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1.
Nutrients ; 12(9)2020 Aug 23.
Artigo em Inglês | MEDLINE | ID: mdl-32842462

RESUMO

Obesity is a notable risk factor for developing type 2 diabetes, augmenting the concern of obese diabetes (ObD). Anti-obesity and antioxidant effects of red pepper seeds extract (RPSE) have increased our expectations that RPSE would also improve the pathological phenotypes of obese diabetes. Therefore, we hypothesized that RPSE would have an anti-diabetic effect in ObD mice. Animals were assigned either as follows: (1) db/+, (2) db/db control, (3) RPSE (200 mg/kg bw), or (4) a comparative control (metformin 150 mg/kg bw). RPSE was orally administered daily for 8 weeks. As a result, RPSE supplementation improved diabetic phenotypes, including fasting glucose, hemoglobin (HbA1c), and insulin levels. Pro-inflammatory cytokines, tumor necrosis factor-alpha (TNF-α) and interleukin 6 (IL-6), and triglycerides were reduced in RPSE-treated mice. RPSE supplementation also diminished the rate-limiting enzymes of gluconeogenesis, including glucose 6-phosphatas (G6Pase) and phosphoenolpyruvate carboxykinase (PEPCK), in the liver. RPSE supplementation increased the phosphorylation of forkhead box protein O1 (FOXO1) and AMP-activated protein kinase (AMPK), which underlined the mechanism of the anti-diabetic effects of RPSE. Taken together, RPSE has the potential to improve glycemic control by repressing hepatic gluconeogenesis via the phosphorylation of FOXO1 and AMPK in ObD mice.

2.
J Transl Med ; 18(1): 236, 2020 06 12.
Artigo em Inglês | MEDLINE | ID: mdl-32532292

RESUMO

BACKGROUND: The in vitro production of mature human red blood cells (RBCs) from induced pluripotent stem cells (iPSCs) has been the focus of research to meet the high demand for blood transfusions. However, limitations like high costs and technological requirements restrict the use of RBCs produced by iPSC differentiation to specific circumstances, such as for patients with rare blood types or alloimmunized patients. In this study, we developed a detailed protocol for the generation of iPSC lines derived from peripheral blood of donors with O D-positive blood and rare blood types (D-and Jr(a-)) and subsequent erythroid differentiation. METHODS: Mononuclear cells separated from the peripheral blood of O D-positive and rare blood type donors were cultured to produce and expand erythroid progenitors and reprogrammed into iPSCs. A 31-day serum-free, xeno-free erythroid differentiation protocol was used to generate reticulocytes. The stability of iPSC lines was confirmed with chromosomal analysis and RT-PCR. Morphology and cell counts were determined by microscopy observations and flow cytometry. RESULTS: Cells from all donors were successfully used to generate iPSC lines, which were differentiated into erythroid precursors without any apparent chromosomal mutations. This differentiation protocol resulted in moderate erythrocyte yield per iPSC. CONCLUSIONS: It has previously only been hypothesized that erythroid differentiation from iPSCs could be used to produce RBCs for transfusion to patients with rare blood types or who have been alloimmunized. Our results demonstrate the feasibility of producing autologous iPSC-differentiated RBCs for clinical transfusions in patients without alternative options.

