Your browser doesn't support javascript.
loading
Mostrar: 20 | 50 | 100
Resultados 1 - 2 de 2
Filtrar
Mais filtros










Base de dados
Intervalo de ano de publicação
1.
Int J Clin Exp Pathol ; 7(11): 7451-9, 2014.
Artigo em Inglês | MEDLINE | ID: mdl-25550780

RESUMO

OBJECTIVE: This study aims to explore the function of Integrin-ß/FAK in the mechanical signal transduction and the connection with downstream ERK signal pathways. METHODS: Human osteosarcoma MG63 cell lines were used in this study. The effects of mechanical strain on the Integrin-ß1 expression, FAK and ERK signal pathway in Human osteosarcoma MG63 cells were detected using RT-PCR and Western-blotting methods. The localization of FAK in Human osteosarcoma MG63 cells were determined using immunofluorescent method. The interaction between Integrin-ß1 and FAK were detected by using co-immunoprecipitation method. RESULTS: The expression of Integrin-ß1 shows a notable bimodel distribution, mechanical strain stimulation can promote Integrin-ß1 expression and the phosphorylation of FAK and ERK, mechanical strain activated FAK and ERK mediated by Integrin-ß1. CONCLUSION: Integrin-ß1 may play an important role in osteoblast proliferation differentiation process, it might feel external strain stimulation through ECM composition and makes FAK phosphated through the interaction with FAK, thus causing a series of activation of signal molecules. Finally it reduces MAPK (ERK) activation and cellular responses to finish mechanical signal transduction.


Assuntos
Quinase 1 de Adesão Focal/metabolismo , Integrina beta1/metabolismo , Sistema de Sinalização das MAP Quinases , Diferenciação Celular , Linhagem Celular Tumoral , Proliferação de Células , Quinase 1 de Adesão Focal/genética , Humanos , Integrina beta1/genética , Fosforilação
2.
Cell Physiol Biochem ; 28(2): 297-304, 2011.
Artigo em Inglês | MEDLINE | ID: mdl-21865737

RESUMO

To explore the possible role for connective tissue growth factor (CTGF) during tooth movement, we evaluated CTGF gene and protein expression in MG-63 cells subjected to cyclic stretch. Cyclic stretch caused a time-dependent increase in CTGF mRNA and protein levels.Inhibition of p38 MAP kinase or ERK activation did not affect cyclic stretch-induced CTGF expression. Specific inhibitors of PI3K suppressed stretch -induced CTGF expression in a time-dependent manner. cyclic stretch activated JNK and ERK, but not p38 MAP kinase in osteoblast-like cells. PI3K inhibitors suppressed cyclic stretch-induced JNK, but not p38 MAP kinase activation. Finally, SP600125, a Specific Inhibitor of JNK, suppressed stretch -induced CTGF Expression. These results suggest that stretch-induced CTGF expression is mediated through the PI3K-JNK -dependent pathway, not by p38 MAP kinase and ERK pathways.


Assuntos
Fator de Crescimento do Tecido Conjuntivo/metabolismo , Osteoblastos/metabolismo , Transdução de Sinais , Estresse Mecânico , Antracenos/farmacologia , Linhagem Celular , Cromonas/farmacologia , Fator de Crescimento do Tecido Conjuntivo/genética , MAP Quinases Reguladas por Sinal Extracelular/antagonistas & inibidores , MAP Quinases Reguladas por Sinal Extracelular/metabolismo , Flavonoides/farmacologia , Humanos , Proteínas Quinases JNK Ativadas por Mitógeno/antagonistas & inibidores , Proteínas Quinases JNK Ativadas por Mitógeno/metabolismo , Morfolinas/farmacologia , Osteoblastos/efeitos dos fármacos , Fosfatidilinositol 3-Quinases/metabolismo , Inibidores de Fosfoinositídeo-3 Quinase , Proteínas Quinases p38 Ativadas por Mitógeno/metabolismo
SELEÇÃO DE REFERÊNCIAS
DETALHE DA PESQUISA
...