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1.
Carbohydr Polym ; 231: 115692, 2020 Mar 01.
Artigo em Inglês | MEDLINE | ID: mdl-31888840

RESUMO

Protein-loaded starch microspheres were prepared by water-in-water (w/w) emulsion method. The effects of the molecular weight of starch and protein used, concentration of solutes in both dispersed and continuous phases and starch to protein mass ratio on the yield, loading capacity and encapsulation efficiency were measured. These parameters were significantly higher in Bovine serum albumin (BSA)-loaded microspheres than in lysozyme-loaded microspheres. An increase in the molecular weight of starch, solute concentration in dispersed and continuous phases increased the yield. The encapsulation efficiency was significantly improved when the starch to BSA mass ratio was increased. When the starch to BSA mass ratio was 15:1, the encapsulation efficiency reached about 100 % with a loading capacity of 7.3 g/100 g. This method is more effective when both core (protein) and shell (starch) materials with high molecular weight are used. This approach is environmentally friendly and the processing parameters can be easily optimized.

2.
Int Immunopharmacol ; 79: 106155, 2020 Jan 06.
Artigo em Inglês | MEDLINE | ID: mdl-31918059

RESUMO

BACKGROUND: ALOX5, IL6R and SFTPD are all immune related genes that may be involved in the development of lung cancer. We sought to explore the effect of polymorphisms of these genes on the risk of lung cancer. METHODS: Six single nucleotide polymorphisms (SNPs) were genotyped using a MassARRAY platform in a case-control cohort including 550 patients with lung cancer and 550 healthy controls. RESULTS: The rs4845626-T and rs4329505-C alleles were associated with a decreased risk of lung cancer (p < 0.001), while the rs745986-G and rs2245121-A alleles were correlated with an increased risk of lung cancer (p < 0.01). The rs4845626-GT/GG and rs4329505-TC genotypes were protective against lung cancer (p < 0.001). However, the rs745986-AG and rs2245121-AG/AA genotypes were associated with an increased risk of lung cancer (p < 0.01). Stratification analysis showed that the rs4845626 and rs4329505 polymorphisms of IL6R were associated with a reduced risk of lung cancer in both smokers and nonsmokers (p < 0.05). However, rs892690, rs745986 and rs2115819 of ALOX5 were associated with an increased risk of disease in nonsmokers, while rs2245121 of SFTPD was correlated with a higher risk of disease in smokers (p < 0.05). CONCLUSION: Our results provide candidate SNPs for early screening for lung cancer and new clues for further study of the pathogenesis of the disease.

3.
Mol Med Rep ; 2020 Jan 09.
Artigo em Inglês | MEDLINE | ID: mdl-31922242

RESUMO

Increasing evidence has indicated that miR­155 is closely associated with apoptosis, which may protect the myocardium and diminish the infarct area in myocardial ischemia reperfusion injury (IRI). In addition, studies have revealed that miR­155 serves a leading role in promoting fibroblast inflammation, cardiac dysfunction and other aspects of myocardial injury. The present study aimed to uncover the function and potential biological mechanism of miR­155 in myocardial IRI. The rat H9c2 myocardial cells was treated with hypoxia/reoxygenation (H/R) to simulate IRI in vitro. Reverse transcription­quantitative polymerase chain reaction (RT­qPCR) was used to detect the expression levels of miR­155 mRNA. Cell Counting Kit­8 and flow cytometry assays and western blot analysis were applied to determine the biological behaviors of the H/R­treated cells. The association between miR­155 and BAG family molecular chaperone regulator 5 (BAG5) was predicted by bioinformatics software and was confirmed by dual luciferase assay. RT­qPCR and western blot analysis were used to analyze the expression of BAG5. The key proteins involved in mitogen­activated protein kinase (MAPK)/JNK signaling pathway were detected by western blot analysis. The data from the RT­qPCR assay indicated that the expression of miR­155 was markedly upregulated in the H/R model, and that downregulation of miR­155 may promote cell proliferation and inhibit cell apoptosis, and vice versa. BAG5, which was downregulated in the H/R model, was confirmed as a target of miR­155 and negatively modulated by miR­155. The key proteins involved in MAPK/JNK signaling, which were highly expressed in the H/R model, were suppressed by treatment with the miR­155 inhibitor, and overexpression of BAG5 promoted the protective effect of miR­155 inhibition on cell injury caused by H/R. In addition, the expression patterns of hypoxia­inducible factor 1­α and von Hippel­Lindau were altered following different treatments. Taken together, the data from the present study indicated that miR­155 inhibition represented a potential treatment strategy to improve myocardial H/R injury, which may be associated with targeting BAG5 and inhibition of the MAPK/JNK pathway.

