Your browser doesn't support javascript.
loading
Mostrar: 20 | 50 | 100
Resultados 1 - 20 de 41
Filtrar
Mais filtros










Base de dados
Intervalo de ano de publicação
1.
Comput Biol Med ; 142: 105179, 2022 03.
Artigo em Inglês | MEDLINE | ID: mdl-35074736

RESUMO

To improve the diagnosis of Lupus Nephritis (LN), a multilevel LN image segmentation method is developed in this paper based on an improved slime mould algorithm. The search of the optimal threshold set is key to multilevel thresholding image segmentation (MLTIS). It is well known that swarm-based methods are more efficient than the traditional methods because of the high complexity in finding the optimal threshold, especially when performing image partitioning at high threshold levels. However, swarm-based methods tend to obtain the poor quality of the found segmentation thresholds and fall into local optima during the process of segmentation. Therefore, this paper proposes an ASMA-based MLTIS approach by combining an improved slime mould algorithm (ASMA),  where ASMA is mainly implemented by introducing the position update mechanism of the artificial bee colony (ABC) into the SMA. To prove the superiority of the ASMA-based MLTIS method, we first conducted a comparison experiment between ASMA and 11 peers using 30 test functions. The experimental results fully demonstrate that ASMA can obtain high-quality solutions and almost does not suffer from premature convergence. Moreover, using standard images and LN images, we compared the ASMA-based MLTIS method with other peers and evaluated the segmentation results using three evaluation indicators called PSNR, SSIM, and FSIM. The proposed ASMA can be an excellent swarm intelligence optimization method that can maintain a delicate balance during the segmentation process of LN images, and thus the ASMA-based MLTIS method has great potential to be used as an image segmentation method for LN images. The lastest updates for the SMA algorithm are available in https://aliasgharheidari.com/SMA.html.


Assuntos
Processamento de Imagem Assistida por Computador , Nefrite Lúpica , Algoritmos , Humanos , Processamento de Imagem Assistida por Computador/métodos , Nefrite Lúpica/diagnóstico por imagem
2.
Comput Biol Med ; 142: 105181, 2022 03.
Artigo em Inglês | MEDLINE | ID: mdl-35016099

RESUMO

The artificial bee colony algorithm (ABC) has been successfully applied to various optimization problems, but the algorithm still suffers from slow convergence and poor quality of optimal solutions in the optimization process. Therefore, in this paper, an improved ABC (CCABC) based on a horizontal search mechanism and a vertical search mechanism is proposed to improve the algorithm's performance. In addition, this paper also presents a multilevel thresholding image segmentation (MTIS) method based on CCABC to enhance the effectiveness of the multilevel thresholding image segmentation method. To verify the performance of the proposed CCABC algorithm and the performance of the improved image segmentation method. First, this paper demonstrates the performance of the CCABC algorithm itself by comparing CCABC with 15 algorithms of the same type using 30 benchmark functions. Then, this paper uses the improved multi-threshold segmentation method for the segmentation of COVID-19 X-ray images and compares it with other similar plans in detail. Finally, this paper confirms that the incorporation of CCABC in MTIS is very effective by analyzing appropriate evaluation criteria and affirms that the new MTIS method has a strong segmentation performance.


Assuntos
COVID-19 , Processamento de Imagem Assistida por Computador , Algoritmos , Humanos , SARS-CoV-2 , Raios X
3.
Prostaglandins Other Lipid Mediat ; 158: 106609, 2022 02.
Artigo em Inglês | MEDLINE | ID: mdl-34954219

RESUMO

The arachidonic acid (AA) metabolism pathways play a key role in immunological response and inflammation diseases, such as asthma, etc. AA in cell membranes can be metabolized by lipoxygenases (LOXs) to a screen of bioactive substances that include leukotrienes (LTs), lipoxins (LXs), and eicosatetraenoic acids (ETEs), which are considered closely related to the pathophysiology of respiratory allergic disease. Studies also verified that drugs regulating AA LOXs pathway have better rehabilitative intervention for asthma. This review aims to summarize the physiological and pathophysiological importance of AA LOXs metabolism pathways in asthma and to discuss its prospects of therapeutic strategies.


