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1.
PLoS One ; 14(7): e0218216, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31269030

RESUMO

PURPOSE: To determine the influence of residual submacular fluid (SMF) on the recovery of function and structure of the retina after successful rhegmatogenous retinal detachment (RRD) reattachment. METHODS: We reviewed the medical records of all patients who had undergone successful RRD repair by scleral buckling (SB) surgery or by pars plana vitrectomy (PPV) from March 2011 to August 2014. Spectral-domain optical coherence tomographic images of the macular regions were used at 1, 2, 3, 6, 9, and 12 months following the surgery. The best-corrected visual acuities (BCVA) were evaluated at the same times. RESULTS: The eyes with a macula-off RRD that were treated by SB surgery had a significant higher incidence of residual SMF (52%) than those treated by PPV (6.8%; P <0.001). Nevertheless, the postoperative BCVA was significantly improved in the eyes that had undergone SB surgery (P = 0.007). The postoperative BCVAs were not significantly different between the groups in which the SMF was absorbed (12 eyes) and not absorbed (13 eyes) within 1 month after the SB surgery. The photoreceptor outer segment length and the presence of a foveal bulge were not significantly different between these two groups at 12 months. Multiple regression analyses showed that the presence of a foveal bulge (ß = 0.531, P = 0.001) and the duration of the retinal detachment before surgery (ß = 0.465, P = 0.002) but not the duration of the SMF were independent factors significantly correlated with the final BCVA. CONCLUSIONS: These results suggest that the postoperative residual SMF does not significantly disrupt the functional and structural recovery of eyes with macula-off RRD treated by SB surgery.

2.
J Nutr Biochem ; 70: 116-124, 2019 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-31200316

RESUMO

We have previously shown that ascorbic acid (AsA) deficiency elevates hepatic expression of acute phase proteins (APPs), inflammatory markers, in Osteogenic Disorder Shionogi (ODS) rats, which are unable to synthesize AsA. However, the precise mechanisms of this elevation are unknown. Signal transducer and activator of transcription 3 (STAT3) is one of the transcription factors inducing the expression of APPs and is activated by several cytokines including interleukin-6 (IL-6). The aim of this study was to determine whether AsA deficiency stimulates hepatic STAT3 activation and increases intestinal production of proinflammatory cytokines such as IL-6. Male ODS rats (6 weeks old) were fed either a basal diet containing 300 mg AsA/kg (control group) or an AsA-free diet (AsA-deficient group) for 18 days. AsA deficiency gradually and simultaneously elevated both mRNA levels of APPs (haptoglobin, α1-acid glycoprotein, C-reactive protein and α2-macroglobulin) and nuclear level of phosphorylated STAT3 (activated STAT3) in the liver. These results showed that the AsA-deficiency-induced expression of hepatic APPs is stimulated by proinflammatory cytokines activating STAT3. On day 14, AsA deficiency significantly elevated IL-6 mRNA level in the ileum and the concentration of IL-6 in portal blood. Furthermore, the portal concentration of IL-6 positively correlated with hepatic mRNA levels of STAT3-regulated genes. These findings suggest that IL-6, produced in the intestine as a result of AsA deficiency, is recruited to the liver via the portal vein and contributes to hepatic STAT3 activation and the elevated expression of APPs.

3.
Intern Med ; 58(14): 2101-2105, 2019 Jul 15.
Artigo em Inglês | MEDLINE | ID: mdl-30918176

RESUMO

A 43-year-old man with malignant lymphoma who had been treated with the cyclosphamide, vincrstine, procarbazine, and prednisolone (C-MOPP) regimen was admitted to our hospital with skin eruption. He was diagnosed to have varicella, and treatment with acyclovir and immune globulin was started. Chest computed tomography revealed multiple nodules in the both lung fields. Diagnostic thoracoscopic lung biopsy specimens revealed granuloma formation, and polymerase chain reaction testing revealed the presence of varicella-zoster virus DNA in the granulomatous tissue. It was unusual for the lung nodule in varicella pneumonia to increase in size over time in a patient who had undergone antiviral therapy, while also demonstrating multiple granulomas.

4.
Sci Rep ; 9(1): 3217, 2019 Mar 01.
Artigo em Inglês | MEDLINE | ID: mdl-30824755

RESUMO

We evaluated the preoperative optical coherence tomographic (OCT) findings in eyes with macula-off rhegmatogenous retinal detachment (RRD) and determined the factors that were significantly correlated with the postoperative best-corrected visual acuity (BCVA). The length of the preoperative photoreceptors was defined as the distance between the external limiting membrane (ELM) and the outer end of the outer segments of the photoreceptors in the OCT images. The mean length of the photoreceptors was 102.8 ± 28.7 µm with a range of 20 to 159 µm in eyes with RRD. The length of the preoperative photoreceptors was not significantly correlated with the preoperative BCVA but it was significantly correlated with the postoperative BCVA (r = -0.353, P = 0.003). Multivariate regression analyses revealed that the length of the photoreceptors (ß = -0.388, P = 0.001) and the preoperative BCVA (ß = 0.274, P = 0.021) were the only independent factors that were significantly associated with the postoperative BCVA. The length of the preoperative photoreceptors was significantly correlated with the postoperative photoreceptor length (r = 0.486, P < 0.001). Longer preoperative photoreceptors were significantly correlated with longer postoperative photoreceptors and better BCVA after successful reattachment. These results suggest that the preoperative length of the photoreceptors can be good factor to use for predicting the final BCVA following successful reattachment of macula-off RRD.

