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1.
Artigo em Inglês | MEDLINE | ID: mdl-32590442

RESUMO

BACKGROUND: As the largest and unhealthiest population in American history enrolls as Medicare beneficiaries, it is vital for primary care providers to understand how to maximize Medicare wellness provisions. The Baby Boomer population has been documented to have the highest chronic disease prevalence related to preventable lifestyle behaviors. Perpetual unhealthy lifestyle behaviors associated with chronic disease prevalence are detrimental to life quality and the American Medicare resource structure. Since 2011, the Affordable Care Act provisions have included free wellness visits designed to prevent disease for Medicare beneficiaries, who continue to grossly underuse these services. OBJECTIVES: This systematic review was conducted to evaluate the quality, level, and strength of evidence regarding Medicare wellness service efficacy on related health outcomes. DATA SOURCES: The methodology adhered to the Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) guidelines for selection of 21 research-based articles included in the analysis. Results from the 21 eligible studies revealed low research quality and vigor; therefore, lacking causality and generalizability of medicare wellness visit (MWV) efficacy on health promotion outcomes. CONCLUSIONS: The evidence is focused on how MWVs are affecting preventive care utilization instead of patient health outcomes. In the interest of reducing chronic disease prevalence and the economic burden on our health care system, it is important to understand how these services affect health promotion outcomes. IMPLICATIONS FOR PRACTICE: The results of this systematic literature review substantiate the need for primary care providers to study MWV efficacy on health promotion outcomes for the Medicare population.

2.
Am J Chin Med ; 48(4): 987-1003, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-32431181

RESUMO

Our previous report revealed that Gardenia jasminoides (GJ) has protective effects against acute pancreatitis. So, we examined whether aqueous extract of GJ has anti-inflammation and antifibrotic effects even against cerulein-induced chronic pancreatitis (CP). CP was induced in mice by an intraperitoneal injection of a stable cholecystokinin (CCK) analogue, cerulein, six times a day, four days per week for three weeks. GJ extract (0.1 or 1[Formula: see text]g/kg) or saline (control group) were intraperitoneally injected 1[Formula: see text]h before first cerulein injection. After three weeks of stimulation, the pancreas was harvested for the examination of several fibrotic parameters. In addition, pancreatic stellate cells (PSCs) were isolated using gradient methods to examine the antifibrogenic effects of GJ. In the cerulein-induced CP mice, the histological features of the pancreas showed severe tissue damage such as enlarged interstitial spaces, inflammatory cell infiltrate and glandular atrophy, and tissue fibrosis. However, treatment of GJ reduced the severity of CP such as pancreatic edema and inflammatory cell infiltration. Furthermore, treatment of GJ increased pancreatic acinar cell survival, and reduced pancreatic fibrosis and activation of PSC in vivo and in vitro. In addition, GJ treatment inhibited the activation of c-Jun N-terminal kinase (JNK) and extracellular signal-regulated protein kinase (ERK) in the PSCs. These results suggest that GJ attenuated the severity of CP and the pancreatic fibrosis by inhibiting JNK and ERK activation during CP.

3.
Environ Sci Pollut Res Int ; 27(16): 20490-20499, 2020 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-32246418

RESUMO

In this study, indirect carbonation was carried out by using cement kiln dust (CKD), an alkaline industrial by-product, and three chelating agents (citrate, malonate, and adipate salts) as solvents at the room temperature and atmospheric pressure. We derived the optimum conditions for eluting Ca from CKD, as well as those for storing CO2 and producing CaCO3 through carbonation. The most important factor affecting the Ca elution from CKD was the solvent concentration and that for the carbonation was the end-of-carbonation pH. Under the optimum conditions of Ca elution, the molar ratios of Ca and solvent in eluates were 1:1, 1:2, and 1:2, respectively, using citrate, malonate, and adipate solvents. Based on the results, we propose that one molecule of Ca ion and one molecule citrate that is tridentate are combined to form a complex. The bidentate malonate and adipate, on the other hand, form complexes by combining one molecule of Ca ion and two molecules of each solvent. It is essential to raise the pH while simultaneously minimizing the amount of free chelating agent in solution to produce more CaCO3 and prevent its dissolution. Besides, it is absolutely necessary to terminate the carbonation reaction at a pH of about 10.5 to improve the reuse efficiency of the chelating agent. CaCO3 produced through carbonation reaction started to dissolve at pH approximately 10.5. All of the CaCO3 produced was calcite with a purity of 98%. The efficiency of Ca elution from CKD using three solvents increased significantly with increasing stability constant of a Ca-ligand complex, but the efficiency of carbonation was the same for all solvents.

