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1.
ACS Omega ; 7(38): 34608-34620, 2022 Sep 27.
Artigo em Inglês | MEDLINE | ID: mdl-36188246

RESUMO

Pyroptosis is an important factor affecting the proliferation, invasion, and metastasis of tumor cells. However, in multiple myeloma (MM), there are few studies on whether the occurrence of pyroptosis is related to the occurrence and prognosis of the disease. Based on the Gene Expression Omnibus and Cancer Genome Atlas database search dataset, this study identified pyroptosis-related genes with a specific prognosis, constructed and verified the prediction model by stepwise Cox regression analysis and time receiver operating characteristic curve analysis, and predicted specific functions by single-sample gene set enrichment analysis and the Kyoto Encyclopedia of Genes and Genomes. Dataset analysis identified key genes, which were used to construct a risk scoring system for the prognosis of MM. The entire test set and external verification set verified the results. The expression levels of related genes in the clinical samples were detected using fluorescence quantitative PCR. A prognostic gene model based on six pyroptosis-related genes (CYCS, NLRP9, AIM2, NOD2, CHMP3, and GSDME) was constructed. The model has an excellent prognostic ability and can be popularized in the external validation set. The predictive prognostic nomogram integrating clinical information can effectively evaluate the risk score of each patient and predict their survival. After sample validation, our study found three potential key pyroptosis-related genes in multiple myeloma. GSDME, NOD2, and CHMP3 were significantly different between MM and healthy subjects, suggesting that they are pyroptosis-related protective genes. This study shows that the key pyroptosis-related gene in the model can be used as a marker for predicting the prognosis of myeloma, which may provide a basis for clinical individualized stratification therapy.

2.
Artigo em Inglês | MEDLINE | ID: mdl-36109837

RESUMO

Doping main-group elements has become a unique strategy to fine-tune optoelectronic properties of organic 𝜋 -conjugated molecules. Herein, we report the first example of diazaphosphinine based polycyclic hydrocarbons (DP-PAHs) via facile synthetic protocols. Synthesis of diazaphosphinine heterocycles harnesses mild and efficient P - N chemistry. The heterocycles readily undergo photocyclizations by using only oxidants. The photocyclization protocol is applicable to the heterocycles with various P-centers. We successfully extended the protocols in extended DP-PAHs with 13-fused aromatic rings. Further adjusting P-chemistry allows to efficiently fine-tune the singlet and triplet emission characteristics. More importantly, single-molecule white-light-emitting character was achieved via the balanced singlet and triplet emission of DP-PAH. Therefore, this work opens up new horizons in the area of organic 𝜋 -conjugated materials.

3.
Chemistry ; : e202201812, 2022 Sep 01.
Artigo em Inglês | MEDLINE | ID: mdl-36050897

RESUMO

Solar energy-driven overall water splitting (OWS) is an attractive way for generating clean and renewable green hydrogen. One key challenge is the construction of OWS systems with high solar energy conversion efficiencies, in which how to manipulate photoexcited charge carriers to efficiently participate in the reaction is the top priority. In recent years, bismuth vanadate (BiVO4 ) has emerged as one of the most promising materials for photo(electro)catalytic OWS and considerable progress has been achieved. In this review, recent advances of BiVO4 -constituted OWS systems in both photoelectrocatalytic and photocatalytic approaches are presented in a mainline of effective charge carrier utilization. Various strategies for improved charge carrier utilization, including band structure engineering, improving charge separation, reducing charge recombination, and accelerating reaction kinetics, are summarized and analyzed in detail. Finally, perspective and outlook on further exploring the application potential of BiVO4 are proposed.

