Your browser doesn't support javascript.
Mostrar: 20 | 50 | 100
Resultados 1 - 20 de 247
Filtrar
Mais filtros










Base de dados
Intervalo de ano de publicação
1.
Ecotoxicol Environ Saf ; 188: 109906, 2020 Jan 30.
Artigo em Inglês | MEDLINE | ID: mdl-31708226

RESUMO

Despite the toxicity of mercury for mammal has been widely studied in recent years, little is known on its impact on the soil microbiome. In this paper, the effects of mercury in soils microbial communities along a gradient of contamination from no to high concentration was assessed by the richness and diversity of microbial community using high throughput sequencing method. The richness of microbial community decreased gradually with the increase of culture time, while the low and medium concentration of mercury had little effect on the evenness of soil microbial community. Proteobacteria tolerated the mercury contamination, while Acidobacteria, Planctomycetes and Chloroflexi were sensitive to mercury pollution in phylum level. Omnitrophica and Ignavibacteriae microorganisms were very sensitive to mercury contamination and dead quickly when contaminated with mercury. Mercury contamination selected two mercury tolerance genuses which were Massilia and Burkholderia in genus level and at least 22 microorganisms such as Alkanindiges, Geothrix, Polycyclovorans and Sporichthya in genus which mainly from the Acidobacteria, Proteobacteria, Bacteroides, Chloroflexi and Omnitrophica phylum were sensitive to mercury. The bacteria tolerant to mercury in soil were Massilia and Burkholderia from Betaproteobacteria and Lysobacter, Luteimonas from Gammaproteobacteria, separately, they were Gram-negative bacteria with thin cell walls and complex ingredients that responded quickly to pollution stress.

2.
Food Chem ; 308: 125650, 2020 Mar 05.
Artigo em Inglês | MEDLINE | ID: mdl-31655477

RESUMO

This study aimed at investigating the formation and accumulation of 16 reactive aldehydes in clam (Ruditapes philippinarum) during oil frying in both the tissue and the oil using an HPLC-ESI-MS/MS methodology. After processing, the accumulation of acrolein, crotonaldehyde, pentanal, trans-2-hexenal, hexanal, trans, trans-2,4-heptadienal, heptanal, nonanal, trans, trans-2,4-decadienal and 4-hydroxy-2-nonenal was most noticeable in both fried clam and frying oil. Most of the aldehyde species showed a time- and temperature-dependent manner of formation and accumulation during frying due to continuous oxidative degradation under conditions employed. However, several species of aldehyde such as acrolein and trans-2-pentenal slightly decreased at higher temperatures and/or longer frying times, which may be due to the imbalance toward disappearance of aldehydes resulting from their evaporation under the extreme conditions. Presence of natural polyphenols in bamboo leaves significantly prevented the formation of aldehydes in both fried clam and frying oil due to their antioxidant activity (P < 0.05).

