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1.
Zhongguo Shi Yan Xue Ye Xue Za Zhi ; 27(6): 1966-1972, 2019 Dec.
Artigo em Chinês | MEDLINE | ID: mdl-31839068

RESUMO

OBJECTIVE: To study the mechanism of naoxintong capsule (NXT) -inhibiting peripheral ischemic inflammation. METHODS: Mice were randomly double-blindly divided into 3 groups: sham-operation group, model group and NXT group. Both model and NXT groups underwent the hind limb ischemia (HLI) surgery followed by oral gavage with saline or NXT, respectively, one hour after operation. Three days after operation, the percentages of neutrophils and macrophages in the gastrocnemius muscle were examined by flow cytomety and immunohistochemical method. The changes in gene and protein expressions induced by NXT were examined by real-time PCR and protein chip, respectively. The changes of signaling pathways were analyzed. RESULTS: Compared with sham oparation and model groups, NXT could decrease the ratios of neutrophils and macrophages in gastrocnemius inflammation site (P<0.01), and downregulate the mRNA expression of gene EMR1 (P<0.01). NXT reduced the expression of TNF-α and IL-1ß at both mRNA (P<0.001) and protein levels (P<0.05). The proteomic analysis showed that the use of NXT resulted in the expression changes of 13 proteins. The expression of 6 cytokines was increased, and the secretion of 7 proteins was upregulated. Besides, most of identified 13 proteins were involved in the function regulation of other immune cells. CONCLUSION: NXT can significantly alleviate ischemia-induced peripheral inflammation by reducing the ratio of immune cells and altering the expression patterns of mRNA and protein. The expression changes provide theoretical guidance and the potential targets for the clinical use of NXT in the treatment of ischemia-induced peripheral inflammatory diseases.


Assuntos
Medicamentos de Ervas Chinesas , Inflamação/tratamento farmacológico , Proteômica , Animais , Isquemia , Camundongos , Fator de Necrose Tumoral alfa
2.
Int J Biol Macromol ; 61: 7-16, 2013 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-23817095

RESUMO

Astragalus polysaccharide (APS) is an important bioactive component of Astragalus membranaceus Bunge (Leguminosae) that has been used in traditional Chinese medicine for treating muscle wasting, a serious complication with complex mechanism manifested as myofibers atrophy and satellite cells apoptosis. In this study, the anti-atrophy and anti-apoptotic activity of Astragalus polysaccharide (APS) was characterized in C2C12 skeletal muscle myotubes and myoblasts. APS inhibited dexamethasone-induced atrophy by restoring phosphorylation of Akt, m-TOR, P70s6k, rpS6 and FoxO3A/FoxO1. The targets that protected C2C12 myoblasts from damage by H2O2 were promoting cells proliferation and inhibiting cells apoptosis. The protective mechanisms involved mitochondrial pathway and death receptor pathway. Moreover, Antioxidant effect of APS was also detected in this work. Our findings suggested that APS could be explored as a protective and perhaps as a therapeutic agent in the management of muscle wasting.


Assuntos
Astrágalo (Planta)/química , Atrofia Muscular/metabolismo , Polissacarídeos/farmacologia , Animais , Apoptose/efeitos dos fármacos , Proteínas Reguladoras de Apoptose/metabolismo , Morte Celular/efeitos dos fármacos , Linhagem Celular , Proliferação de Células/efeitos dos fármacos , Dexametasona/efeitos adversos , Ativação Enzimática/efeitos dos fármacos , Peróxido de Hidrogênio/efeitos adversos , Camundongos , Fibras Musculares Esqueléticas/efeitos dos fármacos , Fibras Musculares Esqueléticas/metabolismo , Fibras Musculares Esqueléticas/patologia , Atrofia Muscular/induzido quimicamente , Mioblastos Esqueléticos/efeitos dos fármacos , Mioblastos Esqueléticos/metabolismo , Mioblastos Esqueléticos/patologia , Proteína Tirosina Fosfatase não Receptora Tipo 1/metabolismo , Proteínas Proto-Oncogênicas c-akt/metabolismo , Espécies Reativas de Oxigênio/metabolismo , Transdução de Sinais/efeitos dos fármacos , Serina-Treonina Quinases TOR/metabolismo
3.
J Hazard Mater ; 161(2-3): 1052-7, 2009 Jan 30.
Artigo em Inglês | MEDLINE | ID: mdl-18538927

RESUMO

To establish cost-efficient operating conditions for potential application of Fenton oxidation process to treat wastewater containing an azo dye Orange G (OG), some important operating parameters such as pH value of solutions, dosages of H(2)O(2) and Fe(2+), temperature, presence/absence of chloride ion and concentration of the dye, which effect on the decolorization of OG in aqueous solution by Fenton oxidation have been investigated systematically. In addition, the decolorization kinetics of OG was also elucidated based on the experimental data. The results showed that a suitable decolorization condition was selected as initial pH 4.0, H(2)O(2) dosage 1.0 x 10(-2)M and molar ratio of [H(2)O(2)]/[Fe(2+)] 286:1. The decolorization of OG enhanced with the increasing of reaction temperature but decreased as a presence of chloride ion. On the given conditions, for 2.21 x 10(-5) to 1.11 x 10(-4)M of OG, the decolorization efficiencies within 60 min were more than 94.6%. The decolorization kinetics of OG by Fenton oxidation process followed the second-order reaction kinetics, and the apparent activation energy E, was detected to be 34.84 kJ mol(-1). The results can provide fundamental knowledge for the treatment of wastewater containing OG and/or other azo dyes by Fenton oxidation process.


Assuntos
Compostos Azo/química , Corantes/química , Peróxido de Hidrogênio/química , Ferro/química , Oxigênio/química , Cloretos/química , Concentração de Íons de Hidrogênio , Íons , Cinética , Modelos Químicos , Pressão , Espectrofotometria Ultravioleta , Temperatura Ambiente , Fatores de Tempo
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