Your browser doesn't support javascript.
loading
Mostrar: 20 | 50 | 100
Resultados 1 - 20 de 1.037
Filtrar
1.
Head Neck Pathol ; 2021 Feb 28.
Artigo em Inglês | MEDLINE | ID: mdl-33646558

RESUMO

Prior studies have established the carcinogenic role of HPV16 and also demonstrated its unique biological behavior in cervical and oropharyngeal squamous cell carcinoma (OPSCC) but its role in oral leukoplakia (OL) and oral squamous cell carcinoma (OSCC) is not well explored. Therefore, in the present study, we assessed HPV16 prevalence using PCR and Anti-HPV16 antibodies for the first time and correlated its biological behavior using p16INK4a and Ki67 proliferation index (PI) in OL, OSCC, and OPSCC. This study included 63 subjects comprising of 25 OL, 26 OSCC, and 12 OPSCC cases. Exfoliated cells were collected and processed for PCR followed by immunohistochemistry with primary antibodies p16INK4a, Anti-HPV16, and Ki67. The expressions were evaluated and statistical analysis included Chi-square and Spearman's test. Cumulatively 37% (OL-7%, OSCC-14% & OPSCC-16%) of cases showed positive PCR expression. PCR positivity was observed to be significantly higher (p 0.00) in OPSCC (9/12) than OSCC (9/26) and OL (5/25) cases. Overall immunohistochemical expression of p16INK4a, Anti-HPV16, and Ki67 were significantly (p 0.02) higher in HPV16 (PCR) positive cases. HPV16 + OSCC cases showed higher grades of p16INK4a and Ki67 expression. We have demonstrated a prevalence of HPV16 in OL, OSCC, and OPSCC through PCR, which may be concluded as a gold standard for the detection of HPV16 DNA.

2.
FEMS Microbiol Lett ; 2021 Feb 23.
Artigo em Inglês | MEDLINE | ID: mdl-33620442

RESUMO

The stringent response involves accumulation of (p)ppGpp, and it ensures that survival is prioritized. Production of (p)ppGpp requires purine synthesis, and upregulation of an operon that encodes the purine salvage enzyme xanthine dehydrogenase (Xdh) has been observed during stringent response in some bacterial species, where direct binding of ppGpp to a TetR-family transcription factor is responsible for increased xdh gene expression. We show here that the plant pathogen Ralstonia solanacearum has a regulatory system in which the LysR-family transcription factor XanR controls expression of the xan operon; this operon encodes Xdh as well as other enzymes involved in purine salvage, which favor accumulation of xanthine. XanR bound upstream of the xan operon, a binding that was attenuated on addition of either ppGpp or cyclic di-guanosine monophosphate (c-di-GMP). Using a reporter in which enhanced green fluorescent protein (EGFP) is expressed under control of a modified xan promoter, XanR was shown to repress EGFP production. Our data suggest that R. solanacearum features a regulatory mechanism in which expression of genes encoding purine salvage enzymes is controlled by a transcription factor that belongs to a different protein family, yet performs similar regulatory functions.

