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1.
Echocardiography ; 36(7): 1253-1262, 2019 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-31287587

RESUMO

OBJECTIVE: B-line imaging by lung ultrasound (LUS) is a new tool for evaluating subclinical pulmonary congestion. The aim of this study was to explore the prognostic value of B-line number at admission in predicting symptomatic heart failure (HF) during hospitalization in acute anterior wall STEMI patients. METHODS: This was a prospective cohort study which consecutively enrolled 96 anterior wall STEMI patients without dyspnea at admission. Pulmonary auscultation, NT-proBNP test, LUS, and echocardiography were performed within 5 hours after primary PCI. Rale occurrence, plasma NT-proBNP levels, B-line number, LVEF, E/e' were recorded, and their predictive value for HF in-hospital was analyzed. RESULTS: A total of 19 patients developed symptomatic HF. Median B-line number, NT-proBNP levels, and E/e' in the HF group were higher than those of the nonheart-failure (NHF) group (P < 0.001) while LVEF was lower (P = 0.002). There was no statistical difference in rale occurrence between the two groups. Multivariate logistic regression demonstrated that B-lines, E/e', and NT-proBNP independently predicted HF during hospitalization. According to the area under the ROC curve, the strongest predictor is B-lines (0.972), followed by NT-proBNP (0.936) and E/e' (0.928), and combining the three indicators was better than any single parameter (P = 0.048). B-line cutoff ≥18 could well predict HF event with specificity and sensitivity of 94.7% and 94.8%, respectively. CONCLUSION: Subclinical pulmonary congestion reflected by B-lines can independently predict symptomatic HF during hospitalization in patients with anterior wall STEMI, LUS will act as a complementary tool for evaluating cardiac function.

2.
Microbiol Immunol ; 63(8): 303-315, 2019 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-31218724

RESUMO

We investigated the correlation between the beneficial effect of Lactobacillus acidophilus on gut microbiota composition, metabolic activities, and reducing cow's milk protein allergy. Mice sensitized with ß-lactoglobulin (ß-Lg) were treated with different doses of L. acidophilus KLDS 1.0738 for 4 weeks, starting 1 week before allergen induction. The results showed that intake of L. acidophilus significantly suppressed the hypersensitivity responses, together with increased fecal microbiota diversity and short-chain fatty acids (SCFAs) concentration (including propionate, butyrate, isobutyrate, and isovalerate) when compared with the allergic group. Moreover, treatment with L. acidophilus induced the expression of SCFAs receptors, G-protein-coupled receptors 41 (GPR41) and 43 (GPR43), in the spleen and colon of the allergic mice. Further analysis revealed that the GPR41 and GPR43 messenger RNA expression both positively correlated with the serum concentrations of transforming growth factor-ß and IFN-γ (p < .05), but negatively with the serum concentrations of IL-17, IL-4, and IL-6 in the L. acidophilus-treated group compared with the allergic group (p < .05). These results suggested that L. acidophilus protected against the development of allergic inflammation by improving the intestinal flora, as well as upregulating SCFAs and their receptors GPR41/43.


Assuntos
Ácidos Graxos Voláteis/metabolismo , Intestinos/microbiologia , Lactobacillus acidophilus/fisiologia , Lactoglobulinas/efeitos adversos , Receptores Acoplados a Proteínas-G/metabolismo , Animais , Butiratos/metabolismo , Colo/metabolismo , Modelos Animais de Doenças , Fezes/microbiologia , Feminino , Microbioma Gastrointestinal/fisiologia , Interferon gama/metabolismo , Interleucina-17/sangue , Interleucina-4/sangue , Interleucina-6/sangue , Isobutiratos/metabolismo , Camundongos , Camundongos Endogâmicos BALB C , Hipersensibilidade a Leite/terapia , Proteínas do Leite , Ácidos Pentanoicos/metabolismo , Propionatos/metabolismo , RNA Mensageiro/metabolismo , Baço/metabolismo , Fator de Crescimento Transformador beta/metabolismo
3.
Biosci Biotechnol Biochem ; 82(11): 1955-1963, 2018 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-30156972

RESUMO

This study aims to investigate the correlation between the ability of L. acidophilus to modulate miRNA expression and prevent Th17-dominated ß-lactoglobulin (ß-Lg) allergy. In vitro immunomodulation was evaluated by measuring splenocyte proliferation, Th17-related immune response and miRNA expression in ß-Lg-sensitized splenocytes cultured with live L. acidophilus. Next, the allergic mouse model was used to evaluate anti-allergy capability of lactobacilli. The ß-Lg challenge led to induction of up-regulation of miR-146a, miR-155, miR-21 and miR-9 expression in both in vivo and in vitro, along with increased Th17-related cytokine levels and mRNA expression of RORγt and IL-17. However, treatment of live L. acidophilus significantly suppressed hypersensitivity responses and Th17 cell differentiation. Moreover, administration of live L. acidophilus reduced expression of four miRNAs, especially miR-146a and miR-155. In addition, the decreased expression of the miRNAs in the spleen of the L. acidophilus-treated group was closely associated with decrease of IL-17 and RORγt mRNA expression.

