Your browser doesn't support javascript.
Mostrar: 20 | 50 | 100
Resultados 1 - 20 de 123
Filtrar
Mais filtros










Base de dados
Intervalo de ano de publicação
1.
Oncol Rep ; 2019 Aug 27.
Artigo em Inglês | MEDLINE | ID: mdl-31485631

RESUMO

Cancer requires aerobic glycolysis to supply the energy required for proliferation. Existing evidence has revealed that blocking glycolysis results in apoptosis of cancer cells. Tanshinone IIA (Tan IIA) is a diterpenoid naphthoquinone found in traditional Chinese medicine, Danshen (Salvia sp.). Tan IIA exhibits potential anticancer activity. However, its effect on cell viability of human cervical cancer cells and its mechanism are unknown. The aim of the present study was to determine the effect of Tan IIA on proliferation and glucose metabolism in cervical cancer cells. Cell viability was measured by MTT assay, apoptosis was determined using flow cytometry and glucose uptake, lactate production, and adenosine triphosphate content were measured to assess glucose metabolism. The expression of apoptosis­associated proteins was detected by western blotting and the antitumor activity of Tan IIA in vivo was evaluated in cervical carcinoma­bearing mice. The results revealed Tan IIA treatment resulted in a considerable reduction in the viability of SiHa cells. Tan IIA decreased the expression of HPV oncogenes E6 and E7, induced apoptosis and also decreased glycolysis by suppressing the activity of the intracellular AKT/mTOR and HIF­1α. In vivo, treatment with Tan IIA resulted in a 72.7% reduction in tumor volume. The present study highlights the potential therapeutic value of Tan IIA, which functions by inducing apoptosis and may be associated with inhibition of glycolysis.

2.
FEMS Yeast Res ; 19(6)2019 Sep 01.
Artigo em Inglês | MEDLINE | ID: mdl-31408151

RESUMO

The construction of a methanol-free expression system of Komagataella phaffii (Pichia pastoris) was attempted by engineering a strong methanol-inducible DAS1 promoter using Citrobacter braakii phytase production as a model case. Constitutive expression of KpTRM1, formerly PRM1-a positive transcription regulator for methanol-utilization (MUT) genes of K. phaffii,was demonstrated to produce phytase without addition of methanol, especially when a DAS1 promoter was used but not an AOX1 promoter. Another positive regulator, Mxr1p, did not have the same effect on the DAS1 promoter, while it was more effective than KpTrmp1 on the AOX1 promoter. Removing a potential upstream repression sequence (URS) and multiplying UAS1DAS1 in the DAS1 promoter significantly enhanced the yield of C. braakii phytase with methanol-feeding, which surpassed the native AOX1 promoter by 80%. However, multiplying UAS1DAS1 did not affect the yield of methanol-free expression by constitutive KpTrm1p. Another important region to enhance the effect of KpTrm1p under a methanol-free condition was identified in the DAS1 promoter, and was termed ESPDAS1. Nevertheless, methanol-free phytase production using an engineered DAS1 promoter outperformed phytase production with the GAP promoter by 25%. Difference in regulation by known transcription factors on the AOX1 promoter and the DAS1 promoter was also illustrated.

3.
J Mater Chem B ; 7(30): 4620-4629, 2019 Jul 31.
Artigo em Inglês | MEDLINE | ID: mdl-31364679

RESUMO

A novel probe, 3-benzyl-2-(N-ethylcarbazole-3-vinyl)-benzothiazolium bromide (L), was synthesized and utilized for the specific detection of cyanide ions in neutral aqueous solution (HEPES buffer, pH 7.4). This probe displays a fast response through visible colorimetric and fluorogenic changes toward CN-, allowing for ratiometric sensing of cyanide in aqueous media. The ratiometric response is due to the breaking of the extensive π-conjugation and the intramolecular charge transfer (ICT) by the nucleophilic conjugation addition of cyanide to L, which is ascertained by NMR and ESI-MS analysis as well as density functional theory (DFT) calculations. The detection limit of CN- (3.39 × 10-7 M) is well below the limit recommended in the World Health Organization (WHO) guidelines for drinking water. Moreover, fluorescence co-localization studies demonstrate that L is a specific mitochondria-targeting fluorescent probe. As far as we are aware, compound L is the first mitochondria-targeting probe for cyanide detection and can be used for sensing CN- in living cells with dual channel ratiometric fluorescence imaging.

