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1.
Opt Express ; 27(22): 31599-31620, 2019 Oct 28.
Artigo em Inglês | MEDLINE | ID: mdl-31684391

RESUMO

In this paper, we propose double junction tandem organic solar cells with PTB7:PC70BM and PDPPSDTPS:PC60BM as the polymeric active materials to cover the wide solar spectrum from 300 nm to 1150 nm. We present novel designs and finite-difference time-domain (FDTD) simulation results of plasmonic double junction tandem OSCs in which Ag nanospheres are present over the top surface of the OSC and Ag nanostars are present in the bottom subcell which substantially enhance the absorption, short circuit current density, and efficiency of the OSC as compared to the reference tandem OSCs that do not contain any nanoparticles. Different geometries of the plasmonic nanoparticles such as nanospheres and nanostars were used in the top subcell and the bottom subcell, respectively, so that the absorption in the different spectral regimes - corresponding to the bandgaps of the active layers in the two subcells (PTB7:PC70BM in the top subcell and LBG:PC60BM in the bottom subcell) - could be enhanced. The thickness of the bottom subcell active layer as well as the geometries of the plasmonic nanoparticles were optimized such that the short circuit current densities in the two subcells could be matched in the tandem OSC. An overall enhancement of 26% in the short circuit current density was achieved in a tandem OSC containing the optimized Ag nanospheres over the top surface and Ag nanostars inside the bottom subcell active layer. The presence of plasmonic nanoparticles along with the wide spectrum absorption band of the active materials in the tandem OSC leads to a typical power conversion efficiency of ∼ 15.4%, which is higher than that of a reference tandem organic solar cell (12.25%) that does not contain any nanoparticles.

2.
Rev Bras Anestesiol ; 69(5): 517-520, 2019.
Artigo em Português | MEDLINE | ID: mdl-31635757

RESUMO

OBJECTIVE AND BACKGROUND: Erector spinae plane block is a novel analgesic truncal block that has been popularized due to its ease of performance and perceived safety. Erector spinae plane block has been postulated to target the ventral rami and rami communicates of spinal nerves, thus providing somatic and visceral analgesia. In this case series, we describe our experience of bilateral erector spinae plane block placed at the low thoracic level in open gynecologic oncology surgery in three patients. METHOD: Under ultrasound guidance, erector spinae plane blocks were done, preoperatively, at the 8th thoracic transverse process bilaterally. Numeric rating scale for pain and opioid consumption of the first 48 postoperative hours were recorded. RESULTS: Pain scores ranged from 0 to 4 among the three patients and 48h opioid consumption in oral morphine equivalents of 4, 6 and 18mg. No adverse events were recorded up to patient discharge from the hospital. CONCLUSIONS: Erector spinae plane block provided effective analgesia in our case series. While its true mechanism of action remains obscure, the available case reports show encouraging analgesic results with no adverse events recorded. Formal prospective randomized trials are underway to provide further evidence on its efficacy, failure rate and safety.

3.
Reg Anesth Pain Med ; 2019 Oct 23.
Artigo em Inglês | MEDLINE | ID: mdl-31649029

RESUMO

BACKGROUND AND OBJECTIVES: The serratus plane block (SPB) is a novel chest wall interfascial plane block. Its analgesic efficacy compared with non-block care and paravertebral block (PVB) is unestablished. METHODS: We conducted a random-effects meta-analysis of randomized controlled trials (RCTs) recruiting adult surgical patients that compared a SPB to non-block care or PVB for postoperative analgesia. Visual analog scale pain scores were the primary outcome. Database sources were Medline, Embase, the Cochrane Library, and Google Scholar searched up to July 29, 2019 without language restriction. Risk of bias was assessed using Cochrane methodology. RESULTS: Nineteen RCTs that comprised 1260 patients were included. Six trials involved thoracic surgery patients and 13 studied breast surgery patients. SPB reduced pain scores 0 hour postoperatively (-1.62 cm; 99% CI -2.43 to -0.81; p<0.001; I2=92%), at 2-4 hours (-1.29 cm; 99% CI -2.08 to -0.49; p<0.001; I2=92%), at 6 hours (-1.69 cm; 99% CI -3.19 to -0.20; p=0.004; I2=99%), and up to 24 hours compared with non-block care. SPB also prolonged the time to first analgesic request (193.2 min; 95% CI 7.2 to 379.2 min; p=0.04; I2=99%), reduced 24-hour postoperative opioid consumption (-11.27 mg of IV morphine equivalent; -17.36 to -5.18 mg; p<0.001), and reduced postoperative nausea and vomiting (RR 0.51; 95% CI 0.38 to 0.68; p<0.001; I2=12%). In contrast, no meaningful differences were detected in any of the outcomes for the SPB versus PVB data. CONCLUSIONS: SPB reduced postoperative pain scores (Grading of Recommendations Assessment, Development, and Evaluation rating: low; due to heterogeneity and deficiencies in blinding) in breast and thoracic surgery patients compared with non-block care. Based on five trials only, SPB was not appreciably different from PVB.