4.
Int J Cardiovasc Imaging ; 34(12): 1895-1904, 2018 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-30062538

RESUMO

Right ventricular (RV) function is a significantly important factor in the determination of the prognosis of chronic thromboembolic pulmonary hypertension (CTEPH) patients. Speckle-tracking echocardiography (STE) is an angle-independent new technique for quantifying myocardial deformation that is capable of providing data on multiple parameters including longitudinal and transverse information of the myocardium. In the present study, we aimed to study the advantages of STE-derived parameters in identifying RV dysfunction in CTEPH patients. Sixty CTEPH patients (mean age: 55 years ± 13 years; 25 males) and 30 normal controls (mean age: 54 years ± 14 years; 14 males) were enrolled in this study. RV free wall (RVFW) systolic peak longitudinal strain (LS) including the basal, mid-, and apical-segments and the basal longitudinal and transverse displacement (basal-DL and basal-DT) were measured by STE. Global LS (GLS) of the RV was calculated by averaging the LS value of the 3 segments of RVFW. Clinical data of CTEPH patients were collected. CTEPH patients were divided into 2 subgroups according to the World Health Organization function classification. Clinical right heart failure (RHF) was defined as the presence of symptoms of heart failure and signs of systemic circulation congestion during hospitalization. The apical segment LS of the RVFW was lower than that in the basal and mid-segments in the control group (P < 0.001), but no significant difference was found among the 3 segments of LS in the CTEPH group (P = 0.263). When we used the cutoff value recommended by the American Society of Echocardiography guidelines to identify abnormal RV function, 30 CTEPH patients (50%) by tricuspid annular plane systolic excursion (TAPSE), 42 patients (70%) by fractional area change (FAC), 20 patients (33.33%) by RV index of myocardial performance (RVIMP), and 46 patients (77%) patients by GLS were determined to have abnormal RV function, respectively. Among multiple RV function indicators, TAPSE, FAC, GLS, basal-DL, and N-terminal pronatriuretic B-type natriuretic peptide showed significant differences between CTEPH patients with mild (WHO II) and severe symptoms (WHO III/IV) (all P < 0.001), while RVIMP and basal-DT showed no significant difference (P = 0.188 and P = 0.394, respectively). Pearson correlation analysis showed that GLS has no correlation with sPAP as evaluated by echocardiography in CTEPH patients (r = - 0.079, P = 0.574), and a weak to moderate correlation with RA area (r = 0.488, P = 0.000), the RV diameter (r = 0.429, P = 0.001), and the RVFW thickness (r = 0.344, P = 0.009). On receiver operating characteristic analysis, GLS has the largest area under the curve to identify RHF when the cutoff value was - 13.45%, the sensitivity was 78.2%, and the specificity was 84.6%, separately. Our study demonstrated that the depression of regional LS of RVFW is more pronounced in the basal and middle segments in CTEPH patients. Also, the longitudinal movement is much more important than the transverse movement when evaluating RV systolic function. As compared with conventional parameters, RVFW GLS showed more sensitivity to identify abnormal RV function and had the largest AUC for identifying RHF. Additionally, GLS showed no correlation with sPAP and a weak correlation with right heart morphological parameters in our CTEPH cohort.

5.
Iran J Allergy Asthma Immunol ; 16(2): 147-158, 2017 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-28601055

RESUMO

Our previous study reported that Lactobacillus acidophilus(L.acidophilus)  key laboratory of dairy science  (KLDS) 1.0738 had an effective impact on inhibiting ß-lactoglobulin (ß-lg) allergy. This study further investigated the anti-allergic activity of peptidoglycan (PGN) isolated from KLDS 1.0738. This study aimed to assess whether toll-like receptor 2 (TLR2)/NF-kappaB (NF-κB) signaling activated by PGN was responsible for reducing allergic inflammation.  Our data showed that administration of  L. acidophilus PGN inhibited IgE production and improved the Treg/Th17 balance toward a Treg response in a mouse model of ß-lg allergy. In addition, treating different doses L. acidophilus PGN to sensitized mice significantly increased TLR2 levels, along with enhancing NF-κB expression, especially in medium and high concentration (p<0.05). Further analysis revealed that the mRNA expression of TLR2 and NF-κB were positively correlated with the Foxp3 mRNA expression (p<0.05), but were negatively correlated with the RORγt mRNA expression in L. acidophilus PGN-treated group compared to allergy group (p<0.05). This study suggests PGN was similar to probiotics in preventing ß-lg allergy through regulating Treg/Th17 imbalance, and activation of TLR2/NF-κB signaling may be involved in this process.