4.
Polymers (Basel) ; 14(17)2022 Sep 04.
Artigo em Inglês | MEDLINE | ID: mdl-36080746

RESUMO

Lignocellulosic biomass as abundant, renewable, and sustainable carbon feedstock is an alternative to relieve the dependence on fossil fuels and satisfy the demands of chemicals and materials. Conversions of lignocellulosic biomass to high-value-added chemicals have drawn much attention recently due to the high availability of sustainable ways. This minireview surveys the recent trends in lignocellulosic biomass conversion into furan derivatives based on the following systems: (1) ionic liquids, (2) deep eutectic solvents, and (3) biphasic systems. Moreover, the current challenges and future perspectives in the development of efficient routes for lignocellulosic biomass conversion are provided.

6.
J Colloid Interface Sci ; 629(Pt B): 598-609, 2022 Sep 22.
Artigo em Inglês | MEDLINE | ID: mdl-36179579

RESUMO

For photoelectrochemical (PEC) water splitting, constructing heterojunctions and loading co-catalysts are effective means to realizing sufficient light absorption, effective photogenerated carrier separation and fast charge transport. However, during implementation, the PEC performance of the catalyst is affected by both parasitic light absorption and reflection and the change in energy band structure due to the creation of new interfaces. Herein, in order to minimize the effect of recombination of photogenerated electron-hole pairs on the catalyst PEC performance due to the nascent interface arising from the co-catalyst compounding, WO3 and Ni/Co co-doped LaFeO3 (LFO) are constructed as heterojunctions, in which NiCo-LFO acts both as a part of the heterojunction to enhance photogenerated carrier separation and a co-catalyst to enhance the conductivity and modulate the surface state density at the catalyst-electrolyte interface. The current density of NiCo-LFO/WO3 reaches 3.92 mA cm-2, which is more than 7 times that of LFO/WO3. This work provides a reference for the efficient water splitting of B-site doped, especially the co-doped perovskite oxide as multifunctional roles integrated with conventional photoelectrodes.

7.
Int Ophthalmol ; 2022 Sep 20.
Artigo em Inglês | MEDLINE | ID: mdl-36125586

RESUMO

PURPOSE: To compare binocular static visual acuity (SVA), stereopsis, contrast sensitivity (CS) and dynamic visual acuity (DVA) of 5 combinations of bifocal intraocular lenses (IOLs), trifocal IOLs and extended-depth-of-focus (EDOF) IOLs in age-related cataract patients. METHODS: Two hundred and ninety-two eyes of 146 patients who underwent cataract surgery in the ophthalmology department of the First Affiliated Hospital of Chongqing Medical University were involved. Subgroups included group MM (33patients, bilaterally bifocal IOL, ZMB00), group TT (31patients, bilaterally trifocal IOL, AT LISA tri839MP), group XX (34patients, bilaterally EDOF IOL, ZXR00), group MX (25patients, bifocal IOL, ZMB00 + EDOF IOL, ZXR00) and group TX (23patients, trifocal IOL, AT LISA tri839MP + EDOF IOL, ZXR00). The uncorrected SVAs (UDVA, UIVA and UNVA), uncorrected DVAs (UDDVA, UIDVA and UNDVA), near and distance stereopsis, and CS were assessed 3 months postoperatively. RESULTS: Subgroups of TT, XX, MX and TX showed better UIVA than MM (bP = 0.039, 0.021, 0.035 and 0.037, respectively). MX showed better UNVA than MM and TX (bP = 0.031 and 0.013, respectively). MX group had the optimal outcomes of both near and distance stereopsis. In the UDDVA, XX group and MX group showed better outcomes than TX group at 24 fps (frames per second) (bP = 0.019 and 0.023, respectively). XX group and MX group showed optimal outcomes at all speeds of UIDVA (P = 0.001, 0.005, 0.003 and 0.005, respectively). As the speed increased, the XX group and the MX group showed better UNDVA than the MM group and the TT group (P = 0.019, 0.002 and 0.003, respectively). CONCLUSIONS: Mix-and-match implantation of bifocal IOLs and EDOF IOLs provides excellent and stable binocular visual outcomes including SVA, stereopsis and DVA in distant and near distances.

