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1.
Einstein (Säo Paulo) ; 18: eAO4876, 2020. tab, graf
Artigo em Inglês | LILACS-Express | ID: biblio-1039734

RESUMO

ABSTRACT Objective To investigate the effects of sericin extracted from silkworm Bombyx mori cocoon on morphophysiological parameters in mice with obesity induced by high-fat diet. Methods Male C57Bl6 mice aged 9 weeks were allocated to one of two groups - Control and Obese, and fed a standard or high-fat diet for 10 weeks, respectively. Mice were then further subdivided into four groups with seven mice each, as follows: Control, Control-Sericin, Obese, and Obese-Sericin. The standard or high fat diet was given for 4 more weeks; sericin (1,000mg/kg body weight) was given orally to mice in the Control-Sericin and Obese-Sericin Groups during this period. Weight gain, food intake, fecal weight, fecal lipid content, gut motility and glucose tolerance were monitored. At the end of experimental period, plasma was collected for biochemical analysis. Samples of white adipose tissue, liver and jejunum were collected and processed for light microscopy analysis; liver fragments were used for lipid content determination. Results Obese mice experienced significantly greater weight gain and fat accumulation and had higher total cholesterol and glucose levels compared to controls. Retroperitoneal and periepididymal adipocyte hypertrophy, development of hepatic steatosis, increased cholesterol and triglyceride levels and morphometric changes in the jejunal wall were observed. Conclusion Physiological changes induced by obesity were not fully reverted by sericin; however, sericin treatment restored jejunal morphometry and increased lipid excretion in feces in obese mice, suggesting potential anti-obesity effects.


RESUMO Objetivo Investigar os efeitos da sericina extraída de casulos de Bombyx mori na morfofisiologia de camundongos com obesidade induzida por dieta hiperlipídica. Métodos Camundongos machos C57Bl6, com 9 semanas de idade, foram distribuídos em Grupos Controle e Obeso, que receberam ração padrão para roedores ou dieta hiperlipídica por 10 semanas, respectivamente. Posteriormente, os animais foram redistribuídos em quatro grupos, com sete animais cada: Controle, Controle-Sericina, Obeso e Obeso-Sericina. Os animais permaneceram recebendo ração padrão ou hiperlipídica por 4 semanas, período no qual a sericina foi administrada oralmente na dose de 1.000mg/kg de massa corporal aos Grupos Controle-Sericina e Obeso-Sericina. Parâmetros fisiológicos, como ganho de peso, consumo alimentar, peso das fezes em análise de lipídios fecais, motilidade intestinal e tolerância à glicose foram monitorados. Ao término do experimento, o plasma foi coletado para dosagens bioquímicas e fragmentos de tecido adiposo branco; fígado e jejuno foram processados para análises histológicas, e amostras hepáticas foram usadas para determinação lipídica. Resultados Camundongos obesos apresentaram ganho de peso e acúmulo de gordura significativamente maior que os controles, aumento do colesterol total e glicemia. Houve hipertrofia dos adipócitos retroperitoneais e periepididimais, instalação de esteatose e aumento do colesterol e triglicerídeos hepáticos, bem como alteração morfométrica da parede jejunal. Conclusão O tratamento com sericina não reverteu todas as alterações fisiológicas promovidas pela obesidade, mas restaurou a morfometria jejunal e aumentou a quantidade de lipídios eliminados nas fezes dos camundongos obesos, apresentando-se como potencial tratamento para a obesidade.

2.
Einstein (Sao Paulo) ; 18: eAO4876, 2020.
Artigo em Inglês, Português | MEDLINE | ID: mdl-31576909

RESUMO

OBJECTIVE: To investigate the effects of sericin extracted from silkworm Bombyx mori cocoon on morphophysiological parameters in mice with obesity induced by high-fat diet. METHODS: Male C57Bl6 mice aged 9 weeks were allocated to one of two groups - Control and Obese, and fed a standard or high-fat diet for 10 weeks, respectively. Mice were then further subdivided into four groups with seven mice each, as follows: Control, Control-Sericin, Obese, and Obese-Sericin. The standard or high fat diet was given for 4 more weeks; sericin (1,000mg/kg body weight) was given orally to mice in the Control-Sericin and Obese-Sericin Groups during this period. Weight gain, food intake, fecal weight, fecal lipid content, gut motility and glucose tolerance were monitored. At the end of experimental period, plasma was collected for biochemical analysis. Samples of white adipose tissue, liver and jejunum were collected and processed for light microscopy analysis; liver fragments were used for lipid content determination. RESULTS: Obese mice experienced significantly greater weight gain and fat accumulation and had higher total cholesterol and glucose levels compared to controls. Retroperitoneal and periepididymal adipocyte hypertrophy, development of hepatic steatosis, increased cholesterol and triglyceride levels and morphometric changes in the jejunal wall were observed. CONCLUSION: Physiological changes induced by obesity were not fully reverted by sericin; however, sericin treatment restored jejunal morphometry and increased lipid excretion in feces in obese mice, suggesting potential anti-obesity effects.


Assuntos
Fármacos Antiobesidade/uso terapêutico , Dieta Hiperlipídica , Obesidade/tratamento farmacológico , Sericinas/uso terapêutico , Tecido Adiposo/patologia , Animais , Fármacos Antiobesidade/farmacologia , Peso Corporal/efeitos dos fármacos , Colesterol/análise , Dieta Hiperlipídica/efeitos adversos , Ingestão de Alimentos/efeitos dos fármacos , Fígado Gorduroso/patologia , Trânsito Gastrointestinal/efeitos dos fármacos , Teste de Tolerância a Glucose , Fígado/metabolismo , Masculino , Camundongos Endogâmicos C57BL , Camundongos Obesos , Obesidade/etiologia , Obesidade/fisiopatologia , Reprodutibilidade dos Testes , Sericinas/farmacologia , Fatores de Tempo , Resultado do Tratamento , Triglicerídeos/análise , Ganho de Peso/efeitos dos fármacos
3.
Einstein (Sao Paulo) ; 16(1): eAO4137, 2018.
Artigo em Português, Inglês | MEDLINE | ID: mdl-29694624

