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1.
Medicine (Baltimore) ; 100(31): e26844, 2021 Aug 06.
Artigo em Inglês | MEDLINE | ID: mdl-34397857

RESUMO

RATIONALE: Primary signet ring cell carcinoma of the uterine cervix is extremely rare and the clinical characteristics and prognosis are not well known and there are no specific guidelines for treatment. PATIENT CONCERNS: A 43-year-old woman was referred to our hospital for abnormal uterine bleeding lasting 1 month. DIAGNOSES: Histological examination revealed a signet ring cell carcinoma of the uterine cervix. After evaluation of extragenital origin, the patient was diagnosed International Federation of Gynecology and Obstetrics stage IIIC1 primary signet ring cell carcinoma or the uterine cervix. INTERVENTION: The patient was prescribed concomitant chemo-radiation followed by intracavitary brachytherapy. OUTCOMES: She showed no evidence of disease after treatment but, it recurred after 7 months of last treatment. LESSONS: Different approaches to diagnosis and treatment of this rare disease are needed and molecular pathological studies related to the onset of the disease are required.


Assuntos
Carcinoma de Células em Anel de Sinete , Colo do Útero , Quimiorradioterapia/métodos , Cisplatino/administração & dosagem , Neoplasias do Colo do Útero , Esfregaço Vaginal/métodos , Adulto , Antineoplásicos/administração & dosagem , Biópsia/métodos , Braquiterapia/métodos , Carcinoma de Células em Anel de Sinete/patologia , Carcinoma de Células em Anel de Sinete/fisiopatologia , Carcinoma de Células em Anel de Sinete/terapia , Colo do Útero/diagnóstico por imagem , Colo do Útero/patologia , Evolução Fatal , Feminino , Humanos , Recidiva Local de Neoplasia/patologia , Papillomaviridae/isolamento & purificação , Retratamento/métodos , Neoplasias do Colo do Útero/patologia , Neoplasias do Colo do Útero/fisiopatologia , Neoplasias do Colo do Útero/terapia , Hemorragia Uterina/diagnóstico , Hemorragia Uterina/etiologia
2.
Int J Med Sci ; 18(13): 2828-2834, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34220310

RESUMO

Objective: Predict the presence of lymphovascular space invasion (LVSI), using uterine factors such as tumor diameter (TD), grade, and depth of myometrial invasion (MMI). Develop a predictive model that could serve as a marker of LVSI in women with endometrial cancer (EC). Methods: Data from 888 patients with endometrioid EC who were treated between January 2009 and December 2018 were reviewed. The patients' data were retrieved from six institutions. We assessed the differences in the clinicopathological characteristics between patients with and without LVSI. We performed logistic regression analysis to determine which clinicopathological characteristics were the risk factors for positive LVSI status and to estimate the odds ratio (OR) for each covariate. Using the risk factors and OR identified through this process, we created a model that could predict LVSI and analyzed it further using receiver operating characteristic curve analysis. Results: In multivariate logistic regression analysis, tumor size (P = 0.027), percentage of MMI (P < 0.001), and presence of cervical stromal invasion (P = 0.002) were identified as the risk factors for LVSI. Based on the results of multivariate logistic regression analysis, we developed a simplified LVSI prediction model for clinical use. We defined the "LVSI index" as "TD×%MMI×tumor grade×cervical stromal involvement." The area under curve was 0.839 (95% CI= 0.809-0.869; sensitivity, 74.1%; specificity, 80.5%; negative predictive value, 47.3%; positive predictive value, 8.6%; P < 0.001), and the optimal cut-off value was 200. Conclusion: Using the modified risk index of LVSI, it is possible to predict the presence of LVSI in women with endometrioid endometrial cancer. Our prediction model may be an appropriate tool for integration into the clinical decision-making process when assessed either preoperatively or intraoperatively.

3.
J Environ Manage ; 288: 112415, 2021 Jun 15.
Artigo em Inglês | MEDLINE | ID: mdl-33774562

RESUMO

Understanding the dynamics of harmful algal blooms is important to protect the aquatic ecosystem in regulated rivers and secure human health. In this study, artificial neural network (ANN) and support vector machine (SVM) models were used to predict algae alert levels for the early warning of blooms in a freshwater reservoir. Intensive water-quality, hydrodynamic, and meteorological data were used to train and validate both ANN and SVM models. The Latin-hypercube one-factor-at-a-time (LH-OAT) method and a pattern search algorithm were applied to perform sensitivity analyses for the input variables and to optimize the parameters of the models, respectively. The results indicated that the two models well reproduced the algae alert level based on the time-lag input and output data. In particular, the ANN model showed a better performance than the SVM model, displaying a higher performance value in both training and validation steps. Furthermore, a sampling frequency of 6- and 7-day were determined as efficient early-warning intervals for the freshwater reservoir. Therefore, this study presents an effective early-warning prediction method for algae alert level, which can improve the eutrophication management schemes for freshwater reservoirs.


