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J Med Virol ; 93(4): 2439-2445, 2021 04.
Artigo em Inglês | MEDLINE | ID: mdl-33368332


Diagnosis of severe acute respiratory syndrome coronavirus 2 (SARS-COV-2) cases is based on the count of real-time reverse transcription-plymerase chain reaction (RT-PCR) positive people. Viral load by real-time RT-PCR has been suggested as a biomarker of the SARS-CoV-2 infection. However, the association of viral load and severity of the disease is not yet resolved. Nasopharyngeal samples from 458 patients were tested by RT-PCR for SARS-CoV-2 diagnosis. Relative quantitation was made by the comparative threshold cycle (ΔΔCt ) formula between ORF1ab viral and RNase P housekeeping genes. Absolute viral load was calculate using a reference positive control. Most prevalent clinical signs were cough (75.8%), myalgia (66.7%), and fever (48.5%). Hypertension (18.2%), neurological diseases (15.1%), and asthma and hypothyroidism (12.1%) were most frequent comorbidities. Fever, either as an exclusive symptom or combined with others, was associated with high viral loads ( 2 - ∆ ∆ C t range, 35.65-155.16; 4.25-4.89 log10 RNA copies/test]). During the first week after onset of symptoms in mild patients up to 60 years-old was detected the peak of viral load. Children under 10 years old have a high viral load (313.84; 2.50) in the first 2 days postinfection with a sharp decline thereafter. Cases between 10 and 49 years old mostly showed low and moderate viral load during the first 2 days postinfection (range, 0.03 to 17.24; -1.50 to 1.24). Patients over 60 years old have high viral load up to the second week after the onset of symptoms (range, 25.32-155.42; 1.40-2.19), indicating the longer presence of the virus in them. These findings suggest the viral load in nasopharyngeal swabs would help to monitor the SARS-CoV-2 infection in mild coronavirus disease 2019 cases.

Front Public Health ; 8: 562615, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-33072699


The pandemic caused by the new severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is a worldwide public health concern. First confined in China and then disseminated widely across Europe and America, SARS-CoV-2 has impacted and moved the scientific community around the world to working in a fast and coordinated way to collect all possible information about this virus and generate new strategies and protocols to try to stop the infection. During March 2020, more than 16,000 full viral genomes have been shared in public databases that allow the construction of genetic landscapes for tracking and monitoring the viral advances over time and study the genomic variations present in geographic regions. In this work, we present the occurrence of genetic variants and lineages of SARS-CoV-2 in Chile during March to April 2020. Complete genome analysis of 141 viral samples from different regions of Chile revealed a predominance of variant D614G like in Europe and the USA and the major presence of lineage B.1. These findings could help take control measures due to the similarity of the viral variants present in Chile, compared with other countries, and monitor the dynamic change of virus variants in the country.

J Med Virol ; 92(9): 1562-1566, 2020 09.
Artigo em Inglês | MEDLINE | ID: mdl-32222995


The current pandemic caused by the new coronavirus is a worldwide public health concern. To aboard this emergency, and like never before, scientific groups around the world have been working in a fast and coordinated way to get the maximum of information about this virus when it has been almost 3 months since the first cases were detected in Wuhan province in China. The complete genome sequences of around 450 isolates are available, and studies about similarities and differences among them and with the close related viruses that caused similar epidemics in this century. In this work, we studied the complete genome of the first four cases of the new coronavirus disease in Chile, from patients who traveled to Europe and Southeast Asia. Our findings reveal at least two different viral variants entries to Chilean territory, coming from Europe and Asia. We also sub-classified the isolates into variants according to punctual mutations in the genome. Our work contributes to global information about transmission dynamics and the importance to take control measures to stop the spread of the infection.