RESUMO

Objective To analyze the combined effects of the silk protein sericin and swimming exercise on histomorphometry of the plantar muscle in Wistar rats. Methods Forty adult rats were randomly allocated into 5 groups comprising 8 animals each, as follows: Control, Injury, Sericin, Swim, and Swim plus Sericin. Three days after crushing of the sciatic nerve the rats in the Swim and Swim plus Sericin Groups were submitted to swimming exercise for 21 days. Rats were then euthanized and the plantar muscle harvested and processed. Results Cross-sectional area, peripheral nuclei and muscle fiber counts, nucleus/fiber ratio and smallest muscle fiber width did not differ significantly between groups. Morphological analysis revealed hypertrophic fibers in the Swim Group and evident muscle damage in the Swim plus Sericin and Injury Groups. The percentage of intramuscular collagen was apparently maintained in the Swim Group compared to remaining groups. Conclusion Combined treatment with sericin and swimming exercise did not improve muscle properties. However, physical exercise alone was effective in maintaining intramuscular connective tissue and preventing progression of deleterious effects of peripheral nerve injury.


Assuntos
Extremidade Inferior/inervação , Músculo Esquelético/inervação , Condicionamento Físico Animal/fisiologia , Sericinas/farmacologia , Natação/fisiologia , Animais , Modelos Animais de Doenças , Extremidade Inferior/lesões , Extremidade Inferior/patologia , Músculo Esquelético/lesões , Músculo Esquelético/patologia , Compressão Nervosa , Distribuição Aleatória , Ratos , Ratos Wistar
4.
J Exerc Rehabil ; 14(1): 24-31, 2018 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-29511649

RESUMO

Physical exercise may help maintain muscle properties and functional improvement after peripheral nerve lesion, which may be enhanced by using biocompatible substances, such as sericin. The aim of this study was analyse the effect of sericin associated with swimming exercise on the phenotype, innervation, and functionality of the plantar muscle of Wistar rats. Forty randomly divided adult rats were used in five groups of eight animals: control, injury, sericin, exercise, exercise and sericin. The application of sericin was done on the spot, 100 µL, shortly after nerve compression. Three days after sciatic nerve compression, the swimming and swimming and sericin groups were submitted to physical swimming exercise for 21 days. Afterwards, the animals were euthanised and the plantar muscle was dissected and submitted to histochemical and histoenzymological techniques. The grip strength test did not show alterations in muscular functionality, and the control presented greater muscle mass in relation to the other groups, the same did not occur for muscle length. Polymorphic neuromuscular junctions were detected in the groups, although without significant morphometric alterations of the area, major and minor diameters. The percentage of type I fibres was lower in the lesion group and there was no difference in fibres IIa and IIb between groups. The area of fibres I, IIa and IIb remained constant between groups. Sericin biopolymer combined with swimming exercise did not affect plantar muscle function, submitted to experimental axonotmosis, whose contractile properties were altered by nerve injury.

5.
Einstein (Säo Paulo) ; 16(1): eAO4137, 2018. tab, graf
Artigo em Inglês | LILACS-Express | ID: biblio-891463

RESUMO

Abstract Objective To analyze the combined effects of the silk protein sericin and swimming exercise on histomorphometry of the plantar muscle in Wistar rats. Methods Forty adult rats were randomly allocated into 5 groups comprising 8 animals each, as follows: Control, Injury, Sericin, Swim, and Swim plus Sericin. Three days after crushing of the sciatic nerve the rats in the Swim and Swim plus Sericin Groups were submitted to swimming exercise for 21 days. Rats were then euthanized and the plantar muscle harvested and processed. Results Cross-sectional area, peripheral nuclei and muscle fiber counts, nucleus/fiber ratio and smallest muscle fiber width did not differ significantly between groups. Morphological analysis revealed hypertrophic fibers in the Swim Group and evident muscle damage in the Swim plus Sericin and Injury Groups. The percentage of intramuscular collagen was apparently maintained in the Swim Group compared to remaining groups. Conclusion Combined treatment with sericin and swimming exercise did not improve muscle properties. However, physical exercise alone was effective in maintaining intramuscular connective tissue and preventing progression of deleterious effects of peripheral nerve injury.


RESUMO Objetivo Analisar o efeito da proteína sericina associada ao exercício físico de natação na histomorfometria do músculo plantar de ratos Wistar. Métodos Foram utilizados 40 ratos adultos divididos aleatoriamente em 5 grupos, com 8 animais cada: Controle, Lesão, Sericina, Natação, Natação e Sericina. Três dias após a compressão do nervo isquiático, os Grupos Natação e Exercício e Sericina foram submetidos ao exercício físico de natação durante 21 dias. Após, os animais foram sacrificados, e o músculo plantar foi processado. Resultados Não houve diferença da área da secção transversa entre os grupos, quantidade de núcleos periféricos, quantidade de fibra, relação núcleo/fibra e diâmetro menor. A análise morfológica revelou que no Grupo Natação ocorreu hipertrofia das fibras, assim como nos Grupos Exercício e Sericina e Lesão, o dano muscular foi evidente. O percentual de conjuntivo intramuscular parece ter sido mantido no Grupo Exercício em relação aos demais grupos. Conclusão A associação da proteína sericina e exercício físico de natação não foi eficiente na melhora das propriedades musculares, embora a aplicação do exercício físico tenha sido eficiente na manutenção do conjuntivo intramuscular, e no não agravamento dos efeitos deletérios consequentes da lesão nervosa periférica.

