Your browser doesn't support javascript.
loading
Mostrar: 20 | 50 | 100
Resultados 1 - 20 de 616
Filtrar
1.
Nat Commun ; 12(1): 5908, 2021 Oct 08.
Artigo em Inglês | MEDLINE | ID: mdl-34625564

RESUMO

Oncolytic herpes simplex virus-1 is capable of lysing tumor cells while alerting the immune system. CD47, in collaboration with SIRPα, represents an important immune checkpoint to inhibit phagocytosis by innate immune cells. Here we show locoregional control of glioblastoma by an oncolytic herpes virus expressing a full-length anti(α)-human CD47 IgG1 or IgG4 antibody. The antibodies secreted by the virus-infected glioblastoma cells block the CD47 'don't eat me' signal irrespective of the subclass; however, αCD47-IgG1 has a stronger tumor killing effect than αCD47-IgG4 due to additional antibody-dependent cellular phagocytosis by macrophages and antibody-dependent cellular cytotoxicity by NK cells. Intracranially injected αCD47-IgG1-producing virus continuously releases the respective antibody in the tumor microenvironment but not into systemic circulation; additionally, αCD47-IgG1-producing virus also improves the survival of tumor-bearing mice better than control oncolytic herpes virus combined with topical αCD47-IgG1. Results from immunocompetent mouse tumor models further confirm that macrophages, and to a lesser extent NK cells, mediate the anti-tumor cytotoxicity of antibody-producing oncolytic herpesviruses. Collectively, oncolytic herpes simplex virus-1 encoding full-length antibodies could improve immune-virotherapy for glioblastoma.

2.
J Environ Manage ; 301: 113856, 2021 Oct 06.
Artigo em Inglês | MEDLINE | ID: mdl-34626948

RESUMO

The conventional sintering process of municipal solid waste incineration (MSWI) fly ash is always energy intensive. The process forms a cracked structure because of the difficulty in forming the liquid phase to enhance the mass transfer process. Therefore, exploring a new disposal method to simultaneously decrease the sintering temperature and improve the mechanical and heavy metal leaching properties of sintered samples is necessary. In this study, a pressure-assisted sintering treatment was introduced to dispose fly ash by varying the chemical composition and mechanical pressure at relatively low temperatures (300-500 °C). The results revealed that the compressive strength of treated samples increased with the CaO/SiO2 molar ratio increasing from 0.5 to 1.0, and a maximum value of 238.28 ± 8.50 MPa was obtained. The heavy metal leaching concentration results demonstrated a low risk of contamination in the treated samples. Microstructure analyses suggested that the densification process was enhanced with increased mechanical pressure, and the formed calcium silicates and aluminosilicates positively affected the compressive strength. Moreover, smaller crystal lattices were observed during aggregation formation, suggesting the restraint of anomalous crystal growth, which accelerated the densification process and increased the compressive strength. Moreover, the mass transfer process during the pressure-assisted sintering process was enhanced compared with the conventional thermal process, which was reflected by the transformation of elements from homogeneous to heterogeneous distribution. Therefore, the improved mechanical properties and leaching behavior of heavy metals were attributed to the densified microstructure, formation of new minerals, and enhanced driving force during the pressure-assisted sintering process. These findings suggest that pressure-assisted sintering is a promising method for maximizing the reutilization and minimizing the energy consumption simultaneously to dispose fly ash.