3.
J Bone Joint Surg Am ; 101(22): 2015-2025, 2019 Nov 20.
Artigo em Inglês | MEDLINE | ID: mdl-31764364

RESUMO

BACKGROUND: The Judet-Letournel classification has been widely used to diagnose acetabular fractures since it was proposed. However, there has been growing evidence of incompleteness and comprehension difficulty in this classification, which may adversely affect its clinical use. The purposes of this study were to introduce a novel 3-column classification system for acetabular fractures and to evaluate its reliability and reproducibility. METHODS: A total of 1,028 patients with acetabular fractures, who had undergone surgical treatment from June 2011 to January 2017 in 7 level-I trauma centers, were recruited into this study. Preoperative radiographs and computed tomographic (CT) scans were conducted for each patient. To evaluate the reproducibility of the 3-column classification system for acetabular fractures, the interobserver and intraobserver reliability (kappa coefficient, κ) of this classification system compared with those of the Judet-Letournel classification system was investigated by 4 observers. RESULTS: A total of 209 patients (20.33%) could not be classified by the Judet-Letournel classification, and 3 cases (0.29%) could not be classified by the novel 3-column classification. The mean κ value of the interobserver reliability for the Judet-Letournel classification was 0.591 (range, 0.508 to 0.681), indicating moderate agreement, whereas the mean κ value was 0.735 (range, 0.594 to 0.930), indicating substantial agreement, when using the 3-column classification. The mean κ value for the intraobserver reliability was 0.751 (range, 0.708 to 0.793) for the Judet-Letournel classification and 0.909 (range, 0.792 to 0.957) for the 3-column classification. CONCLUSIONS: The 3-column classification, based on the anatomic character of the acetabulum, showed higher interobserver and intraobserver reliability than the Judet-Letournel classification. Additionally, certain fracture patterns in the 3-column classification scheme generally correlated with surgical approaches. This novel classification system may be used as a supplement to the traditional Judet-Letournel classification system. CLINICAL RELEVANCE: The 3-column concept of the acetabulum proposed in this study is helpful to master acetabular fractures for less experienced surgeons. The novel classification system could assist with acetabular fracture diagnosis and the choice of surgical approaches.

4.
Artigo em Inglês | MEDLINE | ID: mdl-31667573

RESUMO

PURPOSE: This study aimed to evaluate the clinical outcomes, toxicity, and prognostic factors of SBRT combined with gemcitabine plus capecitabine (GEM-CAP) in treating locally advanced pancreatic cancer (LAPC). METHODS: A total of 56 patients with LAPC treated with SBRT combined with GEM-CAP were reviewed from October 2010 to October 2016. The median total prescription dose at five fractions was 40 Gy (30-50 Gy). The patients were subjected to two cycles of GEM-CAP before SBRT. GEM-CAP chemotherapy was then offered for four cycles or until disease tolerance or progression. The primary endpoints included overall survival (OS) and progression-free survival (PFS). RESULTS: The median OS and PFS from the date of diagnosis was 19 (95% CI 14.6-23.4) and 12 months (95% CI 8.34-15.66), respectively. The 1-year and 2-year survival rates were 82.1% and 35.7%, whereas the 1-year and 2-year PFS rates were 48.2% and 14.3%, respectively. The median carbohydrate antigen 19-9-determined PFS time was 11 months (95% CI 5.77-16.24). Multivariate analysis demonstrated that tumor diameter, lymph node metastasis, pre-treatment CA19-9 level, and post-treatment CA19-9 decline were independent prognostic factors (p < 0.05). Acute toxicity was minimal, with two cases (3.6%) experiencing grade 3 duodenal obstruction. No adverse events greater than grade 3 occurred. In late toxicity, three patients (5.4%) developed grade 3 gastrointestinal toxicity and two (3.6%) suffered from perforation caused by grade 4 radiation enteritis and intestinal fistula. CONCLUSIONS: The combination of Cyberknife SBRT and GEM-CAP achieved excellent efficacy with acceptable toxicity for LAPC.

5.
Fish Physiol Biochem ; 2019 Nov 28.
Artigo em Inglês | MEDLINE | ID: mdl-31782040

RESUMO

In this study, the mechanism that VC inhibits lipid deposition through GSK-3ß/mTOR signaling was investigated in the liver of Danio rerio. The results indicated that 0.5- and 1.0-g/kg VC treatments activated mTOR signaling by inhibiting GSK-3ß expression. The mRNA expression of FAS, ACC, and ACL, as well as the content of TG, TC, and NEFA, was decreased by 0.5- and 1.0-g/kg VC treatments. Moreover, to confirm GSK-3ß playing a key role in regulating TSC2 and mTOR, GSK-3ß RNA was interfered and the activity of GSK-3ß was inhibited by 25- and 50-mg/L LiCl treatments, respectively. The results indicated that GSK-3ß inactivation played a significant role in inducing mTOR signaling and inhibiting lipid deposition. VC treatments could induce mTOR signaling by inhibiting GSK-3ß, and mTOR further participated in regulating lipid deposition by controlling lipid profile in the liver of zebrafish.

