Your browser doesn't support javascript.
Mostrar: 20 | 50 | 100
Resultados 1 - 9 de 9
Filtrar
Mais filtros










Base de dados
Intervalo de ano de publicação
1.
Biomed Pharmacother ; 117: 109204, 2019 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-31387177

RESUMO

We wished to investigate the role of a tilapia skin collagen polypeptide (TSCP; molecular weight <3 kDa) in alleviating liver and kidney injuries in aging mice induced by d-galactose (d-gal) and its underlying mechanism of action. First, we characterized TSCP. TSCP was passed through a 3-kDa ultrafiltration membrane, desalted in water by a solid-phase extraction column, purified further by reverse phase-high performance liquid chromatography, and analyzed by electrospray ionization mass spectrometry and tandem mass spectrometry. TSCP contained 17 types of amino acids (AAs) and 41 peptide chains of length 7 AAs to 22 AAs. The content of free AAs and total AAs of TSCP was 13.5% and 93.79%, respectively. Next, we undertook animal experiments. Mice were injected once-daily with D-gal (300 mg/kg body weight, s.c.) for 8 weeks, and TSCP was administered simultaneously once-daily by intragastric gavage. TSCP could visibly improve the decreased body weight, depressed appetite, and mental deterioration of mice triggered by d-gal. TSCP could also alleviate d-gal-induced damage to the liver and kidneys according to histopathology (especially high-dose TSCP). Consistent with these macroscopic and pathologic changes, TSCP could also prevent d-gal-induced increases in serum levels of alanine aminotransferase, aspartate transaminase, alkaline phosphatase, lipid peroxidation, creatinine and uric acid, as well as decreases in serum levels of immunoglobulin (Ig)G and IgM. Moreover, TSCP improved the activities of superoxide dismutase, catalase, and glutathione peroxidase, but also inhibited the increases in the levels of malondialdehyde and inducible nitric oxide synthase expression in the liver and kidneys of d-gal-treated mice. These results suggest that TSCP can alleviate the injuries to the liver and kidneys in aging mice induced by d-gal, and that its mechanism of action might be, at least partially, associated with attenuation of oxidative stress and enhancement of immune function.

2.
Mar Drugs ; 16(6)2018 May 24.
Artigo em Inglês | MEDLINE | ID: mdl-29794973

RESUMO

Chitosan oligosaccharide (COS), a natural polysaccharide with good antioxidant and anti-inflammatory properties, is the depolymerized product of chitosan possessing various biological activities. The present study was designed to investigate the possible anti-aging effect of COS on the aging model mouse induced by d-galactose (d-gal) and explore the underlying mechanism. In the experiment, 48 male Kunming mice (KM mice) were randomly divided into the normal group, model group, positive group, and low-medium-high dose polysaccharide groups (300, 600, 1200 mg/kg/day). The results showed that COS, by intragastric gavage after subcutaneous injection of d-gal (250 mg/kg/day) into the neck of mice consecutively for eight weeks, gradually recovered the body weight, the activity of daily living, and organ indices of mice, as well as effectively ameliorated the histological deterioration of the liver and kidney in mice triggered by d-gal. To be specific, COS obviously improved the activities of antioxidant enzymes in liver and kidney of KM mice, including catalase (CAT), glutathione peroxidase (GSH-Px), and superoxide dismutase (SOD), as well as decreased malondialdehyde (MDA) levels when compared with those in model group mice. Furthermore, COS not only elevated the diminished levels of serum immunoglobulin G (IgG) and IgM induced by d-gal, but also significantly inhibited the d-gal-caused upregulation of serum alanine aminotransferase (ALT), aspartate transaminase (AST), alkaline phosphatase (ALP), uric acid (UA) and creatinine (CREA) levels as compared with those of mice in the model group. These results demonstrate that COS has an obvious anti-aging activity in d-gal-induced subacute aging mice, the mechanism of which, to some extent, is associated with enhancing the antioxidant defenses, reducing oxidative stress, and improving the immune function of aging model mice.