3.
J Virol ; : JVI0096421, 2021 Oct 20.
Artigo em Inglês | MEDLINE | ID: mdl-34668775

RESUMO

A comprehensive analysis and characterization of a severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection model that mimics non-severe and severe coronavirus disease 2019 (COVID-19) in humans is warranted for understating the virus and developing preventive and therapeutic agents. Here, we characterized the K18-hACE2 mouse model expressing human (h)ACE2 in mice, controlled by the human keratin 18 (K18) promoter, in the epithelia, including airway epithelial cells where SARS-CoV-2 infections typically start. We found that intranasal inoculation with higher viral doses (2×103 and 2×104 PFU) of SARS-CoV-2 caused lethality of all mice and severe damage of various organs, including lung, liver, and kidney, while lower doses (2×101 and 2×102 PFU) led to less severe tissue damage and some mice recovered from the infection. In this hACE2 mouse model, SARS-CoV-2 infection damaged multiple tissues, with a dose-dependent effect in most tissues. Similar damage was observed in post-mortem samples from COVID-19 patients. Finally, the mice that recovered from infection with a low dose of virus survived rechallenge with a high dose of virus. Compared to other existing models, the K18-hACE2 model seems to be the most sensitive COVID-19 model reported to date. Our work expands the information available about this model to include analysis of multiple infectious doses and various tissues with comparison to human post-mortem samples from COVID-19 patients. In conclusion, the K18-hACE2 mouse model recapitulates both severe and non-severe COVID-19 in humans being dose-dependent and can provide insight into disease progression and the efficacy of therapeutics for preventing or treating COVID-19. Importance The pandemic of coronavirus disease 2019 (COVID-19) has reached nearly 240 million cases and caused nearly 5 million deaths worldwide as of October 2021, has raised an urgent need for the development of novel drugs and therapeutics to prevent the spread and pathogenesis of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). To achieve this goal, an animal model that recapitulates the features of human COVID-19 disease progress and pathogenesis is greatly needed. In this study, we have comprehensively characterized a mouse model of SARS-CoV-2 infection using K18-hACE2 transgenic mice. We infected the mice with low and high doses of SARS-CoV-2 to study the pathogenesis and survival in response to different infection patterns. Moreover, we compared the pathogenesis of the K18-hACE2 transgenic mice with that of the COVID-19 patients to show that this model could be a useful tool for the development of anti-viral drugs and therapeutics.

4.
Inorg Chem ; 2021 Sep 23.
Artigo em Inglês | MEDLINE | ID: mdl-34555280

RESUMO

[Nb(η6-C6H3Me3)2] reacts with ethylenediamine (en) solutions of K4Ge9 in the presence of 18-crown-6 to give [(η6-C6H3Me3)NbHGe6]2- (1) and [(η6-C6H3Me3)NbGe6Nb(η6-C6H3Me3)]2- (2) as their corresponding [K(18-crown-6)]+ salts. The crystalline solids are dark brown, air-sensitive, and sparingly soluble or insoluble in most solvents. The [K(18-crown-6)]+ salts of cluster ions 1 and 2 have been characterized by energy-dispersive X-ray (EDX) analysis, NMR studies, single-crystal X-ray diffraction, and electrospray ionization time-of-flight (ESI-TOF) mass spectrometry studies. Cluster ions 1 and 2 have markedly different [Ge6] moieties: an electron-deficient carborane-like subunit in 1 and a two-center, two-electron cyclohexane-like subunit in 2.

5.
Cancer Lett ; 521: 196-209, 2021 Sep 03.
Artigo em Inglês | MEDLINE | ID: mdl-34481934

RESUMO

Previous reports have shown that histone deacetylase inhibitors (HDACi) can alter miRNA expression in a range of cancers. Both the 5p-arm and 3p-arm of mature miRNAs can be expressed from the same precursor and involved in cancer progress. Nevertheless, the detailed mechanism by which vorinostat (SAHA), a HDACi, triggers miR-769-5p/miR-769-3p-mediated suppression of proliferation and induces apoptosis in gastric cancer (GC) cells remains elusive. Here, we showed that the miRNA-seq analysis of GC cells treated with SAHA identified seven differentially expressed miRNAs with both strands of the miRNA duplex. miR-769-5p/miR-769-3p expression was downregulated in GC tissues compared with normal tissues. Functionally, high expression of miR-769-5p/miR-769-3p blocked the malignant abilities of GC cells. Mechanistically, miR-769-5p/miR-769-3p targeted IGF1R and IGF1R overexpression rescued the effects of miR-769-5p/miR-769-3p on GC cells growth and metastasis. Moreover, STAT3 bound to the promoter of miR-769. Furthermore, miR-769-5p/miR-769-3p expression was negatively regulated by the STAT3-IGF1R-HDAC3 complex. Besides, miR-769-5p/miR-769-3p synergized with SAHA to promote GC cells apoptosis. Our studies suggest that miR-769-5p/miR-769-3p acts as a tumor suppressor by the STAT3-IGF1R-HDAC3 complex. Moreover, SAHA triggers miR-769-5p/miR-769-3p-mediated inhibition of proliferation and induces apoptosis in GC cells.