3.
FASEB J ; 2020 Jun 16.
Artigo em Inglês | MEDLINE | ID: mdl-32543734

RESUMO

Peroxidasin (PXDN) has been reported to crosslink the C-terminal non-collagenous domains of collagen IV (Col IV) by forming covalent sulfilimine bond. Here, we explored the physiological role of PXDN and its mechanism of action in endothelial cell survival and growth. Silencing of PXDN using siRNAs decreased cell proliferation without increase of the number of detached cells and decreased cell viability under serum-starved condition with increased fragmented nuclei and caspase 3/7 activity. Conditioned medium (CM) containing wild-type PXDN restored the proliferation of PXDN-depleted cells, but CM containing mutant PXDN with deletion of either N-terminal extracellular matrix (ECM) motifs or peroxidase domain failed to restore PXDN function. Accordingly, anti-PXDN antibody [raised against IgC2 (3-4) subdomain within ECM motifs] and peroxidase inhibitor phloroglucinol prevented the rescue of the PXDN-depleted cells by PXDN-containing CM. PXDN depletion resulted in loss of sulfilimine crosslinks, and decreased dense fibrillar network assembly of not only Col IV, but also fibronectin and laminin like in Col IV knockdown. Exogenous PXDN-containing CM restored ECM assembly as well as proliferation of PXDN-depleted cells. Accordingly, purified recombinant PXDN protein restored the proliferation and ECM assembly, and prevented cell death of the PXDN-depleted cells. PXDN depletion also showed reduced growth factors-induced phosphorylation of FAK and ERK1/2. In addition, siPXDN-transfected cell-derived matrix failed to provide full ECM-mediated activation of FAK and ERK1/2. These results indicate that both the ECM motifs and peroxidase activity are essential for the cellular function of PXDN and that PXDN is crucial for ECM assembly for survival and growth signaling.

4.
J Hazard Mater ; 399: 122949, 2020 Nov 15.
Artigo em Inglês | MEDLINE | ID: mdl-32502856

RESUMO

Designing nanostructured silicon, such as in the form of nanoparticles, wires, and porous structures, for high-performance Li-ion electrodes, has progressed significantly. These approaches have largely overcome the capacity fading of silicon electrodes from volume expansion during lithiation/de-lithiation. However, they involve high costs, complex processes, and hazardous precursors. Herein, we propose an electrochemical fabrication of silicon nanowires from waste rice husks via a molten salt process based on electrodeoxidation. The addition of NiO as an electric conductor improved the production efficiency and created pores in the nanowires after washing. The electrically produced high-purity silicon yielded high capacity, and the nanowires provided sufficient free volume to accommodate silicon electrode expansion, resulting in improved cycle life. The converted silicon nanowires from the molten salt process will help develop sustainable energy storage materials.

5.
Cardiovasc Intervent Radiol ; 43(7): 971-980, 2020 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-32385611

RESUMO

PURPOSE: Loss of fixation and seal represent a key problem when undertaking endovascular repair of abdominal aortic aneurysms (AAA) with hyperangulated necks (HAN). This study assesses the outcomes following the use of adjunct endostapling to supplement proximal aorto-prosthetic fixation in patients who have AAAs with HAN. METHODS: A retrospective review of a prospective database of 42 patients with HAN (> 60°) who underwent endovascular aneurysm repair (EVAR) with supplementary endostapling was undertaken. Primary outcomes assessed were: change in post-EVAR neck angulation at first post-procedure scan, freedom from type 1 endoleaks, migration and reintervention for proximal seal complications. Secondary parameters included assessment for neck dilatation, sac size changes and EndoAnchor distribution patterns. RESULTS: In total, 42 patients underwent EVAR between 2013 and 2019. There was one 30-day mortality resulting in 41 patients (34 male, 7 females aged 76.8 ± 8.9 years)) being analysed; 251 EndoAnchors were deployed in total, averaging 6 ± 2 per patient; 38 such cases were primary deployments. Neck angulation was 76.9 ± 14 degrees pre-EVAR and 50.2 ± 14.5 degrees post-procedure (p < .001, paired T test). Mean follow-up time was 18.5 (95% CI 13.3-23.9) months. One patient had persistent type Ia endoleak, successfully banded. There was 6.8 ± 10.2 mm sac size reduction (p < .001, paired T test). There were no other neck-related reinterventions, despite continued neck dilatation (3.2 ± 3.7 mm, p < .001, paired T test). CONCLUSION: This study suggests successful EVAR with adjunct endostapling for AAA with hyperangulated necks, with significant sac shrinkage and low rates of endoleaks, migration and reinterventions. More data are needed to consider influencing current instructions for use.