Assuntos
Ecossistema , Água Doce , Surtos de Doenças , Eutrofização , Proliferação Nociva de Algas , Humanos , Aprendizado de Máquina , Qualidade da Água
4.
PLoS One ; 16(2): e0245799, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-33606716

RESUMO

Peroxisomes are metabolically active organelles which are known to exert anti-inflammatory effects especially associated with the synthesis of mediators of inflammation resolution. However, the role of catalase and effects of peroxisome derived reactive oxygen species (ROS) caused by lipid peroxidation through 4-hydroxy-2-nonenal (4-HNE) on lipopolysaccharide (LPS) mediated inflammatory pathway are largely unknown. Here, we show that inhibition of catalase by 3-aminotriazole (3-AT) results in the generation of peroxisomal ROS, which contribute to leaky peroxisomes in RAW264.7 cells. Leaky peroxisomes cause the release of matrix proteins to the cytosol, which are degraded by ubiquitin proteasome system. Furthermore, 3-AT promotes the formation of 4HNE-IκBα adduct which directly interferes with LPS induced NF-κB activation. Even though, a selective degradation of peroxisome matrix proteins and formation of 4HNE- IκBα adduct are not directly related with each other, both of them are could be the consequences of lipid peroxidation occurring at the peroxisome membrane.


Assuntos
Catalase/antagonistas & inibidores , Inibidores Enzimáticos/farmacologia , Lipopolissacarídeos/farmacologia , Peroxissomos/efeitos dos fármacos , Peroxissomos/metabolismo , Animais , Citocinas/genética , Regulação da Expressão Gênica/efeitos dos fármacos , Inflamação/induzido quimicamente , Inflamação/metabolismo , Camundongos , NF-kappa B/metabolismo , Complexo de Endopeptidases do Proteassoma/metabolismo , Proteólise/efeitos dos fármacos , Células RAW 264.7 , RNA Mensageiro/genética , Espécies Reativas de Oxigênio/metabolismo
5.
Artigo em Inglês | MEDLINE | ID: mdl-32075719

RESUMO

Peroxisomes are metabolically active oxygen demanding organelles with a high abundance of oxidases making it vulnerable to low oxygen levels such as hypoxic conditions. However, the exact mechanism of peroxisome degradation in hypoxic condition remains elusive. In order to study the mechanism of peroxisome degradation in hypoxic condition, we use Dimethyloxaloylglycine (DMOG), a cell-permeable prolyl-4-hydroxylase inhibitor, which mimics hypoxic condition by stabilizing hypoxia-inducible factors. Here we report that DMOG degraded peroxisomes by selectively activating pexophagy in a HIF-2α dependent manner involving autophagy receptor p62. Furthermore, DMOG not only increased peroxisome turnover by pexophagy but also reduced HIF-2α dependent peroxisome proliferation at the transcriptional level. Taken together, our data suggest that hypoxic condition is a negative regulator for peroxisome abundance through increasing pexophagy and decreasing peroxisome proliferation in HIF-2α dependent manner.

6.
Water Res ; 154: 387-401, 2019 05 01.
Artigo em Inglês | MEDLINE | ID: mdl-30822599

RESUMO

We examined the relationship between downstream algal growth potential and the spatial environmental factors of both upland areas and stream buffer zones using spatial analysis and generalized additive models (GAMs). The models employed site-representative concentrations of chlorophyll a (Chl-a) from a total of 688 national water quality monitoring stations and the spatial factors of the corresponding 688 watersheds. The spatial environmental factors included topography, climate, land use class, soil type, and proximity of the monitoring station to the weir downstream and wastewater treatment plants (WWTPs). The explanatory power (adjusted R2 or Radj2) of the models was used to compare different spatial influential scales defined by stream buffers and upstream circular buffers. The spatial environmental factors of the entire watershed area better explained the inter-station variation in Chl-a than did those of the stream buffer and/or upstream circular buffer areas. However, the spatial environmental factors of watershed areas more than 25 km upstream circular buffer zones had only minor influence on the explainability of the models with regards to the inter-station variation in Chl-a levels. Generally, land use patterns were more strongly related to the inter-station Chl-a variation than were point sources of pollutants such as WWTPs. The two most influencing land uses on the inter-station Chl-a variation were urban and agricultural land uses, with varying relative contributions depending on the spatial influential scale: In general relative contribution of urban land use was larger at a larger spatial influential scale while that of agricultural land use showed an opposite trend. In addition, the proximity to the weir downstream explained high Chl-a concentrations in the stream water. Relative importance and causal effects of the spatial environmental variables to instream Chl-a were established based on this national scale correlative analysis, leading to decision-making with the goal of controlling instream algal growth.