5.
Mamm Genome ; 29(3-4): 273-280, 2018 04.
Artigo em Inglês | MEDLINE | ID: mdl-29523950

RESUMO

Streptozotocin (STZ) has been widely used to induce diabetes in rodents. Strain-dependent variation in susceptibility to STZ has been reported; however, the gene(s) responsible for STZ susceptibility has not been identified. Here, we utilized the A/J-11SM consomic strain and a set of chromosome 11 (Chr. 11) congenic strains developed from A/J-11SM to identify a candidate STZ-induced diabetes susceptibility gene. The A/J strain exhibited significantly higher susceptibility to STZ-induced diabetes than the A/J-11SM strain, confirming the existence of a susceptibility locus on Chr. 11. We named this locus Stzds1 (STZ-induced diabetes susceptibility 1). Congenic mapping using the Chr. 11 congenic strains indicated that the Stzds1 locus was located between D11Mit163 (27.72 Mb) and D11Mit51 (36.39 Mb). The Mpg gene, which encodes N-methylpurine DNA glycosylase (MPG), a ubiquitous DNA repair enzyme responsible for the removal of alkylated base lesions in DNA, is located within the Stzds1 region. There is a close relationship between DNA alkylation at an early stage of STZ action and the function of MPG. A Sanger sequence analysis of the Mpg gene revealed five polymorphic sites in the A/J genome. One variant, p.Ala132Ser, was located in a highly conserved region among rodent species and in the minimal region for retained enzyme activity of MPG. It is likely that structural alteration of MPG caused by the p.Ala132Ser mutation elicits increased recognition and excision of alkylated base lesions in DNA by STZ.


Assuntos
Mapeamento Cromossômico/métodos , Cromossomos de Mamíferos/genética , Diabetes Mellitus Experimental/genética , Estudos de Associação Genética , Predisposição Genética para Doença , Animais , Glicemia/metabolismo , Diabetes Mellitus Experimental/sangue , Feminino , Loci Gênicos , Insulina/sangue , Masculino , Camundongos Congênicos , Estreptozocina , Fatores de Tempo
6.
J Nutr Sci Vitaminol (Tokyo) ; 64(6): 404-411, 2018.
Artigo em Inglês | MEDLINE | ID: mdl-30606963

RESUMO

The aim of this study was to verify the protective effects of ascorbic acid (AsA) against lipopolysaccharide (LPS)-induced sepsis. The study was conducted using osteogenic disorder Shionogi (ODS) rats, which are unable to synthesize AsA. Male ODS rats (6 wk old) were fed either an AsA-free diet (AsA-deficient group), a diet supplemented with 300 mg/kg AsA (control group), or a diet supplemented with 3,000 mg/kg AsA (high-AsA group) for 8 d. On day 8, all the rats were intraperitoneally injected with LPS (15 mg/kg body weight). Forty-eight hours after the injection, the survival rates of the rats in the control (39%) and the high-AsA (61%) groups were significantly higher than that in the AsA-deficient group (5.5%). Next, we measured several inflammatory parameters during 10 h after administering LPS. At 6 h, elevated serum levels of markers for hepatic and systemic injuries were suppressed in rats fed AsA. Similarly, 10 h after LPS injection, the elevation in the serum levels of markers for renal injury were also suppressed proportionally to the amount of AsA in the diet. The elevated serum concentrations of TNFα and IL-1ß by LPS in the AsA-deficient group decreased in groups fed AsA. Hematic TNFα mRNA levels at 6 h after the LPS injection were also lowered by feeding AsA. These results demonstrated that the dietary intake of AsA improved the survival rates and suppressed the inflammatory damage, in a dose-dependent manner, caused during sepsis induced by LPS in ODS rats.


Assuntos
Ácido Ascórbico/uso terapêutico , Suplementos Nutricionais , Inflamação/prevenção & controle , Rim/efeitos dos fármacos , Fígado/efeitos dos fármacos , Sepse/tratamento farmacológico , Vitaminas/uso terapêutico , Animais , Anti-Inflamatórios/administração & dosagem , Anti-Inflamatórios/sangue , Anti-Inflamatórios/farmacologia , Anti-Inflamatórios/uso terapêutico , Ácido Ascórbico/administração & dosagem , Ácido Ascórbico/sangue , Ácido Ascórbico/farmacologia , Deficiência de Ácido Ascórbico/sangue , Deficiência de Ácido Ascórbico/complicações , Deficiência de Ácido Ascórbico/genética , Deficiência de Ácido Ascórbico/prevenção & controle , Dieta , Inflamação/induzido quimicamente , Interleucina-1beta/sangue , Nefropatias/sangue , Nefropatias/etiologia , Nefropatias/prevenção & controle , Lipopolissacarídeos , Hepatopatias/sangue , Hepatopatias/etiologia , Hepatopatias/prevenção & controle , Masculino , Estado Nutricional , Osteogênese/fisiologia , Ratos , Ratos Endogâmicos , Sepse/induzido quimicamente , Sepse/complicações , Fator de Necrose Tumoral alfa/sangue , Vitaminas/administração & dosagem , Vitaminas/sangue , Vitaminas/farmacologia
7.
PLoS One ; 12(9): e0184783, 2017.
Artigo em Inglês | MEDLINE | ID: mdl-28902881