4.
Am J Physiol Regul Integr Comp Physiol ; 318(5): R843-R854, 2020 May 01.
Artigo em Inglês | MEDLINE | ID: mdl-32186196

RESUMO

Cisplatin is a well-known chemotherapy medication used to treat numerous cancers. However, treatment with cisplatin in cancer therapy has major side effects, such as nephrotoxic acute kidney injury. Adult vertebrate kidneys are commonly used as models of cisplatin-induced nephrotoxic acute kidney injury. Embryonic zebrafish kidney is more simplified and is composed simply of two nephrons and thus is an excellent model for the investigation of cisplatin nephrotoxicity. Here, we developed a novel model to induce cisplatin nephrotoxicity in adult zebrafish and demonstrated that intraperitoneal injection of cisplatin caused a decline in kidney proximal tubular function based on fluorescein-labeled dextran uptake and alkaline phosphatase staining. We also showed that cisplatin induced histological injury of the kidney tubules, quantified by tubular injury scores on the periodic acid-Schiff-stained kidney sections. As shown in a mouse model of cisplatin-induced nephrotoxicity, the activation of poly(ADP-ribose) polymerase (PARP), an enzyme implicated in cisplatin-induced cell death, was markedly increased after cisplatin injection in adult zebrafish. Furthermore, pharmacological inhibition of PARP using a specific PARP inhibitor PJ 34 hydrochloride (PJ34) or 3-aminobenzamide ameliorated kidney proximal tubular functional and histological damages in cisplatin-injected adult zebrafish kidneys. Administration of a combination of PARP inhibitors PJ34 and 3-aminobenzamide additively protected renal function and histology in zebrafish and mouse models of cisplatin nephrotoxicity. In conclusion, these data suggest that adult zebrafish are not only suitable for drug screening and genetic manipulation but also useful as a simplified but powerful model to study the pathophysiology of cisplatin nephrotoxicity and establish new therapies for treating human kidney diseases.

5.
Nurse Educ ; 2020 Mar 10.
Artigo em Inglês | MEDLINE | ID: mdl-32175953

RESUMO

BACKGROUND: Research suggests flipped learning may improve student motivation to learn. PURPOSE: This study examined motivation and learning strategies among prelicensure BSN students in a course taught using the flipped learning model. The hypothesis was that flipped learning may improve motivation and learning strategies of students. METHODS: A descriptive, comparative design examined motivation and learning strategies used by students in a flipped learning course. The Motivated Strategies for Learning Questionnaire and a demographic survey were administered to 25 BSN students at a Midwestern university. RESULTS: Substantial decreases were found from pretest to posttest mean scores for the task value, control of learning beliefs, and help seeking subscales. CONCLUSION: The results did not support the hypothesis that flipped learning would improve motivation and learning strategies. Further research in larger diverse samples of students in a flipped learning course is recommended.

6.
J Am Coll Health ; : 1-5, 2020 Feb 11.
Artigo em Inglês | MEDLINE | ID: mdl-32045338

RESUMO

Objective: Food insecurity in college students is associated with poor eating habits. The purpose of this project was to increase consumption of fruits and vegetables in college students using a campus food pantry. Participants: Twenty-nine college students completed the study. Methods: Participants viewed weekly food preparation videos demonstrating cost-effective recipes containing fruits and vegetables and a key take-away message. Participants were provided a recipe and food to cook at home based on the Cooking Matters Food Pantry Toolkit. A 24-h dietary recall was collected pre- and postintervention using the Automated Self-Administered 24-H Dietary Assessment. This descriptive comparative study used repeated measure ANOVA. Results: Females (n = 19) increased their fruit and vegetable consumption by 1.3 cups daily (p < .05) while males (n = 10) decreased fruit and vegetable consumption at the postintervention measurement. Conclusion: Increased consumption of fruits and vegetables in female college students using the food pantry was a modifiable behavior in this study.

