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1.
J Clin Pediatr Dent ; 45(3): 186-192, 2021 Jul 01.
Artigo em Inglês | MEDLINE | ID: mdl-34192754

RESUMO

OBJECTIVE: To compare the shear bond strength (SBS) after thermocycling of four universal adhesives applied in self-etch mode on dentin and etch-and-rinse mode on enamel. STUDY DESIGN: Flat 144 buccal or lingual dentin and enamel surfaces from 72 non-carious primary molars were prepared. Samples were segregated into 12 groups (n=12): Adper Single Bond 2 etch-and-rinse (SB_T) and Clearfil SE Bond self-etch (SE_S) applied to enamel and dentin served as controls. Scotch Bond Universal Adhesive (SBU), Clearfil S3 Bond Universal Adhesive (SEU), Tetric N-Bond Universal Adhesive (TEN), and All Bond Universal (BIS) were applied in etch-and-rinse mode to enamel and in self-etch mode to dentin. They were thermocycled for 5000 cycles. SBS testing and the evaluation of fracture mode were performed. RESULTS: SB_T showed statistically higher SBS than other adhesive groups using etch-and-rinse mode on enamel. SE_S and BIS had statistically higher SBS than other adhesive groups using self-etch mode on dentin. Mixed failure was the most common failure mode in each group. CONCLUSION: The universal adhesives did not show higher SBS than SB_T when using etch-and-rinse on enamel. All universal adhesives showed higher SBS than SB_T and had SBS similar to SE_S, except SBU when using self-etch mode on dentin.


Assuntos
Colagem Dentária , Adesivos Dentinários , Esmalte Dentário , Dentina , Humanos , Teste de Materiais , Cimentos de Resina , Resistência ao Cisalhamento , Dente Decíduo
2.
Int Neurourol J ; 23(Suppl 1): S22-31, 2019 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-30832464

RESUMO

PURPOSE: This study aimed to investigate the effects of single-bout exercise on mitochondrial function, dynamics (fusion, fission), and mitophagy in cardiac and skeletal muscles. METHODS: Fischer 344 rats (4 months old) were randomly divided into the control (CON) or acute exercise (EX) group (n=10 each). The rats performed a single bout of treadmill exercise for 60 minutes. Mitochondrial function (e.g., O2 respiration, H2O2 emission, Ca2+ retention capacity), mitochondrial fusion (e.g., Mfn1, Mfn2, Opa1), mitochondrial fission (e.g., Drp1, Fis1), and mitophagy (e.g., Parkin, Pink1, LC3II, Bnip3) were measured in permeabilized cardiac (e.g., left ventricle) and skeletal (e.g., soleus, white gastrocnemius) muscles. RESULTS: Mitochondrial O2 respiration and Ca2+ retention capacity were significantly increased in all tissues of the EX group compared with the CON group. Mitochondrial H2O2 emissions showed tissue-specific results; the emissions showed no significant differences in the left ventricle or soleus (type I fibers) but was significantly increased in the white gastrocnemius (type II fibers) after acute exercise. Mitochondrial fusion and fission were not altered in any tissues of the EX group. Mitophagy showed tissue-specific differences: It was not changed in the left ventricle or white gastrocnemius, whereas Parkin and LC3II were significantly elevated in the soleus muscle. CONCLUSION: A single bout of aerobic exercise may improve mitochondrial function (e.g., O2 respiration and Ca2+ retention capacity) in the heart and skeletal muscles without changes in mitochondrial dynamics or mitophagy.

3.
J Exerc Rehabil ; 14(5): 758-764, 2018 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-30443520

RESUMO

The present study aimed to comparatively analyze the effects of the whole-body vibration exercise health promotion program on the agility, the quick reaction ability, and the flexibility of the test-takers. Total 30 test-takers had been prepared for the practical test as the subjects. They were divided into the three groups: the practical college entrance examination group with whole-body vibration exercise, the practical college entrance examination group, and the control group. The measurements of each item were made before and after the program applications of the side steps, the standing long jump, the standing high jump, the sitting trunk flexion, and the trunk forward flexion for 60 min 3 times a week. The side steps showed the significant differences among the groups. Although the standing long jump did not show any significant differences among the groups, the standing high jump showed the significant differences among the groups. Although the sitting trunk flexion showed the significant differences among the groups, the trunk forward flexion did not show any significant differences among the groups. But, the practical college entrance examination program group which carried out the whole-body vibration exercise at the same time showed the highest average value compared to the other groups. It was proven that, to the test-takers who had been preparing for the preparing examination for applying to a physical education-affiliated department, the whole-body vibration exercise program is helpful to the promotion of the health, including the agility, the quick reaction ability, the flexibility, etc.