6.
Angew Chem Int Ed Engl ; 59(29): 11830-11835, 2020 Jul 13.
Artigo em Inglês | MEDLINE | ID: mdl-32304345

RESUMO

High-entropy alloys and compounds are becoming an important class of new materials due to their outstanding refractory and high-temperature properties. However, preparation in bulk quantities and in powder form via classical metallurgical methods is challenging. Here, we report the first synthesis of an ultra-high-temperature high-entropy carbide, (TiNbTaZrHf)C, via a facile electrochemical process. In this, a mixture of the individual metal oxides and graphite is deoxidised in a melt of CaCl2 at a temperature of only 1173 K. The (TiNbTaZrHf)C prepared is single-phase fcc and has a powdery morphology with a particle-size range of 15-80 nm. Such materials are in demand for modern additive manufacturing techniques, while preliminary tests have also indicated a possible application in supercapacitors. The successful synthesis of (TiNbTaZrHf)C powder may now guide the way towards establishing the electrochemical route for the preparation of many other entropy-stabilised materials.

7.
Nature ; 580(7803): 376-380, 2020 04.
Artigo em Inglês | MEDLINE | ID: mdl-32296182

RESUMO

Mechanosensory feedback from the digestive tract to the brain is critical for limiting excessive food and water intake, but the underlying gut-brain communication pathways and mechanisms remain poorly understood1-12. Here we show that, in mice, neurons in the parabrachial nucleus that express the prodynorphin gene (hereafter, PBPdyn neurons) monitor the intake of both fluids and solids, using mechanosensory signals that arise from the upper digestive tract. Most individual PBPdyn neurons are activated by ingestion as well as the stimulation of the mouth and stomach, which indicates the representation of integrated sensory signals across distinct parts of the digestive tract. PBPdyn neurons are anatomically connected to the digestive periphery via cranial and spinal pathways; we show that, among these pathways, the vagus nerve conveys stomach-distension signals to PBPdyn neurons. Upon receipt of these signals, these neurons produce aversive and sustained appetite-suppressing signals, which discourages the initiation of feeding and drinking (fully recapitulating the symptoms of gastric distension) in part via signalling to the paraventricular hypothalamus. By contrast, inhibiting the same population of PBPdyn neurons induces overconsumption only if a drive for ingestion exists, which confirms that these neurons mediate negative feedback signalling. Our findings reveal a neural mechanism that underlies the mechanosensory monitoring of ingestion and negative feedback control of intake behaviours upon distension of the digestive tract.


Assuntos
Ingestão de Alimentos , Retroalimentação , Neurônios/fisiologia , Animais , Encefalinas/genética , Encefalinas/metabolismo , Masculino , Camundongos , Camundongos Endogâmicos C57BL , Precursores de Proteínas/genética , Precursores de Proteínas/metabolismo , Trato Gastrointestinal Superior/fisiologia
8.
BMC Health Serv Res ; 20(1): 166, 2020 Mar 04.
Artigo em Inglês | MEDLINE | ID: mdl-32131818

RESUMO

BACKGROUND: The look-back period is needed to define baseline population for estimating incidence. However, short look-back period is known to overestimate incidence of diseases misclassifying prevalent cases to incident cases. The purpose of this study is to evaluate the impact of the various length of look-back period on the observed incidences of uterine leiomyoma, endometriosis and adenomyosis, and to estimate true incidences considering the misclassification errors in the longitudinal administrative data in Korea. METHODS: A total of 319,608 women between 15 to 54 years of age in 2002 were selected from Korea National Health Insurance Services (KNHIS) cohort database. In order to minimize misclassification bias incurred when applying various length of look-back period, we used 11 years of claim data to estimate the incidence by equally setting the look-back period to 11 years for each year using prediction model. The association between the year of diagnosis and the number of prevalent cases with the misclassification rates by each look-back period was investigated. Based on the findings, prediction models on the proportion of misclassified incident cases were developed using multiple linear regression. RESULTS: The proportion of misclassified incident cases of uterine leiomyoma, endometriosis and adenomyosis were 32.8, 10.4 and 13.6% respectively for the one-year look-back period in 2003. These numbers decreased to 6.3% in uterine leiomyoma and - 0.8% in both endometriosis and adenomyosis using all available look-back periods (11 years) in 2013. CONCLUSION: This study demonstrates approaches for estimating incidences considering the different proportion of misclassified cases for various length of look-back period. Although the prediction model used for estimation showed strong R-squared values, follow-up studies are required for validation of the study results.

