Your browser doesn't support javascript.
Mostrar: 20 | 50 | 100
Resultados 1 - 20 de 172
Filtrar
Mais filtros










Intervalo de ano de publicação
1.
Int J Biol Macromol ; 146: 1000-1008, 2020 Mar 01.
Artigo em Inglês | MEDLINE | ID: mdl-31726146

RESUMO

A 61.3 kDa Phenol hydroxylase (PheA) was purified and characterized from Pseudomonas sp. KZNSA (PKZNSA). Cell free extract of the isolate grown in mineral salt medium supplemented with 600 ppm phenol showed 21.58 U/mL of PheA activity with a specific activity of 7.67 U/mg of protein. The enzyme was purified to 1.6-fold with a total yield of 33.6%. The purified PheA was optimally active at pH 8 and temperature 30 °C, with ≈95% stability at pH 7.5 and temperature 30 °C after 2 h. The Lineweaver-Burk plot showed the vmax and Km values of 4.04 µM/min and 4.03 µM, respectively, for the substrate phenol. The ES-MS data generated from the tryptic digested fragments of pure protein and PCR amplification of a ≈600 bp gene from genomic DNA of PKZNSA lead to the determination of complete amino acid and nucleotide sequence of PheA. Bioinformatics tools and homology modelling studies indicated that PheA from PKZNSA is likely a probable protein kinase UbiB (2-octaprenylphenol hydroxylase) involving Lys and Asp at positions 153 and 288 for binding and active site, respectively. Characterization and optimization of PheA activity may be useful for a better understanding of 2,4-dichlorophenol degradation by this organism and for potential industrial application of the enzyme.

3.
Indian J Otolaryngol Head Neck Surg ; 71(Suppl 2): 1535-1537, 2019 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-31750212

RESUMO

To study the role of Otoacoustic emission test in neonatal screening at tertiary center. The study was conducted in the ENT department of LN medical college and JK hospital Bhopal (M.P) as a territory center between 2015 and 2017 were 1250 newborns were screened with Otoacoustic emission test with portable Interacoustics OAE DK-5610 assens an routine screening investigation. In our study a multistep screening with OAE was done, 1250 newborns (659 boys and 591 girls) were assessed. In our study we have found that OAE has 66.7% sensitivity and 98.8% specificity in diagnosis of neonatal hearing impairment. Its positive and negative predictive value was 33.3% and 99.7% respectively. It is essential to examine the generalized development of newborns by identifying hearing loss at birth and providing a screening test for assessment of them. 1250 newborns were assessed we compared the results of OAE (screening test) with the results of ABR (diagnostic test) and found sensitivity of OAE to be 66.7% and its specificity 98.8%. We conclude from these results that OAE is a good screening test for hearing loss of neonates, but the results must be confirmed with BERA test.

4.
Rev Sci Instrum ; 90(8): 083306, 2019 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-31472612

RESUMO

The performance of a newly built omega type electrostatic analyzer designed to act as an in-line charge-state purification system for ions in the kiloelectronvolt energy range is reported. The analyzer consists of a set of four consecutive electrostatic 140° concentric cylindrical electrodes enclosed by Matsuda electrodes. This setup was recently tested and validated using O5+, Ar9+, and Xe20+ ion beams at an energy of 14 qkeV at the ARIBE facility. A resolving power of 10.5 and a transmission of 100% of the desired charge state are measured allowing a good purification of incoming ion beams with charge states up to 10+ and a fairly good purification for charge states at least up to 20+. In comparison with other in-line solutions such as the Wien filter, our system has the advantage of being purely electrostatic and therefore lacking common drawbacks as, for example, hysteresis.

