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1.
Medicina (Kaunas) ; 57(3)2021 Mar 16.
Artigo em Inglês | MEDLINE | ID: mdl-33809673

RESUMO

Endodontic microsurgery is a highly predictable treatment option in most cases when conventional endodontic treatment is not feasible. Nevertheless, mandibular molars are still considered by clinicians to be the most difficult type of teeth, with the lowest success rate. In recent years, endodontic microsurgery has been attempted more frequently with the emergence of modern cutting-edge technologies such as dental operating microscopes, various microsurgical instruments, and biocompatible materials, and the success rate is increasing. This review describes the current state of the art in endodontic microsurgical techniques and concepts for mandibular molars. Notably, this review highlights contemporary equipment, technology, and materials.

2.
J Am Dent Assoc ; 2020 Dec 05.
Artigo em Inglês | MEDLINE | ID: mdl-33293029

RESUMO

BACKGROUND AND OVERVIEW: Orthodontic miniscrews have become popular tools for providing temporary anchorage during orthodontic treatment. Although they are easy to insert, damage to the periodontal ligament or dental root during insertion is an unfavorable iatrogenic complication. Root perforation during miniscrew insertion in human teeth has been reported in a few articles. In this article, the authors describe the spontaneous repair of an iatrogenic root perforation in a mandibular first molar that occurred during insertion of an orthodontic miniscrew in a young girl undergoing orthodontic treatment. CASE DESCRIPTION: A 15-year-old girl with malocclusion was undergoing orthodontic treatment when the mesial root of her mandibular right first molar was damaged by an orthodontic miniscrew. The miniscrew and corresponding bracket were immediately removed to avoid any unnecessary forces on the tooth. Because the pulp remained vital without any additional damage and infection for 6 months, orthodontic treatment was resumed and completed in 9 months without any pulp damage or unfavorable symptoms. Serial periapical radiographs and cone-beam computed tomographic images showed that the injured area was surrounded by reparative tissue without any apical lesion. CONCLUSIONS AND PRACTICAL IMPLICATIONS: The findings of this case suggest that immediate removal of unnecessary forces provides an environment for spontaneous repair in cases of iatrogenic root perforation by orthodontic miniscrews, even when the damage involves the pulp. If force stimulation is avoided for a certain period, which was 6 months in this case, it may be possible to complete the orthodontic treatment without unfavorable symptoms.

3.
J Clin Med ; 9(11)2020 Nov 19.
Artigo em Inglês | MEDLINE | ID: mdl-33228002

RESUMO

This study aimed to evaluate one-year radiographic healing after endodontic microsurgery using CBCT with modified PENN 3D criteria and to compare the outcome with results evaluated using Molven's criteria. A total of 107 teeth from 96 patients were evaluated one year after endodontic microsurgery by using CBCT scans with modified PENN 3D criteria and periapical radiographs with Molven's criteria. Both preoperative and postoperative lesion volumes were calculated using ITK-SNAP (free software). Radiographic healing assessment using periapical radiographs and CBCT images, and preoperative and postoperative lesion volume measurements were performed independently by two examiners. The assessment using Molven's criteria resulted in 75 complete healings, 18 incomplete healings, eight uncertain healings, and six unsatisfactory healings. Based on modified PENN 3D criteria, 64 teeth were categorized as complete healing, 29 teeth as limited healing, six teeth as uncertain healing, and eight teeth as unsatisfactory healing. With the one-year follow-up, CBCT scans showed a lower healing tendency than did periapical radiography. The volumes of apical radiolucency after the surgery were reduced by 77.7% on average at one-year follow up.

