Your browser doesn't support javascript.
loading
Mostrar: 20 | 50 | 100
Resultados 1 - 16 de 16
Filtrar
Mais filtros










Base de dados
Intervalo de ano de publicação
1.
Braz J Biol ; 83: e247016, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34495153

RESUMO

Pheretima posthuma (Vaillant, 1868), a native earthworm of Pakistan and Southeast Asia, has wide utilization in vermicomposting and bioremediation process. In this study, P. posthuma coelomic fluid (PCF) and body paste (PBP) was evaluated as antibacterial agent against ampicillin (AMP) resistant five Gram positive and four Gram negative clinical isolates. The antibacterial effect of different doses (i.e. 25-100 µg/ml) of PCF and PBP along with AMP and azithromycin (AZM) (negative and positive controls, respectively) were observed through disc diffusion and micro-dilution methods. All nine clinical isolates were noticed as AMP resistant and AZM sensitive. Antibacterial effects of PCF and PBP were dose dependent and zone of inhibitions (ZI) against all clinical isolates were between 23.4 ± 0.92 to 0 ± 00 mm. The sensitivity profile of PCF and PBP against clinical isolates was noticed as 44.44 and 55.56%, respectively. Both PCF and PBP showed bacteriostatic (BTS) action against S. aureus, S. pyogenes, K. pneumonia, N. gonorrhoeae. Moreover, the cumulative BTS potential of PCF and PBP against all isolates was 66.67 and 55.56%, respectively. The MICs of PCF and PBP were ranged from 50-200 µg/ml against selected isolates. The bacterial growth curves indicated that PCF and PBP inhibited the growth of all isolates at their specific MIC concentrations. However, PBP has better antibacterial potential compared to PCF against selected isolates. Therefore, it is concluded that both PCF and PBP of P. posthuma possess antibacterial and BTS potential against ampicillin resistant clinical isolates. This organism might be considered as a second choice of antibacterial agents and can further be utilized in pharmaceutical industries for novel drug manufacturing by prospecting bioactive potential agents.


Assuntos
Oligoquetos , Staphylococcus aureus , Ampicilina/farmacologia , Animais , Antibacterianos/farmacologia , Testes de Sensibilidade Microbiana
2.
Braz J Biol ; 83: e247562, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34495155

RESUMO

To investigate the role of cow dung in soil reclamation and bio assimilation along with bio accumulation of heavy metals in earthworm (P. posthuma) (N=900) earthworms were used and treatment groups of CD-soil mixture of different proportion of cow dung were designed. Nonlethal doses of lead acetate and cadmium chloride were added in treatment groups. Mature P. posthuma were released in each experimental pot maintaining the favorable conditions. The pH, carbon, nitrogen, phosphorus, exchangeable cations, and heavy metal level of each mixture was evaluated. The results indicated that bio-assimilation of Pb and Cd by P. posthuma were significantly (P ˂ 0.01) higher in different soil-CD treatments compared to control. Highest bio-assimilation of both metals was observed in T1 of both groups (Pb = 563.8 mg/kg and Cd = 42.95 mg/kg). The contents of both metals were significantly (P ˂ 0.05) lowered in casting. The nutrient concentration in the final castings of all soil-CD treatments were also equally transformed from less or insoluble to more soluble and available for plants, except for carbon level which increased with CD proportion. It is concluded that cow dung as organic matter has a positive effect on soil reclamation and bio-assimilation of metals by P. posthuma.


Assuntos
Metais Pesados , Oligoquetos , Poluentes do Solo , Animais , Bioacumulação , Cádmio , Bovinos , Feminino , Metais Pesados/análise , Solo
3.
Heliyon ; 6(4): e03513, 2020 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-32346624

RESUMO

The research in electrochemical reduction of CO2 is shifting towards the discovery of new and novel materials. This study shows a new class of material, that of Ge-S-In chalcogenide glass, to be active for reduction of CO2 in aqueous solutions. Experiments were conducted with bulk and particle form of the material, yielding different product for each structural form. Faradaic efficiency of upto 15% was observed in bulk form for CO production while formic acid with up to 26.1 % faradaic efficiency was measured in powder form. Chalcogenide studies have focused primarily on the photoelectrochemical reduction however these results provide a strong merit for introducing metal in chalcogenide glass structures for electrochemical reduction of CO2. The activity for CO2 reduction and the change in product selectivity reflects that further efforts to improve the glass structures can be undertaken in order to increase the faradaic efficiency and selectivity of the products.