6.
Aging (Albany NY) ; 13(18): 22459-22473, 2021 Sep 23.
Artigo em Inglês | MEDLINE | ID: mdl-34554926

RESUMO

Emerging studies have revealed that non-coding RNAs contribute to regulating intervertebral disc degeneration (IVDD). Here, we intended to probe into the function of miR-19b-3p in IVDD evolvement. The miR-19b-3p level in the intervertebral disc (IVD) tissues of IVDD patients and IL-1ß/TNF-α/hydrogen peroxide-treated human nucleus pulposus cells (HNPCs) was determined by quantitative real-time polymerase chain reaction (qRT-PCR). Also, qRT-PCR was conducted to examine the profiles of MMP-3, MMP-9, MMP-13, ADAMTS-4 and ADAMTS-5. The PTEN/PI3K/Akt/mTOR pathway was examined by Western blot (WB). The miR-19b-3p overexpression assay was carried out, and HNPC proliferation and apoptosis were compared by the cell counting kit-8 (CCK-8) assay and flow cytometry (FCM). In addition, the mechanism of action of miR-19b-3p was clarified using the PTEN inhibitor (VO-Ohpic triphosphate) or the mTOR inhibitor (Rapamycin) on the basis of IL-1ß intervention and miR-19b-3p mimics transfection. Our results testified that miR-19b-3p expression was curbed in IVD tissues of the IVDD patients (vs. normal IVD tissues) and IL-1ß-, TNF-α, or hydrogen peroxide-treated HNPCs. Up-regulating miR-19b-3p enhanced HNPC proliferation and hampered its apoptosis. Moreover, miR-19b-3p dampened the PTEN profile and activated the PI3K/Akt/mTOR pathway. Interestingly, attenuating PTEN reduced IL-1ß-, TNF-α-, or hydrogen peroxide-mediated HNPC apoptosis and up-regulated PI3K/Akt/mTOR, while inhibiting the mTOR pathway offset the protective function of miR-19b-3p. Further mechanism studies illustrated that miR-19b-3p targeted the 3'untranslated region (UTR) of PTEN and abated the PTEN level. This research confirmed that miR-19b-3p suppressed HNPC apoptosis in the in-vitro model of IVDD by regulating PTEN/PI3K/Akt/mTOR pathway.

7.
Can J Gastroenterol Hepatol ; 2021: 9990338, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34557456

RESUMO

Long noncoding RNAs (lncRNAs) have been substantially reported to have critical roles in regulating tumorigenesis in recent years. However, the expression pattern and biological function of SNHG17 in hepatocellular carcinoma (HCC) remain unclear. Bioinformatics analysis and qRT-PCR were performed to detect the expression pattern of SNHG17 in HCC tissues, adjacent nontumorous tissues, and cell lines. The effect of SNHG17 on proliferation, migration, and apoptosis of HCC was investigated by knockdown and overexpressing SNHG17 in HCC cell lines. RNA sequencing was utilized to explore the underlying mechanism. Utilizing publicly available TCGA-LIHC, GSE102079 HCC datasets, and qRT-PCR, we found SNHG17 was significantly upregulated in HCC tissues and cell lines and was notably associated with larger tumor size, poorly differentiation, presence of vascular invasion, and advanced TNM stage. Furthermore, gain- and loss-of-function studies demonstrated that SNHG17 promoted cell proliferation and migration and inhibited apoptosis of HCC. By employing RNA sequencing, we found knockdown of SNHG17 caused 1037 differentially expressed genes, highly enriched in several pathways, including metabolic, PI3K-Akt, cell adhesion, regulation of cell proliferation, and apoptotic pathway; among them, 92 were overlapped with SNHG17-related genes in the TCGA-LIHC dataset. Furthermore, ERH, TBCA, TDO2, and PDK4 were successfully validated and found significantly dysregulated in HCC tissues. Moreover, HCC patients with higher SNHG17 expression had a relatively poor overall survival and disease-free survival, and ERH and PDK4 also played a marked role in the prognosis of HCC. Broadly, our findings illustrate that SNHG17 acts as a noncoding oncogene in HCC progression, suggesting its potential value as a novel target for HCC therapy.