4.
Chemistry ; 25(68): 15498-15503, 2019 Dec 05.
Artigo em Inglês | MEDLINE | ID: mdl-31518018

RESUMO

A "branching-folding" synthetic strategy that affords a range of diverse cyclic benzo-sulfonamide scaffolds is presented. Whereas different annulation reactions on common ketimine substrates build the branching phase of the scaffold synthesis, a common hydrogenative ring-expansion method, facilitated by an increase of the ring-strain during the branching phase, led to sulfonamides bearing medium-sized rings in a folding pathway. Cell painting assay was successfully employed to identify tubulin targeting sulfonamides as novel mitotic inhibitors.


Assuntos
Sulfonamidas/síntese química , Ciclização , Sulfonamidas/química
5.
J Anaesthesiol Clin Pharmacol ; 35(1): 19-24, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31057234

RESUMO

The ex utero intrapartum treatment (EXIT) procedure is performed in cases of fetal congenital malformation. The anesthetic management is much more challenging and involves providing profound uterine relaxation, maintenance of Uteroplacental blood flow and fetal anesthesia. The aim of the article is to review the literature and compare the efficacy of both the anesthetic techniques with respect to maternal and fetal outcomes. The literature source for this review was obtained via PubMed, Medline, Google scholar and Cochrane database of systematic reviews until January 2017. In our literature review we found that both GA and Regional anesthesia were successfully described for EXIT procedure but GA was performed in the majority of cases. Consideration for anesthetic technique should be done on a case-by-case basis.

6.
Chemistry ; 25(11): 2717-2722, 2019 Feb 21.
Artigo em Inglês | MEDLINE | ID: mdl-30474890

RESUMO

The first synthetic access to stable and acyclic 1,3-butadienes with two electron-withdrawing carbonyl groups and their potential to deliver new molecular scaffolds through intriguing endo-selective Diels-Alder cycloadditions are presented. The bicyclic scaffolds produced through the cycloaddition chemistry of electron-deficient dienes afforded potent Hedgehog signaling pathway inhibitors.

7.
Org Biomol Chem ; 17(3): 413-431, 2019 01 16.
Artigo em Inglês | MEDLINE | ID: mdl-30516778

RESUMO

Indole polycycles are common structural frameworks of biologically intriguing small molecules of natural and synthetic origin and therefore remain interesting and challenging synthetic targets. Cascade reactions wherein a number of reactions occur in a sequential manner in the same reaction apparatus are highly efficient chemical processes which quickly build up molecular complexity. Synthetic approaches based on cascade reactions are highly useful as they tend to avoid multiple reaction work-up steps as well as purifications of all intermediary products. Therefore, in the last decade, a number of cascade reaction based approaches to build various molecular scaffolds of biological interest have been reported. However, a relatively smaller number of cascade reaction based synthetic strategies have targeted the indole polycycles. In this article, we have summarized some interesting cascade reaction based synthesis designs leading to complex indole polycycles including some biologically intriguing and natural product inspired indole frameworks.


Assuntos
Produtos Biológicos/síntese química , Indóis/síntese química , Compostos Policíclicos/síntese química , Produtos Biológicos/química , Indóis/química , Estrutura Molecular , Compostos Policíclicos/química
8.
J Sleep Res ; 28(2): e12725, 2019 04.
Artigo em Inglês | MEDLINE | ID: mdl-30033688

