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2.
Artigo em Inglês | MEDLINE | ID: mdl-32384634

RESUMO

A growing awareness has recently emerged on the health benefits of exposure to daylight and views. Daylight exposure is linked to circadian rhythm regulation, which can have significant impacts on sleep quality and cognitive function. Views of nature have also been shown to impact emotional affect and performance. This study explores the impact of optimized daylight and views on the sleep and cognitive performance of office workers. Thirty knowledge workers spent one week working in each of two office environments with identical layouts, furnishings, and orientations; however, one was outfitted with electrochromic glass and the other with traditional blinds, producing lighting conditions of 40.6 and 316 equivalent melanopic lux, respectively. Participants in the optimized daylight and views condition slept 37 min longer as measured by wrist-worn actigraphs and scored 42% higher on cognitive simulations designed to test their higher order decision-making performance. Both sleep and cognitive function were impacted after one day in the space, yet the impacts became more significant over the course of the week. The positive effect of optimized daylight and views on cognitive function was comparable for almost all participants, while increases in sleep duration were significantly greater for those with the lowest baseline sleep duration. This study stresses the significance of designing with daylight in order to optimize the sleep quality and performance of office workers.

3.
Clin Biomech (Bristol, Avon) ; 75: 105005, 2020 May.
Artigo em Inglês | MEDLINE | ID: mdl-32353689

RESUMO

BACKGROUND: Although partial meniscectomy is a common treatment for the tears in the avascular region of the meniscus, mechanical alterations following meniscectomy are known to initiate mechanically-induced osteoarthritis. We aimed to measure the articular cartilage contact pressure distributions in the knees with surgically repaired and partially resected menisci in the avascular region. METHODS: A pneumatic loading device was developed to apply a 1000 N compressive load on the cadaveric porcine knee samples at the flexion angles of 20, 35, 50, and 65°. We simulated longitudinal meniscal tears in the avascular inner 1/3 portion and the well-vascularized middle 1/3 portion of the meniscus. Articular cartilage contact pressures for the knees with intact, torn, repaired, and resected menisci were compared. FINDINGS: For the tears in well-vascularized regions, meniscal repairs restored articular cartilage contact pressures to the levels in intact joints. However, partial meniscectomy significantly increases the maximum contact pressures and the average contact pressures in highly compressed areas. However, partial meniscectomy in the avascular region did not alter the maximum articular cartilage contact pressures and the average contact pressures in highly compressed areas. Stabilities in knee samples were not significantly altered following partial meniscectomy in both inner and middle regions. INTERPRETATION: Although repair surgeries are beneficial for the tears in well-vascularized areas because the articular cartilage contact mechanics are reconstructed, partial meniscectomy may be a viable alternative treatment for the tears in avascular regions without introducing significant mechanical alterations.

4.
J Chemother ; : 1-9, 2020 May 20.
Artigo em Inglês | MEDLINE | ID: mdl-32431230

RESUMO

Whether plasma MTX concentrations and MTHFR C677T and A1298C polymorphisms could be used as a predictor of occurrence of MTX-related toxicities in Korean paediatric patients with acute lymphoblastic leukaemia (ALL) were assessed. HD-MTX related toxicities, MTHFR polymorphisms and MTX plasma concentrations following 337 HD-MTX cycles to 117 children with ALL on maintenance therapy were analyzed. A significantly higher frequency of hyperbilirubinemia (P = 0.0443) and renal toxicity (P = 0.0107) were associated with high MTX concentrations by Fisher's exact test. Moreover, high MTX concentrations at 24 h, 48, and 72 h were significantly associated with increased frequency of vomiting (P < 0.05) and hyperbilirubinemia (P < 0.05) by Mann-Whitney U test. There was a significantly higher frequency of mucositis in patients with the MTHFR 677 TT genotype (P = 0.0273) and a significantly higher frequency of MTX dose reduction in patients with the 677 TT genotype (P = 0.0217), compared to the CC/CT genotype. Independently, plasma MTX concentrations and MTHFR C677T genotype could be useful markers for tailoring MTX dosing and monitoring adverse effects in childhood ALL HD-MTX therapy in Korean patients.