Assuntos
Agricultura , Clorofila A , Clima , Monitoramento Ambiental , Solo , Análise Espacial
7.
Environ Sci Pollut Res Int ; 25(30): 30044-30055, 2018 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-30076551

RESUMO

A number of severe norovirus outbreaks due to the consumption of contaminated shellfish have been reported recently. In this study, we evaluated the distribution of coliphage densities to determine their efficacy as fecal indicators of enteric viruses, including noroviruses, in water samples collected from a shellfish growing area in Republic of Korea over a period of approximately 1 year. Male-specific and somatic coliphages in water samples were analyzed using the single agar layer method, and norovirus genogroups I and II, which infect mainly humans, were analyzed using duplex reverse transcription quantitative PCR. Male-specific and somatic coliphages were detected widely throughout the study area. Several environmental parameters, including salinity, precipitation, temperature, and wind speed were significantly correlated with coliphage concentrations (P < 0.05). Moreover, the concentrations of male-specific coliphages were positively correlated with the presence of human noroviruses (r = 0.443; P < 0.01). The geospatial analysis with coliphage concentrations using a geographic information system revealed that densely populated residential areas were the major source of fecal contamination. Our results indicate that coliphage monitoring in water could be a useful approach to prevent norovirus contamination in shellfish.


Assuntos
Colífagos/isolamento & purificação , Norovirus/isolamento & purificação , Frutos do Mar/virologia , Animais , Colífagos/classificação , Colífagos/genética , Monitoramento Ambiental , Fezes/virologia , Contaminação de Alimentos/análise , Sistemas de Informação Geográfica , Humanos , Norovirus/classificação , Norovirus/genética , República da Coreia , Microbiologia da Água
8.
Int J Med Sci ; 15(9): 915-920, 2018.
Artigo em Inglês | MEDLINE | ID: mdl-30008604

RESUMO

This study aimed to determine the role of asymptomatic bacterial sexually transmitted infections (STIs), such as Chlamydia trachomatis (Ct), Mycoplasma genitalium (Mg), Mycoplasma hominis (Mh), and Ureaplasma urealyticum (Uu) in human papillomavirus (HPV) infection. In total, 264 asymptomatic outpatients aged between 21 and 80 years were prospectively enrolled in this study during routine gynecological screening tests. Specimens collected with a Cervex Brush were routinely analyzed with the Hybrid Capture 2 assay for HPV. Simultaneously, a specimen obtained with an endocervical swab was used to detect Ct and Mg with a monoplex real-time polymerase chain reaction (PCR) and to confirm Mh and Uu with a Mycoplasma IST 2 kit. The detection rates (%) of HPV, Ct, Mg, Mh, and Uu were 82/264 (31.1), 6/264 (2.3), 5/264 (1.9), 16/264 (6.1), and 95/264 (36.0), respectively. Of 95 Uu, 32 (33.7%) showed high density colonization (HDC, ≥104 color-changing units/mL). HDC-Uu was significantly associated with HPV infection (p=0.014, chi-square test). Mg infection and Mh infection were not associated with HPV infection (p=0.981 and p=0.931, chi-square test). Age was not associated with HPV infection or bacterial infection. Our data suggested that asymptomatic HDC-Uu was closely associated with HPV infection. Therefore, simultaneous evaluation for Uu and HPV should be performed during gynecological screening, even in asymptomatic individuals.


