Your browser doesn't support javascript.
Mostrar: 20 | 50 | 100
Resultados 1 - 20 de 71
Filtrar
Mais filtros










Base de dados
Intervalo de ano de publicação
1.
Oncol Rep ; 43(2): 727-735, 2020 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-31894290

RESUMO

The present study aimed to explore the relationship between the efficacy of epidermal growth factor receptor tyrosine kinase inhibitors (EGFR­TKIs) and the expression of the cancer stem cell (CSC)­related markers. Specimens of 72 cases of lung adenocarcinoma with significantly different therapeutic effects of the first­generation EGFR­TKI treatment were collected. The patients were divided into a sensitive group [progression­free survival (PFS) longer than 26 months] and a resistant group (PFS less than 5 months) according to the efficacy of first­line EGFR­TKI treatment. The expression of CSC­related markers (OCT4, SOX2, NANOG) in tumor tissues of the two groups was detected by immunohistochemical (IHC) staining, immunofluorescence and reverse transcriptase­quantitative polymerase chain reaction (RT­qPCR). IHC staining was quantified using H­scores. The unpaired nonparametric t­test was used to detect the differences in the results of IHC and RT­qPCR analyses between the groups. The Chi­square test was used to detect the differences in the clinical characteristics between the two groups. The t­test revealed that the IHC H­scores of SOX2 (P=0.003), OCT4 (P=0.036) and NANOG (P=0.032) were significantly higher in the resistant group than in the sensitive group. The results of RT­qPCR revealed that the relative levels of SOX2 (P=0.018), OCT4 (P=0.035) and NANOG (P=0.044) were significantly higher in the resistant group than in the sensitive group. The number of male patients, patients who smoked, patients with stage IV lung adenocarcinoma disease, and patients with poor differentiation was higher in the resistant group than in the sensitive group, with statistically significant differences. The poor efficacy of first­generation EGFR­TKIs for lung adenocarcinoma appears to be related to the increased expression of CSC markers.

2.
Lung Cancer ; 141: 72-77, 2020 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-31955003

RESUMO

OBJECTIVE: The role of micropapillary pattern (MIP) in EGFR-mutated NSCLC patients with brain metastases (BM) after complete surgical resection still remains unclear. Therefore, a retrospective study was conducted to evaluate the role of MIP in those patients. METHODS: This study included 332 stage I-III patients with EGFR-mutant lung adenocarcinoma and complete resection. Patients were classified in four groups: the MIP-positive patients without BM development, the MIP-negative patients without BM development, the MIP-positive patients with BM development and the MIP-negative patients with BM development. Intracranial disease-free survival (iDFS), systemic disease-free survival (DFS) and overall survival (OS) were evaluated. RESULTS: The median OS in the whole group was 70 months. The patients with MIP show inferior DFS (13 months vs. 22 months; P < 0.001) and OS (56 months vs. 74 months; P < 0.001). Furthermore, BM development was more likely to be found in patients with MIP (P = 0.001). In addition, the MIP-positive patients showed a significantly shorter iDFS compared with MIP-negative patients (14.5 months vs. 26 months; P < 0.001). Furthermore, the MIP-positive patients had significantly inferior iDFS in both BM as first line development groups (13 months vs. 19 months; P < 0.001) and BM as non-first line development groups (18 months vs. 33 months; P = 0.007). CONCLUSIONS: MIP was related to the earlier recurrence and shortened survival time. In addition, MIP was an independent poor prognostic factor for the increase of BM rate and the shortened time of BM development after surgery.

3.
Clin Infect Dis ; 2019 Nov 18.
Artigo em Inglês | MEDLINE | ID: mdl-31734699

RESUMO

BACKGROUND: Evaluation of a licensed inactivated EV71 vaccine is needed in a phase IV study with a large population to identify its effectiveness and safety for further application. METHODS: An open-label and controlled trial involving a large population of 155,995 children aged 6-71 months is performed; 40,724 were enrolled in the vaccine group and received 2 doses of inactivated EV71 vaccine at an interval of 1 month, and the remaining children were used as the control group. The EV71-infected hand, foot and mouth disease (HFMD) cases were monitored in the vaccine and control groups during a follow-up period of 14 months since the 28th days post inoculation through the local database of Notifiable Infectious Diseases Network. The effectiveness of the vaccine was estimated by comparing the incidence density in the vaccine group versus that in the control group based upon EV71 infected patients identified via laboratory testing. In parallel, the active and passive surveillance for safety of the vaccine was conducted by home or telephone visits and by using the Adverse Event Following Immunization (AEFI) system, respectively. RESULTS: An overall level of 89.7% (95% confidence interval [CI], 24.0 to 98.6) vaccine effectiveness (VE) against EV71 infection and a 4.58% rate of reported AEs were observed. Passive surveillance demonstrated a 0.31% rate of reported common minor reactions. CONCLUSIONS: The clinical protection and safety of the EV71 vaccine were demonstrated in the immunization of a large population. CLINICAL TRIALS REGISTRATION: NCT03001986.