4.
Environ Health Perspect ; 128(1): 17006, 2020 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-31909654

RESUMO

BACKGROUND: Both extreme heat and air pollution exposure during pregnancy have been associated with preterm birth; however, their combined effects are unclear. OBJECTIVES: Our goal was to estimate the independent and joint effects of heatwaves and fine particulate matter [PM <2.5µm in aerodynamic diameter (PM2.5)], exposure during the final gestational week on preterm birth. METHODS: Using birth registry data from Guangzhou, China, we included 215,059 singleton live births in the warm season (1 May-31 October) between January 2015 and July 2017. Daily meteorological variables from 5 monitoring stations and PM2.5 concentrations from 11 sites were used to estimate district-specific exposures. A series of cut off temperature thresholds and durations (2, 3, and 4 consecutive d) were used to define 15 different heatwaves. Cox proportional hazard models were used to estimate the effects of heatwaves and PM2.5 exposures during the final week on preterm birth, and departures from additive joint effects were assessed using the relative excess risk due to interaction (RERI). RESULTS: Numbers of preterm births increased in association with heatwave exposures during the final gestational week. Depending on the heatwave definition used, hazard ratios (HRs) ranged from 1.10 (95% CI: 1.01, 1.20) to 1.92 (1.39, 2.64). Associations were stronger for more intense heatwaves. Combined effects of PM2.5 exposures and heatwaves appeared to be synergistic (RERIs>0) for less extreme heatwaves (i.e., shorter or with relatively low temperature thresholds) but were less than additive (RERIs<0) for more intense heatwaves. CONCLUSIONS: Our research strengthens the evidence that exposure to heatwaves during the final gestational week can independently trigger preterm birth. Moderate heatwaves may also act synergistically with PM2.5 exposure to increase risk of preterm birth, which adds new evidence to the current understanding of combined effects of air pollution and meteorological variables on adverse birth outcomes. https://doi.org/10.1289/EHP5117.

5.
Artigo em Inglês | MEDLINE | ID: mdl-31903502

RESUMO

The article 'Addition of glyceryl monostearate affects the crystallization behavior and polymorphism of palm stearin', written by Liyan Liu, Lin Li, Liting Wan, Linlu Mao, Bing Li, Xia Zhang, was originally published electronically on the publisher's internet portal (currently SpringerLink) on 14 December 2019 with open access.

6.
BMC Cancer ; 20(1): 10, 2020 Jan 06.
Artigo em Inglês | MEDLINE | ID: mdl-31906882

RESUMO

BACKGROUND: Surgery is the only way to cure gastric adenocarcinoma (GAC), and chemotherapy is the basic adjuvant management for GAC. A significant prognostic nomogram for predicting the respective disease-specific survival (DSS) rates of GAC patients who receive surgery and chemotherapy has not been established. OBJECTIVE: We were planning to establish a survival nomogram model for GAC patients who receive surgery and chemotherapy. METHODS: We identified 5764 GAC patients who had received surgery and chemotherapy from the record of Surveillance, Epidemiology, and End Results (SEER) database. About 70% (n = 4034) of the chosen GAC patients were randomly assigned to the training set, and the rest of the included ones (n = 1729) were assigned to the external validation set. A prognostic nomogram was constructed by the training set and the predictive accuracy of it was validated by the validation set. RESULTS: Based on the outcome of a multivariate analysis of candidate factors, a nomogram was developed that encompassed age at diagnosis, number of regional lymph nodes examined after surgery, number of positive regional lymph nodes, sex, race, grade, derived AJCC stage, summary stage, and radiotherapy status. The C-index (Harrell's concordance index) of the nomogram model was some larger than that of the traditional seventh AJCC staging system (0.707 vs 0.661). Calibration plots of the constructed nomogram displayed that the probability of DSS commendably accord with the survival rate. Integrated discrimination improvement (IDI) revealed obvious increase and categorical net reclassification improvement (NRI) showed visible enhancement. IDI for 3-, 5- and 10- year DSS were 0.058, 0.059 and 0.058, respectively (P > 0.05), and NRI for 3-, 5- and 10- year DSS were 0.380 (95% CI = 0.316-0.470), 0.407 (95% CI = 0.350-0.505), and 0.413 (95% CI = 0.336-0.519), respectively. Decision curve analysis (DCA) proved that the constructed nomogram was preferable to the AJCC staging system. CONCLUSION: The constructed nomogram supplies more credible DSS predictions for GAC patients who receive surgery and chemotherapy in the general population. According to validation, the new nomogram will be beneficial in facilitating individualized survival predictions and useful when performing clinical decision-making for GAC patients who receive surgery and chemotherapy.