6.
J Bone Joint Surg Am ; 2019 Sep 20.
Artigo em Inglês | MEDLINE | ID: mdl-31567681

RESUMO

BACKGROUND: The Judet-Letournel classification has been widely used to diagnose acetabular fractures since it was proposed. However, there has been growing evidence of incompleteness and comprehension difficulty in this classification, which may adversely affect its clinical use. The purposes of this study were to introduce a novel 3-column classification system for acetabular fractures and to evaluate its reliability and reproducibility. METHODS: A total of 1,028 patients with acetabular fractures, who had undergone surgical treatment from June 2011 to January 2017 in 7 level-I trauma centers, were recruited into this study. Preoperative radiographs and computed tomographic (CT) scans were conducted for each patient. To evaluate the reproducibility of the 3-column classification system for acetabular fractures, the interobserver and intraobserver reliability (kappa coefficient, κ) of this classification system compared with those of the Judet-Letournel classification system was investigated by 4 observers. RESULTS: A total of 209 patients (20.33%) could not be classified by the Judet-Letournel classification, and 3 cases (0.29%) could not be classified by the novel 3-column classification. The mean κ value of the interobserver reliability for the Judet-Letournel classification was 0.591 (range, 0.508 to 0.681), indicating moderate agreement, whereas the mean κ value was 0.735 (range, 0.594 to 0.930), indicating substantial agreement, when using the 3-column classification. The mean κ value for the intraobserver reliability was 0.751 (range, 0.708 to 0.793) for the Judet-Letournel classification and 0.909 (range, 0.792 to 0.957) for the 3-column classification. CONCLUSIONS: The 3-column classification, based on the anatomic character of the acetabulum, showed higher interobserver and intraobserver reliability than the Judet-Letournel classification. Additionally, certain fracture patterns in the 3-column classification scheme generally correlated with surgical approaches. This novel classification system may be used as a supplement to the traditional Judet-Letournel classification system. CLINICAL RELEVANCE: The 3-column concept of the acetabulum proposed in this study is helpful to master acetabular fractures for less experienced surgeons. The novel classification system could assist with acetabular fracture diagnosis and the choice of surgical approaches.

7.
DNA Cell Biol ; 38(11): 1197-1206, 2019 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-31618067

RESUMO

Researches establish an indispensable role of mitochondrial dysfunction in septic cardiomyopathy. We aimed to investigate the effects of long noncoding RNA (LncRNA) SOX2 overlapping transcript (SOX2OT) on mitochondrial dysfunction in septic cardiomyopathy. We observed an obvious overexpression of SOX2OT in septic hearts and cardiomyocytes. Knockdown of SOX2OT in mice recovered the reduced cardiac function, and improved the mitochondrial membrane potential impaired by lipopolysaccharide (LPS). SOX2OT overexpressed mice showed the opposite situation. In parallel, knockdown of SOX2OT in cardiomyocytes restored the mitochondrial membrane potential, along with reduced mitochondrial reactive oxygen species production induced by LPS, while overexpression of SOX2OT reversed these effects. Mechanistically, SOX2OT could regulate mitochondrial dysfunction in septic cardiomyopathy via SOX2. In general, SOX2OT contributed to mitochondrial dysfunction progression via inhibiting SOX2 expression in septic cardiomyopathy, which may provide a new insight for treatment of septic cardiomyopathy.


Assuntos
Cardiomiopatias , Mitocôndrias Cardíacas/fisiologia , Doenças Mitocondriais/genética , RNA Longo não Codificante/fisiologia , Sepse , Animais , Cardiomiopatias/genética , Cardiomiopatias/patologia , Cardiomiopatias/fisiopatologia , Células Cultivadas , Masculino , Camundongos , Camundongos Endogâmicos C57BL , Mitocôndrias Cardíacas/patologia , Doenças Mitocondriais/patologia , Ratos , Sepse/genética , Sepse/patologia , Sepse/fisiopatologia
8.
Chem Commun (Camb) ; 55(83): 12455-12458, 2019 Oct 15.
Artigo em Inglês | MEDLINE | ID: mdl-31565704