4.
Rev Sci Instrum ; 90(8): 083704, 2019 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-31472646

RESUMO

Since the dark-field acoustic-resolution photoacoustic microscopy (AR-PAM) was invented over one decade ago, its powerful imaging capability made this system successful. In this work, we designed and tested an AR-PAM system whose key parts are relied on 3D printing and fiber bundles. This new design not only makes it much simpler to develop a robust PAM system, but also the illumination angle is adjustable to aid for different applications. Our simulation study and phantom experiments demonstrated that this design has the comparable performance with traditional dark-field AR-PAM.

5.
Lung Cancer ; 135: 138-144, 2019 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-31446986

RESUMO

OBJECTIVES: Whether epidermal growth factor receptor tyrosine-kinase inhibitors (EGFR-TKIs) plus local consolidative therapy (LCT) has survival benefit over EGFR-TKIs alone in lung adenocarcinoma patients with EGFR mutation and bone oligometastases remains controversial. MATERIALS AND METHODS: We conducted a retrospective study to assess the effects of LCT in lung adenocarcinoma patients with bone oligometastases and EGFR mutation. The primary endpoint was overall survival (OS); the secondary endpoints was progression-free survival (PFS). RESULTS: A total of 127 lung adenocarcinoma patients with EGFR mutation and bone oligometastases were assessed, including 65 patients received EGFR-TKIs alone (monotherapy group) and 62 patients received EGFR-TKIs plus local consolidative therapy (LCT) (combination group). Addition of LCT was associated with significantly longer OS (36.3 vs. 21.0 months, P = 0.01; hazard ratio [HR] = 0.537, 95% confidence interval [CI]: 0.360-0.801, p = 0.01) and PFS (14.0 vs. 8.1 months, P = 0.01; HR = 0.613, 95%CI: 0.427-0.879, p = 0.01) in the whole cohort. CONCLUSION: In lung adenocarcinoma patients with EGFR-mutation and bone oligometastases, LCT plus EGFR-TKIs therapy is associated with significantly longer OS and PFS compared with EGFR-TKIs therapy alone, indicating that LCT plus EGFR-TKIs therapy might be a better therapeutic option for this patient population.

6.
Target Oncol ; 14(4): 491, 2019 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-31317306

RESUMO

The article Epidermal Growth Factor Receptor (EGFR)-Tyrosine Kinase Inhibitors (TKIs) Combined with Chemotherapy Delay Brain Metastasis in Patients with EGFR-Mutant Lung Adenocarcinom, written by Changhui Li, Bo Zhang, Jindong Guo, Fang Hu, Wei Nie, Xiaoxuan Zheng, Lixin Wang, Yuqing Lou, Yinchen Shen, Baohui Han, Xueyan Zhang, was originally published electronically on the publisher's internet portal (currently SpringerLink) on 03 July 2019 with open access. With the author(s)' decision to step back from Open Choice, the copyright of the article changed on 17 July 2019 to © Springer Nature Switzerland AG 2019 and the article is forthwith distributed under the terms of copyright.