7.
Histol Histopathol ; : 18198, 2020 Jan 09.
Artigo em Inglês | MEDLINE | ID: mdl-31916583

RESUMO

BACKGROUND: Studies have shown that estrogen can protect the function of the sublingual gland, but the specific mechanism is still unclear. Besides, the STIM1/Orai1 pathway is important to secretion in the salivary gland. Here, we explore the possible effects of estrogen on sublingual gland function by observing changes of STIM1 and Orai1 levels in the sublingual glands of ovariectomized rats. METHODS: 42 adult female Sprague-Dawley rats were randomly divided into three groups: SHAM, OVX, and OVX+E (n = 14 per group). Two weeks after ovariectomy, rats were treated with estrogen (ß-estradiol). The expression of STIM1 and Orai1 in the sublingual gland were observed by double label-immunohistochemistry and immunofluorescence. Calcium imaging was conducted to observe changes in cellular Ca²âº levels. RESULTS: IHC and IF showed that the levels of both STIM1 and Orai1 decreased following ovariectomy, but increased to SHAM levels after estrogen treatment. By IF, STIM1 and Orai1 exhibited perfect co-localization. Calcium imaging results showed that the Ca²âº in the cells decreased after ovariectomy. Estrogen intervention returned levels of these proteins and Ca²âº to the same as those in the control group. CONCLUSION: This study demonstrates that low estrogen status significantly reduced the expression of STIM1 and Orai1 in the sublingual gland of rats, along with cellular Ca²âº levels. These data provide insight into the likely mechanisms underlying sublingual gland secretion dysfunction during menopause.

8.
Biol Trace Elem Res ; 2020 Jan 10.
Artigo em Inglês | MEDLINE | ID: mdl-31925743

RESUMO

Arsenic is identified as a known carcinogen and ubiquitously exists in nature. It appears that accumulation of inorganic arsenic (iAs) and its methylated metabolites in various tissues is closely correlated with the long-term toxicity and carcinogenicity of this metalloid. In this study, various arsenic species in murine tissues, especially in the cerebral cortex, cerebellum, and hippocampus, were determined after long-term exposure to 25, 50, 100, and 200 mg/L sodium arsenite in drinking water for 1 and 12 months. Our data showed that the amount of total arsenic (TAs) increased in an obvious dose-dependent manner in various tissues, and TAs levels were in the order of urinary bladder > brain > lung > liver > kidney > spleen. Furthermore, iAsIII and DMA could be observed in all tissues and brain regions with DMA being the predominant metabolite. The bladder, brain, and lung orderly contained the higher levels of DMA, while the liver, kidney, and spleen accumulated the higher proportion of iAsIII. MMA was preferentially accumulated in the lung and bladder of mice regardless of arsenic exposure doses or duration. What's more, amazingly higher levels of MMA were observed in the hippocampus, which was distinguished from the cerebral cortex and cerebellum. Together with these results, our study clearly demonstrates that the accumulation of iAs and its methylated metabolites is tissue-specific and even not homogeneous among different brain regions in mice by long-term exposure to arsenite. Our study thus provides crucial information for recognizing arsenical neurotoxicity, and reducing the uncertainty in the risk assessment for this toxic metalloid.