5.
J Acoust Soc Am ; 147(3): 1418, 2020 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-32237802

RESUMO

The goal of this study is to determine potential intelligibility benefits from Lombard speech for cochlear implant (CI) listeners in speech-in-noise conditions. "Lombard effect" (LE) is the natural response of adjusting speech production via auditory feedback due to noise exposure within acoustic environments. To evaluate intelligibility performance of natural and artificially induced Lombard speech, a corpus was generated to create natural LE from large crowd noise (LCN) exposure at 70, 80, and 90 dB sound pressure level (SPL). Clean speech was mixed with 15 and 10 dB SNR LCN and presented to five CI users. First, speech intelligibility was analyzed as a function of increasing LE and decreasing SNR. Results indicate significant improvements (p < 0.05) with Lombard speech intelligibility in noise conditions for 80 and 90 dB SPL. Next, an offline perturbation strategy was formulated to modify/perturb neutral speech so as to mimic LE through amplification of highly intelligible segments, uniform time stretching, and spectral mismatch filtering. This process effectively introduces aspects of LE into the neutral speech, with the hypothesis that this would benefit intelligibility for CI users. Significant (p < 0.01) intelligibility improvements of 13% and 16% percentage points were observed for 15 and 10 dB SNR conditions respectively for CI users. The results indicate how LE and LE-inspired acoustic and frequency-based modifications can be leveraged within signal processing to improve intelligibility of speech for CI users.

6.
Nanoscale ; 2020 Apr 02.
Artigo em Inglês | MEDLINE | ID: mdl-32239078

RESUMO

Control of the chemical and physical properties of nanoscale colloids and their nanoassemblies remains a challenging issue for enhancing the performance and functionalities of nanodevices. In this study, we report a post-synthesis etching method to tailor the porosity of the Fe3O4 shells coating on Ag NPs, establishing a facile but effective approach to regulate the chemical and optical properties of the colloids and their assembled structures. As the shell porosity increases, the NPs are transformed, producing enhanced catalytic activity and the surface-enhanced Raman spectroscopy (SERS) effect, which results from enhanced chemical diffusion into the Ag core. Magnetoplasmonic (MagPlas) one- (1D) and two- (2D) dimensional arrays fabricated using these porosity-controllable NPs exhibit intriguing plasmon properties that are strongly affected by the porosity of the particle shell. Furthermore, the bright coloration of the 2D arrays is tuned by changing the shell porosity or introducing an additional metallic layer. Such 1D and 2D porous MagPlas metastructures possessing Fe3O4 shells with tunable porosities are a fulcrum for developing recyclable catalysts and tunable optical filters with optimized activity, selectivity, and sensitivity, as well as color displays and sensing platforms.

7.
Chin J Nat Med ; 18(2): 114-122, 2020 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-32172947

RESUMO

Glioblastoma is the deadliest neoplasm with the worst 5-year survival rate among all human cancers. Autophagy promotes autophagic cell death or blocks the induction of apoptosis in eukaryotic cells. Here, we investigated whether varying levels of autophagic flux in glioblastoma lead to different efficacies of curcumin treatment using U87MG and A172 human glioblastoma cells. The number of LC3 puncta, the number of cells with LC3 puncta and the level of LC3 II, Atg5 and Atg7 protein were higher in U87MG cells compared with A172 cells. When the cells were incubated with curcumin for 24 or 48 h, the percentage of cell death was higher in A172 cells compared with U87MG cells. Although the level of LC3 was lower, that of curcumin-induced LC3 was higher, in A172 cells than in U87MG cells. The relative increases in cell death and LC3-mediated autophagy were greater under serum starvation in A172 cells compared with U87MG cells. Curcumin-induced A172 cell death was reduced by serum starvation. When both types of cells were transfected with LC3-GFP, the percentage of cell death was higher in A172 cells than that in U87MG cells. Taken together, the data demonstrate that curcumin-mediated tumor cell death is regulated by the basal level of autophagic flux in different glioblastoma cells. This suggests that prior to the use of various curcumin therapeutics, the level of basal or induced autophagic flux should be carefully examined in tumor cells for the best efficacy.