Assuntos
Carcinoma Hepatocelular , Neoplasias Hepáticas , RNA Longo não Codificante , Carcinoma Hepatocelular/genética , Proliferação de Células/genética , Regulação Neoplásica da Expressão Gênica , Humanos , Neoplasias Hepáticas/genética , Fosfatidilinositol 3-Quinases/metabolismo , Prognóstico , RNA Longo não Codificante/genética
8.
Waste Manag ; 134: 21-31, 2021 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-34403993

RESUMO

Municipal solid waste incineration (MSWI) fly ash disposal is an urgent task with some technical bottlenecks. In this study, a novel pressure-assisted sintering method was employed to treat the MSWI fly ash. A series of pressure-assisted sintering experiments were carried out by varying mechanical pressures and sintering temperatures, and their properties of compressive strength, density and heavy metals leaching behavior were determined to screen out the optimal conditions. Instrumental analysis of XRF, SEM, XRD and TEM-EDX and calculation kinetics were conducted to explore the enhancement mechanism of pressure-assisted sintering. With the help of mechanical pressure, a high-strength ceramic product was produced from MSWI fly ash sintered at a low temperature (400 °C), which never occurred in the conventional low-temperature sintering process. Maximum compressive strength of 218.30 ± 4.08 MPa was obtained at 400 °C and 100 MPa, which was much higher than conventional construction materials of brick and cement. In addition, the leaching concentrations of heavy metals obtained from pressure-assisted sintering process were lower than the standard limitation. The SEM and XRD results revealed that the increased mechanical properties and the decreased heavy metals leaching concentration were mainly attributed to the increased density and crystalline degree. The kinetics calculation results indicated that the sintering activation energy was much lower than the sintering process without pressure, suggesting surface diffusion and grain boundary diffusion were main sintering mechanisms in the pressure-assisted sintering process. These findings proved that pressure-assisted sintering could be a promising method to treat fly ash together with producing high-value building materials.


Assuntos
Metais Pesados , Eliminação de Resíduos , Carbono , Cinza de Carvão , Incineração , Metais Pesados/análise , Material Particulado , Resíduos Sólidos/análise , Temperatura
9.
Theranostics ; 11(16): 8112-8128, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34335983

RESUMO

The coiled-coil domain containing protein members have been well documented for their roles in many diseases including cancers. However, the function of the coiled-coil domain containing 65 (CCDC65) remains unknown in tumorigenesis including gastric cancer. Methods: CCDC65 expression and its correlation with clinical features and prognosis of gastric cancer were analyzed in tissue. The biological role and molecular basis of CCDC65 were performed via in vitro and in vivo assays and a various of experimental methods including co-immunoprecipitation (Co-IP), GST-pull down and ubiquitination analysis et al. Finally, whether metformin affects the pathogenesis of gastric cancer by regulating CCDC65 and its-mediated signaling was investigated. Results: Here, we found that downregulated CCDC65 level was showed as an unfavourable factor in gastric cancer patients. Subsequently, CCDC65 or its domain (a.a. 130-484) was identified as a significant suppressor in GC growth and metastasis in vitro and in vivo. Molecular basis showed that CCDC65 bound to ENO1, an oncogenic factor has been widely reported to promote the tumor pathogenesis, by its domain (a.a. 130-484) and further promoted ubiquitylation and degradation of ENO1 by recruiting E3 ubiquitin ligase FBXW7. The downregulated ENO1 decreased the binding with AKT1 and further inactivated AKT1, which led to the loss of cell proliferation and EMT signal. Finally, we observed that metformin, a new anti-cancer drug, can significantly induce CCDC65 to suppress ENO1-AKT1 complex-mediated cell proliferation and EMT signals and finally suppresses the malignant phenotypes of gastric cancer cells. Conclusion: These results firstly highlight a critical role of CCDC65 in suppressing ENO1-AKT1 pathway to reduce the progression of gastric cancer and reveals a new molecular mechanism for metformin in suppressing gastric cancer. Our present study provides a new insight into the mechanism and therapy for gastric cancer.


Assuntos
Biomarcadores Tumorais/metabolismo , Proteínas de Ligação a DNA/metabolismo , Glicoproteínas/metabolismo , Fosfopiruvato Hidratase/metabolismo , Proteínas Proto-Oncogênicas c-akt/metabolismo , Neoplasias Gástricas/metabolismo , Proteínas Supressoras de Tumor/metabolismo , Animais , Linhagem Celular Tumoral , Movimento Celular/genética , Proliferação de Células/genética , China , Feminino , Expressão Gênica/genética , Regulação Neoplásica da Expressão Gênica/genética , Genes Supressores de Tumor/fisiologia , Glicoproteínas/genética , Humanos , Masculino , Metformina/metabolismo , Metformina/farmacologia , Camundongos , Camundongos Endogâmicos BALB C , Camundongos Nus , Oncogenes , Prognóstico , Proteínas Proto-Oncogênicas c-akt/fisiologia , Transdução de Sinais/genética , Neoplasias Gástricas/genética , Neoplasias Gástricas/patologia , Ubiquitina-Proteína Ligases/metabolismo , Ubiquitinação
10.
Bioresour Technol ; 341: 125752, 2021 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-34419878