Assuntos
Coinfecção , Infecções por Papillomavirus/complicações , Infecções por Ureaplasma/complicações , Ureaplasma urealyticum/isolamento & purificação , Adulto , Feminino , Humanos , Pessoa de Meia-Idade , Infecções por Mycoplasma/complicações , Mycoplasma hominis , Papillomaviridae
9.
Microbes Environ ; 33(2): 151-161, 2018 Jul 04.
Artigo em Inglês | MEDLINE | ID: mdl-29863059

RESUMO

Various waterborne pathogens originate from human or animal feces and may cause severe gastroenteric outbreaks. Bacteroides spp. that exhibit strong host- or group-specificities are promising markers for identifying fecal sources and their origins. In the present study, 240 water samples were collected from two major aquaculture areas in Republic of Korea over a period of approximately 1 year, and the concentrations and occurrences of four host-specific Bacteroides markers (human, poultry, pig, and ruminant) were evaluated in the study areas. Host-specific Bacteroides markers were detected widely in the study areas, among which the poultry-specific Bacteroides marker was detected at the highest concentration (1.0-1.2 log10 copies L-1). During the sampling period, high concentrations of host-specific Bacteroides markers were detected between September and December 2015. The host-specific Bacteroides marker-combined geospatial map revealed the up-to-downstream gradient of fecal contamination, as well as the effects of land-use patterns on host-specific Bacteroides marker concentrations. In contrast to traditional bacterial indicators, the human-specific Bacteroides marker correlated with human specific pathogens, such as noroviruses (r=0.337; P<0.001). The present results indicate that host-specific Bacteroides genetic markers with an advanced geospatial analysis are useful for tracking fecal sources and associated pathogens in aquaculture areas.


Assuntos
Aquicultura/métodos , Bacteroides/genética , Monitoramento Ambiental/métodos , Microbiologia da Água , Poluição da Água/análise , Animais , Bacteroides/classificação , Bacteroides/isolamento & purificação , DNA Bacteriano/genética , Escherichia coli/classificação , Escherichia coli/genética , Escherichia coli/isolamento & purificação , Marcadores Genéticos/genética , Sistemas de Informação Geográfica , Especificidade de Hospedeiro , Humanos , Norovirus/classificação , Norovirus/genética , Norovirus/isolamento & purificação , RNA Viral/genética , República da Coreia , Estações do Ano , Análise Espacial
10.
ACS Nano ; 12(7): 7100-7108, 2018 07 24.
Artigo em Inglês | MEDLINE | ID: mdl-29920065

RESUMO

We report the development of a surface-enhanced Raman spectroscopy sensor chip by decorating gold nanoparticles (AuNPs) on ZnO nanorod (ZnO NR) arrays vertically grown on cellulose paper (C). We show that these chips can enhance the Raman signal by 1.25 × 107 with an excellent reproducibility of <6%. We show that we can measure trace amounts of human amniotic fluids of patients with subclinical intra-amniotic infection (IAI) and preterm delivery (PTD) using the chip in combination with a multivariate statistics-derived machine-learning-trained bioclassification method. We can detect the presence of prenatal diseases and identify the types of diseases from amniotic fluids with >92% clinical sensitivity and specificity. Our technology has the potential to be used for the early detection of prenatal diseases and can be adapted for point-of-care applications.


Assuntos
Papel , Complicações Infecciosas na Gravidez/diagnóstico , Nascimento Prematuro/diagnóstico , Diagnóstico Pré-Natal , Análise Espectral Raman/métodos , Líquido Amniótico/química , Celulose/química , Feminino , Ouro/química , Humanos , Nanopartículas Metálicas/química , Tamanho da Partícula , Gravidez , Propriedades de Superfície , Óxido de Zinco/química
11.
Menopause ; 24(7): 832-837, 2017 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-28291026

RESUMO

OBJECTIVE: The objective of this study was to assess the association between parity and insulin resistance in nondiabetic, postmenopausal women. METHODS: This cross-sectional study was conducted using data from the 2010 Korean National Health and Nutrition Examination Survey administered by the Korean Ministry of Health and Welfare. A total of 1,243 nondiabetic postmenopausal women were included in this study and subdivided into three groups according to parity (1-2, 3-4, and ≥5 live births). Insulin resistance was measured using the homeostasis model assessment of insulin resistance (HOMA-IR) index. The relationship between parity and insulin resistance was investigated using analysis of covariance. RESULTS: HOMA-IR showed a positive relationship with parity. Mean HOMA-IR (geometric mean and 95% CI) increased according to increasing parity group (1-2, 3-4, and ≥5 live births) after adjustment for age, smoking, alcohol consumption, exercise, education, income, and body mass index as follows: 2.1 (2.0-2.2) < 2.2 (2.1-2.3) < 2.5 (2.2-2.8) (P = 0.040 and P for trend = 0.012). In addition, this positive association was more apparent when insulin resistance was accompanied by obesity. The mean parity of the obese and insulin-resistant group was significantly higher than that of the nonobese insulin-sensitive group (3.6 ±â€Š0.1 vs 3.2 ±â€Š0.1, P = 0.047). CONCLUSIONS: Our study provides the first evidence that parity is significantly associated with insulin resistance in nondiabetic postmenopausal women. Further prospective longitudinal studies are needed to confirm the impact of parity on insulin resistance.