5.
Mini Rev Med Chem ; 2019 Sep 04.
Artigo em Inglês | MEDLINE | ID: mdl-31483229

RESUMO

Comparative molecular field analysis (CoMFA) of 27 analogues of 2-((pyridin-3-yloxy)methyl)piperazine derivatives was carried out using software Tripos SYBYL X. Optimal r2 (0.854) and q2 (0.541) values were obtained for developed 3D QSAR model. The contour plots obtained from CoMFA analysis have shown 13.84% steric contribution and 66.14% electrostatic contribution towards anti-inflammatory activity. The homology model of the receptor protein, α7 nicotinic acetylcholine, was generated in SWISS MODELLER using auto template mode and was analysed for the quality using Procheck, QMEAN Z-score, Anolea and GROMOS plots. The QMEAN score for the model was observed to be -3.862. The generated model of alpha 7 nicotinic acetylcholine receptor was used for docking study of 27 piperazine analogues using Auto-Dock 4.2.5.1. The dock score obtained from docking analysis was then correlated with experimental pIC50 values for in-silico validation of the developed CoMFA model and a good correlation was obtained with correlation coefficient (r2) value of -0.7378. The present investigation suggests an optimal 3D-QSAR with CoMFA model for further evaluating new chemical entities based on piperazine skeleton.

7.
Bioinformation ; 15(4): 246-254, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31285641

RESUMO

Wnt-Fzd signalling plays vital role in different physiological pathways including embryonic development and supposed to be probable target of many teratogens. The present study was done to investigate the role of human Wnt5b interaction with different isoforms of human Fzds and also the molecular interactions of their complexes with selected known teratogens [Carbamazepine (CBZ), Retinoic acid (RA), Valproic acid (VPA), Aminopterin (AMP) and Phenytoin (PHY)] using Niclosamide (NLM) as standard. The models of hWnt5b and hFzd isoforms, whose solved crystal structures were unavailable, were generated using homology modeling and hWnt5b was subjected to protein-protein docking studies against different isoforms of hFzd. The macromolecular docking studies of hWnt5b-hFzds complexes revealed that hWnt5b had highest binding affinity with hFzd8 and lowest with hFzd1, respectively. The Cysteine rich domain (CRD) of hFzds docked against hWnt5b into a palm shaped opening or near the largest binding pocket as in hWnt5b-hFzd6. The possible role of Wnt-Fzd interactions in developmental toxicity due to selected teratogens were also investigated using molecular docking studies which showed that Retinoic Acid possessed the maximum binding affinity with binding energy of for hWnt5b-hFzd8 complex while VPA was observed to have lowest binding affinity towards all the studied hWnt5b-hFzd complexes.

8.
Int J Biol Macromol ; 137: 87-94, 2019 Sep 15.
Artigo em Inglês | MEDLINE | ID: mdl-31254580

RESUMO

Mishmi takin (Budorcas taxicolor taxicolor) is an endangered and least studies species. We sequenced and annotated a 16,584 bp long complete mitogenome of Mishmi takin (B. t. taxicolor). It was encoded by 37 genes: 13 Protein-Coding Genes (PCGs), two ribosomal RNA genes, 22 transfer RNA, and non-coding control region. All tRNA genes show a typical secondary cloverleaf structure, except tRNAser(AGY) in which dihydrouridine arm did not form a stable structure. We observed 82 bp nucleotide deletions in the control region of Mishmi takin. The overall analysis of 13 PCGs, two rRNA and 22 tRNA of Mishmi takin indicates its close relationship with the B. t. tibetana than the B. t. bedfordi. The overall genetic similarity indicated that all takin species is closely related to the P. nayaur than the C. ibex and Ovibos moschatus. This study provides a useful resource for further studies on conservation and population genetics of Budorcas.


Assuntos
Artiodáctilos/genética , Genoma Mitocondrial/genética , Análise de Sequência , Animais , Artiodáctilos/classificação , Sequência de Bases , Genômica , Anotação de Sequência Molecular , Filogenia , RNA Ribossômico/genética , RNA de Transferência/genética , Especificidade da Espécie
9.
Bioinformation ; 15(5): 307-314, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31249432

RESUMO

WNT proteins are involved from initial of neural tissue specification to the end of cell fate determination and organ development. The present work was carried out to understand the involvement of different WNT isoforms (WNT3a, WNT5a and WNT7b) in neural development. A total of 718, 546 and 1004 PPIs for WNT3a, WNT5a and WNT7b respectively, were predicted by STRING database with confidence score more than 0.400. A network carrying all the selected PPIs of targeted proteins was constructed by using Cytoscape by assigning source node, target node and combined score as edge attribute. A total 2268 interactions of WNT3a, WNT5a and WNT7b were predicted to be involved in multiple signaling pathways and developmental processes. 43 of 2268 PPIs were refined after analyzing role of targeted proteins specifically in brain and neural development. WNT3a, WNT5a and WNT7a were predicted to be interacting with 18, 17 and 11 proteins, respectively, with average node degree score of 1.89, 2.12 and 1.82 respectively. The CytoHubba algorithm identified WNT3a, WNT5a, and WNT7b as hub proteins in neural development ranked on the basis of EPC (Edge Percolated Component) score of 9.352, 9.258 and 8.387.