3.
Neurochem Res ; 2021 Feb 08.
Artigo em Inglês | MEDLINE | ID: mdl-33559104

RESUMO

Cocaine is a highly addictive stimulant and a well-known drug, with multiple effects on physiology. Cocaine can have direct effects on all cell types in the brain, including microglia. Microglia can be activated by other conditions, such as infection, inflammation, or injury. However, how cocaine regulates microglia and the influence of cocaine on microglial-derived exosomes remains unknown. Exosomes are nanovesicles that are responsible for intercellular communications, signaling, and trafficking necessary cargo for cell homeostasis. In this study, we hypothesized that cocaine affects exosome biogenesis and composition in BV2 microglial cells. BV2 microglial cells were cultured in exosome-depleted RPMI-1640 media and were treated according to the experimental designs. We observed that cell viability decreased by 11% at 100 µM cocaine treatment but was unaffected at other concentrations. After treatments, the exosomes were isolated from the condition media. Purified exosomes were characterized and quantified using transmission electron microscope (TEM) and nanoparticle tracking analysis (NTA). By NTA, there was a significant decrease in particles/mL after cocaine treatment. There was a 39.5%, 58.1%, 32.3% and 28.1% decrease in particles/mL at 100 nM, 1 µM, 10 µM and 100 µM cocaine, respectively. The characterization of exosomes and exosomal protein was performed by western/dot blot analyses. Tetraspanins CD11b, CD18 and CD63 were relatively unchanged after cocaine treatment. The heat shock proteins (Hsps), Hsp70 and Hsp90, were both significantly increased at 10 µM and 100 µM, but only hsp70 was significantly increased at 10 nM. The Rab proteins were assessed to investigate their role in cocaine-mediated exosomal decrease. Rab11 was significantly decreased at 10 nM, 100 nM, 1 µM, 10 µM and 100 µM by 15%, 28%, 25%, 38% and 22%, respectively. Rab27 was decreased at all concentrations but only significantly decreased at 100 nM, 1 µM and 100 µM cocaine by 21%, 24% and 23%, respectively. Rab35 had no significant changes noted when compared to control. Rab7 increased at all cocaine concentrations but only a significant increase in expression at 100 nM and 10 µM by 1.32-fold and 1.4-fold increase. Cocaine was found to alter exosome biogenesis and composition in BV2 microglial cells. Western and dot blot analyses verified the identities of purified exosomes, and the specific protein compositions of exosomes were found to change in the presence of cocaine. Furthermore, cocaine exposure modulated the expression of exosomal proteins, such as Hsps and Rab GTPases, suggesting the protein composition and formation of microglial-derived exosomes were regulated by cocaine.

4.
Curr Microbiol ; 2021 Feb 09.
Artigo em Inglês | MEDLINE | ID: mdl-33559732

RESUMO

Extracellular vesicles (EVs) play a fundamental role in cell and infection biology and have the potential to act as biomarkers for novel diagnostic tools. In this study, we explored the in vitro impact of bacterial lipopolysaccharide administration on cell lines that represents a target for bacterial infection in the host. Administration of lipopolysaccharide at varying concentrations to A549 and BV-2 cell lines caused only modest changes in cell death, but EV numbers were significantly changed. After treatment with the highest concentration of lipopolysaccharide, EVs derived from A549 cells packaged significantly less interleukin-6 and lysosomal-associated membrane protein 1. EVs derived from BV-2 cells packaged significantly less tumor necrosis factor after administration of lipopolysaccharide concentrations of 0.1 µg/mL and 1 µg/mL. We also examined the impact of lipopolysaccharide administration on exosome biogenesis and cargo composition in BALB/c mice. Serum-isolated EVs from lipopolysaccharide-treated mice showed significantly increased lysosomal-associated membrane protein 1 and toll-like receptor 4 levels compared with EVs from control mice. In summary, this study demonstrated that EV numbers and cargo were altered using these in vitro and in vivo models of bacterial infection.