Assuntos
Asma , Lipoxinas , Araquidonato 5-Lipoxigenase , Araquidonato Lipoxigenases , Asma/tratamento farmacológico , Humanos , Leucotrienos , Lipoxigenases
4.
IEEE Access ; 9: 45486-45503, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34786313

RESUMO

This paper has proposed an effective intelligent prediction model that can well discriminate and specify the severity of Coronavirus Disease 2019 (COVID-19) infection in clinical diagnosis and provide a criterion for clinicians to weigh scientific and rational medical decision-making. With indicators as the age and gender of the patients and 26 blood routine indexes, a severity prediction framework for COVID-19 is proposed based on machine learning techniques. The framework consists mainly of a random forest and a support vector machine (SVM) model optimized by a slime mould algorithm (SMA). When the random forest was used to identify the key factors, SMA was employed to train an optimal SVM model. Based on the COVID-19 data, comparative experiments were conducted between RF-SMA-SVM and several well-known machine learning algorithms performed. The results indicate that the proposed RF-SMA-SVM not only achieves better classification performance and higher stability on four metrics, but also screens out the main factors that distinguish severe COVID-19 patients from non-severe ones. Therefore, there is a conclusion that the RF-SMA-SVM model can provide an effective auxiliary diagnosis scheme for the clinical diagnosis of COVID-19 infection.

5.
Vet Microbiol ; 262: 109238, 2021 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-34560407

RESUMO

H9N2 subtype avian influenza virus (AIV) is an ongoing threat causing substantial loss to the poultry industry and thus necessitating the development of safe and effective vaccines against AIV. Given that inactivated vaccines are less effective in activating the mucosal immune system, we aimed to generate a vaccine that can actively engage the mucosal immunity which is the front line of the immune system. We generated a group of flagellin-based hemagglutinin globular head (HA1) fusion proteins and characterized their immunogenicity and efficacy. We found that Salmonella typhimurium flagellin (fliC) lacking the hypervariable domain (called herein as HA1-ΔfliC) was recognized by TLR5 and induced a moderate innate immune response compared to N-terminus of fliC (HA1-fliC) and C-terminus of fliC (fliC-HA1). The HA1-ΔfliC protein had increased adherence to the nasal cavity and trachea than HA1-fliC and fliC-HA1 and significantly increased the HA-specific sIgA titers. Our in vivo results revealed that chickens treated with HA1-ΔfliC had a significantly reduced level of viral loads in the cloaca and throat compared with chickens treated with inactivated vaccine. Overall, these results revealed that HA1-ΔfliC can protect chickens against H9N2 AIV by eliciting the efficient mucosal immune responses.


Assuntos
Células Epiteliais , Vírus da Influenza A Subtipo H9N2 , Vacinas contra Influenza , Influenza Aviária , Proteínas Recombinantes de Fusão , Animais , Anticorpos Antivirais/imunologia , Galinhas , Células Epiteliais/imunologia , Células Epiteliais/virologia , Flagelina/genética , Imunidade , Vírus da Influenza A Subtipo H9N2/genética , Vírus da Influenza A Subtipo H9N2/imunologia , Vacinas contra Influenza/imunologia , Influenza Aviária/imunologia , Influenza Aviária/prevenção & controle , Influenza Aviária/virologia , Proteínas Recombinantes de Fusão/imunologia
6.
Comput Biol Med ; 136: 104609, 2021 09.
Artigo em Inglês | MEDLINE | ID: mdl-34293587