8.
Int J Biol Macromol ; 220: 1212-1220, 2022 Aug 29.
Artigo em Inglês | MEDLINE | ID: mdl-36049566

RESUMO

The peptidoglycan recognition protein (PGRP), an important pattern recognition receptor of insects, is significant for reducing innate immunity and effective pest control. We cloned four PGRP genes (LsPGRP-LB, LsPGRP-LB1, LsPGRP-LE, and LsPGRP-SC2) from the cigarette beetle, Lasioderma serricorne (Fabricius), which encoded proteins of 216, 197, 317, and 190 amino acids, respectively. Three LsPGRPs were predominantly expressed in the larval and pupal stages, whereas LsPGRP-LE displayed high expression in adults. All the four LsPGRPs genes were highly expressed in the midgut and integument. Pathogen inoculation revealed that the four LsPGRPs actively responded to Escherichia coli and its peptidoglycan. The transcription levels of LsPGRP-LE and LsPGRP-SC2 increased significantly after Staphylococcus aureus stimulation. RNA interference-mediated knockdown of the four LsPGRPs led to increased larval mortality when challenged by E. coli, and the expression of four antimicrobial peptide genes (LsCole, LsAtt2, LsDef1 and LsDef2) had a significant decrease. Higher mortality and lower AMP expression were also observed in L. serricorne under S. aureus infection after silencing LsPGRP-LE and LsPGRP-SC2. Our results suggest that the four LsPGRP genes play important and distinct regulatory roles in the antibacterial defense response of L. serricorne.

9.
J Am Chem Soc ; 144(38): 17540-17548, 2022 Sep 28.
Artigo em Inglês | MEDLINE | ID: mdl-36103140

RESUMO

A critical bottleneck for realizing an efficient Schottky type Si photoelectrode is minimizing the charge extraction losses across the heterointerface via reducing the unfavorite defects. This requires a clear microscopic insight into the correlation between interfacial features and photoconversion. Herein, by taking the n-Si/oxide (MOx)/Ni as the prototype, the heterointerface with the different characteristics and its effects on charge transportation and the corresponding photoelectric/photoelectrochemical (PEC) behaviors were clarified. An ultra-thin AlOx layer can effectively diminish the interfacial pinning of n-Si/Ni and significantly facilitate the photoconversion; meanwhile, it results in some unexpected donor-like deep defects at around 0.59 eV below the conduction band of n-Si, which could be ionized under a reverse bias and cause about 10% photogenerated charge recombination. Fortunately, these deep defects can be further eliminated by cooperating AlOx with a thin Au layer. The AlOx/Au dual-interlayer can remove almost all unexpected defects and maximize the efficiency of the electric field for charge extraction from semiconductor Si for the surface catalytic reaction. Eventually, the n-Si/SiOx/AlOx/Au/Ni/NiFeOx photoanode exhibited a record fill factor of 0.75 for the corresponding photoelectric device and an applied bias photon-to-current efficiency of 3.71% for PEC water oxidation. This study provides definite insights into interfacial electronic states and elaborates their crucial role in solar photoelectric conversion.

10.
World J Clin Cases ; 10(21): 7438-7444, 2022 Jul 26.
Artigo em Inglês | MEDLINE | ID: mdl-36158020

RESUMO

BACKGROUND: Deep Sylvian meningiomas are rare and difficult to diagnose when small tumours lead to various symptoms. The difficulty associated with surgery is underestimated. Our case involved a mass (11 mm × 12 mm × 12 mm in size) in the right Sylvian fissure. It is the smallest deep Sylvian meningioma known and might be more easily misdiagnosed than previous examples. CASE SUMMARY: A well-enhanced mass in the right Sylvian fissure of a 26-year-old male with a three-month history of seizure was identified via magnetic resonance imaging. The patient underwent operations twice for seizure control. During the first operation, the tumour was surrounded by the second segment of the middle cerebral artery and its numerous perforators. Partial resection had to be selected due to mild arterial damage. After the first operation, the patient presented with simple partial seizure. During reoperation, we isolated the anatomical structure near the tumour and the tumour over and removed it from its dorsal side by piecemeal resection. CONCLUSION: This case reported the smallest deep Sylvian meningioma according to a literature review. Preoperative diagnosis is a crucial step due to deep Sylvian meningioma firmly adhering to the middle cerebral artery and its perforators. Adequate preparation is crucial to ensure the success of surgery.