Assuntos
Envelhecimento/efeitos dos fármacos , Antioxidantes/farmacologia , Quitosana/farmacologia , Oligossacarídeos/farmacologia , Estresse Oxidativo/efeitos dos fármacos , Envelhecimento/imunologia , Alanina Transaminase/sangue , Animais , Aspartato Aminotransferases/sangue , Peso Corporal/efeitos dos fármacos , Catalase/metabolismo , Galactose/imunologia , Glutationa Peroxidase/metabolismo , Sistema Imunitário/efeitos dos fármacos , Imunoglobulina G/sangue , Imunoglobulina M/sangue , Rim/efeitos dos fármacos , Rim/patologia , Fígado/efeitos dos fármacos , Fígado/enzimologia , Fígado/patologia , Masculino , Malondialdeído/sangue , Camundongos , Modelos Animais , Superóxido Dismutase/metabolismo
3.
Exp Gerontol ; 103: 27-34, 2018 03.
Artigo em Inglês | MEDLINE | ID: mdl-29275159

RESUMO

Skin photoaging (SP) is a premature skin-aging damage after repeated exposure to ultraviolet (UV) radiation, mainly characterized by oxidative stress and inflammatory disequilibrium, which makes skin show the typical symptoms of photoaging such as coarse wrinkling, dryness, irregular pigmentation and laxity. Chitosan oligosaccharide (COS), a natural polysaccharide with good humectant property, is the depolymerized product of chitosan with various biological activities, among which the antioxidant and anti-inflammatory effects have been frequently reported in recent years. However, no existing invivo study indicates whether COS has direct protective effect on UV-induced SP. In the current research, we investigated the potential preventive effect of COS against UV-caused damage in hairless mouse dorsal skin. The data showed that COS, by topical application after each UV-radiation for 10weeks, effectively inhibited the undesirable changes on the skin induced by UV. To be specific, COS obviously alleviated the macroscopic and histopathological damages of mice skin, via mitigating the disrupted collagenous fibers, as well as improving the relative content of type I collagen and the amount of total collagen. Furthermore, COS effectively inhibited the levels of pro-inflammatory cytokines such as TNF-α, IL-1ß and IL-6, and markedly improved the activities of antioxidant enzymes (SOD, GSH-Px, CAT), as well as the content of skin hydroxyproline and moisture. These findings demonstrated that this natural polysaccharide attenuated UV-induced SP, at least in part, by virtue of favorable regulation of antioxidant and anti-inflammatory status, which presumably worked in concert to maintain the morphology and level of dermal collagen.

4.
Exp Gerontol ; 61: 147-55, 2015 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-25498537

RESUMO

It has been confirmed that repeated exposure of skin to ultraviolet (UV) radiation results in cutaneous oxidative stress and inflammation, which act in concert to cause premature skin aging, well known as photoaging. 18ß-Glycyrrhetinic acid (GA), widely used to treat various tissue inflammations, is the main active component of licorice root, and has also been shown to possess favorable anti-oxidative property and modulating immunity function. In the present study, we investigated the potential protective effect of GA on UV-induced skin photoaging in a mouse model. During the experimental period of ten consecutive weeks, the dorsal depilated skin of mice was treated with topical GA for 2 hours prior to UV irradiation. The results showed that GA pretreatment significantly alleviated the macroscopic and histopathological damages in mice skin caused by UV. Meanwhile, the data also indicated that GA markedly up-regulated the activities of the antioxidant enzymes (SOD, GSH-Px), and increased the content of skin collagen, while obviously decreased malonaldehyde level and inhibited high expressions of matrix metalloproteinase-1 (MMP-1) and -3 (MMP-3), as well as down-regulated the expression of inflammatory cytokines such as IL-6, TNF-α and IL-10. Taken together, these findings amply demonstrate that GA observably attenuates UV-induced skin photoaging mainly by virtue of its antioxidative and anti-inflammatory properties, as well as regulating the abnormal expression of MMP-1 and MMP-3.


Assuntos
Ácido Glicirretínico/análogos & derivados , Protetores contra Radiação/farmacologia , Envelhecimento da Pele/efeitos dos fármacos , Animais , Elasticidade , Feminino , Glutationa Peroxidase/metabolismo , Ácido Glicirretínico/farmacologia , Malondialdeído/análise , Metaloproteinase 3 da Matriz/metabolismo , Camundongos , Pele/metabolismo , Pele/patologia , Pele/efeitos da radiação , Superóxido Dismutase/metabolismo , Raios Ultravioleta
5.
Eur J Pharm Sci ; 63: 113-23, 2014 Oct 15.
Artigo em Inglês | MEDLINE | ID: mdl-25033712

