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1.
Zhongguo Zhong Yao Za Zhi ; 44(19): 4142-4151, 2019 Oct.
Artigo em Chinês | MEDLINE | ID: mdl-31872691

RESUMO

Maca( Lepidium meyenii) known as the " national treasure of Peru" and " South American ginseng",is annual or biennial herbs of the genus Lepidium in Cruciferae. It mainly contains proteins,amino acids,polysaccharides,alkaloids( including:macamides,imidazoles,hydroxypyridines,carbazoles,organic amines and so on),glucosinolates,macaenes,thioethylurea,sterols and other chemical constituents. In recent years,more and more studies have found that it could treat osteoporosis and improve prostatehyperplasia,and possessed anti-cancer,female climacteric syndrome,rheumatism,antioxidant and other pharmacological effects. In this paper,the chemical constituents and bioactivity of Maca were reviewed,which could provide the basis for the further development and utilization of Maca.


Assuntos
Asteraceae , Lepidium , Extratos Vegetais , Antioxidantes , Peru
2.
R Soc Open Sci ; 6(6): 190150, 2019 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-31312486

RESUMO

Andrographis paniculata is an important traditional medicinal herb in South and Southeast Asian countries with diverse pharmacological activities that contains various flavonoids and flavonoid glycosides. Glycosylation can transform aglycones into more stable, biologically active and structurally diverse glycosides. Here, we report three glycosyltransferases from the leaves of A. paniculata (ApUFGTs) that presented wide substrate spectra for flavonoid glycosylation and exhibited multi-site glycosylation on the substrate molecules. They acted on the 7-OH position of the A ring and were able to glycosylate several other different types of compounds. The biochemical properties and phylogenetic analysis of these glycosyltransferases were also investigated. This study provides a basis for further research on the cloning of genes involved in glycosylation from A. paniculata and offers opportunities for enhancing flavonoid glycoside production in heterologous hosts. These enzymes are expected to become effective tools for drug discovery and for the biosynthesis of derivatives via flavonoid glycosylation.

3.
J Pharm Biomed Anal ; 170: 83-88, 2019 Jun 05.
Artigo em Inglês | MEDLINE | ID: mdl-30909057

RESUMO

Flos Lonicerae Japonicae (FLJ), known as golden-and-silver honeysuckle, is a widely used Chinese herbal medicine with pharmacological activities and edibleness in China. Chlorogenic acid (CGA) and luteoloside are the quality control markers of FLJ regulated by the Chinese Pharmacopoeia (2015 edition). For rapid evaluation of the quality of FLJ, dipsticks for CGA and luteoloside detection were developed. The detection limit of the dipsticks for CGA and luteoloside, defined as the lowest concentration of the target analyte between which the test line was invisible, were 100 ng/mL and 200 ng/mL, respectively. The dipsticks were used for determination of CGA and luteoloside contents in FLJ, and the results were confirmed by high-performance liquid chromatography. The developed dipsticks, with their simplicity of use, lack of dependence on instruments and environmental friendliness, could be used to evaluate the quality of FLJ within 10 min.


Assuntos
Ácido Clorogênico/química , Glucosídeos/química , Coloide de Ouro/química , Imunoensaio/métodos , Lonicera/química , Luteolina/química , Extratos Vegetais/química , China , Cromatografia Líquida de Alta Pressão/métodos , Medicamentos de Ervas Chinesas/química , Flores/química , Controle de Qualidade
4.
Nat Prod Res ; 33(23): 3409-3416, 2019 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-29863900

RESUMO

A continuous phytochemical study on the seeds of Senna obtusifolia (Syn.: Cassia obtusifolia) led to the isolation of a new anthraquinone analogue, obtusifolin-2-O-ß-D-(6'-O-α,ß-unsaturated butyryl)-glucopyranoside (1) and a new eurotinone analogue, epi-9-dehydroxyeurotinone-ß-D-glucopyranoside (2). Their structures were established mainly by NMR and MS experiments as well as the necessary chemical evidences. Their inhibitory effects on two organic anion transporters (OAT1 and OAT3) were investigated and the results showed that 1 exhibited a strongly specific inhibitory effect on OAT1 at 100 µM.


