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1.
Molecules ; 27(7)2022 Mar 24.
Artigo em Inglês | MEDLINE | ID: mdl-35408484

RESUMO

Cell culturing methods in its classical 2D approach have limitations associated with altered cell morphology, gene expression patterns, migration, cell cycle and proliferation. Moreover, high throughput drug screening is mainly performed on 2D cell cultures which are physiologically far from proper cell functions resulting in inadequate hit-compounds which subsequently fail. A shift to 3D culturing protocols could solve issues with altered cell biochemistry and signaling which would lead to a proper recapitulation of physiological conditions in test systems. Here, we examined porous ultra-high molecular weight polyethylene (UHMWPE) as an inexpensive and robust material with varying pore sizes for cell culturing. We tested and developed culturing protocols for immortalized human neuroblastoma and primary mice hippocampal cells which resulted in high rate of cell penetration within one week of cultivation. UHMWPE was additionally functionalized with gelatin, poly-L-lysine, BSA and chitosan, resulting in increased cell penetrations of the material. We have also successfully traced GFP-tagged cells which were grown on a UHMWPE sample after one week from implantation into mice brain. Our findings highlight the importance of UHMWPE use as a 3D matrix and show new possibilities arising from the use of cheap and chemically homogeneous material for studying various types of cell-surface interactions further improving cell adhesion, viability and biocompatibility.


Assuntos
Técnicas de Cultura de Células , Polietilenos , Animais , Técnicas de Cultura de Células/métodos , Células Cultivadas , Camundongos , Peso Molecular , Polietileno/química , Polietilenos/química , Porosidade
2.
Ann N Y Acad Sci ; 1090: 177-81, 2006 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-17384260

RESUMO

Like many other transcription factors, the tumor suppressor protein p53 is bound to the nuclear matrix (NM). To study the interaction of p53 with the NM in more detail, we used alkaline and acidic extractions of NM proteins. It was found that there are two forms of p53, alkali- and acid-soluble, in NM of HEK293 cells and only one alkali-soluble form in NM of actinomycin D-treated MCF-7 cells. We suggest that distinct forms of p53 differ either in interactions with NM proteins or in their charges.


Assuntos
Matriz Nuclear/metabolismo , Linhagem Celular , Humanos , Ligação Proteica , Proteína Supressora de Tumor p53/metabolismo
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