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1.
Acta Histochem ; 122(5): 151568, 2020 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-32622429

RESUMO

Structural changes in parotid gland (PG) were previously reported following ablation of thyroid gland. However, the functional alterations (especially for proteins) have not been elucidated yet. Herein, we investigated the effect of rat thyroidectomy on PG structure and protein content and studied the ability of thyroxin-supplementation to alleviate the associated structural and functional changes. Male young adult 4-month old albino rats (n = 48) were allocated equally into 4 groups (control, sham-operated, thyroidectomized, and thyroxin-supplemented). PGs were examined histologically, and their proteins expression and localization were analyzed using western blot (WB) and immunohistochemistry (IHC), respectively at 3 w and 5 w post-surgery. Functionally, PGs of thyroidectomized rats formed a newly expressed 300 KDa protein, which was confirmed to be thyroglobulin (TG) by WB and IHC, with higher expression at 5 w. TG was localized in the interstitium, within capillaries, in the cytoplasm of the intralobular ductal cells, in the secretory products within the ductal lumen, and in the cytoplasm of individual small cells at the periphery of the acini. This functional change accompanied by structural changes in PGs (presence of dark and light acinar cells, TG-like colloid material, and high periductal vasculature). Noteworthy, PG of the thyroxin-supplemented depicted vanishment of TG. From these data, it could be concluded that thyroidectomy could alter the morphology and function of the parotid that induce a thyroid-like reprogramming of the parotid to secrete TG and thyroxin supplementation could alleviate this effect.


Assuntos
Glândula Parótida/cirurgia , Glândulas Salivares/cirurgia , Tireoglobulina/metabolismo , Glândula Tireoide/metabolismo , Animais , Citoplasma/metabolismo , Imuno-Histoquímica/métodos , Glândula Parótida/metabolismo , Ratos Sprague-Dawley , Glândulas Salivares/metabolismo , Glândula Submandibular/metabolismo , Glândula Submandibular/cirurgia , Tireoidectomia/efeitos adversos
2.
J Conserv Dent ; 22(1): 17-22, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-30820077

RESUMO

Background: This study investigated cytotoxic probability, osteogenic potential, and antibacterial efficacy of two pulp-capping hydraulic calcium-silicate cements. Materials and Methods: For osteogenic potential and cytotoxicity evaluation, mesenchymal stem cells (MSCs) and materials disc-shaped specimens were used. Increase or decrease in a number of proliferating MSCs was calculated after three intervals. Alkaline phosphatase (ALP) levels in osteogenic media were normalized to the total protein content of cells and measured spectrophotometrically. Antibacterial efficiency through growth curves of Streptococcus mutans in direct contact with tested materials. Results: Biodentine showed the highest number of proliferating MSCs (278000.41 ± 4000.06, after 72 h) and the highest concentration of ALP after 12 days (209.26 ± 7.17 µU/µg protein). It showed the lowest slope (0.003 ± 0.0005) of S. mutans strains growth curves after 18 h. Conclusion: Biodentine proved a highly significant osteogenic ability and gave a significant reduction of S. mutans growth.

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