8.
Environ Pollut ; 259: 113907, 2020 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-32023790

RESUMO

B cells contribute to produce inflammatory cytokines and antibodies, to present autoantigens, and to interact with T cells, which lead to body defense and disease control. Nuclear factor (erythroid-derived 2)-like 2(Nrf2) is responsible for gene expression of antioxidant enzymes to protect cells from oxidative stress by reactive oxygen species(ROS) production. Bisphenol A(BPA) may not be safe due to the effect on body's physiological functions. The chemicals that substitute for BPA may still have similar effects in the body. Tritan™ copolyester is a novel plastic form using BPA substitutes, 1,4-cyclohexanedimethanol(CHDM), dimethyl terephthalate(DMT), and 2,2,4,4-tetramethyl-1,3-cyclobutanediol(TMCD). Isosorbide(ISO) was also used as a substitute for TMCD and DMT. Here, we investigated whether B cell viability is influenced by BPA and its substitutes via Nrf2 induction using WiL2-NS human B lymphoblast cells. When cytotoxicity was measured by using assays with MTT, CellTiter-Glo, trypan blue and propidium iodide, cytotoxicity by BPA was higher than that by substitutes. BPA and its substitutes showed significant cytotoxicity and ROS production, which were attenuated by the treatment with N-acetylcysteine(NAC), a ROS scavenger. In addition, BPA treatment enhanced gene expression of antioxidant enzymes, heme oxygenase(HO)-1, catalase, superoxide dismutase(SOD) 1 and 2. As H2O2 treatment induced cell death and Nrf2 amount in WiL2-NS cells, BPA treatment increased Nrf2. Cell death by H2O2 was increased in doxycycline-inducible Nrf2-knockdown(KD) cells. In Cytotoxicity by the treatment with BPA or its substitutes was also enhanced in Nrf2-KD cells but that was reduced by Nrf2 overexpression compared to control cells. Taken together, these results implicate that B cell cytotoxicity by substitutes should be lower than BPA and Nrf2 can prevent B cells from BPA- or BPA substitutes-induced cytotoxicity via ROS production. Data suggest that the comprehensive studies or evaluation could be necessary to replace BPA in manufacture by other substitutes.


Assuntos
Linfócitos B , Compostos Benzidrílicos , Regulação da Expressão Gênica , Peróxido de Hidrogênio , Fator 2 Relacionado a NF-E2 , Fenóis , Linfócitos B/citologia , Linfócitos B/efeitos dos fármacos , Compostos Benzidrílicos/química , Compostos Benzidrílicos/toxicidade , Sobrevivência Celular/efeitos dos fármacos , Regulação da Expressão Gênica/efeitos dos fármacos , Humanos , Fator 2 Relacionado a NF-E2/genética , Estresse Oxidativo/efeitos dos fármacos , Oxirredutases/genética , Fenóis/química , Fenóis/toxicidade , Espécies Reativas de Oxigênio
9.
J Nanosci Nanotechnol ; 20(7): 4423-4427, 2020 Jul 01.
Artigo em Inglês | MEDLINE | ID: mdl-31968488

RESUMO

In this study, we have prepared a composite adsorbent with highly dispersed Mn and Ag nanocatalyst on the surface of activated carbon (AC) by applying the Radio-Frequency (RF) thermal plasma technique for the efficient removal of VOCs. The ACs before and after metal impregnation with RF plasma treatment were characterized by SEM, TEM, EDS, and nitrogen adsorption analysis. Adsorption behaviors of toluene, acetaldehyde, and formaldehyde on ACs before and after modification were also investigated in fixed-bed systems. The experimental adsorption results for VOCs on parent ACs and metal impregnated ACs (Mn-AC and Mn/Ag-AC) were well explained by modified continuous sigmoidal (MCS) model.

10.
Neural Netw ; 119: 139-150, 2019 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-31425854

RESUMO

Learning document representation is important in applying machine learning algorithms for sentiment analysis. Distributed representation learning models of words and documents, one of neural language models, have overcome some limits of vector space models such as bag-of-words model and have been utilized successively in many natural language processing tasks including sentiment analysis. However, because such models learn the embeddings only with a context-based objective, it is hard for embeddings to reflect the sentiment of texts. In this research, we address this problem by introducing a semi-supervised sentiment-discriminative objective using partial sentiment information of documents. Our method not only reflects the partial sentiment information, but also preserves local structures induced from original distributed representation learning objectives by considering only sentiment relationships between neighboring documents. Using real-world datasets, the proposed method has been validated by sentiment visualization and classification tasks. The visualization results of Amazon review datasets demonstrate the enhancement of the sentiment class separation when document representations of our proposed method are compared to other methods. Sentiment prediction from our representations also appears to be consistently superior to other representations in both Amazon and Yelp datasets. This work can be extended to develop effective document embeddings applied to other discriminative tasks.