6.
Einstein (Sao Paulo) ; 15(2): 186-191, 2017.
Artigo em Inglês, Português | MEDLINE | ID: mdl-28767917

RESUMO

Objective: To evaluate the action of vanillin (Vanilla planifolia) on the morphology of tibialis anterior and soleus muscles after peripheral nerve injury. Methods: Wistar rats were divided into four groups, with seven animals each: Control Group, Vanillin Group, Injury Group, and Injury + Vanillin Group. The Injury Group and the Injury + Vanillin Group animals were submitted to nerve injury by compression of the sciatic nerve; the Vanillin Group and Injury + Vanillin Group, were treated daily with oral doses of vanillin (150mg/kg) from the 3rd to the 21st day after induction of nerve injury. At the end of the experiment, the tibialis anterior and soleus muscles were dissected and processed for light microscopy and submitted to morphological analysis. Results: The nerve compression promoted morphological changes, typical of denervation, and the treatment with vanillin was responsible for different responses in the studied muscles. For the tibialis anterior, there was an increase in the number of satellite cells, central nuclei and fiber atrophy, as well as fascicular disorganization. In the soleus, only increased vascularization was observed, with no exacerbation of the morphological alterations in the fibers. Conclusion: The treatment with vanillin promoted increase in intramuscular vascularization for the muscles studied, with pro-inflammatory potential for tibialis anterior, but not for soleus muscle. Objetivo: Avaliar a ação da vanilina (Vanilla planifolia) sobre a morfologia dos músculos tibial anterior e sóleo após lesão nervosa periférica. Métodos: Ratos Wistar foram divididos em quatro grupos, com sete animais cada, sendo Grupo Controle, Grupo Vanilina, Grupo Lesão e Grupo Lesão + Vanilina. Os animais dos Grupos Lesão e Grupo Lesão + Vanilina foram submetidos à lesão nervosa por meio da compressão do nervo isquiático, e os Grupos Vanilina e Grupo Lesão + Vanilina foram tratados diariamente com doses orais de vanilina (150mg/kg) do 3o ao 21o dia após a indução da lesão nervosa. Ao término do experimento, os músculos tibial anterior e sóleo foram dissecados e seguiram o processamento de rotina em microscopia de luz, para posterior análise morfológica. Resultados: A compressão nervosa promoveu alterações morfológicas características de denervação, sendo que o tratamento com vanilina foi responsável por respostas distintas nos músculos estudados. Para o tibial anterior, houve aumento do número de células satélites, núcleos centrais e atrofia das fibras, bem como desorganização fascicular. Já no sóleo, houve apenas aumento da vascularização, sem exacerbação das alterações morfológicas nas fibras. Conclusão: O tratamento com vanilina promoveu o aumento da vascularização intramuscular para os músculos estudados, com potencial pró-inflamatório para o tibial anterior, o que não ocorreu no músculo sóleo.


Assuntos
Anti-Inflamatórios/farmacologia , Benzaldeídos/farmacologia , Tecido Conjuntivo/efeitos dos fármacos , Músculo Esquelético/efeitos dos fármacos , Neuropatia Ciática/patologia , Animais , Tecido Conjuntivo/patologia , Humanos , Masculino , Modelos Animais , Fibras Musculares Esqueléticas/efeitos dos fármacos , Músculo Esquelético/patologia , Distribuição Aleatória , Ratos Wistar , Neuropatia Ciática/reabilitação
7.
Einstein (Säo Paulo) ; 15(2): 186-191, Apr.-June 2017. tab, graf
Artigo em Inglês | LILACS | ID: biblio-891383

RESUMO

ABSTRACT Objective To evaluate the action of vanillin (Vanilla planifolia) on the morphology of tibialis anterior and soleus muscles after peripheral nerve injury. Methods Wistar rats were divided into four groups, with seven animals each: Control Group, Vanillin Group, Injury Group, and Injury + Vanillin Group. The Injury Group and the Injury + Vanillin Group animals were submitted to nerve injury by compression of the sciatic nerve; the Vanillin Group and Injury + Vanillin Group, were treated daily with oral doses of vanillin (150mg/kg) from the 3rd to the 21st day after induction of nerve injury. At the end of the experiment, the tibialis anterior and soleus muscles were dissected and processed for light microscopy and submitted to morphological analysis. Results The nerve compression promoted morphological changes, typical of denervation, and the treatment with vanillin was responsible for different responses in the studied muscles. For the tibialis anterior, there was an increase in the number of satellite cells, central nuclei and fiber atrophy, as well as fascicular disorganization. In the soleus, only increased vascularization was observed, with no exacerbation of the morphological alterations in the fibers. Conclusion The treatment with vanillin promoted increase in intramuscular vascularization for the muscles studied, with pro-inflammatory potential for tibialis anterior, but not for soleus muscle.


RESUMO Objetivo Avaliar a ação da vanilina (Vanilla planifolia) sobre a morfologia dos músculos tibial anterior e sóleo após lesão nervosa periférica. Métodos Ratos Wistar foram divididos em quatro grupos, com sete animais cada, sendo Grupo Controle, Grupo Vanilina, Grupo Lesão e Grupo Lesão + Vanilina. Os animais dos Grupos Lesão e Grupo Lesão + Vanilina foram submetidos à lesão nervosa por meio da compressão do nervo isquiático, e os Grupos Vanilina e Grupo Lesão + Vanilina foram tratados diariamente com doses orais de vanilina (150mg/kg) do 3o ao 21o dia após a indução da lesão nervosa. Ao término do experimento, os músculos tibial anterior e sóleo foram dissecados e seguiram o processamento de rotina em microscopia de luz, para posterior análise morfológica. Resultados A compressão nervosa promoveu alterações morfológicas características de denervação, sendo que o tratamento com vanilina foi responsável por respostas distintas nos músculos estudados. Para o tibial anterior, houve aumento do número de células satélites, núcleos centrais e atrofia das fibras, bem como desorganização fascicular. Já no sóleo, houve apenas aumento da vascularização, sem exacerbação das alterações morfológicas nas fibras. Conclusão O tratamento com vanilina promoveu o aumento da vascularização intramuscular para os músculos estudados, com potencial pró-inflamatório para o tibial anterior, o que não ocorreu no músculo sóleo.