Assuntos
Resistência à Insulina/fisiologia , Paridade/fisiologia , Pós-Menopausa/fisiologia , Adulto , Idoso , Idoso de 80 Anos ou mais , Índice de Massa Corporal , Estudos Transversais , Feminino , Humanos , Insulina/sangue , Pessoa de Meia-Idade , Inquéritos Nutricionais , Obesidade/fisiopatologia , Gravidez , República da Coreia , Fatores de Risco
12.
Water Sci Technol ; 75(3-4): 978-986, 2017 02.
Artigo em Inglês | MEDLINE | ID: mdl-28234298

RESUMO

Identifying critical land-uses or source areas is important to prioritize resources for cost-effective stormwater management. This study investigated the use of information on ionic composition as a fingerprint to identify the source land-use of stormwater runoff. We used 12 ionic species in stormwater runoff monitored for a total of 20 storm events at five sites with different land-use compositions during the 2012-2014 wet seasons. A stepwise forward discriminant function analysis (DFA) with the jack-knifed cross validation approach was used to select ionic species that better discriminate the land-use of its source. Of the 12 ionic species, 9 species (K+, Mg2+, Na+, NH4+, Br-, Cl-, F-, NO2-, and SO42-) were selected for better performance of the DFA. The DFA successfully differentiated stormwater samples from urban, rural, and construction sites using concentrations of the ionic species (70%, 95%, and 91% of correct classification, respectively). Over 80% of the new data cases were correctly classified by the trained DFA model. When applied to data cases from a mixed land-use catchment and downstream, the DFA model showed the greater impact of urban areas and rural areas respectively in the earlier and later parts of a storm event.


Assuntos
Monitoramento Ambiental/métodos , Modelos Teóricos , Chuva , Movimentos da Água , Íons/análise , República da Coreia , Estações do Ano
13.
Biochem Biophys Res Commun ; 484(1): 218-223, 2017 02 26.
Artigo em Inglês | MEDLINE | ID: mdl-27998772

RESUMO

OBJECTIVE: There is evidence that the mineral zinc is involved in the apoptotic cell death of various carcinoma cells. In this study, we aim to determine whether zinc in the form of CIZAR induces apoptosis in cervical carcinoma cells by increasing intracellular zinc concentration. STUDY DESIGN: CaSki and HeLa cervical carcinoma cells and HPV-16 DNA-transformed keratinocyte (CRL2404) were treated with different concentrations of CIZAR. The cell viability test was carried out, the intracellular level of zinc was determined, and apoptosis was confirmed by flow cytometry after propidium iodide (PI) staining and fluorescence microscopy under DAPI staining. The expression of cell-cycle regulators was analyzed by Western blot, including the knock down of p53 and expression of HPV E6 and E7 genes by RT-PCR. RESULTS: Intracellular zinc accumulation induced the down-regulation of E6/E7 proteins through targeting of the specific transcriptional factors in the upstream regulatory region. p53 was induced after CIZAR treatment and p53-dependent apoptosis did not occur after knock down by p53 siRNA. In cervical carcinoma cells, regardless of HPV-infection, CIZAR induces apoptosis by the activation of the p53-independent pathways through the up-regulation of p21waf1, the down-regulation of c-Myc, and by decreasing the Bcl-2/Bax ratio. CONCLUSIONS: CIZAR induces apoptosis not only through the restoration of p53/Rb-dependent pathways in HPV-positive cells, but also through the activation of p53/Rb-independent pathways and the mitochondrial death-signal pathway in cervical carcinoma cells regardless of HPV-infection.