11.
Arch Gynecol Obstet ; 2022 Aug 25.
Artigo em Inglês | MEDLINE | ID: mdl-36006486

RESUMO

PURPOSE: The study aims to investigate the relationship between endometrial thickening and endometrial lesions in postmenopausal women. METHODS: Totally 390 postmenopausal patients with endometrial thickening ≥ 5 mm were enrolled from June 2016 to April 2020, among whom 188 patients were asymptomatic and 202 patients were symptomatic. RESULTS: There were 50 cases with endometrial cancer and precancerous lesions and 150 cases with benign lesions in the symptomatic group, significantly higher than that in the asymptomatic group. The most common pathological type in the asymptomatic group was endometrial polyp. In the asymptomatic group, statistically significant differences were found in endometrial thickness between patients with endometrial cancer and precancerous lesion (group B) and those with benign lesions and non-organic lesions (group A). Statistically significant differences were also found in age, endometrial thickness, hypertension, full-term delivery time and miscarriage times between group A and group B. Regression analysis indicated that hypertension and endometrial thickness were independent risk factors for endometrial cancer and precancerous lesions in the symptomatic group. ROC analysis showed that 10.5 mm was the optimal threshold for predicting endometrial cancer and precancerous lesions in the asymptomatic group, with sensitivity of 100% and specificity of 78.3%. CONCLUSION: The incidence of endometrial cancer and precancerous lesions in postmenopausal women with endometrial thickening and vaginal bleeding is higher than that of asymptomatic women. The endometrial thickening in postmenopausal asymptomatic women is mainly benign, and the threshold for predicting endometrial cancer and precancerous lesions is 10.5 mm.

12.
Environ Technol ; : 1-20, 2022 Aug 11.
Artigo em Inglês | MEDLINE | ID: mdl-35929884

RESUMO

Global climate issues have been gaining international attention in recent years. As the largest developing country and the prime carbon emitter, the Chinese government has proposed a strategic 'double carbon' target for carbon emissions. To predict carbon emissions more accurately, clarify the future supply situation and optimise resource allocation, based on the grey MGM(1,m,N|τ) model, we introduced and applied the particle swarm algorithm to determine the time lag parameter τ and proposed a new MGM(1,m,N|τ) grey model. We give a detailed modelling procedure, including calculation steps and intelligent optimisation algorithms, by fully considering the effect of time lag. In this study, this new model is used to simulate and forecast China's carbon emissions from 2010 to 2019 and compare it with other traditional grey models and their improved time-lagged forms. The results show that the new model has significant advantages in prediction accuracy and validity, plus good prediction performance for carbon emissions, which can be extended to more macro and micro energy consumption prediction problems.

13.
Adv Mater ; 34(37): e2203320, 2022 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-35916758

RESUMO

Titanium dioxide (TiO2 ) nanocrystals have attracted great attention in heterogeneous photocatalysis and photoelectricity fields for decades. However, contradicting conclusions on the crystallographic orientation and exposed facets of TiO2 nanocrystals frequently appear in the literature. Herein, using anatase TiO2 nanocrystals with highly exposed {001} facets as a model, the misleading conclusions that exist on anatase nanocrystals are clarified. Although TiO2 -001 nanocrystals are recognized to be dominated by {001} facets, in fact, anatase nanocrystals with both dominant {001} and {111} facets always co-exist due to the similarities in the lattice fringes and intersection angles between the two types of facets (0.38 nm and 90° in the [001] direction, 0.35 nm and 82° in the [111] direction). A paradigm for determining the crystallographic orientation and exposed facets based on transmission electron microscopy (TEM) analysis, which provides a universal methodology to nanomaterials for determining the orientation and exposed facets, is also given.

