUM-1+ cells showed an inverse correlation (p = .01). CONCLUSION: There are differences between the inflammatory cells population in the juvenile and adult groups and it can be related to disease severity.


Assuntos
Papiloma/patologia , Neoplasias do Sistema Respiratório/patologia , Adulto , Autoanticorpos , Complexo CD3 , Antígenos CD4 , Antígenos CD8 , Criança , Pré-Escolar , Feminino , Humanos , Lactente , Inflamação , Fatores Reguladores de Interferon/imunologia , Laringoscopia , Antígenos CD15 , Masculino , Pessoa de Meia-Idade , Recidiva Local de Neoplasia , Papiloma/metabolismo , Papiloma/virologia , Papillomaviridae , Neoplasias do Sistema Respiratório/metabolismo , Neoplasias do Sistema Respiratório/virologia , Índice de Gravidade de Doença
10.
J Clin Exp Dent ; 11(6): e582-e586, 2019 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-31346382

RESUMO

Vascular leiomyomas (VL) are benign lesions of perivascular origin. We report two new cases and discuss their clinical, histological and immunohistochemical characteristics, in order to facilitate the diagnosis and treatment of such lesions. The patients, both male, presented asymptomatic nodules located in the bottom of the labial sulcus and buccal mucosa. In the second case, color doppler ultrasonography was performed, which showed no change in blood flow. After excisional biopsies, a limited lesion was observed histologically, with multiple tortuous vessels of varying sizes and calibers, and among them, spindle cells bundles, positive for smooth muscle actin. Oral VLs have clinical features similar to those of other more common lesions, making preoperative diagnosis difficult. Imaging examination, such as color doppler ultrasonography, may help in diagnosis. In general, excisional biopsy is performed, due to the ease of removal of the lesion during surgery. The treatment of choice is the complete excision of the lesion, which has an excellent prognosis and a low rate of recurrence. Key words:Vascular leiomyoma, spindle cells, thrombus, Doppler.

11.
Head Neck ; 41(5): 1412-1417, 2019 05.
Artigo em Inglês | MEDLINE | ID: mdl-30623508

RESUMO

BACKGROUND: Laryngeal papillomatosis (LP) is a disease that presents in both juvenile (JLP) and adult patients (ALP). This study correlated papillomatosis characteristics with the Derkay score. METHODS: Retrospective data and biopsies of 36 patients with JLP and 56 with ALP were collected and separated into groups according to their scores. RESULTS: The mean of the Derkay score, in the JLP group was 10.97 and in Group ALP was 8.26. The JLP group presented a more aggressive result than in the adult group (P = .02). In the JLP group, the respiratory difficulty (P = .01) and tracheostomy were correlated to a higher Derkay score (P < .05). Microscopically, the JLP samples presented a higher incidence of atypical mitosis and mitosis above the basal cells layer of the epithelium (P < .05) and these characteristics were correlated with a higher Derkay index (P = .03). CONCLUSION: Findings suggest that ALP and JLP can present different clinical courses and histopathological features. There was a higher degree of LP severity in JLP.


Assuntos
Transformação Celular Neoplásica/patologia , Progressão da Doença , Neoplasias Laríngeas/patologia , Papiloma/patologia , Adolescente , Fatores Etários , Biópsia por Agulha , Brasil/epidemiologia , Criança , Pré-Escolar , Bases de Dados Factuais , Feminino , Guatemala/epidemiologia , Humanos , Imuno-Histoquímica , Internacionalidade , Neoplasias Laríngeas/epidemiologia , Masculino , Papiloma/epidemiologia , Prevalência , Prognóstico , Doenças Raras , Estudos Retrospectivos , Medição de Risco , Fatores Sexuais , Estatísticas não Paramétricas
12.
Appl Immunohistochem Mol Morphol ; 27(7): 537-542, 2019 08.
Artigo em Inglês | MEDLINE | ID: mdl-29189260