9.
J Korean Med Sci ; 35(4): e17, 2020 Feb 03.
Artigo em Inglês | MEDLINE | ID: mdl-31997613

RESUMO

BACKGROUND: Severe and life-threatening drug eruptions include drug reaction with eosinophilia and systemic symptoms (DRESS), Stevens-Johnson syndrome (SJS), and toxic epidermal necrolysis (TEN). One class of medications that has been highly associated with such drug eruptions is antiepileptic drugs (AEDs). We attempt to investigate drug eruptions associated with AEDs as a class, as well as with individual AEDs, in Korea. METHODS: We used the Korea Institute of Drug Safety and Risk Management - Korea Adverse Event Reporting System (KIDS-KAERS) database, a nationwide database of adverse events reports, between January 2008 and December 2017 to investigate the reporting count of all drug eruptions and calculated the ratio of DRESS/SJS/TEN reports for each AED. RESULTS: Among a total of 2,942 reports, most were of rash/urticaria (2,702, 91.8%), followed by those of DRESS (109, 3.7%), SJS (106, 3.6%), and TEN (25, 0.85%). The common causative AEDs were lamotrigine (699, 23.8%), valproic acid (677, 23%), carbamazepine (512, 17.4%), oxcarbazepine (320, 10.9%), levetiracetam (181, 6.2%), and phenytoin (158, 5.4%). In limited to severe drug eruptions (DRESS, SJS, and TEN; total 241 reports), the causative AEDs were carbamazepine (117, 48.8%), lamotrigine (57, 23.8%), valproic acid (20, 8.3%), phenytoin (15, 6.3%), and oxcarbazepine (10, 4.2%). When comparing aromatic AED with non-aromatic AED, aromatic AEDs were more likely to be associated with severe drug eruption (aromatic AEDs: 204/1,793 versus non-aromatic AEDs: 37/1,149; OR, 3.86; 95% CI, 2.7-5.5). Death was reported in 7 cases; DRESS was the most commonly reported adverse event (n = 5), and lamotrigine was the most common causative AED (n = 5). CONCLUSION: Although most cutaneous drug eruptions in this study were rash or urticaria, approximately 8% of reports were of severe or life-threatening adverse drug reactions, such as SJS, TEN, or DRESS. When hypersensitivity skin reactions occurred, aromatic AEDs were associated with 4 fold the risk of SJS/TEN/DRESS compared with non-aromatic AEDs. Our findings further emphasize that high risk AEDs should be prescribed under careful monitoring, and early detection and prompt interventions are needed to prevent severe complications.


Assuntos
Sistemas de Notificação de Reações Adversas a Medicamentos , Anticonvulsivantes , Erupção por Droga/epidemiologia , Adolescente , Adulto , Sistemas de Notificação de Reações Adversas a Medicamentos/estatística & dados numéricos , Idoso , Anticonvulsivantes/efeitos adversos , Criança , Pré-Escolar , Bases de Dados Factuais , Síndrome de Hipersensibilidade a Medicamentos/epidemiologia , Feminino , Humanos , Lactente , Masculino , Pessoa de Meia-Idade , República da Coreia , Fatores de Risco , Síndrome de Stevens-Johnson/epidemiologia , Adulto Jovem
11.
Int J Mol Sci ; 20(24)2019 Dec 05.
Artigo em Inglês | MEDLINE | ID: mdl-31817535