14.
J Colloid Interface Sci ; 628(Pt B): 595-604, 2022 Aug 17.
Artigo em Inglês | MEDLINE | ID: mdl-36027770

RESUMO

The widespread use of antibiotics leads to the increasing drug resistance of bacteria and poses a threat to human health. Therefore, there is an urgent need to develop new antibacterial strategies. Herein, based on the good photothermal properties of Copper sulfide (CuS) nanoparticles under near infrared (NIR) laser, we developed a NIR-Ⅱ window triple-mode synergetic antibacterial cCuS (cationic CuS) @Vancomycin (Van) nanoplatform. In the proposed nanoplatform, the positive charge on the surface makes cCuS@Van nanoplatform show better bacterial uptake and membrane damage; vancomycin induces chemical sterilization and provides a targeting effect to the nanoplatform; combined with the strong photothermal effect and deep tissue penetration at the excitation of 1064 nm laser, cCuS@Van nanoplatform can effectively kill bacterial. The photothermal conversion efficiency of the nanoplatform can reach 49.12 % and in vitro experiments show a sterilizing rate of more than 99.5 % to staphylococcus aureus (S. aureus) at the concentration of 3.0 µM, which also demonstrated the synergistic effect of cCuS@Van nanoplatform. In addition, low cytotoxicity to human cells conforms the good biocompatibility of the as-prepared cCuS@Van nanoplatform, which endows it a good application prospect in the field of antibacterial, such as wound healing and implant sterilization.

15.
Sci Transl Med ; 14(656): eabg9170, 2022 08 03.
Artigo em Inglês | MEDLINE | ID: mdl-35921475

RESUMO

Diabetes is a major public health issue because of its widely epidemic nature and lack of cure. Here, we show that pancreas-derived mesenchymal stem cells (PMSCs) are capable of regenerating exocrine pancreas when implanted into the kidney capsule of mice with streptozotocin (STZ)-induced diabetes. Mechanistically, we found that the regenerated exocrine pancreas elevated interleukin-6 (IL-6) in PMSC implants, which transiently activated tumor necrosis factor-α (TNF-α) and interferon-γ (IFN-γ) to inhibit IL-17, thereby rescuing damaged exocrine pancreas and islet ß cells. In addition, we used knockout mouse models to show that global lack of IL-6, TNF-α, or IFN-γ resulted in increased severity of STZ-induced diabetes and resistance to PMSC implantation therapy, confirming the roles of these factors in safeguarding pancreatic ß cells. Furthermore, removal of the kidney capsule PMSC implants at 28 days after implantation did not affect the PMSC-initiated therapeutic effect on diabetic mice. This study reveals a previously unknown role of exocrine pancreas regeneration in safeguarding ß cells and demonstrates a "soil-rescues-seed" strategy for type 1 diabetes therapy.


Assuntos
Diabetes Mellitus Experimental , Pâncreas Exócrino , Animais , Diabetes Mellitus Experimental/terapia , Interferon gama , Interleucina-6 , Camundongos , Pâncreas , Regeneração , Fator de Necrose Tumoral alfa
16.
Int J Biol Macromol ; 219: 876-885, 2022 Oct 31.
Artigo em Inglês | MEDLINE | ID: mdl-35963349