Assuntos
Apoptose/efeitos dos fármacos , Proteína Supressora de Tumor p53/metabolismo , Neoplasias do Colo do Útero/patologia , Zinco/farmacologia , Alphapapillomavirus/genética , Linhagem Celular Tumoral , Inibidor de Quinase Dependente de Ciclina p21/metabolismo , Regulação para Baixo , Feminino , Genes Virais , Genes myc , Humanos , Regulação para Cima , Neoplasias do Colo do Útero/metabolismo , Neoplasias do Colo do Útero/virologia
14.
Medicine (Baltimore) ; 95(39): e4981, 2016 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-27684850

RESUMO

We compared results using unidirectional barbed sutures and conventional sutures for vaginal cuff closure during total laparoscopic hysterectomy (TLH).The electronic medical records and surgical videos of 170 patients who underwent TLH between January 2013 and March 2015 at Uijeong-bu St. Mary's Hospital of Catholic University of Korea were reviewed. Vaginal cuffs were closed using the 2-layer continuous running technique with unidirectional barbed sutures (V-Loc; Covidien, Mansfield, MA) in 64 patients and with polycolic acid Vicryl; Ethicon, Somerville, NJ sutures in 106 patients. Procedure time, clinical characteristics, and postoperative complications were compared between the 2 study groups. There were no differences in clinical characteristics (age, body mass index, and demographic data) between groups. The mean suturing time was significantly reduced in the barbed group (7.2 vs 12.2 minutes; P < 0.001), although the mean number of stitches was greater than in the Vicryl group (14.1 vs 12.3, P < 0.001). Perioperative complications, including episodes of vaginal bleeding, vaginal cuff cellulitis, and postoperative fever, did not differ between groups. There were no instances of vaginal cuff dehiscence in either group. Unidirectional barbed sutures can be used safely to reduce procedure time and surgical difficulty relative to conventional sutures in laparoscopic vaginal cuff closure.


Assuntos
Histerectomia/métodos , Laparoscopia/métodos , Técnicas de Sutura , Suturas , Adulto , Feminino , Humanos , Pessoa de Meia-Idade , Duração da Cirurgia , Poliglactina 910/uso terapêutico , Complicações Pós-Operatórias/etiologia , República da Coreia , Estudos Retrospectivos , Resultado do Tratamento , Vagina/cirurgia
15.
Sci Total Environ ; 569-570: 291-299, 2016 Nov 01.
Artigo em Inglês | MEDLINE | ID: mdl-27343948

RESUMO

Although norovirus outbreaks are well-recognized to have strong winter seasonality relevant to low temperature and humidity, the role of artificial human-made features within geographical areas in norovirus outbreaks has rarely been studied. The aim of this study is to assess the natural and human-made environmental factors favoring the occurrence of norovirus outbreaks using nationwide surveillance data. We used a geographic information system and binary response models to examine whether the norovirus outbreaks are spatially patterned and whether these patterns are associated with specific environmental variables including service levels of water supply and sanitation systems and land-use types. The results showed that small-scale low-tech local sewage treatment plants and winter sports areas were statistically significant factors favoring norovirus outbreaks. Compactness of the land development also affected the occurrence of norovirus outbreaks; transportation, water, and forest land-uses were less favored for effective transmission of norovirus, while commercial areas were associated with an increased rate of norovirus outbreaks. We observed associations of norovirus outbreaks with various outcomes of human activities, including discharge of poorly treated sewage, overcrowding of people during winter season, and compactness of land development, which might help prioritize target regions and strategies for the management of norovirus outbreaks.


Assuntos
Infecções por Caliciviridae/epidemiologia , Surtos de Doenças , Gastroenterite/epidemiologia , Sistemas de Informação Geográfica , Norovirus/fisiologia , Infecções por Caliciviridae/virologia , Gastroenterite/virologia , Humanos , Modelos Teóricos , República da Coreia/epidemiologia
16.
Acta Obstet Gynecol Scand ; 95(7): 829-35, 2016 07.
Artigo em Inglês | MEDLINE | ID: mdl-26988751

RESUMO

INTRODUCTION: The aim of this study was to investigate the association between Toll-like receptor 2 (TLR2) gene polymorphisms and human papillomavirus (HPV)-related cervical neoplasia in Korean women. MATERIAL AND METHODS: Peripheral blood samples collected from 127 patients with HPV-related cervical neoplasia and 175 healthy women were genotyped for the TLR2 -16934, +1350, intron1, and 3' untranslated region (UTR) polymorphisms using the polymerase chain reaction and restriction fragment length polymorphism method. RESULTS: The TLR2 -16934 A/A, intron1 A/A, and +1350 T/C genotypes were more frequent in patients than in controls [odds ratio (OR) = 2.1, 95% CI = 1.302-3.475, p = 0.002; OR = 1.9, 95% CI = 1.168-3.169, p = 0.010; and OR = 1.9, 95% CI = 1.211-3.123, p = 0.006, respectively]. The frequencies of the TLR2 + 1350 C and 3'UTR G alleles were also higher in patients (OR = 2.0, 95% CI = 1.236-3.121, p = 0.004 and OR = 1.7, 95% CI = 1.005-3.076, p = 0.046, respectively). The genotype frequencies of TLR2 -16934 A/A and intron1 A/A increased with increasing oncogenic risk of the HPV genotype, as follows. low-risk type < high-risk type < HPV-16 and/or HPV-18 type (p = 0.008). CONCLUSIONS: Our study provides the first evidence that TLR2 gene polymorphisms are associated with high-risk type HPV-related cervical neoplasia and may play an important role in susceptibility to HPV infection. Further large-scale and functional studies are needed to confirm the role of TLR2 gene polymorphisms in HPV-related cervical neoplasia.