RESUMO

Knowing how an individual responds to sleep deprivation is a requirement for developing personalized fatigue management strategies. Here we describe and validate the 2B-Alert App, the first mobile application that progressively learns an individual's trait-like response to sleep deprivation in real time, to generate increasingly more accurate individualized predictions of alertness. We incorporated a Bayesian learning algorithm within the validated Unified Model of Performance to automatically and gradually adapt the model parameters to an individual after each psychomotor vigilance test. We implemented the resulting model and the psychomotor vigilance test as a smartphone application (2B-Alert App), and prospectively validated its performance in a 62-hr total sleep deprivation study in which 21 participants used the app to perform psychomotor vigilance tests every 3 hr and obtain real-time individualized predictions after each test. The temporal profiles of reaction times on the app-conducted psychomotor vigilance tests were well correlated with and as sensitive as those obtained with a previously characterized psychomotor vigilance test device. The app progressively learned each individual's trait-like response to sleep deprivation throughout the study, yielding increasingly more accurate predictions of alertness for the last 24 hr of total sleep deprivation as the number of psychomotor vigilance tests increased. After only 12 psychomotor vigilance tests, the accuracy of the model predictions was comparable to the peak accuracy obtained using all psychomotor vigilance tests. With the ability to make real-time individualized predictions of the effects of sleep deprivation on future alertness, the 2B-Alert App can be used to tailor personalized fatigue management strategies, facilitating self-management of alertness and safety in operational and non-operational settings.

9.
J Cell Biol ; 217(12): 4199-4214, 2018 12 03.
Artigo em Inglês | MEDLINE | ID: mdl-30309979

RESUMO

SipA is a major effector of Salmonella, which causes gastroenteritis and enteric fever. Caspase-3 cleaves SipA into two domains: the C-terminal domain regulates actin polymerization, whereas the function of the N terminus is unknown. We show that the cleaved SipA N terminus binds and recruits host Syntaxin8 (Syn8) to Salmonella-containing vacuoles (SCVs). The SipA N terminus contains a SNARE motif with a conserved arginine residue like mammalian R-SNAREs. SipAR204Q and SipA1-435R204Q do not bind Syn8, demonstrating that SipA mimics a cognate R-SNARE for Syn8. Consequently, Salmonella lacking SipA or that express the SipA1-435R204Q SNARE mutant are unable to recruit Syn8 to SCVs. Finally, we show that SipA mimicking an R-SNARE recruits Syn8, Syn13, and Syn7 to the SCV and promotes its fusion with early endosomes to potentially arrest its maturation. Our results reveal that SipA functionally substitutes endogenous SNAREs in order to hijack the host trafficking pathway and promote Salmonella survival.


Assuntos
Proteínas de Bactérias/metabolismo , Endossomos/metabolismo , Interações Hospedeiro-Patógeno , Fusão de Membrana , Proteínas dos Microfilamentos/metabolismo , Proteínas Qa-SNARE/metabolismo , Salmonella/fisiologia , Proteínas de Bactérias/genética , Endossomos/microbiologia , Células HeLa , Humanos , Proteínas dos Microfilamentos/genética , Proteínas Qa-SNARE/genética
10.
Cell Chem Biol ; 25(9): 1095-1106.e23, 2018 09 20.
Artigo em Inglês | MEDLINE | ID: mdl-30251630

RESUMO

Identification and validation of the targets of bioactive small molecules identified in cell-based screening is challenging and often meets with failure, calling for the development of new methodology. We demonstrate that a combination of chemical proteomics with in silico target prediction employing the SPiDER method may provide efficient guidance for target candidate selection and prioritization for experimental in-depth evaluation. We identify 5-lipoxygenase (5-LO) as the target of the Wnt pathway inhibitor Lipoxygenin. Lipoxygenin is a non-redox 5-LO inhibitor, modulates the ß-catenin-5-LO complex and induces reduction of both ß-catenin and 5-LO levels in the nucleus. Lipoxygenin and the structurally unrelated 5-LO inhibitor CJ-13,610 promote cardiac differentiation of human induced pluripotent stem cells and inhibit Hedgehog, TGF-ß, BMP, and Activin A signaling, suggesting an unexpected and yet unknown role of 5-LO in these developmental pathways.


Assuntos
Araquidonato 5-Lipoxigenase/metabolismo , Inibidores de Lipoxigenase/química , Inibidores de Lipoxigenase/farmacologia , Proteômica/métodos , Transdução de Sinais/efeitos dos fármacos , Animais , Diferenciação Celular/efeitos dos fármacos , Linhagem Celular , Linhagem Celular Tumoral , Simulação por Computador , Projeto Auxiliado por Computador , Células HEK293 , Proteínas Hedgehog/metabolismo , Humanos , Células-Tronco Pluripotentes Induzidas/citologia , Células-Tronco Pluripotentes Induzidas/efeitos dos fármacos , Células-Tronco Pluripotentes Induzidas/metabolismo , Camundongos , Células NIH 3T3 , Via de Sinalização Wnt/efeitos dos fármacos
11.
J Complement Integr Med ; 16(1)2018 Jun 21.
Artigo em Inglês | MEDLINE | ID: mdl-29933244

