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MethodsX ; 8: 101415, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34430310


In the current work, a rapid and simple dispersive liquid-liquid microextraction method (DLLME) was used to determine Bisphenol A (BPA). High performance liquid chromatography with the photodiode-array detector (HPLC-DAD) coupled DLLME method was employed to analyze BPA in food samples packaged including cans, paper boxes, and glass jars. The calibration curve was obtained to be in the linear range 0.009-25 ngg-1 with a correlation coefficient of R2 = 0.9981. The mean relative standard deviations (RSDs) was of 5.2% (n = 3). The limit of detection (LOD) and the limit of quantification (LOQ) of the method were obtained to be 0.001 ngg-1 and 0.08 ng.g-1, respectively. In sum, this method presents:•A rapid, simple and efficient modified DLLME method was used to measure BPA in packaged foods.•The advantages of this method were low detection limit, fast preparation, and high BPA recovery.•The DLLME-HPLC method consists of low detection limit and high recoveries to determine BPA in samples.•The results indicated that DLLME -HPLC-DAD was an applied method to measure BPA in food samples.

Chem Biodivers ; 15(6): e1700562, 2018 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-29575789


Total flavonoid content (TFC) and cyanidin-3-glucoside (Cyd-3-glu) of seed and seed coat extract of 16 genotypes from five species of Carthamus were studied, and their major polyphenolic compounds and antioxidant activity of the seed coat extracts were determined using HPLC analysis and DPPH assay, respectively. Additionally, fatty acids composition of the seed oil was analyzed by GC. In general, TFC and Cyd-3-glu content of seed coat extracts were higher than those of seed extracts. A novel breeding line with black seed coat (named A82) depicted lower TFC (3.79 mg QUE/g DW) but higher Cyd-3-glu (24.64 mg/g DW) compared to the white and other seed-pigmented genotypes. DPPH radical scavenging activity showed a strong association with Cyd-3-glu content (r = 0.84), but no correlation with TFC (r = -0.32). HPLC analysis of seed coat extracts revealed that four compounds were dominant constituents including rutin (7.23 - 117.95 mg/100 g DW), apigenin (4.37 - 64.88 mg/100 g DW), quercetin (3.09 - 14.10 mg/100 g DW), and ferulic acid (4.49 - 30.41 mg/100 g DW). Interestingly, the genotype A82 with an appropriate polyunsaturated/saturated fatty acids index (5.46%) and a moderate linoleic fatty acid content (64.70%) had higher nutritional and pharmaceutical value than all the other genotypes.

Antioxidantes/farmacologia , Compostos de Bifenilo/antagonistas & inibidores , Carthamus/química , Ácidos Graxos/farmacologia , Picratos/antagonistas & inibidores , Extratos Vegetais/farmacologia , Polifenóis/farmacologia , Sementes/química , Antioxidantes/química , Antioxidantes/isolamento & purificação , Compostos de Bifenilo/metabolismo , Ácidos Graxos/química , Ácidos Graxos/isolamento & purificação , Picratos/metabolismo , Extratos Vegetais/química , Extratos Vegetais/isolamento & purificação , Polifenóis/química , Polifenóis/isolamento & purificação , Especificidade da Espécie
Braz. arch. biol. technol ; 60: e17160564, 2017. tab, graf
Artigo em Inglês | LILACS-Express | LILACS | ID: biblio-951443


ABSTRACT Safflower (Carthamus tinctorius L.) is an annual herbaceous plant, cultivated mainly for the seed which is used for edible oil extraction and bird feeding. This study was designed to evaluate the safety of a new pigmented variety of safflower (A82) seeds. The results showed that oral administration of A82 seeds significantly increased the body weight of male rats in a dose-dependent manner (p<0.05). Biochemical tests showed that A82 seeds significantly increased the serum levels of AST (Aspartate aminotransferase) (p<0.05), slightly reduced the serum levels of ALT (Alanine aminotransferase) and significantly reduced ALP (p<0.05) levels in a dose dependent manner. BUN (Blood Urea Nitrogen) and Cr (Creatinine) were not significantly changed in A82 seed treated groups. Also, testosterone levels were not significantly changed by administration of different doses of A82. However, Johnson scoring showed slightly decrease in experimental groups. No organ weight or histological changes were observed in liver, kidney, spleen, heart and brain of A82 seed treated animals. These results indicate that A82 seeds have not any toxic effects in Wistar rats. Future studies are required to clarify the exact mechanism by which A82 seeds alter AST levels and body weight in rat.

Appl Biochem Biotechnol ; 168(4): 770-84, 2012 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-22907514


To diminish the time required for some diagnostic assays including reverse transcription PCR (RT-PCR), reverse transcription loop-mediated isothermal amplification (RT-LAMP; due to mainly RNA extraction step) and also DAS-ELISA into a minimum level, an innovative immunocapture RT-LAMP (IC-RT-LAMP) and immunocapture reverse transcription (IC/RT-PCR) protocol on the basis of Potato Leafroll virus (PLRV) genome were used and optimized. In this regard, all six IC-RT-LAMP primers (i.e. F3, B3, FIP, BIP, LF and LB) together with IC/RT-PCR primers were designed on the basis of the highly conserved sequence (ORF3) of coat protein gene (GenBank accession number: U73777) of PLRV genome. Even though DAS-ELISA, IC/RT-PCR and IC-RT-LAMP assays could successfully detect positive infected plant samples, considering the time, safety, sensitivity, cost and simplicity, the last one was overall superior. Meanwhile, among five different visual dyes to accurately detect IC-RT-LAMP products, both hydroxynaphthol blue and GeneFinder™ could produce long stable colour change and brightness in a close tube-based approach to prevent cross-contamination risk, concluded eventually as the best ones. Altogether, as IC-RT-LAMP is sensitive, cost-effective, fairly user friendly and also can generate more accurate results than previous diagnostic procedures, we accordingly propose this colorimetric assay as a highly reliable alternative viral recognition system regarding PLRV recognition and probably other viral-based diseases.

Luteoviridae/isolamento & purificação , Imagem Molecular/métodos , Cor , Ensaio de Imunoadsorção Enzimática , Genoma Viral/genética , Luteoviridae/genética , Reação em Cadeia da Polimerase Via Transcriptase Reversa , Segurança