RESUMO

PURPOSE: To determine the perioperative factors that are significantly correlated with the final visual acuity following reattachment of a macula-off rhegmatogenous retinal detachment (RRD) by vitrectomy. METHODS: Twenty-nine eyes of 29 patients with a successfully reattached RRD by vitrectomy were retrospectively analyzed. Spectral-domain optical coherence tomographic images of the macular regions were used to measure the thicknesses of the retinal layers and the integrity of the microstructures of the photoreceptors at 2 weeks, 1, 2, 3, 6, 9, and 12 months following the vitrectomy. The best-corrected visual acuities (BCVA) were evaluated at the same times. RESULTS: The improvement of the BCVA from the preoperative BCVA to that at postoperative Week 2 (-0.67 ± 0.69 logMAR units) was the largest change between adjacent observation periods for the entire study duration. It was significantly greater than the improvement between Week 2 and Month 12 (-0.32 ± 0.22 logMAR units; P<0.001). The thickness of the ellipsoid zone (EZ)-retinal pigment epithelium (RPE) increased significantly with time (P<0.001). The final BCVA was significantly correlated with the BCVA at Week 2 (r = 0.61, P<0.001), the EZ-RPE thickness at Week 2 (r = -0.40, P = 0.035), the integrity of the external limiting membrane (ELM) (r = -0.61, P = 0.003), and an intact EZ (r = -0.66, P = 0.001) at Week 2. Multiple stepwise regression analyses of the final BCVA showed that the BCVA at Week 2 (P = 0.017) and the integrity of the EZ at Week 2 (P = 0.006) were independent predictors of the final BCVA. CONCLUSIONS: The significantly better BCVA and presence of an intact EZ at 2 weeks following vitrectomy and their significant correlations with the BCVA at Month 12 indicate that these perioperative values can be used to predict the BCVA at Month 12 after a reattachment of macula-off RRD following vitrectomy.


Assuntos
Retina/cirurgia , Descolamento Retiniano/cirurgia , Acuidade Visual , Idoso , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Período Pós-Operatório , Retina/diagnóstico por imagem , Estudos Retrospectivos
8.
J Nutr Sci Vitaminol (Tokyo) ; 63(3): 200-207, 2017.
Artigo em Inglês | MEDLINE | ID: mdl-28757535

RESUMO

Coffee consumption reduces the risk of type 2 diabetes in humans, but the mechanism remains unclear. In this study, we investigated the effect of coffee on pancreatic ß-cells in the induction of diabetes by streptozotocin (STZ) treatment in mice. We examined the effect of coffee, caffeine, or decaffeinated coffee ingestion on STZ-induced hyperglycemia. After STZ injection in Exp. 1 and 2, serum glucose concentration and water intake in coffee ingestion (Coffee group) tended to be lowered or was significantly lowered compared to those in water ingestion (Water group) instead of coffee. In Exp. 1, the values for water intake and serum glucose concentration in caffeine ingestion (Caffeine group) were similar to those in the Water group. In Exp. 2, serum glucose concentrations in the decaffeinated coffee ingestion (Decaf group) tended to be lower than those in the Water group. Pancreatic insulin contents tended to be higher in the Coffee and Decaf groups than in the Water group (Exp. 1 and 2). In Exp. 3, subsequently, we showed that coffee ingestion also suppressed the deterioration of hyperglycemia in diabetic mice which had been already injected with STZ. This study showed that coffee ingestion prevented the development of STZ-induced diabetes and suppressed hyperglycemia in STZ-diabetic mice. Caffeine or decaffeinated coffee ingestion did not significantly suppress STZ-induced hyperglycemia. These results suggest that the combination of caffeine and other components of decaffeinated coffee are needed for the preventive effect on pancreatic ß-cell destruction. Coffee ingestion may contribute to the maintenance of pancreatic insulin contents.


Assuntos
Café , Diabetes Mellitus Experimental/prevenção & controle , Hiperglicemia/sangue , Animais , Glicemia/metabolismo , Cafeína/farmacologia , Hiperglicemia/prevenção & controle , Insulina/sangue , Células Secretoras de Insulina/efeitos dos fármacos , Masculino , Camundongos , Camundongos Endogâmicos C57BL , Pâncreas/efeitos dos fármacos
9.
Sci Rep ; 7(1): 5997, 2017 Jul 20.
Artigo em Inglês | MEDLINE | ID: mdl-28729551

RESUMO

Although scleral buckling is a well-established surgical treatment for rhegmatogenous retinal detachment (RRD), the procedure can reportedly cause problems in the ocular circulation. Segmental scleral bucking without a concomitant encircling procedure was performed on 46 eyes with successfully reattached macula-on RRD. Choroidal blood flow was assessed using laser speckle flowgraphy. Spectral-domain optical coherence tomography was used to image macular regions, to measure the subfoveal choroidal thickness (SFCT), and to calculate the luminal and the stromal areas by the binarization method preoperatively and 1, 4, 8 and 12 weeks postoperatively. Choroidal mean blur rate at the macula did not significantly change, while that at the buckle and unbuckle side significantly reduced at 8 weeks postoperatively in the operated eye (P = 0.007 and P = 0.017, respectively). The SFCT and the luminal area increased temporarily 1 week following surgery in the operated eye (P < 0.001). The trend of SFCT with time coincided with that of the luminal area (P < 0.001). Venous drainage obstruction induced by compression force of scleral buckling leads to SFCT thickening in the acute postoperative phase. The macular choroidal blood flow might be less susceptible because the blood flow at the macula, in contrast to the other areas, does not change following segmental scleral buckling.

