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1.
BJU Int ; 2019 Aug 24.
Artigo em Inglês | MEDLINE | ID: mdl-31444917

RESUMO

OBJECTIVE: To evaluate the effectiveness of poloxamer-based thermo-sensitive sol-gel instillation after transurethral resection of the prostate (TURP) for preventing a urethral stricture. PATIENTS AND METHODS: A total of 198 patients underwent TURP for benign prostatic hyperplasia. Recruited patients were randomly divided into two groups: Groups A and B. Patients in group A (100 patients, experimental group) received poloxamer-based thermo-sensitive sol-gel instillation, and patients in the group B (98 patients, control group) received lubricant instillation after TURP. Each patient was evaluated at 4 weeks (V1), 12 weeks (V2), and 24 weeks (V3) after the surgery. The effectiveness of poloxamer-based thermo-sensitive sol-gel instillation was evaluated based on the International Prostate Symptom Score (IPSS)-Quality of Life (QoL), Overactive bladder questionnaire (OAB-q), peak urine flow rate (Qmax), postvoid residual volume (PVR), and cystoscopy. RESULTS: Among 198 initial participants, 80 patients in group A and 83 patients in group B had completed the experiment. There were no significant differences in IPSS-QoL and OAB-q between groups. However, Qmax (mL/sec) showed significant difference between group A and group B (18.92±9.98 vs. 15.58±9.24, p=0.028) at 24 weeks after surgery. On cystoscopic examination, urethral stricture after TURP was observed in 2 of 80 subjects in group A and 10 of 83 subjects in group B (p=0.023). CONCLUSIONS: Poloxamer-based thermo-sensitive sol-gel instillation after TURP decreased the incidence of urethral stricture. This article is protected by copyright. All rights reserved.

2.
Medicine (Baltimore) ; 98(33): e16842, 2019 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-31415408

RESUMO

INTRODUCTION: Engorgement of the epidural venous plexus (EVP) is a rare cause of nerve root impingement. Dilated epidural veins cause compression of the thecal sac and spinal nerve roots, leading to lumbar radiculopathy. PATIENT CONCERNS: Here we describe a case of severe lumbar radiculopathy in a 15-year-old morbidly obese boy. DIAGNOSIS: Enhanced lumbar magnetic resonance imaging revealed left sided L1-L2 disc protrusion and engorgement of the lumbar EVP, resulting in narrowing of the thecal sac in the entire lumbar spine. There was no evidence of an intra-abdominal mass, thrombosis of the inferior vena cava, or vascular malformation. INTERVENTIONS: A caudal epidural block was administered under fluoroscopic guidance. The patient reported a 30% reduction in pain intensity for just 1 day. OUTCOMES: The patient has been followed up for 2 years. He continues to take medication, including morphine sulfate 15 mg, gabapentin 300 mg, and oxycodone 20 mg per day. He is on a diet with exercise for weight reduction. CONCLUSION: An engorged EVP should be considered in the differential diagnosis of radiculopathy in morbidly obese patients.


Assuntos
Analgésicos Opioides/administração & dosagem , Morfina/administração & dosagem , Oxicodona/administração & dosagem , Dor Intratável/tratamento farmacológico , Radiculopatia/tratamento farmacológico , Adolescente , Anestesia Caudal/métodos , Humanos , Vértebras Lombares/diagnóstico por imagem , Imagem por Ressonância Magnética , Masculino , Morfina/uso terapêutico , Obesidade/complicações , Medição da Dor , Radiculopatia/diagnóstico por imagem
3.
Biotechnol Bioeng ; 2019 Aug 20.
Artigo em Inglês | MEDLINE | ID: mdl-31429925