Assuntos
Aprendizado de Máquina , Processamento de Linguagem Natural , Algoritmos , Humanos , Idioma
11.
Sci Rep ; 9(1): 12237, 2019 Aug 22.
Artigo em Inglês | MEDLINE | ID: mdl-31439862

RESUMO

As di(2-ethylhexyl) phthalate (DEHP), one of phthalates, is classified as probable human carcinogens in EPA, acetyltriethyl citrate(ATEC), one of aliphatic esters, could be applied to DEHP substitute. ATEC is used as plasticizers in cosmetics and nail products. Here, we studied whether ATEC might have genotoxic potential and induce glucose tolerance as compared to DEHP. Genotoxicity was determined by Ames test with histidine-requiring Salmonella typhimurium (TA98, TA100, TA1535 and TA1537) and tryptophan-requiring Escherichia coli (WP2uvrA(pKM101)) strains, chromosomal aberration assay with Chinese hamster lung(CHL/IU) cells, and micronucleus test with bone marrow cells of CD-1 mice. The number of revertants was not significantly changed in Ames test. The frequency of cells with chromosome aberrations was less than 5% in ATEC- or DEHP-treated cells for 6 or 24 h. In addition, no statistically significant increase was observed for the incidence of micronucleated polychromatic erythrocytes (MNPCE) in polychromatic erythrocytes (PCE) and for the ratio of PCE among total erythrocytes at 24 or 48 h after the treatment of mice with ATEC or DEHP. In the meanwhile, blood glucose level (BGL) was increased by the treatment of mice with DEHP or ATEC for 5 consecutive days. Additional 7 days later, BGL by DEHP was recovered to normal level, but not that by ATEC. Then, taken together, our results suggest that ATEC could disrupt glucose metabolism under our experimental conditions. Therefore, although DEHP and ATEC may not be genotoxic, our data should be helpful for persons with the problem in glucose metabolism to choose products containing DEHP or ATEC.

12.
PLoS One ; 14(7): e0218046, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31276506

RESUMO

OBJECTIVE: The current paradigm in the treatment of patients with non-invasive follicular thyroid neoplasm with papillary-like nuclear features (NIFTP) is a diagnostic lobectomy rather than complete thyroidectomy and postoperative radioiodine treatment. Consequently, preoperative diagnosis of NIFTP is considered to be important. METHODS: We performed the comprehensive analysis for diagnosis of preoperative 20 NIFTPs in comparison with 41 invasive encapsulated follicular papillary thyroid carcinomas (I-EFVPTCs) using the Korean Thyroid Imaging Reporting and Data System (K-TIRADS), Bethesda System for Reporting Thyroid Cytopathology (TBSRTC), and molecular analysis for BRAF and RAS mutations. RESULTS: K-TIRADS 3 was identified as the most common sonographic diagnosis in both NIFTP and I-EFVPTC. Unlike I-EFVPTC, K-TIRADS 5 was not identified in NIFTP. AUS/FLUS was the most common cytopathological diagnosis and none of the cases were classified as malignant category in both groups, although the difference in distribution was not significant between the groups. BRAF mutation was not found in NIFTP but was present in 9.8% of cases in I-EFVPTC. The frequency of RAS mutation in I-EFVPTCs was twice as high as that of NIFTP. Wild-type BRAF and RAS in NIFTP was significantly higher than I-EFVPTC. CONCLUSION: The existence of overlapping features between the groups was evident, hence conclusive distinction between radiology, cytology and molecular analysis could not be achieved. Apparently, the diagnosis of NIFTP based on comprehensive analysis was not confirmable but could perceive or at least favor the diagnosis of NIFTP.


Assuntos
Carcinoma Papilar, Variante Folicular , Mutação , Cuidados Pré-Operatórios , Proteínas Proto-Oncogênicas B-raf/genética , Neoplasias da Glândula Tireoide , Proteínas ras/genética , Adolescente , Adulto , Idoso , Carcinoma Papilar, Variante Folicular/diagnóstico por imagem , Carcinoma Papilar, Variante Folicular/genética , Carcinoma Papilar, Variante Folicular/patologia , Carcinoma Papilar, Variante Folicular/cirurgia , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Neoplasias da Glândula Tireoide/diagnóstico por imagem , Neoplasias da Glândula Tireoide/genética , Neoplasias da Glândula Tireoide/patologia , Neoplasias da Glândula Tireoide/cirurgia , Ultrassonografia
13.
Infect Immun ; 87(9)2019 09.
Artigo em Inglês | MEDLINE | ID: mdl-31262980