RESUMO

Integrating hydrothermal carbonization (HTC) and anaerobic digestion for biorefinery-oriented full utilization of wet organic wastes is a promising emerging technology. The objectives of this study were to identify the potential inhibitory substances, evaluate the biomethane potential of mixed and aqueous products and explore process intensifying strategies. The results indicated that the high HTC temperature of 240 °C resulted in a significantly low methane yield of 60 ± 5 mL/g COD and a high Short chain fatty acid (SCFAs) accumulation of 4174 ± 76 mg/L. GC-MS analysis showed that the contents of inhibitory pyrazines, pyridines and ketones in aqueous fraction at 240 °C substantially increased from 13.14%, 0.4%, 0.55% at 180 °C to 23.34%, 2.89%, 5.13%, respectively. When the aqueous products obtained from 240 °C-HTC was supplemented or pretreated by carbonaceous material, the methane yields were greatly improved and increased to 1.3-fold and 1.8-fold, respectively. These finding could provide some valuable technical information for HTC based biorefinery of organic waste.


Assuntos
Carbono , Metano , Anaerobiose , Temperatura , Água
11.
Sci Total Environ ; 796: 148716, 2021 Nov 20.
Artigo em Inglês | MEDLINE | ID: mdl-34274676

RESUMO

Improving sludge drying efficiency is of tremendous importance for public health, subsequent treatment, and comprehensive utilization. The interfacial thermal resistance between sludge and hot wall greatly limits the conductive drying performance. This study employed mechanical compression to decrease the interfacial thermal resistance. The drying kinetics and interfacial heat transfer behavior were investigated at mechanical loads of 25 to 200 kPa, temperatures of 120 to 210 °C, and sludge thicknesses of 1.0 to 3.0 mm, and were compared to those in the conventional drying process without mechanical load. The increase of temperature and mechanical load and the decrease of thickness improved drying rates. The drying experienced one warm-up period and two falling rate periods. The breakthrough of interfacial vapor film was responsible for the rapid rise in drying rates initially. At the thickness of 3.0 mm, 210 °C, and 100 kPa, the effective moisture diffusivity was increased by 2.5 times, and the apparent activation energy was reduced by 34% compared to the traditional process in the first falling rate period, implying that mechanical compression facilitated moisture migration and bound water desorption. The effective moisture diffusivity in the first falling rate period was increased by 35% compared to the diffusivity in the second falling rate period because of the pressure-driven flow. The decrease in drying rates was due to the transformation from the pressure-driven flow to vapor diffusion-limited flow in the first falling rate period. Additionally, this study provided essential information on developing a new sludge treatment method and establishing the drying model.


Assuntos
Temperatura Alta , Esgotos , Dessecação , Gases , Cinética
12.
Waste Manag ; 132: 96-104, 2021 Aug 01.
Artigo em Inglês | MEDLINE | ID: mdl-34325332

RESUMO

Gasification of wet municipal solid waste (MSW) coupled with in-situ CO2 capture is an attractive option for MSW disposal, allowing chemical and energy recovery. In this study, the Ni-CaO based catalysts were prepared with waste marble powder (WMP) as an alternative to CaO and promoted by different transition metals (i.e., Fe, Cu, Co and Zn). The bimetallic catalysts were prepared by the impregnation method and characterized by different analytical techniques. The catalyst performance for wet MSW gasification was evaluated in a fixed-bed reactor at optimized conditions (850 °C and 50% moisture content of MSW). The results revealed that the addition of Ni-WMP catalyst greatly enhanced the dry gas yield (DGY), H2 yield, carbon conversion efficiency (CCE) and reduced the tar content from 0.73 to 1.16 N.m3/kg, 212 to 509 mL/g, 61.70% to 76.40% and 9.11 to 3.9 wt%, respectively, compared to without catalyst. In contrast to the Ni-WMP catalyst, the transition metal promoted catalysts showed higher catalytic activity towards H2 yield (549-629 mL/g), DGY (1.19-1.30 N.m3/kg), and lower tar content (3.45-2.93 wt%). The results revealed that Co promoted bimetallic catalyst performed better than Fe, Cu and Zn promoted catalysts. The tar content produced was also analyzed via GC-MS (gas chromatography-mass spectrometry) to understand the effect of different catalysts on tar composition. According to experimental results, the bimetallic promoted catalysts can be ranked as Ni-Co-WMP > Ni-Cu-WMP > Ni-Fe-WMP > Ni-Zn-WMP based on H2 yield and tar removal.