7.
Target Oncol ; 14(4): 423-431, 2019 08.
Artigo em Inglês | MEDLINE | ID: mdl-31270661

RESUMO

BACKGROUND: Whether epidermal growth factor receptor (EGFR) tyrosine kinase inhibitors (TKIs) combined with chemotherapy can delay the occurrence of brain metastasis (BM) is unclear. OBJECTIVE: This retrospective study aimed to evaluate whether EGFR-TKIs combined with chemotherapy can delay BM and decrease the incidence of BM as initial progression. PATIENTS AND METHODS: The data of 100 patients with EGFR-mutant advanced lung adenocarcinoma were retrospectively reviewed. The patients had no BM at initial diagnosis, and BM occurred during the treatment. Patients received EGFR-TKI only or EGFR-TKI combined with chemotherapy. Intracranial progression-free survival (iPFS), systemic progression-free survival (PFS), and overall survival (OS) were evaluated. RESULTS: The overall median OS was 39 months (95% confidence interval (CI), 35.6-42.4 months). The median OS of EGFR-TKI combined with chemotherapy and EGFR-TKI only are 41 months (95% CI 35.5-46.5 months) and 39 months (95% CI 36.8-41.2 months), respectively. Patients in the combination treatment group had longer PFS (16 vs. 10 months; P = 0.030) and iPFS (21 vs. 14 months; P = 0.026). Further, as initial progression, fewer patients developed BM in the combined treatment group compared with the EGFR-TKI-only group (30.6% vs. 52.9%, P = 0.002) with a hazard ratio of 0.64 (95% CI 0.43-0.96). After controlling for significant covariables in a multivariable model, the different treatment strategies were independently associated with improved iPFS. CONCLUSIONS: In this retrospective analysis, EGFR-TKIs combined with chemotherapy could improve PFS. Further, the combined treatment could delay BM occurrence and decrease the incidence of BM as initial progression.

8.
Cancer Manag Res ; 11: 3433-3443, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31114377

RESUMO

Purpose: This study aimed to evaluate the efficacy of upfront whole-brain radiotherapy (WBRT) in EGFR-mutant lung adenocarcinoma patients with multiple brain metastases (BM). Methods: In this study, 195 patients with EGFR mutations who had multiple BM at preliminary diagnosis were included and retrospectively reviewed. Patients were admitted to receive the following treatments in a multi-disciplinary setting: upfront WBRT followed by EGFR-TKI, concurrent EGFR-TKI and WBRT and upfront EGFR-TKI followed by WBRT. A disease-specific graded prognostic assessment (DS-GPA) was performed for all the patients. The treatment response and overall survival (OS) were assessed as well. Results: The median OS of these patients was 27 months. Objective response rate (ORR) was significantly better in upfront WBRT group than other two groups (P=0.004). Moreover, patients who received upfront WBRT (n=67) had longer OS than the concomitant group (36 vs 25 months; P=0.006) and the upfront EGFR-TKI group (36 vs 25 months; P<0.0001). The prognosis of patients with different DS-GPA scores significantly differed (P<0.0001). In concomitant group and upfront EGFR-TKIs group, patients with higher DS-GPA scores of 2-3 had more favorable prognosis compared with those with lower DS-GPA scores of 0-1.5 (27 vs 25 months; P=0.023). Patients who received EGFR-TKIs concurrently with WBRT had longer OS than those received upfront EGFR-TKIs with high DS-GPA scores. (37 vs 17 months; P=0.023). Conclusion: The use of upfront WBRT for EGFR-mutated lung adenocarcinoma patients with multiple BM can improve ORR and OS. More importantly, patients with high DS-GPA scores are recommended to receive WBRT immediately after EGFR-TKIs therapy.

9.
Chin J Integr Med ; 2019 Mar 27.
Artigo em Inglês | MEDLINE | ID: mdl-30919241

RESUMO

OBJECTIVE: To systematically evaluate the protective effects of Humulus lupulus L. extract (HLE) on osteoporosis mice. METHODS: In vivo experiment, a total of 35 12-week-old female ICR mice were equally divided into 5 groups: the sham control group (sham); the ovariectomy with vehicle group (OVX); the OVX with estradiol valerate [EV, 0.2 mg/(kg•d)] the OVX with low- or high-dose HLE groups [HLE, 1 g/(kg•d) and 3 g/(kg•d)], 7 in each group. Treatment began 1 week after the ovariectomized surgery and lasted for 12 weeks. Bone mass and trabecular bone mircoarchitecture were evaluated by micro computed tomography, and bone turnover markers in serum were evaluated using enzyme-linked immunosorbent assay (ELISA) kits. In vitro experiment, osteoblasts and osteoclasts were treated with HLE at doses of 0, 4, 20 and 100 µg/mL. Biomarkers for bone formation in osteoblasts and bone resorption in osteoclasts were analyzed. RESULTS: Compared with the OVX group, HLE exerted bone protective effects by the increase of estradiol (P<0.05), the improvement of cancellous bone structure, bone mineral density (P<0.01) and the reduction of serum alkaline phosphatase (ALP), tartrate resistant acid phosphatase (TRAP), bone gla-protein, c-terminal telopeptides of type I collagen (CTX-I) and deoxypyridinoline levels (P<0.01 for all). In vitro experiment, compared with the control group, HLE at 20 µg/mL promoted the cell proliferation (P<0.01), and increased the expression of bone morphogenetic protein-2 and osteopontin levels in osteoblasts (both P<0.05). HLE at 100 µg/mL increased the osteoblastic ALP activities, and HLE at all dose enhanced the extracellular matrix mineralization (both P<0.01). Furthermore, compared with the control group, HLE at 20 µg/mL and 100 µg/mL inhibited osteoclastic TRAP activity (P<0.01), and reduced the expression of matrix metalloproteinase-9 and cathepsin K (both P<0.05). CONCLUSION: HLE may protect against bone loss, and have potentials in the treatment of osteoporosis.