4.
Materials (Basel) ; 13(22)2020 Nov 20.
Artigo em Inglês | MEDLINE | ID: mdl-33233519

RESUMO

The aim of this study was to evaluate the biocompatibility of calcium silicate-based sealers (CeraSeal and EndoSeal TCS) and epoxy resin-based sealer (AH-Plus) in terms of cell viability, inflammatory response, expression of mesenchymal phenotype, osteogenic potential, cell attachment, and morphology, of human periodontal ligament stem cells (hPDLSCs). hPDLSCs were acquired from the premolars (n = 4) of four subjects, whose ages extended from 16 to 24 years of age. Flow cytometry analysis showed stemness of hPDLSCs was maintained in all materials. In cell viability test, AH-Plus showed the lowest cell viability, and CeraSeal showed significantly higher cell viability than others. In ELISA test, AH-Plus showed higher expression of IL-6 and IL-8 than calcium silicate-based sealers. In an osteogenic potential test, AH-Plus showed a lower expression level than other material; however, EndoSeal TCS showed a better expression level than others. All experiments were repeated at least three times per cell line. Scanning electronic microscopy studies showed low degree of cell proliferation on AH-Plus, and high degree of cell proliferation on calcium silicate-based sealers. In this study, calcium silicate-based sealers appear to be more biocompatible and less cytotoxic than epoxy-resin based sealers.

5.
Restor Dent Endod ; 45(3): e27, 2020 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-32839708

RESUMO

Apical surgery for a mandibular molar is still challenging for many reasons. This report describes the applications of computer-guided cortical 'bone-window technique' using piezoelectric saws that prevented any nerve damage in performing endodontic microsurgery of a mandibular molar. A 49-year-old woman presented with gumboil on tooth #36 (previously endodontically treated tooth) and was diagnosed with chronic apical abscess. Periapical lesions were confirmed using cone-beam computed tomography (CBCT). Endodontic microsurgery for the mesial and distal roots of tooth #36 was planned. Following the transfer of data of the CBCT images and the scanned cast to an implant surgical planning program, data from both devices were merged. A surgical stent was designed, on the superimposed three-dimensional model, to guide the preparation of a cortical window on the buccal side of tooth #36. Endodontic microsurgery was performed with a printed surgical template. Minimal osteotomy was required and preservation of the buccal cortical plate rendered this endodontic surgery less traumatic. No postoperative complications such as mental nerve damage were reported. Window technique guided by a computer-aided design/computer-aided manufacture based surgical template can be considerably useful in endodontic microsurgery in complicated cases.

6.
J Endod ; 46(6): 872-880, 2020 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-32312482

RESUMO

Endodontic microsurgery for a tooth with a large periapical lesion and an intact cortical plate may necessitate the removal of extra bone and cause delayed or unfavorable healing. In such cases, the "bone window" technique offers excellent exposure to the operative field and preserves cortical bone without placing any additional graft material. In the reported cases, root-end surgery was performed on the maxillary and mandibular molars with a large periapical lesion. The bone window was fashioned with the aid of thin osteotomy instruments and repositioned to the original site at the end of the procedure, which resulted in minimizing bone loss and acted as an autologous graft for the surgical site. At 12- and 16-month follow-ups, clinical examinations and cone-beam computed tomographic scans revealed the healing of the lesion without symptoms or complications. Cone-beam computed tomographic imaging was used as a presurgical assessment tool for indication selection and precise design of the bone window. The use of a bone window in endodontic microsurgery, which appears to be a reliable technique, should be the method of preference when the large lesion is deeply positioned between intact buccal and lingual cortices.


Assuntos
Tomografia Computadorizada de Feixe Cônico , Microcirurgia , Osso Cortical , Maxila , Dente Molar
7.
J Endod ; 46(4): 539-544, 2020 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-32067720

RESUMO

INTRODUCTION: The aim of this study was to investigate the influence of apical filling material and the modification made to the apical preparation design in surgical endodontics on the areas of stress concentration in the mesial root of a mandibular molar using finite element analysis. METHODS: The filling material was injected under 2 conditions (ie, with or without mineral trioxide aggregate retrograde filling). The apical preparation design was modified by extending the preparation mesially while maintaining a similar prepared area. We contained the displacement of all the nodes at the base of the supporting bone and applied a force of 150 N to the vertical axis. We analyzed stress generation and concentrations numerically for all cavity design groups. RESULTS: In the presence of retrograde filling, the von Mises stress decreased gradually according to the enlargement of the prepared cavity in the subgroups. When the retrograde filling was absent, the von Mises stress increased as the prepared cavity enlarged. The modification of the apical preparation extending in the mesial direction showed a drastic decrease in stress concentration. CONCLUSIONS: Within the limitations of this study, it was advantageous to perform mesial retrograde preparation within the mesial root dentin to maintain a balanced root dentin on both sides of the apical preparation and create a low-stress field. The surgeon should be careful not to wash out or dislodge the retrograde filling material during obturation to avoid failure of surgery.