10.
Exp Anim ; 66(3): 245-250, 2017 Aug 05.
Artigo em Inglês | MEDLINE | ID: mdl-28381738

RESUMO

Mice with dominant white spotting occurred spontaneously in the C3.NSY-(D11Mit74-D11Mit229) strain. Linkage analysis indicated that the locus for white spotting was located in the vicinity of the Pax3 gene on chromosome 1. Crosses of white-spotted mice showed that homozygosity for the mutation caused tail and limb abnormalities and embryonic lethality as a result of exencephaly; these phenotypes were analogous to those found in other Pax3 mutants. Sequence analysis identified a missense point mutation (c.101G>A) in exon 2 of Pax3 that resulted in a methionine to isoleucine conversion at amino acid 62 of the PAX3 protein. This mutation site was located in the N-terminal HTH (helix-turn-helix) motif of the paired domain of Pax3, which is necessary for binding to DNA and is highly conserved in vertebrate species. Alteration of DNA binding affinity was responsible for embryonic lethality in homozygotes and white spotting in heterozygotes. We named the mutant allele as Pax3Sp-Nag. The C3H/HeN-Pax3Sp-Nag strain may be useful for analyzing the function of Pax3 as a new model of the human disease, Waardenburg Syndrome.


Assuntos
Mutação de Sentido Incorreto , Fator de Transcrição PAX3/genética , Mutação Puntual , Domínios Proteicos/genética , Síndrome de Waardenburg/genética , Alelos , Sequência de Aminoácidos/genética , Animais , DNA/metabolismo , Modelos Animais de Doenças , Sequências Hélice-Volta-Hélice/genética , Humanos , Isoleucina , Metionina , Camundongos Endogâmicos , Fator de Transcrição PAX3/química , Fator de Transcrição PAX3/metabolismo , Fator de Transcrição PAX3/fisiologia , Ligação Proteica
11.
PLoS One ; 12(3): e0174427, 2017.
Artigo em Inglês | MEDLINE | ID: mdl-28355247

RESUMO

PURPOSE: To investigate ocular blood flow and correlations between ocular blood flow and variables in patients with severe non-proliferative diabetic retinopathy (S-NPDR) following panretinal photocoagulation (PRP). METHODS: In this retrospective, cross-sectional study, the blood flow on the optic nerve head (ONH) and choroid was assessed with laser speckle flowgraphy (LSFG) using the mean blur rate (MBR) in 76 eyes of 76 patients with S-NPDR who underwent PRP, 39 eyes of 39 patients with S-NPDR who did not undergo PRP, and 71 eyes of 71 normal subjects. The correlation between MBR and variables, including visual acuity (VA) and choroidal area determined by binarization method, was analyzed. RESULTS: The mean age was 62.9 ± 11.9 years in the S-NPDR with PRP eyes, 55.6 ± 11.4 years in the S-NPDR without PRP eyes, and 60.3 ± 11.1 years in the normal subject eyes. The ONH MBR in vessel and tissue areas and the choroidal MBR were significantly lower in the S-NDR with PRP group than in the other groups (p < 0.001, p < 0.001, and p < 0.001, respectively). The luminal and the stromal areas were significantly smaller in the S-NDR with PRP group than in the other groups (p < 0.001 and p < 0.001, respectively). LogMAR best corrected visual acuity (BCVA) exhibited significant negative correlation with the ONH MBR in vessel (r = -0.386, p < 0.001), tissue (r = -0.348, p < 0.001), and the choroid MBR (r = -0.339, p = 0.002) in the S-NDR with PRP group. Stepwise multiple regression analysis demonstrated that BCVA was a common independent factor associated with the ONH MBR in vessel, tissue, and the choroidal MBR in the S-NDR with PRP group. CONCLUSIONS: ONH and choroid MBR in addition to choroidal component, including the luminal area, were significantly lower in eyes of patients with S-NPDR after PRP compared with no PRP and normal subjects group. This could suggest that the significantly reduced ocular blood flow in PRP-treated S-NPDR eyes correlated with long-term decreased post-PRP luminal area and visual acuity.


Assuntos
Diabetes Mellitus Tipo 2/complicações , Retinopatia Diabética/terapia , Fluxo Sanguíneo Regional/efeitos da radiação , Adulto , Idoso , Velocidade do Fluxo Sanguíneo , Estudos de Casos e Controles , Corioide/irrigação sanguínea , Corioide/patologia , Estudos Transversais , Feminino , Humanos , Fotocoagulação a Laser , Masculino , Pessoa de Meia-Idade , Disco Óptico/irrigação sanguínea , Estudos Retrospectivos , Resultado do Tratamento
12.
BMC Genet ; 17(1): 145, 2016 11 17.
Artigo em Inglês | MEDLINE | ID: mdl-27855657