7.
Medicine (Baltimore) ; 99(7): e19074, 2020 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-32049808

RESUMO

Eight Constitution Medicine (ECM) is a Korean constitutional medicine system that classifies people into 8 types: Pulmotonia (PUL), Colonotonia (COL), Renotonia (REN), Vesicotonia (VES), Pancreotonia (PAN), Gastrotonia (GAS), Hepatonia (HEP), and Cholecystonia (CHO). Metabolic syndrome (MS) is a major public health problem worldwide. We assessed the prevalence of and associations between ECM and MS. Cross-sectional convenience sample of 245 adults was used at a medical check-up center in Seoul, South Korea, from 2010 to 2015. Adults were classified into 1 of 8 constitutions by an ECM specialist. MS was diagnosed on the basis of National Cholesterol Education Program Adult Treatment Panel III and Asian Pacific Criteria for abdominal obesity. We also computed the prevalence by percentage and calculated odds ratios (ORs) for MS among 6 constitutions with PUL as the reference.Among 245 adults, 20 (8.2%) were diagnosed with PUL, 43 (17.6%) with COL, 35(14.3%) with REN, 4 (1.6%) with VES, 71 (29.0%) with PAN, 0 (0.0%) with GAS, 54 (22.0%) with HEP, and 18 (7.3%) with CHO. The prevalence of MS in the constitutions was significantly different: CHO, 38.9%; HEP, 35.2%; PAN, 18.3%; COL, 11.6%; PUL, 5.0%; REN, 2.9% (P = .001). We observed higher ORs for HEP and CHO (OR = 13.03, 95% confidence interval [CI] = 1.61-105.70; and OR = 13.19, 95% CI = 1.39-125.46, respectively) than for the other constitutions.People with HEP and CHO constitutions could be at higher risk for MS. Therefore, ECM-based diagnosis may be useful for preventing and managing MS.


Assuntos
Síndrome Metabólica/classificação , Síndrome Metabólica/epidemiologia , Adulto , Idoso , Estudos Transversais , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Prevalência , República da Coreia/epidemiologia , Medicina Estatal
8.
J Vasc Surg Venous Lymphat Disord ; 8(1): 100-105, 2020 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-31402294

RESUMO

OBJECTIVE: Venous reflux may occur in all parts of the great saphenous vein (GSV). The GSV diameter generally increases when venous reflux occurs, and the extent of venous dilation may be altered on the basis of size and location of the reflux within the GSV. We examined which part of the GSV is the most sensitive and dilated in association with venous reflux. METHODS: We retrospectively evaluated the data of 99 patients (198 limbs) with signs and symptoms of venous insufficiency of both lower limbs from January 2016 to December 2016. We performed ultrasound to examine the venous reflux and to measure the diameter of the GSV. The GSV was divided into four locations: saphenofemoral junction, midthigh, lower thigh (LT), and below the knee. The patients were divided into two groups according to the presence or absence of reflux. RESULTS: There were 87 limbs that had venous reflux and 111 limbs that had no reflux. The diameter of the GSV with reflux was significantly larger than that of GSVs without reflux only at the LT (4.7 mm vs 4.2 mm; P < .001), and the highest area under the receiver operating characteristic curve was 0.642 at the LT. The cutoff value of the LT diameter for association with reflux was 5 mm (P = .025). CONCLUSIONS: The cutoff diameter of the LT was 5 mm. We recommend treatment of symptomatic reflux, and LT diameter may be useful for follow-up before and after treatment.