RESUMO

In this study, three polysaccharides (BGPs: BGPs-Z21, BGPs-Z23, and BGPs-Z31) were successively extracted from barley (Hordeum vulgare L.) grasses (BG) at different growth stages, including seedling (Z21), tillering (Z23), and stem elongation (Z31). The effects of in vitro simulated saliva-gastrointestinal digestion on the physicochemical characteristics and biological activities of BGPs were investigated and compared. Results showed that the simulated saliva-gastrointestinal digestion had considerable influences on reducing sugar content, chemical components, monosaccharide constituents, and molecular weights of BGPs but hardly affected their preliminarily structural characteristics. Moreover, the antioxidant activities of BGPs were weakened after the simulated saliva-gastrointestinal digestion, but their bile acid-binding capacities were remarkably enhanced. The digested BGPs-Z31 by gastric juice possessed better antioxidant benefit, and bile acid-binding capacity (80.33 %) than other digested products. Overall, these results indicated that BGPs obtained from BG are valuable for functional foods as promising bioactive ingredients.


Assuntos
Hordeum , Antioxidantes/farmacologia , Ácidos e Sais Biliares , Digestão , Hordeum/química , Monossacarídeos , Poaceae , Polissacarídeos/química , Polissacarídeos/farmacologia
17.
Chem Sci ; 13(30): 8797-8803, 2022 Aug 04.
Artigo em Inglês | MEDLINE | ID: mdl-35975146

RESUMO

The rational design of efficient catalysts for electrochemical water oxidation highly depends on the understanding of reaction pathways, which still remains a challenge. Herein, mononuclear and binuclear cobalt phthalocyanine (mono-CoPc and bi-CoPc) with a well-defined molecular structure are selected as model electrocatalysts to study the water oxidation mechanism. We found that bi-CoPc on a carbon support (bi-CoPc/carbon) shows an overpotential of 357 mV at 10 mA cm-2, much lower than that of mono-CoPc/carbon (>450 mV). Kinetic analysis reveals that the rate-determining step (RDS) of the oxygen evolution reaction (OER) over both electrocatalysts is a nucleophilic attack process involving a hydroxy anion (OH-). However, the substrate nucleophilically attacked by OH- for bi-CoPc is the phthalocyanine cation-radical species (CoII-Pc-Pc˙+-CoII-OH) that is formed from the oxidation of the phthalocyanine ring, while cobalt oxidized species (Pc-CoIII-OH) is involved in mono-CoPc as evidenced by the operando UV-vis spectroelectrochemistry technique. DFT calculations show that the reaction barrier for the nucleophilic attack of OH- on CoII-Pc-Pc˙+-CoII-OH is 1.67 eV, lower than that of mono-CoPc with Pc-CoIII-OH nucleophilically attacked by OH- (1.78 eV). The good agreement between the experimental and theoretical results suggests that bi-CoPc can effectively stabilize the accumulated oxidative charges in the phthalocyanine ring, and is thus bestowed with a higher OER performance.

18.
Braz J Med Biol Res ; 55: e11741, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-35976267

RESUMO

The aims of the present study were to evaluate the expression of prolyl 4-hydroxylase subunit alpha 3 (P4HA3) in adipocytes and adipose tissue and to explore its effect on obesity and type 2 diabetes mellitus (T2DM). We initially demonstrated that P4HA3 was significantly upregulated in the subcutaneous adipose tissue of obesity and T2DM patients, and its functional roles in adipocyte differentiation and insulin resistance were investigated using in vitro and in vivo models. The knockdown of P4HA3 inhibited adipocyte differentiation and improved insulin resistance in 3T3-L1 cells. In C57BL/6J db/db mice fed with a high fat diet (HFD), silencing P4HA3 significantly decreased fasting blood glucose and triglycerides (TG) levels, with concomitant decrease of body weight and adipose tissue weight. Further analysis showed that P4HA3 knockdown was correlated with the augmented IRS-1/PI3K/Akt/FoxO1 signaling pathway in the adipose and hepatic tissues of obese mice, which could improve hepatic glucose homeostasis and steatosis of mice. Together, our study suggested that the dysregulation of P4HA3 may contribute to the development of obesity and T2DM.