Assuntos
Antraquinonas/isolamento & purificação , Transportadores de Ânions Orgânicos/antagonistas & inibidores , Sementes/química , Senna (Planta)/química , Antraquinonas/química , Antraquinonas/farmacologia , Humanos , Estrutura Molecular , Proteína 1 Transportadora de Ânions Orgânicos/antagonistas & inibidores , Transportadores de Ânions Orgânicos Sódio-Independentes/antagonistas & inibidores
5.
J Asian Nat Prod Res ; 21(10): 970-976, 2019 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-29947250

RESUMO

A phytochemical study on the seeds of Cassia obtusifolia was carried out, which finally led to obtain two naphthalenes (1 and 2), two naphthopyrans (3 and 4) and twelve anthraquinones (5-16). The structures of all compounds were established mainly by NMR and MS experiments as well as the necessary chemical evidence. Among them, 1 and 2 (obtusinaphthalensides A and B) were identified to be new naphthalene glycosides.

6.
J Pharm Biomed Anal ; 156: 340-348, 2018 Jul 15.
Artigo em Inglês | MEDLINE | ID: mdl-29751287

RESUMO

As a traditional processing method, sulfuring has been used in the processing of many traditional Chinese medicines (TCMs). Desulfuring, which has emerged in recent years, is a new method applied to sulfured herbs so they can comply with regulations regarding residual SO2. Due to the chemical transformations and the residual SO2 in the herbs, both sulfuring and desulfuring have negative effects on the safety and therapeutic effects of TCMs, and Moutan Cortex is one of the TCMs most susceptible to these effects. Here, a new strategy was developed to differentiate normal, sulfured and desulfured Moutan Cortex, and the transformations of compounds in sulfuring and desulfuring processes were analyzed using ultra-performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry (UPLC/Q-TOF-MSE) method based on metabolomic analysis. Our findings were as follows: (1) a total of 119 compounds were identified or tentatively identified, including 9 compounds that are being reported for the first time as natural products; (2) 15 sulfocompounds were generated during the sulfuring process; (3) these sulfocompounds could not be converted back into their corresponding glycosides by the desulfuring process, and the desulfuring decreased the residual SO2,while also removing some soluble compounds in the sulfured Moutan Cortex; and (4) 28 compounds were screened and tentatively identified as markers for distinguishing normal, sulfured and desulfured Moutan Cortex. Our findings provide a new practical strategy for evaluating how sulfuring and desulfuring affect the quality of TCMs.


Assuntos
Composição de Medicamentos/métodos , Medicamentos de Ervas Chinesas/análise , Controle de Qualidade , Dióxido de Enxofre/química , Biomarcadores/análise , Biomarcadores/química , Cromatografia Líquida de Alta Pressão/métodos , Medicamentos de Ervas Chinesas/química , Glicosídeos/análise , Glicosídeos/química , Espectrometria de Massas/instrumentação , Espectrometria de Massas/métodos , Metabolômica/instrumentação , Metabolômica/métodos , Monoterpenos/análise , Monoterpenos/química , Paeonia/química , Raízes de Plantas/química
7.
Zhongguo Zhong Yao Za Zhi ; 43(5): 1062-1070, 2018 Mar.
Artigo em Chinês | MEDLINE | ID: mdl-29676109

RESUMO

Epimedii Folium has a long history in China as a common traditional Chinese medicine. Key factors of Epimedii Folium quality were summarized based on ancient literatures, Chinese Pharmacopoeias and modern research in different period of history. The main reason for unqualified Epimedii Folium is unstable icariin. Therefore, it's suggested that: the precondition of the quality control of epimedium is to find the proper quality marker. It's suggested that the medicinal parts should be reverted to "dry whole plant overground" to solve Epimedium resource shortage problem. In addition, it is necessary to strengthen the standardized cultivation, so as to ensure germplasm, production area, and producing method to guarantee the quality of Epimedium Folium. In the drying method, it is recommended to change "dry in the sun or shade" to "dry", namely dry in the sun, shade or drier, in order to provide a new method to improve the quality control and quality standard of Epimedii Folium.