Assuntos
Humanos , Animais , Masculino , Benzaldeídos/farmacologia , Músculo Esquelético/efeitos dos fármacos , Tecido Conjuntivo/efeitos dos fármacos , Neuropatia Ciática/patologia , Anti-Inflamatórios/farmacologia , Distribuição Aleatória , Ratos Wistar , Músculo Esquelético/patologia , Fibras Musculares Esqueléticas/efeitos dos fármacos , Tecido Conjuntivo/patologia , Neuropatia Ciática/reabilitação , Modelos Animais
8.
Motriz (Online) ; 23(3): e101707, 2017. tab, ilus
Artigo em Inglês | LILACS | ID: biblio-894990

RESUMO

Aim: to determine the effects of sciatic compression and treatment with resistance exercise on the morphology of the ankle joint of Wistar rats. Methods: 32 male rats, aged 10 ± 1 week, weighing 376±22 grams were divided into the following four groups (n=8/group): CG (control), LG (lesion), EG (exercise) and LEG (lesion and exercise). Three days after sciatic compression, the animals in the EG and LEG were submitted to resistance exercise by climbing stairs (five days/week) for three weeks and a load of 100 grams was added. The exercise was carried out in two sets of ten consecutive ascents of the steps. The ankle joint tissues were analyzed for their morphometry and morphology using light microscopy. Results: Regarding the number of chondrocytes, the LG and EG had more cells in the anterior articular cartilage in the tibia (62 and 43%) and in the talus (57 and 45%) when compared to the CG. In the LEG there was a 25% and 26% reduction of chondrocytes in the anterior cartilage in the tibia and talus when compared to the LG. Changes were observed in the tibia and talus in the LG, with the presence of flocculation, invagination of the subchondral bone, discontinuity of tidemark and pannus covering the subchondral bone in the talus, as well as changes in the synovial membrane. These alterations were minimized in the articular cartilage and synovial membrane in the LEG. Conclusions exercise restores the tissue morphology of ankle joint in Wistar rats after sciatic compression.(AU)


Assuntos
Animais , Masculino , Ratos , Membrana Sinovial , Cartilagem Articular , Treinamento de Resistência , Articulação do Tornozelo , Ratos Wistar
9.
Biomed Res Int ; 2016: 8175701, 2016.
Artigo em Inglês | MEDLINE | ID: mdl-27965981

RESUMO

Silk sericin is a natural polymer produced by silkworm, Bombyx mori, which surrounds and keeps together two fibroin filaments in silk thread used in the cocoon. The recovery and reuse of sericin usually discarded by the textile industry not only minimizes environmental issues but also has a high scientific and commercial value. The physicochemical properties of the molecule are responsible for numerous applications in biomedicine and are influenced by the extraction method and silkworm lineage, which can lead to variations in molecular weight and amino acid concentration of sericin. The presence of highly hydrophobic amino acids and its antioxidant potential make it possible for sericin to be applied in the food and cosmetic industry. The moisturizing power allows indications as a therapeutic agent for wound healing, stimulating cell proliferation, protection against ultraviolet radiation, and formulating creams and shampoos. The antioxidant activity associated with low digestibility of sericin that expands the application in the medical field, such as antitumour, antimicrobial and anti-inflammatory agent, anticoagulant, acts in colon health, improving constipation and protects the body from obesity through improved plasma lipid profile. In addition, the properties of sericin allow its application as a culture medium and cryopreservation, in tissue engineering and for drug delivery, demonstrating its effective use, as an important biomaterial.


Assuntos
Tecnologia Biomédica/métodos , Bombyx/química , Sericinas/metabolismo , Estruturas Animais/anatomia & histologia , Animais , Sistemas de Liberação de Medicamentos , Humanos , Sericinas/biossíntese , Engenharia Tecidual
10.
Einstein (Säo Paulo) ; 13(4): 574-579, Oct.-Dec. 2015. graf
Artigo em Português | LILACS | ID: lil-770488

RESUMO

ABSTRACT Objective To analyze the effects of physical exercise on cartilage histomorphometry in osteoporosis-induced rats subjected to immobilization. Methods We used 36 Wistar rats that were separated into six groups: G1, G2 and G3 submitted to pseudo-oophorectomy, and G4, G5 and G6 submitted to oophorectomy. After 60 days at rest, G2, G3, G5 and G6 had the right hind limbs immobilized for 15 days, followed by the same period in remobilization, being free in the box to G2 and G5, and climb ladder to G3 and G6. At the end of the experiment, the rats were euthanized, their tibias bilaterally removed and submitted to histological routine. Results There was significant increase in thickness of the articular cartilage (F(5;29)=13.88; p<0.0001) and epiphyseal plate (F(5;29)=14.72; p<0.0001) as the number of chondrocytes (F(5;29)=5.11; p=0.0021) in ovariectomized rats, immobilized and submitted to exercise. In the morphological analysis, degeneration of articular cartilage with subchondral bone exposure, loss of cellular organization, discontinuity of tidemark, presence of cracks and flocculation in ovariectomized, immobilized and free remobilization rats were found. In ovariectomized and immobilized remobilization ladder rats, signs of repair of the cartilaginous structures in the presence of clones, pannus, subcortical blood vessel invasion in the calcified zone, increasing the amount of isogenous groups and thickness of the calcified zone were observed. Conclusion Exercise climb ladder was effective in cartilaginous tissue recovery process damaged by immobilization, in model of osteoporosis by ovariectomy in rats.


