Your browser doesn't support javascript.
loading
Mostrar: 20 | 50 | 100
Resultados 1 - 19 de 19
Filtrar
Mais filtros










Base de dados
Intervalo de ano de publicação
1.
J Hazard Mater ; 424(Pt A): 127322, 2021 Sep 26.
Artigo em Inglês | MEDLINE | ID: mdl-34601407

RESUMO

Soil contamination due to chlorinated organics prompts an important environmental problem; however, the iron-based reduction materials and complicated ground environment are the main barriers to implementation and promotion of in situ soil remediation. Therefore, this study aims to evaluate the reductants zero-valent iron (ZVI) and its activated carbon composite (AC-ZVI) in terms of their self-oxidation and selectivity in soil experiments. The results indicated that saturated moisture conditions were beneficial for degradation due to the dispersal of the pollutants from soil particles. Particularly, increasing the water/soil ratio to the over-saturated state would decrease the selectivity of ZVI and AC-ZVI. Meanwhile, increasing the reductant loading decreased the selectivity of ZVI and AC-ZVI, whereas the high initial concentration increased the selectivity of AC-ZVI. In addition, the self-oxidation of ZVI (3.0 ×10-3 h-1) is 4.2 times higher than that of AC-ZVI (0.7 ×10-3 h-1), and the selectivity of AC-ZVI (48%) is 6.9 times higher than that of ZVI (7%), which confirmed that AC-ZVI is a superior iron-based amendment in saturated moisture conditions. Therefore, this study provides a reliable and feasible evaluation method for in situ remediation process, and deepens the understanding of the effects of moisture contents.

2.
Nutrients ; 13(7)2021 Jul 17.
Artigo em Inglês | MEDLINE | ID: mdl-34371956

RESUMO

We examined the immunomodulatory and anti-inflammatory effects of asiatic acid (AA) in atopic dermatitis (AD). AA treatment (5-20 µg/mL) dose-dependently suppressed the tumor necrosis factor (TNF)-α level and interleukin (IL)-6 protein expression in interferon (IFN)-γ + TNF-α-treated HaCaT cells. The 2,4-dinitrocholrlbenzene (DNCB)-induced AD animal model was developed by administering two AA concentrations (30 and 75 mg/kg/d: AD + AA-L and AD + AA-H groups, respectively) for 18 days. Interestingly, AA treatment decreased AD skin lesions formation and affected other AD characteristics, such as increased ear thickness, lymph node and spleen size, dermal and epidermal thickness, collagen deposition, and mast cell infiltration in dorsal skin. In addition, in the DNCB-induced AD animal model, AA treatment downregulated the mRNA expression level of AD-related cytokines, such as Th1- (TNF-α and IL-1ß and -12) and Th2 (IL-4, -5, -6, -13, and -31)-related cytokines as well as that of cyclooxygenase-2 and CXCL9. Moreover, in the AA treatment group, the protein level of inflammatory cytokines, including COX-2, IL-6, TNF-α, and IL-8, as well as the NF-κB and MAPK signaling pathways, were decreased. Overall, our study confirmed that AA administration inhibited AD skin lesion formation via enhancing immunomodulation and inhibiting inflammation. Thus, AA can be used as palliative medication for regulating AD symptoms.


Assuntos
Anti-Inflamatórios não Esteroides/farmacologia , Citocinas/metabolismo , Dermatite Atópica/tratamento farmacológico , Fatores Imunológicos/farmacologia , Triterpenos Pentacíclicos/farmacologia , Animais , Anti-Inflamatórios não Esteroides/administração & dosagem , Anti-Inflamatórios não Esteroides/uso terapêutico , Linhagem Celular , Sobrevivência Celular , Colágeno/análise , Citocinas/genética , Dermatite Atópica/imunologia , Dermatite Atópica/patologia , Derme/patologia , Dinitroclorobenzeno , Modelos Animais de Doenças , Epiderme/patologia , Feminino , Regulação da Expressão Gênica , Humanos , Fatores Imunológicos/administração & dosagem , Fatores Imunológicos/uso terapêutico , Imunomodulação , Tecido Linfoide/patologia , Sistema de Sinalização das MAP Quinases/efeitos dos fármacos , Mastócitos , Camundongos , Camundongos Endogâmicos BALB C , NF-kappa B/metabolismo , Tamanho do Órgão/efeitos dos fármacos , Triterpenos Pentacíclicos/uso terapêutico , Proteínas Proto-Oncogênicas c-akt/metabolismo , Transdução de Sinais/efeitos dos fármacos
3.
Chemosphere ; 284: 131311, 2021 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-34182283

