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1.
Bull Exp Biol Med ; 152(4): 528-34, 2012 Feb.
Artigo em Inglês, Russo | MEDLINE | ID: mdl-22803127

RESUMO

Regeneration processes in rat mandibular bone after transplantation of a suspension of autologous BM MSC in culture medium were studied by methods of light microscopy and X-ray densitometry. It was found that the structures of red BM in the callus after transplantation of autologous BM MSC formed earlier than in natural reparation. The formation of cavities containing BM determines lower tissue density at the site of injury after transplantation of autologous BM MSC on weeks 4 and 5 of observation than during spontaneous healing. These changes progressed throughout the observation period and attested to accelerated bone tissue reparation.


Assuntos
Regeneração Óssea , Mandíbula/patologia , Traumatismos Mandibulares/terapia , Transplante de Células-Tronco Mesenquimais , Absorciometria de Fóton , Animais , Medula Óssea/fisiologia , Masculino , Traumatismos Mandibulares/patologia , Células-Tronco Mesenquimais/citologia , Células-Tronco Mesenquimais/fisiologia , Microscopia , Ratos , Transplante Autólogo , Cicatrização
2.
Stomatologiia (Mosk) ; 91(1): 5-8, 2012.
Artigo em Russo | MEDLINE | ID: mdl-22678598

RESUMO

The regeneration processes after introduction in site of damaged bone of rat bottom jaw of autologous mesenchymal stem cells of bone marrow origin (AMSCBMO) in suspension were studied by methods of light microscopy and x-ray densitometry. Morphological methods showed that formation of red bone marrow in bone callosity occurs much earlier after use AMSCBMO than in the time of natural course of reparation. Formation of cavities with a marrow decreases tissue density after application AMSCBMO in a site of damage in 4 and 5 weeks of supervision. The specified changes progress during all time of observation and are the certificate of accelerated development of regenerative processes in bone tissue.


Assuntos
Regeneração Óssea , Mandíbula/fisiologia , Transplante de Células-Tronco Mesenquimais , Células-Tronco Mesenquimais/fisiologia , Animais , Medula Óssea/fisiologia , Masculino , Mandíbula/citologia , Mandíbula/diagnóstico por imagem , Mandíbula/cirurgia , Traumatismos Mandibulares/cirurgia , Células-Tronco Mesenquimais/citologia , Osteogênese , Radiografia , Ratos , Ratos Endogâmicos , Transplante Autólogo
3.
Stomatologiia (Mosk) ; 90(4): 9-12, 2011.
Artigo em Russo | MEDLINE | ID: mdl-21983607

RESUMO

The processes of regeneration of the damaged rat bottom jaw bone after application of enriched thrombocytes a fibrin clot were studied by morphological and radiovisiographic methods. At a natural course of regeneration the artificial aperture of bone was filled with blood and there the blood clot was formed. After 1 week the separate bone islets of a young tissue occurred in bone defect. In 2-3 weeks the aperture in a bottom jaw bone was completely closed by a young bone tissue. After operation with filling of bone bottom jaw defect by fibrin clot there was no formation of a blood clot. Already after 1 week the bone tissue defect was filled by the merged islets of again generated bone. By second week after fibrin use the further formation of bone tissue in defect and formation of a bone callosity was noted.


Assuntos
Regeneração Óssea/efeitos dos fármacos , Produtos de Degradação da Fibrina e do Fibrinogênio/administração & dosagem , Mandíbula/fisiologia , Mandíbula/cirurgia , Animais , Plaquetas/fisiologia , Masculino , Mandíbula/citologia , Ratos , Ratos Endogâmicos
4.
Stomatologiia (Mosk) ; 90(5): 60-3, 2011.
Artigo em Russo | MEDLINE | ID: mdl-22332384

RESUMO

Recent clinical and experimental researches on application of mesenchymal stem cells (MSC) in dentistry and maxillofacial surgery showed the efficiency of cellular technologies for acceleration of tissue regeneration and implants integration. However use of MSC solely and in combination with other substances has its advantages and disadvantages. There is lack of scientific data on contraindications and complications of cellular therapy whereas the analysis of failures of MSC application will allow accurately define indications and raise the efficiency of cellular technologies. There is a strong need for further clinical and experimental studies on MSC application for bone tissue regeneration.