Assuntos
Medicamentos de Ervas Chinesas/farmacologia , Medicamentos de Ervas Chinesas/normas , Epimedium/química , Flavonoides/química , China , Folhas de Planta/química
8.
Zhongguo Zhong Yao Za Zhi ; 43(6): 1077-1085, 2018 Mar.
Artigo em Chinês | MEDLINE | ID: mdl-29676111

RESUMO

Salviae Miltiorrhizae Radix et Rhizoma (Danshen) is one of the commonly used bulk medicinal materials in China. It is widely used in clinical practice, and has many pharmacological effects, such as antithrombotic,antibacterial,anti-inflammatory,anti-oxidative and immunomodulatory activities. The quality of Danshen determines the quality of the curative effect. The current wild resources of Danshen gradually decrease, the cultivation area is widely distributed, among them, Shandong, Sichuan, Anhui, Shanxi, Hebei, Henan and other provinces have large acreage for Danshen. However, germplasm, origin, cultivation, harvest, process and other factors have a certain impact on the active ingredients of Danshen, which lead to the quality of Danshen good and bad mixed. This paper is based on the systematic analysis of literature on quality evaluation of Danshen. To provide a new idea for the further research on the quality of Danshen, we summarized the main factors affecting the quality of Salvia miltiorrhiza that of germplasm, origin, cultivation, harvest, process, storage, product specifications and so on.


Assuntos
Medicamentos de Ervas Chinesas/normas , Salvia miltiorrhiza/química , China , Raízes de Plantas/química , Controle de Qualidade , Rizoma/química
9.
Zhongguo Zhong Yao Za Zhi ; 43(6): 1201-1208, 2018 Mar.
Artigo em Chinês | MEDLINE | ID: mdl-29676129

RESUMO

In this paper, an approach was applied for separation and identification of oligosaccharides in Morinda officinalis How by Ultra performance liquid chromatography/quadrupole time-of-flight mass spectrometry (UPLC-Q-TOF-MS) with collision energy. The separation was carried out on an ACQUITY UPLC BEH Amide C18(2.1mm×100 mm,1.7 µm) with gradient elution using acetonitrile(A) and water(B) containing 0.1% ammonia as mobile phase at a flow rate of 0.2 mL·min⁻¹. The column temperature was maintained at 40 °C. The information of accurate mass and characteristic fragment ion were acquired by MSE in ESI negative mode in low and high collision energy. The chemical structures and formula of oligosaccharides were obtained and identified by the software of UNIFI and Masslynx 4.1 based on the accurate mass, fragment ions, neutral losses, mass error, reference substance, isotope information, the intensity of fragments, and retention time. A total of 19 inulin oligosaccharide structures were identified including D(+)-sucrose, 1-kestose, nystose, 1F-fructofuranosyl nystose and other inulin oligosaccharides (DP 5-18). This research provided important information about the inulin oligosaccharides in M. officinalis. The results would provide scientific basis for innovative utilization of M. officinalis.


Assuntos
Inulina/análise , Morinda/química , Oligossacarídeos/análise , Cromatografia Líquida de Alta Pressão , Compostos Fitoquímicos/análise , Espectrometria de Massas em Tandem
10.
J Pharm Biomed Anal ; 148: 1-5, 2018 Jan 30.
Artigo em Inglês | MEDLINE | ID: mdl-28946041