RESUMO

BACKGROUND: Nonalcoholic fatty liver disease (NAFLD) is a multifactorial disease caused by interactions between environmental and genetic factors. The SMXA-5 mouse is a high-fat diet-induced fatty liver model established from SM/J and A/J strains. We have previously identified Fl1sa, a quantitative trait locus (QTL) for fatty liver on chromosome 12 (centromere-53.06 Mb) of SMXA-5 mice. However, the chromosomal region containing Fl1sa was too broad. The aim of this study was to narrow the Fl1sa region by genetic dissection using novel congenic mice and to identify candidate genes within the narrowed Fl1sa region. RESULTS: We established two congenic strains, R2 and R3, from parental A/J-12SM and A/J strains. R2 and R3 strains have genomic intervals of centromere-29.20 Mb and 29.20-46.75 Mb of chromosome 12 derived from SM/J, respectively. Liver triglyceride content in R2 and R3 mice was significantly lower than that in A/J mice fed with a high-fat diet for 7 weeks. This result suggests that at least one of the genes responsible for fatty liver exists within the two chromosomal regions centromere-29.20 Mb (R2) and 29.20-46.75 Mb (R3). We found that liver triglyceride accumulation is inversely correlated with epididymal fat weight among the parental and congenic strains. Therefore, the ectopic fat accumulation in the liver may be due to organ-organ interactions between the liver and epididymal fat. To identify candidate genes in Fl1sa, we performed a DNA microarray analysis using the liver and epididymal fat in A/J and A/J-12SM mice fed with a high-fat diet for 7 weeks. In epididymal fat, mRNA levels of Zfp125 (in R2) and Nrcam (in R3) were significantly different in A/J-12SM mice from those in A/J mice. In the liver, mRNA levels of Iah1 (in R2) and Rrm2 (in R2) were significantly different in A/J-12SM mice from those in A/J mice. CONCLUSIONS: In this study, using congenic mice analysis, we narrowed the chromosomal region containing Fl1sa to two regions of mouse chromosome 12. We then identified 4 candidate genes in Fl1sa: Iah1 and Rrm2 from the liver and Zfp125 and Nrcam from epididymal fat.


Assuntos
Tecido Adiposo , Epididimo , Fígado Gorduroso/genética , Fígado , Locos de Características Quantitativas , Característica Quantitativa Herdável , Animais , Biomarcadores , Dieta Hiperlipídica , Fígado Gorduroso/sangue , Fígado Gorduroso/patologia , Expressão Gênica , Perfilação da Expressão Gênica , Estudos de Associação Genética , Masculino , Camundongos , Camundongos Congênicos , Fenótipo , RNA Mensageiro/genética , RNA Mensageiro/metabolismo
13.
Invest Ophthalmol Vis Sci ; 57(14): 6223-6233, 2016 11 01.
Artigo em Inglês | MEDLINE | ID: mdl-27842162

RESUMO

Purpose: To determine the preoperative characteristics and the changes in retinal blood flow following vitrectomy in eyes with a rhegmatogenous retinal detachment (RRD). Methods: Twenty-five-gauge vitrectomy without scleral bucking was performed on 31 eyes of 31 patients with macula-on RRD. The retinal blood flow on the optic nerve head (ONH) was assessed by laser speckle flowgraphy (LSFG), and the mean blur rate (MBR) and pulse waveform parameters before and at 10 days, 1, 2, 3, and 6 months after the surgery were examined. Eyes treated by scleral buckling, and eyes with an epiretinal membrane and cataract that underwent surgery were used as controls. Results: The mean preoperative MBR-vessel on the ONH was significantly lower in eyes with RRD than in the fellow unaffected eyes (P < 0.001), but it was not significantly different from the operated eye and the fellow eye in the control group. A significant increase in the mean MBR-vessel on the ONH was observed following vitrectomy in eyes affected by RRD (P < 0.001), whereas no significant difference was observed in the fellow eye, the scleral buckling-treated eyes, and the control eyes. Of the eight pulse waveform parameters, only the flow acceleration index was significantly lower in eyes with a RRD than in the fellow eyes preoperatively, but then it significantly increased with time following vitrectomy. The changes in the MBR-vessel were not correlated with that of other parameters (e.g., the ocular perfusion pressure). Conclusions: These results indicate that the retinal blood flow is reduced in eyes affected by RRD preoperatively, and can recover following successful RRD repair by vitrectomy.


Assuntos
Disco Óptico/irrigação sanguínea , Fluxo Sanguíneo Regional/fisiologia , Descolamento Retiniano/cirurgia , Vasos Retinianos/fisiopatologia , Acuidade Visual , Vitrectomia/métodos , Adulto , Idoso , Estudos Transversais , Feminino , Seguimentos , Humanos , Masculino , Microscopia Acústica , Pessoa de Meia-Idade , Período Pós-Operatório , Descolamento Retiniano/diagnóstico , Descolamento Retiniano/fisiopatologia , Vasos Retinianos/diagnóstico por imagem , Estudos Retrospectivos , Tomografia de Coerência Óptica
14.
Medicine (Baltimore) ; 95(43): e5102, 2016 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-27787364

RESUMO

The aim of the study was to investigate the relationship between choroidal blood flow and systemic and ocular variables in patients with healthy eyes.In this prospective cross-sectional study, we examined 241 eyes of 241 healthy Japanese subjects (92 males and 149 females; mean age, 37.8 ±â€Š17.0 years). The mean blur rate, a measure of the relative blood flow of the choroid, was determined using laser speckle flowgraphy. The total cross-sectional choroidal, luminal, and stromal areas of the choroid were determined by the binarization method. We investigated the correlation between choroidal MBR and systemic and ocular variables.Choroidal mean blur rate correlated with age (r = -0.385, P < 0.001) and choroidal thickness (r = 0.264, P < 0.001). The choroidal area correlated with choroidal mean blur rate (r = 0.374, P < 0.001), age (r = -0.184, P = 0.004), axial length (r = -0.251, P < 0.001), and choroidal thickness (r = 0.468, P < 0.001). The luminal area correlated with choroidal mean blur rate (r = 0.403, P < 0.001), age (r = -0.244, P < 0.001), axial length (r = -0.218, P = 0.001), and choroidal thickness (r = 0.435, P < 0.001). On multiple stepwise regression analyses, age (ß = -0.321, P < 0.001) and luminal area (ß = 0.320, P < 0.001), heart rate (ß = 0.136, P = 0.018), and mean ocular perfusion pressure (ß = 0.126, P = 0.045) were independent factors indicating the choroidal mean blur rate. Furthermore, axial length (ß = -0.352, P < 0.001), choroidal mean blur rate (ß = 0.273, P < 0.001), age (ß = -0.247, P < 0.001), gender (ß = -0.226, P < 0.001), and mean ocular perfusion pressure (ß = 0.193, P = 0.002) were independent factors indicating the luminal area.The choroidal blood flow positively correlated with the luminal area and negatively correlating with age. In addition, the luminal area was negative correlated with age. It is suggested that aging causes a reduction in choroidal blood flow and luminal area, and as a result of aging effect, decreased choroidal blood flow would correlate with decreased luminal area.