4.
Nat Commun ; 10(1): 3876, 2019 Aug 28.
Artigo em Inglês | MEDLINE | ID: mdl-31462636

RESUMO

The salinity gradient between seawater and river water is a clean energy source and an alternative solution for the increasing energy demands. A membrane-based reverse electrodialysis technique is a promising strategy to convert osmotic energy to electricity. To overcome the limits of traditional membranes with low efficiency and high resistance, nanofluidic is an emerging technique to promote osmotic energy harvesting. Here, we engineer a high-performance nanofluidic device with a hybrid membrane composed of a silk nanofibril membrane and an anodic aluminum oxide membrane. The silk nanofibril membrane, as a screening layer with condensed negative surface and nanochannels, dominates the ion transport; the anodic aluminum oxide membrane, as a supporting substrate, offers tunable channels and amphoteric groups. Thus, a nanofluidic membrane with asymmetric geometry and charge polarity is established, showing low resistance, high-performance energy conversion, and long-term stability. The system paves avenues for sustainable power generation, water purification, and desalination.

5.
Angew Chem Int Ed Engl ; 58(36): 12481-12485, 2019 Sep 02.
Artigo em Inglês | MEDLINE | ID: mdl-31317609

RESUMO

A biomimetic conical submicrochannel (tip side ca. 400 nm) with functions of continuously tunable ion rectification and conductance based on thermoresponsive polymer layer-by-layer (LbL) self-assembly is presented. These self-assembled polymers with different layers exhibited a capability to regulate the effective channel diameter, and different ion rectifications/conductance were achieved. By controlling temperature, the conformation and wettability of the assembled polymers were reversibly transformed, thus the ion rectification/conductance could be further adjusted subtly. Owing to the synergistic effect, the ion conductance could be tuned over a wide range spanning three orders of magnitude. Moreover, the proposed system can be applied for on-demand on-off molecule delivery, which was important for disease therapy. This study opens a new door for regulating channel size according to actual demand and sensing big targets with different size with one channel.

6.
Endosc Ultrasound ; 2019 May 09.
Artigo em Inglês | MEDLINE | ID: mdl-31115388

RESUMO

Background and Objectives: Digital image analysis (DIA) has been reported to be a sensitive method to detect pancreatic neoplasms by assessing nuclear DNA content. The aim of the current study was to evaluate the diagnostic performance of DIA for pancreatic malignancy relative to conventional cytology (CC) in patients undergoing EUS-FNA. Patients and Methods: One hundred and forty-two patients with suspected pancreatic malignancy were enrolled prospectively in this study. FNA sample from each patient was evenly divided and assessed by CC and DIA. Findings from histopathology of FNA specimens and a minimum 12-month follow-up were used as the standard. Results: Total 102 out of 142 patients were determined as pancreatic cancer (PC) in this study. The overall sensitivity, specificity, and accuracy of CC were 75%, 85%, and 78%, whereas the sensitivity, specificity, and accuracy of DIA were 84%, 80%, and 83%, respectively. A combination of CC and DIA (CC/DIA) showed greater sensitivity than CC alone (92% vs. 75%, P < 0.01), but the specificity was reduced from 85% to 70% (P = 0.03). Conclusions: In this study, we demonstrated that DIA provided comparable diagnostic performance to CC in detecting PC. This objective diagnostic method, DIA, emerged as an important supplementary tool to endoscopic biopsy and cytology for diagnosing patients undergoing EUS-FNA with suspected pancreatic malignancy.