Assuntos
Hidrogênio , Resíduos Sólidos , Carbonato de Cálcio , Catálise , Pós
13.
Aging (Albany NY) ; 13(12): 16043-16061, 2021 06 24.
Artigo em Inglês | MEDLINE | ID: mdl-34167089

RESUMO

HMGA1 protein is an architectural transcription factor that has been implicated in the progression of multiple malignant tumors. However, the role of HMGA1 in the growth and metastasis of gastric cancer (GC) has not yet been elucidated. Here, we show that HMGA1 is overexpressed in GC cells and the high expression of HMGA1 was correlated with worse survival in GC patients using a bioinformatics assay. Functionally, HMGA1 affected the EdU incorporation, colony formation, migration and invasion of GC cells by exogenously increasing or decreasing the expression of HMGA1. Mechanistically, HMGA1 directly bound to the SUZ12 and CCDC43 promoter and transactivated its expression in GC cells. Inhibition of SUZ12 and CCDC43 attenuated the proliferation, migration and invasiveness of HMGA1-overexpressing GC cells in vitro. Moreover, both HMGA1 and SUZ12/CCDC43 were highly expressed in cancer cells but not in normal gastric tissues, and their expressions were positively correlated. Finally, a tail vein metastatic assay showed that HMGA1 promoted SUZ12/CCDC43-mediated GC cell metastasis in vivo. Our findings suggest that HMGA1 promotes GC growth and metastasis by transactivating SUZ12 and CCDC43 expression, highlighting HMGA1 as a potential prognostic biomarker in the treatment of GC.


Assuntos
Proteína HMGA1a/metabolismo , Proteínas de Neoplasias/genética , Neoplasias Gástricas/genética , Neoplasias Gástricas/patologia , Fatores de Transcrição/genética , Ativação Transcricional/genética , Animais , Carcinogênese/genética , Carcinogênese/patologia , Linhagem Celular Tumoral , Proliferação de Células/genética , Progressão da Doença , Regulação Neoplásica da Expressão Gênica , Humanos , Camundongos , Metástase Neoplásica , Proteínas de Neoplasias/metabolismo , Regiões Promotoras Genéticas/genética , Ligação Proteica , Fatores de Transcrição/metabolismo , Regulação para Cima/genética
14.
Hum Mol Genet ; 30(19): 1881-1893, 2021 Sep 15.
Artigo em Inglês | MEDLINE | ID: mdl-34104955

RESUMO

The etiology of cleft lip with/without cleft palate (CL/P), one of the most frequent craniofacial birth defects worldwide, is complicated by contributions of both genetic and environmental factors. Understanding the etiology of these conditions is essential for developing preventive strategies. This study thus aims to identify regulatory networks of microRNAs (miRNAs), transcriptional factors (TFs) and non-TF genes associated with cleft lip (CL) that are conserved in humans and mice. Notably, we found that miR-27b, miR-133b, miR-205, miR-376b and miR-376c were involved in the regulation of CL-associated gene expression in both humans and mice. Among the candidate miRNAs, the overexpression of miR-27b, miR-133b and miR-205, but not miR-376b and miR-376c, significantly inhibited cell proliferation through suppression of CL-associated genes (miR-27b suppressed PAX9 and RARA; miR-133b suppressed FGFR1, PAX7, and SUMO1; and miR-205 suppressed PAX9 and RARA) in cultured human and mouse lip mesenchymal cells. Taken together, our results suggest that elevated expression of miR-27b, miR-133b and miR-205 may play a crucial role in CL through the suppression of genes associated with CL.