4.
Mol Psychiatry ; 23(4): 973-984, 2018 04.
Artigo em Inglês | MEDLINE | ID: mdl-28397838

RESUMO

Approximately 1% of the global population is affected by intellectual disability (ID), and the majority receive no molecular diagnosis. Previous studies have indicated high levels of genetic heterogeneity, with estimates of more than 2500 autosomal ID genes, the majority of which are autosomal recessive (AR). Here, we combined microarray genotyping, homozygosity-by-descent (HBD) mapping, copy number variation (CNV) analysis, and whole exome sequencing (WES) to identify disease genes/mutations in 192 multiplex Pakistani and Iranian consanguineous families with non-syndromic ID. We identified definite or candidate mutations (or CNVs) in 51% of families in 72 different genes, including 26 not previously reported for ARID. The new ARID genes include nine with loss-of-function mutations (ABI2, MAPK8, MPDZ, PIDD1, SLAIN1, TBC1D23, TRAPPC6B, UBA7 and USP44), and missense mutations include the first reports of variants in BDNF or TET1 associated with ID. The genes identified also showed overlap with de novo gene sets for other neuropsychiatric disorders. Transcriptional studies showed prominent expression in the prenatal brain. The high yield of AR mutations for ID indicated that this approach has excellent clinical potential and should inform clinical diagnostics, including clinical whole exome and genome sequencing, for populations in which consanguinity is common. As with other AR disorders, the relevance will also apply to outbred populations.


Assuntos
Consanguinidade , Deficiência Intelectual/genética , Adulto , Mapeamento Cromossômico/métodos , Variações do Número de Cópias de DNA , Família , Feminino , Genes Recessivos , Heterogeneidade Genética , Homozigoto , Humanos , Deficiência Intelectual/metabolismo , Irã (Geográfico) , Mutação com Perda de Função , Masculino , Análise em Microsséries/métodos , Pessoa de Meia-Idade , Mutação , Paquistão , Linhagem , Sequenciamento Completo do Exoma/métodos
5.
Eur J Clin Nutr ; 65(6): 690-5, 2011 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-21386858

RESUMO

BACKGROUND/OBJECTIVES: Plasma leptin and adiponectin, and membrane phospholipid fatty acid composition are implicated into the mechanism of insulin resistance but no clear pattern has emerged. Hence, this study examined these variables in subjects presenting to the diabetic clinic for a diagnostic glucose tolerance test. SUBJECTS/METHODS: Body composition, glucose, glycated hemoglobin, insulin, leptin, adiponectin, and red cell and plasma phospholipid fatty acids were assessed from 42 normal and 28 impaired glucose tolerant subjects. Insulin sensitivity was determined by homeostatic model assessment. RESULTS: The plasma phosphatidylcholine fatty acid composition of the impaired glucose tolerant subjects was similar to that of normal subjects. However, the impaired glucose tolerant subjects had significantly lower linoleic (P<0.05), eicosapentaenoic (P<0.05) and docosahexaenoic (P<0.01) acids in the red cell phosphatidylcholine and phosphatidylethanolamine compared with the normal subjects. Moreover, red cell phosphatidylcholine docosahexaenoic acid correlated positively with adiponectin (r=0.290, P<0.05) but negatively with leptin (r=-0.252, P<0.05), insulin (r=-0.335, P<0.01) and insulin resistance (r=-0.322, P<0.01). Plasma triglycerides, leptin and glucose combined predicted about 60% of variation in insulin level whereas insulin was the only component that predicted the membrane fatty acids. CONCLUSIONS: We postulate that membrane phospholipids fatty acids have an indirect role in determining insulin concentration but insulin has a major role in determining membrane fatty acid composition.