RESUMO

Background & methods We investigated the in vitro antioxidant and antifungal activity by agar disc diffusion assay of leaf extract of some stinging plants namely, Urtica dioica L., Tragia involucrate L., Carduus nutans L. and Mucuna pruriens (L.) DC., against pathogenic fungi causing infections/diseases. Results M. pruriens (Disc 4), T. involucrate (Disc 2), U. dioica (Disc 1) showed significant antifungal activity against all tested pathogens, while C. nutans (Disc 3) showed intermediate activity against only Chaetomium globosum (Cg). The leaf extract of M. Pruriens showed maximum total phenol content (~1004 µg g-1 dry wt) followed by T. involucrate, C. nutans and U. dioica. However, the ascorbate was observed highest in T. involucrate (~10.3 µg g-1 dry wt) followed by M. pruriens (~9.2 µg g-1 dry wt) but the difference was not significant (p ≤ 0.05). Likewise, M. pruriens showed maximum anthocyanin content (~0.3 µg g-1 dry wt). The activity of antioxidant enzymes revealed that M. Pruriens showed maximum ascorbate peroxidase (APX) activity, while the highest guaiacol peroxidase (GPX) and catalase (CAT) activities were observed in C. nutans and U. dioica, respectively. Conclusions M. Pruriens showed potential in vitro antioxidant and antifungal activity against studied pathogens that may be used for ethno-pharmacological uses.


Assuntos
Antifúngicos/farmacologia , Antioxidantes/farmacologia , Carduus/química , Fungos/efeitos dos fármacos , Mucuna/química , Extratos Vegetais/farmacologia , Técnicas In Vitro , Micoses/tratamento farmacológico , Extratos Vegetais/química , Urtica dioica/química
12.
ChemistryOpen ; 7(4): 302-309, 2018 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-29721402

RESUMO

Complexity-generating chemical transformations that afford novel molecular scaffolds enriched in sp3 character are highly desired. Here, we present a highly stereoselective scaffold diversity synthesis approach that utilizes cascade double-annulation reactions of diverse pairs of zwitterionic and non-zwitterionic partners with 3-formylchromones to generate highly complex tetracyclic benzopyrones. Each pair of annulation partners adds to the common chroman-4-one scaffold to build two new rings, supporting up to four contiguous chiral centers that include an all-carbon quaternary center. Differently ring-fused benzopyrones display different biological activities, thus demonstrating their immense potential in medicinal chemistry and chemical biology research.

13.
J Neurosci Methods ; 304: 39-45, 2018 07 01.
Artigo em Inglês | MEDLINE | ID: mdl-29679703

RESUMO

BACKGROUND: The psychomotor vigilance task (PVT) has been widely used to assess the effects of sleep deprivation on human neurobehavioral performance. To facilitate research in this field, we previously developed the PC-PVT, a freely available software system analogous to the "gold-standard" PVT-192 that, in addition to allowing for simple visual reaction time (RT) tests, also allows for near real-time PVT analysis, prediction, and visualization in a personal computer (PC). NEW METHOD: Here we present the PC-PVT 2.0 for Windows 10 operating system, which has the capability to couple PVT tests of a study protocol with the study's sleep/wake and caffeine schedules, and make real-time individualized predictions of PVT performance for such schedules. We characterized the accuracy and precision of the software in measuring RT, using 44 distinct combinations of PC hardware system configurations. RESULTS: We found that 15 system configurations measured RTs with an average delay of less than 10 ms, an error comparable to that of the PVT-192. To achieve such small delays, the system configuration should always use a gaming mouse as the means to respond to visual stimuli. We recommend using a discrete graphical processing unit for desktop PCs and an external monitor for laptop PCs. COMPARISON WITH EXISTING METHOD: This update integrates a study's sleep/wake and caffeine schedules with the testing software, facilitating testing and outcome visualization, and provides near-real-time individualized PVT predictions for any sleep-loss condition considering caffeine effects. CONCLUSIONS: The software, with its enhanced PVT analysis, visualization, and prediction capabilities, can be freely downloaded from https://pcpvt.bhsai.org.