6.
Chin J Integr Med ; 2017 May 11.
Artigo em Inglês | MEDLINE | ID: mdl-28497390

RESUMO

OBJECTIVES: To evaluate whether garlicin post-conditioning can attenuate myocardial ischemiareperfusion injury in a catheter-based porcine model of acute myocardial infarction (AMI) by affecting adhesion molecules integrin ß1/CD29 and platelet endothelial cell adhesion molecule-1 (PECAM-1/CD31). METHODS: Twenty-two swine were devided into 3 groups: 6 in a sham-operation group, and 8 each in the model and garlicin groups. AMI porcine model was established in the model and garlicin groups. The distal parts of the left anterior descending coronary artery in the animals of the model and garlicin groups were occluded by dilated balloon for 2 h, followed by reperfusion for 3 h. Garlicin (1.88 mg/kg) was injected over a period of 1 h, beginning just before reperfusion, in the garlicin group. Real-time polymerase chain reaction, immunohistochemistry and Western blot were carried out to detect mRNA and protein expressions of CD29 and CD31 3 h after reperfusion. RESULTS: Hematoxylin-eosin staining showed a better myocardial structure in the garlicin group after reperfusion. Compared to the model group, garlicin inhibited both the mRNA and protein expression of CD29 and CD31 in reperfusion area and no-reflflow area (P<0.05 respectively). CONCLUSIONS: Garlicin post-conditioning induced cardio-protection against myocardial ischemia-reperfusion injury in this catheter-based porcine model of AMI. The cardio-protective effect of garlicin is possibly owing to suppression of production of CD29 and CD31, by inhibition of the mRNA expression of CD29 and CD31.

7.
J Food Sci ; 81(7): M1726-34, 2016 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-27228279

RESUMO

Microencapsulation is an effective way to improve the survival of probiotics in simulated gastrointestinal (GI) conditions and yoghurt. In this study, microencapsulation of Lactobacillus rhamnosus GG (LGG) was prepared by first cross-linking of soy protein isolate (SPI) using transglutaminase (TGase), followed by embedding the bacteria in cross-linked SPI, and then freeze-drying. The survival of microencapsulated LGG was evaluated in simulated GI conditions and yoghurt. The results showed that a high microencapsulation yield of 67.4% was obtained. The diameter of the microencapsulated LGG was in the range of 52.83 to 275.16 µm. Water activity did not differ between free and microencapsulated LGG after freeze-drying. The survival of microencapsulated LGG under simulated gastric juice (pH 2.5 and 3.6), intestinal juice (0.3% and 2% bile salt) and storage at 4 °C were significantly higher than that of free cells. The survival of LGG in TGase cross-linked SPI microcapsules was also improved to 14.5 ± 0.5% during storage in yoghurt. The microencapsulation of probiotics by TGase-treated SPI can be a suitable alternative to polysaccharide gelation technologies.


Assuntos
Composição de Medicamentos/métodos , Trato Gastrointestinal/química , Lactobacillus rhamnosus/crescimento & desenvolvimento , Probióticos , Proteínas de Soja/metabolismo , Transglutaminases/metabolismo , Iogurte/microbiologia , Ácidos e Sais Biliares , Cápsulas , Liofilização/métodos , Suco Gástrico , Humanos , Concentração de Íons de Hidrogênio , Secreções Intestinais , Soja/química
8.
Int J Clin Exp Pathol ; 8(4): 4204-12, 2015.
Artigo em Inglês | MEDLINE | ID: mdl-26097613

RESUMO

Increasing evidences suggest that p120 catenin (p120ctn) exerts important functions in the regulation of pro-inflammatory molecules. However, the relationship among p120ctn, inflammatory responses and blood-brain barrier (BBB) dysfunction as they are the initiator of sepsis is not unknown. In this study, we found that p120ctn expression was correlated with an increase in the permeability of BBB and a decrease in the expression of tight-junction proteins in human brain microvascular endothelial cells (HBMECs) after LPS challenge. Transfection with p120ctn small interfering RNA (siRNA) induced disruption of BBB integrity, monocyte migration across BBB and inflammatory responses at basal level and after LPS treatment. Conversely, over-expression of p120ctn with adenovirus significantly ameliorated BBB disruption and inflammatory responses in LPS-treated cells. Mechanistically, up-regulation of p120ctn inhibited LPS-induced NF-κB activation by suppressing IKKß and IκBα phosphorylation, IκBα degradation. Therefore, we conclude that p120ctn improves the BBB dysfunction and inflammatory responses through the inhibition of NF-κB activation, suggesting that forced p120ctn expression may provide a novel therapeutic strategy to attenuate LPS-induced BBB compromise and sepsis.


Assuntos
Barreira Hematoencefálica/efeitos dos fármacos , Permeabilidade Capilar/efeitos dos fármacos , Cateninas/metabolismo , Inflamação/prevenção & controle , Lipopolissacarídeos/toxicidade , Microvasos/efeitos dos fármacos , Barreira Hematoencefálica/metabolismo , Cateninas/genética , Linhagem Celular , Humanos , Quinase I-kappa B/metabolismo , Proteínas I-kappa B/metabolismo , Inflamação/induzido quimicamente , Inflamação/genética , Inflamação/metabolismo , Microvasos/metabolismo , Inibidor de NF-kappaB alfa , NF-kappa B/metabolismo , Fosforilação , Proteólise , Interferência de RNA , Transdução de Sinais/efeitos dos fármacos , Proteínas de Junções Íntimas/metabolismo , Fatores de Tempo , Transfecção
9.
J Food Sci ; 80(6): M1259-65, 2015 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-25962443