Assuntos
Adipocinas/sangue , Membrana Celular/metabolismo , Eritrócitos/metabolismo , Ácidos Graxos/sangue , Intolerância à Glucose/sangue , Resistência à Insulina , Insulina/sangue , Adiponectina/sangue , Adulto , Glicemia/metabolismo , Ácidos Docosa-Hexaenoicos/sangue , Ácidos Graxos Ômega-3/sangue , Feminino , Glicerofosfolipídeos/sangue , Humanos , Leptina/sangue , Ácido Linoleico/sangue , Masculino , Pessoa de Meia-Idade , Triglicerídeos/sangue
6.
Cell Death Differ ; 15(6): 1073-83, 2008 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-18259196

RESUMO

Mitochondrial proteins such as cytochrome c, Smac/DIABLO and Omi/HtrA2 play important roles in the cell death pathways of mammalian cells. In Drosophila, the role of mitochondria in cell death is less clear. Here, we report the identification and characterization of the Drosophila ortholog of human Omi/HtrA2. We show that Drosophila Omi/HtrA2 is imported into the mitochondria where it undergoes proteolytic maturation to yield two isoforms, dOmi-L and dOmi-S. dOmi-L contains a canonical N-terminal IAP-binding motif (AVVS), whereas dOmi-S contains a distinct N-terminal motif (SKMT). DIAP1 was able to bind to both isoforms via its BIR1 and BIR2 domains. This resulted in cleavage of the linker region of DIAP1 between the BIR1 and BIR2 domains and further degradation of the BIR1 domain by the proteolytic activity of dOmi. The binding of DIAP1 to dOmi also resulted in DIAP1-mediated polyubiquitination of dOmi, suggesting that DIAP1 could target dOmi for proteasomal degradation. Consistent with this, expression of DIAP1 in Drosophila eye discs protected them from dOmi-induced eye ablation, indicating that DIAP1 plays an important role in protecting cells from the potentially lethal effects of dOmi. The ability of IAPs to bind to and ubiquitinate mitochondrial proteins such as dOmi may be a key conserved function to counterbalance the lethal effects of these proteins if accidentally released into the cytosol.


Assuntos
Apoptose , Proteínas de Drosophila/metabolismo , Drosophila/enzimologia , Proteínas Inibidoras de Apoptose/metabolismo , Proteínas Mitocondriais/metabolismo , Serina Endopeptidases/metabolismo , Motivos de Aminoácidos , Animais , Caspases/metabolismo , Citosol/enzimologia , Drosophila/citologia , Drosophila/crescimento & desenvolvimento , Proteínas de Drosophila/antagonistas & inibidores , Proteínas de Drosophila/química , Olho/citologia , Olho/enzimologia , Olho/crescimento & desenvolvimento , Serina Peptidase 2 de Requerimento de Alta Temperatura A , Proteínas Mitocondriais/análise , Proteínas Mitocondriais/antagonistas & inibidores , Proteínas Mitocondriais/química , Isoformas de Proteínas/química , Isoformas de Proteínas/metabolismo , Serina Endopeptidases/química , Ubiquitinação
7.
Child Care Health Dev ; 30(2): 103-11, 2004 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-14961863