RESUMO

Hypochlorous acid (HClO) is one of the most important reactive oxygen species (ROS) and plays a vital role in many physiological and pathological processes. The comprehensive exploration of mechanistic details and the potential clinical translation necessitate the development of reliable probes for prompt and accurate detection of HClO in complex biological environments. Herein we report a fluorinated bihydrazide conjugate as a 19F NMR/MRI probe with a "turn-on" character for the detection of HClO. This probe could selectively respond to HClO, leading to a significant recovery of 19F signals for 19F NMR/MRI. Activatable sensing and imaging of HClO were achieved with SMMC-7721 cells and nude mice, which demonstrates that this small molecular conjugate could serve as a selective probe for real-time sensing and imaging of HClO in biological systems.


Assuntos
Corantes Fluorescentes/química , Hidrazinas/química , Ácido Hipocloroso/análise , Imagem por Ressonância Magnética , Animais , Linhagem Celular Tumoral , Radioisótopos de Flúor , Halogenação , Humanos , Espectroscopia de Ressonância Magnética , Camundongos , Camundongos Nus , Espécies Reativas de Oxigênio
9.
Bioinformatics ; 2019 Sep 17.
Artigo em Inglês | MEDLINE | ID: mdl-31584615

RESUMO

MOTIVATION: Allele dropout (ADO) and unbalanced amplification of alleles are main technical issues of single-cell sequencing (SCS), and effectively emulating these issues is necessary for reliably benchmarking SCS-based bioinformatics tools. Unfortunately, currently available sequencing simulators are free of whole-genome amplification involved in SCS technique and therefore not suited for generating SCS datasets. We develop a new software package (SCSsim) that can efficiently simulate SCS datasets in a parallel fashion with minimal user intervention. SCSsim first constructs the genome sequence of single cell by mimicking a complement of genomic variations under user-controlled manner, and then amplifies the genome according to MALBAC technique and finally yields sequencing reads from the amplified products based on inferred sequencing profiles. Comprehensive evaluation in simulating different ADO rates, variation detection efficiency and genome coverage demonstrates that SCSsim is a very useful tool in mimicking single-cell sequencing data with high efficiency. AVAILABILITY AND IMPLEMENTATION: SCSsim is freely available at https://github.com/qasimyu/scssim. SUPPLEMENTARY INFORMATION: Supplementary data are available at Bioinformatics online.

10.
Bioinformatics ; 2019 Oct 18.
Artigo em Inglês | MEDLINE | ID: mdl-31626284

RESUMO

MOTIVATION: Detecting driver genes from gene mutation data is a fundamental task for tumorigenesis research. Due to the fact that cancer is a heterogeneous disease with various subgroups, subgroup specific driver genes are the key factors in the development of precision medicine for heterogeneous cancer. However, the existing driver gene detection methods are not designed to identify subgroup specificities of their detected driver genes, and therefore cannot indicate which group of patients are associated with the detected driver genes, which is difficult to provide specifically clinical guidance for individual patients. RESULTS: By incorporating the subspace learning framework, we propose a novel bioinformatics method called DriverSub, which can efficiently predict subgroup specific driver genes in the situation where the subgroup annotations are not available. When evaluated by simulation datasets with known ground truth and compared with existing methods, DriverSub yields the best prediction of driver genes and the inference of their related subgroups. When we apply DriverSub on the mutation data of real heterogeneous cancers, we can observe that the predicted results of DriverSub are highly enriched for experimentally validated known driver genes. Moreover, the subgroups inferred by DriverSub are significantly associated with the annotated molecular subgroups, indicating its capability of predicting subgroup specific driver genes. AVAILABILITY: The source code are publicly available at https://github.com/JianingXi/DriverSub. SUPPLEMENTARY INFORMATION: Supplementary data are available at Bioinformatics online.