RESUMO

Perfluorooctanoic acid (PFOA) is a carcinogen with a high binding energy between fluorine and carbon and is symmetrically linked, making it difficult to treat. In this study, a self-doped TiO2 nanotube array (TNTA) was used as the anode and platinum as the cathode to quantify the PFOA removal mechanism using a photoelectrochemical (PEC) system. The external voltage was negative compared to that of the anode. In addition, NO3- and t-BuOH were used as scavengers to quantify the PFOA oxidation/reduction mechanism in the PEC system. As a result of the study, TNTA crystals are TiO2 anatase, and the band gap energy was 3.42. The synergy index of PEC was 1.25, and the best electrolyte was SO42-. The PFOA decomposition activation energy corresponds to 70.84 kJ mol-1. Moreover, ΔH# and ΔS# correspond to 68.34 kJ mol-1 and 0.190 kJ mol-1 K-1, respectively. When the external negative voltage was 1 V, the contributions of the oxidation/reduction reaction during PFOA decomposition were 60% and 40%, and when the external negative voltage was 5 V, the contributions of the redox reaction were 45% and 55%. As the external negative voltage increased, the contribution of the reduction reaction increased as the number of electrons applied to the anode increased. When PFOA was decomposed, the by-products were C7F13O2H, C6F11O2H, C5F9O2H, and C4F7O2H, respectively. This study is expected to be used as basic data for research on the effects of other factors on the oxidation/reduction as well as the selection of anode and cathode materials on the decomposition of pollutants other than PFOA when using a PEC system.


Assuntos
Caprilatos , Fluorcarbonetos , Eletrodos , Oxirredução
4.
Chemosphere ; 279: 130523, 2021 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-33865163

RESUMO

Rare earth (RE) containing radioactive species and a variety of toxic pollutants The treatment of real wastewater is very important for environmental protection. In this study, a new concept of continuous process consisting of precipitation, adsorption, and oxidation was developed without the use of chemicals. In the sedimentation step, waste oyster shell(WOS) and a PE tube diffuser(PE250) containing Na2S (PECa/S), PECa/S were prepared, which were used to precipitate heavy metals with a removal efficiency of 97% or more. In the adsorption step, fluorine (F), arsenic (As), and thorium (Th) were precipitated and removed when heavy metals were removed using coal mine drainage sludge (CMDS) and an adsorbent (PUCMDS) made of polyurethane (PU). Running a semi and pilot scale continuous process using PECa/S, PUCMDS and O3/HC systems resulted in a semi and pilot scale operating period of 120 and 62 days, and 60.26, 797.84, 46.94, 78.62 g, and 7.120 kg and 266.35, 42556.8, 191.95, 3108.43 g and 629.84 kg for As, F, Th, Pb and CODcr has been removed respectively. In addition, the removal efficiencies of As, F, Th, Pb and CODcr were 99.75, 99.98, 93.60, 99.99, and 88.82%, respectively, when treating real RE wastewater using the pilot scale system. Without the use of agitated reactors and regulators, the new concept of continuous process can effectively treat RE real wastewater, and the quality of the process outlet has met the pollutant limits recommended by EPA and China for irrigation.