RESUMO

BACKGROUND: Siblings of children with cancer are at risk for reduced emotional support. The role of emotional social support in the psychological adjustment of siblings of paediatric cancer patients was examined, in the context of age and gender. METHODS: The sample consisted of two groups of siblings of children being treated for cancer: siblings referred for behaviour problems (n = 47) and a comparison group of non-referred siblings (n = 25). Forty-two were female, and 30 were male. The mean age was 10.31 years (SD = 2.71). Siblings completed measures of depression, anxiety, behaviour, and emotional social support. One parent of each sibling completed measures of sibling's behaviour and anxiety. RESULTS: Siblings who reported more social support endorsed significantly fewer symptoms of depression, anxiety, and fewer behaviour problems, and their parents reported less anxiety and fewer behaviour problems than siblings who reported lower social support. Parents of referred siblings reported significantly more behaviour problems than parents of non-referred siblings. Referred adolescent females reported significantly higher depression scores and were perceived as more anxious than referred adolescent males and non-referred adolescent females. Non-referred younger siblings with high social support were perceived by their parents as having the fewest behaviour problems. CONCLUSIONS: High level of social support appears to play a protective role in psychological adjustment of siblings of paediatric cancer patients, with age and gender as modifying factors. Although not all siblings develop behaviour or emotional problems, it is critical to identify those who do in order to intervene accordingly.


Assuntos
Emoções , Neoplasias/psicologia , Irmãos/psicologia , Apoio Social , Adaptação Psicológica , Adolescente , Comportamento do Adolescente/psicologia , Fatores Etários , Ansiedade/psicologia , Criança , Transtornos do Comportamento Infantil/psicologia , Depressão/psicologia , Feminino , Humanos , Masculino , Neoplasias/terapia , Pais/psicologia , Autoavaliação (Psicologia) , Fatores Sexuais
8.
Virology ; 230(2): 309-22, 1997 Apr 14.
Artigo em Inglês | MEDLINE | ID: mdl-9143287

RESUMO

The replicase gene of the coronavirus MHV-A59 encodes a serine-like proteinase similar to the 3C proteinases of picornaviruses. This proteinase domain is flanked on both sides by hydrophobic, potentially membrane-spanning, regions. Cell-free expression of a plasmid encoding only the 3C-like proteinase (3CLpro) resulted in the synthesis of a 29-kDa protein that was specifically recognized by an antibody directed against the carboxy-terminal region of the proteinase. A protein of identical mobility was detected in MHV-A59-infected cell lysates. In vitro expression of a plasmid encoding the 3CLpro and portions of the two flanking hydrophobic regions resulted in inefficient processing of the 29-kDa protein. However, the efficiency of this processing event was enhanced by the addition of canine pancreatic microsomes to the translation reaction, or removal of one of the flanking hydrophobic domains. Proteolysis was inhibited in the presence of N-ethylmaleimide (NEM) or by mutagenesis of the catalytic cysteine residue of the proteinase, indicating that the 3CLpro is responsible for its autoproteolytic cleavage from the flanking domains. Microsomal membranes were unable to enhance the trans processing of a precursor containing the inactive proteinase domain and both hydrophobic regions by a recombinant 3CLpro expressed from Escherichia coli. Membrane association assays demonstrated that the 29-kDa 3CLpro was present in the soluble fraction of the reticulocyte lysates, while polypeptides containing the hydrophobic domains associated with the membrane pelletes. With the help of a viral epitope tag, we identified a 22-kDa membrane-associated polypeptide as the proteolytic product containing the amino-terminal hydrophobic domain.


Assuntos
Membrana Celular/virologia , Cisteína Endopeptidases/metabolismo , Vírus da Hepatite Murina/enzimologia , Proteínas Virais , Sequência de Aminoácidos , Animais , Linhagem Celular , Cisteína Endopeptidases/genética , Escherichia coli/metabolismo , Dados de Sequência Molecular , Processamento de Proteína Pós-Traducional , Coelhos , Proteínas Recombinantes de Fusão/genética , Proteínas Recombinantes de Fusão/metabolismo
9.
J Steroid Biochem ; 19(5): 1657-60, 1983 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-6227775

RESUMO

The sulphation of a number of contraceptive steroids by rabbit tissue in vitro was investigated. With liver tissue the three synthetic gestagens (norethisterone, norgesterel and lynestrenol) were sulphated at different rates and none was sulphated as rapidly as dehydroepiandrosterone; sulphation occurred at the tertiary 17 beta-hydroxyl group. The synthetic oestrogen ethynyloestradiol was sulphated more rapidly than dehydroepiandrosterone, both mono and disulphates being formed. Of the other tissues studied, sulphation occurred with stomach and lung but not with heart, spleen, muscle, kidney or adipose tissue. These in vitro studies provide confirmation of in vivo findings regarding sulphate conjugates of the synthetic steroids.