5.
J Food Prot ; 2021 Feb 09.
Artigo em Inglês | MEDLINE | ID: mdl-33561184

RESUMO

Ruminants are natural reservoirs of Shiga toxin producing Escherichia coli (STEC), and the STEC can be easily transferred to carcasses during the conversion of animals to meat. Three experiments were conducted to validate the efficacy of lactic acid (4%; LA), peroxyacetic acid (300 ppm; PAA), and hot water (80˚C; HW) for their individual or combined abilities to reduce STEC surrogates on bob veal carcasses pre- and post-chill and through fabrication. In experiment 1, hot carcasses (n=9) were inoculated with a 5-strain cocktail (ca. 8 log CFU/mL) containing rifampicin-resistant surrogate Escherichia coli ( E. coli ; BAA-1427, BAA-1428, BAA-1429, BAA-1430, and BAA-1431) and then treated with HW, LA, or PAA. Carcasses were then chilled (0±1°C; 24 h), split in half, and each side was treated with either LA or PAA. In experiment 2, hot carcasses (n=3) were inoculated and chilled (24 h). After 24 h, the carcasses were split, and each side was treated with either LA or PAA. For experiment 3, carcasses (n=3) were chilled for 24 h, split, inoculated, and treated with either LA or PAA. After chilling, carcasses from all three experiments were fabricated to subprimals and the cut surfaces were sampled to determine the translocation. Experiment 1 showed that LA+LA was the most effective ( P ≤ 0.05) treatment for reducing surrogate E. coli on veal. In experiments 2 and 3, LA and PAA were similar ( P > 0.05) in their abilities to reduce E. coli on chilled veal carcasses. In experiments 1 and 2, all antimicrobial treatments resulted in undetectable levels (< 0.2 log CFU/cm 2 ) of surrogate E. coli on cut surfaces after fabrication, while low levels (1.7 and 1.0 log CFU/cm 2 for LA and PAA, respectively) were observed in experiment 3. Of the antimicrobial interventions utilized, lactic acid was more effective for reducing STEC surrogate populations on veal carcasses, pre- and/or post-chill.

6.
Artigo em Inglês | MEDLINE | ID: mdl-33550469

RESUMO

Polycyclic aromatic hydrocarbons (PAHs), organochlorine pesticides (OCPs) and phenolic compounds (PCs) are persistent organic compounds. Contamination of these potentially toxic organic pollutants in soils and sediments is most studied environmental compartments. In recent past, studies were carried out on PAHs, OCPs and PCs in various soils and sediments in India. But, this is the first study on these pollutants in soils and sediments from an urbanized river flood plain area in Delhi, India. During 2018, a total of fifty-four samples including twenty-seven each of soil and sediment were collected and analyzed for thirteen priority PAHs, four OCPs and six PCs. The detected concentration of ∑PAHs, ∑OCPs and ∑PCs in soils ranged between 473 and 1132, 13 and 41, and 639 and 2112 µg/kg, respectively, while their concentrations in sediments ranged between 1685 and 4010, 4.2 and 47, and 553 and 20,983 µg/kg, respectively. PAHs with 4-aromatic rings were the dominant compounds, accounting for 51 and 76% of total PAHs in soils and sediments, respectively. The contribution of seven carcinogen PAHs (7CPAHs) in soils and sediments accounted for 43% and 61%, respectively, to ∑PAHs. Among OCPs, p, p'-DDT was the dominant compound in soils, while α-HCH was found to be dominated in sediments. The concentrations of ∑CPs (chlorophenols) were dominated over ∑NPs (nitrophenols) in both the matrices. Various diagnostic tools were applied for the identification of their possible sources in soil and sediments. The observed concentrations of PAHs, OCPs and PCs were more or less comparable with the recently reports from various locations around the world including India. Soil quality guidelines and consensus-based sediment quality guidelines were applied for the assessment of ecotoxicological health effect.

7.
Crit Rev Biotechnol ; : 1-25, 2021 Feb 14.
Artigo em Inglês | MEDLINE | ID: mdl-33586555

RESUMO

The current trends of consumer-driven demands for natural therapeutics and the availability of evidence-based phytopharmaceuticals from traditional knowledge has once again brought the medicinal plants into forefront of health. In 2019, World Health Organization global report on traditional and complementary medicine has also substantiated the revival of herbal medicine including its convergence with conventional medicine for the management and prevention of diseases. It means these industries need plenty of plant materials to meet the unprecedented demands of herbal formulations. However, it is pertinent to mention here that around 70-80% medicinal plants are sourced from the wild and most of such highly acclaimed plants are listed under Rare, Endangered and Threatened species by IUCN. Additionally, over 30% traditional health formulations are based on underground plant parts, which lead to the uprooting of plants. Overharvesting from limited plant populations, meager conventional cultivation and a rising fondness for natural products exerting enormous pressure on natural habitats. Therefore, the nondestructive means of phytochemical production employing biotechnological tools could be used for sustainable production and consumption patterns. In recent years, a number of reports described the use of adventitious roots induced under in vitro conditions for the extraction of phytochemicals on a sustainable basis. In this article, efforts are made to review recent developments in this area as well as understand the induction mechanisms of adventitious roots, their in vitro cultivation, probable factors that affect the growth and metabolite production, and assess the possibility of industrial scale production to meet the rising demands of natural herbs.