RESUMO

This paper focuses on the study of multilevel COVID-19 X-ray image segmentation based on swarm intelligence optimization to improve the diagnostic level of COVID-19. We present a new ant colony optimization with the Cauchy mutation and the greedy Levy mutation, termed CLACO, for continuous domains. Specifically, the Cauchy mutation is applied to the end phase of ant foraging in CLACO to enhance its searchability and to boost its convergence rate. The greedy Levy mutation is applied to the optimal ant individuals to confer an improved ability to jump out of the local optimum. Furthermore, this paper develops a novel CLACO-based multilevel image segmentation method, termed CLACO-MIS. Using 2D Kapur's entropy as the CLACO fitness function based on 2D histograms consisting of non-local mean filtered images and grayscale images, CLACO-MIS was successfully applied to the segmentation of COVID-19 X-ray images. A comparison of CLACO with some relevant variants and other excellent peers on 30 benchmark functions from IEEE CEC2014 demonstrates the superior performance of CLACO in terms of search capability, and convergence speed as well as ability to jump out of the local optimum. Moreover, CLACO-MIS was shown to have a better segmentation effect and a stronger adaptability at different threshold levels than other methods in performing segmentation experiments of COVID-19 X-ray images. Therefore, CLACO-MIS has great potential to be used for improving the diagnostic level of COVID-19. This research will host a webservice for any question at https://aliasgharheidari.com.


Assuntos
COVID-19 , Processamento de Imagem Assistida por Computador , Algoritmos , COVID-19/diagnóstico por imagem , Humanos , Mutação , SARS-CoV-2 , Raios X
7.
Comput Biol Med ; 134: 104427, 2021 07.
Artigo em Inglês | MEDLINE | ID: mdl-34020128

RESUMO

Image segmentation is an essential pre-processing step and is an indispensable part of image analysis. This paper proposes Renyi's entropy multi-threshold image segmentation based on an improved Slime Mould Algorithm (DASMA). First, we introduce the diffusion mechanism (DM) into the original SMA to increase the population's diversity so that the variants can better avoid falling into local optima. The association strategy (AS) is then added to help the algorithm find the optimal solution faster. Finally, the proposed algorithm is applied to Renyi's entropy multilevel threshold image segmentation based on non-local means 2D histogram. The proposed method's effectiveness is demonstrated on the Berkeley segmentation dataset and benchmark (BSD) by comparing it with some well-known algorithms. The DASMA-based multilevel threshold segmentation technique is also successfully applied to the CT image segmentation of chronic obstructive pulmonary disease (COPD). The experimental results are evaluated by image quality metrics, which show the proposed algorithm's extraordinary performance. This means that it can help doctors analyze the lesion tissue qualitatively and quantitatively, improve its diagnostic accuracy and make the right treatment plan. The supplementary material and info about this article will be available at https://aliasgharheidari.com.


Assuntos
Algoritmos , Doença Pulmonar Obstrutiva Crônica , Difusão , Entropia , Humanos , Processamento de Imagem Assistida por Computador , Doença Pulmonar Obstrutiva Crônica/diagnóstico por imagem
8.
Reprod Domest Anim ; 56(5): 725-735, 2021 May.
Artigo em Inglês | MEDLINE | ID: mdl-33544931