10.
Appl Opt ; 58(4): 850-862, 2019 Feb 01.
Artigo em Inglês | MEDLINE | ID: mdl-30874129

RESUMO

Hyperspectral imaging (HSI) is a promising tool for microscopic histopathology studies. Pushbroom microscopic hyperspectral imaging systems are widely used because of their low cost and easy implementation. However, the spatial resolution of pushbroom HSI systems is limited by the width of the optical entrance slit. A narrower slit leads to longer exposure time and slower imaging speed. In this paper, we explored several spatial resolution enhancement algorithms, originally designed for remote-sensing hyperspectral imaging, for pushbroom microscopic HSI systems. Our results demonstrate that those algorithms could effectively achieve a higher spatial resolution without sacrificing imaging speed.

11.
J Cancer ; 10(5): 1254-1262, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-30854135

RESUMO

Introduction: Lung cancer diagnosed solely with the presence of intrathoracic metastases is classified as M1a. However, intrathoracic metastases can be further divided into different patterns. The objective of our study was to analyze the differences in survival between the different metastatic patterns of intrathoracic metastases in lung adenocarcinoma patients who have epidermal growth factor receptor (EGFR) mutations. Materials and Methods: Patients who were diagnosed only with intrathoracic metastasis between March 2011 and October 2016 and had EGFR-mutations were selected for this study. Prognosis was determined based on metastatic patterns by univariate and multivariate analysis. Results: A total of 137 patients (60 patients who only had pleural metastasis [Group A], 44 patients who only had contralateral lung metastasis [Group B] and 33 patients who had both pleural and contralateral lung metastasis with or without pericardial effusion [Group C]) were selected for this in the study. The median OS (overall survival) time was 38.1 (95%confidence interval [CI]: 27.8-48.4), 35.7(95%CI: 23.4-48.0), and 29.7(95%CI: 22.8-36.6) months for Group A, Group B, and Group C, respectively (p=0.037). Multivariate analysis demonstrated that Group A and Group B had higher OS compared to Group C (hazard ratio [HR]=0.524, 95%CI: 0.307-0.894, p=0.018; HR=0.473, 95%CI: 0.241-0.931, p=0.030, respectively) among lung adenocarcinoma patients with EGFR mutations. With regard to patients with pleural or contralateral metastasis only, OS benefit (p=0.579) was not significant between the two groups. Subgroup analysis demonstrated that OS benefit in Group A was significant in patients with N0-1 disease and 21L858R mutations but not in EGFR exon 19 deletions, N2-3 stage or T3-4 stage patients. Conclusion: The prognosis of EGFR-mutant lung adenocarcinoma patients diagnosed only with intrathoracic metastasis was different, indicating that M1a staging should be refined.

12.
Cancer Biol Ther ; 20(4): 413-422, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-30489194

RESUMO

OBJECTIVE: To investigate the role and mechanism of action of nicotinamide nucleotide transhydrogenase antisense RNA 1 (NNT-AS1) in osteosarcoma (OS). METHODS: Bioinformatic analysis suggested miR-320a as potential target of NNT-AS1. Influence of NNT-AS1 overexpression or knockdown on OS cell proliferation, colony-formation, apoptosis, migration and invasion capacity was first investigated. Expression levels of NNT-AS1, miR-320a, beta-catenin, RUNX2, IGF-1R, c-Myc, Cyclin D1 and MMP13 were also evaluated by RT-qPCR and western blotting accordingly. Xenograft models using U2OS and OS-732 cells with different NNT-AS1 gene modifications were constructed for tumor formation assay as well as evaluation of miR-320a, beta-catenin and RUNX2 expression in primary lesion. NNT-AS1-overexpressing U2OS cells and NNT-AS1-knockdown OS-732 cells were subject to miR-320a mimic and inhibitor transfection, respectively, to investigate the miR-320a dependency of the osteosarcoma-promoting role of NNT-AS1. RESULTS: NNT-AS1 overexpression significantly increased proliferation, survival and mobility of U2OS cells in vitro as well as its tumor formation ability in vivo, while NNT-AS1 knockdown showed opposite effect on OS-732 cells. In both in vitro and in vivo model, NNT-AS1 expression level significantly correlated with that of beta-catenin, RUNX2, IGF-1R, c-Myc, Cyclin D1 and MMP13 as well as Akt phosphorylation level, and inversely correlated with miR-320a expression. Transfection of miR-320a mimic significantly inhibiter the promoting effect of NNT-AS1 on cell proliferation, survival and mobility of U2OS cells, while miR-320 inhibitor partially rescued that of OS-732 cells. CONCLUSION: NNT-As1 functions as a cancer-promoting lncRNA by downregulating miR-320a, thus increasing the protein expression level of beta-catenin, RUNX2 and IGF-1R as well as activation of Akt in osteosarcoma.