9.
Mol Med Rep ; 21(1): 258-266, 2020 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-31746359

RESUMO

The major role of inner medullary collecting duct (IMCD) cells is to maintain water and sodium homeostasis. In addition to the major role, it also participates in the protection of renal and systemic inflammation. Although IMCD cells could take part in renal and systemic inflammation, investigations on renal inflammation in IMCD cells have rarely been reported. Although berberine (BBR) has been reported to show diverse pharmacological effects, its anti­inflammatory and protective effects on IMCD cells have not been studied. Therefore, in the present study, we examined the anti­inflammatory and protective effects of BBR in mouse IMCD­3 (mIMCD­3) cells against lipopolysaccharide (LPS). An MTT assay was carried out to investigate the toxicity of BBR on mIMCD­3 cells. Reverse transcription quantitative­PCR and western blotting were performed to analysis pro­inflammatory molecules and cytokines. Mechanisms of BBR were examined by western blotting and immunocytochemistry. According to previous studies, pro­inflammatory molecules, such as inducible nitric oxide synthase and cyclooxygenase­2, and pro­inflammatory cytokines, such as interleukin (IL)­1ß, IL­6 and tumor necrosis factor­α are increased in LPS­exposed mIMCD­3 cells. However, the production of these pro­inflammatory molecules is significantly inhibited by treatment with BBR. In addition, BBR inhibited translocation of nuclear factor (NF)­κB p65 from the cytosol to the nucleus, and degradation of inhibitory κ­Bα in LPS­exposed mIMCD­3 cells. In conclusion, BBR could inhibit renal inflammatory responses via inhibition of NF­κB signaling and ultimately contribute to amelioration of renal injury during systemic inflammation.

10.
Fish Shellfish Immunol ; 96: 279-289, 2020 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-31783148

RESUMO

The interferon-induced GTP-binding protein Mx is responsible for a specific antiviral state against a broad spectrum of viral infections that are induced by type-I interferons (IFN α/ß) in different vertebrates. In this study, the Mx gene was isolated from the constructed mullet cDNA database. Structural features of mullet Mx (MuMx) were analyzed using different in-silico tools. The pairwise comparison revealed that the MuMx sequence was related to Stegastes partitus Mx with an 83.7% sequence identity, whereas MuMx was clustered into the teleost category in the phylogentic analysis. Sequence alignment showed that the dynamin-type guanine nucleotide-binding domain (G_DYNAMIN_2), central interactive domain (CID), and GTPase effector domain (GED) were conserved among Mx counterparts. The transcriptional expression of MuMx was the highest in blood cells from unchallenged fish. The temporal mRNA profile showed that MuMx expression was significantly elevated in all tissues, including blood, spleen, head kidney, liver, and gills after the injection of polyinosinic-polycytidylic acid (poly I:C) at many time points. Moreover, MuMx expression increased slightly, in the blood, spleen, and head kidney at a few time points after the injection of lipopolysaccharide (LPS) and Lactococcus garvieae (L. garvieae). Results of the subcellular localization analysis confirmed that the MuMx protein was highly expressed in the cytoplasm. The analysis of the gene expression of the viral hemorrhagic septicemia virus (VHSV) under conditions of MuMx overexpression confirmed the significant inhibition of viral transcripts. The cell viability (MTT) assay and VHSV titer quantification with the presence of MuMx indicated a significant reduction in virus replication. Collectively, these findings suggest that Mx is a specific immune-related gene that elicits crucial antiviral functions against viral antigens in the mullet fish.


Assuntos
Doenças dos Peixes/imunologia , Regulação da Expressão Gênica/imunologia , Imunidade Inata/genética , Proteínas de Resistência a Myxovirus/genética , Proteínas de Resistência a Myxovirus/imunologia , Smegmamorpha/genética , Smegmamorpha/imunologia , Sequência de Aminoácidos , Animais , Proteínas de Peixes/química , Proteínas de Peixes/genética , Proteínas de Peixes/imunologia , Expressão Gênica , Perfilação da Expressão Gênica/veterinária , Infecções por Bactérias Gram-Positivas/imunologia , Infecções por Bactérias Gram-Positivas/veterinária , Lactococcus/fisiologia , Lipopolissacarídeos/farmacologia , Proteínas de Resistência a Myxovirus/química , Novirhabdovirus/fisiologia , Filogenia , Poli I-C/farmacologia , Infecções por Rhabdoviridae/imunologia , Infecções por Rhabdoviridae/veterinária , Alinhamento de Sequência/veterinária
11.
Pancreas ; 49(1): 89-95, 2020 01.
Artigo em Inglês | MEDLINE | ID: mdl-31856083