Assuntos
Endodontia , Dente Molar , Preparo da Cavidade Dentária , Análise de Elementos Finitos , Raiz Dentária
8.
J Clin Med ; 9(2)2020 Jan 28.
Artigo em Inglês | MEDLINE | ID: mdl-32012982

RESUMO

: This study was performed to investigate the changes in gene expression in periodontal ligament (PDL) cells following mechanical stimulus through RNA sequencing. In this study, premolars extracted for orthodontic treatment were used. To stimulate the PDL cells, an orthodontic force of 100× g was applied to the premolar (experimental group; n = 11), whereas the tooth on the other side was left untreated (control group; n = 11). After the PDL cells were isolated from the extracted teeth, gene set enrichment analysis (GSEA), differentially expressed gene (DEG) analysis, and real-time PCR were performed to compare the two groups. GSEA demonstrated that gene sets related to the cell cycle pathway were upregulated in PDL. Thirteen upregulated and twenty downregulated genes were found through DEG analysis. Real-time PCR results confirmed that five upregulated genes (CC2D1B, CPNE3, OPHN1, TANGO2, and UAP-1) and six downregulated genes (MYOM2, PPM1F, PCDP1, ATP2A1, GPR171, and RP1-34H18.1-1) were consistent with RNA sequencing results. We suggest that, from among these eleven genes, two upregulated genes, CPNE3 and OPHN1, and one downregulated gene, PPM1F, play an important role in PDL regeneration in humans when orthodontic force is applied.

9.
Sci Rep ; 10(1): 1247, 2020 01 27.
Artigo em Inglês | MEDLINE | ID: mdl-31988399

RESUMO

The purpose of this study was to evaluate the incidence and microscopic anatomy of the isthmus to provide more precise anatomical information about the mesiobuccal (MB) roots of the maxillary first molars and the mesial (M) roots of the mandibular first molars. Twenty-eight maxillary and 31 mandibular first molars were embedded, sectioned, stained, and observed at 30× magnification to evaluate the incidence and microscopic anatomy of the isthmus. The incidence of an isthmus 3 mm from the apex was 89.3% and 100% in the MB roots of the maxillary first molars and in the M roots of the mandibular first molars, respectively. The mean dentin thickness between the isthmus and the distal root surface was <1 mm at a distance of 3 mm from the apex in both types of roots. In this study, whenever two main canals were located in the MB roots of the maxillary first molars and in the M roots of the mandibular first molars, the likelihood of the presence of an isthmus increased. Therefore, clinicians should be aware of the thinnest dimensions in the distal surface of the MB roots of the maxillary first molars and the M roots of the mandibular first molars during nonsurgical and surgical root canal treatment.


Assuntos
Cavidade Pulpar/anatomia & histologia , Dente Molar/anatomia & histologia , Raiz Dentária/anatomia & histologia , Adulto , Dentina/anatomia & histologia , Feminino , Humanos , Masculino , Microscopia/métodos , Odontometria , República da Coreia/epidemiologia , Ápice Dentário/anatomia & histologia
10.
BMC Oral Health ; 20(1): 23, 2020 01 29.
Artigo em Inglês | MEDLINE | ID: mdl-31996198