8.
Sci Rep ; 11(1): 3702, 2021 Feb 12.
Artigo em Inglês | MEDLINE | ID: mdl-33580100

RESUMO

Rheum australe is an endangered medicinal herb of high altitude alpine region of Himalayas and is known to possess anti-cancerous properties. Unlike many herbs of the region, R. australe has broad leaves. The species thrives well under the environmental extremes in its niche habitat, therefore an understanding of transcriptome of R. australe to environmental cues was of significance. Since, temperature is one of the major environmental variables in the niche of R. australe, transcriptome was studied in the species growing in natural habitat and those grown in growth chambers maintained at 4 °C and 25 °C to understand genes associated with different temperatures. A total of 39,136 primarily assembled transcripts were obtained from 10,17,74,336 clean read, and 21,303 unigenes could match to public databases. An analysis of transcriptome by fragments per kilobase of transcript per million, followed by validation through qRT-PCR showed 22.4% up- and 22.5% down-regulated common differentially expressed genes in the species growing under natural habitat and at 4 °C as compared to those at 25 °C. These genes largely belonged to signaling pathway, transporters, secondary metabolites, phytohormones, and those associated with cellular protection, suggesting their importance in imparting adaptive advantage to R. australe in its niche.

9.
Food Chem ; 347: 128932, 2021 Jun 15.
Artigo em Inglês | MEDLINE | ID: mdl-33465692

RESUMO

The human immunodeficiency virus interacts with the cluster of differentiation 4 receptors and one of the two chemokine receptors (CCR5 and CXCR4) to gain entry in human cells. Both the co-receptors are essential for viral entry, replication, and are considered critical targets for antiviral drugs. In this study, bioactive molecules from different Himalayan plants were screened considering their potential to bind with the CCR5 and CXCR4 co-receptors. We utilized computational and thermodynamic parameters to validate the binding of the selected biomolecules to the active site of the co-receptors. The molecules Butyl 2-ethylhexyl phthalate and Dactylorhin-A showed a higher binding affinity with CCR5 co-receptor than the standard antagonist Maraviroc. Moreover, Pseudohypericin, Amarogentin, and Dactylorhin-E exhibited stronger interactions with CXCR4 than the co-crystallized inhibitor Isothiourea-1 t. Hence, we suggest that these molecules could be developed as potential inhibitors of the CCR5 and CXCR4 co-receptors. However, this require further in-vitro and in-vivo validation.

10.
Food Chem ; 346: 128933, 2021 Jun 01.
Artigo em Inglês | MEDLINE | ID: mdl-33418408

RESUMO

Immensely aggravated situation of COVID-19 has pushed the scientific community towards developing novel therapeutics to fight the pandemic. Small molecules can possibly prevent the spreading infection by targeting specific vital components of the viral genome. Non-structural protein 15 (Nsp15) has emerged as a promising target for such inhibitor molecules. In this investigation, we docked bioactive molecules of tea onto the active site of Nsp15. Based on their docking scores, top three molecules (Barrigenol, Kaempferol, and Myricetin) were selected and their conformational behavior was analyzed via molecular dynamics simulations and MMPBSA calculations. The results indicated that the protein had well adapted the ligands in the binding pocket thereby forming stable complexes. These molecules displayed low binding energy during MMPBSA calculations, substantiating their strong association with Nsp15. The inhibitory potential of these molecules could further be examined by in-vivo and in-vitro investigations to validate their use as inhibitors against Nsp15 of SARS-CoV2.