RESUMO

OBJECTIVES: In this study, we investigated the anti-inflammatory effects of silymarin on cerulein-induced acute pancreatitis (AP) in mice. METHODS: Cerulein (50 µg/kg) was injected intraperitoneally once hourly for 6 hours to induce AP. To investigate the prophylactic effects of silymarin, dimethyl sulfoxide or silymarin (25, 50, and 100 mg/kg) was injected intraperitoneally 1 hour before cerulein injection. To investigate the therapeutic effects of silymarin, dimethyl sulfoxide or silymarin (100 mg/kg) was injected intraperitoneally 1, 3, or 5 hours after the first cerulein injection. Blood, pancreas, and lungs were harvested 6 hours after the last cerulein injection. RESULTS: Pre- and posttreatment with silymarin decreased the pancreas weight/body weight ratio and serum amylase activity. Furthermore, silymarin treatment inhibited pancreas and lung injury and neutrophil infiltration during cerulein-induced AP. In addition, silymarin inhibited increased secretion of proinflammatory cytokines such as interleukin 1ß, interleukin 6, and tumor necrosis factor α. Finally, mitogen-activated protein kinases (MAPKs) and nuclear factor-κB were activated by cerulein, and only p38 in MAPK was inhibited by silymarin. CONCLUSIONS: These findings suggest that silymarin attenuates the severity of AP through inhibition of p38 MAPKs and that silymarin could be a potential prophylactic and therapeutic agent for the treatment of AP.

12.
Environ Monit Assess ; 192(1): 41, 2019 Dec 13.
Artigo em Inglês | MEDLINE | ID: mdl-31834560

RESUMO

To evaluate the pretreatment processing for Cd isotope analysis of environmental samples, acid extractions and total digestions were examined with various environmental certified reference materials. Four certified reference material samples, including fly ash, polluted soil, domestic sludge, and industrial sludge, were digested by six different acid extraction and total digestion methods, and then Cd was separated to remove other matrix by anion-exchange column. The Cd recovery rates of the acid extraction methods were 2.6-89.1%, while those of the total digestion methods were 21.6-88.7%. In these results, the Cd recovery rates were dependent on the sample type. More than 80% of the Cd in the polluted soil and domestic sludge samples could be recovered regardless of the decomposition method, except one method. On the other hand, the Cd recovery rate from fly ash was low when total digestion was performed using a HF mixture, and the recovery rate by total digestion methods for industrial sludge was higher than that by acid extraction. In our results, Cd isotope ratios tended to be more positive by increasing the Cd recovery rates, suggesting that the light isotope of Cd was decomposed preferentially during the decomposition procedures. However, when more than 80% of the Cd in the samples was recovered, the Cd isotope ratios were determined to be similar. This indicated that at least 80% of the Cd should be recovered from environmental samples to accurately measure the Cd isotopic ratio of environmental samples.


Assuntos
Cádmio/análise , Monitoramento Ambiental , Poluentes Ambientais/análise , Ácidos , Cinza de Carvão , Isótopos , Esgotos
13.
Am J Mens Health ; 13(5): 1557988319871423, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31552775

RESUMO

Finasteride is commonly used for treatment of alopecia. Because finasteride is a cause of gynecomastia, there is concern regarding the continuation of finasteride therapy after mastectomy. No studies have been performed to determine whether finasteride should be continued after mastectomy when gynecomastia occurs in patients taking finasteride for the treatment of alopecia. The researchers studied the effects of finasteride on gynecomastia recurrence after mastectomy in men with gynecomastia taking finasteride for alopecia. The researchers retrospectively evaluated 1,673 patients with gynecomastia who underwent subcutaneous mastectomy with liposuction at Damsoyu Hospital from January 2014 to December 2016. In total, 52 of the patients were taking finasteride for alopecia before surgery and continued to use it in the same manner after mastectomy. Ultrasonography was performed 1 year after mastectomy. The patients' median age was 26.5 (24.75-30) years. All 52 patients had bilateral gynecomastia. The median duration of finasteride therapy before and after surgery was 12 (5-25.75) and 33 (27.5-40.5) months, respectively. There were no statistically significant differences between the groups with and without the use of finasteride in relation to postoperative complications and recurrence rates. Taking finasteride seems to have little effect on recurrence in patients with alopecia who have undergone surgical treatment of gynecomastia. Surgeons may recommend continuous finasteride therapy in patients with alopecia who wish to take finasteride after mastectomy.