RESUMO Objetivo Analisar os efeitos do exercício físico sobre a histomorfometria da cartilagem de ratas induzidas à osteoporose e submetidas à imobilização. Métodos Foram utilizadas 36 ratas Wistar separadas em seis grupos: G1, G2 e G3 submetidas à pseudo-ooforectomia e G4, G5 e G6, à ooforectomia. Após 60 dias em repouso, G2, G3, G5 e G6 tiveram o membro posterior direito imobilizado por 15 dias, seguido pelo mesmo período em remobilização, sendo livres na caixa para G2 e G5, e de subida em escada para G3 e G6. Ao final do experimento, as ratas foram eutanasiadas, e suas tíbias foram retiradas bilateralmente e submetidas à rotina histológica. Resultados Houve aumento significativo de espessura da cartilagem articular (F(5;29)=13,88; p<0,0001) e da placa epifisária (F(5;29)=14,72; p<0,0001), bem como do número de condrócitos (F(5;29)=5,11; p<0,0021) em ratas ooforectomizadas, imobilizadas e submetidas ao exercício em escada. Nas análises morfológicas, verificaram-se degeneração da cartilagem articular com exposição de osso subcondral, perda da organização celular, descontinuidade da tidemark, presença de fissuras e floculações em ratas ooforectomizadas, imobilizadas e com remobilização livre. Nas ratas ooforectomizadas, imobilizadas e com remobilização em escada, observaram-se sinais de reparação das estruturas cartilaginosas com presença de clones, pannus, invasão de vasos sanguíneos subcorticais na zona calcificada, aumento da quantidade de grupos isógenos e espessura da zona calcificada. Conclusão O exercício físico de subida em escada mostrou-se efetivo no processo de recuperação do tecido cartilaginoso danificado pela imobilização, em modelo de osteoporose por ooforectomia em ratas.


Assuntos
Animais , Feminino , Cartilagem Articular/patologia , Terapia por Exercício/métodos , Imobilização/efeitos adversos , Atividade Motora/fisiologia , Osteoporose/terapia , Tíbia/patologia , Contagem de Células , Cartilagem Articular/metabolismo , Condrócitos/patologia , Modelos Animais de Doenças , Lâmina de Crescimento/metabolismo , Ovariectomia , Osteoporose/etiologia , Osteoporose/patologia , Ratos Wistar
11.
Fisioter. pesqui ; 22(3): 317-324, jul.-set. 2015. tab, graf
Artigo em Inglês | LILACS-Express | ID: lil-767875

RESUMO

Resumo O objetivo deste estudo foi verificar os efeitos da imobilização e analisar a influência da remobilização livre ou por associação de exercícios terapêuticos em meio aquático, sobre a morfologia da articulação do joelho. Foram utilizados 18 ratos Wistar que tiveram seu membro posterior direito imobilizado em extensão completa do joelho por 15 dias e que foram divididos igualmente em três grupos: G1 não sofreu nenhuma intervenção; G2 foi submetido à remobilização livre; e G3 teve remobilização por exercícios (natação e salto) em meio aquático por 14 dias. As articulações do joelho, direitas e esquerdas, foram coletadas e incluídas em parafina para análise morfológica. A imobilização e remobilização alteraram a espessura da cartilagem articular e o número de condrócitos. A cartilagem articular e a membrana sinovial sofreram mudanças degenerativas devido ao desuso articular na imobilização e estas mudanças foram progressivamente revertidas pela remobilização em meio aquático. A combinação de natação e salto em meio aquático se mostraram uma alternativa eficiente de remobilização articular, podendo ser utilizada como exercício em programa de reabilitação fisioterapêutica.


Resumen El objetivo de este estudio fue verificar los efectos de la inmovilización y analizar la influencia de la removilización libre o por asociación de ejercicios terapéuticos en medio acuático, sobre la morfología de la articulación de la rodilla. Se utilizó 18 ratones Wistar, que tuvieron sus miembros superiores derechos inmovilizados en extensión completa de la rodilla, por 15 días, y divididos igualmente en tres grupos: G1, no ha sufrido ninguna intervención; G2, fue sometido a removilización libre; y G3, removilización por ejercicios (natación y salto) en medio acuático por 14 días. Las articulaciones de la rodilla, derechas e izquierdas, fueron colectadas e incluidas en parafina para análisis morfológica. La inmovilización y removilización alteraron el espesor del cartílago articular y el número de condrocitos. El cartílago articular y la membrana sinovial sufrieron cambios degenerativos, debido al desuso articular en la inmovilización; estes cambios fueron progresivamente revertidos por la inmovilización en medio acuático. La combinación de natación y salto en medio acuático se mostró una alternativa eficiente de removilización articular, pudiendo ser utilizados como ejercicios en programas de rehabilitación fisioterapéutica.


Abstract The objective of this study was to investigate the effects of immobilization and analyze the influence of free remobilization or the association of therapeutic exercises in an aquatic environment on the morphology of the knee joint. Eighteen Wistar rats were used. They had had their right hind limb immobilized at the full extension of the knee for 15 days, and also divided into three groups: G1, which suffered no intervention; G2, which were subjected to free remobilization; and G3, which had remobilization through exercises (swimming and jumping) in an aquatic environment for 14 days. The right and left knee articulations were collected and embedded in paraffin for morphological analysis. The immobilization and remobilization altered the thickness of the articular cartilage and the number of chondrocytes. The articular cartilage and the synovial membrane suffer degenerative changes due to articular disuse during immobilization; and these changes were gradually reversed by remobilization in an aquatic environment. The combination of swimming and jumping in an aquatic environment proved to be an efficient alternative to articular remobilization and can be used as exercises in physiotherapy rehabilitation programs.