Assuntos
Microcomputadores , Desempenho Psicomotor/fisiologia , Sono/fisiologia , Software , Vigília/fisiologia , Adulto , Atenção , Cafeína/farmacologia , Humanos , Masculino , Valor Preditivo dos Testes , Tempo de Reação/fisiologia , Vigília/efeitos dos fármacos
14.
Plant Cell Environ ; 41(9): 2128-2140, 2018 09.
Artigo em Inglês | MEDLINE | ID: mdl-29492990

RESUMO

Ascochyta blight (AB) caused by the fungal pathogen Ascochyta rabiei is a serious foliar disease of chickpea (Cicer arietinum L.). Despite many genetic studies on chickpea-Ascochyta interaction, genome-wide scan of chickpea for the identification of AB-associated quantitative trait loci (QTLs) and their gene(s) has not been accomplished. To elucidate narrow QTLs for AB resistance, here, we report the use of multiple QTL-sequencing approach on 2 sets of extreme AB phenotype bulks derived from Cicer intraspecific and interspecific crosses. Two major QTLs, qABR4.1 and qABR4.2, and a minor QTL, qABR4.3, were identified on assembled chickpea pseudomolecule 4. We narrowed qABR4.1 to a "robust region" at 4.568-4.618 Mb through mapping on a larger intraspecific cross-derived population and comparative analysis. Among 4 genes, the CaAHL18 gene showed higher expression under Ascochyta stress in AB resistant parent suggesting that it is the candidate gene under "robust qABR4.1." Dual-luciferase assay with CaAHL18 polymorphic cis-regulatory sequences showed that allelic variation is associated with higher expression. Thus, our findings on chickpea-Ascochyta interaction have narrowed down AB resistance associated QTLs on chickpea physical map. The narrowed QTLs and gene-associated markers will help in biotechnological and breeding programs for chickpea improvement.


Assuntos
Ascomicetos/patogenicidade , Cicer/genética , Cicer/microbiologia , Proteínas de Plantas/genética , Locos de Características Quantitativas , Motivos AT-Hook , Mapeamento Cromossômico , Cruzamentos Genéticos , Resistência à Doença/genética , Regulação da Expressão Gênica de Plantas , Marcadores Genéticos , Genoma de Planta , Família Multigênica , Doenças das Plantas/microbiologia , Proteínas de Plantas/metabolismo , Plantas Geneticamente Modificadas , Reprodutibilidade dos Testes , Tabaco/genética , Técnicas do Sistema de Duplo-Híbrido
15.
Indian J Endocrinol Metab ; 22(1): 47-49, 2018.
Artigo em Inglês | MEDLINE | ID: mdl-29535936

RESUMO

Introduction: Diabetes is a major world-wide healthcare problem. Cost effective markers for screening and diagnosis of T2DM are the need of the day especially in developing and under-developed countries. Simple anthropometric measurements may help us in identifying individuals likely to have diabetes. Material and Methods: Data from 1055 North-Indian subjects was analysed. Results: Out of several anthropometric measurements studied, Waist-Thigh ratio (WTR) correlated significantly and positively with all three measures of diabetes i.e. FPG, RPG and PPG. (P < .0001) suggesting that it is the best predictor of diabetes. Subjects with diabetes had greater WTR (mean 2.088) than those without (mean1.842). (P < .0001). A thresh-hold effect was evident at a cut-off WTR of 2.3. Out of those subjects who were diagnosed to have diabetes by AACE/AHA guidelines, 82% had WTR greater than this value (P < 0.001). Conclusion: WTR may prove to be a simple and inexpensive marker for detecting Type 2 diabetes. Larger studies are required to develop population norms.

16.
Angew Chem Int Ed Engl ; 57(19): 5212-5226, 2018 05 04.
Artigo em Inglês | MEDLINE | ID: mdl-29105306

RESUMO

Chemical tools that enable a catalytic reaction to selectively and efficiently yield different products will allow charting of wider chemical space. In ligand-directed divergent synthesis, a common mode of catalysis is modulated by employing different ligands for catalytic organometallic complexes to transform either common substrates or common reactive intermediates into distinct molecular scaffolds. The strategy has the potential to create important and diverse scaffolds and to unveil novel modes of catalytic transformations for wider synthetic applications. This strategy is described and recent efforts in this emerging field of catalysis, focusing on transition-metal catalysis for the synthesis of carbo- and heterocyclic ring systems, are reviewed.