14.
Mol Med Rep ; 20(4): 3709-3718, 2019 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-31485676

RESUMO

Chronic pancreatitis (CP) is characterized by recurrent pancreatic injury, resulting in inflammation and fibrosis. Currently, there are no drugs for the treatment of pancreatic fibrosis associated with CP. Piperine, a natural alkaloid found in black pepper, has been reported to show anti­inflammatory, anti­oxidative, and antitumor activities. Although piperine exhibits numerous properties in regards to the regulation of diverse diseases, the effects of piperine on CP have not been established. To investigate the effects of piperine on CP in vivo, we induced CP in mice through the repetitive administration of cerulein (50 µg/kg) six times at 1­h intervals, 5 times per week, for a total of 3 weeks. In the pre­treatment groups, piperine (1, 5, or 10 mg/kg) or corn oil were administrated orally at 1 h before the first cerulein injection, once a day, 5 times a week, for a total of 3 weeks. In the post­treatment groups, piperine (10 mg/kg) or corn oil was administered orally at 1 or 2 week after the first cerulein injection. Pancreases were collected for histological analysis. In addition, pancreatic stellate cells (PSCs) were isolated to examine the anti­fibrogenic effects and regulatory mechanisms of piperine. Piperine treatment significantly inhibited histological damage in the pancreas, increased the pancreatic acinar cell survival, reduced collagen deposition and reduced pro­inflammatory cytokines and chemokines. In addition, piperine treatment reduced the expression of fibrotic mediators, such as α­smooth muscle actin (α­SMA), collagen, and fibronectin 1 in the pancreas and PSCs. Moreover, piperine treatment reduced the production of transforming growth factor (TGF)­ß in the pancreas and PSCs. Furthermore, piperine treatment inhibited TGF­ß­induced pSMAD2/3 activation but not pSMAD1/5 in the PSCs. These findings suggest that piperine treatment ameliorates pancreatic fibrosis by inhibiting TGF­ß/SMAD2/3 signaling during CP.


Assuntos
Alcaloides/uso terapêutico , Anti-Inflamatórios/uso terapêutico , Benzodioxóis/uso terapêutico , Pancreatite Crônica/tratamento farmacológico , Piperidinas/uso terapêutico , Alcamidas Poli-Insaturadas/uso terapêutico , Proteínas Smad/imunologia , Fator de Crescimento Transformador beta/imunologia , Animais , Modelos Animais de Doenças , Feminino , Fibrose , Camundongos , Camundongos Endogâmicos C57BL , Pâncreas/efeitos dos fármacos , Pâncreas/imunologia , Pâncreas/patologia , Pancreatite Crônica/imunologia , Pancreatite Crônica/patologia , Transdução de Sinais/efeitos dos fármacos
15.
Fish Shellfish Immunol ; 93: 597-611, 2019 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-31400511

RESUMO

The transcription factor, activator protein-1 (AP-1), is a dimeric protein and a downstream member of the mitogen-activated protein kinase (MAPK) signaling pathway. It regulates a wide array of functions including, cell proliferation, survival, differentiation, response to UV-irradiation, immune responses, and inflammatory conditions. AP-1 belongs to the basic leucine zipper (bZIP) protein family, which consists of members from Jun, Fos, Maf, and ATF subfamilies. In the present study, c-Jun and c-Fos homologs were identified from a transcriptome database of Liza haematocheila and designated as Lhc-Jun and Lhc-Fos. In both sequences, the signature bZIP domain was identified and also the DNA binding sites, dimerization sites, as well as the phosphorylation sites, were found to be highly conserved through evolution. Tissue distribution analysis revealed that both Lhc-Jun and Lhc-Fos transcripts were ubiquitously expressed in all examined tissues of healthy mullets. In order to determine the transcriptional modulations of Lhc-Jun and Lhc-Fos, challenge experiments were carried out using LPS, poly I:C, and L. garvieae. The qRT-PCR analysis revealed significant upregulation of Lhc-Jun and Lhc-Fos in blood, gill, liver, and spleen. This is the first study that explores the correlation between UV-irradiation and AP-1 ortholog expression in teleosts. Also, this is the first time that the functional characterization of the teleost c-Fos ortholog has been carried out. Sub-cellular localization of Lhc-Jun and Lhc-Fos was observed in the nucleus. AP-1-Luc reporter assays revealed significant higher luciferase activities in both Lhc-Jun and Lhc-Fos proteins compared to mock controls. These results strongly suggest that Lhc-Jun and Lhc-Fos might play a significant role in Liza haematocheila immunity by regulating AP-1 promoter sequences in immune and stress-related genes.