RESUMO

The edible flower buds of honeysuckle are traditionally used as herbal medicine or food additives in China. Chlorogenic acid (CGA) serves as the quality control marker of honeysuckle for its high content and antipyretic property. In this paper, a specific monoclonal antibody (mAb2E2) against CGA was produced. After optimization, mAb2E2-based indirect competitive enzyme-linked immunosorbent assay (icELISA) was developed. The concentrations of CGA producing 50% inhibition and the calibration range of the icELISA were 0.39 and 0.10-1.51ng/mL, respectively. The icELISA had cross-reactivity with 3,5-Dicaffeoylquinic acid (17.53%), and the cross-reactivity levels with other analogs were all below 5%. The average recoveries obtained by standard CGA addition to honeysuckle samples were from 88.4 to 104.8%. The icELISA was applied to CGA detection in different honeysuckle samples and the results were confirmed by high-performance liquid chromatography analysis. The correlation coefficient between the two assays was 0.97. The proposed icELISA provides a feasible analytical method for highly sensitive and specific, simple, fast, and high-throughput determination of CGA in honeysuckle samples.


Assuntos
Anticorpos Monoclonais/química , Ácido Clorogênico/química , Lonicera/química , Plantas Medicinais/química , Animais , China , Cromatografia Líquida de Alta Pressão/métodos , Reações Cruzadas , Ensaio de Imunoadsorção Enzimática/métodos , Feminino , Flores/química , Camundongos , Camundongos Endogâmicos BALB C
11.
J Nat Med ; 72(1): 166-180, 2018 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-28914410

RESUMO

Twenty-three new C21 steroidal glycosides, marstenacissides C1-C10 (1-10), D1-D7 (11-17) and E1-E6 (18-23), and four new C21 steroids, 11α,12ß-O-ditigloyl-tenacigenin C (24), 11α-O-benzoyl-12ß-O-tigloyl-tenacigenin C (25), 11α-O-tigloyl-12ß-O-benzoyl-tenacigenin C (26) and 11α-O-tigloyl-12ß-O-benzoyl-marsdenin (27), were isolated from the Dai herbal medicine Dai-Bai-Jie, derived from the roots of Marsdenia tenacissima. The chemical structures of all compounds were established by spectroscopic techniques, including high-resolution mass spectrometry and NMR spectroscopy, as well as by comparison with reported spectral data. The anti-HIV activities of these compounds were screened, and the compounds obtained displayed inhibitory effects against HIV-1 with inhibition rates of 36.4-81.3% at 30 µM.


Assuntos
Infecções por HIV/tratamento farmacológico , Medicina Herbária/métodos , Marsdenia/química , Fitoterapia/métodos , Raízes de Plantas/química , Plantas Medicinais/química
12.
Zhongguo Zhong Yao Za Zhi ; 42(18): 3435-3442, 2017 Sep.
Artigo em Chinês | MEDLINE | ID: mdl-29218925

RESUMO

In this paper,the potential climate factors affecting the Pairs polyphylla var. yunnanensis distribution in China at rational scales were selected from related literatures, using the sampling point geographic information from of P. polyphylla var. yunnanensis, combine the maximum entropy model (MaxEnt) with spatial analyst function of ArcGIS software, to study the climate suitability of P. polyphylla var. yunnanensis cultivating region in China and the leading climate factors. The results showed that, average rainfall in August, average rainfall in October, coefficient of variation of seasonal precipitation, the average temperature of the dry season, isothermal characteristic, average temperature in July were the leading climate factors affecting the potential distribution of P. polyphylla var. yunnanensis cultivating region in China, with their cumulative contribution rate reached 97.2% of all candidate climate factors. Existence probability of the region to be predicted of P. polyphylla var. yunnanensis through the constructed model, the climate unsuitable region, low, medium and high region of P. polyphylla var. yunnanensis in China were clarified and the threshold of climatic factors were gave and clarified the climate characteristics of the cultivating region in each climatic suitability division. The results of research can provide reference for production layout and introduction of P. polyphylla var. yunnanensis.