Assuntos
Diabetes Mellitus Tipo 2 , Resistência à Insulina , Pró-Colágeno-Prolina Dioxigenase/metabolismo , Animais , Dieta Hiperlipídica , Camundongos , Camundongos Endogâmicos C57BL , Obesidade/metabolismo , Fosfatidilinositol 3-Quinases
19.
Cell Death Dis ; 13(8): 722, 2022 Aug 18.
Artigo em Inglês | MEDLINE | ID: mdl-35982051

RESUMO

Sepsis is a life-threatening syndrome with disturbed host responses to severe infections, accounting for the majority of death in hospitalized patients. However, effective medicines are currently scant in clinics due to the poor understanding of the exact underlying mechanism. We previously found that blocking caspase-11 pathway (human orthologs caspase-4/5) is effective to rescue coagulation-induced organ dysfunction and lethality in sepsis models. Herein, we screened our existing chemical pools established in our lab using bacterial outer membrane vesicle (OMV)-challenged macrophages, and found 7-(diethylamino)-1-hydroxy-phenothiazin-3-ylidene-diethylazanium chloride (PHZ-OH), a novel phenothiazinium-based derivative, was capable of robustly dampening caspase-11-dependent pyroptosis. The in-vitro study both in physics and physiology showed that PHZ-OH targeted AP2-associated protein kinase 1 (AAK1) and thus prevented AAK1-mediated LPS internalization for caspase-11 activation. By using a series of gene-modified mice, our in-vivo study further demonstrated that administration of PHZ-OH significantly protected mice against sepsis-associated coagulation, multiple organ dysfunction, and death. Besides, PHZ-OH showed additional protection on Nlrp3-/- and Casp1-/- mice but not on Casp11-/-, Casp1/11-/-, Msr1-/-, and AAK1 inhibitor-treated mice. These results suggest the critical role of AAK1 on caspase-11 signaling and may provide a new avenue that targeting AAK1-mediated LPS internalization would be a promising therapeutic strategy for sepsis. In particular, PHZ-OH may serve as a favorable molecule and an attractive scaffold in future medicine development for efficient treatment of bacterial sepsis.


Assuntos
Lipopolissacarídeos , Prometazina/farmacologia , Sepse , Animais , Caspase 1 , Caspases/metabolismo , Humanos , Lipopolissacarídeos/metabolismo , Lipopolissacarídeos/farmacologia , Camundongos , Camundongos Endogâmicos C57BL , Proteínas Quinases , Proteínas Serina-Treonina Quinases , Piroptose , Sepse/tratamento farmacológico , Sepse/metabolismo
20.
J Fungi (Basel) ; 8(7)2022 Jun 29.
Artigo em Inglês | MEDLINE | ID: mdl-35887447

RESUMO

Aspergillus includes both plant pathogenic and beneficial fungi. Although endophytes beneficial to plants have high potential for plant growth promotion and improving stress tolerance, studies on endophytic lifestyles and endophyte-plant interactions are still limited. Here, three endophytes belonging to Aspergillus, AS31, AS33, and AS42, were isolated. They could successfully colonize rice roots and significantly improved rice growth. The genomes of strains AS31, AS33, and AS42 were sequenced and compared with other Aspergillus species covering both pathogens and endophytes. The genomes of AS31, AS33, and AS42 were 36.8, 34.8, and 35.3 Mb, respectively. The endophytic genomes had more genes encoding carbohydrate-active enzymes (CAZymes) and small secreted proteins (SSPs) and secondary metabolism gene clusters involved in indole metabolism than the pathogens. In addition, these endophytes were able to improve Pi (phosphorus) accumulation and transport in rice by inducing the expression of Pi transport genes in rice. Specifically, inoculation with endophytes significantly increased Pi contents in roots at the early stage, while the Pi contents in inoculated shoots were significantly increased at the late stage. Our results not only provide important insights into endophyte-plant interactions but also provide strain and genome resources, paving the way for the agricultural application of Aspergillus endophytes.

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