13.
J Cell Physiol ; 234(5): 6758-6768, 2019 May.
Artigo em Inglês | MEDLINE | ID: mdl-30382588

RESUMO

This study aimed to investigate the synergistic effect of octamer-binding transcription factor 4 and sex determining region Y-box 2 (OCT4&SOX2)-specific cytotoxic T lymphocytes (CTLs) and programmed cell death protein 1 (PD-1) inhibitor on killing lung cancer stem-like cells (LCSCs) and their efficacy in treating drug-resistant lung cancer (DRLC) mice. OCT4&SOX2-specific CTLs and PD-1 inhibitor with differed doses were applied to treat PC9 cells and PC9 LCSCs. Cell counting kit-8 (CCK8) assay and flow cytometry (FCM) assay with carboxyfluorescein diacetate/succinimidyl ester staining target cells before treatment and propidium iodide (PI) staining dead cells after treatment were conducted to detect the cytotoxic activity. DRLC mice were constructed by injection of PC9 LCSCs suspension and Matrigel into left lung of SD mice. DRLC mice were randomly divided into five groups: control group, cytomegalovirus (CMV) pp65 CTLs group, OCT4&SOX2 CTLs group, PD-1 inhibitor group, and OCT4&SOX2 CTLs + PD-1 inhibitor group. In vitro, both CCK8 assay and FCM assay disclosed that OCT4&SOX2-specific CTLs plus PD-1 inhibitor presented with elevated cytotoxic activity on PC9 cells and PC9 LCSCs. In vivo, tumor volume and tumor weight were decreased, while tumor necrosis and tumor apoptosis were increased in OCT4&SOX2 CTLs group than CMV pp65 CTLs group and control group, and in OCT4&SOX2 CTLs + PD-1 inhibitor group than OCT4&SOX2 CTLs group and PD-1 inhibitor group. In addition, CD8 expression was increased while OCT4&SOX2 expressions were decreased in OCT4&SOX2 CTLs + PD-1 inhibitor group than OCT4&SOX2 CTLs group and PD-1 inhibitor group. In conclusion, OCT4&SOX2-specific CTLs and PD-1 inhibitor presented with the synergistic effect on killing LCSCs in vitro and treating DRLC mice in vivo.

14.
Clin Lung Cancer ; 20(1): e81-e90, 2019 01.
Artigo em Inglês | MEDLINE | ID: mdl-30341018

RESUMO

BACKGROUND: For oligometastatic lung adenocarcinoma patients with sensitive epidermal growth factor receptor (EGFR) mutations, the role of local consolidative therapy (LCT) remains debatable. The purpose of this study was to investigate the efficacy of LCT in oligometastatic lung adenocarcinoma patients. PATIENTS AND METHODS: We conducted a retrospective study to assess the effects of LCT on progression-free survival (PFS) and overall survival (OS). Patients with advanced-stage oligometastatic lung adenocarcinoma harboring sensitive mutation of epidermal growth factor receptor (EGFR) who received EGFR-tyrosine kinase inhibitor (TKI) or EGFR-TKI plus LCT were admitted to Shanghai Chest Hospital from January 2010 to December 2016. The PFS and OS of the 2 groups were accordingly analyzed. RESULTS: A total of 231 patients (143 patients who received LCT plus EGFR-TKI [combination group] and 88 patients who only received EGFR-TKI only [monotherapy group]) were included in this study. Median PFS was significantly longer in the combination group (15 months; 95% confidence interval [CI], 13.611-16.389) than in the monotherapy group (10 months; 95% CI, 8.936-11.064; hazard ratio = 0.610; 95% CI, 0.461, 0.807; P = .000). The median OS in the combination group was 34 months (95% CI, 27.889, 40.111) versus 21 months (95% CI, 18.445, 23.555) in the monotherapy group (hazard ratio = 0.593; 95% CI, 0.430-0.817; P = .001). CONCLUSION: LCT combined with TKIs therapy was a feasible method that significantly improved PFS and OS among oligometastatic lung adenocarcinoma patients with EGFR mutations, and it thus might be considered as an important medical treatment during clinical management.