Assuntos
Metais Pesados , Metais Terras Raras , Poluentes Químicos da Água , Purificação da Água , Adsorção , China , Metais Pesados/análise , Esgotos , Eliminação de Resíduos Líquidos , Águas Residuárias , Poluentes Químicos da Água/análise
5.
Environ Pollut ; 275: 116617, 2021 Apr 15.
Artigo em Inglês | MEDLINE | ID: mdl-33556734

RESUMO

Soil contaminated with toxic heavy metals (THMs) was stabilized by adding a combination of waste resources in 7.0 wt%, including coal-mine drainage sludge, waste cow bone, and steelmaking slag, in the ratio of 5:35:60. Subsequently, corn and peanut were cultivated in treated soil to investigate the effects of the waste resources on THM mobility in soil and translocation to plants. Sequential extraction procedures (SEP) was used to analyze mobile phase THMs which could be accumulated in the plants. SEP shows that mobile Pb, Cd, Cu, Zn, Ni, Cr, and As were reduced by 8.48%, 29.22%, 18.85%, 21.66%, 4.58%, 62.78%, and 20.01%, respectively. The bioaccumulation of THMs was clearly hindered by stabilization; however, the increment in the amount of immobile-phase THMs and change in the amount of translocated THMs was not proportional. The corn grains grown above the soil surface were compared with the peanut grains grown beneath the soil surface, and the results indicating that the efficiency of stabilization on THM translocation may not depend on the contact of grain to soil but the nature of plant. Interestingly, the results of bioaccumulation with and without stabilization showed that the movement of some THMs inside the plants was affected by stabilization.


Assuntos
Metais Pesados , Poluentes do Solo , Arachis , Metais Pesados/análise , Solo , Poluentes do Solo/análise , Zea mays
6.
Chemosphere ; 272: 129560, 2021 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-33460828

RESUMO

The aim of this study was to evaluate the performance of a new adsorbent in terms of beading the sludge generated from coal mine drainage or arsenic removed from water is treated by electro-purification (EP) and chemical-precipitation (CP) methods. Batch experiments were conducted to study the influence of experimental parameters such as pH and temperature, as well as the mechanism of arsenic adsorption with the new adsorbent. The porosity of coal mine drainage sludge (CMDS)-beaded adsorbent made of chitosan and alginate was optimized by adding NaHCO3 powder to generate CO2 gas during the preparation process. Two types of adsorbents, beaded EP Najeon CMDS (BCMDSEP-NJ) and beaded CP Yeongdong CMDS (BCMDSCP-YD), were prepared by heating. The specific surface areas of the powdered adsorbents CMDSEP-NJ and CMDSCP-YD were 104 and 231 m2 g-1, respectively. The prepared beaded adsorbents BCMDSEP-NJ and BCMDSCP-YD had good porosity and specific surface areas of 16.8 and 21.2 m2 g-1, respectively. The X-ray diffraction results showed that the structure was goethite (aragonite) and schwertmannite. The pseudo second-order, intra-particle, and Langmuir models were used to explain the adsorption process. The qmax values of As(III) with BCDMSEP-NJ and BCMDSCP-YD adsorbents are 4.31 and 4.58 mg g-1, respectively and those of AS(V) are 9.31 and 10.93 mg g-1, respectively. The adsorption capacity for As(III) increased with increasing pH, whereas that for As(V) decreased. The activation energy was 8 kJ mol-1 or more. The mechanism of adsorption of arsenic using a beaded adsorbent was chemical adsorption followed by diffusion. The results of the present study suggest that new adsorbents can be effectively utilized for arsenic removal from water.


Assuntos
Arsênio , Poluentes Químicos da Água , Purificação da Água , Adsorção , Carvão Mineral , Concentração de Íons de Hidrogênio , Cinética , Pós , Esgotos , Poluentes Químicos da Água/análise
7.
Ultrason Sonochem ; 72: 105412, 2021 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-33316732

RESUMO

This study investigated the degradation of ibuprofen (IBP), an activated persulfate (PS), when subjected to ultrasonic (US) irradiation and mechanical mixing (M). The effects of several critical factors were evaluated, including the effect of rpm on M, PS concentration, and initial pH, and that of temperature on IBP degradation kinetics and the PS activation mechanism. The resulting IBP oxidation rate constant was significantly higher at 400 rpm. As the PS load increased, the IBP oxidation rate constant increased. The value of the IBP reaction rate increased with decreasing pH; below pH 4.9, there was no significant difference in the IBP oxidation rate constant. The IBP oxidation activation energy when using the US/M-PS system was 18.84 kJ mol-1. In the US/M-PS system, PS activation was the primary effect of temperature at the interface during the explosion of cavitation bubbles. These encouraging results suggest that the US-PS/M process is a promising strategy for the treatment of IBP-based water pollutants.