RESUMO

Oral potentially malignant disorders (OPMD) may develop malignant characteristics and transform into oral squamous cell carcinoma (OSCC) in a range of 1% to 2% of cases. Chronic alcohol consumption is associated with carcinogenesis, but its mechanism has not yet been fully elucidated. ALDH1A1 and 2, isoenzymes responsible for aldehyde oxidation involved in ethanol metabolism may be associated with the development of malignant head and neck neoplasms. The aim of this study was to analyze the expression of ALDH1A1 and ALDH2 in oral leukoplakia with epithelial dysplasia (OLP) and OSCC. A retrospective study was conducted on 27 cases of OLP and 30 cases of OSCC. Clinical data were obtained from medical records, and all cases were classified as mild, moderate, and severe for OLP, and well-differentiated, moderately differentiated, or poorly differentiated for OSCC cases. The ALDH1A1 and ALDH2 expression in OLP and OSCC was evaluated by the immunohistochemical technique. There was predominance of the male sex, in both OLP and OSCC cases. Oral tongue was the most affected site in both groups. OLP showed positive protein expression of ALDH1A1 in all cases, both basal and suprabasal epithelial layers, whereas ALDH2 showed less protein expression. In OSCC, the immunohistochemical reaction for ALDH1A1 expression was negative in 70%, whereas ALDH2 expression was positive in all cases. This study demonstrated the gradual loss of ALDH1A1 expression in OSCC in comparison with OLP, and the increased ALDH2 expression in OSCC.


Assuntos
Família Aldeído Desidrogenase 1/biossíntese , Aldeído-Desidrogenase Mitocondrial/biossíntese , Carcinoma de Células Escamosas , Regulação Enzimológica da Expressão Gênica , Regulação Neoplásica da Expressão Gênica , Leucoplasia Oral , Proteínas de Neoplasias/biossíntese , Retinal Desidrogenase/biossíntese , Neoplasias da Língua , Adulto , Idoso , Carcinoma de Células Escamosas/enzimologia , Carcinoma de Células Escamosas/patologia , Epitélio/enzimologia , Epitélio/patologia , Feminino , Humanos , Leucoplasia Oral/enzimologia , Leucoplasia Oral/patologia , Masculino , Pessoa de Meia-Idade , Estudos Retrospectivos , Neoplasias da Língua/enzimologia , Neoplasias da Língua/patologia
13.
Artigo em Inglês | MEDLINE | ID: mdl-29602688

RESUMO

OBJECTIVE: The aim of this study was to investigate alterations in the EGFR gene and its protein expression for a better understanding of the biologic behavior of ameloblastoma. STUDY DESIGN: Twenty-five samples of ameloblastoma were selected, and dual-color fluorescence in situ hybridization assay was performed. The results of the assay and immunohistochemistry reaction for EGFR and Ki67 were associated with clinicopathologic features and recurrence. RESULTS: All analyzed cases presented disomy without any gene polysomy or amplification. With regard to EGFR immunoexpression, 3 cases (12%) were considered negative, and 22 (88%) were positive, of which 13 (52%) were weak and 9 (36%) were strong. All samples presented low positivity for Ki67. There was no association between EGFR expression and clinicopathologic features or recurrence (P > .05). In some cases, EGFR immunoexpression was observed without gene amplification. CONCLUSIONS: Ameloblastoma development, progression, or recurrence does not appear to be related to EGFR amplification or polysomy.


Assuntos
Ameloblastoma/metabolismo , Receptores ErbB/metabolismo , Neoplasias Mandibulares/metabolismo , Adolescente , Adulto , Idoso , Biomarcadores Tumorais/metabolismo , Criança , Feminino , Amplificação de Genes , Humanos , Imuno-Histoquímica , Hibridização in Situ Fluorescente , Antígeno Ki-67/metabolismo , Masculino , Pessoa de Meia-Idade , Estudos Retrospectivos
14.
Artigo em Inglês | MEDLINE | ID: mdl-29501355

RESUMO

OBJECTIVE: Tobacco and alcohol consumption are considered the main risk factors for oral squamous cell carcinoma (OSCC); however, the role of these factors in patients younger than 40 years is controversial, so it has been suggested that genomic instability and high-risk human papillomavirus (HR-HPV) infection may be contributing factors to oral carcinogenesis at a young age. Therefore, the aim of this study was to evaluate the immunoexpression of cell cycle proteins according HPV status in OSCC affecting young patients. METHODS: A tissue microarray construction based on 34 OSCC samples from young patients (<40 years old) was subjected to immunohistochemical reactions for Ki67, cyclin D1, C-ErbB2, p21, Myc, epidermal growth factor receptor, p53, and p16 antibodies. RESULTS: The clinicopathologic features and the immunoexpression of all tested proteins were similar in both groups. Patients with HPV-related OSSC tended to have better cancer-specific survival (CSS; 39% vs 60% 5-y CSS), and overall survival (OS; 29.2% vs 60% 5-year OS). However, this difference was not statistically significant. CONCLUSION: No significant difference exists in the expression of cell cycle proteins studied between HR-HPV DNA-positive and HR-HPV DNA-negative OSCC affecting young patients.