4.
J Exerc Rehabil ; 14(5): 791-801, 2018 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-30443525

RESUMO

The purpose of this study was to investigate the effects of different weight reduction methods on the body composition characteristics, cardiopulmonary functions, and health promotion of elite bodybuilding athletes in a comprehensive manner. For this purpose, the study analyzed the effects of two different weight reduction methods on 25 elite bodybuilding athletes registered at the Bodybuilding Association over a period of 6 weeks. There were interactive effects on macronutrients, macrominerals, and antioxidants according to weight reduction methods as the experiment group (6%±2%) maintained a certain amount of food intakes across various nutrients including energy intakes or made a slow decrease it, whereas the traditional group (16%±4%) made a sharp decrease in it due to relatively greater weight reduction than the experiment group and thus showed pattern differences from it. As for immunity variables, there was an increase to certain cytokines despite the traditional high weight reduction method, but it caused no excessive reduction of immunity or rapid decrease to certain factors of body composition. In addition, there were no significant differences in main and interactive effects on the one-repetition maximum of bench press and squat, which are two functional indicators of health promotion. These findings imply that the low weight reduction method within the range of 6%±2% minimized or maintained changes to muscle circumference or mass while causing no improvement to muscular strength.

5.
Sci Rep ; 7(1): 12037, 2017 09 20.
Artigo em Inglês | MEDLINE | ID: mdl-28931866

RESUMO

Lithium-metal-based batteries, owing to the extremely high specific energy, have been attracting intense interests as post-Li-ion batteries. However, their main drawback is that consumption/de-activation of lithium metal can be accelerated when O2 or S used in the cathode crosses over to the metal, reducing the lifetime of the batteries. In use of ceramic solid state electrolyte (SSE) separator, despite the capability of gas blocking, thick and heavy plates (~0.3 mm) are necessitated to compensate its mechanical fragility, which ruin the high specific energy of the batteries. Here, we demonstrate fabrication of a new membrane made of micron-sized SSE particles as Li-ion channels embedded in polymer matrix, which enable both high Li-ion conduction and gas-impermeability. Bimodal surface-modification was used to control the energy of the particle/polymer interface, which consequently allowed channel formation via a simple one-step solution process. The practical cell with the new membrane provides a cell-specific energy of over 500 Wh kg-1, which is the highest values ever reported.

6.
Sci Adv ; 2(8): e1600418, 2016 08.
Artigo em Inglês | MEDLINE | ID: mdl-27493994

RESUMO

Recent advances in materials, mechanics, and electronic device design are rapidly establishing the foundations for health monitoring technologies that have "skin-like" properties, with options in chronic (weeks) integration with the epidermis. The resulting capabilities in physiological sensing greatly exceed those possible with conventional hard electronic systems, such as those found in wrist-mounted wearables, because of the intimate skin interface. However, most examples of such emerging classes of devices require batteries and/or hard-wired connections to enable operation. The work reported here introduces active optoelectronic systems that function without batteries and in an entirely wireless mode, with examples in thin, stretchable platforms designed for multiwavelength optical characterization of the skin. Magnetic inductive coupling and near-field communication (NFC) schemes deliver power to multicolored light-emitting diodes and extract digital data from integrated photodetectors in ways that are compatible with standard NFC-enabled platforms, such as smartphones and tablet computers. Examples in the monitoring of heart rate and temporal dynamics of arterial blood flow, in quantifying tissue oxygenation and ultraviolet dosimetry, and in performing four-color spectroscopic evaluation of the skin demonstrate the versatility of these concepts. The results have potential relevance in both hospital care and at-home diagnostics.


Assuntos
Técnicas Biossensoriais , Eletrônica , Fenômenos Fisiológicos da Pele , Pele , Tecnologia sem Fio , Técnicas Biossensoriais/instrumentação , Técnicas Biossensoriais/métodos , Pressão Sanguínea , Eletrônica/instrumentação , Eletrônica/métodos , Epiderme/fisiologia , Frequência Cardíaca , Oximetria/instrumentação , Oximetria/métodos , Dosímetros de Radiação , Fluxo Sanguíneo Regional
7.
Cell Mol Biol Lett ; 20(4): 535-48, 2015 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-26124051