8.
Ultrason Sonochem ; 66: 105106, 2020 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-32247235

RESUMO

Hydrodynamic cavitation (HC) and Fe(II) are advanced oxidation processes, in which pentachlorophenol (PCP) is treated by the redox method of activating persulfate (PS). The kinetics and mechanism of the HC and Fe(II) activation of PS were examined in aqueous solution using an electron spin resonance (ESR) spin trapping technique and radical trapping with pure compounds. The optimum ratio of Fe(II)/PS was 1:2, and the hydroxyl radical (HO) and sulfate radical (SO4-) generation rate were 5.56 mM h-1 and 8.62 µM h-1, respectively. The generation rate and Rct of HO and SO4- at pH 3 and 50 °C in the Fe(II)/PS/HC system are 7584.6 µM h-1, 0.013 and 24.02 µM h-1, 3.95, respectively. The number of radicals was reduced as the pH increased, and it increased with increasing temperature. The PCP reaction rate constants was 4.39 × 10-2 min-1 at pH 3 and 50 °C. The activation energy was 10.68 kJ mol-1. In addition, the mechanism of PCP treatment in the Fe(II)/PS/HC system was a redox reaction, and the HO-/SO4- contribution was 81.1 and 18.9%, respectively. In this study, we first examined PCP oxidation through HO and SO4- quantification using only the Fe(II)/PS/HC process. Furthermore, the results provide the foundation for activation of PS by HC and Fe(II), but also provide a data basis for similar organic treatments other than PCP.

9.
Nutrients ; 12(2)2020 Feb 05.
Artigo em Inglês | MEDLINE | ID: mdl-32033291

RESUMO

Atopic dermatitis (AD) is a chronic inflammatory skin disease caused mainly by immune dysregulation. This study explored the anti-inflammatory and immunomodulatory effects of the Centella asiatica ethanol extract (CA) on an AD-like dermal disorder. Treatment with CA inhibited the expression of interleukin-6 (IL-6) and tumor necrosis factor-α (TNF-α) in a dose-dependent manner in inflammatory stimulated HaCaT cells by interferon-γ (IFN-γ) and TNF-α-triggered inflammation. Eight-week-old BALB/c mice treated with 2,4-dinitrochlorobenzene (DNCB) were used as a mouse model of AD. In AD induce model, we had two types treatment of CA; skin local administration (80 µg/cm2, AD+CA-80) and oral administration (200 mg/kg/d, AD+CA-200). Interestingly, the CA-treated groups exhibited considerably decreased mast cell infiltration in the ear tissue. In addition, the expression of IL-6 in mast cells, as well as the expression of various pathogenic cytokines, such as TNF-α, IL-4, IL-5, IL-6, IL-10, IL-17, iNOS, COX-2, and CXCL9, was reduced in both AD+CA-80 and AD+CA-200 groups. Collectively, our data demonstrate the pharmacological role and signaling mechanism of CA in the regulation of allergic inflammation of the skin, which supports our hypothesis that CA could potentially be developed as a therapeutic agent for AD.