RESUMO

BACKGROUND: This study, by using a variable-controlled survey model, sought to compare clinical decisions made by dentists with different clinical backgrounds in South Korea regarding teeth with apical periodontitis and to identify factors that influenced decision-making. METHODS: A questionnaire with 36 questions about identical patient information, clinical signs, and symptoms was filled out by participants. Each question referred to a radiograph that had been manipulated using computer software in order to control tooth-related factors. Participants were instructed to record their demographic information and choose the ideal treatment option related to each radiograph. Simple and multivariable logistic regression analyses (p < .05) were used to investigate factors related to the decision to extract the tooth. We divided factors into dentist-related factors (gender, years of experience, and professional registration) and tooth-related factors (tooth position, coronal status, root canal filling status, and size of the periapical radiolucency). Dentists were categorized into three groups, based on professional registration: general dental practitioners (GDPs), endodontists, and other specialists. Simple logistic regression analysis (p < .05) was used to evaluate the tooth-related factors influencing extraction, depending on the dentists' specialty. RESULTS: Participants mostly preferred saving the teeth over extraction. This preference was highest among the endodontists, followed by other specialists and GDPs. Extractions were significantly preferred for molars, teeth with previous root canal fillings, and those with apical lesions greater than 5 mm. CONCLUSIONS: This study suggests that dentists' decision-making regarding teeth with apical periodontitis was associated with their work experience and specialty and influenced by tooth position, root canal filling status, and size of the apical lesion. CLINICAL RELEVANCE: This survey revealed that clinical decision-making related to teeth with apical periodontitis was affected by dentists' specialty and work experience and by tooth-related factors, such as tooth position, root canal filling status, and size of the apical lesion.


Assuntos
Tomada de Decisão Clínica/métodos , Odontólogos/psicologia , Periodontite Periapical , Padrões de Prática Odontológica/estatística & dados numéricos , Tratamento do Canal Radicular , Adulto , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Periodontite Periapical/diagnóstico , Periodontite Periapical/terapia , República da Coreia , Inquéritos e Questionários
11.
J Endod ; 46(1): 97-102, 2020 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-31759678

RESUMO

INTRODUCTION: The aim of this study was to compare the volume of voids between retrograde filling using calcium silicate cement alone and retrograde filling using a combination of calcium silicate cement with a calcium silicate-based sealer. METHODS: Twenty single-rooted, extracted human teeth were instrumented with nickel-titanium files and obturated with gutta-percha. We resected the roots at a point 3 mm from the apex, prepared the root ends, and filled the root-end cavities with Endocem Zr (Maruchi, Wonju, Korea) or Endoseal MTA (Maruchi) plus Endocem Zr. Then, we scanned the prepared samples using micro-computed tomographic imaging and performed 3-dimensional reconstruction. The percentage volume of the gap between the canal wall and root-end filling was calculated along with the percentage volume of voids in the filling materials. All data were analyzed using the Mann-Whitney U test. Selected specimens were further observed using scanning electron microscopy. RESULTS: There were no significant differences in the percentage volumes of the gaps and internal voids between the 2 groups. Both calcium silicate and the calcium silicate-based sealer exhibited good adaptation to the cavity wall. CONCLUSIONS: The findings of our micro-computed tomographic and scanning electron microscopic analyses suggested that the volume of voids in retrograde filling using a combination of calcium silicate cement and calcium silicate-based sealer was comparable with that in retrograde filling using calcium silicate cement only. Calcium silicate-based sealers could be used in retrograde filling for clinical convenience without harmful effects on the quality of filling.


Assuntos
Compostos de Cálcio , Materiais Restauradores do Canal Radicular , Cimento de Silicato , Silicatos , Cálcio , Cavidade Pulpar , Guta-Percha , Humanos , Teste de Materiais , Obturação do Canal Radicular
12.
Clin Oral Investig ; 24(1): 247-255, 2020 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-31081517