7.
J Virol ; 93(15)2019 Aug 01.
Artigo em Inglês | MEDLINE | ID: mdl-31118252

RESUMO

Human noroviruses (huNoVs) recognize histo-blood group antigens (HBGAs) as host susceptibility factors. GII.13 and GII.21 huNoVs form a unique genetic lineage that emerged from mainstream GII NoVs via development of a new, nonconventional glycan binding site (GBS) that binds Lea antigen. This previous finding raised the question of whether the new GII.13/21 GBS really has such a narrow glycan binding spectrum. In this study, we provide solid phenotypic and structural evidence indicating that this new GBS recognizes a group of glycans with a common terminal ß-galactose (ß-Gal). First, we found that P domain proteins of GII.13/21 huNoVs circulating at different times bound three glycans sharing a common terminal ß-Gal, including Lec, lactose, and mucin core 2. Second, we solved the crystal structures of the GII.13 P dimers in complex with Lec and mucin core 2, which showed that ß-Gal is the major binding saccharide. Third, nonfat milk and lactose blocked the GII.13/21 P domain-glycan binding, which may explain the low prevalence of GII.13/21 viruses. Our data provide new insight into the host interactions and epidemiology of huNoVs, which would help in the control and prevention of NoV-associated diseases.IMPORTANCE Evidence from both phenotypic binding assay and structural study support the observed interactions of human noroviruses (huNoVs) with histo-blood group antigens (HBGAs) as receptors or attachment factors, affecting their host susceptibility. GII.13 and GII.21 genotypes form a unique genetic lineage that differs from the mainstream GII huNoVs in their unconventional glycan binding site. Unlike the previous findings that GII.13/21 genotypes recognize only Lea antigen, we found in this study that they can interact with a group of glycans with a common terminal ß-Gal, including Lec, lactose, and mucin core 2. However, this wide glycan binding spectrum in a unique binding mode of the GII.13/21 huNoVs appears not to increase their prevalence, probably due to the existence of decoy glycan receptors in human gastrointestinal tract limiting their infection. Our findings shed light on the host interaction and epidemiology of huNoVs, which would impact the strategy of huNoV control and prevention.

8.
Asia Pac J Clin Oncol ; 15(5): e162-e166, 2019 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-30990963

RESUMO

AIM: This study aimed to investigate the correlation between the expression of A-kinase anchor protein95 (AKAP95), p-retinoblastoma (phosphorylated Rb, p-Rb), cyclin D2, cyclin D3 and cyclin E2 in esophageal cancer tissues and clinicopathological indexes. METHOD: The protein expression levels of AKAP95, p-Rb, cyclin D2/3 and cyclin E2 in 40 esophageal cancer tissues were detected using immunohistochemistry, and the correlation between them was analyzed. RESULT: The percentage of p-Rb (Ser780)-, cyclin D2-, cyclin D3- and cyclin E2-positive samples was 62.50%, 70.00%, 67.50% and 60.00%, respectively. Also, the positive expression did not correlate with the histological type, histological differentiation or lymph node metastasis. The expression of AKAP95 and p-Rb (Ser780), p-Rb (Ser780) and cyclin D2 and p-Rb (Ser780) and cyclin D3 in esophageal cancer tissues was found to be correlated (P < 0.05). CONCLUSIONS: The expression of AKAP95 and p-Rb (Ser780), p-Rb(Ser780) and cyclin D2, and p-Rb (Ser780) and cyclin D3 in esophageal cancer tissue was correlated, suggesting that these proteins might play a synergistic role in cell-cycle progression. Cyclin D2/D3 and p-Rb (Ser780) were correlated whereas cyclin E2 and p-Rb (Ser780) were not, suggesting that p-Rb (Ser780) might be highly expressed and the Ser780 site of Rb protein might be phosphorylated in the early stage of the G1 phase. Ser780 was the site in the primary phosphorylation stage of several phosphorylation sites during stepwise phosphorylation (from primary to high phosphorylation).