RESUMO

A novel multi-cereal-based fermented beverage with suitable aroma, flavor, and pH fermented by lactic acid bacteria and Saccharomyces cerevisiae was developed. Twenty-seven lactobacilli strains were screened for acid production (pH and titratable acidity) in a mixture of malt, rice, and maize substrates. It was found that Lactobacillus helveticus KLDS1.9204 had the greatest acid production among 27 lactobacilli tested. The fermentation performance of L. helveticus KLDS1.9204 was also assayed and the fermentation parameters were optimized using Plackett-Burman design and steepest ascent method. L. helveticus KLDS1.9204 showed good proteolytic capability, however, the strain could not utilize starch. The optimum substrate consisted of 50% malt (25 g/100 mL), 25% rice (20 g/100 mL), and 25% maize (30 g/100 mL). The inoculum was 5% with a ratio of S. cerevisiae to L. helveticus KLDS1.9204 of 2.5:1. The optimum temperature was 37 °C and the time was 22 h. Lastly, the quality of the multi-cereal-based fermented beverage was evaluated. This beverage was light yellow, transparent, and it tasted well with a pleasant acid and a unique flavor of cereals. The beverage was rich in free amino acids and organic acids. The pH and titratable acidity of the beverage were 3.5 and 29.86 °T, respectively. The soluble solids content of the beverage was 6.5 °Brix, and the alcohol content was 0.67%.


Assuntos
Bebidas/análise , Grão Comestível , Fermentação , Microbiologia de Alimentos , Lactobacillus helveticus/metabolismo , Saccharomyces cerevisiae/metabolismo , Paladar , Ácidos/metabolismo , Aminoácidos/análise , Bebidas/microbiologia , Reatores Biológicos , Grão Comestível/química , Grão Comestível/microbiologia , Etanol/análise , Humanos , Concentração de Íons de Hidrogênio , Odorantes , Oryza , Temperatura Ambiente , Zea mays
10.
Mol Plant ; 8(6): 847-59, 2015 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-25747845

RESUMO

Bread wheat (or common wheat, Triticum aestivum) is an allohexaploid (AABBDD, 2n = 6x = 42) that arose by hybridization between a cultivated tetraploid wheat T. turgidum (AABB, 2n = 4x = 28) and the wild goatgrass Aegilops tauschii (DD, 2n = 2x = 14). Polyploidization provided niches for rigorous genome modification at cytogenetic, genetic, and epigenetic levels, rendering a broader spread than its progenitors. This review summarizes the latest advances in understanding gene regulation mechanisms in newly synthesized allohexaploid wheat and possible correlation with polyploid growth vigor and adaptation. Cytogenetic studies reveal persistent association of whole-chromosome aneuploidy with nascent allopolyploids, in contrast to the genetic stability in common wheat. Transcriptome analysis of the euploid wheat shows that small RNAs are driving forces for homoeo-allele expression regulation via genetic and epigenetic mechanisms. The ensuing non-additively expressed genes and those with expression level dominance to the respective progenitor may play distinct functions in growth vigor and adaptation in nascent allohexaploid wheat. Further genetic diploidization of allohexaploid wheat is not random. Regional asymmetrical gene distribution, rather than subgenome dominance, is observed in both synthetic and natural allohexaploid wheats. The combinatorial effects of diverged genomes, subsequent selection of specific gene categories, and subgenome-specific traits are essential for the successful establishment of common wheat.


Assuntos
Pão/análise , Poliploidia , Triticum/genética , Genes Sintéticos , Genoma de Planta , Hibridização Genética
11.
J Food Sci ; 80(1): M129-36, 2015 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-25533352

RESUMO

Traditional sufu is fermented by back-slopping and back-slopping has many defects. The objective of this study was to apply autochthonous mixed starter to control Kedong sufu fermentation. Sufu was manufactured using back-slopping (batch A) and autochthonous mixed starter (batch B) with Kocuria kristinae F7, Micrococcus luteus KDF1, and Staphylococcus carnosus KDFR1676. Considering physicochemical properties of sufu, 150-day sufu samples from batch A and 90-day sufu samples from batch B met the standard requirements, respectively. Considering sensory characteristics of sufu, 150-day sufu samples from batch A and 90-day sufu samples from batch B showed no significant differences (P > 0.05). The maturation period of sufu was shortened by 60 d. Profiles of free amino acids and peptides partly revealed the mechanism of typical sensory quality and shorter ripening time of sufu manufactured by autochthonous mixed starter. In final products, content of total biogenic amines was reduced by 48%. Autochthonous mixed starter performed better than back-slopping. Fermentation had a positive influence on the quality, safety, and sensory properties of sufu. The application of autochthonous mixed starter does not change the sensory characteristics of traditional fermented sufu. In addition, it reduces maturation period and improves their homogeneity and safety. It is possible to substitute autochthonous mixed starter for back-slopping in the manufacture of sufu.