Assuntos
Regeneração Óssea , Transplante de Células-Tronco Mesenquimais , Células-Tronco Mesenquimais/fisiologia , Procedimentos Cirúrgicos Bucais , Humanos , Células-Tronco Mesenquimais/citologia , Células-Tronco Mesenquimais/metabolismo
5.
Morfologiia ; 140(6): 79-85, 2011.
Artigo em Russo | MEDLINE | ID: mdl-22506358

RESUMO

The processes of the repair of the damaged mandibular bone in rats were studied using light microscopy and x-ray densitometry at various time intervals after the local injection of the platelet-rich fibrin clot (PRFC), autologous mesenchymal (stromal) stem cells of bone marrow origin (AMSCBMO) or AMSCBMO, adsorbed on PRFC, into the damaged site. The best results were obtained after the application of PRFC with AMSCBMO. One week after the operation, the mandibular bone defect was largely filled with the newly formed bone tissue. It seems most probable that in this case the effects of fibrin and stem cells on the damaged bone were summarized or even amplified. Bone formation in these cases appeared to begin in the center, but not at the edges, of the defect. AMSCBMO were distributed over the whole volume of PRFC, filling all the defect more or less uniformly. As a result, maximally fast and successful restoration of bone tissue was reached in the area of the defect.


Assuntos
Células da Medula Óssea/citologia , Regeneração Óssea , Traumatismos Mandibulares/terapia , Transplante de Células-Tronco Mesenquimais , Animais , Fibrina/metabolismo , Células-Tronco Mesenquimais/citologia , Plasma Rico em Plaquetas/citologia , Ratos , Transplante Autólogo
6.
Bull Exp Biol Med ; 152(1): 112-9, 2011 Nov.
Artigo em Inglês, Russo | MEDLINE | ID: mdl-22803055

RESUMO

Scientific literature about the use of MSC contains clinical and experimental data on the efficiency of cell technologies for restoration of the osteoarticular apparatus. The use of MSC immobilized in the appropriate carriers and differentiation of these cells towards the bone cells and chondrocytes are of crucial importance. However, the use of MSC, both individual and in combination with other preparations and substances has a number of drawbacks and advantages. The absence of published reports on contraindications and complications of cell therapy is worthy of note, because the analysis of unsuccessful application of MSC will help to determine the indication for this treatment, and hence, to improve the efficiency of cell technologies in the future. Wider use of MSC in clinical practice and experimental studies for acceleration of reparative processes in the bone and cartilage tissue seems to be promising.


Assuntos
Regeneração Óssea , Cartilagem/fisiologia , Transplante de Células-Tronco Mesenquimais , Animais , Diferenciação Celular , Humanos , Imunomodulação , Artropatias/terapia , Células-Tronco Mesenquimais/imunologia , Células-Tronco Mesenquimais/fisiologia , Doenças da Coluna Vertebral/terapia
7.
Stomatologiia (Mosk) ; 89(5): 11-4, 2010.
Artigo em Russo | MEDLINE | ID: mdl-21191330

RESUMO

The structure of submandibular lymph nodes (LN) from rats with arterial hypertension (AH) and normal pressure after mandible damage was investigated by methods of light microscopy. It was revealed that in submandibular LN of both compared groups of animals after induction of inflammatory reaction cortical field was extended, bulk density of lymphoid nodules without reproductive centres was increased, in nodules with centres mantle was extended and germinative centres were decreased. In all structures of LN the numbers of mitosis, imrnuno - and plasmoblasts were increased, the contents of reticular cells and macrophages were increased only to 4-5 week. In structure of LN from rats with normal arterial pressure and with AH the volume of sinus system statistically significantly differed: animals with AH had more volume of sinuses on all points of observation. Inflammation in the conditions of AH the distinction in cellular content of various zones of LN were connected with occurrence or higher number of erythrocytes.