Assuntos
Anti-Inflamatórios/farmacologia , Dermatite Atópica/tratamento farmacológico , Imunomodulação/efeitos dos fármacos , Triterpenos/farmacologia , Administração Oral , Administração Tópica , Animais , Citocinas/sangue , Dermatite Atópica/induzido quimicamente , Dinitroclorobenzeno , Modelos Animais de Doenças , Inflamação , Mastócitos , Camundongos , Camundongos Endogâmicos BALB C , Pele/efeitos dos fármacos
10.
Chemosphere ; 238: 124559, 2020 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-31446279

RESUMO

The adsorption of bisphenol-A (BPA) on ordered mesoporous carbon (CMK-3) and modified CMK-3 (MCMK-3) for decontamination of aqueous medium was investigated. The CMK-3 and MCMK-3 materials had uniform pore sizes of 3.60 and 3.70 nm and high Brunauer-Emmett-Teller (BET) surface areas of 751 and 564 m2 g-1, respectively. The maximum adsorption capacities of CMK-3 and MCMK-3 were 178.57 (0.24 mg m-2) and 238.01 (0.42 mg m-2) mg g-1, respectively at 298 K (pH 6.4). The difference in the adsorption capacities is attributed to the specific surface area and hydrophobicity of the adsorbents. The adsorption of BPA on CMK-3 and MCMK-3 may be influenced by π-π bonding and hydrophobic and electrostatic interactions, and the excellent adsorption capacity of MCMK-3 is attributed to its unique sp2-hybridized single-atom-layer structure. The kinetics and isotherm data were described by the pseudo-second order kinetic model and the Langmuir isotherm, respectively. This difference in the adsorption kinetics of CMK-3 and MCMK-3 is caused by the increase in the pore diameter of the latter. Further, CMK-3 and MCMK-3, with an open geometry consisting of interlinked nanorods, allow for faster intraparticle diffusion. Overall, CMK-3 and MCMK-3 could be promising adsorbents for the removal of chemicals containing benzene rings from wastewater.


Assuntos
Compostos Benzidrílicos/isolamento & purificação , Carbono/química , Fenóis/isolamento & purificação , Águas Residuárias/análise , Poluentes Químicos da Água/isolamento & purificação , Purificação da Água/métodos , Adsorção , Compostos Benzidrílicos/metabolismo , Cinética , Fenóis/metabolismo , Águas Residuárias/química , Poluentes Químicos da Água/metabolismo
11.
Ultrason Sonochem ; 52: 326-335, 2019 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-30660376

RESUMO

A cost-effective method for treating oxalic acid (OA) during rare-earth extraction was developed using hydrodynamic cavitation (HC), ozone (O3), and persulfate (PS) (HC@PS@O3 process). The results showed that the optimal inlet pressure during HC was 5.10 kg cm-2 with an orifice plate diameter of 2 mm. Moreover, HC was shown to activate PS, providing an alternative activation method to base or heat as an ultrasound activation method for chemical oxidation. O3 was also shown to activate PS. For OA oxidation using the HC@PS@O3 process, the optimum pH was 3 and the reaction rate increased with increasing temperature. Further, the activation energy was 36.69 kJ mol-1. The mechanisms unveiled in this study will allow optimization of the HC@PS@O3 process as a chemical oxidation technology. The kinetic investigation and economic evaluation of the HC@PS@O3 process can be used as the basis for real wastewater treatment processes in the future.

12.
J Hazard Mater ; 344: 1116-1125, 2018 02 15.
Artigo em Inglês | MEDLINE | ID: mdl-30216971

RESUMO

Degradation of polychlorinated dibenzo-p-dioxins and dibenzofurans (PCDD/Fs) in real-field soil was conducted using an integrated photocatalysis-solvent migration system of BiVO4/Bi2O3 and n-hexane. The photocatalyst BiVO4/Bi2O3 was synthesized, and its performance was found to be affected by the BiVO4 content, with 20wt% BiVO4 showing the best performance owing to its p-n heterojunction being well formed. Migration was affected by the amount of n-hexane, with 15% n-hexane giving the most effective transportation of PCDD/Fs. 37.2% of 17 PCDD/Fs was removed in 60h by the integrated photocatalysis-solvent migration system, although the reaction zone covered 8.5% of the volume of the soil. The result showed that migration via n-hexane fulfilled the aim of carrying contaminants from inside of the soil to the surface. Electron-scavenging experiments with BiVO4/Bi2O3 showed an 18.4% of performance in removal compared to no-scavenging condition, which showed that the main reactions driving BiVO4/Bi2O3 visible-light photocatalysis for aryl-chloride were found to be reduction-based. Owing to the hindering effect of Cl atoms, degradation by hydroxyl radical could proceed after initial dechlorination. This study establishes the applicability of integrated photocatalysis-solvent migration systems in real-field settings, and is the first report of a visible-light photocatalyst, BiVO4/Bi2O3, for the degradation of PCDD/Fs in soil.