11.
Math Biosci Eng ; 16(5): 5972-5990, 2019 Jun 28.
Artigo em Inglês | MEDLINE | ID: mdl-31499748

RESUMO

In this work, an SIRS model with age structure is proposed for recurrent infectious disease by incorporating temporary immunity and delay. We formulate the model as an abstract non-densely defined Cauchy problem and derive the conditions for the global stability of disease free equilibrium, the local stability of endemic equilibrium, and the existence of Hopf bifurcation. Both non-periodic and periodic behaviors are possible when the disease persists in population, where time delay plays an important role. Numerical examples are provided to illustrate our theoretical results.

12.
Environ Pollut ; 254(Pt A): 112968, 2019 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-31554144

RESUMO

This study analyzed the effect of heavy metal eluents (0.3 mol/L C6H8O7, 5 × 10-4 mol/L EDTA, and 0.01 mol/L Na2S2O3) on the content of organic matter, hydrolytic nitrogen, available phosphorus and potassium, and species composition of bacteria and fungi in vegetable soils. The obtained results documented that the treatment of the soil, consisting of shaking the sample with a mixture of eluents, significantly increased the content of organic matter, hydrolytic nitrogen, and available phosphorus and potassium. The mixed solutions of eluents increase the maximum available P in the soil by 279.3%, and hydrolytic N by 30.7%. The eluents affected, to a certain extent, the dominant species of microorganisms in the soil, but did not increase species richness and evenness in all soil samples.


Assuntos
Bactérias/crescimento & desenvolvimento , Fungos/crescimento & desenvolvimento , Metais Pesados/análise , Poluentes do Solo/análise , Solo/química , Bactérias/metabolismo , Fungos/metabolismo , Nitrogênio/análise , Fósforo/análise , Microbiologia do Solo
13.
Angew Chem Int Ed Engl ; 58(45): 16110-16114, 2019 Nov 04.
Artigo em Inglês | MEDLINE | ID: mdl-31518048

RESUMO

The design and synthesis of uranium sorbent materials with high uptake efficiency, capacity and selectivity, as well as excellent hydrolytic stability and radiation resistance remains a challenge. Herein, a polyoxometalate (POM)-organic framework material (SCU-19) with a rare inclined polycatenation structure was designed, synthesized through a solvothermal method, and tested for uranium separation. Under dark conditions, SCU-19 can efficiently capture uranium through ligand complexation using its exposed oxo atoms and partial chemical reduction from UVI to UIV by the low-valent Mo atoms in the POM. An additional UVI photocatalytic reduction mechanism can occur under visible light irradiation, leading to a higher uranium removal without saturation and faster sorption kinetics. SCU-19 is the only uranium sorbent material with three distinct sorption mechanisms, as further demonstrated by X-ray photoelectron spectroscopy (XPS) and X-ray absorption near edge structure (XANES) analysis.

14.
Brain Behav Immun ; 82: 354-371, 2019 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-31513876

RESUMO

Stroke is a leading cause of long-term disability worldwide; survivors often show sensorimotor and cognitive deficits. Therapeutic exercise is the most common treatment strategy for rehabilitating patients with stroke via augmentation of neurogenesis, angiogenesis, neurotrophic factors expression, and synaptogenesis. Neurogenesis plays important roles in sensorimotor and cognitive functional recovery, and can be promoted by exercise; however, the mechanism underlying this phenomenon remains unclear. In this study, we explored the effects of treadmill exercise on sensorimotor and cognitive functional recovery, as well as the potential molecular mechanisms underlying the promotion of neurogenesis in a rat model of transient middle cerebral artery occlusion (tMCAO). We found that treadmill exercise facilitated sensorimotor and cognitive functional recovery after tMCAO, and that neural stem/progenitor cell proliferation, differentiation, and migration were enhanced in the ipsilateral subventricular and subgranular zones after tMCAO. Meanwhile, the newborn neurons induced by treadmill exercise after tMCAO had the similar function with pre-existing neurons. Treadmill exercise significantly increased CD200 and CD200 receptor (CD200R) levels in the ipsilateral hippocampus and cortex. Further study revealed that treadmill exercise-induced neurogenesis and functional recovery were clearly inhibited, while Il-ß and Tnf-α expression were upregulated, following lentivirus (LV)-induced suppression of post-stroke CD200R expression. Consistent with the effect of treadmill exercise, CD200Fc (a CD200R agonist) markedly promoted neurogenesis and functional recovery after stroke. In addition, CD200Fc could further enhance the functional recovery induced by treadmill exercise after stroke. Our results demonstrate the beneficial role of treadmill exercise in promoting neurogenesis and functional recovery via activating the CD200/CD200R signaling pathway and improving the inflammatory environment after stroke. Thus, the CD200/CD200R signaling pathway is a potential therapeutic target for functional recovery after stroke.

