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Zhongguo Zhong Yao Za Zhi ; 44(20): 4448-4453, 2019 Oct.
Artigo em Chinês | MEDLINE | ID: mdl-31872631


Musk,with unique and intense perfume,was a kind of deep brown precious medicinal material in traditional Chinese medicine. However,the immature musk in musk pot was white and stench. Given the fact that bacterial diversity generated odorous metabolites in animal hosts,in this study,musk samples at three different mature stages,including MJ( the end of June),MA( the end of August) and MO( the end of October) were harvested from three male forest musk deer,and then next-generation sequencing was used to intensively survey the bacterial communities in musk harvested at different mature stages. RESULTS: indicated that the average OTUs per sample at the end of June,August and October were 47 116. 00 ± 1 567. 24( SE),52 009. 00 ± 8 958. 75( SE) and50 004. 67±4 135. 57( SE),respectively. Feature of the musk 16 S rRNA gene showed a total of 418 genera belonging to 52 phyla were observed in all samples. The main microbiota was bacteria,which accounted for 98. 82%,99. 95% and 99. 58% in MJ,MA and MO,respectively. At phylum level,Firmicutes was the most abundant bacterial of MA( 32. 75%) and MO( 39. 19%). While,the major bacterial in MJ was Proteobacteria( 49. 14%). PICRUSt analysis revealed the functions of bacterial in MJ were mainly involved in secretion,while bacterial functions of MA and MO were mainly involved in amino acid or other substance metabolism,which was in accord with the musk secretion physiological process of forest musk deer. This is the first study involved in the bacterial diversity in musk of forest musk deer across the maturation process,while may provide a new insight into the musk generation mechanism.

Cervos/microbiologia , Ácidos Graxos Monoinsaturados , Animais , Florestas , Sequenciamento de Nucleotídeos em Larga Escala , Masculino
Zhong Yao Cai ; 38(6): 1119-22, 2015 Jun.
Artigo em Chinês | MEDLINE | ID: mdl-26762048


OBJECTIVE: To confirm the current major diseases and corresponding pathogens of Codonopsis tangshen in Chongqing. METHODS: The main cultivation regions of Codonopsis tangshen in Chongqing were systematically investigated, and the pathogens of the obtained specimens were isolated and identified. RESULTS: Totally, five fungal diseases in Codonopsis tangshen were identified, including rust disease (Puccinia campanumoeae Pat.), root rot (Fusarium oxysporum Schl.), violet root rot (Helicobasidium mompa Tanaka), powdery mildew (Sphaerotheca codonopsis (Golov.) Z. Y. Zhao), and blight (Septoria codonopsidis Ziling). Currently, the diseases with the serious damage on Codonopsis tangshen included rust disease, root rot and violet root rot. CONCLUSION: Rust disease, the severest disease,whose incidence reaches 100%, is an urgent problem waiting to be solved effectively in Codonopsis tangshen cultivation.

Codonopsis/microbiologia , Doenças das Plantas/microbiologia , Ascomicetos , Basidiomycota , China , Fusarium , Raízes de Plantas/microbiologia