17.
Appl Spectrosc ; 72(4): 618-626, 2018 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-29164904

RESUMO

This paper presents a set of system validation experiments that can be used to qualify either static or flow experimental systems for gathering tracer photophysical data or conducting laser diagnostics at high pressure and temperature in order to establish design and operation limits and reduce uncertainty in data interpretation. Tests demonstrated here quantify the effect of tracer absorption at the test cell walls, stratification, photolysis, pyrolysis, adequacy of mixing and seeding, and reabsorption of laser light using acetone as the tracer and 282 nm excitation. Results show that acetone exhibits a 10% decrease in fluorescence signal over 36 000 shots at 127.4 mJ/cm2, and photolysis is negligible below 1000 shots collected. Meanwhile, appropriately chosen gas residence times can mitigate risks due to pyrolysis and inadequate mixing and seeding; for the current work 100 ms residence time ensured <0.5% alteration of tracer number density due to thermal destruction. Experimental results here are compared to theoretical values from the literature.

18.
Angew Chem Int Ed Engl ; 56(50): 15945-15949, 2017 12 11.
Artigo em Inglês | MEDLINE | ID: mdl-29083515

RESUMO

The active complexes of chiral N,N'-dioxide ligands with dysprosium and magnesium salts catalyze the hetero-Diels-Alder reaction between 2-aza-3-silyloxy-butadienes and alkylidene oxindoles to selectively form 3,3'- and 3,4'-piperidinoyl spirooxindoles, respectively, in very high yields and with excellent enantioselectivities. The exo-selective asymmetric cycloaddition successfully regaled the construction of sp3 -rich and highly substituted natural-product-based spirooxindoles supporting many chiral centers, including contiguous all-carbon quaternary centers.

19.
Chimia (Aarau) ; 71(10): 653-660, 2017 Oct 25.
Artigo em Inglês | MEDLINE | ID: mdl-29070410

RESUMO

Fragment-based ligand discovery is a key technology to develop lead structures for drug discovery. The majority of the fragments employed so far is aromatic and sp2-configured, and there is a high demand of fragments with stereogenic centers. Natural products (NPs) are evolutionary selected ligands for a range of diverse macromolecular targets. Small-sized molecules - fragments - based on NPs may inherit the biological relevance of nature's treasure and could offer novel opportunities to engage challenging protein targets. An overview of this emerging research area is presented. The deconstruction of a complex NP into small fragments marks the beginning of this journey that is facilitated by the synthesis of NP-based 3D fragments. The emerging strategies in organic synthesis for either degradation of NPs to access fragments or de novo construction of fragments and their further combinations to chart novel biologically relevant chemical space is discussed.


Assuntos
Produtos Biológicos , Descoberta de Drogas , Ligantes , Bibliotecas de Moléculas Pequenas
20.
Blood Cells Mol Dis ; 66: 31-36, 2017 07.
Artigo em Inglês | MEDLINE | ID: mdl-28810169

RESUMO

Low plasma TFPI levels have been associated with an increased risk of DVT; however its association with TFPI gene polymorphisms is controversial and not yet studied in India. The aim of our study was to analyze prevalence of TFPI gene polymorphisms, evaluate their effects on its plasma levels and determine its association with DVT. Plasma level and genetic polymorphisms (33T>C, 399C>T and 536C>T) of TFPI were screened in subjects (100 DVT patients and 100 controls). Mean TFPI level in patients was significantly lower than controls (Patients: 33.55±11.72ng/ml, Controls: 48.05±13.68ng/ml, p<0.001). DVT patients had significantly higher prevalence of 399C>T (p=0.001, ORa: 5.69, CI: 1.14-28.46) and lower prevalence of 33T>C polymorphism (p<0.001, ORa: 0.239, CI: 0.065-0.871). The wild type (TT genotype) of 33T>C and variant form (CT and TT genotype) of 399C>T polymorphism was significantly associated with low TFPI levels. TFPI 536C>T polymorphism was absent in all subjects. In conclusion, dual nature of TFPI gene polymorphisms were established in our association study; 33T>C being protective and 399C>T as an important risk factor in Indian DVT patients, probably mediated by alteration in TFPI levels. These findings may prove a vital role in risk stratification and treatment of DVT.


Assuntos
Lipoproteínas/sangue , Polimorfismo de Nucleotídeo Único/genética , Trombose Venosa/genética , Adulto , Estudos de Casos e Controles , Humanos , Índia/epidemiologia , Epidemiologia Molecular , Prevalência , Fatores de Risco , Trombose Venosa/sangue , Trombose Venosa/epidemiologia
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