Assuntos
Fermentação , Manipulação de Alimentos/métodos , Alimentos de Soja/análise , Alimentos de Soja/microbiologia , Aminoácidos/análise , China , Microbiologia de Alimentos , Micrococcaceae/metabolismo , Micrococcus luteus/metabolismo , Peptídeos/análise , Sensação , Staphylococcus/metabolismo
12.
J Zhejiang Univ Sci B ; 15(4): 333-42, 2014 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-24711354

RESUMO

This study proposed a quenching protocol for metabolite analysis of Lactobacillus delbrueckii subsp. bulgaricus. Microbial cells were quenched with 60% methanol/water, 80% methanol/glycerol, or 80% methanol/water. The effect of the quenching process was assessed by the optical density (OD)-based method, flow cytometry, and gas chromatography-mass spectrometry (GC-MS). The principal component analysis (PCA) and orthogonal partial least squares-discriminant analysis (OPLS-DA) were employed for metabolite identification. The results indicated that quenching with 80% methanol/water solution led to less damage to the L. bulgaricus cells, characterized by the lower relative fraction of prodium iodide (PI)-labeled cells and the higher OD recovery ratio. Through GC-MS analysis, higher levels of intracellular metabolites (including focal glutamic acid, aspartic acid, alanine, and AMP) and a lower leakage rate were detected in the sample quenched with 80% methanol/water compared with the others. In conclusion, we suggested a higher concentration of cold methanol quenching for L. bulgaricus metabolomics due to its decreasing metabolite leakage.


Assuntos
Lactobacillus delbrueckii/metabolismo , Metabolômica/métodos , Fermentação , Citometria de Fluxo , Microbiologia de Alimentos , Cromatografia Gasosa-Espectrometria de Massas , Análise dos Mínimos Quadrados , Metaboloma , Metabolômica/estatística & dados numéricos , Metanol , Análise de Componente Principal
13.
PLoS One ; 9(1): e84781, 2014.
Artigo em Inglês | MEDLINE | ID: mdl-24454749

RESUMO

MADS-box genes are important transcription factors for plant development, especially floral organogenesis. Brachypodium distachyon is a model for biofuel plants and temperate grasses such as wheat and barley, but a comprehensive analysis of MADS-box family proteins in Brachypodium is still missing. We report here a genome-wide analysis of the MADS-box gene family in Brachypodium distachyon. We identified 57 MADS-box genes and classified them into 32 MIKC(c)-type, 7 MIKC*-type, 9 Mα, 7 Mß and 2 Mγ MADS-box genes according to their phylogenetic relationships to the Arabidopsis and rice MADS-box genes. Detailed gene structure and motif distribution were then studied. Investigation of their chromosomal localizations revealed that Brachypodium MADS-box genes distributed evenly across five chromosomes. In addition, five pairs of type II MADS-box genes were found on synteny blocks derived from whole genome duplication blocks. We then performed a systematic expression analysis of Brachypodium MADS-box genes in various tissues, particular floral organs. Further detection under salt, drought, and low-temperature conditions showed that some MADS-box genes may also be involved in abiotic stress responses, including type I genes. Comparative studies of MADS-box genes among Brachypodium, rice and Arabidopsis showed that Brachypodium had fewer gene duplication events. Taken together, this work provides useful data for further functional studies of MADS-box genes in Brachypodium distachyon.


Assuntos
Brachypodium/genética , Genes de Plantas/genética , Proteínas de Domínio MADS/genética , Família Multigênica , Arabidopsis/efeitos dos fármacos , Arabidopsis/genética , Brachypodium/efeitos dos fármacos , Temperatura Baixa , Sequência Conservada/genética , Secas , Duplicação Gênica/genética , Regulação da Expressão Gênica de Plantas/efeitos dos fármacos , Genes Duplicados , Variação Genética , Motivos de Nucleotídeos/genética , Oryza/efeitos dos fármacos , Oryza/genética , Filogenia , Cloreto de Sódio/farmacologia , Estresse Fisiológico/efeitos dos fármacos , Estresse Fisiológico/genética
14.
Chin J Integr Med ; 20(6): 425-9, 2014 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-22539198

RESUMO

OBJECTIVE: To evaluate whether garlicin can prevent reperfusion no-reflow in a catheter-based porcine model of acute myocardial infarction (AMI). METHODS: Twenty-two male Chinese mini swines were randomized into 3 groups: sham-operation group (n=6), control group (n=8), and garlicin group (n=8). The distal part of left anterior descending coronary artery (LAD) in swines of the latter two groups was completely occluded by dilated balloon for 2 h and a successful AMI model was confirmed by coronary angiography (CAG) and electrocardiograph (ECG), which was then reperfused for 3 h. In the sham-operation group, balloon was placed in LAD without dilatation. Garlicin at a dosage of 1.88 mg/kg was injected 10 min before LAD occlusion until reperfusion for 1 h in the garlicin group. To assess serial cardiac function, hemodynamic data were examined by catheter method before AMI, 2 h after occlusion and 1, 2, and 3 h after reperfusion. Myocardial contrast echocardiography (MCE) and double staining with Evans blue and thioflavin-S were performed to evaluate myocardial no-reflow area (NRA) and risk area (RA). RESULTS: Left ventricular systolic pressure and left ventricular end-diastolic pressure significantly improved in the garlicin group after reperfusion compared with the control group P<0.05) and 2 h after AMI (P<0.05). MCE showed garlicin decreased reperfusion NRA after AMI compared with the control group (P <0.05). In double staining, NRA/RA in the garlicin group was 18.78%, significantly lower than that of the control group (49.84%, P<0.01). CONCLUSIONS: Garlicin has a preventive effect on the porcine model of myocardial infarction reperfusion no-reflow by improving hemodynamics and decreasing NRA.