15.
Chemosphere ; 280: 130937, 2021 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-34162109

RESUMO

Nitrate and sulfate often coexist in organic wastewater. In this study, an internal circulation anaerobic reactor was conducted to investigate the impact of nitrate on sulfate reduction. The results showed that sulfate reduction rate dropped from 78.4% to 41.4% at NO3- /SO42- ratios ranging from 0 to 1.03, largely attributed to the inactivity of acetate-utilizing sulfate-reducing bacteria (SRB) and preferential usage of nitrate of propionate-utilizing SRB. Meanwhile, high nitrate removal efficiency was maintained and COD removal efficiency increased with nitrate addition. Enhancement of propionate and butyrate degradation based on Modified Gompertz model and Phylogenetic Investigation of Communities by Reconstruction of Unobserved States (PICRUSt2) analysis. Moreover, nitrate triggered the shift of microbial community and function. Twelve genera affiliated to Firmicutes, Bacteroidetes and Proteobacteria were identified as keystone genera via network analysis, which kept functional stability of the bacterial community responding to nitrate stress. Increased nitrate inhibited Desulfovibrio, but promoted the growth of Desulforhabdus. Both the predicted functional genes associated with assimilatory sulfate reduction pathway (cysC and cysNC) and dissimilatory sulfate reduction pathway (aprA, aprB, dsrA and dsrB) exhibited negative relationship with nitrate addition.


Assuntos
Desulfovibrio , Nitratos , Anaerobiose , Reatores Biológicos , Oxirredução , Filogenia , Sulfatos
16.
Chem Commun (Camb) ; 57(37): 4496-4499, 2021 May 06.
Artigo em Inglês | MEDLINE | ID: mdl-33956016

RESUMO

A new superphane, featuring an aesthetically pleasing structure, was successfully obtained via one-pot synthesis of a hexakis-amine and m-phthalaldehyde in a [2+6] manner. It proved capable of entrapping a water dimer within its cavity as inferred from the mass spectroscopy, crystallographical analysis, NMR spectroscopy, and theoretical calculations.


Assuntos
Aldeídos/síntese química , Aminas/síntese química , Teoria da Densidade Funcional , Água/química , Aldeídos/química , Aminas/química , Cápsulas , Dimerização , Espectroscopia de Ressonância Magnética , Estrutura Molecular
17.
Clin Interv Aging ; 16: 801-810, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34017170

RESUMO

Background and Purpose: Elevated high-density lipoprotein cholesterol (HDL-C) levels have displayed protection against cardiovascular disease. However, the association between specific lipoprotein classes and first ischemic stroke (IS) has not been well defined, particularly in higher-risk hypertensive populations. Our study evaluated the associations of HDL-C with first IS in a Chinese hypertensive population. Methods: The study population was obtained from a community-based cohort study of hypertension in Lianyungang and Rongcheng, China. A nested case-control design was used that included 2463 identified first IS cases and 2463 controls matched by age ± 1 year, sex, and region. Results: After adjusting for potential confounders, HDL-C was inversely associated with first IS (adjusted odds ratio [aOR]: 0.91; 95% confidence interval [CI]: 0.85-0.98). HDL-C levels of at least 65.4 mg/dL displayed a significant protective effect for first IS (aOR: 0.82; 95% CI: 0.69-0.98). Conversely, adverse effects of first IS were observed for low-density lipoprotein cholesterol (LDL-C) levels ≥138.1 mg/dL (aOR: 1.20; 95% CI: 1.02-1.42) and triglyceride (TG) levels ≥140.8 mg/dL (aOR: 1.27; 95% CI: 1.09-1.49). The risk associations of LDL-C and TG with first IS were attenuated in the presence of high HDL-C (≥53.0 mg/dL); an increased risk of first IS was only found in the presence of low HDL-C (<53.0 mg/dL) when LDL-C (aOR: 1.66; 95% CI: 1.19-2.31) and TG (aOR: 1.47; 95% CI: 1.17-1.84) were combined with HDL-C for analysis. Conclusion: In this community-based Chinese hypertensive population, higher HDL-C was a significant protective factor of first IS. These data add to the evidence describing the relationship between lipids and IS and suggest that HDL-C maybe is a marker of IS risk in Chinses hypertensive population.


Assuntos
HDL-Colesterol/sangue , Hipertensão/epidemiologia , AVC Isquêmico/epidemiologia , Idoso , Biomarcadores , Estudos de Casos e Controles , China/epidemiologia , Feminino , Humanos , Hipertensão/fisiopatologia , AVC Isquêmico/fisiopatologia , Lipídeos/sangue , Masculino , Pessoa de Meia-Idade , Razão de Chances , Fatores de Risco
18.
J Coll Physicians Surg Pak ; 30(5): 605-607, 2021 May.
Artigo em Inglês | MEDLINE | ID: mdl-34027881