Assuntos
Adenocarcinoma de Pulmão/terapia , Antineoplásicos/uso terapêutico , Quimioterapia de Consolidação , Neoplasias Pulmonares/terapia , Inibidores de Proteínas Quinases/uso terapêutico , Adenocarcinoma de Pulmão/mortalidade , Adulto , Idoso , Idoso de 80 Anos ou mais , Éteres de Coroa/uso terapêutico , Receptores ErbB/antagonistas & inibidores , Receptores ErbB/genética , Cloridrato de Erlotinib/uso terapêutico , Feminino , Gefitinibe/uso terapêutico , Humanos , Neoplasias Pulmonares/mortalidade , Masculino , Pessoa de Meia-Idade , Mutação/genética , Metástase Neoplásica , Pneumonectomia , Quinazolinas/uso terapêutico , Radiocirurgia , Estudos Retrospectivos , Análise de Sobrevida
15.
Minerva Endocrinol ; 44(3): 259-263, 2019 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-29205013

RESUMO

BACKGROUND: This paper aimed to investigate the regulating role of adipokine expression level in body fat distribution of patients with type 2 diabetes mellitus (T2MD) as well as its correlation with metabolic syndrome (MS). METHODS: One hundred forty patients with T2MD admitted in the Endocrinology Department of the First Affiliated Hospital of Liaoning University of Traditional Chinese Medicine from January 2017 to July 2017 were selected; their body height and weight were measured to calculate Body Mass Index (BMI); patients with a BMI ≤23.9 kg/m2 were included into control group (N.=49), and those with a BMI ≥24 kg/m2 into observation group (N.=91). Based on whether the patients were complicated with MS, they were divided into non-metabolic syndrome (N-MS) group (N.=79) and MS group (N.=61); the levels of serum dipeptidyl peptidase-4 (DPP-4), adiponectin (ADPN) and leptin as well as the contents of body fat and lean tissue of the two groups of patients were measured. RESULTS: The serum DPP-4 level in observation group was remarkably elevated compared with that in control group (P<0.05), but there were no significant differences in the leptin and ADPN levels between the two groups (P>0.05). The serum DPP-4 and leptin levels were positively correlated with the total body fat mass (FAT) of the patients in observation group (r=0.461, P=0.004; r=0.433, P=0.007); DPP-4 level had a positive correlation with trunk fat mass (TRUNK F) (r=0.545, P=0.001) and limb fat mass (LIMB F) (r=0.412, P=0.005); the leptin level was positively correlated with LIMB F (r=0.513, P=0.001); the leptin and ADPN levels were negatively correlated with the content of lean tissue (r=-0.476, P=0.001; r=-0.344, P=0.021). Compared with those in N-MS group, the levels of serum DPP-4 and leptin were increased significantly, while the ADPN level was decreased notably (P<0.05) in MS group. CONCLUSIONS: The adipokines DPP-4 and leptin in the serum can influence the body fat distribution of patients with T2MD; there is an important association of DPP-4, leptin and ADPN levels with MS, which may be used as therapeutic targets for multiple metabolism disorders of T2MD.


Assuntos
Adipocinas/sangue , Distribuição da Gordura Corporal , Diabetes Mellitus Tipo 2/complicações , Síndrome Metabólica/sangue , Absorciometria de Fóton , Adiponectina/sangue , Adulto , Índice de Massa Corporal , Dipeptidil Peptidase 4/sangue , Feminino , Humanos , Leptina/sangue , Masculino , Síndrome Metabólica/etiologia , Pessoa de Meia-Idade
16.
Biomed Eng Online ; 17(1): 187, 2018 Dec 29.
Artigo em Inglês | MEDLINE | ID: mdl-30594200