RESUMO

Pneumonia due to Gram-negative bacteria is associated with high mortality. Acinetobacter baumannii is a Gram-negative bacterium that is associated with hospital-acquired and ventilator-associated pneumonia. Bacteria have been described to release outer membrane vesicles (OMVs) that are capable of mediating systemic inflammation. The mechanism by which A. baumannii OMVs mediate inflammation is not fully defined. We sought to investigate the roles that Toll-like receptors (TLRs) play in A. baumannii OMV-mediated pulmonary inflammation. We isolated OMVs from A. baumannii cultures and intranasally introduced the OMVs into mice. Intranasal introduction of A. baumannii OMVs mediated pulmonary inflammation, which is associated with neutrophil recruitment and weight loss. In addition, A. baumannii OMVs increased the release of several chemokines and cytokines in the mouse lungs. The proinflammatory responses were partially inhibited in TLR2- and TLR4-deficient mice compared to those of wild-type mice. This study highlights the important roles of TLRs in A. baumannii OMV-induced pulmonary inflammation in vivo.


Assuntos
Infecções por Acinetobacter/microbiologia , Acinetobacter baumannii/fisiologia , Pneumonia/microbiologia , Vesículas Secretórias/fisiologia , Receptor 2 Toll-Like/fisiologia , Receptor 4 Toll-Like/fisiologia , Infecções por Acinetobacter/metabolismo , Animais , Proteínas da Membrana Bacteriana Externa , Quimiocinas/metabolismo , Citocinas/metabolismo , Modelos Animais de Doenças , Camundongos
14.
Sci Rep ; 9(1): 6849, 2019 May 02.
Artigo em Inglês | MEDLINE | ID: mdl-31048733

RESUMO

Thymosinß-4(Tß4) is an actin-sequestering protein involved in tumor malignancy. Primary cilia, microtubule-based organelles, are present in most eukaryotic cells, which might be related to tumor cell transformation. Here, we investigated whether ciliogenesis is affected by Tß4 in HeLa human cervical cancer cells. The inhibition of Tß4 attenuated primary cilia formation. The frequency of cilia was increased by Tß4 overexpression. When yeast two-hybrid assay was performed by using Tß4 as a bait, we rescued nephronophthisis 3(NPHP3), one of the components of primary cilia. Interaction of Tß4 with NPHP3 in mammalian cells was confirmed by GST-pulldown assay. Their intracellular co-localization was observed by immunofluorescence staining at peripheral surface of cells. In addition, the number of ciliated cells was reduced by the inhibition of NPHP3. Moreover, NPHP3 expression was decreased by the inhibition of Tß4 but it was increased by Tß4 overexpression. Taken together, the results demonstrate that primary cilia formation could be regulated by Tß4 through its interaction with NPHP3 and/or the control of NPHP3 expression. It suggests that Tß4 is a novel regulator for primary cilia formation by NPHP3. It also suggests that tumorigenesis could be associated with inappropriate regulation of Tß4 and/or NPHP3 expression to maintain primary cilia formation normally.

15.
Exp Mol Med ; 51(3): 34, 2019 Mar 15.
Artigo em Inglês | MEDLINE | ID: mdl-30872575
16.
Biotechnol J ; 14(1): e1800249, 2019 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-30117715

RESUMO

Recently, highly sensitive and selective biosensors have become necessary for improving public health and well-being. To fulfill this need, high-performance biosensing systems based on various nanomaterials, such as nanoparticles, carbon nanomaterials, and hybrid nanomaterials, are developed. Numerous nanomaterials show excellent physical properties, including plasmonic, magnetic, catalytic, mechanical and fluorescence properties and high electrical conductivities, and these unique and beneficial properties have contributed to the fabrication of high-performance biosensors with various applications, including in optical, electrical, and electrochemical detection platforms. In addition, these properties can be transformed to signals for the detection of biomolecules. In this review, various types of nanomaterial-based biosensors are introduced, and they show high sensitivity and selectivity. In addition, the potential applications of these sensors on the biosensing of several types of biomolecules are also discussed. These nanomaterials-based biosensing systems provide a significant improvement on healthcare including rapid monitoring and early detection of infectious disease for public health.