9.
Anal Cell Pathol (Amst) ; 2019: 7145198, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-30809467

RESUMO

Objective: To assess the expression levels of exchange protein 1 directly activated by cAMP (Epac1) and phosphodiesterase 4 (PDE4) in rectal carcinoma, and their associations with clinicopathological indexes. In addition, the associations of PDE4 and Epac1 with A-kinase anchor protein 95, connexin 43, cyclin D1, and cyclin E1 were evaluated. Methods: The PV-9000 two-step immunohistochemistry method was used to determine protein expression in 44 rectal carcinoma tissue samples and 16 paracarcinoma tissue specimens. Results: The positive rate of PDE4 protein expression in rectal carcinoma tissues was higher than that of paracarcinoma tissues (59.09% vs. 12.5%, P < 0.05). Similar findings were obtained for Epac1 (55% vs. 6.25%, P < 0.05). No significant associations of PDE4 and Epac1 with degree of differentiation, histological type, and lymph node metastasis were found in rectal carcinoma (P > 0.05). Correlations between PDE4 and Epac1, PDE4 and Cx43, PDE4 and cyclin E1, and Epac1 and Cx43 were observed (all P < 0.05). There was no correlation between the other protein pairs examined (P > 0.05). Conclusion: PDE4 and Epac1 expression levels are increased in rectal carcinoma tissues, suggesting that the two proteins may be involved in the development of this malignancy. Meanwhile, correlations between PDE4 and Epac1, PDE4 and Cx43, PDE4 and cyclin E1, and Epac1 and Cx43 suggested synergistic effects of these proteins in promoting rectal carcinoma.


Assuntos
Carcinoma/metabolismo , AMP Cíclico/metabolismo , Nucleotídeo Cíclico Fosfodiesterase do Tipo 4/metabolismo , Fatores de Troca do Nucleotídeo Guanina/metabolismo , Neoplasias Retais/metabolismo , Proteínas de Ancoragem à Quinase A/metabolismo , Adulto , Idoso , Conexina 43/metabolismo , Ciclina D1/metabolismo , Ciclina E/metabolismo , Feminino , Humanos , Técnicas In Vitro , Masculino , Pessoa de Meia-Idade , Proteínas Oncogênicas/metabolismo , Transdução de Sinais/genética , Transdução de Sinais/fisiologia
10.
Spectrochim Acta A Mol Biomol Spectrosc ; 208: 131-139, 2019 Feb 05.
Artigo em Inglês | MEDLINE | ID: mdl-30308397

RESUMO

A novel fluorescent probe, phenolphthalein­dialdehyde­(2­pyridyl) hydrazone (L), for sequentially detecting Al3+ and F- in almost 100% aqueous medium was successfully designed and synthesized. The probe offers two binding pockets for Al3+ to form a 1: 2 ligand/metal complex, leading to a significant fluorescence enhancement at 465 nm. Further, the in-situ formed L-Al complex acts as a secondary fluorescent chemosensor for F- by quenching the fluorescence of the complex with high selectivity. The detection limit for Al3+ and F- sensing is 2.28 nM and 0.13 µM, respectively, which are far below the World Health Organization (WHO) acceptable limits (7.41 µM for Al3+ ion and 79 µM for F-) in drinking water. The probe L was successfully applied to the detection of Al3+ and F- in cells using fluorescence microscopy.