10.
Indian J Ophthalmol ; 67(7): 1048-1053, 2019 07.
Artigo em Inglês | MEDLINE | ID: mdl-31238406

RESUMO

Purpose: The aim of the study is to identify risk factors, clinical characteristics, causative fungi, and treatment outcome of dematiaceous fungal keratitis in North India. Methods: Consecutive cases of culture-proven dematiaceous fungal keratitis between January 2012 and June 2017 were retrieved from the medical record department. Risk factors, clinical signs, and outcome were registered. Results: Eighty-three patients were included. Identified dematiaceous fungal organism were Curvularia sp. (n = 55/83; 66.3%), Alternaria sp. (n = 12/83; 14.5%), Ulocladium sp. (n = 5/83; 6%), Bipolaris sp. (n = 5/83; 6.1%), Scedosporium sp. (n = 3/83; 3.6%), Acremonium sp. (n = 2/83; 2.4%), and Epicoccum sp. (n = 1/83; 1.2%). Male preponderance was reported. The most common predisposing factor was corneal trauma (67.4%). In cases associated with corneal trauma due to vegetative matter, sugarcane was the most common cause. In all, 89% of the patients were more than 30 years of age. The median infiltrate size was 8 mm2. The median time of antifungal therapy was 4.2 weeks (interquartile range [IQR]: 1-25 weeks). Complications were seen in 14 (n = 14/65; 21.5%) patients. Complete resolution of dematiaceous fungal keratitis was present in 27 (n = 27/65; 41.5%) eyes. Conclusion: Curvularia sp. and Alternaria sp. were the predominant pathogenic genera causing dematiaceous fungal keratitis. Among the causative fungi, infections due to Scedosporium sp. were associated with the worst outcomes. Ulocladium sp. and Epicoccum sp. were also identified. Both the species are not reported previously as a causal organism of dematiaceous fungal keratitis from North India.


Assuntos
Infecções Oculares Fúngicas/microbiologia , Ceratite/microbiologia , Fungos Mitospóricos/isolamento & purificação , Micoses/microbiologia , Adulto , Córnea/microbiologia , Córnea/patologia , Infecções Oculares Fúngicas/diagnóstico , Infecções Oculares Fúngicas/epidemiologia , Feminino , Seguimentos , Humanos , Incidência , Índia/epidemiologia , Ceratite/diagnóstico , Ceratite/epidemiologia , Masculino , Pessoa de Meia-Idade , Micoses/diagnóstico , Micoses/epidemiologia , Estudos Retrospectivos , Fatores de Risco , Lâmpada de Fenda
11.
ACS Appl Mater Interfaces ; 11(15): 14101-14109, 2019 Apr 17.
Artigo em Inglês | MEDLINE | ID: mdl-30919631

RESUMO

Room-temperature sodium-sulfur (RT Na-S) battery cathodes suffer from poor conductivity, rapid dissolution of intermediate products, and potentially destructive volume change during cycling. The optimal way to minimize these problems could be a construction of a nanocomposite cathode scaffold combining different components selected for their particular functions. Here, we have combined the excellent electronic conductivity of reduced graphene oxide, polysulfide adsorption ability of the ultrafine manganese oxide nanocrystals, rapid ion/electron dissemination efficiency of nanosized sulfur, and outstanding mechanical stiffness and good electrical conductivity of Na alginate/polyaniline hybrid binder in a single electrode heterostructure. At 0.2 A g-1, an RT Na-S battery containing the freestanding cathode delivers an initial specific cap acity of 631 mA h g-1. By delivering a nominal discharge voltage of 1.81 V, our Na-S batteries bestow a high specific energy of 737 W h kg-1 at the 2nd cycle and 660 W h kg-1 was retained after 50 cycles. The effect of the amount of electrolyte additive is also well demonstrated in this study. The electrode fabrication process provides a new approach to tailor the design and preparation of effective cathodes for the room-temperature sodium-sulfur batteries.

