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1.
Anal Chim Acta ; 1044: 29-65, 2018 Dec 31.
Artigo em Inglês | MEDLINE | ID: mdl-30442405

RESUMO

The pivotal role of microfluidic technology in life science and biomedical research is now widely recognized. Indeed, microfluidics as a research tool is unparalleled in terms of its biocompatibility, robustness, efficient reagent consumption, and controlled fluidic, surface, and structure environments. The controlled environments are essential in assessing the complex behavior of cells in response to microenvironmental cues. The strengths of microfluidics also reside in its amenability to integration with other analytical platforms and its capacity for miniaturization, parallelization and automation of biochemical assays. Following previous review on the applications of microfluidic devices for cell-based assays in 2006, we have monitored the progress in the field and summarized the advances in microfluidic technology from 2007 to 2017, with a focus on microfluidics development for applications in cell manipulation, cell capture and detection, and cell treatment and analysis. Moreover, we highlighted novel commercial microfluidic products for biomedical and clinical purposes that were introduced in the review period. Thus, this review provides a comprehensive source for recent developments in microfluidics and presents a snapshot of its remarkable contribution towards basic biomedical research and clinical science. We recognize that although enormous amounts of evidence have reinforced the promise of microfluidic technology across diverse applications, much remains to be done to realize its full potential in mainstream biomedical science and clinical practice.


Assuntos
Neoplasias da Mama/patologia , Células-Tronco Mesenquimais/citologia , Técnicas Analíticas Microfluídicas , Linhagem Celular , Separação Celular , Feminino , Humanos , Técnicas Analíticas Microfluídicas/instrumentação
2.
Drug Des Devel Ther ; 8: 475-83, 2014.
Artigo em Inglês | MEDLINE | ID: mdl-24872678

RESUMO

Periplocin, an active and toxic component of the traditional Chinese herbal medicine Periploca sepium Bge, is a cardiac glycoside compound that has been implicated in various clinical accidents. This study investigated the role of transporters in the intestinal absorption and biliary excretion of periplocin, as well as the possible metabolic mechanism of periplocin in liver S9. In a bidirectional transport assay using Madin-Darby canine kidney (MDCK) and MDCK multidrug-resistance protein (MRP)-1 cell monolayers, both in situ intestinal and liver-perfusion models were used to evaluate the role of efflux and uptake transporters on the absorption and biliary excretion of periplocin. In addition, in vitro metabolism of periplocin was investigated by incubating with human/rat liver S9 homogenate fractions to evaluate its metabolic mechanisms in liver metabolic enzymes. The results showed that P-glycoprotein (P-gp) was involved in the intestinal absorption of periplocin, whereas MRP2 and breast cancer-resistance protein were not. The efflux function of P-gp may be partly responsible for the low permeability and bioavailability of periplocin. Moreover, both inhibitors of P-gp and organic anion-transporting polypeptides (OATPs) increased periplocin biliary excretion. No obvious indications of metabolism were observed in the in vitro incubation system, which suggests that periplocin did not interact with the hepatic drug metabolic enzymes. The results of this study showed that the efflux and uptake transporters P-gp and OATPs were involved in the absorption and biliary excretion of periplocin, which may partially account for its low permeability and bioavailability. As a toxic compound, potential drug-herb/herb-herb interactions based on OATPs and P-gp should be taken into account when using P. sepium Bge in the clinic.


Assuntos
Membro 1 da Subfamília B de Cassetes de Ligação de ATP/fisiologia , Interações Ervas-Drogas , Transportadores de Ânions Orgânicos/fisiologia , Saponinas/farmacocinética , Animais , Bile/metabolismo , Transporte Biológico , Cães , Interações de Medicamentos , Humanos , Absorção Intestinal , Células Madin Darby de Rim Canino , Masculino , Ratos , Ratos Sprague-Dawley
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