RESUMO

BACKGROUND: Optical imaging is one of the most common, low-cost imaging tools used for investigating the tumor biological behavior in vivo. This study explores the feasibility and sensitivity of a near infrared fluorescent protein mKate2 for a long-term non-invasive tumor imaging in BALB/c nude mice, by using a low-power optical imaging system. METHODS: In this study, breast cancer cell line MDA-MB-435s expressing mKate2 and MDA-MB-231 expressing a dual reporter gene firefly luciferase (fLuc)-GFP were used as cell models. Tumor cells were implanted in different animal body compartments including subcutaneous, abdominal and deep tissue area and closely monitored in real-time. A simple and low-power optical imaging system was set up to image both fluorescence and bioluminescence in live animals. RESULTS: The presence of malignant tissue was further confirmed by histopathological assay. Considering its lower exposure time and no need of substrate injection, mKate2 is considered a superior choice for subcutaneous imaging compared with fLuc. On the contrary, fLuc has shown to be a better option when monitoring the tumor in a diffusive area such as abdominal cavity. Furthermore, both reporter genes have shown good stability and sensitivity for deep tissue imaging, i.e. tumor within the liver. In addition, fLuc has shown to be an excellent method for detecting tumor cells in the lung. CONCLUSIONS: The combination of mKate2 and fLuc offers a superior choice for long-term non-invasive real-time investigation of tumor biological behavior in vivo.


Assuntos
Proteínas Luminescentes/metabolismo , Imagem Óptica/métodos , Animais , Neoplasias da Mama/patologia , Linhagem Celular Tumoral , Transformação Celular Neoplásica , Feminino , Humanos , Camundongos , Camundongos Endogâmicos BALB C
17.
J Biomed Nanotechnol ; 14(7): 1277-1286, 2018 Jul 01.
Artigo em Inglês | MEDLINE | ID: mdl-29944101

RESUMO

A novel dopamine biosensor based on carbon fiber microelectrodes (CFMEs) modified with copper(I) sulfide functionalized nanocomposites of the reduced graphene oxide (Cu2S/RGO) has been explored for the sensitive detection of dopamine and in vivo monitoring the neurotransmitters released by Drosophila's brain. The as-prepared Cu2S/RGO decorated microelectrodes were characterized by scanning electron microscopy (SEM) and X-ray photoelectron spectroscopy (XPS), and cyclic voltammetry (CV). Our observations demonstrate that Cu2S/RGO-CFMEs exhibited excellent catalytic activity and high selectivity for dopamine with relatively low detection limit (24 nM), wide linear range (i.e., from 0.1-20 µM) and outstanding reproducibility. Furthermore, the as-prepared new dopamine biosensor with high sensitivity and good stability was readily used to detect the amount of dopamine in the brain of drosophila, indicating the potential and promising application in the in vivo measurement of neurotransmitters without other electrochemical interference such as histidine, ascorbic acid, uric acid, and others.


Assuntos
Técnicas Biossensoriais , Cobre , Dopamina , Técnicas Eletroquímicas , Eletrodos , Grafite , Microeletrodos , Reprodutibilidade dos Testes , Sulfetos
18.
Chem Biol Interact ; 292: 24-29, 2018 Aug 25.
Artigo em Inglês | MEDLINE | ID: mdl-29932878

RESUMO

Elevated cyclooxygenase-2 (COX-2) closely associates with tumor progression and distant metastasis in various human cancers. However, the role of COX-2 in epithelial ovarian cancer (EOC), and its mechanistic details, remain poorly understood. In the present study, we tested hypothesis that COX-2 induces loss of expression of E-cadherin, with resulting promotion of cancer cells' invasiveness in ovarian cancer. First, we observed an inverse relationship between COX-2 and E-cadherin expression as COX-2 was enhanced but E-cadherin was decreased in surgically-resected specimens of EOC. Depletion of COX-2, by celecoxib treatment, resulted in attenuated nuclear translocation of Snail, and, in turn, significantly increased E-cadherin in EOC cell line SKOV3, which was established to be due to the reduced binding of Snail onto E-cadherin promoter. Such COX-2 inhibition resulted in reduced invasion of EOC cells, similar to what was achieved through Snail silencing in SKOV as well as ES-2 EOC cells. These results suggest that COX-2-Snail signaling plays a critical role in regulation of E-cadherin and might provide insights into mechanisms for paracrine inflammation-mediated aggressiveness in EOC.