12.
ISA Trans ; 90: 189-201, 2019 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-30755311

RESUMO

Novel ways of regulating the speed of a hydraulic drive through controlled operation of the pump either by loading/unloading the UPRV (unloading pressure relief valve) or by switching on/off of the prime mover driving the pump have been presented in this paper. The UPRV is used to operate the pump with a minimum load by diverting the pump delivery to the tank at low pressure. The energy efficiency of the said two methods adopted for controlling the hydro-motor speed is also compared. In this respect, the MATLAB®/Simulink model of the system is made and validated experimentally. The static and dynamic performances of the system are investigated using the validated model. The results show that the UPRV controlled system demonstrates better dynamic performance and controllability in terms of decreased fluctuations in hydro-motor speed for the small load torque demand. However, by comparing the energy efficiency of the hydraulic system for the two control strategies, it is found that with the increase in the machine torque demand, the intermittent operation of the pump unit may be more preferable with respect to the amount of energy saved for the complete duty cycle of the system.

13.
Acta Parasitol ; 64(1): 57-62, 2019 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-30645735

RESUMO

AIMS: Canine demodicosis is a parasitic condition affecting the skin of dogs. The present study was designed to characterize chitin synthase gene of Demodex canis. The molecular technique was used for better understanding of this gene. METHODS: A total of 75 dogs which are reared as pets with or without showing any skin lesions were examined during the study period. Skin scrapings were examined by indirect method using 10% potassium hydroxide solution under 10 × microscope. DNA samples were extracted from positive skin samples and were subjected to PCR for molecular identification. RESULTS: A total of 25 dogs irrespective of age, sex, breed or coat showed positive result for D. canis. The PCR revealed a single amplified product of 339 bp length which exactly matched with D. canis. The chitin synthase gene was amplified by PCR, subsequently cloned, sequenced, and compared with available data in GenBank for the particular gene of D. canis. Only one single nucleotide polymorphism (SNP) was noticed at 231 position of the chitin synthase gene sequence when compared to other isolates. CONCLUSION: The molecular technique confirms with the morphological identity of D. canis. This report signifies the value of peculiar tool to identify 'follicular mite' even from apparently healthy skin.


Assuntos
Ácaros e Carrapatos/enzimologia , Quitina Sintase/genética , Animais , Clonagem Molecular , DNA/genética , DNA/isolamento & purificação , Doenças do Cão/parasitologia , Cães , Índia , Infestações por Ácaros/parasitologia , Infestações por Ácaros/veterinária , Parasitologia/métodos , Reação em Cadeia da Polimerase , Polimorfismo de Nucleotídeo Único , Análise de Sequência de DNA , Pele/parasitologia
14.
Int J Legal Med ; 133(1): 65-69, 2019 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-29460109

RESUMO

We examined an online sold product "Hatha Jodi" synonym of "paired arm" for the confirmation of its biological source. It was declared as a plant root. The morphological features of these samples were matched with the "intromittent organs" or "hemi penis" of the monitor lizard. For further confirmation, we used sequencing of a partial fragment of mitochondrial DNA cytochrome b gene. Sequence comparison indicated that these claimed plant products were actually biological samples of a common monitor lizard, Varanus bengalensis. Hence, it exhibited the ongoing illegal trade of the intromittent organ of a prohibited species with a misleading name using low risk and widely adopted modern trading method that imposes a severe challenge for combating against the wildlife crime.