RESUMO

Ziwuling black goats are typically found in loess plateaus regions and the Ziwuling Nature Reserve. Cryptorchidism is a common disease in this inbred goat, and its pathogenesis has been linked with the expression of insulin-like factor 3 (INSL-3). Therefore, this study aimed to investigate anatomical alterations caused by cryptorchism and the expression and distribution of INSL-3 in normal and cryptorchid testicular tissues. The testicular tissues of 6-month-old Ziwuling black goats were collected for microscopic analyses using histochemical, immunohistochemical, immunofluorescence and biometrical methods, as well as Western blotting to compare the expression and distribution of INSL-3. A lower expression of INSL-3 was observed in cryptorchid compared with normal testicular tissues (p < .01). Cryptorchidism caused a significant reduction in layers of spermatogenic epithelium and tubule areas in Ziwuling black goat (p < .01). The interstitial to seminiferous tubule area ratio was larger in cryptorchid than in normal group. Periodic Acid-Schiff (PAS) staining revealed pronounced positive bands in the interstitial tissue, while positive Alcian blue (AB) staining was not clear, and AB-PAS staining revealed a positive red band in the basement membrane of cryptorchid group. Immunofluorescence revealed a strong signal of INSL-3 expression in Sertoli and peritubular myoid cells, and moderate signal in Leydig and spermatogenic cells in the normal group. However, in cryptorchid testicular tissues, the signal of INSL-3 expression was strong in primary spermatocytes, occasional in Sertoli cells, limited in Leydig cells and absent in peritubular myoid cells. Furthermore, immunohistochemistry showed that INSL-3 expression was higher in normal testes compared with cryptorchid testicular tissues (p < .05), especially in primary spermatocytes and Sertoli cells. Collectively, our results indicate that cryptorchidism is closely related to the disorder of acid glycoprotein metabolism and the reduction in release of INSL-3 from Leydig cells. Moreover, Sertoli and peritubular myoid cells are crucial for INSL signalling and could underpin further research on the mechanism of cryptorchidism in animal.


Assuntos
Criptorquidismo/veterinária , Insulina/metabolismo , Testículo/metabolismo , Animais , Criptorquidismo/metabolismo , Criptorquidismo/patologia , Doenças das Cabras , Cabras , Células Intersticiais do Testículo , Masculino , Proteínas/metabolismo , Relaxina/metabolismo , Células de Sertoli/metabolismo
9.
Bioorg Chem ; 106: 104492, 2021 01.
Artigo em Inglês | MEDLINE | ID: mdl-33268008

RESUMO

Glucagon-like peptide-1 (GLP-1) receptor agonists as an effective approach for type 2 diabetes mellitus (T2DM) has been explored extensively, multi agonists based on GLP-1 may have better clinical benefits on obesity, Nonalcoholic steatohepatitis (NASH) and other metabolic diseases. To get multi agonists based on GLP-1, 15 conjugates were designed, synthesized, and tested for biological activity. GLP-1/glucagon dual receptor agonist E1 showed moderate long-acting hypoglycemic effect, CY-5 and CY-16 with GLP-1/GIP dual receptor agonistic activity exhibited longer duration of continuous blood glucose stabilization. The long-acting hypoglycemic effect was equal to that of semaglutide. Although they have lost the agonistic activity on glucagon receptor, chronic in vivo studies on T2DM mice and diet-induced obesity (DIO) mice showed that CY-5 can effectively reduce food intake, inhibit body weight gain, repair islets damage and improve the glucose tolerance. One month treatment on NASH mice showed that CY-5 can significantly lower the TG, TC, AST, ALT and LDL-C and increase the HDL-C. CY-5 can also improve the liver vacuolation, reduce fat accumulation and delay the process of the fibrosis. The liver protection effect is better than that of semaglutide. In summary, CY-5 is a promising candidate for the treatment of metabolic diseases and worthy for further development.


Assuntos
Diabetes Mellitus Tipo 2/tratamento farmacológico , Receptor do Peptídeo Semelhante ao Glucagon 1/agonistas , Hipoglicemiantes/farmacologia , Obesidade/tratamento farmacológico , Peptídeos/farmacologia , Receptores dos Hormônios Gastrointestinais/agonistas , Animais , Diabetes Mellitus Tipo 2/induzido quimicamente , Diabetes Mellitus Tipo 2/metabolismo , Relação Dose-Resposta a Droga , Descoberta de Drogas , Receptor do Peptídeo Semelhante ao Glucagon 1/metabolismo , Hipoglicemiantes/química , Masculino , Camundongos , Camundongos Endogâmicos C57BL , Camundongos Endogâmicos ICR , Estrutura Molecular , Obesidade/induzido quimicamente , Obesidade/metabolismo , Peptídeos/química , Ratos , Ratos Sprague-Dawley , Receptores dos Hormônios Gastrointestinais/metabolismo , Estreptozocina , Relação Estrutura-Atividade
10.
Exp Ther Med ; 20(3): 2903-2908, 2020 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-32765788