RESUMO

The high frequency of intrinsic resistance to TNF-related apoptosisinducing ligand (TRAIL) in tumor cell lines has necessitated the development of strategies to sensitize tumors to TRAIL-induced apoptosis. We previously showed that elevated pressure applied as a mechanical stressor enhanced TRAIL-mediated apoptosis in human lung carcinoma cells in vitro and in vivo. This study focused on the effect of elevated pressure on the sensitization of TRAIL-resistant cells and the underlying mechanism. We observed elevated pressure-induced sensitization to TRAIL-mediated apoptosis in Hep3B cells, accompanied by the activation of several caspases and the mitochondrial signaling pathway. Interestingly, the enhanced apoptosis induced by elevated pressure was correlated with suppression of extracellular signal-regulated protein kinase 1 and 2 (ERK1/2) phosphorylation and CREB without any change to other MAPKs. Phosphorylation of Bcl-2-associated death promoter (BAD) also decreased, leading to inhibition of the mitochondrial pathway. To confirm whether the activation of pERK1/2 plays a key role in the TRAIL-sensitizing effect of elevated pressure, Hep3B cells were pre-treated with the ERK1/2-specific inhibitor PD98059 instead of elevated pressure. Co-treatment with PD98059 and TRAIL augmented TRAIL-induced apoptosis and decreased BAD phosphorylation. The inhibition of ERK1/2 activation by elevated pressure and PD98059 also reduced BH3 interacting-domain death agonist (BID), thereby amplifying apoptotic stress at the mitochondrial level. Our results suggest that elevated pressure enhances TRAIL-induced apoptosis of Hep3B cells via specific suppression of ERK1/2 activation among MAPKs.


Assuntos
Apoptose/efeitos dos fármacos , Carcinoma Hepatocelular/patologia , Neoplasias Hepáticas/patologia , Proteína Quinase 1 Ativada por Mitógeno/metabolismo , Ligante Indutor de Apoptose Relacionado a TNF/farmacologia , Apoptose/fisiologia , Carcinoma Hepatocelular/tratamento farmacológico , Carcinoma Hepatocelular/metabolismo , Caspases/metabolismo , Linhagem Celular Tumoral/efeitos dos fármacos , Proteína de Ligação ao Elemento de Resposta ao AMP Cíclico/metabolismo , Resistencia a Medicamentos Antineoplásicos , Flavonoides/farmacologia , Humanos , Neoplasias Hepáticas/tratamento farmacológico , Neoplasias Hepáticas/metabolismo , Sistema de Sinalização das MAP Quinases/efeitos dos fármacos , Proteína Quinase 1 Ativada por Mitógeno/antagonistas & inibidores , Proteína Quinase 3 Ativada por Mitógeno/antagonistas & inibidores , Proteína Quinase 3 Ativada por Mitógeno/metabolismo , Pressão , Inibidores de Proteínas Quinases/farmacologia , Ligante Indutor de Apoptose Relacionado a TNF/genética , Ligante Indutor de Apoptose Relacionado a TNF/metabolismo , Proteína de Morte Celular Associada a bcl/metabolismo
8.
Apoptosis ; 15(12): 1517-28, 2010 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-20623193

RESUMO

TNF-related apoptosis-inducing ligand (TRAIL, Apo2L) is a promising anticancer agent with high specificity for cancer cells. Many strategies have been proposed to enhance the sensitivity of cancer cells to TRAIL-mediated apoptosis, including the use of combination treatment with conventional cancer therapies. However, few reports have evaluated the effects of TRAIL in combination with mechanical stress, which can also cause apoptosis of cancer cells. In the present study, we describe a custom-designed culture system that delivers two atmospheres of elevated pressure (EP) by using compressed air, and which enhances the sensitivity of cancer cells to TRAIL-mediated apoptosis. The combination of TRAIL and EP significantly increased apoptosis of human H460 lung cancer cells more than hyperbaric normoxia or normobaric mild hyperoxia. EP-potentiating TRAIL-mediated apoptosis of H460 cells was accompanied by up-regulated death receptor 5 (DR5), activation of caspases, decreased mitochondrial membrane potential, and reactive oxygen species production. We also observed EP-induced sensitization of TRAIL-mediated apoptosis in other cancer cell types. In contrast, human normal cells showed no DNA damage or cell death when exposed to the combined treatment. In a chicken chorioallantoic membrane model, EP enhanced TRAIL-mediated apoptosis of tumors that developed from transplanted H460 cells. Collectively, EP enhanced TRAIL-induced apoptosis of human lung carcinoma cells in vitro and in vivo. These findings suggest that EP is a mechanical and physiological stimulus that might have utility as a sensitizing tool for cancer therapy.