Assuntos
Doenças dos Peixes/imunologia , Peixes/genética , Peixes/imunologia , Regulação da Expressão Gênica/imunologia , Imunidade Inata/genética , Fator de Transcrição AP-1/genética , Fator de Transcrição AP-1/imunologia , Sequência de Aminoácidos , Animais , Proteínas de Peixes/química , Proteínas de Peixes/genética , Proteínas de Peixes/imunologia , Perfilação da Expressão Gênica/veterinária , Infecções por Bactérias Gram-Positivas/imunologia , Infecções por Bactérias Gram-Positivas/veterinária , Lactococcus/fisiologia , Lipopolissacarídeos/farmacologia , Filogenia , Poli I-C/farmacologia , Proteínas Proto-Oncogênicas c-fos/genética , Proteínas Proto-Oncogênicas c-fos/imunologia , Proteínas Proto-Oncogênicas c-jun/genética , Proteínas Proto-Oncogênicas c-jun/imunologia , Alinhamento de Sequência/veterinária , Fator de Transcrição AP-1/química
16.
Int J Mol Med ; 44(4): 1563-1573, 2019 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-31432106

RESUMO

Acute pancreatitis (AP) is an inflammatory disease of the pancreas. Icariin (ICA), a flavonoid glycoside, has been reported to have several pharmacological effects; however, the anti­inflammatory effects of ICA against AP require further study. Therefore, we aimed to investigate the effect of ICA on cerulein­induced AP. In the present study, AP was induced by intraperitoneally administering a supramaximal concentration of cerulein (50 µg/kg/h) for 6 h. ICA was also administered intraperitoneally, and mice were sacrificed 6 h after the final cerulein injection. Blood samples were collected to determine serum amylase and lipase levels. The pancreas and lung were rapidly removed for histological examination, and the analysis of myeloperoxidase activity. In addition, reverse transcription­quantitative polymerase chain reaction was conducted to analyze the expression of inflammatory cytokines in pancreatic tissues. Our results revealed that the administration of ICA prevented an increase in the pancreas weight/body weight ratio of mice and serum digestive enzyme levels. ICA treatment also inhibited cerulein­induced histological injury and neutrophil infiltration of the pancreas and lung. In addition, ICA suppressed the production of pro­inflammatory cytokines, including interleukin (IL)­1ß, IL­6 and tumor necrosis factor­α in the pancreas. Furthermore, ICA administration was observed to inhibit p38 activation during cerulein­induced AP. Inhibition of p38 activation resulted in alleviated pancreatitis. Collectively, our results suggested that ICA exhibits anti­inflammatory effects in cerulein­induced AP via the inhibition of p38.


Assuntos
Ceruletídeo/efeitos adversos , Flavonoides/farmacologia , Pancreatite/etiologia , Pancreatite/metabolismo , Proteínas Quinases p38 Ativadas por Mitógeno/antagonistas & inibidores , Proteínas Quinases p38 Ativadas por Mitógeno/metabolismo , Amilases/sangue , Animais , Biomarcadores , Modelos Animais de Doenças , Feminino , Lipase/sangue , Lipase/metabolismo , Camundongos , NF-kappa B/metabolismo , Pancreatite/diagnóstico , Pancreatite/tratamento farmacológico , Índice de Gravidade de Doença
18.
J Surg Case Rep ; 2019(8): rjz242, 2019 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-31462985

RESUMO

Angioleiomyoma and lipoleiomyoma are rarely found tumors in the inguinal canal. We present two cases of laparoscopic removal of angioleiomyoma and lipoleiomyoma in the female inguinal canal. In Case 1, a 35-year-old woman was admitted to our hospital with left inguinal swelling and intermittent pain. A hypoechoic well-marginated mass was observed on ultrasonography. Laparoscopic complete tumor excision and posterior wall repair were performed, and a diagnosis of angioleiomyoma was confirmed on histopathological evaluation. In Case 2, a 55-year-old woman was admitted with a painful right inguinal swelling. A hypoechoic well-marginated ovoid mass was observed on ultrasonography. Laparoscopic complete tumor excision and posterior wall repair were performed, and a diagnosis of lipoleiomyoma was confirmed on histopathological evaluation. Laparoscopic tumor excision and posterior wall repair are effective treatments of leiomyomas in the inguinal canal.

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