Assuntos
Clima , Mineração de Dados , Liliaceae/crescimento & desenvolvimento , China , Medicamentos de Ervas Chinesas/análise , Liliaceae/química
13.
Zhongguo Zhong Yao Za Zhi ; 42(18): 3443-3451, 2017 Sep.
Artigo em Chinês | MEDLINE | ID: mdl-29218926

RESUMO

The study was established an HPLC method for simultaneous determination of 8 steroidal saponins (polyphyllins Ⅶ, H, Ⅵ, Ⅱ, Ⅰ, and Ⅴ, dioscin, and gracillin) in Paridis Rhizoma, and made an evaluation by determining steroidal saponins in 15 kinds of genus Paris. The analysis was performed on a Waters Acquity H-ClassTM UPLC ultrafine liquid chromatography system coupled with a PDA detector. The chromatographic separation was achieved through a CAPCELL PAK ADME (4.6 mm× 250 mm, 5 µm) column and the optimal mobile phase consisted of acetonitrile and water. The column was maintained at 21 ℃, and the flow rate was 0.8 mL•min ⁻¹. The UV detection wavelength was 203 nm. The results showed that ① the detected components can be well separated and all with good correlation coefficients. The standard calibration curves were linearly good (R2>0.999 9). The linearity was obtained over 0.041 70-3.812 00 µg. The average recoveries ranged from 95.91% to 103.8%. ② there are significant differences in the content of steroidal saponins from different species. The steroidal saponins are low content or almost none in P. mairei, P. polyphylla var. stenophylla, and P. delavayi have low content or almost did not contain, so these species are not suitable for medicinal use. The contents of steroidal saponins in P. polyphylla var. chinensis are varied from different places. There were high content of steroidal saponins in P. polyphylla var. yunnanensis, P. forrestii, P. daliensis, and P. axialis, even up to 5.0%, which indicated that they had the potential pharmic value of development.


Assuntos
Liliaceae/química , Fitosteróis/análise , Rizoma/química , Saponinas/análise , Cromatografia Líquida de Alta Pressão , Extratos Vegetais/química
14.
Zhongguo Zhong Yao Za Zhi ; 42(18): 3452-3460, 2017 Sep.
Artigo em Chinês | MEDLINE | ID: mdl-29218927

RESUMO

In order to study whether Paris forrestii could be developed as a substitute of Paridis Rhizome, chemical compositions of P. forrestii and P. polyphylla var. yunnanensis were investigated by UPLC-Q-TOF MS. In addition, the contents of eight primary steroidal saponins in 77 batches of P. forrestii samples from different habitats were simultaneously determined by HPLC-UV. The results showed that P. forrestii and P. polyphylla var. yunnanensis have similar chemical compositions, and all 22 major common peaks were identified as steroid derivatives. Meanwhile, there were some differences in the contents of saponins in P. forrestii samples from different habitats. The contents of 4 steroidal saponins in Chinese Pharmacopoeia ranged from 0.068% to 3.30%, and the highest content of the 8 kinds of steroidal saponins was 6.18%, while the lowest was just 0.71%. Moreover, 78% of P. forrestii samples were in conformity with the requirements of Chinese Pharmacopoeia, indicating that P. forrestii samples had relatively stable quality and could be further studied as a substitute for Paridis Rhizome.


Assuntos
Medicamentos de Ervas Chinesas/análise , Liliaceae/química , Saponinas/análise , Cromatografia Líquida de Alta Pressão , Ecossistema , Liliaceae/classificação , Compostos Fitoquímicos/análise , Rizoma
15.
Zhongguo Zhong Yao Za Zhi ; 42(18): 3461-3464, 2017 Sep.
Artigo em Chinês | MEDLINE | ID: mdl-29218928

RESUMO

Paris is a raw material of a variety of Chinese medicines, which has become deficient in resource due to market demand substantial growth and wild Paris resources reducing increasingly and the artificial cultivation slow growth. This study compared pharmacological activity in analgesia and anti-inflammatory and hemostasis effects of P. forrestii with pharmacopoeial Paridis Rhizoma to expand its range of Paris medicinal resources and protect wild resources of Paris and meet market demand. The experimental study showed that P. forrestii and P. polyphylla var. yunnanensis and P. polyphylla var. chinensis had analgesic, anti-inflammatory and hemostatic effects. They can significantly reduce the number of writhing and inhibit rat foot swelling induced by carrageenan and mice capillary permeability induced by acetic acid and short the bleeding time and clotting time. Their function is equivalent.