Assuntos
Conservação dos Recursos Naturais , Crime , Citocromos b/genética , DNA Mitocondrial , Lagartos/genética , Animais , Comércio , Índia , Análise de Sequência de DNA
15.
Mol Biol Rep ; 46(1): 1327-1333, 2019 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-30456740

RESUMO

We report complete mitochondrial genome of Northern Indian red muntjac, Muntiacus vaginalis, and its phylogenetic inferences. Mitogenome composition was 16,352 bp in length and its overall base composition in the circular genome was A = 33.2%, T = 29.0%, C = 24.50% and G = 13.30%. It exhibited a typical mitogenome structure, including 22 transfer RNA genes, 13 protein-coding genes, two ribosomal RNA genes and a major non-coding control region (D-loop region). All the genes except ND6 and eight tRNA's were encoded on the heavy strand. Phylogenetic analyses showed that M. vaginalis is closely related to M. muntjak and formed a sister relationship with Elaphodus cephalophus. In view of the unclear distribution range and escalating habitat loss, it is important to identify its population genetic status. The complete mitogenome described in this study can be used in further phylogenetics, identification of extant maternal lineage, evolutionary significance unit and its genetic conservation.


Assuntos
Genoma Mitocondrial , Cervo Muntjac/genética , Filogenia , Animais , Teorema de Bayes , Índia , Cadeias de Markov , Método de Monte Carlo , Nucleotídeos/genética , Fases de Leitura Aberta/genética , RNA Ribossômico/genética , RNA de Transferência/genética
17.
Int J Mol Sci ; 20(1)2018 Dec 24.
Artigo em Inglês | MEDLINE | ID: mdl-30586858

RESUMO

Catechol dioxygenases in microorganisms cleave catechol into cis-cis-muconic acid or 2-hydroxymuconic semialdehyde via the ortho- or meta-pathways, respectively. The aim of this study was to purify, characterize, and predict the template-based three-dimensional structure of catechol 1,2-dioxygenase (C12O) from indigenous Pseudomonas chlororaphis strain UFB2 (PcUFB2). Preliminary studies showed that PcUFB2 could degrade 40 ppm of 2,4-dichlorophenol (2,4-DCP). The crude cell extract showed 10.34 U/mL of C12O activity with a specific activity of 2.23 U/mg of protein. A 35 kDa protein was purified to 1.5-fold with total yield of 13.02% by applying anion exchange and gel filtration chromatography. The enzyme was optimally active at pH 7.5 and a temperature of 30 °C. The Lineweaver⁻Burk plot showed the vmax and Km values of 16.67 µM/min and 35.76 µM, respectively. ES-MS spectra of tryptic digested SDS-PAGE band and bioinformatics studies revealed that C12O shared 81% homology with homogentisate 1,2-dioxygenase reported in other Pseudomonas chlororaphis strains. The characterization and optimization of C12O activity can assist in understanding the 2,4-DCP metabolic pathway in PcUFB2 and its possible application in bioremediation strategies.


Assuntos
Proteínas de Bactérias/metabolismo , Catecol 1,2-Dioxigenase/metabolismo , Pseudomonas chlororaphis/enzimologia , Sequência de Aminoácidos , Proteínas de Bactérias/química , Proteínas de Bactérias/classificação , Catecol 1,2-Dioxigenase/química , Catecol 1,2-Dioxigenase/classificação , Catecóis/metabolismo , Clorofenóis/química , Clorofenóis/metabolismo , Cromatografia em Gel , Cromatografia por Troca Iônica , Homogentisato 1,2-Dioxigenase/química , Homogentisato 1,2-Dioxigenase/metabolismo , Concentração de Íons de Hidrogênio , Cinética , Metais/química , Metais/metabolismo , Filogenia , Estabilidade Proteica , Estrutura Quaternária de Proteína , Alinhamento de Sequência , Especificidade por Substrato , Temperatura Ambiente
18.
Indian J Dermatol ; 63(6): 469-474, 2018.
Artigo em Inglês | MEDLINE | ID: mdl-30504974