15.
Mar Biotechnol (NY) ; 21(5): 613, 2019 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-31385167

RESUMO

The original version of this article unfortunately contained a mistake in the authorgroup section. Letters A, B, C, D, E and F were inadvertently added before each author's name. The original article has been corrected.

16.
J Hazard Mater ; 381: 120952, 2019 Jul 31.
Artigo em Inglês | MEDLINE | ID: mdl-31400715

RESUMO

MXene/chitosan nanocoating for flexible polyurethane foam (PUF) was prepared via layer-by-layer (LbL) approach. MXene (Ti3C2) ultra-thin nanosheets were obtained through etching process of Ti3AlC2 followed by exfoliation. The deposition of MXene/chitosan nanocoating was conducted by alternatingly immersing the PUF into a chitosan solution and a Ti3C2 aqueous dispersion, which resulted in different number of bilayers (BL) ranging from 2, 5 and 8. Owing to the utilization of ultra-thin Ti3C2 nanosheets, the weight gain was only 6.9% for 8 BL coating of PUF, which minimised the unfavourable impact on the intrinsic properties of PUF. The Ti3C2/chitosan coating significantly reduced the flammability and smoke releases of PUF. Compared with unmodified PUF, the 8 BL coating reduced the peak heat release rate by 57.2%, alongside with a 65.5% reduction in the total heat release. The 8 BL coating also showed outstanding smoke suppression ability with total smoke release decreased by 71.1% and peak smoke production rate reduced by 60.3%, respectively. The peak production of CO and CO2 gases also decreased by 70.8% and 68.6%, respectively. Furthermore, an outstanding char formation performance of 37.2 wt.% residue was obtained for 8 BL coated PUF, indicating the excellent barrier and carbonization property of the hybrid coating.

17.
Ecotoxicol Environ Saf ; 183: 109517, 2019 Nov 15.
Artigo em Inglês | MEDLINE | ID: mdl-31394377

RESUMO

The study aimed to determine the biochar yield of four aquatic plants, the leaching toxicity of copper (Cu) and cadmium (Cd) in the biochar, and the stabilization characteristics of the biochar produced under different pyrolysis conditions (at 350 °C for 1, 2, and 3 h and absence/presence of zeolite powder). The results showed that different plant species required a different pyrolysis duration and the presence or absence of zeolite powder. The stabilization of Cu and Cd was significantly affected by the pyrolysis duration and the external materials for different plant species and different types of admixtures. Pyrolysis temperatures over 350 °C for 1 h without zeolite powder generated stable Cu and Cd in goldfish algae (Ceratophyllum demersum L.), foxtail algae (Myriophyllum verticillatum L.), and penny grass (Hydrocotyle vulgaris). Pyrolysis temperatures over 350 °C for 1 h with zeolite powder made Cu and Cd stable in water celery (Oenanthe javanica (Bl.) DC). The addition of zeolite powder during pyrolysis was possible due to the weight reduction efficiency in plants with Cu and Cd. Furthermore, the surface of the biochar with the zeolite powder showed honeycombs and a spongy porous structure. The duration of the pyrolysis had little effect on the honeycomb pore structure.