RESUMO

Although in vitro models are widely accepted experimental platforms, their physiological relevance is often severely limited. The limitation of current in vitro models is strongly manifested in case of diseases where multiple organs are involved, such as diabetes and metabolic syndrome. Microphysiological systems (MPS), also known as organ-on-a-chip technology, enable a closer approximation of the human organs and tissues, by recreating the tissue microenvironment. Multiorgan MPS, also known as multiorgan-on-a-chip or body-on-a-chip, offer the possibility of reproducing interactions between organs by connecting different organ modules. Here, we designed a three-organ MPS consisting of pancreas, muscle, and liver, to recapitulate glucose metabolism and homeostasis by constructing a mathematical model of glucose metabolism, based on experimental measurement of glucose uptake by muscle cells and insulin secretion by pancreas cells. A mathematical model was used to modify the MPS to improve the physiological relevance, and by adding the liver model in the mathematical model, physiological realistic glucose and insulin profiles were obtained. Our study may provide a methodological framework for developing multiorgan MPS for recapitulating the complex interaction between multiple organs.

4.
Artigo em Inglês | MEDLINE | ID: mdl-31408161

RESUMO

CONTEXT: Bidirectional relationship between low HDL-cholesterol (HDL-C) and glucose intolerance is well established. Recent studies suggested an association of lipid variability with various health outcomes. OBJECTIVE: To investigate the combined effect of HDL-C levels and their variability on the risk of diabetes. DESIGN: A population-based cohort study. SETTING AND PARTICIPANTS: 5,114,735 adults without known diabetes who underwent ≥3 health examinations from 2009 to 2013 in the Korean National Health Insurance System cohort were included. Visit-to-visit HDL-C variability was calculated using the variability independent of the mean (VIM) and the coefficient of variation (CV). Low mean and high variability groups were defined as the lowest and the highest quartile of HDL-C mean and variability, respectively. MAIN OUTCOME MEASURES: Newly-developed diabetes. RESULTS: There were 122,192 cases (2.4%) of incident diabetes during the median follow-up of 5.1 years. Lower mean or higher variability of HDL-C were associated with higher risk of diabetes in a stepwise manner, and an additive effect of the two measures was noted. In the multivariable adjusted model, the hazard ratios and 95% CI for incident diabetes were 1.20 (1.18-1.22) in high mean/high VIM group, 1.35 (1.33-1.37) in low mean/low VIM group and 1.40 (1.38-1.42) in low mean/high VIM group compared with high mean/low VIM group. Similar results were observed when modeling the variability using CV and in various subgroup analyses. CONCLUSIONS: Low mean and high variability in HDL-C were independent predictors of diabetes with an additive effect. Both elevating and stabilizing HDL-C might be an important goal for the reduction of diabetes risk.

5.
World Neurosurg ; 2019 Aug 02.
Artigo em Inglês | MEDLINE | ID: mdl-31382068

RESUMO

OBJECTIVE: Microvascular decompression (MVD) is the treatment of choice for hemifacial spasm (HFS), due to the high rate of complete resolution associated with MVD. However, some patients experience recurrent or persistent symptoms after surgery. In this study, we evaluated the causes of recurrence or failure based on our surgical experience with revision of MVD for HFS and analyzed relationship between surgical outcomes and radiological and intraoperative findings. MATERIAL AND METHODS: Among more than 2500 patients who underwent MVD surgery for HFS, 23 patients received a second MVD in our hospital from January 2002 to December 2017. Three-dimensional time-of-flight magnetic resonance angiography and reconstructed imaging were used to identify the culprit vessel and its conflict upon root exit zone (REZ) of the facial nerve. We reviewed patients' medical records and operation videos to identify the missing points of first surgery. RESULTS: In our experience with revision of MVD, 8 patients had incomplete decompression, such as single-vessel decompression of multiple offending vessels. Teflon was not detected at the REZ, but was found in other locations in 12 patients. Three patients had severe adhesion with previous Teflon around the REZ. Nineteen patients had excellent surgical outcomes at immediate postoperative evaluation; 20 patients showed spasm disappearance at 1 year after surgery and 3 patients showed persistent symptoms. Neuro-vascular contacts around REZ of facial nerve were revealed on MRI of incomplete decompression and Teflon malposition patient groups. There were no clear neuro-vascular contacts in the patients with severe Teflon adhesion. CONCLUSION: The decision on secondary MVD for persistent or recurrent spasm is troubling. However, if the neurovascular contact was observed in the MRI of the patient and there were offending vessels, the surgical outcome might be favorable.