Assuntos
Alumínio/análise , Corantes Fluorescentes/química , Fluoretos/análise , Fenolftaleína/química , Água/química , Ânions , Linhagem Celular Tumoral , Sobrevivência Celular , Corantes Fluorescentes/síntese química , Humanos , Concentração de Íons de Hidrogênio , Espectroscopia de Prótons por Ressonância Magnética , Espectrometria de Fluorescência , Espectrofotometria Ultravioleta , Espectroscopia de Infravermelho com Transformada de Fourier
12.
J Am Chem Soc ; 140(47): 16048-16052, 2018 Nov 28.
Artigo em Inglês | MEDLINE | ID: mdl-30372056

RESUMO

In nature, biological machines can perform sophisticated and subtle functions to maintain the metabolism of organisms. Inspired from these gorgeous works of nature, scientists have developed various artificial molecular motors and machines. However, selective transport of biomolecules across membrane has remained a great challenge. Here, we establish an ATP transport system by assembling photocontrolled DNA nanomachines into the artificial nanochannels. With alternant light irradiation, these ATP transport lines can selectively shepherd cargoes across the polymer membrane. These findings point to new opportunities for manipulating and improving the mass transportation and separation with light-controlled biomolecular motors, and can be used for other molecules and ions transmembrane transport powered by light.

13.
Artigo em Inglês | MEDLINE | ID: mdl-30358031

RESUMO

In nature, biological machines and motors can selectively transport cargoes across the lipid membranes to efficiently perform various physiological functions via ion channels or ion pumps. It is interesting and challengeable to develop artificial motors and machines of nanodimensions to controllably regulate mass transport in compartmentalized systems. In this work, we show a system of artificial molecular motors that uses light energy to perform transmembrane molecule transport through synthetical nanochannels. After functionalizing the polymer nanochannels with azobenzene derivatives, these nanomachines exhibit autonomous selective transport behavior over a long distance upon simultaneous irradiation with UV (365 nm) and visible (430 nm) light. With new strategies or suitable materials for directed molecular movement, such device can be regarded as a precursor of artificial light-driven molecular pumps.

14.
Artigo em Inglês | MEDLINE | ID: mdl-30336616

RESUMO

Long-term exposure to high levels of heavy metals can lead to a variety of diseases. In recent years, researchers have paid more attention to mining and smelting areas, industrial areas, and so forth, but they have neglected to report on high geological background areas where heavy metal levels are higher than China's soil environmental quality standard (GB 15618-2018). In our study, an investigation of heavy metals in paddy soil and rice in the high background area of Guizhou Province was carried out, and the factors affecting the absorption and utilization of heavy metals in rice were discussed. A total of 52 paddy soil and rice samples throughout the high geological background of Guizhou, China, were collected, and concentration(s) of arsenic, cadmium, copper, lead, and zinc were analyzed. The arithmetic mean values of paddy soil heavy metals were 19.7 ± 17.1, 0.577 ± 0.690, 40.5 ± 32.8, 35.5 ± 32.0, and 135 ± 128 mg kg-1 for arsenic, cadmium, copper, lead, and zinc, respectively. Most of the heavy metals' contents in the soil were above the soil standard value. The highest content of cadmium was 15.5 times that of the soil standard value. The concentration(s) of arsenic, cadmium, copper, lead, and zinc in rice were 0.09 ± 0.03, 0.01 ± 0.01, 1.57 ± 0.69, 0.002 ± 0.003, and 11.56 ± 2.61 mg kg-1, respectively, which are all lower than those specified by Chinese food safety standards (GB 2762-2017). The results and discussion show that the bioavailability, pH, and soil organic matter are important factors that affect the absorption of heavy metals by rice. According to the consumption of rice in Guizhou Province, the risk of eating rice was considered. The results revealed that the hazard quotient is ranked in the order of copper > zinc > cadmium > arsenic > lead, and there is little risk of eating rice in the high geological background area of Guizhou Province. These findings provide impetus for the revision and improvement of this Chinese soil environmental quality standard.

15.
Oncogene ; 37(45): 6010, 2018 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-30108331

RESUMO

Since the online publication of the above article, the authors have noted errors in the affiliations. The authors apologise for any inconvenience caused by this error. The html and online pdf versions have now been rectified and carry the corrected paper.