Open Biol ; 7(11)2017 11.
Artigo em Inglês | MEDLINE | ID: mdl-29118270


The hallmark of high-risk human papillomavirus (HR-HPV)-related carcinogenesis is E6 and E7 oncogene overexpression. The aim of this work was to characterize epithelial oral and cervical cancer cells that express HR-HPV E6 and E7 oncoproteins. Transcriptomic assay using DNA microarrays revealed that PIR gene expression was detected in oral cells in an HR-HPV E6/E7-dependent manner. In addition, PIR was overexpressed in HPV-positive SiHa and Ca Ski cells, whereas it was undetectable in HPV-negative C33A cells. The PIR expression was dependent on functional HR-HPV E6 and E7 oncoproteins even though the E7 oncoprotein had higher activity to induce PIR overexpression in comparison with E6. In addition, using an siRNA for PIR silencing in oral cells ectopically expressing HR-HPV E6/E7, there was a significant increase in E-cadherin transcripts and a decrease in Vimentin, Slug, Zeb and Snail transcripts, suggesting that HR-HPV-induced PIR overexpression is involved in epithelial-mesenchymal transition. Furthermore, migration of PIR-silenced cells was significantly decreased. Finally, using inhibitors of some specific pathways, it was found that EGFR/ERK and PI3 K/AKT signalling pathways are important for E7-mediated PIR overexpression. It can be concluded that PIR gene expression is highly dependent on the expression of HR-HPV oncoproteins and is important for EMT regulation.

Proteínas de Transporte/genética , Proteínas Nucleares/genética , Proteínas Oncogênicas Virais/metabolismo , Papillomaviridae/metabolismo , Proteínas de Transporte/metabolismo , Linhagem Celular Tumoral , Colo do Útero/citologia , Colo do Útero/virologia , Dioxigenases , Receptores ErbB/metabolismo , Feminino , Regulação Neoplásica da Expressão Gênica , Humanos , Proteína Quinase 3 Ativada por Mitógeno/metabolismo , Mucosa Bucal/citologia , Mucosa Bucal/virologia , Proteínas Nucleares/metabolismo , Fosfatidilinositol 3-Quinases/metabolismo , Proteínas Proto-Oncogênicas c-akt/metabolismo , Transdução de Sinais , Fatores de Transcrição da Família Snail/genética , Fatores de Transcrição da Família Snail/metabolismo , Regulação para Cima , Vimentina/genética , Vimentina/metabolismo
F1000Res ; 3: 305, 2014.
Artigo em Inglês | MEDLINE | ID: mdl-25671088


Odorant-binding proteins (OBPs), also named pheromone-binding proteins when the odorant is a pheromone, are essential for insect olfaction. They solubilize odorants that reach the port of entry of the olfactory system, the pore tubules in antennae and other olfactory appendages. Then, OBPs transport these hydrophobic compounds through an aqueous sensillar lymph to receptors embedded on dendritic membranes of olfactory receptor neurons. Structures of OBPs from mosquito species have shed new light on the mechanism of transport, although there is considerable debate on how they deliver odorant to receptors. An OBP from the southern house mosquito, Culex quinquefasciatus, binds the hydrophobic moiety of a mosquito oviposition pheromone (MOP) on the edge of its binding cavity. Likewise, it has been demonstrated that the orthologous protein from the malaria mosquito binds the insect repellent DEET on a similar edge of its binding pocket. A high school research project was aimed at testing whether the orthologous protein from the yellow fever mosquito, AaegOBP1, binds DEET and other insect repellents, and MOP was used as a positive control. Binding assays using the fluorescence reporter N-phenyl-1-naphtylamine (NPN) were inconclusive. However, titration of NPN fluorescence emission in AaegOBP1 solution with MOP led to unexpected and intriguing results. Quenching was observed in the initial phase of titration, but addition of higher doses of MOP led to a stepwise increase in fluorescence emission coupled with a blue shift, which can be explained at least in part by formation of MOP micelles to house stray NPN molecules.

Acta méd. (Porto Alegre) ; 33(1): [6], 21 dez. 2012.
Artigo em Português | LILACS | ID: biblio-879477


Análise dos mecanismos fisiopatológicos, manifestações clínicas, diagnóstico e tratamento de oclusão arterial aguda.