RESUMO

Peroxidasin (PXDN) is a unique peroxidase containing extracellular matrix motifs and stabilizes collagen IV networks by forming sulfilimine crosslinks. PXDN gene knockout in Caenorhabditis elegans (C. elegans) and Drosophila results in the demise at the embryonic and larval stages. PXDN mutations lead to severe eye disorders, including microphthalmia, cataract, glaucoma, and anterior segment dysgenesis in humans and mice. To investigate how PXDN loss of function affects organ development, we generated Pxdn knockout mice by deletion of exon 1 and its 5' upstream sequences of the Pxdn gene using the CRISPR/Cas9 system. Loss of both PXDN expression and collagen IV sulfilimine cross-links was detected only in the homozygous mice, which showed completely or almost closed eyelids with small eyes, having no apparent external morphological defects in other organs. In histological analysis of eye tissues, the homozygous mice had extreme defects in eye development, including no eyeballs or drastically disorganized eye structures, whereas the heterozygous mice showed normal eye structure. Visual function tests also revealed no obvious functional abnormalities in the eyes between heterozygous mice and wild-type mice. Thus, these results suggest that PXDN activity is essential in eye development, and also indicate that a single allele of Pxdn gene is sufficient for eye-structure formation and normal visual function.


Assuntos
Anoftalmia , Olho/crescimento & desenvolvimento , Deleção de Genes , Peroxidases/deficiência , Animais , Anoftalmia/genética , Anoftalmia/metabolismo , Anoftalmia/patologia , Sistemas CRISPR-Cas , Colágeno Tipo IV/genética , Colágeno Tipo IV/metabolismo , Olho/patologia , Camundongos , Camundongos Knockout , Peroxidases/metabolismo , Visão Ocular/genética
12.
Artigo em Inglês | MEDLINE | ID: mdl-31426112

RESUMO

BACKGROUND: In diabetic retinopathy (DR), neutrophil extracellular traps (NET) and kallikrein-kinin system are considered as contributing factors. However, the detail activation mechanisms has not been fully understood. Since the NET could provide negative-charged surface for factor XII activation and the activated factor XII (XIIa) can initiate kallikrein-kinin system, this study investigated whether patients with DR show activation of NET, factor XII and kallikrein-kinin system. METHODS: The markers related to NET (DNA-histone complex) and kallikrein-kinin system (high-molecular-weight kininogen, prekallikrein, bradykinin) and factor XIIa were measured in 253 patients with diabetes. To access ex vivo effect of glucose, DNA-histone complex and factor XIIa were measured in whole blood stimulated by glucose. RESULTS: The circulating level of DNA-histone complex and factor XIIa were significantly higher in patients with DR than those without DR. In logistic regression analysis, DNA-histone complex, factor XIIa, and high-molecular-weight kininogen were the risk factors of DR. In recursive partitioning analysis, among patients with diabetes duration less than 10 years, patients with high level of DNA-histone complex (>426 AU) showed high risk of DR. In ex vivo experiment, glucose significantly elevated both DNA-histone complex and factor XIIa. CONCLUSION: Our findings suggest that activation of factor XII and kallikrein-kinin system combined with NET formation actively occur in patients with DR and circulating levels of DNA-histone complex, factor XIIa and HMWK can be potential biomarkers to estimate the risk of DR. Strategies against factor XII activation may be beneficial to inhibit DR.

13.
Comput Methods Biomech Biomed Engin ; 22(14): 1153-1161, 2019 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-31339367

RESUMO

A 3D anatomically-based finite-element foot model was adopted for predicting von Mises stresses within tibiotalar cartilage following 5 km barefoot running. To compare this predicted stress with T2 maps, magnetic resonance scans of the right ankle and plantar pressure were obtained from ten novices and ten marathon-experienced runners before and after running. Following running, tibiotalar cartilage stress was decreased in experienced runners. This corresponded with T2 values that did not change between pre- and post-running suggesting no increase in cartilage fluid levels. In contrast, novices maintained the same level of von Mises stress and this corresponded with a significant T2 increase in tibiotalar cartilage.