Assuntos
Hipertensão/complicações , Inflamação/patologia , Linfonodos/patologia , Doenças Mandibulares/patologia , Animais , Inflamação/complicações , Macrófagos/patologia , Masculino , Doenças Mandibulares/complicações , Plasmócitos/patologia , Ratos , Ratos Wistar
8.
Morfologiia ; 135(2): 43-7, 2009.
Artigo em Russo | MEDLINE | ID: mdl-19563173

RESUMO

The structure of leukocyte infiltrates in gingiva of 80 patients of various ages and gender with chronic apical periodontitis was studied using light microscopy with the application of monoclonal antibodies detecting CD38 antigen. Gingival tissues of practically of all the patients with periodontitis contained 2 types of leukocyte infiltrates: infiltrates with the low plasma cell content and high numbers of neutrophils and structures with high number of plasmocytes and low concentration of neutrophilic granulocytes. Within the gingival epithelium in patients with chronic apical periodontitis CD38 + cells were absent. The histogenesis of gingival leukocyte infiltrates in chronic apical periodontitis is discussed with the special emphasis on the role of plasma cells in the development of the pathological process.


Assuntos
Periodontite Crônica/patologia , Gengiva/patologia , Tecido Periapical/patologia , Plasmócitos/patologia , ADP-Ribosil Ciclase 1/imunologia , Adolescente , Adulto , Periodontite Crônica/imunologia , Feminino , Gengiva/imunologia , Humanos , Imuno-Histoquímica , Contagem de Linfócitos , Masculino , Pessoa de Meia-Idade , Infiltração de Neutrófilos/imunologia , Tecido Periapical/imunologia , Plasmócitos/citologia , Plasmócitos/imunologia , Adulto Jovem
9.
Stomatologiia (Mosk) ; 88(2): 29-33, 2009.
Artigo em Russo | MEDLINE | ID: mdl-19491780

RESUMO

By light microscopy there was studied gingival tissues structure depending upon age and gender in 96 relatively healthy persons and 80 patients with chronic apical periodontitis. It was disclosed that in gingival mucosa of patients with chronic apical periodontitis the interstitial space volumetric density and numerical density of tissue leucocytes increased in the first place due to neutrophils. Between the healthy men and women there was no significant difference in structural organization of the gum as well as between the patients of different gender. The only sign was that in healthy men and women with age progressing only lymphatic vessels area decreased on gingival mucosa microscopic section. In patients with chronic apical periodontitis both blood and lymphatic vessels were involved through decrease of relative capillary density.


Assuntos
Gengiva/patologia , Periodontite/patologia , Adolescente , Adulto , Fatores Etários , Feminino , Gengiva/irrigação sanguínea , Humanos , Leucócitos/patologia , Vasos Linfáticos/patologia , Masculino , Microcirculação , Pessoa de Meia-Idade , Fatores Sexuais , Adulto Jovem
10.
Stomatologiia (Mosk) ; 88(1): 9-13, 2009.
Artigo em Russo | MEDLINE | ID: mdl-19365340

RESUMO

In gingival tissues of 62 patients after dental implantation of traditional type and with the use of thrombocyte rich fibrin preparation (TRFP) microcirculation and leucocytal infiltration were studied. It was disclosed that in all terms after titanium screw dental implant setting lymphostasis and leucocytal infiltration were seen as signs of active inflammatory process in gingival tissues. 3 months later after implants setting with the use of TRFP the lymphatic vessels status was normalized, the degree of leucocytal infiltration was reduced; when implantation was made without use of fibrin technologies in gingival tissues more leucocytes were found and lymphatic bed components were remained dilated. Besides fibrin preparation use in the process of dental implantation promoted quicker and stronger implant fixation, but granulomatous inflammatory process could develop and amount of eosinophils in gingival tissues could also increased.