12.
Rev. bras. reumatol ; 55(1): 43-47, Jan-Feb/2015. tab
Artigo em Português | LILACS | ID: lil-744671

RESUMO

Objetivo Verificar a funcionalidade por meio da força muscular de preensão em animais com obesidade induzida por glutamato monossódico (MSG) e animais controle, que sofreram compressão do nervo mediano direito, tendo como tratamento a natação com carga. Métodos Ratos Wistar neonatos durante os primeiros cinco dias de vida receberam injeções subcutâneas de MSG. O grupo controle recebeu solução salina hiperosmótica. Quarenta e oito ratos foram divididos em seis grupos: G1(controle); G2 (controle com lesão); G3 (controle com lesão + natação); G4 (obesos); G5 (obesos com lesão); G6 (obesos com lesão + natação). Os animais dos grupos G2, G3, G5 e G6 foram submetidos à compressão do nervo mediano e os dos grupos G3 e G6 foram tratados, após a lesão, com exercício de natação com carga durante três semanas. A natação teve duração progressiva conforme as semanas, de 20, 30 e 40 minutos. A força muscular foi avaliada por meio de um medidor de força de preensão no pré-operatório, no terceiro, sétimo, 14° e 21° dia pós-operatório. Os resultados foram expressos e analisados por estatística descritiva e inferencial. Resultados Quando comparada a força de preensão entre as avaliações, indiferentemente de grupos, na segunda avaliação os animais apresentaram menor força de preensão. Os grupos G1 e G4 apresentaram força de preensão maior, em comparação com os grupos G2, G3, G4 e G6. Conclusão O exercício de natação com sobrecarga não foi eficaz em promover melhoria na força muscular de preensão após lesão de compressão do nervo mediano direito em ratos controle e obesos-MSG. .


Objective To verify the functionality through muscle grip strength in animals with obesity induced by monosodium glutamate (MSG) and in control animals, which suffered compression of the right median nerve, and treated with swimming with overload. Methods During the first five days of life, neonatal Wistar rats received subcutaneous injections of MSG. The control group received a hypertonic saline solution. Forty-eight rats were divided into six groups: G1 (control); G2 (control + injury); G3 (control + injury + swimming); G4 (obese); G5 (obese + injury); and G6 (obese + injury + swimming). The animals in groups G2, G3, G5 and G6 were submitted to compression of the median nerve and G3 and G6 groups were treated, after injury, with swimming exercise with load for three weeks. The swimming exercise had a progressive duration, according to the week, of 20, 30 and 40 min. Muscle strength was assessed using a grip strength meter preoperatively and on the 3rd, 7th, 14th and 21st days after surgery. The results were expressed and analyzed using descriptive and inferential statistics. Results When the grip strength was compared among assessments regardless of group, in the second assessment the animals exhibited lower grip strength. G1 and G4 groups had greater grip strength, compared to G2, G3, G4 and G6. Conclusion The swimming exercise with overload has not been effective in promoting improvement in muscle grip strength after compression injury of the right median nerve in control and in obese-MSG rats. .


Assuntos
Animais , Ratos , Força da Mão , Síndromes de Compressão Nervosa/fisiopatologia , Obesidade/fisiopatologia , Natação , Nervo Mediano , Síndromes de Compressão Nervosa/complicações , Obesidade/complicações , Ratos Wistar , Suporte de Carga
13.
Neurol Res ; 37(12): 1118-24, 2015.
Artigo em Inglês | MEDLINE | ID: mdl-26923582

RESUMO

OBJECTIVES: We, herein, analyzed the effect of swimming on nociception threshold and peripheral nerve regeneration in lean and obese rats submitted to median nerve compression. METHODS: To induce obesity, newborn male Wistar rats received injections of monosodium glutamate (MSG), whereas the control (CTL) group received saline. The animals were separated into 6 groups; control and obese (CTL and MSG), control and obese with lesion (CTL LES and MSG LES), and control and obese with lesion submitted to physical exercise (CTL LES PE and MSG LES PE). RESULTS: Median nerve compression reduced nociception threshold in CTL LES and MSG LES rats. Swimming effectively altered nociception only in CTL LES PE animals. Lean and obese animals displayed histological differences, when compared to sedentary animals, and exercise improved axon regeneration in both groups. The brain-derived neurotrophic factor and GAP 43 protein expression was greater in animals submitted to nervous compression without alteration by exercise. DISCUSSION: In conclusion, swimming, a conservative treatment for peripheral nerve lesions, was not able to improve the nociception threshold in obese rats.


Assuntos
Síndromes de Compressão Nervosa/fisiopatologia , Síndromes de Compressão Nervosa/reabilitação , Nociceptividade/fisiologia , Obesidade/complicações , Limiar da Dor/fisiologia , Natação , Análise de Variância , Animais , Animais Recém-Nascidos , Índice de Massa Corporal , Fator Neurotrófico Derivado do Encéfalo/metabolismo , Modelos Animais de Doenças , Proteína GAP-43/metabolismo , Regulação da Expressão Gênica/efeitos dos fármacos , Regulação da Expressão Gênica/fisiologia , Masculino , Nervo Mediano/fisiopatologia , Síndromes de Compressão Nervosa/patologia , Obesidade/induzido quimicamente , Medição da Dor , Condicionamento Físico Animal , Ratos , Ratos Wistar , Glutamato de Sódio/farmacologia
14.
Einstein (Sao Paulo) ; 13(4): 574-9, 2015.
Artigo em Inglês, Português | MEDLINE | ID: mdl-26761556

RESUMO

OBJECTIVE: To analyze the effects of physical exercise on cartilage histomorphometry in osteoporosis-induced rats subjected to immobilization. METHODS: We used 36 Wistar rats that were separated into six groups: G1, G2 and G3 submitted to pseudo-oophorectomy, and G4, G5 and G6 submitted to oophorectomy. After 60 days at rest, G2, G3, G5 and G6 had the right hind limbs immobilized for 15 days, followed by the same period in remobilization, being free in the box to G2 and G5, and climb ladder to G3 and G6. At the end of the experiment, the rats were euthanized, their tibias bilaterally removed and submitted to histological routine. RESULTS: There was significant increase in thickness of the articular cartilage (F(5;29)=13.88; p<0.0001) and epiphyseal plate (F(5;29)=14.72; p<0.0001) as the number of chondrocytes (F(5;29)=5.11; p=0.0021) in ovariectomized rats, immobilized and submitted to exercise. In the morphological analysis, degeneration of articular cartilage with subchondral bone exposure, loss of cellular organization, discontinuity of tidemark, presence of cracks and flocculation in ovariectomized, immobilized and free remobilization rats were found. In ovariectomized and immobilized remobilization ladder rats, signs of repair of the cartilaginous structures in the presence of clones, pannus, subcortical blood vessel invasion in the calcified zone, increasing the amount of isogenous groups and thickness of the calcified zone were observed. CONCLUSION: Exercise climb ladder was effective in cartilaginous tissue recovery process damaged by immobilization, in model of osteoporosis by ovariectomy in rats.