Assuntos
Organismos Aquáticos/química , Cádmio/análise , Carvão Vegetal/química , Cobre/análise , Pirólise , Poluentes Químicos da Água/análise , Zeolitas/química , Biomassa , Cádmio/toxicidade , Cobre/toxicidade , Temperatura Alta , Oenanthe/química , Saxifragales/química , Fatores de Tempo , Poluentes Químicos da Água/toxicidade
18.
Waste Manag ; 98: 135-143, 2019 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-31446253

RESUMO

The predominant heavy metals in MSWI fly ash and electrolytic manganese residue (EMR) were determined to be Zn, Pb, Cd, and Mn, with lesser amounts of Cu and Cr. The curing efficiency of heavy metals in MSWI fly ash and EMR was improved using complex alkaline activators (NaOH and KOH), base addition (calcium hydroxide and complex Portland cement), and EMR calcining (at 800 °C for 3 h) based on a geopolymeric system. The best formulation of the geopolymeric system was composed of 75 wt% MSWI fly ash and 25 wt% EMR with a KOH/NaOH (1:1) complex solution (7.5 M OH-)/solid of 0.5. Calcium ions were dissolved aluminosilicate under the strongly basic conditions to form complex products (ternesite) which further improved the strength. The primary curing mechanism of heavy metals (Pb, Zn, Cd, Mn, Cr, and Cu) mainly was primarily influenced by the acid-base buffering capacity of geopolymers, followed by the physical encapsulation of geopolymeric gels.


Assuntos
Metais Pesados , Eliminação de Resíduos , Carbono , Cinza de Carvão , Incineração , Íons , Manganês , Material Particulado
19.
BMC Genomics ; 20(1): 625, 2019 Jul 31.
Artigo em Inglês | MEDLINE | ID: mdl-31366319

RESUMO

BACKGROUND: Oyster is rich in glycogen and free amino acids and is called "the milk of sea". To understand the main genetic effects of these traits and the genetic networks underlying their correlation, we have conducted the whole genome resequencing with 427 oysters collected from the world-wide scale. RESULTS: After association analysis, 168 clustered significant single nucleotide polymorphism (SNP) loci were identified for glycogen content and 17 SNPs were verified with 288 oyster individuals in another wide populations. These were the most important candidate loci for oyster breeding. Among 24 genes in the 100-kb regions of the leading SNP loci, cytochrome P450 17A1 (CYP17A1) contained a non-synonymous SNP and displayed higher expressions in high glycogen content individuals. This might enhance the gluconeogenesis process by the transcriptionally regulating the expression of phosphoenolpyruvate carboxykinase (PEPCK) and glucose 6-phosphatase (G6Pase). Also, for amino acids content, 417 clustered significant SNPs were identified. After genetic network analysis, three node SNP regions were identified to be associated with glycogen, protein, and Asp content, which might explain their significant correlation. CONCLUSION: Overall, this study provides insights into the genetic correlation among complex traits, which will facilitate future oyster functional studies and breeding through molecular design.

20.
Nano Lett ; 19(8): 5394-5402, 2019 08 14.
Artigo em Inglês | MEDLINE | ID: mdl-31286778

RESUMO

Multifunctional nanoplatforms featuring promising properties including excellent loading efficiency, real-time monitoring, and improved cargo bioavailability and bioselectivity are in great demand by the biomedical research community. During the development of such nanoplatforms, stimuli-responsive nanoparticles (NPs) as a smart nanoplatform have recently received extensive attention. Herein, we report small-sized octapod-shaped hollow porous manganese(II) oxide (HPMO) NPs as a stimuli-responsive T1-activatable nanoplatform for tumor-specific cargo delivery and real-time monitoring. The HPMO NPs functionalized by zwitterionic dopamine sulfonate (ZDS) can act as a versatile platform to load organic dyes or chemotherapeutic drugs with high loading efficiency. The obtained Cargo@HPMO would decompose into paramagnetic Mn2+ ions and subsequently release cargoes in mild acidic conditions, especially in tumor microenvironment and lysosome. The released Mn2+ can enhance T1 magnetic resonance signal for real-time monitoring of the cargo delivery in vivo. This octapod-shaped Cargo@HPMO can act as a smart and versatile nanoplatform with pH-responsive multimodal imaging and site-specific drug delivery for the development of accurate diagnosis and effective therapy for cancer.

SELEÇÃO DE REFERÊNCIAS
DETALHE DA PESQUISA