Assuntos
Atletas , Cartilagem/fisiopatologia , Pé/fisiopatologia , Corrida/fisiologia , Estresse Mecânico , Tálus/fisiopatologia , Adulto , Cartilagem/diagnóstico por imagem , Feminino , Pé/diagnóstico por imagem , Humanos , Imagem por Ressonância Magnética , Masculino , Pressão , Tálus/diagnóstico por imagem
14.
J Econ Entomol ; 112(4): 1611-1617, 2019 08 03.
Artigo em Inglês | MEDLINE | ID: mdl-31329900

RESUMO

Whitefly pests, including the sweetpotato whitefly, Bemisia tabaci (Gennadius), and the greenhouse whitefly, Trialeurodes vaporariorum (Westwood), are economically important in agriculture. With the annual growth of the domestic fresh fruit export market, various quarantine treatment methods are being used to export strawberries of better quality. The objective of the present study was to evaluate the effects of gamma rays on the development and reproductive sterility of B. tabaci and T. vaporariorum. In both species, the eggs were completely inhibited from hatching at 50 Gy, and the emergence of third-instar nymphs was completely suppressed at 150 Gy. Some adult B. tabaci and T. vaporariorum spawning occurred at 100 and 70 Gy, respectively; however, at these irradiation levels, F1 hatchability was completely inhibited. Dosimetry results showed that the penetrating power of gamma ray in the strawberry-filled box was the lowest at the mid-box position. Therefore, B. tabaci and T. vaporariorum were placed in the middle of the strawberry-filled box and irradiated. A gamma-ray irradiation of 100 Gy suppressed the development and reproduction of eggs and adults in both B. tabaci and T. vaporariorum. Our data suggest that at least 100 Gy should be used for the control of these two species of whitefly for strawberry export.


Assuntos
Fragaria , Hemípteros , Animais , Ninfa
15.
Exp Appl Acarol ; 78(1): 65-78, 2019 May.
Artigo em Inglês | MEDLINE | ID: mdl-31069572

RESUMO

The acaricidal activity of 30 essential oils against the poultry red mite, Dermanyssus gallinae, female adults and behavioral responses of the mites to these essential oils were investigated. Cinnamon bark oil and clove bud oil showed 100% acaricidal activity after 24 h in the 1.3 µg/m2 treatment. In addition, four components in cinnamon bark oil and three components in clove bud oil were identified using gas chromatography-mass spectrometry. Cinnamon bark oil showed the highest LD50 value among all of the components, and eugenol showed 0.97-fold higher relative toxicity (RT) than the other components of clove bud oil. The fumigant effects of both essential oils and their seven components were observed using a vapor phase toxicity bioassay. All the substances showed repellent activity except for cinnamyl acetate, which did not show any repellent response even in the > 10 µg treatment. In the experiment using the T-tube olfactometer with the 10 µg treatment of each substance, D. gallinae female adults responded to all the substances except cinnamyl acetate. However, eugenol and eugenol acetate showed an attractant effect after 240 and 120 min of treatment, respectively. These results suggest that the two studied essential oils and their components may be used as control agents against D. gallinae.


Assuntos
Acaricidas , Óleo de Cravo , Ácaros , Óleos Voláteis , Controle de Ácaros e Carrapatos , Animais , Feminino
16.
J Econ Entomol ; 112(5): 2149-2156, 2019 09 23.
Artigo em Inglês | MEDLINE | ID: mdl-31115458