6.
BMC Genet ; 20(1): 66, 2019 Aug 05.
Artigo em Inglês | MEDLINE | ID: mdl-31382890

RESUMO

BACKGROUND: The Sapsaree is a breed of dog (Canis familiaris) native to Korea, which became perilously close to extinction in the mid-1980s. However, with systematic genetic conservation and restoration efforts, this breed was rescued from extinction and population sizes have been gradually increasing over the past few decades. The aim of this study was to ascertain novel information about the genetic diversity, population structure, and demographic history of the Sapsaree breed using genome-wide single nucleotide polymorphism data. We characterized the genetic profile of the Sapsaree breed by comparison with seven foreign dog breeds with similar morphologies to estimate genetic differentiation within and among these breeds. RESULTS: The results suggest that Sapsarees have higher genetic variance compared with the other breeds analyzed. The majority of the Sapsarees in this study share a discrete genetic pattern, although some individuals were slightly different, possibly as a consequence of the recent restoration process. Concordant results from analyses of linkage disequilibrium, effective population size, genetic diversity, and population structural analyses illustrate a relationship among the Sapsaree and the Tibetan breeds Tibetan terrier and Lhasa Apso, and a small genetic introgression from European breeds. The effective population size of the Sapsaree has contracted dramatically over the past generations, and is currently insufficient to maintain long-term viability of the breed's genetic diversity. CONCLUSIONS: This study provides novel insights regarding the genetic diversity and population structure of the native Korean dog breed Sapsaree. Our results suggest the importance of a strategic and systematic approach to ensure the genetic diversity and the authenticity of the Sapsaree breed.

7.
Mol Cancer Ther ; 2019 Jul 15.
Artigo em Inglês | MEDLINE | ID: mdl-31308079

RESUMO

Triple negative breast cancer (TNBC) accounts disproportionally for the majority of breast cancer related deaths throughout the world. This is largely attributed to lack of a specific therapy capable of targeting both bulk tumor mass and cancer stem cells (CSC) as well as appropriate animal models to accurately evaluate treatment efficacy for clinical translation. Thus, development of effective and clinically translatable targeted therapies for TNBC is an unmet medical need. We developed a hybrid nanoparticles-based co-delivery platform containing both paclitaxel and verteporfin (PV-NPs) to target TNBC patient-derived xenograft (PDX) tumor and CSCs. MRI and IVIS imaging were performed on mice containing PDX tumors to assess tumor vascularity and accumulation of NPs. NF-kB, Wnt and YAP activities were measured by reporter assays. Mice bearing TNBC PDX tumor were treated with PV-NPs and controls, and tumors progression and CSC subpopulations were analyzed. MRI imaging indicated high vascularization of PDX tumors. IVIS imaging showed accumulation of NPs in PDX tumors. In comparison to control-NPs and free-drug combination, PV-NPs significantly retarded tumor growth of TNBC PDX. PV-NPs simultaneously repressed NF-kB, Wnt and YAP that have been shown to be crucial for cancer growth, CSC development and tumorigenesis. In conclusion, NPs containing two clinically used drugs concurrently inhibited NF-kB, Wnt and YAP pathways and exhibited synergic effects on killing TNBC bulk tumor and CSCs. This combination nanotherapy evaluated with a PDX model may lead to an effective treatment of TNBC patients.