RESUMO

The aim of this study was to investigate the role of TFH cells and interleukin (IL)-21 in the pathogenesis of childhood Mycoplasma pneumoniae pneumonia (MPP). It was a retrospective study carried out at Yantai Yuhuangding Hospital, China from July 2016 to June 2019. It comprised 46 healthy control (HC) children and 106 pediatric patients, including 60 children with general MPP (GMPP) and 46 children with refractory MPP (RMPP). Flow cytometry was used to detect the frequencies of peripheral TFH cells. The levels of serum IL-21 were tested by ELISA. The transcriptional factors Bcl-6 and Blimp-1 mRNA levels were examined using RT-qPCR. The percentage of TFH cells and expression levels of IL-21 and Bcl-6 in peripheral blood were all significantly elevated in MPP groups compared with HC group (all p <0.001). Furthermore, it was found that a significantly higher percentage of TFH cells and levels of IL-21 and Bcl-6 in RMPP children, compared with GMPP children (all p <0.05). In contrast, lower Blimp-1 levels were observed in MPP groups (p <0.001), especially in RMPP group (p = 0.021). Therefore, the elevated expansion of TFH cells and the increased secretion of IL-21 cytokine may contribute to the pathogenesis of childhood MPP, while its excessive response may be associated with the development of childhood RMPP. Key Words: TFH cells, IL-21, Children, Mycoplasma pneumoniae pneumonia.


Assuntos
Mycoplasma pneumoniae , Pneumonia por Mycoplasma , Criança , China , Humanos , Interleucinas/genética , Estudos Retrospectivos , Células T Auxiliares Foliculares
19.
Chemosphere ; 280: 130721, 2021 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-33962293

RESUMO

Poor effect of contaminants removal efficiency and low organic matter content of activated sludge are common in wastewater treatment plants (WWTPs) in China due to the low-strength wastewater. An anaerobic-anoxic-oxic (AAO) and an adsorption/AAO (A/AAO) combined system were established simultaneously to conduct a comparative study for realizing the conversion of carbon source in influent and the enrichment and recovery of proteins and amino acids through the assimilation of ammonia nitrogen. The experimental results showed that 63.5% of the organic matter in influent was adsorbed and flocculated in adsorption process, and the removal rates of chemical oxygen demand, total nitrogen and total phosphorus in A/AAO process were 88.7%, 77.1%, and 93.0% respectively, which were remarkably better than those in AAO process owing to the addition of improved carbon source. Ammonia assimilation rate of A/AAO process was 26.7% higher than that of AAO process, which implied that the ammonia used to synthesize sludge protein was prominently increased. Furthermore, intracellular proteins and amino acids in A/AAO process were 20% higher than those of AAO process, and the quality was equivalent with fish meal or soybean meal as feed. In addition, the microbial community analysis based on 16S rDNA was conducted. Dechloromonas, Zoogloea, Nitrospira, and Flavobacterium were the main genera, and played important roles in nutrient removal and ammonia nitrogen assimilation. The integration of adsorption process was significant to low-strength wastewater treatment and the improvement of excess sludge quality, which is a prospective inspiration for the resource recovery-based wastewater treatment process.


Assuntos
Amônia , Esgotos , Aminoácidos , Anaerobiose , Reatores Biológicos , China , Nitrogênio , Fósforo , Estudos Prospectivos , Eliminação de Resíduos Líquidos , Águas Residuárias
20.
J Hazard Mater ; 414: 125600, 2021 07 15.
Artigo em Inglês | MEDLINE | ID: mdl-34030425

RESUMO

Element-doped TiO2 nanotube arrays (TNAs) with optimized active sites provide an effective approach for significantly improving electrocatalytic performance. The challenges in such construction mainly include selection of green dopant and control of active sites. Herein, we present phytic acid as a phosphorus source for P-doped TNAs. An oxygen vacancy (Ov) and P co-doped TNAs (P-TiO2-y) was prepared as an electrochemical oxidation anode. P-TiO2-y exhibits excellent degradation activity due to the formation of Ti-O-P bonds and generation of Ov. P-doping was beneficial in improving the oxygen evolution potential of the electrode, which would be benefit for electrocatalytic degradation of pollutants. Using the P-TiO2-y anode with a current density of 10 mA/cm2 for tetracycline degradation, after a 3 h treatment, the removal rate, chemical oxygen demand and total organic carbon removal rates were 100%, 90.32% and 76.60%, respectively. The P-TiO2-y also has excellent degradation performance for phenol, hydroquinone, p-nitrophenol and metronidazole.

SELEÇÃO DE REFERÊNCIAS
DETALHE DA PESQUISA
...