RESUMO

The fumigation activity of phosphine (PH3) and ethyl formate (EF) and their phytotoxicity to 13 imported foliage nursery plant species were evaluated. The lethal concentration and time (LCT99) values of the PH3 indicated that the susceptibility of the nymphs (3.95 and <0.45 mg·h/liter, respectively) was higher than that of the adults (5.29 and 3.66 mg·h/liter, respectively) of two mealybugs [Pseudococcus longispinus (Targioni-Tozzetti) and P. orchidicola Takahashi]. The highest concentration reduction rate of PH3 and EF on the 13 foliage nursery plants in the 12-liter desiccator was 41.5% for Heteropanax fragrans and 71.7% for Schefflera arboricola, respectively, which indicates that PH3 has a lower sorption rate than EF. The phytotoxicities of PH3-treated foliage nursery plants did not significantly differ from those of the nontreated plants, but EF caused phytotoxicity in 11 foliage nursery plants a week after treatment. When the exposure time of PH3 increased to 24 h, the adults and nymphs of both mealybug species showed 100% mortality in the 0.5 m3 fumigation chamber. In the 10 m3 fumigation container used in the field, there was 100% mortality of both mealybugs after treatment with 2 g/m3 PH3 for 24 h at 16°C. These results indicate that EF is not a suitable mealybug fumigant due to its high sorption and phytotoxicity to foliage nursery plants, despite fumigation activity against the two species. However, PH3 seems to be suitable for mealybug fumigation in foliage nursery plants and can be used as a substitute for methyl bromide.


Assuntos
Hemípteros , Fosfinas , Animais , Ésteres do Ácido Fórmico , Fumigação
17.
Artigo em Inglês | MEDLINE | ID: mdl-30947593

RESUMO

Amine-functionalized magnetite (nFe3O4-NH2) of two different sizes, 10 nm and 250 nm, were compared as environmental adsorbents. They were synthesized by co-precipitation (10 nm-nFe3O4-NH2) and solvothermal (250 nm-nFe3O4-NH2) methods, respectively. The prepared amine-functionalized magnetite was characterized by scanning electron microscope, transmission electron microscope, X-ray diffraction, vibrating sample magnetometer, Fourier transform infrared spectroscopy, size distribution analysis and surface area analysis to compare the properties of different sizes of nFe3O4-NH2. Both nFe3O4-NH2 contained cubic Fe3O4 crystalline structure. The 250 nm-nFe3O4-NH2 exhibited higher magnetic saturation value than the 10 nm-nFe3O4-NH2, but both could be separated from an aqueous solution using an external magnet. The surface area and pore volume of the smaller-sized 10 nm-nFe3O4-NH2 was larger than that of 250 nm-nFe3O4-NH2, but stronger aggregation was observed in the 10 nm-nFe3O4-NH2. Batch adsorption of lead indicated that the 10 nm-nFe3O4-NH2 was a better adsorbent than the 250 nm-nFe3O4-NH2. The maximum adsorption capacity of lead for the 10 nm-nFe3O4-NH2 and the 250 nm-nFe3O4-NH2 were 74.48 mg g-1 and 54.54 mg g-1, respectively. The stronger aggregation of nanoparticles with a smaller particle size did not affect the superior performance of the 10 nm-nFe3O4-NH2 as an environmental adsorbent.


Assuntos
Aminas/química , Chumbo/análise , Nanopartículas de Magnetita/química , Poluentes Químicos da Água/análise , Adsorção , Fenômenos Magnéticos , Modelos Teóricos , Tamanho da Partícula , Soluções , Propriedades de Superfície
18.
PLoS One ; 14(4): e0216055, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31034495

RESUMO

INTRODUCTION: Neutrophils can generate extracellular net-like structures by releasing their DNA-histone complexes and antimicrobial peptides, which is called neutrophil extracellular traps (NETs). Various stimuli can induce NET formation. In particular, neutrophils and NET formation are abundant in tumor tissue. This study investigated how cancer cells induce NET formation and whether this NET formation promotes plasma thrombin generation and cancer progression. METHODS: Induction of NET formation by a pancreatic cancer cell line (AsPC-1) was assessed by measuring the histone-DNA complex level. The endogenous thrombin potential (ETP) was measured by thrombin generation assay. In vitro migration, invasion, and tubule formation assays were performed. The circulating levels of NET markers and hypercoagulability markers were assessed in 62 patients with pancreatobiliary malignancy and 30 healthy controls. RESULTS: AsPC-1 significantly induced NET formation in a dose-dependent manner. Conditioned medium (CM) from AsPC-1 also induced NETs. Interestingly, NET-formation was abolished by heat-inactivated CM, but not by lipid-extracted CM, suggesting an important role of protein components. A reactive oxygen species inhibitor did not inhibit cancer cell-induced NET formation, but prostaglandin E1 (PGE1, cyclic adenosine monophosphate inducer) and antithrombin did. NETs significantly increased ETP of normal plasma. Of note, NETs promoted cancer cell migration and invasion as well as angiogenesis, which were inhibited by histone-binding agents (heparin, polysialic acid), a DNA-degrading enzyme, and Toll-like receptor neutralizing antibodies. In patients with pancreatobiliary malignancy, elevated NET markers correlated well with hypercoagulability makers. CONCLUSION: Our findings indicate that cancer cell-induced NET formation enhances both hypercoagulability and cancer progression and suggest that inhibitors of NET formation such as PGE1 and antithrombin can be potential therapeutics to reduce both hypercoagulability and cancer progression.