13.
Ultrason Sonochem ; 43: 193-200, 2018 May.
Artigo em Inglês | MEDLINE | ID: mdl-29555275

RESUMO

In order to enhance sonocatalytic oxidation of a recalcitrant organic pollutant, rhodamine B (RhB), it is necessary to study the fundamental aspects of sonocatalysis. In this study, TiO2-incorporated nano-structured carbon (i.e., carbon nanotubes (CNTs) or graphene (GR)) composites were synthesized by coating TiO2 on CNTs or GR of different mass percentages (0.5, 1, 5, and 10 wt%) by a facile hydrothermal method. The sonocatalytic degradation rates of RhB were examined for the effect of ultrasound (US) frequency and calcination temperature by using the prepared TiO2-NSC composites. Since US frequency affected the sonoluminescence (SL) intensities, it was proposed that there exists a correlation between the surface area or band-gap of the sonocatalysts and the degradation kinetic constants of RhB. In addition, the reusability of TiO2-GR composites was also investigated. Overall, the performance of TiO2-GRs prepared by the hydrothermal method was better than that of calcined TiO2-CNTs. Among TiO2-GRs, 5% GR incorporated media (TiO2-GR-5) showed the best performance. Interestingly, the kinetic constants of sonocatalysts prepared under hydrothermal conditions had a negative linear relationship with the band-gap energy for the corresponding media. Furthermore, the strongest SL intensity and highest degradation rates of RhB for both carbonaceous composites were observed at 500 kHz. The kinetic constants of calcined media decreased linearly as the specific area of the media decreased, while the band-gap energy could not be correlated with the kinetic constants. The GR combined TiO2 composite might be a good sonocatalyst in wastewater treatment using ultrasound-based oxidation because of its high stability.

14.
Biosci Biotechnol Biochem ; 80(1): 80-8, 2016.
Artigo em Inglês | MEDLINE | ID: mdl-26613402

RESUMO

Skeletal muscle atrophy can be defined as a decrease of muscle volume caused by injury or lack of use. This condition is associated with reactive oxygen species (ROS), resulting in various muscular disorders. We acquired 2D and 3D images using micro-computed tomography in gastrocnemius and soleus muscles of sciatic-denervated mice. We confirmed that sciatic denervation-small animal model reduced muscle volume. However, the intraperitoneal injection of Oenothera odorata root extract (EVP) delayed muscle atrophy compared to a control group. We also investigated the mechanism of muscle atrophy's relationship with ROS. EVP suppressed expression of SOD1, and increased expression of HSP70, in both H2O2-treated C2C12 myoblasts and sciatic-denervated mice. Moreover, EVP regulated apoptotic signals, including caspase-3, Bax, Bcl-2, and ceramide. These results indicate that EVP has a positive effect on reducing the effect of ROS on muscle atrophy.