9.
Sci Total Environ ; 704: 135305, 2020 Feb 20.
Artigo em Inglês | MEDLINE | ID: mdl-31896219

RESUMO

Phosphorus is vital for living creatures and will run out in the next few hundred years. The imbalanced phosphate rock distribution and inefficient consumption make phosphorus management of great importance. As China has an undeniable influence on global phosphorus production and consumption, understanding its changing historical patterns is critical for phosphorus resource management and water quality improvement. However, most existing research focus on anthropogenic phosphorus flows in the agricultural sector for a specific year, making the evaluation of such changes difficult. Therefore, substance flow analysis and principal component analysis for phosphorus flows between 1980 and 2015 were performed to understand phosphorus consumption structure change and the build-up of legacy phosphorus in China. The results show that although China's phosphorus utilization efficiency has decreased over time, it is still higher than in most other countries. The research also demonstrates the effectiveness of combining multiyear substance flow analysis and principal component analysis to improve the transparency of identifying underlying consumption structure change during resource management.

10.
J Sci Food Agric ; 100(2): 607-613, 2020 Jan 30.
Artigo em Inglês | MEDLINE | ID: mdl-31591730

RESUMO

BACKGROUND: α-Dicarbonyl compounds are widely generated in the Maillard reaction, caramelization and oil oxidation during heat treatment. These compounds can readily react with lysine and arginine residues of a protein, whereas the influence of these compounds on protein structure and quality has seldom been revealed. This study compared influence of glycation by glucose and α-dicarbonyl compounds on amyloid-like aggregation of ß-lactoglobulin (ß-LG), both fibrillation kinetics and conformation of aggregates were studied. RESULTS: Compared with glycation by glucose, the glycation by α-dicarbonyl compounds resulted in faster reduction of free amino group, sulfydryl group, and the relative content of ß-sheet secondary structure, according to the ultraviolet (UV) spectra or circular dichroism (CD) spectra results. Based on the analysis of fibrillation kinetics using thioflavin T (ThT) binding assay, the glycation by α-dicarbonyls were more efficient in suppressing the growth of fibrillar aggregates. In addition, glycation by α-dicarbonyl resulted in amorphous oligomers, which were compared with the amyloid-like aggregates in control and glucose-glycated samples, based on the transmission electron microscopy (TEM) observation. CONCLUSIONS: Glycation by α-dicarbonyl compounds induced larger decline in the ß-sheet structure of ß-LG than glycation by glucose, and thus largely suppressed the amyloid-like aggregation of ß-LG and changed the morphology of aggregates. © 2019 Society of Chemical Industry.


Assuntos
Amiloide/química , Lactoglobulinas/química , Animais , Bovinos , Dicroísmo Circular , Glucose/química , Glicosilação , Temperatura Alta , Agregados Proteicos , Estrutura Secundária de Proteína
11.
Food Chem ; 306: 125563, 2020 Feb 15.
Artigo em Inglês | MEDLINE | ID: mdl-31606626

RESUMO

The storage stability of two kinds of interesterified blend-based fast-frozen special fats (PS:SO-IBSF, PS:RO-IBSF) with varied triacylglycerols (TAGs) compositions under different temperatures for 4 weeks was investigated. Rancimat and peroxide values experiments indicated that both IBSFs display good oxidation stability throughout a 4-week storage. As for the physical properties of both IBSFs, the solid fat content and hardness decreased with the increase of storage temperature, and IBSFs still exhibited a viscoelastic solid-like behavior. X-ray diffraction results showed that crystal transformation from ß'- to ß-form was more serious when stored at 25 °C. The more content of ECN 50-type TAGs in PS:RO-IBSF is helpful to reduce its crystal transformation from ß'-to ß-form compared to PS:SO-IBSF. On the other hand, storage at 4 °C was beneficial for both IBSFs to keep their crystal network integrity, and the PS:RO-IBSF maintained better quality under the same storage conditions.


Assuntos
Gorduras/química , Cristalização , Esterificação , Congelamento , Oxirredução , Estresse Oxidativo , Temperatura Ambiente , Difração de Raios X
12.
Lancet Neurol ; 2019 Dec 09.
Artigo em Inglês | MEDLINE | ID: mdl-31831388