RESUMO

Context: Illnesses affecting sexual organs and its correlation with improper sexual behavior lead to a negative attitude and discriminating behavior towards people affected by such disorders. Aim: The aim was to study the stigma associated with sexually transmitted infections (STIs) among patients attending Suraksha clinic at a tertiary care hospital in northern India. Settings and Design: This was a hospital-based cross-sectional study. Subjects and Methods: The present study was conducted at Suraksha (STI) clinic, King George's Medical University, Uttar Pradesh. A total of 487 STI patients (clinically and/or laboratory-confirmed) were contacted telephonically, of which 49 finally participated in the study. Stigma was assessed using modified and pretested version of India HIV-related stigma scale adapted in context to sexually transmitted diseases. Statistical Analysis: Quantitative variables were expressed as mean with standard deviation, and independent sample t-test was used to compare the mean values. P < 0.05 was considered statistically significant. Results: Mean scores of enacted, vicarious, felt normative, and internalized stigma for 49 patients (out 487) who finally participated in the study were 0.04±0.11, 0.55±0.70, 1.21±0.96, and 0.86±0.67, respectively. Unmarried/divorced/separated patients had significantly higher vicarious and felt normative stigma scores as compared to married individuals. Mean score for felt normative stigma was significantly higher among homosexual/bisexuals in comparison to heterosexual individuals. Conclusions: Efforts should be directed towards the provision of integrated services through sexual health-oriented campaigns to address the stigma associated with STI in a more comprehensive way.

19.
Indian J Otolaryngol Head Neck Surg ; 70(4): 544-548, 2018 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-30464913

RESUMO

Retrospective study of the prelingual cochlear implantation programme under government scheme done at medical college hospital in central India. Forty-two prelingually deaf children screened and sent for cochlear implantation at our centre From March 2015 to Feb 2018 were reviewed with respect to their age, sex, preimplantation hearing aid use, surgical technique for cochlear implantation, type of FDA (USA) approved cochlear implant, post operative speech therapy and its outcome with respect to categories of auditory perception and speech intelligibility scoring were compared for children younger than 4 years and older than 4 years. For outcome measurement non parametric statistical method was used for any significance between the two groups. There was a wide range of children implanted ranging from 2 to 7 years. Both varia and mastoidectomy and posterior tympanotomy method of cochlear implantations were done with good rate complete insertion and electrode activation. There was no significant difference between the two group with regard to CAP and SIR outcomes after 1 year. In order to get better outcomes with respect to the speech language development, there is need to strengthen the early identification and cochlear implantation before 4 years of age in government approved schemes.

20.
Sci Rep ; 8(1): 16308, 2018 11 05.
Artigo em Inglês | MEDLINE | ID: mdl-30397218

RESUMO

The hog deer (Axis porcinus) is threatened by habitat alteration, fragmentation, and poaching, which have led to a drastic decline of its wild population. Two subspecies of A. porcinus have been described from its distribution range. A. p. porcinus is reported to occur from Pakistan along the Himalayan foothills through Nepal, India and Myanmar, and A. p. annamiticus is found in Thailand, Indo-China, Laos, Cambodia, and Vietnam. However, the current distribution range of A. p. annamiticus is still unclear. We used the partial control region (CR) of mitochondrial DNA (mtDNA) and seven microsatellite loci to investigate the intra-species structure, differentiation, and demographic history of hog deer populations from three landscapes, the Terai Arc, Northeast, and Indo-Burma (Keibul Lamjao National Park (KLNP), Manipur, India) landscapes. We also carried out divergence time estimation using the complete mitogenome. The level of variation was ~4%, and the time of divergence of the KLNP population and the other Indian populations was about 0.22 Mya, i.e., during the last glaciation periods of the Late Pleistocene/Early Holocene. The KLNP haplotypes of the control region were shared with the Southeast Asian subspecies, A. p. annamiticus. The results of the investigations of the microsatellite loci supported the mtDNA results unambiguously. Two genetically distinct lineages are found in India: one is found from the Terai Arc to Assam (A. p. porcinus) and the other in Manipur (A. p. annamiticus). The genetic diversity in KLNP was low and exhibited a higher degree of genetic differentiation compared with major Indian populations. The Bayesian skyline plots indicated that after a long phase of historic demographic stability, the populations of both the lineages of hog deer suffered pronounced declines during the period from ~800 years BP to 5000 years BP. In summary, our finding provided evidence that the KLNP population is probably a prime, isolated and sustaining stock of A. p. annamiticus and should be managed as evolutionarily significant units (ESUs).


Assuntos
Cervos/genética , Animais , DNA Mitocondrial/genética , Ecossistema , Geografia , Haplótipos/genética , Índia , Filogenia , Polimorfismo Genético
SELEÇÃO DE REFERÊNCIAS
DETALHE DA PESQUISA