Assuntos
Antivirais/farmacologia , Simulação por Computador , Endorribonucleases/antagonistas & inibidores , Extratos Vegetais/farmacologia , Chá/química , Proteínas não Estruturais Virais/antagonistas & inibidores , Domínio Catalítico , Endorribonucleases/química , Endorribonucleases/metabolismo , Humanos , Ligantes , Simulação de Dinâmica Molecular , Proteínas não Estruturais Virais/química , Proteínas não Estruturais Virais/metabolismo
12.
Nurse Educ Today ; 96: 104640, 2021 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-33152641

RESUMO

BACKGROUND: The high focus states of India have higher maternal and neonatal mortality rates as compared to the national average. The quality of pre-service education (PSE) in nursing midwifery institutions in these states was found to be suboptimal. In 2013, Government of India implemented the PSE strengthening program across all public sector nursing midwifery institutions. The program focused on strengthening educational processes, training infrastructure, institutional management and clinical site practices by implementing a set of performance standards. OBJECTIVE: To evaluate the effect of PSE strengthening program on institutional performance and maternal neonatal health (MNH) related competences of nursing midwifery students and faculty. DESIGN: Single group pre-post intervention study. SETTINGS: 15 sampled public sector nursing midwifery institutions in the states of Madhya Pradesh and Odisha, India. PARTICIPANTS: Final-year students and faculty involved in teaching MNH subjects. METHODS: Performance of 15 sampled institutions was assessed at baseline, midline and endline using the performance standards. Additionally, competences of 232, 295 and 298 students and 64, 62 and 63 faculty members at baseline, midline and endline respectively were assessed on six MNH related practices using objective structured clinical examination (OSCE). RESULTS: None of the institutions met 70% standards during baseline. At endline, 13 of the 15 institutions met these standards. The mean OSCE scores of students and faculty at baseline was 17.1 (SD: 8.0) and 23.5 (SD: 14.3) out of 76 respectively. It significantly increased to 66.4 (SD: 8.0) and 71.1 (SD: 5.2) during the endline. The proportion of students and faculty found competent (achieved 75% in OSCE) also significantly increased from none at baseline to 91% and 98% respectively, at endline. CONCLUSION: The combination of attributes included in the PSE strengthening program may have contributed to improvements in institutional performance as well as MNH related competences of nursing midwifery students and faculty.

13.
Early Hum Dev ; 153: 105293, 2021 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-33340946

RESUMO

BACKGROUND: Right-hand preference is related to stronger right-directional asymmetry in the length of proximal upper-limb bones, although the relationships of hand preference with directional asymmetry in phalangeal bone lengths are not known. Furthermore, dorsal digit length is an easy-to-measure, faithful proxy of X-rayed phalangeal bone length (which is costly and difficult to measure). AIM: To study the effects of hand preference, sex, and age on right-left (R-L) asymmetry in dorsal digit lengths. METHODS: We measured all dorsal digit lengths (except the thumb) in comparable numbers of left-handers and right-handers in samples of adults (N = 151, age: M = 22.6 years, SD = 3.3) and children (N = 65, age: M = 5.0 years, SD = 1.0). RESULTS: Right-handers and adults had stronger right-directional asymmetry in digit lengths than left-handers and children. A Bayesian analysis yielded an 'extremely strong likelihood' of no sex differences in the R-L asymmetry of dorsal digit lengths 2 and 4. CONCLUSIONS: The effects of hand preference, sex, and age on R-L asymmetry appear to be similar for phalangeal bone length and other (proximal) upper-limb bone lengths. Two distinct biologic mechanisms (i.e., a general right-directional asymmetry mechanism and a handedness-related directional asymmetry mechanism) may contribute to observed R-L asymmetry in limbs. Fingertip fat and bone digit length do not seem to contribute to sex differences in the R-L asymmetry (Dr-l) of the widely studied second-to-fourth digit ratio (2D:4D).