Assuntos
Neoplasia Intraepitelial Cervical/genética , Papillomaviridae/genética , Infecções por Papillomavirus/genética , Receptor Toll-Like 9/genética , Neoplasias do Colo do Útero/genética , Adulto , Grupo com Ancestrais do Continente Asiático , Estudos de Casos e Controles , Neoplasia Intraepitelial Cervical/sangue , Feminino , Genótipo , Humanos , Infecções por Papillomavirus/sangue , Polimorfismo de Nucleotídeo Único , República da Coreia , Neoplasias do Colo do Útero/sangue
17.
Environ Sci Pollut Res Int ; 23(10): 9774-90, 2016 May.
Artigo em Inglês | MEDLINE | ID: mdl-26850099

RESUMO

While identification of critical pollutant sources is the key initial step for cost-effective runoff management, it is challenging due to the highly uncertain nature of runoff pollution, especially during a storm event. To identify critical sources and their quantitative contributions to runoff pollution (especially focusing on phosphorous), two ordination methods were used in this study: principal component analysis (PCA) and positive matrix factorization (PMF). For the ordination analyses, we used runoff quality data for 14 storm events, including data for phosphorus, 11 heavy metal species, and eight ionic species measured at the outlets of subcatchments with different land use compositions in a mixed land use watershed. Five factors as sources of runoff pollutants were identified by PCA: agrochemicals, groundwater, native soils, domestic sewage, and urban sources (building materials and automotive activities). PMF identified similar factors to those identified by PCA, with more detailed source mechanisms for groundwater (i.e., nitrate leaching and cation exchange) and urban sources (vehicle components/motor oils/building materials and vehicle exhausts), confirming the sources identified by PCA. PMF was further used to quantify contributions of the identified sources to the water quality. Based on the results, agrochemicals and automotive activities were the two dominant and ubiquitous phosphorus sources (39-61 and 16-47 %, respectively) in the study area, regardless of land use types.


Assuntos
Poluentes Químicos da Água/análise , Agricultura , Geografia , Fósforo/análise , Chuva , República da Coreia , Esgotos , Razão Sinal-Ruído , Movimentos da Água , Qualidade da Água
18.
Gynecol Obstet Invest ; 80(1): 21-5, 2015.
Artigo em Inglês | MEDLINE | ID: mdl-25791173

RESUMO

AIMS: The objective of the current study was to evaluate the efficacy and feasibility of fibrin sealant (Tisseel®) in the loop electrosurgical excision procedure (LEEP) for cervical intraepithelial neoplasia (CIN 2 or 3). METHODS: We designed a single-blind, prospective, randomized study in 40 consecutive women undergoing LEEP for CIN 2 or 3 at our institute. Two milliliters of fibrin sealant (Tisseel) was applied to the uterine cervix of 20 women immediately after LEEP surgery (treatment group). We evaluated abdominal pain, vaginal bleeding, vaginal discharge and impairment in daily living after 1 week using visual analogue scale questionnaires and compared the results with those of 20 women who did not receive fibrin sealant (control group). RESULTS: Among 40 women who returned for a follow-up 1 week after LEEP, 25 women (62.5%) reported at least one moderate to severe postprocedural symptom. The mean duration of moderate to severe vaginal bleeding and impairment in daily living during postoperative week 1 for the treatment group and the control group was 0.3 ± 0.80 versus 1.7 ± 2.36 days (p = 0.015) and 0.9 ± 1.37 versus 3.00 ± 2.62 days (p = 0.060), respectively. CONCLUSION: Intraoperative application of fibrin sealant (Tisseel) in LEEP can decrease postoperative vaginal bleeding and impairment in daily living.