Assuntos
Carcinoma de Células Escamosas/virologia , Proteínas de Ciclo Celular/metabolismo , Neoplasias Bucais/virologia , Adulto , Biomarcadores Tumorais/metabolismo , Feminino , Humanos , Imuno-Histoquímica , Masculino , Projetos Piloto , Estudos Retrospectivos , Análise Serial de Tecidos
15.
J Prosthodont ; 27(4): 335-339, 2018 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-27434551

RESUMO

PURPOSE: To evaluate the effect of replacement of inadequate complete dentures on salivary flow and taste perception in geriatric patients. MATERIALS AND METHODS: Thirty-three patients, 13 males and 20 females, with a mean age of 64.4 years were submitted to stimulated and unstimulated salivary flow rate and salivary pH measurements, and sense of taste evaluation. Tests were performed 3 months before complete denture substitution and 3 weeks after denture insertion. RESULTS: The mean for unstimulated saliva (USS) was 2.1 ml before and 2.7 ml after replacement (p = 0.003). The mean volume of stimulated saliva was 6.3 ml before and 8.2 ml after replacement (p = 0.004). The pH mean of USS was 7.8 ± 0.44 before and 8.02 ± 0.41 after replacement (p = 0.005). No statistically significant difference was determined in the sense of taste before and 3 weeks after complete denture replacement. CONCLUSIONS: The replacement of inadequate complete dentures increases saliva flow; however, it does not improve taste perception.


Assuntos
Prótese Total , Salivação , Paladar , Adulto , Idoso , Feminino , Humanos , Masculino , Pessoa de Meia-Idade
16.
J Oral Pathol Med ; 47(1): 32-39, 2018 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-28626969

RESUMO

OBJECTIVES: Oral squamous cell carcinoma (OSCC) predominantly affects males in the fifth decade of life; nevertheless, an increased incidence in young patients has been reported worldwide, and the clinical and behavioral characteristics of tumors in this group are controversial, and the literature shows divergent results. PURPOSE: To investigate the clinicopathological features and prognostic significance of the immunoexpression of cell cycle and local invasion proteins in OSCC affecting young patients (≤40 years old). METHODS: A tissue microarray was performed with 132 OSCC samples (61 cases of young patients vs 71 cases of elderly patients) and submitted to immunohistochemical reactions with Ki67, p53, p16, Bcl-2, Cyclin D1, C-ErbB2, p21, Myc, EGFR, MMP-9, SMA, Cathepsin K and FGF-2 antibodies. RESULTS: Clinicopathological features and survival rates were similar in both groups. Although overexpression of EGFR (P=.042) and MMP-9 (P=.001) was more frequent in young patients, only C-ErbB-2 (P=.048) and SMA (P=.048) expression correlated with lower disease-free survival (DFS) in this group of patients. CONCLUSION: Clinicopathological features and survival rates are similar between younger and older patients with OSCC. The different patterns of C-ErbB2, EGFR, MMP-9, and SMA expression between the groups merits further investigation to understand their role in the early tumor onset in young patients.


Assuntos
Carcinoma de Células Escamosas/metabolismo , Pontos de Checagem do Ciclo Celular/fisiologia , Regulação Neoplásica da Expressão Gênica/fisiologia , Neoplasias Bucais/metabolismo , Adulto , Idoso , Idoso de 80 Anos ou mais , Brasil , Carcinoma de Células Escamosas/genética , Carcinoma de Células Escamosas/patologia , Catepsina K/metabolismo , Pontos de Checagem do Ciclo Celular/genética , Ciclina D1/metabolismo , Inibidor p16 de Quinase Dependente de Ciclina/metabolismo , Inibidor de Quinase Dependente de Ciclina p21/metabolismo , Intervalo Livre de Doença , Células Epiteliais/patologia , Receptores ErbB/metabolismo , Feminino , Fator 2 de Crescimento de Fibroblastos/metabolismo , Regulação Neoplásica da Expressão Gênica/genética , Humanos , Imuno-Histoquímica , Antígeno Ki-67/metabolismo , Masculino , Metaloproteinase 9 da Matriz/metabolismo , Pessoa de Meia-Idade , Neoplasias Bucais/genética , Neoplasias Bucais/mortalidade , Neoplasias Bucais/patologia , Proteínas de Neoplasias/genética , Proteínas de Neoplasias/metabolismo , Prognóstico , Proteínas Proto-Oncogênicas c-bcl-2/metabolismo , Proteínas Proto-Oncogênicas c-myc/metabolismo , Receptor ErbB-2/metabolismo , Taxa de Sobrevida , Proteína Supressora de Tumor p53/metabolismo
17.
Case Rep Otolaryngol ; 2018: 4362162, 2018.
Artigo em Inglês | MEDLINE | ID: mdl-30662782

