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1.
Sci Rep ; 11(1): 10654, 2021 05 20.
Artigo em Inglês | MEDLINE | ID: mdl-34017038

RESUMO

The purpose of this study was to determine if a methanolic extract of the Pulsatilla patens (L.) Mill. can inhibit the progression of cancer through the modulation of cancer-related metabolic signaling pathways. We analyzed a panel of 13 inducible luciferase reporter gene vectors which expression is driven by enhancer elements that bind to specific transcription factors for the evaluation of the activity of cancer signaling pathways. The root extract of P. patens exhibited strong inhibition of several signaling pathways in HeLa cells, a cervical cancer cell line, and was found to be the most potent in inhibiting the activation of Stat3, Smad, AP-1, NF-κB, MYC, Ets, Wnt and Hdghog, at a concentration of 40 µg/mL. The methanolic extracts of P. patens enhanced apoptotic death, deregulated cellular proliferation, differentiation, and progression towards the neoplastic phenotype by altering key signaling molecules required for cell cycle progression. This is the first study to report the influence of Pulsatilla species on cancer signaling pathways. Further, our detailed phytochemical analysis of the methanolic extracts of the P. patens allowed to deduce that compounds, which strongly suppressed the growth and proliferation of HeLa cancer cells were mainly triterpenoid saponins accompanied by phenolic acids.


Assuntos
Neoplasias/metabolismo , Extratos Vegetais/farmacologia , Pulsatilla/química , Transdução de Sinais , Morte Celular/efeitos dos fármacos , Ácidos Cumáricos/farmacologia , Genes Reporter , Células HeLa , Humanos , Limite de Detecção , Luciferases/metabolismo , Metanol , Proteínas de Neoplasias/metabolismo , Neoplasias/patologia , Raízes de Plantas/química , Reprodutibilidade dos Testes , Saponinas/química , Saponinas/farmacologia , Transdução de Sinais/efeitos dos fármacos , Triterpenos/química , Triterpenos/farmacologia
2.
Am J Case Rep ; 21: e926299, 2020 Jul 30.
Artigo em Inglês | MEDLINE | ID: mdl-32728016

RESUMO

BACKGROUND Traumatic injury of the thoracic aorta is proving to be not only the most lethal of traumatic injuries, but also the most urgent reason for vascular intervention among all trauma patients. Endovascular aortic repair is used increasingly often to treat traumatic injuries. We report a case of endovascular treatment and its use as a delayed correction (two-stage treatment) for a traumatic aortic isthmus rupture. CASE REPORT A 20-year-old Asian male was admitted to our department after a car accident presenting symptoms of ischemic shock. Among multiple injuries, a traumatic descending aorta rupture was diagnosed. The patient was referred directly to the operating room for a thoracic endovascular aortic repair (TEVAR). The patient's other trauma-related injuries required additional interventions in the following days. Thirty days after the emergent TEVAR operation, the patient required reintervention due to a major type-I endoleak. Computed tomography angiography revealed a failed stentgraft deployment. We removed the mismatched endovascular equipment and deployed an appropriately sized stentgraft during a hybrid procedure, excluding the ruptured aortic wall altogether. CONCLUSIONS Endovascular treatment of both children and small-framed adults remains a challenge for operating teams. First, no dedicated equipment can be found on the market. Second, measuring and fitting endovascular equipment constitutes a sore point in treatment, so in emergency situations, only off-the-shelf tools are accessible. We assert that, in such cases, the primary procedure should be understood as a lifesaving intervention, awaiting a final and long-lasting solution.


Assuntos
Aorta Torácica/lesões , Procedimentos Endovasculares , Stents Metálicos Autoexpansíveis , Acidentes de Trânsito , Aorta Torácica/diagnóstico por imagem , Aorta Torácica/cirurgia , Angiografia por Tomografia Computadorizada , Humanos , Masculino , Ajuste de Prótese , Ruptura/cirurgia , Adulto Jovem
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