Assuntos
Antineoplásicos/farmacologia , Apoptose , Potencial da Membrana Mitocondrial/efeitos dos fármacos , Receptores do Ligante Indutor de Apoptose Relacionado a TNF/metabolismo , Proteínas Recombinantes , Ligante Indutor de Apoptose Relacionado a TNF , Animais , Antineoplásicos/uso terapêutico , Apoptose/efeitos dos fármacos , Apoptose/fisiologia , Carcinoma Pulmonar de Células não Pequenas/tratamento farmacológico , Carcinoma Pulmonar de Células não Pequenas/metabolismo , Carcinoma Pulmonar de Células não Pequenas/patologia , Caspases/metabolismo , Linhagem Celular Tumoral , Embrião de Galinha , Terapia Combinada , Feminino , Feto , Humanos , Peptídeos e Proteínas de Sinalização Intracelular/metabolismo , Neoplasias Pulmonares/tratamento farmacológico , Neoplasias Pulmonares/metabolismo , Neoplasias Pulmonares/patologia , Potencial da Membrana Mitocondrial/fisiologia , Estresse Oxidativo , Espécies Reativas de Oxigênio/metabolismo , Proteínas Recombinantes/genética , Proteínas Recombinantes/farmacologia , Proteínas Recombinantes/uso terapêutico , Transdução de Sinais/efeitos dos fármacos , Transdução de Sinais/fisiologia , Estresse Mecânico , Ligante Indutor de Apoptose Relacionado a TNF/genética , Ligante Indutor de Apoptose Relacionado a TNF/farmacologia , Ligante Indutor de Apoptose Relacionado a TNF/uso terapêutico
9.
Biochem Biophys Res Commun ; 399(1): 91-7, 2010 Aug 13.
Artigo em Inglês | MEDLINE | ID: mdl-20643104

RESUMO

Intensive cancer therapy strategies have thus far focused on sensitizing cancer cells to anticancer drug-mediated apoptosis to overcome drug resistance, and this strategy has led to more effective cancer therapeutics. Cisplatin (cis-diamminedichloroplatinum(II), CDDP) is an effective anticancer drug used to treat many types of cancer, including non-small cell lung carcinoma (NSCLC), and can be used in combination with various chemicals to enhance cancer cell apoptosis. Here, we introduce the use of elevated pressure (EP) in combination with CDDP for cancer treatment and explore the effects of EP on CDDP-mediated apoptosis in NSCLC cells. Our findings demonstrate that preconditioning NSCLC cells with EP sensitizes cells for CDDP-induced apoptosis. Enhanced apoptosis was dependent on p53 and HO-1 expression, and was associated with increased DNA damage and down-regulation of genes involved in nucleotide excision repair. The transcriptional levels of transporter proteins indicated that the mechanism by which EP-induced CDDP sensitization was intracellular drug accumulation. The protein levels of some antioxidants, such as hemeoxygenase-1 (HO-1), glutathione (GSH) and glutathione peroxidase (Gpx), were decreased in A549 cells exposed to EP via the down-regulation of the transcription factor nuclear factor (erythroid-derived 2)-like 2 (Nrf-2). Furthermore, normal human fibroblasts were resistant to EP treatment, with no elevated DNA damage or apoptosis. Collectively, these data show that administration of EP is a potential adjuvant tool for CDDP-based chemosensitivity of lung cancer cells that may reduce drug resistance.


Assuntos
Antineoplásicos/uso terapêutico , Carcinoma Pulmonar de Células não Pequenas/terapia , Cisplatino/uso terapêutico , Resistencia a Medicamentos Antineoplásicos , Neoplasias Pulmonares/terapia , Pressão , Estresse Mecânico , Apoptose , Carcinoma Pulmonar de Células não Pequenas/tratamento farmacológico , Linhagem Celular Tumoral , Dano ao DNA , Reparo do DNA/genética , Heme Oxigenase-1/antagonistas & inibidores , Heme Oxigenase-1/genética , Humanos , Neoplasias Pulmonares/tratamento farmacológico , Fator 2 Relacionado a NF-E2/antagonistas & inibidores
10.
J Korean Med Sci ; 25(5): 684-90, 2010 May.
Artigo em Inglês | MEDLINE | ID: mdl-20436702