RESUMO

Background: The malignant transformation of laryngeal papillomatosis (LP) into squamous cell carcinoma (SCC) can occur in up to 4% of LP cases. The low-risk HPV types 6 and 11 are those that are most commonly related to LP; however, high-risk HPV types may be present. The present study reviews the literature on cases of malignant transformation of LP in adults and reports a clinical case. Case Report: A 47-year-old male patient exhibiting hoarseness for 4 months presented an exophytic lesion in the right palatine tonsil and a digitiform-like lesion in the right vocal fold. The biopsy revealed a well-differentiated SCC in the vocal cord, which showed a transition zone with a squamous papillomatous lesion. By using the chromogenic in situ hybridization (CISH) test, both lesions showed a positive result for high-risk HPV types 16 and 18 and negative for low-risk HPV types 6 and 11. The final diagnosis was SCC arising from LP. The patient underwent surgical treatment. After 36 months of follow-up, no signs of recurrence were observed. Results: The literature review revealed 25 cases of malignant transformation into SCC of LP with adult onset. Of these, only 9 cases were assessed by CISH and/or PCR for HPV identification, of which 7 were positive. The current study focuses on the eighth case, suggesting the involvement of the high-risk HPV types in its pathogenesis. Conclusions: LP is considered a benign lesion with the potential for malignant transformation, which reinforces the need for its early diagnosis and the constant monitoring of patients with LP.

18.
Braz Oral Res ; 31: e53, 2017 Jul 03.
Artigo em Inglês | MEDLINE | ID: mdl-28678972

RESUMO

Peripheral ossifying fibroma (POF) is a reactive lesion of oral tissues, associated with local factors such as trauma or presence of dental biofilm. POF treatment consists of curettage of the lesion combined with root scaling of adjacent teeth and/or removal of other sources of irritants. This study aimed to analyze the clinical and pathological features of POF and to investigate the immunoexpression of Osterix and STRO-1 proteins. Data such as age, gender, and size were obtained from 30 cases of POF. Microscopic features were assessed by conventional light microscopy using hematoxylin-eosin staining and immunohistochemical markers, and by polarized light microscopy using Picrosirius red staining. The age range was 11-70 years and 70% of the patients were female. Moreover, the size of POF varied from 0.2 to 5.0 cm; in 43.33% of the cases, the mineralized content consisted exclusively of bony trabeculae. The immunohistochemical analysis showed nuclear staining for Osterix in 63% and for STRO-1 in 20% of the cases. Mature collagen fibers were observed in mineralized tissue in 76.67% of the cases. The clinical and microscopic features observed were in agreement with those described in the literature. Osterix was overexpressed, while STRO-1 was poorly expressed. Osterix was expressed particularly in cells entrapped in and around mineralized tissue, indicating the presence of a stimulus that triggers the differentiation of these cells into osteoblasts or cementoblasts, i.e., cells that produce mineralized tissue. Based on our results, Osterix may play a role in the pathogenesis of POF.


Assuntos
Antígenos de Superfície/fisiologia , Neoplasias Ósseas/patologia , Fibroma Ossificante/patologia , Fatores de Transcrição/fisiologia , Adolescente , Adulto , Idoso , Antígenos de Superfície/análise , Diferenciação Celular , Criança , Colágeno/análise , Feminino , Gengiva/patologia , Humanos , Imuno-Histoquímica , Masculino , Microscopia de Polarização , Pessoa de Meia-Idade , Osteoblastos/patologia , Fator de Transcrição Sp7 , Fatores de Transcrição/análise , Adulto Jovem
19.
Braz. oral res. (Online) ; 31: e53, 2017. tab, graf
Artigo em Inglês | LILACS | ID: biblio-952104