Assuntos
Músculo Esquelético/efeitos dos fármacos , Atrofia Muscular/tratamento farmacológico , Oenothera/química , Extratos Vegetais/farmacologia , Espécies Reativas de Oxigênio/antagonistas & inibidores , Transdução de Sinais/efeitos dos fármacos , Animais , Apoptose/efeitos dos fármacos , Caspase 3/genética , Caspase 3/metabolismo , Linhagem Celular , Ceramidas/metabolismo , Denervação/efeitos adversos , Modelos Animais de Doenças , Regulação da Expressão Gênica , Proteínas de Choque Térmico HSP70/agonistas , Proteínas de Choque Térmico HSP70/genética , Proteínas de Choque Térmico HSP70/metabolismo , Injeções Intraperitoneais , Masculino , Camundongos , Camundongos Endogâmicos C57BL , Músculo Esquelético/metabolismo , Músculo Esquelético/patologia , Atrofia Muscular/etiologia , Atrofia Muscular/metabolismo , Atrofia Muscular/patologia , Mioblastos/citologia , Mioblastos/efeitos dos fármacos , Mioblastos/metabolismo , Proteínas Proto-Oncogênicas c-bcl-2/genética , Proteínas Proto-Oncogênicas c-bcl-2/metabolismo , Espécies Reativas de Oxigênio/metabolismo , Nervo Isquiático/cirurgia , Superóxido Dismutase/antagonistas & inibidores , Superóxido Dismutase/genética , Superóxido Dismutase/metabolismo , Superóxido Dismutase-1 , Proteína X Associada a bcl-2/genética , Proteína X Associada a bcl-2/metabolismo
15.
Artigo em Inglês | MEDLINE | ID: mdl-25945103

RESUMO

Muscle atrophy, a reduction of muscle mass, strength, and volume, results from reduced muscle use and plays a key role in various muscular diseases. In the microgravity environment of space especially, muscle atrophy is induced by muscle inactivity. Exposure to microgravity induces muscle atrophy through several biological effects, including associations with reactive oxygen species (ROS). This study used 3D-clinostat to investigate muscle atrophy caused by oxidative stress in vitro, and sciatic denervation was used to investigate muscle atrophy in vivo. We assessed the effect of Oenothera odorata root extract (EVP) on muscle atrophy. EVP helped recover cell viability in C2C12 myoblasts exposed to microgravity for 24 h and delayed muscle atrophy in sciatic denervated mice. However, the expressions of HSP70, SOD1, and ceramide in microgravity-exposed C2C12 myoblasts and in sciatic denervated mice were either decreased or completely inhibited. These results suggested that EVP can be expected to have a positive effect on muscle atrophy by disuse and microgravity. In addition, EVP helped characterize the antioxidant function in muscle atrophy.

16.
Biosci Biotechnol Biochem ; 78(9): 1568-71, 2014.
Artigo em Inglês | MEDLINE | ID: mdl-25209505

RESUMO

Nodakenin, derived from the roots of Angelica gigas Nakai, is an important natural resource and medicinal material with anti-allergic and anti- inflammatory activities. We have previously shown that nodakenin inhibits IgE/Ag-induced degranulation in mast cells. In this study, we investigated the inhibitory effect of nodakenin on 2,4-dinitrochlorobenzene (DNCB)-induced atopic dermatitis (AD)- like skin lesions in ICR mice. Scratching behavior, skin severity score, blood IgE level, and skin thickness were improved in DNCB-induced AD-like ICR mice. Our results showed that nodakenin suppressed the increase of AD-like skin lesions in ICR mice. These results suggest that nodakenin may be a potential therapeutic resource for AD as well as an adjunctive agent to control itching associated with AD.


Assuntos
Cumarínicos/administração & dosagem , Dermatite Atópica/tratamento farmacológico , Glucosídeos/administração & dosagem , Prurido/tratamento farmacológico , Pele/efeitos dos fármacos , Angelica/química , Animais , Degranulação Celular/efeitos dos fármacos , Dermatite Atópica/induzido quimicamente , Dermatite Atópica/metabolismo , Humanos , Camundongos , Nitrobenzoatos/toxicidade , Prurido/induzido quimicamente , Prurido/patologia , Pele/patologia
17.
Biosci Biotechnol Biochem ; 77(10): 2134-6, 2013.
Artigo em Inglês | MEDLINE | ID: mdl-24096648

RESUMO

We investigated the effects of a Eupatorium chinense var. simplicifolium (EUC) root extract on muscle disorders and explored the underlying mechanism for oxidative stress-induced C(2)C(12) myoblast damage. An EUC pre-treatment reduced the decreased cell viability after an H2O2 treatment. The heat shock protein (HSP) 70 level increased, and the phosphorylation of Jun amino-terminal kinases (JNKs) decreased in the EUC-pre-treated C(2)C(12) myoblasts. The results of the present study demonstrate the potential benefit of a herbal medicine in treating oxidative stress-related muscle disorders.