RESUMO

BACKGROUND: Previous randomised trials have shown an overwhelming benefit of mechanical thrombectomy for treating patients with stroke caused by large vessel occlusion of the anterior circulation. Whether endovascular treatment is beneficial for vertebrobasilar artery occlusion remains unknown. In this study, we aimed to investigate the safety and efficacy of endovascular treatment of acute strokes due to vertebrobasilar artery occlusion. METHODS: We did a multicentre, randomised, open-label trial, with blinded outcome assessment of thrombectomy in patients presenting within 8 h of vertebrobasilar occlusion at 28 centres in China. Patients were randomly assigned (1:1) to endovascular therapy plus standard medical therapy (intervention group) or standard medical therapy alone (control group). The randomisation sequence was computer-generated and stratified by participating centres. Allocation concealment was implemented by use of sealed envelopes. The primary outcome was a modified Rankin scale (mRS) score of 3 or lower (indicating ability to walk unassisted) at 90 days, assessed on an intention-to-treat basis. The primary safety outcome was mortality at 90 days. Secondary safety endpoints included the rates of symptomatic intracranial haemorrhage, device-related complications, and other severe adverse events. The BEST trial is registered with ClinicalTrials.gov, NCT02441556. FINDINGS: Between April 27, 2015, and Sept 27, 2017, we assessed 288 patients for eligibility. The trial was terminated early after 131 patients had been randomly assigned (66 patients to the intervention group and 65 to the control group) because of high crossover rate and poor recruitment. In the intention-to-treat analysis, there was no evidence of a difference in the proportion of participants with mRS 0-3 at 90 days according to treatment (28 [42%] of 66 patients in the intervention group vs 21 [32%] of 65 in the control group; adjusted odds ratio [OR] 1·74, 95% CI 0·81-3·74). Secondary prespecified analyses of the primary outcome, done to assess the effect of crossovers, showed higher rates of mRS 0-3 at 90 days in patients who actually received the intervention compared with those who received standard medical therapy alone in both per-protocol (28 [44%] of 63 patients with intervention vs 13 [25%] of 51 with standard therapy; adjusted OR 2·90, 95% CI 1·20-7·03) and as-treated (36 [47%] of 77 patients with intervention vs 13 [24%] of 54 with standard therapy; 3·02, 1·31-7·00) populations. The 90-day mortality was similar between groups (22 [33%] of 66 patients in the intervention vs 25 [38%] of 65 in the control group; p=0·54) despite a numerically higher prevalence of symptomatic intracranial haemorrhage in the intervention group. INTERPRETATION: There was no evidence of a difference in favourable outcomes of patients receiving endovascular therapy compared with those receiving standard medical therapy alone. Results might have been confounded by loss of equipoise over the course of the trial, resulting in poor adherence to the assigned study treatment and a reduced sample size due to the early termination of the study. FUNDING: Jiangsu Provincial Special Program of Medical Science.

13.
Artigo em Inglês | MEDLINE | ID: mdl-31838608

RESUMO

Low crystallization-rate and formation of crystalline clusters makes palm stearin unpopular in fat-based products especially in their post-processing stage. Addition of emulsifiers is commonly used to overcome these drawbacks, since they are believed to induce or stabilize specific polymorphs of palm stearin. Glyceryl monostearate (GMS) was applied in palm stearin (1%, 2%, and 4% w/w) in this study, and the mechanisms on crystallization of palm stearin were investigated by means of differential scanning calorimetry (DSC), X-ray diffraction (XRD), and polarized light microscopic (PLM) method. Data showed that GMS prompted the isothermal crystallization (15-30 °C) in a dose-dependent manner. Crystallization turned to low super-cooling sporadic nucleation at 30 °C. Besides, GMS led to an earlier onset of crystallization during cooling. GMS-palm stearin blends crystallized to form α polymorphs at first and subsequently underwent polymorphic transition to become ß' polymorphs. Addition of 4% w/w GMS in palm stearin significantly decreased the size of crystals, which is helpful to reduce the grainy mouth feel of fat products in practice.