RESUMO

Ultraviolet (UV) irradiation, known to generate reactive oxygen species (ROS) excessively and elicit inflammatory response, is a potent inducer for skin photoaging. Overproduction of ROS in conjunction with the resulting inflammation stimulate the over-expression of matrix metalloproteinases (MMPs), which in turn causes degradation of extracellular matrix, leading finally to coarse wrinkling, dryness, and laxity of the skin. In this study, patchouli alcohol (PA, C15H26O), an active chemical ingredient reputed for free radical scavenging and anti-inflammatory properties, was investigated for its anti-photoaging action using a mouse model whose dorsal skin was depilated. The dorsal skin areas of six-week-old mice were smeared with PA solution or vehicle, followed by UV irradiation for nine consecutive weeks. Protective effects of PA were evaluated macroscopically and histologically, as well as by assaying the antioxidant enzymes (SOD, GSH-Px) activities, the contents of inflammatory factors (IL-10, IL-6, TNF-α), and the levels of MMP-1 and MMP-3. Our findings amply demonstrated that PA significantly accelerated the recovery of the UV-induced skin lesions, evidently through anti-oxidant and anti-inflammatory action, as well as down-regulation of the MMP-1 and MMP-3 expression.


Assuntos
Sesquiterpenos/administração & dosagem , Sesquiterpenos/farmacologia , Envelhecimento da Pele/efeitos dos fármacos , Envelhecimento da Pele/efeitos da radiação , Raios Ultravioleta , Administração Tópica , Animais , Feminino , Camundongos , Camundongos Endogâmicos , Testes Cutâneos
6.
Pest Manag Sci ; 70(3): 510-6, 2014 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-23966130

RESUMO

BACKGROUND: Essential oil of Pogostemon cablin (Blanco) Benth. has been reported to exhibit strong insecticidal activities, but few studies have focused on the insecticidal activity of its main individual constituent, pogostone (PO). The goal of this research was to investigate the insecticidal activity of PO against two harmful noctuid insects, Spodoptera litura (Fabricius) and Spodoptera exigua (Hübner). RESULTS: In a no-choice assay, PO exhibited strong antifeedant activity against S. litura and S. exigua. PO showed pronounced larvicidal activities, including oral toxicity (LC50 986.88 mg L(-1) and 545.61 mg L(-1) respectively) and contact toxicity (LC50 1041.42 mg L(-1) and 519.48 mg L(-1) respectively) against these two noctuid insects. Additionally, PO treatment significantly increased the larval and pupal developmental period. Furthermore, PO showed moderate ovicidal activities and influenced the emergence and deformity of the moth. However, PO failed to exert a potent effect on adult development. These tested parameters proved to be dose dependent for both insect species. CONCLUSION: PO possesses strong insecticidal activities, especially antifeedant, larvicidal, growth inhibitory and pupicidal activities, against S. litura and S. exigua. PO may partly account for the insecticidal activity of patchouli oil and may be a promising candidate for the control of agricultural insects.


Assuntos
Inseticidas/farmacologia , Lamiaceae/química , Óleos Voláteis/farmacologia , Extratos Vegetais/farmacologia , Spodoptera/efeitos dos fármacos , Animais , Comportamento Alimentar/efeitos dos fármacos , Feminino , Larva/efeitos dos fármacos , Larva/crescimento & desenvolvimento , Larva/fisiologia , Masculino , Pupa/efeitos dos fármacos , Pupa/crescimento & desenvolvimento , Pupa/fisiologia , Spodoptera/crescimento & desenvolvimento , Spodoptera/fisiologia
7.
Rejuvenation Res ; 16(5): 404-13, 2013 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-23822553

RESUMO

Chronic exposure to ultraviolet (UV) irradiation is believed to be the major cause of skin damage that results in premature aging of the skin, so called photoaging, characterized by increases in skin thickness, formation of wrinkles, and loss of skin elasticity. UV induces damage to skin mainly by oxidative stress and collagen degradation. In this study, we examined the photo-protective effect of hydroxysafflor yellow A (HSYA), a major active chemical component isolated from Carthamus tinctorius L., by topical application on the skin of mice. Exposure of the dorsal depilated skin of mice to UV radiation four times a week for 10 weeks induced epidermal hyperplasia, elastin accumulation, collagen degradation, etc. HSYA at the doses of 50, 100, and 200 µg/mouse was topically applied immediately following each UV exposure. The effects of HSYA were evaluated by a series of tests, including macroscopic and histopathological evaluation of skin, pinch test, and redox homeostasis of skin homogenates. Results showed that the UV-induced skin damage was significantly improved after HSYA treatment, especially at doses of 100 and 200 µg/mouse. This protective effect is possibly related to the anti-oxidative property of HSYA and mediated by promoting endogenous collagen synthesis. This is the first study providing preclinical evidence for the protective effect of HSYA against photoaging.