Assuntos
Cartilagem Articular/patologia , Terapia por Exercício/métodos , Imobilização/efeitos adversos , Atividade Motora/fisiologia , Osteoporose/terapia , Tíbia/patologia , Animais , Cartilagem Articular/metabolismo , Contagem de Células , Condrócitos/patologia , Modelos Animais de Doenças , Feminino , Lâmina de Crescimento/metabolismo , Osteoporose/etiologia , Osteoporose/patologia , Ovariectomia , Ratos Wistar
15.
Rev Bras Reumatol ; 55(1): 43-7, 2015.
Artigo em Português | MEDLINE | ID: mdl-25457623

RESUMO

OBJECTIVE: To verify the functionality through muscle grip strength in animals with obesity induced by monosodium glutamate (MSG) and in control animals, which suffered compression of the right median nerve, and treated with swimming with overload. METHODS: During the first five days of life, neonatal Wistar rats received subcutaneous injections of MSG. The control group received a hypertonic saline solution. Forty-eight rats were divided into six groups: G1 (control); G2 (control + injury); G3 (control + injury + swimming); G4 (obese); G5 (obese + injury); G6 (obese + injury + swimming). The animals in groups G2, G3, G5 and G6 were submitted to compression of the median nerve and G3 and G6 groups were treated, after injury, with swimming exercise with load for three weeks. The swimming exercise had a progressive duration, according to the week, of 20, 30 and 40minutes. Muscle strength was assessed using a grip strength meter preoperatively and on the 3rd, 7th, 14th and 21st days after surgery. The results were expressed and analyzed using descriptive and inferential statistics. RESULTS: When the grip strength was compared among assessments regardless of group, in the second assessment the animals exhibited lower grip strength. G1 and G4 groups had greater grip strength, compared to G2, G3, G4 and G6. CONCLUSION: The swimming exercise with overload has not been effective in promoting improvement in muscle grip strength after compression injury of the right median nerve in control and in obese-MSG rats.


Assuntos
Força da Mão , Síndromes de Compressão Nervosa/fisiopatologia , Obesidade/fisiopatologia , Natação , Animais , Nervo Mediano , Síndromes de Compressão Nervosa/complicações , Obesidade/complicações , Ratos , Ratos Wistar , Suporte de Carga
16.
Rev. dor ; 15(4): 287-289, 2014. tab
Artigo em Inglês | LILACS-Express | ID: lil-730607

RESUMO

BACKGROUND AND OBJECTIVES: Among electro agents ultrasound is one of the most common, however, there is insufficient evidence of the beneficial effects with the parameters currently used. The aim of this study was to compare the effect of continuous and pulsed ultrasound therapy on experimental hyperalgesia and edema in knees of Wistar rats. METHODS: 18 rats were divided into three groups: CG- control group; GUP - treated with pulsed ultrasound 50%; and GUC - continuous ultrasound. To accomplish the lesion, animals were manually restrained and 100μL of 5% formalin solution were injected into the right tibiofemoral space. For assessment of nociception digital Von Frey filament was used on the medial side of the joint, until clinching. Edema was evaluated with mid-lateral knee caliper. Assessments occurred in the preinjury (EV1), after 15 (EV2), 30 (EV3) and 60 (EV4) minutes of the injury. After EV2, treatment was initiated with ultrasound with 0.4W/cm2 (SATA), pulsed or continuous. RESULTS: The CG had hypernociception, with no return to baseline. GUP has returned to baseline as from EV3 and for continuous ultrasound in EV4. All three groups showed similar behavior for edema, with onset in EV2, without reduction. CONCLUSION: Therapeutic ultrasound was effective to decrease nociception, and the pulsed form showed early results, however, both forms of application had no effect on the formation and maintenance of acute edema. .


JUSTIFICATIVA E OBJETIVOS: Dentre os agentes eletrotérmicos o ultrassom é um dos mais comuns, contudo, há insuficiente evidência dos efeitos benéficos com os parâmetros correntemente utilizados. Assim, o objetivo deste estudo foi comparar o efeito do ultrassom terapêutico contínuo e pulsado sobre hiperalgesia e edema experimentais em joelhos de ratos Wistar. MÉTODOS: Foram utilizados 18 ratos, divididos em três grupos: GC - grupo controle; GUP - tratado com ultrassom pulsado 50%; e, GUC - ultrassom contínuo. Para realizar a lesão, os animais foram contidos manualmente e 100μL de solução de formalina a 5% foram injetados no espaço tíbio-femoral direito. Para avaliação da nocicepção foi utilizado o filamento de Von Frey digital, na face medial da articulação, até a retirada do membro. A avaliação do edema foi realizada com paquimetria médiolateral ao joelho. As avaliações ocorreram no momento pré-lesão (AV1), após 15 (AV2), 30 (AV3) e 60 (AV4) minutos da lesão. Após AV2, foi iniciado o tratamento com ultrassom com 0,4W/ cm2 (SATA), de forma pulsada ou contínua. RESULTADOS: Para GC, houve a presença de hipernocicepção, sem retorno aos valores basais. Para GUP houve retorno aos valores basais a partir de AV3 e para o ultrassom contínuo em AV4. Para o edema, os três grupos apresentaram comportamento semelhante, com formação em AV2, sem redução posterior. CONCLUSÃO: O ultrassom terapêutico mostrou-se eficaz para redução do quadro nociceptivo, sendo que a forma pulsada mostrou resultados precocemente ao contínuo, contudo, ambas as formas de aplicação não tiveram efeito sobre a formação e manutenção do edema agudo. .