Assuntos
Liliaceae/química , Compostos Fitoquímicos/farmacologia , Rizoma/química , Analgésicos/farmacologia , Animais , Anti-Inflamatórios/farmacologia , Hemostáticos/farmacologia , Liliaceae/classificação , Camundongos , Ratos
16.
Zhongguo Zhong Yao Za Zhi ; 42(18): 3465-3468, 2017 Sep.
Artigo em Chinês | MEDLINE | ID: mdl-29218929

RESUMO

Paris is commonly used in traditional Chinese medicine and its resource is in shortage, a variety of related plants are acquired as Paris. This study compared pharmacological activity in anti-inflammatory and hemostatic and blood rheology of P. vietnamensis with pharmacopoeial Paridis Rhizoma to expand its range of Paris medicinal resources and protect wild resources of Paris and meet market demand. The experimental study showed that P. vietnamensis and P. polyphylla var. yunnanensis and P. polyphylla var. chinensis had anti-inflammatory and hemostatic effect and improved blood rheolog. They can significantly inhibit rat foot swelling induced by carrageenan and short the bleeding time and clotting time and reduce the blood viscosity in rats with acute blood stasis model, P. vietnamensis and P. polyphylla var. yunnanensis can inhibit mice capillary permeability induced by acetic acid.


Assuntos
Anti-Inflamatórios/farmacologia , Hemostáticos/farmacologia , Liliaceae/química , Compostos Fitoquímicos/farmacologia , Animais , Camundongos , Rizoma/química
17.
Zhongguo Zhong Yao Za Zhi ; 42(18): 3469-3473, 2017 Sep.
Artigo em Chinês | MEDLINE | ID: mdl-29218930

RESUMO

Based on the field investigation, this paper researched the germplasms and geoherbs habitat of Chonglou in ancient herbal books systematically. The results showed that, Chonglou in ancient herbal books sometimes referred to certain specific germplasm, while sometimes it referred to many species derived from genus Paris except Sect. Paris. The medicinal material Chonglou in Chinese Materia Medica Bencaotujing and Bencaomengquan was verified as P. polyphylla var. chinensis, which could be P. polyphylla in Xinxiubencao, and it should include P. polyphylla or P. polyphylla var. stenophylla in Bencaogangmu. However, it proved to be a variety of species from Paris that can used as Chonglou in Diannanbencao and Zhiwumingshitukao. Moreover, the origins of Chonglou were correspondingly more diverse, with its producing areas changed from North to South. Therefore, with the resources increasing endangered, the botanical origin of Chonglou should be further studied.


Assuntos
Herbários como Assunto , Liliaceae/química , China , Medicamentos de Ervas Chinesas/química , Pesquisa
18.
Zhongguo Zhong Yao Za Zhi ; 42(18): 3504-3508, 2017 Sep.
Artigo em Chinês | MEDLINE | ID: mdl-29218934

RESUMO

In order to evaluate the quality of Artemisia argyi from Qichun, Ningbo, Anguo and Nanyang, the contents of eupatilin and jaceosidin were determined by RP-HPLC. The determination was performed on Agilent Eclipse XDB-C18 (4.6 mm×250 mm, 5 µm) with mobile phase consisted of acetonitrile-0.2% phosphoric acid(35∶65) at the flow rate 1.0 mL•min ⁻¹. The detection wavelength was 350 nm and the column temperature was 25 ℃. The results showed that the amount of eupatilin and jaceosidin had a clear linear relationship in the range of 0.003-0.126 g•L ⁻¹ (r=0.999 9) and 0.005-0.200 g•L ⁻¹ (r=0.999 9), and the average recovery rates for them were 99.14% (n=6, RSD 1.2%) and 99.40% (n=6, RSD=0.73%), respectively. The results showed that RP-HPLC can be used for the quantification of eupatilin and jaceosidin in the folium of A. argyi. With this method, we found there was no significant difference of jaceosidin content within all the samples collected, but the content of eupatilin was significantly higher in samples from Qichun, Ningbo, Xiangyang and Nanyang, located in the south of Huaihe River compared with these from other areas.