Assuntos
Caderinas/genética , Caderinas/metabolismo , Celecoxib/farmacologia , Ciclo-Oxigenase 2/metabolismo , Neoplasias Epiteliais e Glandulares/fisiopatologia , Neoplasias Ovarianas/fisiopatologia , Fatores de Transcrição da Família Snail/metabolismo , Western Blotting , Caderinas/antagonistas & inibidores , Carcinoma Epitelial do Ovário , Linhagem Celular Tumoral , Regulação para Baixo , Feminino , Humanos , Inflamação/tratamento farmacológico , Neoplasias Epiteliais e Glandulares/tratamento farmacológico , Neoplasias Ovarianas/tratamento farmacológico , Reação em Cadeia da Polimerase , Regiões Promotoras Genéticas , Transdução de Sinais/efeitos dos fármacos
19.
Nanoscale ; 10(18): 8606-8614, 2018 May 10.
Artigo em Inglês | MEDLINE | ID: mdl-29696248

RESUMO

Photoacoustic microscopy (PAM) enables the measurement of properties associated with optical absorption within tissues and complements sophisticated technologies employing optical microscopy. An inadequate frequency response as determined by a piezoelectric ultrasonic transducer results, however, in poor depth resolution and inaccurate measurements of the coefficients of optical absorption. We developed a PAM system configured as an attenuated total reflectance sensor with a ten-layer graphene film sandwiched between a prism and water (the coupling medium) for photoacoustic (PA) wave detection. Transients of the PA pressure cause perturbations in the refractive index of the water thereby changing the polarization-dependent absorption of the graphene film. The signal in PA detection involves recording the difference in the temporal-varying reflectance intensity between the two orthogonally polarized probe beams. The graphene-based sensor has an estimated noise-equivalent-pressure sensitivity of ∼550 Pa over an approximately linear pressure response from 11.0 kPa to 55.0 kPa. Moreover, it enables a much broader PA bandwidth detection of up to ∼150 MHz, primarily dominated by a highly localized evanescent field. From the strong optical absorption of inherent hemoglobin, in vivo label-free PAM imaging provided a three-dimensional viewing of the microvasculature of a mouse ear. These results suggest great potential for graphene-based PAM in biomedical investigations, such as microcirculation studies.

20.
Sci Rep ; 8(1): 508, 2018 01 11.
Artigo em Inglês | MEDLINE | ID: mdl-29323243

RESUMO

To improve the novel Bacillus thuringiensis insecticidal gene cry2Ah1 toxicity, two mutants cry2Ah1-vp (V354VP) and cry2Ah1-sp (V354SP) were performed. SWISS-MODEL analysis showed two mutants had a longer loop located between ß-4 and ß-5 of domain II, resulting in higher binding affinity with brush border membrane vesicles (BBMV) of Helicoverpa armigera comparing with Cry2Ah1. The cry2Ah1, cry2Ah1-vp, and cry2Ah1-sp were optimized codon usage according to plant codon bias, and named mcry2Ah1, mcry2Ah1-vp, and mcry2Ah1-sp. They were transformed into tobacco via Agrobacterium-mediated transformation and a total of 4, 8, and 24 transgenic tobacco plants were obtained, respectively. The molecular detection showed the exogenous gene was integrated into tobacco genome, and successfully expressed at the transcript and translation levels. Cry2Ah1 protein in transgenic tobacco plants varied from 4.41 to 40.28 µg g-1 fresh weight. Insect bioassays indicated that all transgenic tobacco plants were highly toxic to both susceptible and Cry1Ac-resistant cotton bollworm larvae, and the insect resistance efficiency to Cry1Ac-resistant cotton bollworm was highest in mcry2Ah1-sp transgenic tobacco plants. The results demonstrated that cry2Ah1 was a useful Bt insecticidal gene to susceptible and Cry1Ac-resistant cotton bollworm and had potential application for insect biocontrol and as a candidate for pyramid strategy in Bt crops.


Assuntos
Proteínas de Bactérias/genética , Criptocromos/genética , Resistência a Inseticidas/genética , Mariposas/crescimento & desenvolvimento , Plantas Geneticamente Modificadas/metabolismo , Animais , Bacillus thuringiensis/genética , Proteínas de Bactérias/metabolismo , Proteínas de Bactérias/farmacologia , Criptocromos/metabolismo , Endotoxinas/farmacologia , Vesículas Extracelulares/metabolismo , Proteínas Hemolisinas/farmacologia , Controle de Insetos , Larva/efeitos dos fármacos , Larva/crescimento & desenvolvimento , Mariposas/efeitos dos fármacos , Mutagênese , Ligação Proteica , Estrutura Terciária de Proteína , Proteínas Recombinantes/análise , Proteínas Recombinantes/biossíntese , Proteínas Recombinantes/farmacologia , Tabaco/metabolismo
SELEÇÃO DE REFERÊNCIAS
DETALHE DA PESQUISA