Assuntos
Implantação Dentária/métodos , Fibrina/administração & dosagem , Gengiva/patologia , Gengiva/cirurgia , Leucócitos/patologia , Adulto , Feminino , Gengiva/irrigação sanguínea , Humanos , Linfa , Masculino , Microcirculação , Pessoa de Meia-Idade , Granuloma Periapical/patologia
11.
Stomatologiia (Mosk) ; 87(1): 31-8, 2008.
Artigo em Russo | MEDLINE | ID: mdl-18427478

RESUMO

Radiovisiographic study of periodontal tissues changes in different periods after the therapy of destructive chronic periodontitis have disclosed that jaw alveolar process bone density and the size of the destruction focus after 6 months, 1 and 2 years after the treatment were not significantly changed (134 patients sample of different gender and age). When this sample was subdivided into groups depending upon gender and age some significant changes of some parameters were found. The most expressed were the changes of the size of the periodontal destruction focus in the patients of 40-49 years of age, less pronounced they were in patients of 18-29 age group; in the age group 30-39 years changes were not found, In men of 18-29 age group regenerative processes in periodontal tissues were more expressed than in women. In patients of female sex of the age group 30-39 years were found more expressed changes. In older age group in patients of both genders the changes were approximately equal. The bone density around periodontium after treatment in men of the age group 18-29 years was restored more intensive than in 40-49-year-old patients. In females age dependent changes in the studied tissues status were not found.


Assuntos
Periodontite/patologia , Periodonto/fisiologia , Regeneração/fisiologia , Adolescente , Adulto , Fatores Etários , Doença Crônica , Feminino , Seguimentos , Humanos , Masculino , Pessoa de Meia-Idade , Periodontite/diagnóstico por imagem , Periodontite/terapia , Prognóstico , Radiografia , Índice de Gravidade de Doença , Fatores de Tempo
15.
Vopr Onkol ; 30(9): 43-6, 1984.
Artigo em Russo | MEDLINE | ID: mdl-6495692

RESUMO

Due to the difficulties involved in establishing an early diagnosis, advanced cases make up about 70% of lung cancer patients admitted to specialized surgical wards. According to more than 30 year-old experience gained at the Academy's Hospital Surgical Clinic and evaluation of the end results of extensive and combined resection of the lung for the treatment of cancer, over 26% of cases operated on at the advanced stages of the disease survive for more than 5 years. Special surgical procedures should be carried out in such cases.


Assuntos
Neoplasias Pulmonares/cirurgia , Pneumonectomia/métodos , Brônquios/cirurgia , Humanos , Neoplasias Pulmonares/mortalidade , Neoplasias Pulmonares/patologia , Artéria Pulmonar/cirurgia , Veias Pulmonares/cirurgia
19.
Vopr Onkol ; 28(8): 20-5, 1982.
Artigo em Russo | MEDLINE | ID: mdl-7113065

RESUMO

The end results of surgical and combination therapy of lung cancer are compared on the basis of rates of survival for 5 years and longer. Extended resection of lung including the removal of lymph nodes and mediastinal fat tissue was performed in all cases. Such megavolt radiation sources as Lootch, Rokus, Lu-4, 3 Mev were used in postoperative radiation treatment. No significant differences in the end results of the two schemes of treatment were found in cases of stage I-II tumors. Survival for 5 years and longer were registered in 30.2% of patients with stage III tumor after combination therapy and 25.9% of patients treated by surgery alone. Surgical treatment of extensive stage III tumor should be followed by radiation.


Assuntos
Adenocarcinoma/terapia , Carcinoma de Células Escamosas/terapia , Neoplasias Pulmonares/terapia , Complicações Pós-Operatórias , Adenocarcinoma/mortalidade , Adenocarcinoma/patologia , Carcinoma de Células Escamosas/mortalidade , Carcinoma de Células Escamosas/patologia , Humanos , Neoplasias Pulmonares/mortalidade , Neoplasias Pulmonares/patologia , Excisão de Linfonodo , Metástase Linfática , Estadiamento de Neoplasias , Pneumonectomia , Dosagem Radioterapêutica
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