Assuntos
Envelhecimento/fisiologia , Pressão Sanguínea/fisiologia , Corioide/irrigação sanguínea , Pressão Intraocular/fisiologia , Fluxo Sanguíneo Regional/fisiologia , Adulto , Corioide/diagnóstico por imagem , Estudos Transversais , Feminino , Angiofluoresceinografia , Fundo de Olho , Voluntários Saudáveis , Humanos , Fluxometria por Laser-Doppler , Masculino , Estudos Prospectivos , Tomografia de Coerência Óptica
15.
Medicine (Baltimore) ; 95(32): e4317, 2016 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-27512843

RESUMO

The aim of this study was to compare changes in refraction following lens-sparing vitrectomy between patients with rhegmatogenous retinal detachment (RRD) and epiretinal membrane (ERM) and to investigate factors associated with the change in refraction.We reviewed medical records of 49 eyes of 49 patients with RRD (53.6 ±â€Š7.8 years, mean ±â€Šstandard deviation) and 24 eyes of 24 patients with ERM (50.9 ±â€Š15.7 years) who underwent lens-sparing vitrectomy. Spherical equivalent refractive power was evaluated before and up to 18 months after surgery. The relationship between the change in refraction and several parameters was evaluated.A significant progressive myopic shift in refractive power was observed after vitrectomy in operated RRD and ERM eyes (P < 0.001, P = 0.016, respectively), with no significant difference in fellow eyes. The refraction values observed at ≥3 and ≥12 months following vitrectomy were significantly different as compared with those observed at baseline in the RRD group (P < 0.001) and the ERM group (P < 0.05), respectively. The change in refraction between the RRD and ERM groups was significant (P = 0.030). The multiple linear regression analysis showed that only age was significantly correlated with the change in refraction in RRD (P = 0.018) and ERM (P < 0.001) groups. The change in refraction was significantly and positively correlated with age in RRD (r = -0.461, P = 0.001) and ERM (r = -0.687, P < 0.001) groups. Following lens-sparing vitrectomy, cataract surgery was performed on 30 eyes after 0.89 ±â€Š0.26 years in the RRD group and on 10 eyes after 1.11 ±â€Š0.14 years in the ERM group; there was a significant difference in time to cataract surgery between the groups (P = 0.007). Kaplan-Meier survival analysis demonstrated that there was a significant difference in the rate of cataract surgeries between the RRD and ERM groups (P = 0.022).Following lens-sparing vitrectomy for RRD and ERM, a progressive myopic shift in refraction owing to nuclear sclerosis was observed. Core vitrectomy itself would cause a myopic shift of refraction. The only risk factor associated with cataract progression following lens-sparing vitrectomy is age for both types of patients.


Assuntos
Catarata/patologia , Membrana Epirretiniana/cirurgia , Refração Ocular , Descolamento Retiniano/cirurgia , Vitrectomia/métodos , Fatores Etários , Estudos de Coortes , Membrana Epirretiniana/fisiopatologia , Feminino , Humanos , Estimativa de Kaplan-Meier , Modelos Lineares , Masculino , Pessoa de Meia-Idade , Análise Multivariada , Descolamento Retiniano/patologia , Estudos Retrospectivos , Medição de Risco , Resultado do Tratamento , Acuidade Visual
16.
Vet Immunol Immunopathol ; 175: 16-23, 2016 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-27269788

RESUMO

In avian species, maternal blood immunoglobulin Y (IgY) is transferred to the egg yolks of maturing oocytes, but the mechanism underlying this transfer is unknown. To gain insight into the mechanism of maternal IgY transfer in quail, we established an enzyme-linked immunosorbent assay (ELISA) for the quantitation of quail IgY. We characterized strain differences in blood and egg yolk IgY concentrations and exogenously injected IgY-Fc uptakes into egg yolks. A specific rabbit polyclonal antibody to quail IgY was raised for the ELISA. Blood and egg yolk IgY concentrations were determined in six quail strains (one inbred strain, L; four closed population strains, AWE, DB, PS, WE; one commercial strain, Commercial). The birds were also injected with digoxigenin-labeled quail IgY-Fc, and its uptakes into laid eggs were compared. The strain difference in blood and egg yolk IgY concentrations was at most 2.5-fold, between PS and AWE. The rank order of IgY concentrations was AWE, Commercial, DB, L≥WE≥PS. A significant positive correlation (|R|=0.786) between individual blood IgY and egg yolk IgY and the concentrated egg yolk IgY (1.5-2-fold) against blood IgY was observed. Interestingly, there was a significant inverse correlation (|R|=0.452) between injected IgY-Fc uptakes and the blood IgY concentration, implying competition of the injected IgY-Fc and blood IgY in the process of IgY uptake into egg yolks. In conclusion, we successfully determined blood and egg yolk IgY concentrations in various quail strains by a quail IgY-specific ELISA. The concentrated egg yolk IgY against the blood IgY and the inverse relationship of exogenous IgY-Fc uptake against the blood IgY supports the existence of a selective IgY transport mechanism in avian maturing oocytes.