Assuntos
Progressão da Doença , Armadilhas Extracelulares/metabolismo , Neoplasias Pancreáticas/patologia , Trombofilia/patologia , Adulto , Biomarcadores Tumorais/metabolismo , Linhagem Celular Tumoral , Movimento Celular , AMP Cíclico/metabolismo , Células Endoteliais/patologia , Humanos , Invasividade Neoplásica , Neovascularização Patológica , Espécies Reativas de Oxigênio/metabolismo , Trombina/metabolismo
19.
Clin Lab ; 65(3)2019 Mar 01.
Artigo em Inglês | MEDLINE | ID: mdl-30868842

RESUMO

BACKGROUND: Increasing interest has focused on the development of new assays more specific for APS and application of multiple combinations of anti-phospholipid antibodies (aPLs). This study explored the thrombotic risk of non-criteria aPLs measured by a new line immunoassay (LIA) and the benefit of additional non-criteria aPLs results to the APS diagnosis. METHODS: LIA were performed to detect 9 aPLs in 180 patients requested for lupus anticoagulant (LA) measurement. Antibodies against anti-cardiolipin (CL), ß2 glycoprotein I (GPI), and ß2GPI domain I were measured by ELISA. RESULTS: The agreement percentages for IgG/IgM anti-CL and anti-ß2GPI between ELISA and LIA (anti-CL 68.2% and 82.6%; anti-ß2GPI 71.7% and 93.2%, respectively). Among 9 aPLs measured by LIA, single presence IgG of anti-phosphatidylserine (odds ratio (OR) 16.477) and anti-phosphatidic acid (OR 9.625) predicted higher thrombotic risk than anti-ß2GPI (OR 5.538). Other aPLs measured by LIA (anti-prothrombin, anti-annexin V, anti-phosphatidylinositol, phosphatidylethanolamine, and anti-phosphatidylglycerol) did not show any significant thrombotic risk. Addition of the 2 non-criteria aPLs (anti-phosphatidylserine and anti-phosphatidic acid) to the established APS criteria increased the diagnostic specificity and accuracy for thrombosis. The positive rates of anti-ß2GPI and anti-phosphatidylserine measured by LIA were quite high in patients with positive anti-ß2GPI domain I. CONCLUSIONS: The anti-phosphatidylserine and anti-phosphatidic acid among non-criteria aPLs have a high likeli-hood as new markers for thrombotic prediction.


Assuntos
Anticorpos Antifosfolipídeos/análise , Síndrome Antifosfolipídica/diagnóstico , Trombose/imunologia , Adulto , Feminino , Humanos , Imunoensaio , Masculino , Ácidos Fosfatídicos/imunologia , Fosfatidilserinas/imunologia , Valor Preditivo dos Testes , beta 2-Glicoproteína I/imunologia
20.
Nanomaterials (Basel) ; 9(3)2019 03 07.
Artigo em Inglês | MEDLINE | ID: mdl-30866508

RESUMO

In the published paper [¹], there was a typo error mistake in Equation (5), which was supposed to be expressed as " log Z t + n log t = log K T - m log Φ " instead of "log Zt + n log t = log KT - ml" [...].

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