Assuntos
Técnicas Biossensoriais/métodos , Nanoestruturas/química , Nanopartículas Metálicas/química , Nanotubos de Carbono/química
17.
Adv Healthc Mater ; 8(4): e1801082, 2019 02.
Artigo em Inglês | MEDLINE | ID: mdl-30549424

RESUMO

Sepsis is one of the major causes of death in hospital patients and is represented by systemic inflammatory response syndrome (SIRS) associated with infection. Gram-negative bacteria including Escherichia coli can provoke sepsis by stimulating the immune systems. Outer membrane vesicles (OMVs), nanosized vesicular structures derived from Gram-negative bacteria, contain several pathogen-associated molecular patterns, and are demonstrated to mediate SIRS. Here, extracellular vesicle-mimetic ghost nanovesicles loaded with dexamethasone, an anti-inflammatory drug, are developed using alkaline solution, sonication, and buoyant density gradient ultracentrifugation. These ghost nanovesicles have comparable physical features with naturally released extracellular vesicles but have 200-fold higher production yields than extracellular vesicles. Importantly, these ghost nanovesicles are devoid of potentially unwanted luminal cargos, including cytosolic proteins and nucleic acids. By maintaining the same topology as the parental cells, these dexamethasone-loaded ghost nanovesicles derived from human U937 monocytes reduce the release of interleukin-8 from OMV-treated endothelial cells in vitro, and mitigate the symptoms of OMV-induced SIRS in vivo. This study sheds light on using extracellular vesicle-mimetic ghost nanovesicles to deliver therapeutics to treat diseases such as bacterial sepsis.

18.
Biosens Bioelectron ; 126: 425-432, 2019 Feb 01.
Artigo em Inglês | MEDLINE | ID: mdl-30471568

RESUMO

Norovirus (NoV) is a leading cause of acute gastroenteritis. The low infectious dose and environmental stability of NoV facilitate its effective transmission through a variety of modes such as food, water and person-to-person. The available enzyme-linked immunosorbent assay (ELISA) for NoV detection has low sensitivity due to the low catalytic activity of the peroxidase used, and thus, a reliable ultrasensitive bioassay is needed. In this study, we apply the enhanced peroxidase-like activity of silver ion-incorporated gold nanoparticles (Au/Ag NPs) in a colorimetric bioassay for NoV detection. NoV was captured by anti-NoV genogroup II antibodies, which were immobilized on the surface of a 96-well microtiter plate and formed a sandwich structure among anti-NoV Ab, NoV and Ab-Au NP. Then, Ag ion-containing hydroquinone was added to form Au/Ag core/shell NPs. When H2O2/3,3',5,5'-tetramethylbenzidine (TMB) solution was added to the wells, Ag ions were liberated from the surface of Au/Ag NPs and enhanced the oxidation of TMB. These reactions enhanced the oxidation of TMB and developed an intense blue color. The Au/Ag NPs were shown to exhibit higher affinity and catalytic efficiency for H2O2 and higher catalytic velocity based on the kcat of up to 7-fold compared with Au NPs. The bioassay was then optimized to detect clinically isolated NoV using NoV-like particles (NoV-LPs). NoV-LPs were detected with a limit of detection of 10.8 pg/mL, corresponding to 1000- and 100-fold higher sensitivity compared to the gold-immunoassay and horseradish peroxidase-based ELISA, respectively. Clinically isolated NoV GII.4 and NoV GII.3 were detected in the range of 102-106 copies of viral RNA/mL fecal solution with a detection limit of 13.2 copies/mL fecal solution for NoV GII.4, equivalent to 132 copies of viral RNA/g feces and indicating significantly higher sensitivity compared to commercial immunoassay kits. This bioassay represents a workable detection assay for low concentrations of NoV that is applicable for early-stage diagnosis for public hygiene.


Assuntos
Técnicas Biossensoriais , Colorimetria , Imunoensaio , Norovirus/isolamento & purificação , Catálise , Ouro/química , Peroxidase do Rábano Silvestre/química , Peróxido de Hidrogênio/química , Limite de Detecção , Nanopartículas Metálicas/química , Norovirus/patogenicidade , Prata/química
19.
Eur Radiol ; 29(2): 932-940, 2019 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-29955949