RESUMO

The purpose of our study was to determine the most accurate analytic method to define in vitro chemosensitivity, using clinical response as reference standard in prospective clinical trial, and to assess accuracy of adenosine triphosphate-based chemotherapy response assay (ATP-CRA). Forty-eight patients with chemo-naïve, histologically confirmed, locally advanced or metastatic gastric cancer were enrolled for the study and were treated with combination chemotherapy of paclitaxel 175 mg/m(2) and cisplatin 75 mg/m(2) for maximum of six cycles after obtaining specimen for ATP-CRA. We performed the receiver operator characteristic curve analysis using patient responses by WHO criteria and ATP-CRA results to define the method with the highest accuracy. Median progression free survival was 4.2 months (95% confidence interval [CI]: 3.4-5.0) and median overall survival was 11.8 months (95% CI: 9.7-13.8) for all enrolled patients. Chemosensitivity index method yielded highest accuracy of 77.8% by ROC curve analysis, and the specificity, sensitivity, positive and negative predictive values were 95.7%, 46.2%, 85.7%, and 75.9%. In vitro chemosensitive group showed higher response rate (85.7% vs. 24.1%) (P=0.005) compared to chemoresistant group. ATP-CRA could predict clinical response to paclitaxel and cisplatin chemotherapy with high accuracy in advanced gastric cancer patients. Our study supports the use of ATP-CRA in further validation studies.


Assuntos
Trifosfato de Adenosina/análise , Protocolos de Quimioterapia Combinada Antineoplásica/administração & dosagem , Ensaios de Seleção de Medicamentos Antitumorais/métodos , Neoplasias Gástricas/diagnóstico , Neoplasias Gástricas/tratamento farmacológico , Adulto , Idoso , Antineoplásicos Fitogênicos/administração & dosagem , Cisplatino/administração & dosagem , Feminino , Humanos , Coreia (Geográfico) , Masculino , Pessoa de Meia-Idade , Avaliação de Resultados em Cuidados de Saúde/métodos , Paclitaxel/administração & dosagem , Reprodutibilidade dos Testes , Sensibilidade e Especificidade , Neoplasias Gástricas/metabolismo , Resultado do Tratamento
11.
Cell Stress Chaperones ; 13(4): 447-58, 2008 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-18465208

RESUMO

The pressure during hyperbaric oxygen treatment may increase oxygen toxicity via an augmented oxygen pressure in the gas. Nevertheless, only a few reports have been published on the effect of cells grown under 2 atmospheric absolute (ATA) pressure. To evaluate the effect of pressure on oxygen toxicity and to study effects in addition to oxygen toxicity, we designed an experiment to compare the effects of normobaric mild hyperoxia (NMH, 40% oxygen) and hyperbaric air condition (HA, air with 2 ATA) on human diploid fibroblasts (HDF) in a hyperbaric incubator. HDFs in both the NMH and the HA condition had a similar oxidative stress response and exhibited premature senescence. To investigate differences in gene profiling in cells grown in the NMH and HA conditions, samples from cells exposed to each condition were applied to microarrays. We found no expression difference in genes related to aging and deoxyribonucleic acid damage, but the expression of genes including cell adhesion, stress response, and transcription were significantly increased in fibroblasts that were responsive to pressure. Among 26 statistically reliable genes, the expression of apoptosis related genes such as ADAM22, Bax, BCL2L14, and UBD, as well as tumor suppressor-related genes like Axin2 and ATF, and also mitogen-activated protein kinase-related genes like mitogen-activated protein kinase kinase kinase 1, histamine receptor, and RAB24, were significantly changed in cells responsive to pressure-induced oxidative stress.


Assuntos
Senilidade Prematura/patologia , Pressão do Ar , Senescência Celular/efeitos dos fármacos , Diploide , Fibroblastos/citologia , Oxigênio/farmacologia , Estresse Fisiológico/efeitos dos fármacos , Proteínas de Ciclo Celular/metabolismo , Proliferação de Células/efeitos dos fármacos , Forma Celular/efeitos dos fármacos , Meios de Cultura , Dano ao DNA , Fibroblastos/efeitos dos fármacos , Fibroblastos/enzimologia , Regulação da Expressão Gênica/efeitos dos fármacos , Proteínas de Choque Térmico/metabolismo , Humanos , Análise de Sequência com Séries de Oligonucleotídeos , Estresse Oxidativo/efeitos dos fármacos , Reprodutibilidade dos Testes , Reação em Cadeia da Polimerase Via Transcriptase Reversa , Telômero/metabolismo , Fatores de Tempo , beta-Galactosidase/metabolismo
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