8.
Liver Transpl ; 2019 Jul 04.
Artigo em Inglês | MEDLINE | ID: mdl-31271699

RESUMO

Donor safety and graft results of pure laparoscopic living donor right hepatectomy (LLDRH) have previously been compared to those of open living donor right hepatectomy (OLDRH). However, the clinical outcomes of recipients at 1-year follow-up have never been accurately compared. We aimed to compare 1-year outcomes of recipients of living donor right liver transplantation (LRLT) using pure LLDRH and OLRDH. From May 2013 to May 2017, 197 consecutive recipients underwent LRLT. Donor hepatectomies were performed either by OLRDH (n = 127) or pure LLDRH (n = 70). After propensity score matching, 53 recipients were included in each group for analysis. The clinical outcomes at 1-year follow-up were compared between the two groups. The primary outcome was recipient death or graft failure during 1-year follow-up. In propensity-matched analysis, the incidence of death or graft failure during 1-year follow-up was not different between the two groups (3.8% vs. 5.7% odds ratio [OR], 1.45; 95% confidence interval [CI], 0.24-8.95; P = 0.69). However, the composite of Clavien Dindo IIIb-V complications was more frequent in the pure LLDRH group (OR, 2.62; 95% CI, 1.15-5.96; P = 0.02). CONCLUSION: Although pure LLDRH affords a comparable incidence of fatal complications in recipients, operative complications may increase at the beginning of the program. The safety of the recipients should be confirmed to accept pure LLDRH as a feasible option. This article is protected by copyright. All rights reserved.

9.
Transplantation ; 2019 Jun 24.
Artigo em Inglês | MEDLINE | ID: mdl-31283680

RESUMO

BACKGROUND: The innovative pure laparoscopic living donor right hepatectomy (LLDRH) procedure for liver transplantation has never been fully compared to open living donor right hepatectomy (OLRDH). We aimed to compare the donor safety and graft results of pure LLDRH to those of OLRDH. METHODS: From May 2013 to July 2017, 288 consecutive donors underwent either OLRDH (n = 197) or pure LLDRH (n = 91). After propensity score matching, 72 donors were included in each group. The primary outcome was postoperative complications during a 90-day follow-up period. Comprehensive complication index, duration of hospital stay, need for additional pain control, readmission, and donor outcomes were also compared. RESULTS: The incidence of major complication during the 90-day follow-up was higher in the OLDRH group than the LLDRH group (6.6% vs. 15.4%, P = 0.017) but was not statistically significant in propensity-matched analysis (11.1% vs. 13.9%, odds ratio [OR], 1.29; 95% confidence interval [CI], 0.47-3.51; P = 0.62). A right hepatic duct less than 1 cm was independently associated with complication in the pure LLDRH group (OR, 4.01; 95% CI, 1.08-14.99; P = 0.04). CONCLUSION: In the initial 91 pure LLDRH cases, incidence of major complication was higher than in the OLDRH group, but the difference was not significant in propensity-matched analysis. A right hepatic duct verified as less than 1 cm may be related to increased frequency of complications in pure LLDRH donors. Further analysis is needed.

10.
Heart Rhythm ; 2019 Jul 09.
Artigo em Inglês | MEDLINE | ID: mdl-31299298

RESUMO

BACKGROUND: The variability of metabolic parameters might have an impact on the pathophysiology of atrial fibrillation (AF). OBJECTIVE: The purpose of this study was to evaluate the effect of the variability of 4 metabolic components including systolic blood pressure (BP), glucose level, total cholesterol (TC) level, and body mass index (BMI) on the risk of AF in the healthy population without hypertension, diabetes, or dyslipidemia. METHODS: We identified 6,819,829 adult subjects without hypertension, diabetes, or dyslipidemia who had ≥3 health checkups provided by the Korean National Health Insurance Corporation between 2005 and 2012. Glucose level, BP, TC level, and BMI were measured at each visit. Variability was defined as variability independent of the mean (VIM), and VIM of each parameter was divided into 4 groups. High variability was defined as having values in the highest quartile of each parameter. RESULTS: During a mean follow-up of 5.3 ± 1.1 years, 31,302 subjects were newly diagnosed with AF (0.86 per 1000 person-years). Subjects with the highest VIM quartile of BP, TC level, and BMI showed an increased risk of AF compared with those with the lowest VIM quartile, whereas glucose level variability had a marginal association. The composite of the high variability of metabolic parameters showed a graded risk of AF. After multivariable adjustment, subjects having 1, 2, 3, and 4 parameters of the highest VIM had an ∼7%, 13%, 20%, and 35% increased risk of AF compared with those without any highest variability of metabolic parameters. CONCLUSION: The variability of metabolic parameters showed a close association with the risk of AF in those without cardiovascular comorbidities.