16.
Curr Mol Pharmacol ; 11(4): 316-325, 2018.
Artigo em Inglês | MEDLINE | ID: mdl-30101722

RESUMO

OBJECTIVE: To explore the mechanism by which lobaplatin, as monotherapy and in combination with paclitaxel, inhibits the proliferation of human gastric cancer SGC-7901 cells. METHODS: After treatment, the MTT assay was used to assess cell viability; cell cycle distribution was evaluated flow-cytometrically. Western blot was used to quantitate cyclin D1, E1, B1, and Cdk2/4 protein levels. RESULTS: Lobaplatin and paclitaxel inhibited SGC-7901 cell growth in a concentration and timedependent manner, with IC25 values at 48h of 1.97±0.17µg/ml and 1.98±0.19 ng/ml, respectively. Interestingly, both drugs synergistically inhibited SGC-7901 cells (combination index [CI]<0.95). Lobaplatin did not affect cyclin D1 and CDK4 protein expression, while cyclin E1 and CDK2 levels were significantly increased, with cyclin B1 amounts markedly decreased (p<0.05). More S phase cells were observed after lobaplatin treatment compared with controls (60.03±1.25 vs. 18.69±0.96%; p<0.05). After treatment with paclitaxel, cyclin D1 and CDK4 protein levels were similar to control values; meanwhile, cylinE1 and CDK2 protein amounts were reduced, with increased cyclin B1 levels, compared with control values (p<0.05). More G2/M cells were obtained after treatment with paclitaxel compared with control values (74.54±0.92 vs. 18.62±0.44% (p<0.05). Lobaplatin and paclitaxel combination did not affect cyclin D1 and CDK4 protein levels (p>0.05); meanwhile, cyclin E1 and CDK2 levels were increased, with reduced cyclin B1 amounts, compared with control values (p<0.05). Notably, more S (43.23±0.81 vs. 22.32±0.86%) and G2/M (31.22±0.96 vs. 25.81±2.08%) phase cells were obtained after combined treatment compared with control values. CONCLUSION: Lobaplatin and paclitaxel synergistically inhibit SGC-7901 cells.

17.
ACS Appl Mater Interfaces ; 10(36): 30852-30859, 2018 Sep 12.
Artigo em Inglês | MEDLINE | ID: mdl-30124286

RESUMO

Inspired by the delicate functions of living organisms to transport or transform nitrite ions (NO2-), a bioinspired smart nanochannel that can realize harmless conversion of NO2- into N2 is developed by immobilizing a NO2--responsive functional molecule, p-phenylenediamine, onto a single conical polyethylene terephthalate nanochannel. Subsequently, the aromatic primary amine groups could be triggered to transform into a phenyldiazonium molecule based on the acid-activated NO2--binding process. The nanochannel exhibits specific selectivity and highly ultratrace recognition of NO2-. Fascinatingly, the transformed phenyldiazonium molecules could be triggered to generate phenol groups and release N2 by ultraviolet light activation, achieving NO2- harmless conversion. This system could provide inspiration to construct artificial nanofluidic devices for ion-sensing and nitrogen cycle fields.

18.
J Phys Condens Matter ; 30(33): 335501, 2018 Aug 22.
Artigo em Inglês | MEDLINE | ID: mdl-29995644

RESUMO

The structural, electronic and magnetic properties of dual Cr atoms doped germanium anionic clusters, [Formula: see text] (n = 3-14), have been investigated by using photoelectron spectroscopy in combination with density-functional theory calculations. The low-lying structures of [Formula: see text] are determined by DFT based genetic algorithm optimization. For [Formula: see text] with n ⩽ 8, the structures are bipyramid-based geometries, while [Formula: see text] cluster has an opening cage-like structure, and the half-encapsulated structure is gradually covered by the additional Ge atoms to form closed-cage configuration with one Cr atom interior for n = 10 to 14. Meanwhile, the two Cr atoms in [Formula: see text] clusters tend to form a Cr-Cr bond rather than be separated. Interestingly, the magnetic moment of all the anionic clusters considered is 1 µ B. Almost all clusters exhibit antiferromagnetic Cr-Cr coupling, except for two clusters, [Formula: see text] and [Formula: see text]. To our knowledge, the [Formula: see text] cluster is the first kind of transition-metal doped semiconductor clusters that exhibit relatively stable antiferromagnetism within a wide size range. The experimental/theoretical results suggest high potential to modify the magnetic behavior of semiconductor clusters through introducing different transition-metal dopant atoms.