RESUMO

OBJECTS: The purpose was to determine whether the epicardial adipose tissue (EAT) area on low-dose chest CT (LDCT) could be used to predict coronary atherosclerosis in an asymptomatic population considered for lung cancer screening. METHODS: Subjects aged 55-80 years with smoking history who underwent both LDCT and coronary CT angiography (CCTA) were retrospectively enrolled. Correlation between the EAT volume in CCTA and EAT area in LDCT was evaluated. Coronary risk factors including the body surface area (BSA) indexed EAT area were compared between coronary plaque negative and positive groups. Significant factors for predicting coronary atherosclerosis were analyzed with logistic regression analysis. Receiver-operating characteristic curve analysis was performed to determine the cutoff value. RESULTS: A total of 438 subjects were enrolled, including 299 subjects with coronary atherosclerosis. There was a good correlation between the EAT volume in CCTA and EAT area in LDCT (ρ = 0.712, p < 0.001). There were significant differences in age, systolic blood pressure, all BSA indexed EAT area, sex, and hypertension between plaque negative and positive groups. In multivariate logistic regression for the BSA indexed EAT area in LDCT at the RCA level, sex (OR: 11.168, 95% CI: 2.107-59.201, p = 0.005), systolic blood pressure (OR: 1.021, 95% CI: 1.005-1.036, p = 0.009), hypertension (OR: 1.723, 95% CI: 1.103-2.753, p = 0.017), and EAT area (OR: 1.273, 95% CI: 1.154-1.405, p < 0.001) were significant. The area under the curve of the BSA indexed EAT area in LDCT at the RCA level for coronary atherosclerosis was 0.657, and the cut-off value was 7.66 cm2/m2. CONCLUSION: The EAT area in LDCT could be used to predict coronary atherosclerosis in an asymptomatic population considered for lung cancer screening. KEY POINTS: • To quantify EAT, the EAT area in LDCT can be used instead of the EAT volume in CCTA. • The EAT area measured in LDCT can be used as a predictor of coronary artery disease. • The extensive CAD group tended to have a greater EAT area than the non-extensive CAD group.


Assuntos
Tecido Adiposo/diagnóstico por imagem , Doença da Artéria Coronariana/diagnóstico por imagem , Pericárdio/diagnóstico por imagem , Tecido Adiposo/patologia , Idoso , Idoso de 80 Anos ou mais , Angiografia por Tomografia Computadorizada , Angiografia Coronária/métodos , Doença da Artéria Coronariana/patologia , Detecção Precoce de Câncer/métodos , Feminino , Humanos , Modelos Logísticos , Neoplasias Pulmonares/diagnóstico por imagem , Masculino , Pessoa de Meia-Idade , Pericárdio/patologia , Placa Aterosclerótica/diagnóstico por imagem , Placa Aterosclerótica/patologia , Curva ROC , Radiografia Torácica/métodos , Estudos Retrospectivos , Fatores de Risco , Tomografia Computadorizada por Raios X
20.
Acta Med Okayama ; 72(6): 583-589, 2018 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-30573913

RESUMO

We retrospectively evaluated the altered biomechanics of the talus in 15 adult patients (7 males, 8 females) with chronic lateral ankle instability when the ankle joint moved actively from full dorsiflexion to full plantarflexion under a non-weight bearing condition. CT images were taken for the unstable ankle and the contralateral normal (control) ankle. Three-dimensional surface models of both ankle joints were reconstructed from the CT data, and we used a computer simulation program to compare both ankle motions of inversion/eversion in the coronal plane, plantarflexion/dorsiflexion in the sagittal plane, and internal rotation/external rotation in the axial plane. This evaluation method provides in vivo, dynamic, and 3D results of ankle motion. In the ankles with chronic lateral instability and the controls, the average talar rotational movement of inversion (+)/eversion (-) was 19.0° and 15.5° and the internal rotation (+)/external rotation (-) was 30.4° and 20.7°, respectively. Paired t-tests revealed significant differences in the amount of inversion (+)/eversion (-) (p=0.012) and internal rotation (+)/external rotation (-) (p<0.001) between unstable and normal ankle joints. The difference of mean rotational movement in internal rotation (9.7°) was greater than that of inversion (3.5°). Rotational instability should be considered when evaluating chronic lateral ankle instability.


Assuntos
Articulação do Tornozelo/patologia , Instabilidade Articular/diagnóstico por imagem , Tomografia Computadorizada por Raios X , Adolescente , Adulto , Articulação do Tornozelo/fisiopatologia , Fenômenos Biomecânicos , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Estudos Retrospectivos , Adulto Jovem
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