11.
PLoS One ; 14(7): e0219978, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31329612

RESUMO

X-box binding protein 1 (XBP1) mRNA processing plays a crucial role in the unfolded protein response (UPR), which is activated in response to endoplasmic reticulum (ER) stress. Upon accumulation of the UPR-converted XBP1 mRNA splicing from an unspliced (u) XBP1 (inactive) isoform to the spliced (s) XBP1 (active) isoform, inositol-requiring enzyme 1 α (IRE1α) removes a 26-nucleotide intron from uXBP1 mRNA. Recent studies have reported the assessment of ER stress by examining the ratio of sXBP1 to uXBP1 mRNA (s/uXBP1 ratio) via densitometric analysis of PCR bands relative to increased levels of sXBP1 to uXBP1 using a housekeeping gene for normalization. However, this measurement is visualized by gel electrophoresis, making it very difficult to quantify differences between the two XBP1 bands and complicating data interpretation. Moreover, most commonly used housekeeping genes display an unacceptably high variable expression pattern of the s/uXBP1 ratio under different experimental conditions, such as various phases of development and different cell types, limiting their use as internal controls. For a more quantitative determination of XBP1 splicing activity, we measured the expression levels of total XBP1 (tXBP1: common region of s/uXBP1) and sXBP1 via real-time PCR using specific primer sets. We also designed universal real-time PCR primer sets capable of amplifying a portion of each u/s/tXBP1 mRNA that is highly conserved in eukaryotes, including humans, monkeys, cows, pigs, and mice. Therefore, we provide a more convenient and easily approachable quantitative real-time PCR method that can be used in various research fields to assess ER stress.

12.
ACS Nano ; 13(6): 7146-7154, 2019 Jun 25.
Artigo em Inglês | MEDLINE | ID: mdl-31180627

RESUMO

Grafting nanotechnology on thermoelectric materials leads to significant advances in their performance. Creation of structural defects including nano-inclusion and interfaces via nanostructuring achieves higher thermoelectric efficiencies. However, it is still challenging to optimize the nanostructure via conventional fabrication techniques. The thermal instability of nanostructures remains an issue in the reproducibility of fabrication processes and long-term stability during operation. This work presents a versatile strategy to create numerous interfaces in a thermoelectric material via an atomic-layer deposition (ALD) technique. An extremely thin ZnO layer was conformally formed via ALD over the Bi0.4Sb1.6Te3 powders, and numerous heterogeneous interfaces were generated from the formation of Bi0.4Sb1.6Te3-ZnO core-shell structures even after high-temperature sintering. The incorporation of ALD-grown ZnO into the Bi0.4Sb1.6Te3 matrix blocks phonon propagation and also provides tunability in electronic carrier density via impurity doping at the heterogeneous grain boundaries. The exquisite control in the ALD cycles provides a high thermoelectric performance of zT = 1.50 ± 0.15 (at 329-360 K). Specifically, ALD is an industry compatible technique that allows uniform and conformal coating over large quantities of powders. The study is promising in terms of the mass production of nanostructured thermoelectric materials with considerable improvements in performance via an industry compatible and reproducible route.