RESUMO

OBJECTIVES: The purpose of this retrospective study was to evaluate and compare the effects of fast- and slow-setting calcium silicate-based materials (CSMs) used for root-end filling on the outcome of endodontic microsurgery. MATERIALS AND METHODS: We searched a clinical database for patients who had received endodontic microsurgery between 2001 and 2016. Included cases were divided into two groups according to the type of CSM used for root-end filling: slow-setting CSM (SCSM) and fast-setting CSM (FCSM). The cases in two groups were subjected to 1:1 nearest neighbor propensity score matching for the following variables: age, sex, tooth type, quality of canal filling, lesion type, and postoperative restoration. For each matched case, the outcome was determined as success or failure according to clinical and radiographic evaluations performed at least 1 year after surgery. Multivariate logistic regression analysis was performed to identify prognostic factors and estimate their effects. RESULTS: In total, 304 cases of endodontic microsurgery (179 SCSM and 125 FCSM) were identified, and 1:1 propensity score matching finally included 122 cases from each group. After matching, all covariates were associated with an absolute standardized difference of < 0.1. The overall success rates were 85.2% and 93.4% for the SCSM and FCSM groups, respectively (p = 0.062). Age, tooth type, lesion type, and CSM type were significantly associated with the outcome of endodontic microsurgery (p < 0.05). CONCLUSIONS: Within the limitations, the outcome of endodontic microsurgery using FCSMs was comparable with that of SCSMs. The findings suggest that the type of CSM used for root-end filling, particularly in terms of the initial setting time, could affect the outcome of endodontic microsurgery. CLINICAL RELEVANCE: FCSMs could be considered for use as root-end filling materials in endodontic microsurgery, particularly in complicated clinical situations which require rapid initial setting of the materials.


Assuntos
Compostos de Cálcio , Microcirurgia , Materiais Restauradores do Canal Radicular , Silicatos , Cálcio , Compostos de Cálcio/farmacologia , Feminino , Humanos , Masculino , Estudos Retrospectivos , Tratamento do Canal Radicular , Silicatos/farmacologia , Resultado do Tratamento
14.
J Endod ; 45(10): 1192-1199, 2019 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-31439355

RESUMO

INTRODUCTION: The purpose of the current population-based study was to analyze the 5-year survival rate of teeth that received nonsurgical endodontic treatment. Specifically, the variables affecting the 5-year survival rates of endodontically treated teeth were analyzed. METHODS: The present study included all endodontically treated teeth reported in 2010 in the National Health Insurance Cohort database of the Republic of Korea. By tracing the dental records of the sample to the end of 2015, the 5-year survival rates of the initial root canal treatment (RCT) and re-RCT were analyzed. Gender, age, institution type, diagnosis, arch type, tooth type, number of visits, and rubber dam usage were included in the analyses as confounding variables. RESULTS: In total, 2,866,749 teeth received initial RCT, and 173,429 teeth received re-RCT. Five-year survival rates were 90.85% and 88.42%, respectively. The survival rate of teeth that received initial RCT was significantly higher than that of those that received re-RCT. Specifically, re-RCT showed a significantly higher rate of extraction within 12 months than during other intervals. Furthermore, the following characteristics significantly positively affected the 5-year tooth survival rate: being female, <20 years of age, hospital as the institution type, mandibular teeth, anterior teeth, initial RCT, and use of rubber dam. CONCLUSIONS: Because of its high 5-year survival rate, endodontic treatment is an effective method for maintaining natural teeth.


Assuntos
Tratamento do Canal Radicular , Adolescente , Adulto , Estudos de Coortes , Feminino , Humanos , Masculino , República da Coreia , Estudos Retrospectivos , Resultado do Tratamento , Adulto Jovem
15.
J Clin Med ; 8(8)2019 Aug 16.
Artigo em Inglês | MEDLINE | ID: mdl-31426363

RESUMO

Trauma or injury to the dental pulp causes inflammation. This study compared the proteome of healthy pulp with inflamed pulp and traumatic pulp to identify the differentially expressed proteins in the diseased state. Five participants were grouped based on the pulpal status of the teeth: healthy, inflamed, or traumatic pulp. Pulp was extirpated and stored immediately in liquid nitrogen. Pulp tissues were subjected to 2-dimensional gel electrophoresis, and spot selection was performed. The selected spots were analyzed using liquid chromatography-tandem mass spectrometry and identified by correlating mass spectra to the proteomic databases. Fifteen spots showed increased expression in the inflamed and traumatic pulp. Annexin V, type II keratin, and hemoglobin levels were increased two-fold in the inflamed and traumatic pulp group and annexin V, mutant beta-actin, and hemoglobin were increased by ten-fold in the inflamed or traumatic pulp group, compared to levels in the healthy pulp group. Annexin V constituted two out of fifteen protein spots, and seemed to play a critical role in inhibiting inflammation and promoting the immune reaction. Further studies on this protein concerning its role in pulp repair are necessary to elucidate the underlying mechanisms.