17.
Artigo em Português | LILACS-Express | ID: lil-743699

RESUMO

Introdução: O músculo esquelético se adapta continuamente a estímulos. Objetivo: Analisar os efeitos da imobilização e remobilização sobre parâmetros morfológicos dos músculos sóleo e tibial anterior de ratos Wistar. Métodos: Dezoito animais foram imobilizados por 15 dias, divididos em três grupos de seis componentes cada: G1 – somente imobilizados; G2 – remobilizados livremente; G3 – remobilizados em meio aquático. Foram coletados os músculos sóleo e tibial anterior, direitos (imobilizados ou tratados) e esquerdos (controle). Resultados: A imobilização reduziu massa, diâmetro da fibra e comprimento do músculo sóleo, e massa muscular do tibial anterior. Em G2 e G3, houve aumento da massa e comprimento muscular do tibial anterior; e aumento do menor diâmetro da fibra do sóleo em G3. Conclusão: A imobilização afeta a morfologia dos músculos estudados, a remobilização livre e em meio aquático foram eficientes na recuperação do tibial anterior, enquanto para o sóleo apenas os exercícios aquáticos foram eficazes.


Introduction: Skeletal muscle has the ability to continuously adapt to different stimuli. Objective: To analyze the effects of immobilization and remobilization on morphology parameters of soleus and tibial anterior of Wistar rats. Methods: Eighteen animals were immobilized for 15 days and divided into three groups of six animals each: G1 – only immobilized; G2 – remobilized freely; G3 – remobilized in water. The soleus and tibial anterior muscles both rights (immobilized or treated) and left (control) were collected. Results: Immobilization reduced muscle mass, fiber diameter and length of the soleus and muscle mass of tibial anterior. In G2 and G3 was an increase in muscle mass and length of tibial anterior; and an increase in fiber diameter on soleus in G3. Conclusion: Immobilization affects soleus and tibial anterior morphology, free and aquatic remobilization were effective in tibial anterior muscle recovery, however in the soleus muscle only the aquatic exercises were efficient.

18.
ISRN Rheumatol ; 2013: 341832, 2013.
Artigo em Inglês | MEDLINE | ID: mdl-23997964

RESUMO

The effectiveness of low-level laser therapy (LLLT) in the presence of an infectious process has not been well elucidated. The aim of the study was to evaluate the effects of LLLT in an experimental model of septic arthritis. Methods. Twenty-one Wistar rats were divided as follows: control group, no bacteria; placebo group, bacteria were inoculated; Treated group, bacteria were injected and treatment with LLLTwas performed. To assess nociception, a von Frey digital analgesimeter was applied. Synovial fluid was streaked to analyze bacterial growth. The standard strain of S. aureus was inoculated in the right knee. LLLT was performed with 660 nm, 2 J/cm(2), over 10 days. After treatment, the knees were fixed and processed for morphological analysis by light microscopy. Results. It was found that nociception increases in the right knee. There was a lack of results for the seeding of the synovial fluid. The morphological analysis showed slight recovery areas in the articular cartilage and synovia; however, there was the maintenance of the inflammatory infiltrate. Conclusion. The parameters used were not effective in the nociception reduction, even with the slight tissue recovery due to the maintenance of inflammatory infiltrate, but produced no change in the natural history of resolution of the infectious process.

19.
ScientificWorldJournal ; 2013: 476890, 2013.
Artigo em Inglês | MEDLINE | ID: mdl-23935419

RESUMO

The carpal tunnel syndrome is the most common peripheral neuropathy in the upper limb, but its treatment with conservative therapies such as neural mobilization (NM) is still controversial. The aim of this study was to investigate the efficacy of the NM as treatment in a model of median nerve compression. 18 Wistar rats were subjected to compression of the median nerve in the right elbow proximal region. Were randomly divided into G1 (untreated), G2 (NM for 1 minute), and G3 (NM for 3 minutes). For treatment, the animals were anesthetized and the right forelimb received mobilization adapted to humans, on alternated days, from the 3rd to the 13th day postoperatively (PO), totaling six days of therapy. Nociception was assessed by withdrawal threshold, and after euthanasia histomorphometric analysis of the median nerve was performed. The nociceptive evaluation showed in G2 and G3 delay in return to baseline. Histomorphometric analysis showed no significant differences in the variables analyzed. It is concluded that the NM was not effective in reducing nociceptive sensation and did not alter the course of nerve regeneration.


Assuntos
Síndrome do Túnel Carpal/terapia , Modelos Animais de Doenças , Nervo Mediano/lesões , Nociceptividade , Modalidades de Fisioterapia , Animais , Ratos , Ratos Wistar
20.
Rev. Kairós ; 15(3): 67-80, set.2012.
Artigo em Português | LILACS | ID: lil-766879

RESUMO

Objetivou-se analisar a satisfação com a imagem corporal de 24 idosas ativas, e compreender a visão destas sobre a relação do exercício físico com sua imagem corporal. Todas responderam à escala proposta por Stunkard, Sorenson e Schlusinger (1983) e a uma entrevista semiestruturada. Estavam insatisfeitas com sua imagem corporal, 87,50%. A partir da leitura e análise dos discursos, formaram-se 2 categorias principais e 4 subcategorias. Assim, a maioria das idosas está insatisfeita com sua imagem corporal; porém, são reconhecidos os benefícios proporcionados pelo exercício físico...


The aim of this paper was to analyze the body image satisfaction with 24 active elderly women, and to understand the view of these people about the connection between physical exercise and their body image. All of them answered to the scale proposed by Stunkard, Sorenson and Schlusinger, 1983 and to a semi-structured interview. 87.50% of the women were unsatisfied about the body image. From the reading and analysis of the speeches, it was formed two major categories and four subcategories. Thus, most of the elderly women are unsatisfied about their body image, but the proportionate benefits by the exercises are recognized...


Assuntos
Humanos , Feminino , Idoso , Idoso , Imagem Corporal , Exercício
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