13.
Int J Stem Cells ; 2019 Jun 30.
Artigo em Inglês | MEDLINE | ID: mdl-31242719

RESUMO

Background and Objectives: Proficient differentiation of human pluripotent stem cells (hPSCs) into specific lineages is required for applications in regenerative medicine. A growing amount of evidences had implicated hormones and hormone-like molecules as critical regulators of proliferation and lineage specification during in vivo development. Therefore, a deeper understanding of the hormones and hormone-like molecules involved in cell fate decisions is critical for efficient and controlled differentiation of hPSCs into specific lineages. Thus, we functionally and quantitatively compared the effects of diverse hormones (estradiol 17-ß (E2), progesterone (P4), and dexamethasone (DM)) and a hormone-like molecule (retinoic acid (RA)) on the regulation of hematopoietic and neural lineage specification. Methods and Results: We used 10 nM E2, 3 µM P4, 10 nM DM, and 10 nM RA based on their functional in vivo developmental potential. The sex hormone E2 enhanced functional activity of hematopoietic progenitors compared to P4 and DM, whereas RA impaired hematopoietic differentiation. In addition, E2 increased CD34+CD45+ cells with progenitor functions, even in the CD43- population, a well-known hemogenic marker. RA exhibited lineage-biased potential, preferentially committing hPSCs toward the neural lineage while restricting the hematopoietic fate decision. Conclusions: Our findings reveal unique cell fate potentials of E2 and RA treatment and provide valuable differentiation information that is essential for hPSC applications.

14.
Sci Rep ; 9(1): 8952, 2019 Jun 20.
Artigo em Inglês | MEDLINE | ID: mdl-31222158

RESUMO

Ni-rich layered LiNi0.84Co0.10Mn0.06O2 cathode material was modified by doping with vanadium to enhance the electrochemical performances. The XRD, FESEM and XPS analyses were indicated that the vanadium is successfully doped in the crystal lattice of LiNi0.84Co0.10Mn0.06O2 with high crystallinity. 0.05 mol% vanadium doped LiNi0.84Co0.10Mn0.06O2 exhibits superior initial discharge capacity of 204.4 mAh g-1, cycling retention of 88.1% after 80 cycles and rate capability of 86.2% at 2 C compared to those of pristine sample. It can be inferred that the vanadium doping can stabilize the crystal structure and improve the lithium-ion kinetics of the layered cathode materials.

15.
Diabetes Metab J ; 2019 Jun 19.
Artigo em Inglês | MEDLINE | ID: mdl-31237133

RESUMO

BACKGROUND: We aimed to investigate the effectiveness and safety of adding basal insulin to initiating dipeptidyl peptidase-4 (DPP-4) inhibitor and metformin and/or sulfonylurea (SU) in achieving the target glycosylated hemoglobin (HbA1c) in patients with type 2 diabetes mellitus (T2DM). METHODS: This was a single-arm, multicenter, 24-week, open-label, phase 4 study in patients with inadequately controlled (HbA1c ≥7.5%) T2DM despite the use of DPP-4 inhibitor and metformin. A total of 108 patients received insulin glargine while continuing oral antidiabetic drugs (OADs). The primary efficacy endpoint was the percentage of subjects achieving HbA1c ≤7.0%. Other glycemic profiles were also evaluated, and the safety endpoints were adverse events (AEs) and hypoglycemia. RESULTS: The median HbA1c at baseline (8.9%; range, 7.5% to 11.1%) decreased to 7.6% (5.5% to 11.7%) at 24 weeks. Overall, 31.7% subjects (n=33) achieved the target HbA1c level of ≤7.0%. The mean differences in body weight and fasting plasma glucose were 1.2±3.4 kg and 56.0±49.8 mg/dL, respectively. Hypoglycemia was reported in 36 subjects (33.3%, 112 episodes), all of which were fully recovered. There was no serious AE attributed to insulin glargine. Body weight change was significantly different between SU users and nonusers (1.5±2.5 kg vs. -0.9±6.0 kg, P=0.011). CONCLUSION: The combination add-on therapy of insulin glargine, on metformin and DPP-4 inhibitors with or without SU was safe and efficient in reducing HbA1c levels and thus, is a preferable option in managing T2DM patients exhibiting dysglycemia despite the use of OADs.