Assuntos
Compostos Alílicos/uso terapêutico , Cardiotônicos/uso terapêutico , Dissulfetos/uso terapêutico , Infarto do Miocárdio/tratamento farmacológico , Reperfusão Miocárdica , Fenômeno de não Refluxo/tratamento farmacológico , Compostos Alílicos/farmacologia , Animais , Cardiotônicos/farmacologia , Meios de Contraste , Modelos Animais de Doenças , Dissulfetos/farmacologia , Hemodinâmica/efeitos dos fármacos , Masculino , Infarto do Miocárdio/complicações , Infarto do Miocárdio/diagnóstico por imagem , Infarto do Miocárdio/patologia , Fenômeno de não Refluxo/complicações , Fenômeno de não Refluxo/diagnóstico por imagem , Fenômeno de não Refluxo/patologia , Suínos , Porco Miniatura , Tiazóis/metabolismo , Ultrassonografia
15.
Iran J Allergy Asthma Immunol ; 13(1): 26-32, 2014 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-24338225

RESUMO

Cow's milk allergy is one of the most common food allergies in childhood. Beta-lactoglobulin (ß- lg) is a dominant allergen in cow's milk. Hydrolysis is known as an effective method to reduce the allergenicity of proteins. Thus, the objective of this study was to compare the allergenicity of ß-lg and its hydrolysates using an animal model. Twenty four BALB/c mice were divided into three groups and subcutaneously injected with native bovine ß-lg and its hydrolysates on days 0, 7 and 14. During the sensitization period, a number of systemic anaphylactic indicators were observed in mice sensitized by ß-lg compared to those sensitized by hydrolysates of ß-lg. Mice sensitized by hydrolysates of ß-lg showed a significantly lower spleen lymphocyte proliferation level than that sensitized by intact ß-lg. Antibody levels of ß-lg-specific IgE in serum induced by native ß-lg were significantly high. Plasma histamine levels were also evaluated and showed the same trend as IgE. Moreover, the hydrolysates of ß-lg significantly down-regulated IL-4 and IL-5 secretions in serum. These results suggested that enzymatic hydrolysis could reduce the allergenicity of ß-lg.


Assuntos
Fatores Imunológicos/farmacologia , Lactoglobulinas/farmacologia , Linfócitos/imunologia , Hipersensibilidade a Leite/imunologia , Hidrolisados de Proteína/farmacologia , Baço/imunologia , Animais , Bovinos , Proliferação de Células/efeitos dos fármacos , Regulação para Baixo/efeitos dos fármacos , Regulação para Baixo/imunologia , Feminino , Humanos , Imunoglobulina E/imunologia , Fatores Imunológicos/imunologia , Interleucina-4/imunologia , Interleucina-5/imunologia , Lactoglobulinas/imunologia , Linfócitos/patologia , Camundongos , Camundongos Endogâmicos BALB C , Hipersensibilidade a Leite/patologia , Hidrolisados de Proteína/imunologia , Baço/patologia
16.
Biol Pharm Bull ; 36(2): 202-7, 2013.
Artigo em Inglês | MEDLINE | ID: mdl-23207873

RESUMO

The present study was planned to explore the effect of Lactobacillus (L.) acidophilus on the T helper-17 (Th17) immune response in a mouse model of ß-lactoglobulin (ß-lg) allergy. Bovine ß-lg sensitised BALB/c mice were orally administered with different doses of heat-killed L. acidophilus (low, 5×10(7) colony forming unit (CFU); medium, 5×10(8) CFU; high, 5×10(9) CFU) in 200 µL of phosphate buffered saline (PBS) three times a week, starting from 1 week before ß-lg sensitisation for 4 weeks. After the allergen challenge, the numbers of blood eosinophils and neutrophils were examined by light microscope; the levels of cytokine (interleukin (IL)-12, IL-4, tumor growth factor (TGF)-ß, IL-10, IL-6 and IL-17A), total immunoglobulin E (IgE) and ß-lg-specific IgE contents in the serum were measured with enzyme-linked immunosorbent assay (ELISA); The mRNA expression levels of TGF-ß, IL-17A,CD25, Foxp3, retinoic acid-related orphan receptor γt (RORγt) and IL-10 were analyzed using real-time polymerase chain reaction (PCR). The results showed that oral administration of L. acidophilus suppressed hypersensitivity responses, attenuated the numbers of inflammatory cells and inhibited IgE production. We found up-regulation of TGF-ß and down-regulation of IL-17A in the serum of L. acidophilus-treated group, along with IL-6 levels was significantly decreased than that of the allergy group (p<0.05). Moreover, the mRNA expression levels of CD25, forkhead box P3 and TGF-ß were significantly higher in the spleen of L. acidophilus-treated group, while the mRNA expression levels of IL-17A, RORγt and IL-10 were significantly lower than that in the allergy group (p<0.05). In conclusion, the suppression of major allergic symptoms by oral administration of L.acidophilus was probably due to improve the regulatory T (Treg)/Th17 balance and inhibit the IL-6 production.