Analysis of the pathophysiological mechanisms, clinical manifestations, diagnosis and treatment of acute arterial occlusion.

Procedimentos Cirúrgicos Vasculares
Acta méd. (Porto Alegre) ; 33(1)21 dez. 2012.
Artigo em Português | LILACS | ID: biblio-882329


Esse artigo é uma revisão bibliográfica acerca do diagnóstico e do tratamento de taquiarritmias em situações de urgência e emergência, com foco nas indicações de cardioversão e desfibrilação. Ele aborda a utilização, técnica e complicações da terapia elétrica, bem como suas principais indicações: parada cardiorrespiratória em fibrilação e taquicardia ventricular, fibrilação e flutter atrial e taquicardia supraventricular com reentrada.

This article is a bibliographic review about the diagnosis and treatment of tachyarrythmias in urgency and emergency scenario, focusing on the indications of cardioversion and defibrillation. It approaches the utilization, technique and complications of electric therapy, as well as its main indications: cardiac arrest in ventricular fibrillation and tachycardia, atrial fibrillation and flutter and reentrancy supraventricular tachycardia.

Cardioversão Elétrica , Taquicardia/terapia , Emergências , Taquicardia/diagnóstico
Rev. chil. obstet. ginecol ; 54(3): 115-23, 1989. tab, ilus
Artigo em Espanhol | LILACS | ID: lil-82606


Se presentan los resultados de 110 ciclos menstruales de 87 pacientes que fueron estudiadas con seguimiento ecográfico de la ovulación, determinación inmunológica de LH, curva de temperatura basal y estudio seriado del score cervical, desde el día -6 al día 0; el seguimiento ecográfico comprendió la determinación del diámetro folicular, el grosor endometrial y la presencia de líquido libre en el fondo del saco de Douglas. Se consideran sólo aquellas pacientes en que la última observación del folículo fue hecha hasta 24 horas de su desaparición, siendo éste el hecho que indica la ovulación, relacionándose las demás veriables con ello. Estas pacientes presentan ciclos espontáneos (47,3%), inducidos con epimestrol (28,2%) o clomifeno (24,5%) y resultaron embarazadas o no en este ciclo, 34,6%; 25,8% y 37,0% respectivamente. Fueron 36 casos con embarazo (32,7%). Las tres curvas de diámetro folicular para ciclos con embarazo resultaron notablemente similares, siendo el tamaño folicular preovulatorio de 21,5 mm. para ciclos espontáneos, 18,9 mm para ciclos inducidos con epimetrol y 20,7 mm para ciclos inducidos con clomifeno. El rango en los ciclos concepcionales fue de 15 a 27 mm, 15 a 22 mm y 14 a 27 mm, respectivamente. En 5 ciclos con embarazo (13,9%) no se observó líquido libre en el Douglas. La mayoría de los LH (+) se agrupan en los días -2 y -1 (21% y 50% respectivamente). Hubo una paciente que se embarazó y 2 que no se embarazaron que tuvieron LH iterativamente negativa. En el 50% de las pacientes que se embarazaron el Nadir térmico fue el día -1. En el grupo espontáneo concepcional no se observaron malos scores cervicales. El período de buen score es más largo en los grupos concepcionales espontáneos y con epimestrol. En el grupo de ciclos con embarazo no se observó disociación entre el día del mejor score y la ovulación, hecho que sí se observa en los ciclos no concepcionales

Gravidez , Humanos , Feminino , Ovulação , Indução da Ovulação , Ultrassonografia , Clomifeno , Epimestrol , Fase Folicular , Hormônio Luteinizante/urina , Detecção da Ovulação
In. Sociedad Paraguaya de Fertilidad. Esterilidad conyugal. s.l, EFACIM, mayo 1987. p.114-36, ilus, tab.
Monografia em Espanhol | LILACS | ID: lil-69322