16.
J Nanosci Nanotechnol ; 19(12): 7721-7728, 2019 Dec 01.
Artigo em Inglês | MEDLINE | ID: mdl-31196281

RESUMO

In this study, the morphological effects of ZnO on the antimicrobial and deodorant activities of synthetic fibers were investigated. Three different polyethylene terephthalate (PET)/ZnO filaments were prepared by incorporating various ZnO nanostructures (rods, plates, and spheres) into PET filaments via a melt-spinning process. The antimicrobial activity of the as-prepared fibers was evaluated by the shake-flask method using two types of bacteria (Staphylococcus aureus and Klebsiella pneumoniae). The deodorant activity of the as-prepared fibers was evaluated by the gas detection tube method. All the PET/ZnO filaments exhibited excellent antimicrobial activity with a bacterial reduction value of 99.9%. The PET/ZnO rod filament showed the best deodorant performance of 60.0%. Both the antimicrobial and deodorant activities of the PET/ZnO filaments were influenced by the morphology of ZnO. However, the morphology of ZnO had a different effect on each functionality of the PET/ZnO filaments. The antimicrobial activity of the PET/ZnO filaments was mainly affected by the physical properties of ZnO rather than its morphology. By contrast, the deodorant activity of the PET/ZnO filaments was highly influenced by the morphology of ZnO.

17.
Int J Cardiol ; 293: 153-158, 2019 Oct 15.
Artigo em Inglês | MEDLINE | ID: mdl-31253527

RESUMO

BACKGROUND: This study examined the effects of variability of four metabolic parameters, namely systolic blood pressure (BP), body mass index (BMI), fasting blood glucose (FBG), and total cholesterol level (TC) on the risk of HF. The effects of metabolic parameter variability on the risk of heart failure (HF) remain unclear. METHODS: We studied individuals aged ≥40 years who had undergone ≥3 health check-ups under the Korean National Health Insurance Corporation during 2009 and 2012, and those who did not have hypertension, diabetes, or dyslipidemia. BP, BMI, FBG, and TC were measured at every visit. We defined the variability of each parameter using the variability independent of the mean (VIM) method. VIMs were categorized into four groups according to quartiles. The metabolic variability (MV) score for each subject was defined as the number of VIMs in the highest quartile. RESULTS: Among the 3,820,191 subjects, 17,253 (0.45%) had incident HF during a mean 5.3 ±â€¯1.1 years of follow-up. High variability of each parameter was associated with increased HF risk, which increased according to the MV score. After multivariable adjustment, compared to subjects with MV score = 0, subjects with MV score = 1-4 had an increased risk of HF (adjusted HR [95% CI], 1.15 [1.10-1.19] for MV score = 1, 1.33 [1.28-1.39] for MV score = 2, 1.48 [1.40-1.57] for MV score = 3, 1.74 [1.55-1.96] for MV score = 4 [p-for-trend ≪0.0001]). CONCLUSIONS: High variability of BP, BMI, FBG, and TC was synergistically associated with a higher incidence of new-onset HF.

18.
Sci Rep ; 9(1): 8901, 2019 Jun 20.
Artigo em Inglês | MEDLINE | ID: mdl-31222121

RESUMO

We report high electrochemical performances of LiNi0.91Co0.06Mn0.03O2 cathode material for high-energy lithium ion batteries. LiNi0.91Co0.06Mn0.03O2 is synthesized at various sintering temperatures (640~740 °C). The sintering temperatures affect crystallinity and structural stability, which play an important role in electrochemical performances of LiNi0.91Co0.06Mn0.03O2. The electrochemical performances are improved with increasing sintering temperature up to an optimal sintering temperature. The LiNi0.91Co0.06Mn0.03O2 sintered at 660 °C shows remarkably excellent performances such as initial discharge capacity of 211.5 mAh/g at 0.1 C, cyclability of 85.3% after 70 cycles at 0.5 C and rate capability of 90.6% at 2 C as compared to 0.5 C. These results validate that LiNi0.91Co0.06Mn0.03O2 sintered at 660 °C can be regarded as a next generation cathode.