Assuntos
Neoplasia Intraepitelial Cervical/cirurgia , Conização/métodos , Eletrocirurgia/métodos , Adesivo Tecidual de Fibrina/uso terapêutico , Neoplasias do Colo do Útero/cirurgia , Dor Abdominal , Atividades Cotidianas , Adulto , Feminino , Humanos , Pessoa de Meia-Idade , Projetos Piloto , Complicações Pós-Operatórias/prevenção & controle , Estudos Prospectivos , Método Simples-Cego , Inquéritos e Questionários , Resultado do Tratamento , Hemorragia Uterina , Descarga Vaginal
19.
Int J Med Sci ; 9(8): 698-703, 2012.
Artigo em Inglês | MEDLINE | ID: mdl-23091406

RESUMO

OBJECTIVE: To evaluate the correlation tendency between abnormal findings of digital cervicography and cervical pathology at private clinics in Korea. METHODS: Abnormal finding of digital cervicography performed at private clinics in Korea between January 1, 2010 and May 31, 2012 were analysed retrospectively. The patient's age, abnormal findings of digital cervicography, cervical cytology, human papillomaviru (HPV) test and cervical pathology were investigated and the rate of agreement between abnormal finding of digital cervicography and cervical pathology results was calculated. Abnormal findings of digital cervicography were divided into 4 categories: atypical, compatible with CIN1, compatible with CIN2/3 and compatible with cancer. RESULTS: The study group was composed of 1547 women with a mean (range) age of 37.4 (14-91 years). The agreement rate between abnormal findings of digital cervicography and cervical pathology was 52.0% in "compatible with CIN1", 78.9% in "compatible with CIN2/3", and 90.2% in "compatible with cancer". CONCLUSIONS: Abnormal findings of digital cervicography were highly concordant with cervical intraepithelial neoplasia (CIN) and cancer examined at outpatient clinics in Korea. Therefore, abnormal interpretations of digital cervicography can be used as an excellent auxiliary technique with cervical cytology for CIN and cancer.


Assuntos
Instituições de Assistência Ambulatorial/organização & administração , Setor Privado , Neoplasias do Colo do Útero/diagnóstico , Adolescente , Adulto , Idoso , Idoso de 80 Anos ou mais , Feminino , Humanos , Pessoa de Meia-Idade , República da Coreia , Adulto Jovem
20.
Int J Gynecol Cancer ; 22(4): 686-91, 2012 May.
Artigo em Inglês | MEDLINE | ID: mdl-22398707

RESUMO

OBJECTIVE: This study aimed to determine the incidence and risk factors of lower-extremity lymphedema (LEL) in women who had radical surgery with or without adjuvant radiotherapy for International Federation of Gynecology and Obstetrics (FIGO) stage I to stage IIA cervical cancer. METHODS: The medical records were reviewed retrospectively on patients with histologically confirmed FIGO stage I to IIA cervical cancer. Lower-extremity lymphedema-related medical problems such as peripheral vascular disease, congestive heart failure, or chronic renal disease were excluded. A logistic regression analysis was used to examine the relationship between variable clinical characteristics and development of LEL. RESULTS: We evaluated 707 patients. Of the 707 patients evaluated, we excluded 92 patients who had received radiotherapy as the initial therapy and 19 patients with LEL related to medical problems. Seventy-five patients (12.6%) developed LEL. The incidence was high in patients with adjuvant radiotherapy (odds ratio, 3.47; 95% confidence interval, 2.086-5.788; P = 0.000), with 78.7% of the patients with LEL having developed the condition within 3 years after initial treatment. CONCLUSIONS: Adjuvant radiotherapy was significantly associated with development of LEL in women who had undergone radical surgery with lymphadenectomy for FIGO stage I to stage IIA cervical cancer. The possibility for the occurrence of LEL must be fully explained before treatment and patients should be provided with the appropriate preventive education. Further prospective studies are needed to confirm the incidence and risk factors for LEL.


Assuntos
Histerectomia/efeitos adversos , Extremidade Inferior/patologia , Excisão de Linfonodo/efeitos adversos , Linfedema/etiologia , Radioterapia Adjuvante/efeitos adversos , Neoplasias do Colo do Útero/complicações , Adulto , Feminino , Seguimentos , Humanos , Incidência , Extremidade Inferior/efeitos da radiação , Extremidade Inferior/cirurgia , Metástase Linfática , Linfedema/epidemiologia , Pessoa de Meia-Idade , Estadiamento de Neoplasias , Cuidados Pós-Operatórios , Prognóstico , República da Coreia/epidemiologia , Estudos Retrospectivos , Fatores de Risco , Neoplasias do Colo do Útero/terapia
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