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1.
Sci Adv ; 7(7)2021 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-33568480

RESUMO

Evidence that offspring traits can be shaped by parental life experiences in an epigenetically inherited manner paves a way for understanding the etiology of depression. Here, we show that F1 offspring born to F0 males of depression-like model are susceptible to depression-like symptoms at the molecular, neuronal, and behavioral levels. Sperm small RNAs, and microRNAs (miRNAs) in particular, exhibit distinct expression profiles in F0 males of depression-like model and recapitulate paternal depressive-like phenotypes in F1 offspring. Neutralization of the abnormal miRNAs in zygotes by antisense strands rescues the acquired depressive-like phenotypes in F1 offspring born to F0 males of depression-like model. Mechanistically, sperm miRNAs reshape early embryonic transcriptional profiles in the core neuronal circuits toward depression-like phenotypes. Overall, the findings reveal a causal role of sperm miRNAs in the inheritance of depression and provide insight into the mechanism underlying susceptibility to depression.

2.
Wei Sheng Wu Xue Bao ; 57(1): 33-42, 2017 Jan 04.
Artigo em Chinês | MEDLINE | ID: mdl-29746085

RESUMO

Objective: The objective of this research was to study plant cell wall degradation enzymes from Fusarium sp. Q7-31T. Methods: Strain was cultured in liquid medium with 1% (W/V) peptone as nitrogen source, 0.5% (W/V) oat straw as carbon source, 120 r/min shaking at 20 °C for 3 days. The endoglucanase Egn21 was purified by using Sephacry S-100 chromatography and DEAE-sepharose ion-exchange column chromatography. Then the enzymatic properties and MADIL-TOF-TOF identification were analyzed. Results: The molecular weight and isoelectric point (pI) of Egn21 was 44.25 kDa and 4.91, respectively. Egn21 had optimal activity with carboxymethyl cellulose at 40 °C and pH 6.0, stable at 45 °C and pH between 5.0 and 8.0, inhibited by Fe2+, Ca2+, K+, Na+, Mn2+ and inactivated by Hg2+, whereas Co2+, Zn2+ and Mg2+ had no effect. Conclusion: The enzymatic properties and MADIL-TOF-TOF results suggested that Egn21 belongs to GH5 family.


Assuntos
Celulase/química , Celulase/isolamento & purificação , Proteínas Fúngicas/química , Fusarium/enzimologia , Celulase/genética , Celulase/metabolismo , Cromatografia em Gel , Cromatografia por Troca Iônica , Estabilidade Enzimática , Proteínas Fúngicas/genética , Proteínas Fúngicas/metabolismo , Fusarium/química , Fusarium/genética , Concentração de Íons de Hidrogênio , Ponto Isoelétrico , Cinética , Espectrometria de Massas , Peso Molecular , Temperatura
3.
Carbohydr Polym ; 117: 1-10, 2015 Mar 06.
Artigo em Inglês | MEDLINE | ID: mdl-25498602

RESUMO

The purpose of this study was to prepare a kind of novel pH-response dietary fiber from chitosan-coated konjac glucomannan (KGM) powders (KGM/Chitosan or K/C powders) by a physical grind method. The K/C powders were selectively soluble in aqueous solutions of different pH. Meanwhile, the coated chitosan could largely decrease the viscosity of KGM in neutral condition, which is the main limitation for KGM application in food industry. Fourier-transform infrared (FTIR) spectroscopy, scanning electron microscopy (SEM), X-ray photoelectron spectroscopy (XPS), swelling ability and rheological measurements were utilized to characterize the performance of K/C powders. K/C powders exhibited much higher viscosity and swelling ability in acidic condition than in neutral condition. Therefore, this study will extend the application of KGM in food industry and in other pH-specific applications as well.