14.
Bioresour Technol ; 319: 124235, 2021 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-33254459

RESUMO

The co-production of industrially relevant biopolymers/biomolecules from microbes is of biotechnological importance. Herein, a unique bacterium, Iodobacter sp. PCH 194 from the kettle lake at Sach Pass in western Indian Himalaya was identified. It co-produces biopolymer polyhydroxyalkanoates (PHA) and biomolecule (violacein pigment). Statistical optimization yielded dual products in the medium augmented with glucose (4.0% w/v) and tryptone (0.5% w/v) as carbon and nitrogen sources, respectively. The purified PHA was polyhydroxybutyrate (PHB), and pigment constitutes of violacein (50-60%) and deoxyviolacein (40-50%). A bench-scale bioprocess in 22.0 L fermentor with 20% dissolved O2 supply produced PHB (11.0 ± 1.0 g/L, 58% of dry cell mass) and violacein pigment (1.5 ± 0.08 g/L). PHB obtained was used for the preparation of bioplastic film. Violacein pigment experimentally validated for anticancerous and antimicrobial activities. In summary, a commercially implied bioprocess developed for the co-production of PHB and violacein pigment using the Himalayan bacterium.


Assuntos
Betaproteobacteria , Poli-Hidroxialcanoatos , Bactérias , Indóis
15.
Cancer Treat Res Commun ; 25: 100258, 2020 Dec 02.
Artigo em Inglês | MEDLINE | ID: mdl-33307507

RESUMO

The application of gold nanoparticles in immunotherapy has emerged as one of the most effective therapeutic strategy for eradicating cancer by releasing antigens, oligonucleotides, adjuvants, immune-stimulating agents into the body. Gold nanoparticles are found to be a superior choice, for generating attack on oncogenic cells, due to their low toxicity, better target specificity, diagnostic capabilities, and enhanced cellular uptake rate. This review focuses on the efficiency of several functionalized gold nanoparticles of diverse shapes and sizes as delivery vehicles to desired target cells through effective immunotherapy, along with a brief discussion about photothermal therapy.

16.
Phys Fluids (1994) ; 32(11): 111301, 2020 Nov 01.
Artigo em Inglês | MEDLINE | ID: mdl-33281434

RESUMO

In the unfortunate event of the current ongoing pandemic COVID-19, where vaccination development is still in the trial phase, several preventive control measures such as social distancing, hand-hygiene, and personal protective equipment have been recommended by health professionals and organizations. Among them, the safe wearing of facemasks has played a vital role in reducing the likelihood and severity of infectious respiratory disease transmission. The reported research in facemasks has covered many of their material types, fabrication techniques, mechanism characterization, and application aspects. However, in more recent times, the focus has shifted toward the theoretical investigations of fluid flow mechanisms involved in the virus-laden particles' prevention by using facemasks. This exciting research domain aims to address the complex fluid transport that led to designing a facemask with a better performance. This Review discusses the recent updates on fluid flow dynamics through the facemasks. Key design aspects such as thermal comfort and flow resistance are discussed. Furthermore, the recent progress in the investigations on the efficacy of facemasks for the prevention of COVID-19 spread and the impact of wearing facemasks is presented.

17.
iScience ; 23(12): 101770, 2020 Dec 18.
Artigo em Inglês | MEDLINE | ID: mdl-33294780

RESUMO

Tumor progression is profoundly influenced by interactions between cancer cells and the tumor microenvironment (TME). Among the various non-neoplastic cells present, immune cells are critical players in tumor development and have thus emerged as attractive therapeutic targets. Malignant gliomas exhibit a unique immune landscape characterized by high numbers of tumor-associated macrophages (TAMs). Despite encouraging preclinical results, targeting TAMs has yielded limited clinical success as a strategy for slowing glioma progression. The slow translational progress of TAM-targeted therapies is due in part to an incomplete understanding of the factors driving TAM recruitment, differentiation, and polarization. Furthermore, the functions that TAMs adopt in gliomas remain largely unknown. Progress in addressing these gaps requires sophisticated culture platforms capable of capturing key cellular and physical TME features. This review summarizes the current understanding of TAMs in gliomas and highlights the utility of in vitro TME models for investigating TAM-cancer cell cross talk.