Assuntos
Chalcona/análogos & derivados , Quinonas/farmacologia , Envelhecimento da Pele/efeitos dos fármacos , Envelhecimento da Pele/efeitos da radiação , Pele/patologia , Raios Ultravioleta , Animais , Antioxidantes/metabolismo , Proliferação de Células/efeitos dos fármacos , Chalcona/química , Chalcona/isolamento & purificação , Chalcona/farmacologia , Colágeno/metabolismo , Epiderme/efeitos dos fármacos , Epiderme/patologia , Epiderme/efeitos da radiação , Feminino , Malondialdeído/metabolismo , Camundongos , Quinonas/química , Quinonas/isolamento & purificação , Pele/efeitos dos fármacos , Pele/efeitos da radiação , Testes Cutâneos , Coloração e Rotulagem
8.
Neurochem Res ; 38(5): 951-60, 2013 May.
Artigo em Inglês | MEDLINE | ID: mdl-23420419

RESUMO

The accumulation of extracellular amyloid-ß peptide (Aß) has been considered as one of the important causes of Alzheimer's disease (AD), the most prevalent form of dementia. Hydroxysafflor yellow A (HSYA), a major active chemical component isolated from Carthamus tinctorius L., has been shown to possess neuroprotective actions in various ischemic models in vivo. The present study aimed to investigate the potential protective effect of HSYA against Aß-induced neurotoxicity in cultured rat pheochromocytoma (PC12) cells. The PC12 cells were pretreated with different concentrations (20, 40 and 80 µM) of HSYA for 2 h and then further treated with Aß (20 µM) for 24 h. The results showed that Aß could significantly decrease cell viability, glutathione level, mitochondrial membrane potential and the ratio of Bcl-2/Bax protein expression, while elevate the release of lactate dehydrogenase, the formation of DNA fragmentation, the levels of malondialdehyde and intracellular reactive oxygen species in PC12 cells. However, pretreatment with HSYA could effectively reverse these changes induced by Aß in PC12 cells. Our experimental results demonstrate that HSYA may be a potential neuroprotective agent warranting further development for treatment of AD.


Assuntos
Peptídeos beta-Amiloides/farmacologia , Chalcona/análogos & derivados , Neurônios/efeitos dos fármacos , Quinonas/farmacologia , Animais , Apoptose/efeitos dos fármacos , Chalcona/farmacologia , Potencial da Membrana Mitocondrial/efeitos dos fármacos , Células PC12 , Ratos
9.
Fitoterapia ; 84: 135-9, 2013 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-23160088

RESUMO

Pogostone (PO) is one of the secondary metabolites from Pogostemon cablin (Blanco) Benth. (Lamiaceae), serving as the effective component of the antimicrobial activity. In this study, PO and a series of its analogues were synthesized by the reaction of dehydroacetate and aldehydes in tetrahydrofuran under a nitrogen atmosphere. Their activities against Candida albicans, Gram positive bacteria and Gram negative bacteria were evaluated. The antifungal results demonstrated that PO (MIC ranged from 12 to 97µg/mL against all strains, MFC ranged from 49 to 97µg/mL against all strains) and A3 (MIC ranged from 12 to 49, MFC over 195µg/mL) showed a strong activity against Candida albicans. While A1 (MIC ranged from 49 to 97µg/mL) and A2 (MIC ranged from 24 to 49µg/mL) have only shown effect against Guangzhou clinical isolates, the antibacterial results demonstrated that PO and its analogues showed no effects against the tested bacteria strains. This study suggests that pogostone analogues, with the appropriated structure modification, represented a kind of promising antifungal agents.


Assuntos
Antibacterianos/síntese química , Antibacterianos/farmacologia , Antifúngicos/farmacologia , Lamiaceae/química , Óleos Voláteis/síntese química , Óleos Voláteis/farmacologia , Animais , Bactérias/efeitos dos fármacos , Candida albicans/efeitos dos fármacos , Testes de Sensibilidade Microbiana , Estrutura Molecular
SELEÇÃO DE REFERÊNCIAS
DETALHE DA PESQUISA