19.
J Natl Compr Canc Netw ; 17(5): 441-449, 2019 May 01.
Artigo em Inglês | MEDLINE | ID: mdl-31085754

RESUMO

BACKGROUND: We conducted a comparative survival analysis between primary androgen deprivation therapy (PADT) and radical prostatectomy (RP) based on nationwide Korean population data that included all patients with prostate cancer. MATERIALS AND METHODS: This study enrolled 4,538 patients with prostate cancer from the National Health Insurance Service (NHIS) database linked with Korean Central Cancer Registry data who were treated with PADT or RP between January 1, 2007, and December 31, 2014. Kaplan-Meier and multivariate survival analyses stratified by stage (localized and locally advanced) and age (<75 and ≥75 years) were performed using a Cox proportional hazards model to evaluate treatment effects. RESULTS: Among 18,403 patients from the NHIS database diagnosed with prostate cancer during the study period, 4,538 satisfied inclusion criteria and were included in the analyses. Of these, 3,136 and 1,402 patients underwent RP or received PADT, respectively. Risk of death was significantly increased for patients who received PADT compared with those who underwent RP in the propensity score-matched cohort. In subgroup analyses stratified by stage and age, in every subgroup, patients who received PADT had a significantly increased risk of death compared with those who underwent RP. In particular, a much greater risk was observed for patients with locally advanced prostate cancer. CONCLUSIONS: Based on a nationwide survival analysis of nonmetastatic prostate cancer, this study provides valuable clinical implications that favor RP over PDAT for treatment of Asian populations. However, the possibility that survival differences have been overestimated due to not accounting for potential confounding characteristics must be considered.

20.
Front Immunol ; 10: 879, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31105701

RESUMO

Background: Natural Killer (NK) cell-based immunotherapy used to treat cancer requires the adoptive transfer of a large number of activated NK cells. Here, we report a new effective method to expand human NK cells ex vivo using K562 cells genetically engineered (GE) to express OX40 ligand (K562-OX40L) in combination with a short exposure to soluble IL-21. In addition, we describe a possible mechanism of the NK cell expansion through the OX40 receptor-OX40 ligand axis which is dependent on NK cell homotypic interaction. Methods: K562-OX40L cells were generated by lentiviral transduction and were used as feeder cells to expand and activate NK cells from PBMCs in the presence of IL-2/IL-15. Soluble IL-21 was also added in various concentrations only once at the beginning of the culture. NK cells were expanded for 4-5 weeks, and the purity, expansion rate, phenotype and function (cytotoxicity, antibody-dependent cell-mediated cytotoxicity (ADCC), cytokine production, CD107a degranulation) of these expanded NK cells were compared to those generated by using K562 feeder cells. Results: The culture of NK cells with K562-OX40L cells in combination with the transient exposure to IL-21 highly enhanced NK cell expansion to approximately 2,000-fold after 4 weeks of culture, compared to a 303-fold expansion using the conventional K562 cells. Mechanistically, the OX40-OX40L axis between the feeder cells and NK cells as well as the homotypic interaction between NK cells through the OX40-OX40L axis were both necessary for NK cell expansion. The short exposure of NK cells to IL-21 had a synergistic effect with OX40 signaling for NK cell expansion. Apart from their enhanced expansion, NK cells grown with K562-OX40L feeder cells were similar to those grown with conventional K562 cells in regard to the surface expression of various receptors, cytotoxicity, ADCC, cytokine secretion, and CD107 degranulation. Conclusion: Our data suggest that OX40 ligand is a potent co-stimulant for the robust expansion of human NK cells and the homotypic NK cell interactions through the OX40-OX40L axis is a mechanism of NK cell expansion.

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