16.
Materials (Basel) ; 12(15)2019 Jul 29.
Artigo em Inglês | MEDLINE | ID: mdl-31362338

RESUMO

The aim of this study was to assess the effect of three calcium silicate-based sealers (EndoSeal MTA, Nano-ceramic Sealer, and Wellroot ST) and two epoxy resin-based sealers (AH-Plus, AD Seal) on various aspects, such as cell viability, inflammatory response, and osteogenic potential, of human periodontal ligament stem cells (hPDLSCs). AH-Plus showed the lowest cell viability on hPDLSCs in all time periods in fresh media. In set media, hPDLSCs showed no significant differences in cell viability among all the tested materials. Wellroot ST showed the highest level of cell adhesion and the morphology of attached cells. AH-plus presented a significantly higher expression of IL-6 and IL-8 than the other sealers. AD Seal and three calcium silicate sealers showed high expression of the mesenchymal stem cell markers. ALP mRNA expression showed a significant increase in time-dependent manner on all of three calcium silicate-based sealers, which do not seem to interfere with the differentiation of hPDLSCs into osteoblasts. Based on the results from this study, calcium silicate-based sealers appear to be more biocompatible and less cytotoxic than epoxy resin-based sealers. Meanwhile, further and long-term clinical follow-up studies are required.

17.
J Clin Med ; 8(7)2019 Jul 07.
Artigo em Inglês | MEDLINE | ID: mdl-31284630

RESUMO

This study aimed to assess the efficacy of canal filling material removal using three different techniques after filling with a Gutta-Percha (GP) cone and calcium silicate-based sealer, by measuring the percentage of volume debris of GP and sealer remaining intracanal with micro computed tomography (micro-CT). The filling material was removed from 30 plastic teeth by a nickel-titanium (Ni-Ti) rotary retreatment system. Final irrigation was performed with 2 mL of saline and 10 specimens were randomly allocated to a conventional group. In the passive ultrasonic irrigation (PUI) group, ultrasonic irrigation was added to the conventional group (n = 10). In the Gentlefile Brush (GF Brush) group, irrigation with GF Brush was added to the conventional group (n = 10). Remaining filling material was measured using micro-CT imaging analysis. The total mean volume of residual filling material after retreatment in the conventional group, PUI group and GF Brush group were 4.84896 mm3, 0.80702 mm3, and 0.05248 mm3, respectively. The percentage of filling material remaining intracanal was 6.76% in the conventional group, 1.12% in the PUI group and 0.07% in the GF Brush group. This study shows that the cleaning effect of the GF Brush system is superior to those of Ni-Ti retreatment files and the PUI system in the apical area.

18.
Restor Dent Endod ; 44(2): e20, 2019 May.
Artigo em Inglês | MEDLINE | ID: mdl-31149618

RESUMO

Objectives: To achieve pulp-dentin complex regeneration with tissue engineering, treatment efficacies and safeties should be evaluated using in vivo orthotopic transplantation in a sufficient number of animals. Mice have been a species of choice in which to study stem cell biology in mammals. However, most pulp-dentin complex regeneration studies have used large animals because the mouse tooth is too small. The purpose of this study was to demonstrate the utility of the mouse tooth as a transplantation model for pulp-dentin complex regeneration research. Materials and Methods: Experiments were performed using 7-week-old male Institute of Cancer Research (ICR) mice; a total of 35 mice had their pulp exposed, and 5 mice each were sacrificed at 1, 2, 4, 7, 9, 12 and 14 days after pulp exposure. After decalcification in 5% ethylenediaminetetraacetic acid, the samples were embedded and cut with a microtome and then stained with hematoxylin and eosin. Slides were observed under a high-magnification light microscope. Results: Until 1 week postoperatively, the tissue below the pulp chamber orifice appeared normal. The remaining coronal portion of the pulp tissue was inflammatory and necrotic. After 1 week postoperatively, inflammation and necrosis were apparent in the root canals inferior to the orifices. The specimens obtained after experimental day 14 showed necrosis of all tissue in the root canals. Conclusions: This study could provide opportunities for researchers performing in vivo orthotopic transplantation experiments with mice.