18.
ISA Trans ; 2020 Dec 03.
Artigo em Inglês | MEDLINE | ID: mdl-33303226

RESUMO

Atangana-Baleanu-Caputo (ABC) fractional differential operator based upon Mittag-Leffler kernel exhibits all the advantages of conventional Riemann-Liouville and Caputo fractional differential operators; in addition, the kernel associated is non-singular. Therefore, this paper puts forward a closed-form analytical formulation for the design of an ABC-based fractional-order FIR filter for various signal processing and filtering applications. The closed-form expression is derived by utilizing backward finite difference method and fractional sample delay interpolation techniques. Furthermore, several design examples are considered to illustrate the effectiveness of the proposed method. From the analytical and simulation studies done, it is observed that the proposed design efficiently approximates the ideal frequency response of ABC-fractional differential operator. Finally, one-dimensional and two-dimensional applications of the proposed method are validated and compared against state-of-the-art methods for electrocardiogram (ECG) R-peak detection as well as for digital image sharpening.

19.
Data Brief ; 33: 106566, 2020 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-33304963

RESUMO

The global crisis prevailing in the wake of the spread of COVID-19 has raised several speculations about the impact of the lockdown on the mental health of people. The dataset presented here is the assessment of the psychological distress experienced by people in India following the implementation of lockdown as a measure to curtail the spread of the coronavirus. The data was collected through a survey conducted by employing an online questionnaire assessing the socio-demographic information (9-items) as well as the administration of the short version of the General Health Questionnaire (GHQ-12 items) originally developed by Goldberg (1972). The period of data collection is between 9th April 2020 and 20th April 2020 where a total of 1,894 responses were obtained. The Google forms containing the questionnaire of the study were shared publicly through emails and via the social media forum like WhatsApp and Facebook. Thereby, those who took the initiative to fill-up the responses were included as the survey participants. Thus, the final sample had participants representing 17 states and Union territories of India. The entire dataset is stored in a Microsoft Excel Worksheet (.xls) and the questionnaire is attached as a supplementary file. The data is beneficial for the timely assessment of the nature and degree of the psychological distress experienced by people in India during the COVID-19 crisis. It could further be an assistance to the Government, policymakers as well as health care workers to take the adequate measures to ensure sound mental health among people.

20.
BMJ Case Rep ; 13(12)2020 Dec 22.
Artigo em Inglês | MEDLINE | ID: mdl-33370975

RESUMO

A 52-year-old man presented with lower urinary tract symptoms and intermittent haematuria for the last 6 months. He had undergone totally extraperitoneal right inguinal hernia repair a decade ago. The ultrasonography and an X-ray of the pelvis suggested a large radio-opaque shadow in the bladder. However, CT revealed an encrusted intravesical extension of the migrated mesh along the right anterolateral wall. The entire intravesical part of the migrated mesh with encrustations was successfully retrieved by endourological approach using holmium laser. The patient symptomatically improved and at follow-up, cystoscopy showed a complete re-epithelisation of the bladder mucosa. The intravesical extension of migrated mesh is a rare but challenging complication following mesh hernioplasty and can be successfully managed with a complete endoscopic approach.


Assuntos
Remoção de Dispositivo/métodos , Migração de Corpo Estranho/cirurgia , Herniorrafia/efeitos adversos , Lasers de Estado Sólido/uso terapêutico , Falha de Prótese , Telas Cirúrgicas/efeitos adversos , Cistoscopia , Remoção de Dispositivo/instrumentação , Migração de Corpo Estranho/complicações , Migração de Corpo Estranho/diagnóstico , Hematúria/diagnóstico , Hematúria/etiologia , Hematúria/cirurgia , Hérnia Inguinal/cirurgia , Herniorrafia/instrumentação , Humanos , Sintomas do Trato Urinário Inferior/diagnóstico , Sintomas do Trato Urinário Inferior/etiologia , Sintomas do Trato Urinário Inferior/cirurgia , Masculino , Pessoa de Meia-Idade , Tomografia Computadorizada por Raios X , Ultrassonografia , Bexiga Urinária/diagnóstico por imagem , Bexiga Urinária/cirurgia
SELEÇÃO DE REFERÊNCIAS
DETALHE DA PESQUISA
...