Assuntos
Apoptose/efeitos dos fármacos , Eupatorium/química , Mioblastos/citologia , Mioblastos/efeitos dos fármacos , Extratos Vegetais/farmacologia , Raízes de Plantas/química , Linhagem Celular , Mioblastos/metabolismo , Estresse Oxidativo/efeitos dos fármacos
18.
Biol Pharm Bull ; 36(8): 1375-9, 2013.
Artigo em Inglês | MEDLINE | ID: mdl-23902981

RESUMO

Atopic dermatitis (AD) is a chronic, allergic, and inflammatory skin disease associated with eczema and dermatitis symptoms. Our previous studies have reported that eriodictyol extract inhibits immunoglobulin E (IgE)/Ag-induced type I hypersensitivity by suppressing the activation of proinflammatory cytokines, such as interleukin-4 (IL-4), and the expression of ceramide kinase. In this study, we investigated the inhibitory effect of eriodictyol on 2,4-dinitrochlorobenzene (DNCB)-induced AD-like skin lesions in ICR mice. Treatment with 2 mg/mL eriodictyol for DNCB-induced AD-like skin lesions in ICR mice improved scratching behavior and skin severity score. Histological analysis demonstrated that thickening of the skin lesions were significantly reduced in the eriodictyol-treated group. Also, eriodictyol suppressed the DNCB-mediated elevation of IgE serum levels. These results suggest that eriodictyol may be a potential therapeutic resource for AD and an adjunctive agent to control itchiness in AD.


Assuntos
Antialérgicos/uso terapêutico , Anti-Inflamatórios/uso terapêutico , Dermatite Atópica/tratamento farmacológico , Flavanonas/uso terapêutico , Prurido/tratamento farmacológico , Animais , Comportamento Animal/efeitos dos fármacos , Dermatite Atópica/sangue , Dermatite Atópica/induzido quimicamente , Dermatite Atópica/patologia , Dinitroclorobenzeno , Imunoglobulina E/sangue , Masculino , Camundongos , Camundongos Endogâmicos ICR , Prurido/sangue , Prurido/induzido quimicamente , Prurido/patologia , Pele/patologia
19.
Biosci Biotechnol Biochem ; 77(2): 375-7, 2013.
Artigo em Inglês | MEDLINE | ID: mdl-23391909

RESUMO

Cell injury associated with reactive oxygen species (ROS) has been reported in various muscular disorders. We found that a Cichorium intybus (Cii) extract reduced H(2)O(2)-induced viability loss in C2C12 myoblasts, inhibited oxidative stress-induced apoptosis and increased intracellular heat shock protein 70 (Hsp 70) expression. Cii also inhibited the level of intracellular ceramide. These results indicate that Cii may prevent skeletal muscle atrophy by inducing the expression of Hsp 70 and inhibiting the level of ceramide.


Assuntos
Antioxidantes/farmacologia , Chicória/química , Mioblastos/efeitos dos fármacos , Estresse Oxidativo/efeitos dos fármacos , Extratos Vegetais/farmacologia , Raízes de Plantas/química , Acetilcisteína/farmacologia , Animais , Antioxidantes/isolamento & purificação , Apoptose/efeitos dos fármacos , Linhagem Celular , Sobrevivência Celular/efeitos dos fármacos , Ceramidas/antagonistas & inibidores , Ceramidas/biossíntese , Expressão Gênica/efeitos dos fármacos , Proteínas de Choque Térmico HSP70/genética , Proteínas de Choque Térmico HSP70/metabolismo , Peróxido de Hidrogênio/farmacologia , Camundongos , Mioblastos/citologia , Mioblastos/metabolismo , Extratos Vegetais/isolamento & purificação , Espécies Reativas de Oxigênio/antagonistas & inibidores , Espécies Reativas de Oxigênio/metabolismo
SELEÇÃO DE REFERÊNCIAS
DETALHE DA PESQUISA
...