19.
J Dent ; 86: 27-32, 2019 07.
Artigo em Inglês | MEDLINE | ID: mdl-31121242

RESUMO

OBJECTIVES: Conventional methods for detecting root fractures cannot assess their depth or bacterial contamination. This study was designed to measure the autofluorescence emitted from a root fracture, with the aim of determining whether this is a suitable method for quantifying the depth and bacterial invasion of a fracture. METHODS: This in vitro study investigated 33 mandibular second molars with periapical lesions that had been extracted after finding root fractures in endodontically treated teeth during intentional replantation or diagnostic surgery. The root fractures were scanned using a fluorescence technique, and the association between fluorescence parameters and fracture depth was analyzed. The significance of the association between the red fluorescence among autofluorescence parameters and bacterial contamination within the fracture was examined. RESULTS: When the depth of the root fractures was evaluated by micro computed tomography, the scattering of light in the fractures increased with depth, and there was a gradual increase in the quantitative fluorescence parameter indicating the deepest point (ΔFmax) in the fractures. In addition, we observed red fluorescence on the outer surface of deeper fractures. The tooth fractures exhibiting red fluorescence were evaluated for bacterial contamination associated with red-fluorescent porphyrin, which revealed bacterial invasion into these fractures. On the other hand, non-red-fluorescing fractures contained necrotic tissue, debris, and irritants. CONCLUSIONS: This viable fluorescent technique can potentially quantify the depth of root fractures and be used as a risk indicator for root fractures with periodontal inflammation. CLINICAL SIGNIFICANCE: The auto-fluorescence technique can be used to detect depth and bacterial contamination of root fractures. It is postulated that the auto-fluorescence can be used as a risk indicator of deep fractures and can replace conventional fracture detection methods.


Assuntos
Fraturas dos Dentes/microbiologia , Raiz Dentária/microbiologia , Dente não Vital , Humanos , Dente Molar , Microtomografia por Raio-X
20.
Materials (Basel) ; 12(8)2019 Apr 17.
Artigo em Inglês | MEDLINE | ID: mdl-30999582

RESUMO

This study aimed to analyze the effects of different calcium silicate cements (CSCs) on the inflammatory response and odontogenic differentiation of lipopolysaccharide-stimulated human dental pulp stem cells. Human dental pulp stem cells (hDPSCs) were stimulated with lipopolysaccharide (LPS) to induce inflammation. These LPS-induced dental pulp stem cells (LDPSCs) were cultured with ProRoot MTA, Biodentine, Retro MTA, and Dycal. Cell viability was evaluated using the Cell Counting Kit-8 assay. Interleukin (IL)-6, IL-8, and transforming growth factor (TGF)-ß1 cytokine levels were assessed using the enzyme-linked immunosorbent assay. The expressions of alkaline phosphatase (ALP), osteocalcin, and runt-related transcription factor 2 (RUNX2) were analyzed through real-time polymerase chain reaction. ProRoot MTA, Biodentine, and Retro MTA did not significantly decrease the cell viability of LDPSCs for up to 48 h (p < 0.05). Retro MTA significantly decreased the expression of IL-6 and IL-8 by LDPSCs. ProRoot MTA and Biodentine significantly reduced TGF-ß expression by LDPSCs (p < 0.05). Regarding odontogenic differentiation, all CSCs had no effect on ALP expression but increased the production of RUNX2 at 12 h.

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