19.
Oncogene ; 37(45): 5982-5996, 2018 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-29985481

RESUMO

Long non-coding RNAs (lncRNAs) are implicated to be involved in the pathogenesis of many cancers. Herein we report on our discovery of a novel lncRNA, ZFPM2 antisense RNA 1 (ZFPM2-AS1), and its critical role in gastric carcinogenesis. ZFPM2-AS1 expression in gastric cancer specimens was analyzed using Gene Expression Omnibus data set and validated in 73 paired gastric tumor and normal adjacent gastric tissue specimens using qRT-PCR. The effect of ZFPM2-AS1 expression on proliferation and apoptosis in gastric cancer cells was assessed by altering its expression in vitro and in vivo. Mechanistic investigation was carried out using cell and molecular biological approaches. ZFPM2-AS1 expression was higher in gastric tumors than in normal gastric tissue. Also, increased ZFPM2-AS1 expression in gastric cancer specimens was associated with tumor size, depth of tumor invasion, differentiation grade, and TNM stage. High ZFPM2-AS1 expression predicted markedly reduced overall and disease-free survival in gastric cancer patients. Functional experiments demonstrated that ZFPM2-AS1 expression promoted proliferation and suppressed apoptosis of gastric cancer cells in vitro and promoted tumor growth in vivo. This effect is associated with attenuated nuclear translocation of p53. Mechanistic experiments demonstrated that tumor-activated ZFPM2-AS1 could bind to and protect the degradation of macrophage migration inhibitory factor (MIF), a potent destabilizer of p53. Knockdown of MIF expression diminished ZFPM2-AS1's impact on p53 expression in gastric cancer cells. Our findings demonstrated that ZFPM2-AS1 regulates gastric cancer progression and revealed a novel ZFPM2-AS1/MIF/p53 signaling axis, shedding light on the molecular mechanisms underlying the tumorigenicity of certain malignant gastric cells.

20.
Molecules ; 23(6)2018 Jun 15.
Artigo em Inglês | MEDLINE | ID: mdl-29914082

RESUMO

Botrytis cinerea is the pathogen of gray mold disease affecting a wide range of plant hosts, with consequential economic losses worldwide. The increased frequency of fungicide resistance of the pathogen challenges its disease management, and thus the development of alternative control strategies are urgently required. In this study, we showed excellent synergistic interactions between resveratrol and pyrimethanil. Significant synergistic values were recorded by the two-drug combination on the suppression of mycelial growth and conidia germination of B. cinerea. The combination of resveratrol and pyrimethanil caused malformation of mycelia. Moreover, the inoculation assay was conducted on table grape and consistent synergistic suppression of the two-drug combination was found in vivo. Our findings first revealed that the combination of resveratrol and pyrimethanil has synergistic effects against resistant B. cinerea and support the potential use of resveratrol as a promising adjuvant on the control of gray mold.


Assuntos
Botrytis/efeitos dos fármacos , Doenças das Plantas/prevenção & controle , Pirimidinas/farmacologia , Estilbenos/farmacologia , Vitis/microbiologia , Farmacorresistência Fúngica/efeitos dos fármacos , Sinergismo Farmacológico , Micélio/efeitos dos fármacos , Doenças das Plantas/microbiologia , Resveratrol , Esporos Fúngicos/efeitos dos fármacos
SELEÇÃO DE REFERÊNCIAS
DETALHE DA PESQUISA