Assuntos
Coturnix/imunologia , Gema de Ovo/imunologia , Ensaio de Imunoadsorção Enzimática/métodos , Imunoglobulinas/análise , Imunoglobulinas/metabolismo , Animais , Especificidade de Anticorpos , Feminino , Imunidade Materno-Adquirida , Fragmentos Fc das Imunoglobulinas/administração & dosagem , Fragmentos Fc das Imunoglobulinas/metabolismo , Imunoglobulinas/sangue , Coelhos , Proteínas Recombinantes/administração & dosagem , Proteínas Recombinantes/imunologia , Proteínas Recombinantes/farmacocinética , Especificidade da Espécie
17.
BMC Genet ; 17(1): 73, 2016 06 06.
Artigo em Inglês | MEDLINE | ID: mdl-27266874

RESUMO

BACKGROUND: The SMXA-5 mouse is an animal model of high-fat diet-induced fatty liver. The major QTL for fatty liver, Fl1sa on chromosome 12, was identified in a SM/J × SMXA-5 intercross. The SMXA-5 genome consists of the SM/J and A/J genomes, and the A/J allele of Fl1sa is a fatty liver-susceptibility allele. The existence of the responsible genes for fatty liver within Fl1sa was confirmed in A/J-12(SM) consomic mice. The aim of this study was to identify candidate genes for Fl1sa, and to investigate whether the identified genes affect the lipid metabolism. RESULTS: A/J-12(SM) mice showed a significantly lower liver triglyceride content compared to A/J mice when fed the high-fat diet for 7 weeks. We detected differences in the accumulation of liver lipids in response to the high-fat diet between A/J and A/J-12(SM) consomic mice. To identify candidate genes for Fl1sa, we performed DNA microarray analysis using the livers of A/J-12(SM) and A/J mice fed the high-fat diet. The mRNA levels of three genes (Iah1, Rrm2, Prkd1) in the chromosomal region of Fl1sa were significantly different between the strains. Iah1 mRNA levels in the liver, kidney, and lung were significantly higher in A/J-12(SM) mice than in A/J mice. The hepatic Iah1 mRNA level in A/J-12(SM) mice was 3.2-fold higher than that in A/J mice. To examine the effect of Iah1 on hepatic lipid metabolism, we constructed a stable cell line expressing the mouse Iah1 protein in mouse hepatoma Hepa1-6 cells. Overexpression of Iah1 in Hepa1-6 cells suppressed the mRNA levels of Cd36 and Dgat2, which play important roles in triglyceride synthesis and lipid metabolism. CONCLUSIONS: These results demonstrated that Fl1sa on the proximal region of chromosome 12 affected fatty liver in mice on a high-fat diet. Iah1 (isoamyl acetate-hydrolyzing esterase 1 homolog) was identified as one of the candidate genes for Fl1sa. This study revealed that the mouse Iah1 gene regulated the expression of genes related to lipid metabolism in the liver.


Assuntos
Cromossomos de Mamíferos/genética , Regulação da Expressão Gênica , Hepatopatia Gordurosa não Alcoólica/genética , Locos de Características Quantitativas/genética , Animais , Linhagem Celular , Dieta Hiperlipídica/efeitos adversos , Regulação da Expressão Gênica/efeitos dos fármacos , Metabolismo dos Lipídeos/efeitos dos fármacos , Metabolismo dos Lipídeos/genética , Fígado/efeitos dos fármacos , Fígado/metabolismo , Camundongos , Hepatopatia Gordurosa não Alcoólica/induzido quimicamente , Hepatopatia Gordurosa não Alcoólica/metabolismo , Fenótipo
18.
Invest Ophthalmol Vis Sci ; 57(3): 889-98, 2016 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-26943151

RESUMO

PURPOSE: The purpose of this study was to evaluate foveal regeneration and the association between retinal restoration and visual acuity following reattachment surgery for rhegmatogenous retinal detachment (RRD). METHODS: Twenty-nine eyes of 29 patients with successfully reattached macula-off RRD were retrospectively analyzed. We used spectral-domain optical coherence tomography to image macular regions and measure retinal thickness and Snellen chart visual acuity (VA) to evaluate best-corrected VA (BCVA) at 1, 2, 3, 6, 9, and 12 months after vitrectomy. Best-corrected visual acuity data were converted to the logarithm of the minimum angle of resolution scale. Opposite eyes were used as controls. RESULTS: The thicknesses of the external limiting membrane (ELM)-ellipsoid zone (EZ) and EZ-retinal pigment epithelium (RPE) were significantly thinner in involved eyes than in corresponding unaffected eyes at 1 month after surgery (P < 0.001 for both), and the thickness increased over time (P < 0.001 for both). Best-corrected visual acuity significantly improved over time (P < 0.001), and the improvement correlated with EZ-RPE thickness (r = -0.45, P = 0.021). Multiple regression analysis demonstrated the presence of a foveal bulge as the independent predictor of final BCVA (P < 0.001). Eyes with a foveal bulge had significantly better BCVA and greater EZ-RPE thickness than those without throughout the follow-up period. Significant restoration of the integrity of EZ and cone interdigitation zone (CIZ) was observed over time (P < 0.001 for both) in eyes with a foveal bulge. CONCLUSIONS: The thickness of EZ-RPE and cone density increased during foveal regeneration, as demonstrated by the continuous improvements in CIZ integrity over time, leading to the formation of foveal bulge and good vision following successful reattachment of macula-off RRD.