Assuntos
Anticoncepcionais Orais , Congêneres do Estradiol/metabolismo , Sulfatos/metabolismo , Animais , Radioisótopos de Carbono , Desidroepiandrosterona/metabolismo , Etinilestradiol/metabolismo , Feminino , Linestrenol/metabolismo , Masculino , Noretindrona/metabolismo , Norgestrel/metabolismo , Coelhos , Relação Estrutura-Atividade
10.
J Steroid Biochem ; 19(2): 1169-72, 1983 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-6887923

RESUMO

The in vitro metabolism of stereo-isomers (D, L and the racemic mixture DL) of norgestrel by a microsomal fraction from rabbit liver was investigated. The metabolism of the biologically active D-norgestrel was more rapid than that of L-norgestrel which is biologically inactive. This was mainly due to the more ready conversion of D-norgestrel to ring-A reduced metabolites. There was no difference between the two isomers in respect of the amount undergoing hydroxylation; about 40% of each isomer was converted to hydroxylated metabolites after 30 min incubation. However, there were differences between the isomers, D-norgestrel being converted mainly to the 16 beta-hydroxysteroid and L-norgestrel to the 16 alpha-hydroxysteroid. Similar amounts of both isomers were hydroxylated at C-6. The metabolism of the racemic mixture was intermediate between that of the D and L isomers.


Assuntos
Microssomos Hepáticos/metabolismo , Norgestrel/metabolismo , Animais , Feminino , Hidroxilação , Técnicas In Vitro , Coelhos , Estereoisomerismo , Testosterona/metabolismo
11.
Biochem J ; 197(1): 7-22, 1981 Jul 01.
Artigo em Inglês | MEDLINE | ID: mdl-6274326

RESUMO

Plasma-membrane and soluble fractions containing human chorionic gonadotropin/lutropin receptor were prepared from bovine corpora lutea by ultracentrifugation. The plasma-membrane and soluble fractions were studied for physicochemical properties, salts and gangliosides. The receptor preparations obtained from the plasma-membrane purified individually by sucrose-density-gradient centrifugation, which resulted in a partial dissociation of the hormone-binding subunit from the intact functional receptor unit, which consists of both hormone-binding (regulatory) and adenylate cyclase-associated (catalytic) subunits. The fractions containing the functional receptor unit were further purified by gel filtration on Sepharose-6B and chromatography on concanavalin A-Sepharose. The 'receptor' was finally purified by affinity chromatography on a column of controlled-pore glass covalently coupled to hu man chorionic gonadotropin. The purified receptor from the plasma-membrane and the soluble fractions contained binding capacities of 901000 and 87000 fmol of human chorionic gonadotropin/mg of protein. Yields of 0.02 and 0.22mg of protein were obtained from 250 g of bovine corpora lutea, which represents a 10000- and 1000-fold increase respectively in the specific binding with 125I-labelled human chorionic gonadotropin. Immunization of rabbits with a partially purified receptor fraction generated antibodies that specifically inhibited the binding of the 125I-labelled human chorionic gonadotropin to the receptor.


Assuntos
Corpo Lúteo/análise , Receptores de Superfície Celular/metabolismo , Animais , Bovinos , Fracionamento Celular , Membrana Celular/análise , Gonadotropina Coriônica/isolamento & purificação , Gonadotropina Coriônica/metabolismo , Cromatografia em Gel , Enzimas/farmacologia , Feminino , Gangliosídeos/farmacologia , Humanos , Hormônio Luteinizante/isolamento & purificação , Hormônio Luteinizante/metabolismo , Receptores de Superfície Celular/isolamento & purificação , Receptores do LH , Sais/farmacologia
SELEÇÃO DE REFERÊNCIAS
DETALHE DA PESQUISA
...