14.
Ying Yong Sheng Tai Xue Bao ; 30(12): 4143-4149, 2019 Dec.
Artigo em Chinês | MEDLINE | ID: mdl-31840459

RESUMO

Investigation of stable isotope composition under different land use types is helpful for understanding soil water movement and hydrological effects of land use change. We collected soil samples in profiles > 15 m deep under four land use types (i.e. farmland, grassland, Salix cheilophila and Populus sp.) in the loess deposits of northern Shaanxi. We measured hydrogen and oxygen stable isotope composition of soil water to explore the mechanism of soil water movement and the impacts of land use types. The isotope compositions of soil water under four land use types were significantly different. The δD values of soil water under farmland, grassland, S. cheilophila and Populus sp. were -81.1‰--60.1‰, -91.2‰--61.0‰, -87.4‰--63.6‰ and -73.5‰--62.2‰, while the δ18O values were -11.2‰--7.6‰, -12.6‰--8.2‰, -11.5‰--8.1‰ and -9.9‰--7.7‰, respectively. The soil water stable isotopes fluctuated across the profiles. The soil water isotope compositions in the layers of 0-3 m changed sharply, with the δD values being -80.2‰--61.8‰, -75.9‰--65.5‰, -76.0‰--63.6‰ and -73.5‰--62.2‰, respectively. In the layers of 3-12 m, the isotope profiles of farmland and grassland were parabolic, whereas those of S. cheilophila and Populus sp. were relatively stable. Soil water isotope compositions in the layers deeper than 12 m were generally stable with the δD values of -80.8‰--71.5‰, -83.0‰--67.5‰, -87.4‰--76.0‰ and -67.5‰--64.3‰, respectively. Across the four land use types, soil water stable isotope compositions were not significantly different either in the shallow layers or in the deep soil layers, but their differences in the layers of 3-12 m were significant. Soil moisture was mainly recharged from precipitation with piston flow as the main form of soil water movement. Soil water under four land use types might be recharged by wet events of different intensities. Soil water under farmland and grassland could be recharged by wet events of small intensity, but that under S. cheilophila and Populus sp. may be mainly recharged by the rainstorm in summer and autumn.

15.
Sci Total Environ ; : 135273, 2019 Nov 24.
Artigo em Inglês | MEDLINE | ID: mdl-31806321

RESUMO

The widespread use of nanomaterials has raised concerns about the potential impact of nanoparticles on human health and the natural environment. Although high doses of TiO2 nanoparticles (NPs) are toxic to animals, low doses of TiO2 NPs have been shown to benefit their growth and immune functions. Intestinal microorganisms are critical in maintaining the normal life activities and ensuring the health of their host. The intestinal microorganisms of lepidopteran insects can promote growth and development, foster insecticide resistance, and improve resilience against diseases. However, to date, there is no report on the effects of TiO2 NPs on the intestinal microbiota of lepidopteran insects. In this work, we examined the effects of a low dose of TiO2 NPs (5 mg/L) on the intestinal microbiota of silkworm (Bombyx mori). The results showed that the exposure to TiO2 NPs did not alter the dominant species of intestinal microbiota significantly, but changed the abundance of individual species of intestinal microorganisms. Specifically, exposure to TiO2 NPs increased the uniformity of intestinal microorganisms. The abundance of Lachnospiraceae_NK4A136_group, involved in the metabolism of nutrients, as well as the abundance of Pseudomonas and Sphingomonas, both involved in detoxification and disease resistance, was increased. Meanwhile, among the non-dominant species, the conditional pathogenic bacteria Serratia exhibited decreased abundance. In addition, exposure to TiO2 NPs also increased the abundance of norank_f_Bacteroidales_S24-7_group, which could help relieve inflammation and regulate immune functions. The current study is the first to report the effects of TiO2 NPs on the intestinal microbiota of lepidopteran insects. The results demonstrated that TiO2 NPs could alter the composition of the intestinal microbiota of B. mori, and thus promote its growth and development, regulate its immune functions, and enhance its resistance to insecticide.

16.
Environ Pollut ; : 113666, 2019 Nov 23.
Artigo em Inglês | MEDLINE | ID: mdl-31806462

RESUMO

Fluoxastrobin is one of the most widely used strobilurin fungicides, however, application of the fungicides may result in soil residues leading to environmental damage including oxidative stress and damage to sentinel organisms (i.e. earthworms). While this has been demonstrated in artificial soil, the biochemical response of Eisenia fetida exposed to fluoxastrobin in natural soils is unclear. This study utilized three typical natural soils (fluvo-aquic soils, red clay, and black soils) to evaluate the biochemical response of Eisenia fetida exposed to fluoxastrobin (0.1, 1.0, 2.5 mg/kg) including the production of reactive oxygen species, impact on three enzyme activities, lipid peroxidation, and 8-hydroxydeoxyguanosine after a 4-week exposure. The effects of fluoxastrobin on Eisenia fetida in different soils were assessed using an integrated biomarker response (IBR). The findings may be possible to state that the toxic effects of fluoxastrobin in artificial cannot exactly represent that in natural soils. Specifically, the fluoxastrobin subchronic toxicity was highest in red clay and lowest in black soil among the three natural soils. Furthermore, the 8-OHdG content was more sensitive to fluoxastrobin in all six environmental indicators of the present study.