Assuntos
Alérgenos/efeitos adversos , Antialérgicos/administração & dosagem , Lactobacillus acidophilus , Lactoglobulinas/efeitos adversos , Hipersensibilidade a Leite/terapia , Administração Oral , Animais , Bovinos , Citocinas/sangue , Citocinas/genética , Feminino , Expressão Gênica , Temperatura Alta , Imunoglobulina E/sangue , Subunidade alfa de Receptor de Interleucina-2/genética , Contagem de Leucócitos , Camundongos , Camundongos Endogâmicos BALB C , Hipersensibilidade a Leite/etiologia , Hipersensibilidade a Leite/imunologia , Membro 3 do Grupo F da Subfamília 1 de Receptores Nucleares/genética , Células Th17/imunologia , Fator de Crescimento Transformador beta/genética
17.
Hum Mol Genet ; 21(9): 2132-41, 2012 May 01.
Artigo em Inglês | MEDLINE | ID: mdl-22323360

RESUMO

Genome-wide association studies have identified susceptibility loci for esophageal squamous cell carcinoma (ESCC). We conducted a meta-analysis of all single-nucleotide polymorphisms (SNPs) that showed nominally significant P-values in two previously published genome-wide scans that included a total of 2961 ESCC cases and 3400 controls. The meta-analysis revealed five SNPs at 2q33 with P< 5 × 10(-8), and the strongest signal was rs13016963, with a combined odds ratio (95% confidence interval) of 1.29 (1.19-1.40) and P= 7.63 × 10(-10). An imputation analysis of 4304 SNPs at 2q33 suggested a single association signal, and the strongest imputed SNP associations were similar to those from the genotyped SNPs. We conducted an ancestral recombination graph analysis with 53 SNPs to identify one or more haplotypes that harbor the variants directly responsible for the detected association signal. This showed that the five SNPs exist in a single haplotype along with 45 imputed SNPs in strong linkage disequilibrium, and the strongest candidate was rs10201587, one of the genotyped SNPs. Our meta-analysis found genome-wide significant SNPs at 2q33 that map to the CASP8/ALS2CR12/TRAK2 gene region. Variants in CASP8 have been extensively studied across a spectrum of cancers with mixed results. The locus we identified appears to be distinct from the widely studied rs3834129 and rs1045485 SNPs in CASP8. Future studies of esophageal and other cancers should focus on comprehensive sequencing of this 2q33 locus and functional analysis of rs13016963 and rs10201587 and other strongly correlated variants.


Assuntos
Carcinoma de Células Escamosas/genética , Cromossomos Humanos Par 2/genética , Neoplasias Esofágicas/genética , Grupo com Ancestrais do Continente Asiático/genética , China , Cromossomos Humanos Par 10/genética , Predisposição Genética para Doença , Variação Genética , Estudo de Associação Genômica Ampla , Haplótipos , Humanos , Polimorfismo de Nucleotídeo Único , Recombinação Genética
18.
J Zhejiang Univ Sci B ; 12(8): 638-43, 2011 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-21796804

RESUMO

OBJECTIVE: To evaluate whether liposomal prostaglandin E1 (lipo-PGE1) can decrease reperfusion no-reflow in a catheter-based porcine model of acute myocardial infarction (AMI). METHODS: Twenty-two male Chinese mini-swines were randomized into three groups: six in a sham-operation group, and eight each in the control and lipo-PGE1 groups. The distal part of the left anterior descending coronary artery (LAD) in the latter two groups was completely occluded for 2 h, and then reperfused for 3 h. Lipo-PGE1 (1 µg/kg) was injected 10 min before LAD occlusion until reperfusion for 1 h in the lipo-PGE1 group. Hemodynamic data and proinflammatory cytokines were examined before AMI, 2 h after occlusion, and 1, 2, and 3 h after reperfusion. Myocardial contrast echocardiography (MCE) and double staining were performed to evaluate the myocardial no-reflow area (NRA). RESULTS: Left ventricular systolic pressure and end-diastolic pressure significantly improved in the lipo-PGE1 group after reperfusion compared with the control group and also 2 h after AMI (P<0.05 for both). MCE and double staining both showed that lipo-PGE1 decreased reperfusion NRA after AMI (P<0.05, P<0.01). Lipo-PGE1 decreased serum interleukin-6 (IL-6) and tumor necrosis factor-α (TNF-α) after myocardial infarction reperfusion (P<0.05 for both). CONCLUSIONS: Lipo-PGE1 is cardioprotective in our porcine model of myocardial infarction reperfusion no-reflow, decreasing NRA and attenuating the inflammatory response.