Assuntos
Células Fotorreceptoras de Vertebrados/fisiologia , Regeneração , Descolamento Retiniano/patologia , Acuidade Visual/fisiologia , Vitrectomia , Feminino , Seguimentos , Fóvea Central/patologia , Humanos , Masculino , Pessoa de Meia-Idade , Período Pós-Operatório , Descolamento Retiniano/fisiopatologia , Descolamento Retiniano/cirurgia , Estudos Retrospectivos , Tomografia de Coerência Óptica
19.
Vascular ; 24(5): 523-30, 2016 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-26767606

RESUMO

AIM: To describe the long-term experience of a simplified frozen elephant trunk technique (sFETT) used in complicated acute type A aortic dissection (AAAD) treatment. METHODS AND RESULTS: Between January 2001 and December 2012, 34 patients (mean age 59.9 ± 11.0 years) with complicated AAAD (DeBakey I) underwent an emergency surgery including sFETT. sFETT consisted in gluing the dissected aortic arch wall layers with gelatine-resorcinol adhesive and video-assisted antegrade open arch aortic stent-graft deployment in the arch or proximal descending aorta. In addition to sFETT, the aortic root was addressed with standard techniques. A 30-day mortality was 14.7% (five patients) due to bleeding (1), multiple organ failure (2), and colon ischemia (2). Postoperative morbidity included neurological (2), renal (1) and cardio-pulmonary complications (4), as well as wound infection (1). Mean follow-up was 74.4 ± 45.0 months. Actual survival rates were 73.5% at 1 year, 70.2% at 5 years, and 58.5% at 13 years of follow-up. Six patients died during long-term follow-up from heart failure (1) and unknown reasons (5). Five patients required reoperation for aortic arch (3) or aorto-iliac (2) progression of aneurysm during the mid- and long-term follow-up. The remaining patients showed favorable evolution of the dissected aorta with false lumen occlusion in most cases and stable aortic diameters. CONCLUSIONS: In AAAD patients, sFETT as used in our series is an easy and safe technique to repair the aortic arch. Long-term results after sFETT showed false lumen occlusion and stable aortic diameter in up to 13 years of follow-up.


Assuntos
Aneurisma Dissecante/cirurgia , Aneurisma Aórtico/cirurgia , Implante de Prótese Vascular/métodos , Procedimentos Endovasculares/métodos , Doença Aguda , Adulto , Idoso , Idoso de 80 Anos ou mais , Aneurisma Dissecante/diagnóstico por imagem , Aneurisma Dissecante/mortalidade , Aneurisma Aórtico/diagnóstico por imagem , Aneurisma Aórtico/mortalidade , Aortografia/métodos , Prótese Vascular , Implante de Prótese Vascular/efeitos adversos , Implante de Prótese Vascular/instrumentação , Implante de Prótese Vascular/mortalidade , Angiografia por Tomografia Computadorizada , Emergências , Procedimentos Endovasculares/efeitos adversos , Procedimentos Endovasculares/instrumentação , Procedimentos Endovasculares/mortalidade , Feminino , Humanos , Estimativa de Kaplan-Meier , Masculino , Pessoa de Meia-Idade , Complicações Pós-Operatórias/mortalidade , Complicações Pós-Operatórias/cirurgia , Desenho de Prótese , Reoperação , Estudos Retrospectivos , Fatores de Risco , Stents , Fatores de Tempo , Adesivos Teciduais/uso terapêutico , Resultado do Tratamento , Cirurgia Vídeoassistida
20.
Anim Sci J ; 87(2): 257-65, 2016 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-26304689

RESUMO

Recent studies have emphasized the crucial role of gut microbiota in triggering and modulating immune response. We aimed to determine whether the modification of gut microbiota by oral co-administration of two antibiotics, ampicillin and neomycin, would lead to changes in the antibody response to antigens in chickens. Neonatal chickens were given or not given ampicillin and neomycin (0.25 and 0.5 g/L, respectively) in drinking water. At 2 weeks of age, the chicks were muscularly or orally immunized with antigenic keyhole limpet hemocyanin (KLH), and then serum anti-KLH antibody levels were examined by ELISA. In orally immunized chicks, oral antibiotics treatment enhanced antibody responses (IgM, IgA, IgY) by 2-3-fold compared with the antibiotics-free control, while the antibiotics did not enhance antibody responses in the muscularly immunized chicks. Concomitant with their enhancement of antibody responses, the oral antibiotics also lowered the Lactobacillus species in feces. Low doses of antibiotics (10-fold and 100-fold lower than the initial trial), which failed to change the fecal Lactobacillus population, did not modify any antibody responses when chicks were orally immunized with KLH. In conclusion, oral antibiotics treatment enhanced the antibody response to orally exposed antigens in chickens. This enhancement of antibody response was associated with a modification of the fecal Lactobacillus content, suggesting a possible link between gut microbiota and antibody response in chickens.


Assuntos
Ampicilina/administração & dosagem , Ampicilina/farmacologia , Antibacterianos/administração & dosagem , Antibacterianos/farmacologia , Anticorpos/sangue , Formação de Anticorpos/efeitos dos fármacos , Galinhas/imunologia , Hemocianinas/imunologia , Neomicina/administração & dosagem , Neomicina/farmacologia , Animais , Carga Bacteriana/efeitos dos fármacos , Ensaio de Imunoadsorção Enzimática , Fezes/microbiologia , Trato Gastrointestinal/imunologia , Trato Gastrointestinal/microbiologia , Injeções Intramusculares , Lactobacillus/efeitos dos fármacos , Estimulação Química
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