17.
Nat Chem ; 2019 Dec 02.
Artigo em Inglês | MEDLINE | ID: mdl-31792388

RESUMO

Topological transitions between considerably different phases typically require harsh conditions to collectively break chemical bonds and overcome the stress caused to the original structure by altering its correlated bond environment. In this work we present a case system that can achieve rapid rearrangement of the whole lattice of a metal-organic framework through a domino alteration of the bond connectivity under mild conditions. The system transforms from a disordered metal-organic framework with low porosity to a highly porous and crystalline isomer within 40 s following activation (solvent exchange and desolvation), resulting in a substantial increase in surface area from 725 to 2,749 m2 g-1. Spectroscopic measurements show that this counter-intuitive lattice rearrangement involves a metastable intermediate that results from solvent removal on coordinatively unsaturated metal sites. This disordered-crystalline switch between two topological distinct metal-organic frameworks is shown to be reversible over four cycles through activation and reimmersion in polar solvents.

18.
Int J Biol Macromol ; 2019 Dec 09.
Artigo em Inglês | MEDLINE | ID: mdl-31830451

RESUMO

In this study, label-free quantitative proteomics was used to investigate the biological functions of M. oleifera seed proteins, which resulted in the identification of milk-clotting proteases. In total, 921 proteins were identified, and proteins within the molecular weight range of 30-50 kDa were abundant. The identified proteins were mainly involved in catalytic activity and metabolic processes associated with carbohydrate and protein metabolism, among which, proteases in the observed molecular weight range could possibly be responsible for the previously reported milk-clotting activity. An aspartic-type endopeptidase with molecular mass of 45,517 Da was purified from M. oleifera seeds using ammonium sulfate precipitation, ultrafiltration, and preparative high performance liquid chromatography, and was characterized using liquid chromatography-mass spectrometry (LC-MS)/MS. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis revealed that the purified protease exhibited hydrolase activity and was involved in several metabolic pathways, which further confirmed that proteomic analysis can assist in the purification of the milk-clotting protease. The optimal temperature and pH required for protease activity were 60 °C and 5.0, respectively. The high thermal stability and good pH stability of the protease indicated that it can be used in the dairy industry.

19.
Onco Targets Ther ; 12: 9873-9885, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31819485

RESUMO

Background: Previous studies have shown that P73 antisense RNA 1T (non-protein coding), also known as TP73-AS1, is a long non-coding RNA (lncRNA) and involved in the development of medulloblastoma. However, the regulatory mechanism of lncRNA TP73-AS1 in medulloblastoma was still unclear, the present study was aimed to investigate the detailed functions and the mechanism of TP73-AS1 in regulation of medulloblastoma. Materials and methods: The levels of TP73-AS1, miR-494-3p, and Eukaryotic initiation factor 5A2 (EIF5A2) were determined using quantitative real-time PCR (qRT-PCR), in situ hybridization (ISH), or Immunohistochemistry (IHC). The function of TP73-AS1 in proliferation, apoptosis, migration, and invasion of medulloblastoma cells was evaluated using cell counting Kit-8 (CCK-8), flow cytometry, and transwell assay, respectively. The protein levels were determined by Western blot. Bioinformatics analysis and dual-luciferase reporter assay, RNA immunoprecipitation (RIP) and pull-down assay were used to search and confirm the target gene of TP73-AS1 and miR-494-3p. The effect of TP73-AS1 knockdown in vivo was detected by animal experiment. Results: The levels of TP73-AS1 and EIF5A2 were up-regulated, while miR-494-3p expression was down-regulated in medulloblastoma tissues and cells, ELF5A2 was a direct target of miR-494-3p, and miR-494-3p bound to TP73-AS1. The knockdown of TP73-AS1 inhibited cell proliferation, invasion, migration, and promoted apoptosis of medulloblastoma cells, while the miR-494-3p inhibitor abolished the effects of TP73-AS1 knockdown on medulloblastoma cells. Conclusion: TP73-AS1 positively regulated EIF5A2 expression by sponging miR-494-3p. These findings suggested that TP73-AS1 served as an oncogene and promoted the progression of medulloblastoma.

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