Assuntos
Alprostadil/metabolismo , Lipossomos/metabolismo , Infarto do Miocárdio/metabolismo , Algoritmos , Animais , Vasos Coronários/patologia , Modelos Animais de Doenças , Ecocardiografia/métodos , Interleucina-6/metabolismo , Masculino , Reperfusão Miocárdica , Miocárdio/metabolismo , Radioimunoensaio/métodos , Distribuição Aleatória , Suínos , Porco Miniatura , Fatores de Tempo , Fator de Necrose Tumoral alfa/metabolismo
19.
Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi ; 27(4): 389-91, 394, 2011 Apr.
Artigo em Chinês | MEDLINE | ID: mdl-21481313

RESUMO

AIM: To analyse the effect of 7 Lactobacillus species on proliferation and cytokine production of primary lymphocytes, and explored the effect on the Th1/Th2 cells balance. METHODS: The mouse spleen cells were stimulated with active/heat-killed Lactobacillus strains at different species and concentrations. After be cultured with bacteria 60 hours, the proliferation of splenocytes were tested by MTT colorimetry, and IL-12, IFN-γ, IL-4, IL-10 and TGF-ß were detected by ELISA. RESULTS: (1) Both alive and heat-killed Lactobacillus species could accelerate the proliferation of spleen cells in a dose-dependent manner (P<0.05), the immuneological activity of heat-killed L.fermentum and L.acidophilu were not inferior to the live strains, especially when treated at higher dose(10(7);CFU/mL, bacteria: cell ratio of 10:1). Furthermore, these two heat-killed bacterial strains suppressed IL-4, IL-10 and TGF-ß production by stimulating IL-12 and IFN-γ production, and their IFN-γ/IL-4 value which represent the Th1/Th2 balance were higher than ConA control group significantly(P<0.05). CONCLUSION: The results indicate that Lactobacillus strains may differ in their respective ability to improve Th1/Th2 cells balance toward Th1 dominance via increasing the secretion ratio of IFN-γ/IL-4 (Th1/Th2) by splenocytes.


Assuntos
Lactobacillus/imunologia , Células Th1/imunologia , Células Th2/imunologia , Animais , Feminino , Interferon gama/biossíntese , Interleucina-4/biossíntese , Ativação Linfocitária , Camundongos , Camundongos Endogâmicos BALB C
20.
Clin Exp Pharmacol Physiol ; 36(9): 933-9, 2009 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-19473338

RESUMO

1. Hypertrophic cardiomyopathy (HCM) is a genetic disorder that has a complex set of symptoms and potentially devastating consequences. Increasing evidence indicates that mitochondrial DNA (mtDNA) mutations are responsible for the development of HCM, but the mtDNA mutations appear to differ considerably among different populations and regions. 2. In the present study, three families with HCM were found and investigated: one in Shandong province and two in the Chongqing region of China. The entire mtDNA genome from the 18 affected and 66 unaffected family members was sequenced directly and the mtDNA mutations were determined. 3. The frequency of haplogroup M10 was significantly higher in family members with HCM (HCM group) than in unaffected family members (normal group). Three mtDNA mutations were found with a significantly higher frequency in affected individuals than in unaffected family individuals, namely G7697A in the cytochrome c oxidase subunit II gene (P < 0.0001; odds ratio (OR) 227.5; 95% confidence interval (CI) 23.6­2194.8) and T12477C (P = 0.0037; OR 5.6; 95% CI 1.8­17.6) and G13135A in the NADH dehydrogenase 5 gene (P < 0.0001; OR 26.0; 95% CI 6.9­98.3), suggesting that these mutations are probably associated with susceptibility to HCM. In addition, mitochondrial Complex I activity was markedly decreased in the HCM group, suggesting that these mutations most likely affect mitochondrial respiratory function. 4. In conclusion, the results of the present study imply that mtDNA mutations G7697A, T12477C and G13135A are genetic factors that indicate a susceptibility to HCM and that could be used for the large-scale screening of genetic markers as well as the early diagnosis of HCM.


Assuntos
Grupo com Ancestrais do Continente Asiático/genética , Cardiomiopatia Hipertrófica Familiar/genética , DNA Mitocondrial , Complexo IV da Cadeia de Transporte de Elétrons/genética , Complexo I de Transporte de Elétrons/genética , Proteínas Mitocondriais/genética , Mutação , Adolescente , Adulto , Idoso , Cardiomiopatia Hipertrófica Familiar/diagnóstico por imagem , Cardiomiopatia Hipertrófica Familiar/etnologia , Cardiomiopatia Hipertrófica Familiar/metabolismo , Cardiomiopatia Hipertrófica Familiar/fisiopatologia , Estudos de Casos e Controles , Distribuição de Qui-Quadrado , China/epidemiologia , Análise Mutacional de DNA , Complexo I de Transporte de Elétrons/metabolismo , Feminino , Frequência do Gene , Predisposição Genética para Doença , Haplótipos , Hereditariedade , Humanos , Masculino , Pessoa de Meia-Idade , Razão de Chances , Linhagem , Fenótipo , Medição de Risco , Fatores de Risco , Ultrassonografia , Adulto Jovem
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