Assuntos
Quitosana/química , Fibras na Dieta , Mananas/química , Elasticidade , Concentração de Íons de Hidrogênio , Reologia , Propriedades de Superfície , Viscosidade
4.
Food Chem ; 158: 171-6, 2014 Sep 01.
Artigo em Inglês | MEDLINE | ID: mdl-24731328

RESUMO

A simple one-step purification process was provided to extract KGM from KF by phase separation. The results showed that appropriate temperature control was a key factor and the products were inodorous, colourless and of high purity at the optimal temperature 68 °C. In this purification, soluble sugar and starch of extracted KGM were nearly clearly reduced and up to 95%, 80% (T68) of protein and ash were removed, respectively as compared with KF. Odour and transparency were improved 4 ranks and 30%, respectively. Besides, the ηapp reached 42.30 Pa s and increased by 93.55% as compared, which could stay at a steady level for a week. Furthermore, morphology of extracted KGM displayed regular lamellar and wrinkling distribution for removed impurities. The temperature-controlled method not only enriches the knowledge of KGM purification but also has the potential to broaden the application of KGM.


Assuntos
Farinha/análise , Mananas/química , Reologia , Temperatura
5.
Colloids Surf B Biointerfaces ; 114: 60-6, 2014 Feb 01.
Artigo em Inglês | MEDLINE | ID: mdl-24161507

RESUMO

In this study, polyethylene terephthalate/polypropylene (PET/PP) films were treated via atmospheric pressure plasma, assembled with chitosan and various preservatives and applied for antimicrobial food packaging. Surface properties of these obtained films were studied by contact angle measurement, atomic force microscopy (ATM), X-ray photoelectron spectroscopy (XPS), Fourier transformed infrared spectroscopy (FT-IR) and dynamic laser scattering (DLS). The above results showed that the surface hydrophilicity and roughness of the films increased after the plasma treatment. Besides, chitosan and the preservatives were successfully assembled onto the surface of the films. In addition, the antimicrobial activities of the films against three kinds of microorganisms (Staphylococcus aureus, Bacillus subtilis and Escherichia coli) were investigated and the results indicated that the inhibition ratios against B. subtilis and E. coli reached almost 100% while the inhibition ratios against S. aureus were lower than 85%. Moreover, the accumulative release profiles of the antimicrobial substances migrating from the assembled films into the release solutions revealed that their release speed increased with the increment of temperature and acidity, but decreased with enhancing the ionic strength regulated by sodium chloride or with lowering the ionic mobility regulated by sucrose.


Assuntos
Anti-Infecciosos/farmacologia , Quitosana/química , Embalagem de Alimentos , Gases em Plasma/química , Polietilenotereftalatos/química , Polipropilenos/química , Bacillus subtilis/efeitos dos fármacos , Escherichia coli/efeitos dos fármacos , Testes de Sensibilidade Microbiana , Microscopia de Força Atômica , Espectroscopia Fotoeletrônica , Ácido Sórbico/farmacologia , Staphylococcus aureus/efeitos dos fármacos , Eletricidade Estática , Propriedades de Superfície , Água/química
6.
Int J Pharm ; 441(1-2): 721-7, 2013 Jan 30.
Artigo em Inglês | MEDLINE | ID: mdl-23089579

RESUMO

Lysozyme (Ly) and sodium carboxymethyl cellulose (CMC) were used to fabricate nanogels by a convenient method without using any chemical treatment except simple heating to achieve the denaturation temperature of Ly. The prepared nanogels were characterized by dynamic laser scattering (DLS), rheological analysis, transmission electron microscopy (TEM), field emission scanning electron microscope (FE-SEM) and X-ray photoelectron spectroscopy (XPS). The nanogels are of spherical shape with average hydrodynamic diameter of 241 nm and the swelling ratio of nanogels is about 5. Then 5-fluorouracil was used as a model drug to investigate the entrapment efficiency and release ability in nanogels. It turned out to be that the release in simulated gastric fluid (SGF) was more slowly compared with that in simulated intestinal fluid (SIF), which could protect the 5-Fu in stomach and ensure it released in intestines.


Assuntos
Antimetabólitos Antineoplásicos/administração & dosagem , Carboximetilcelulose Sódica/química , Fluoruracila/administração & dosagem , Muramidase/química , Preparações de Ação Retardada , Portadores de Fármacos/química , Sistemas de Liberação de Medicamentos , Suco Gástrico/metabolismo , Géis , Secreções Intestinais/metabolismo , Lasers , Microscopia Eletrônica de Varredura , Microscopia Eletrônica de Transmissão , Nanopartículas , Tamanho da Partícula , Espectroscopia Fotoeletrônica , Reologia , Espalhamento de Radiação , Temperatura
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