Assuntos
Artemisia/química , Flavonoides/análise , Folhas de Planta/química , China , Cromatografia Líquida de Alta Pressão , Manuscritos como Assunto , Compostos Fitoquímicos/análise
19.
Zhongguo Zhong Yao Za Zhi ; 42(18): 3530-3536, 2017 Sep.
Artigo em Chinês | MEDLINE | ID: mdl-29218938

RESUMO

Sulfur-containing Anemarrhenae Rhizoma decoction pieces were prepared by using sulfur-fumigating procedure. The difference components before and after sulfur fumigation in Anemarrhenae Rhizoma were analyzed and on-line identified by UPLC-Q-TOF-MSE combined with UNIFI informatics platform, principal component analysis (PCA) and orthogonal partial least squares discriminant analysis (OPLS-DA) respectively. As a result, 16 major differences components were identified, and among them, 9 components were mainly from sulfur-fumigated samples. The main chemical markers in sulfur-fumigated Anemarrhenae Rhizoma were identified as the sulfite derivatives newly produced after sulfur-fumigating. Meanwhile, UPLC-Q-TOF-MSE was used to find the main chemical marker anemarrhena saponin BⅡ sulfite (m/z 983). By using this method, a rapid screening method for sulfur-fumigated Anemarrhenae Rhizoma was established. This was a convenient and accurate detection method for sulfur dioxide residue, and it can be used as an effective assistant method to control the quality of Anemarrhenae Rhizoma. At the same time, it was the first time to identify sulfited derivatives of steroidal saponins, and screen the sulfur-fumigated Anemarrhenae Rhizoma.


Assuntos
Anemarrhena/química , Medicamentos de Ervas Chinesas/química , Rizoma/química , Enxofre , Cromatografia Líquida de Alta Pressão , Fumigação , Compostos Fitoquímicos/análise , Saponinas/análise
20.
Zhongguo Zhong Yao Za Zhi ; 42(21): 4234-4245, 2017 Nov.
Artigo em Chinês | MEDLINE | ID: mdl-29271166

RESUMO

This study was to identify the chemical constituents of Schisandra propinqua, one herbal medicine of Yi nationality in China by using ultra performance liquid chromatography-quadrupole-time of flight-mass spectrometry (UPLC-Q-TOF/MSE). Acetonitrile- water containing 0.1% formic acid was used as the mobile phase for gradient elution. Data were collected under ESI negative mode and ESI positive mode, and then screened and verified by the software of UNIFI and Masslynx4.1. Based on the accurate mass, fragment ions, neutral losses, mass error, retention time, reference substance, isotope information, the intensity of fragments, as well as the previous reports, the known compounds were validated and identified. The chemical structures of the unknown components were identified according to exact molecular weight, MS fragment, chromatographic retention behavior, and characteristic fragments of known congener compounds. A total of 68 chemical components were identified from S. propinqua, including 3 flavonoids, 10 flavanols, 34 lignans compounds (including 20 dibenzocyclooctene lignans), 4 triterpenoids, 17 organic acid and other compounds. 37 compounds of them were found in S. propinqua for the first time, and one potential compound needed to be identified.


Assuntos
Medicamentos de Ervas Chinesas/química , Compostos Fitoquímicos/análise , Schisandra/química , China , Cromatografia Líquida de Alta Pressão , Espectrometria de Massas em Tandem
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