RESUMO

Abstract Peripheral ossifying fibroma (POF) is a reactive lesion of oral tissues, associated with local factors such as trauma or presence of dental biofilm. POF treatment consists of curettage of the lesion combined with root scaling of adjacent teeth and/or removal of other sources of irritants. This study aimed to analyze the clinical and pathological features of POF and to investigate the immunoexpression of Osterix and STRO-1 proteins. Data such as age, gender, and size were obtained from 30 cases of POF. Microscopic features were assessed by conventional light microscopy using hematoxylin-eosin staining and immunohistochemical markers, and by polarized light microscopy using Picrosirius red staining. The age range was 11-70 years and 70% of the patients were female. Moreover, the size of POF varied from 0.2 to 5.0 cm; in 43.33% of the cases, the mineralized content consisted exclusively of bony trabeculae. The immunohistochemical analysis showed nuclear staining for Osterix in 63% and for STRO-1 in 20% of the cases. Mature collagen fibers were observed in mineralized tissue in 76.67% of the cases. The clinical and microscopic features observed were in agreement with those described in the literature. Osterix was overexpressed, while STRO-1 was poorly expressed. Osterix was expressed particularly in cells entrapped in and around mineralized tissue, indicating the presence of a stimulus that triggers the differentiation of these cells into osteoblasts or cementoblasts, i.e., cells that produce mineralized tissue. Based on our results, Osterix may play a role in the pathogenesis of POF.


Assuntos
Humanos , Masculino , Feminino , Criança , Adolescente , Adulto , Idoso , Adulto Jovem , Fatores de Transcrição/fisiologia , Neoplasias Ósseas/patologia , Fibroma Ossificante/patologia , Antígenos de Superfície/fisiologia , Osteoblastos/patologia , Fatores de Transcrição/análise , Imuno-Histoquímica , Diferenciação Celular , Colágeno/análise , Fator de Transcrição Sp7 , Gengiva/patologia , Microscopia de Polarização , Pessoa de Meia-Idade , Antígenos de Superfície/análise
20.
Braz. dent. sci ; 20(4): 25-31, 2017. ilus
Artigo em Inglês | LILACS, BBO - Odontologia | ID: biblio-877836

RESUMO

Objective: Oral squamous cell carcinoma (OSCC) is one of the most common malignant neoplasms, with 300,000 new cases annually diagnosed in the world. Although it appears in the fifth position among men in Brazil, late diagnosis continues to be a leading cause of poor prognosis. We reviewed the literature to evaluate factors that contribute to diagnostic delay in oral cancer considering aspects related to the patient, health team and public health system. Material and Methods: The Medline (PubMed), Scopus, Web of Science and Embase databases were searched in the last 5 years using the following MeSH descriptors: carcinoma, squamous cell; mouth neoplasms; delayed diagnosis. The articles should contain in their objectives factors contributing to the delayed diagnosis of OSCC related to the patient, healthcare professionals and public health system in general. Results: One hundred twenty articles were retrieved and 27 on the delayed diagnosis of oral cancer were eligible. Conclusion: The literature showed that the delay in the diagnosis and treatment of patients with oral cancer could be minimized by providing health information, particularly about risk factors, by improving the training and retraining of physicians and dentists and by improving access to the health system. The communication technologies such as telemedicine, can play a key role in the early diagnosis of oral cancer. (AU)


Objetivo: o carcinoma de células escamosas de cavidade bucal (CCECB) é uma das neoplasias malignas mais comuns, com cerca de 300 mil novos casos diagnosticados anualmente no mundo. Embora esteja na quinta posição entre os mais frequentes em homens no Brasil, o diagnóstico tardio continua a ser a principal causa de mau prognóstico. Revisamos a literatura para avaliar fatores que possam contribuir para o atraso de diagnóstico no câncer bucal considerando aspectos relacionados ao paciente, equipe de saúde e o Sistema de Saúde Pública. Material e Métodos: Foram pesquisados os bancos de dados Medline (PubMed), Scopus, Web of Science e Embase nos últimos 5 anos utilizando os seguintes descritores MeSH: carcinoma de células escamosas, neoplasias de boca e atraso diagnóstico. Os artigos deveriam conter em seus objetivos fatores que possam ter contribuído para o diagnóstico tardio do CCECB relacionado ao paciente, aos profissionais de saúde e ao Sistema de Saúde Pública em geral. Resultados: Cento e vinte artigos foram recuperados, sendo elegíveis, vinte e sete relacionados ao diagnóstico tardio de câncer bucal. Conclusão: a literatura mostrou que o atraso no diagnóstico e tratamento de pacientes com câncer bucal poderia ser minimizado, fornecendo informações sobre saúde, particularmente sobre fatores de risco a população, melhorando o treinamento e reciclagem de médicos e dentistas e melhorando o acesso ao sistema de saúde. As tecnologias de comunicação, dentre elas a telemedicina, pode desempenhar um papel fundamental no diagnóstico precoce de câncer bucal. (AU)


Assuntos
Carcinoma de Células Escamosas , Diagnóstico Tardio , Neoplasias Bucais
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