RESUMO

Efficacy and safety of vascular intervention combined with intravenous thrombolysis (IVT) was investigated in the treatment of acute intracranial arterial occlusion (AIAO). Ninety-two patients with AIAO treated in People's Hospital of Tongchuan from January 2014 to February 2016 were enrolled in this retrospective study. Forty-two patients were treated with vascular intervention (control group), while another 50 patients were treated with vascular intervention combined with IVT (study group). They were observed in terms of the improvement of clinical efficacy after treatment, the comparison of complications after treatment, the National Institute of Health Stroke Scale (NIHSS) score after treatment, the modified Rankin Scale (mRS) score at 3 months after treatment, and the Mini-Mental State Examination (MMSE) score at 3 months after treatment. Compared with those in the control group, patients in the study group had statistically significantly higher marked effectiveness and statistically significantly lower ineffectiveness (P=0.018), and a statistically significantly higher overall effective rate (P=0.042). The NIHSS score in the study group was statistically significantly lower than that in the control group after treatment (P=0.001). There was a statistically significant difference between the two groups in the mRS score at 3 months after treatment (Z=8.764, P>0.05). Compared with those in the control group, patients in the study group had a statistically significantly higher MMSE score after treatment, and a statistically significantly lower total incidence of postoperative complications (P=0.001). Vascular intervention combined with IVT has good efficacy and high safety in the treatment of AIAO, and the combination can statistically significantly improve patients' quality of life, so it has a good clinical application value.

11.
Crit Rev Microbiol ; 46(4): 420-432, 2020 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-32715811

RESUMO

The type I interferons (IFNs) represent the first line of host defense against influenza virus infection, and the precisely control of the type I IFNs responses is a central event of the immune defense against influenza viral infection. Influenza viruses are one of the leading causes of respiratory tract infections in human and are responsible for seasonal epidemics and occasional pandemics, leading to a serious threat to global human health due to their antigenic variation and interspecies transmission. Although the host cells have evolved sophisticated antiviral mechanisms based on sensing influenza viral products and triggering of signalling cascades resulting in secretion of the type I IFNs (IFN-α/ß), influenza viruses have developed many strategies to counteract this mechanism and circumvent the type I IFNs responses, for example, by inducing host shut-off, or by regulating the polyubiquitination of viral and host proteins. This review will summarise the current knowledge of how the host cells recognise influenza viruses to induce the type I IFNs responses and the strategies that influenza viruses exploited to evade the type I IFNs signalling pathways, which will be helpful for the development of antivirals and vaccines.


Assuntos
Evasão da Resposta Imune , Vírus da Influenza A/imunologia , Influenza Humana/imunologia , Interferon Tipo I/imunologia , Animais , Interações Hospedeiro-Patógeno , Humanos , Vírus da Influenza A/genética , Influenza Humana/genética , Influenza Humana/fisiopatologia , Influenza Humana/virologia , Interferon Tipo I/genética
12.
Bioorg Chem ; 95: 103538, 2020 01.
Artigo em Inglês | MEDLINE | ID: mdl-31901754

RESUMO

Oxyntomodulin (OXM) is an endogenous gastrointestinal hormone, which activates both the Glucagon-like peptide-1 receptor (GLP-1R) and the glucagon receptor (GCGR). However, OXM has shortcomings including poor GLP-1R agonism to control glycemia, short half-life and others. Inspired from the sequence relationship between OXM and glucagon, in this study, we introduced different C-terminus residues of GLP-1, exenatide and OXM to glucagon to get a series of hybrid peptides with enhanced GLP-1R activation. The formed glucagon-exenatide hybrid peptide shows higher GLP-1R activation properties than OXM. Then the peptides based on the glucagon-exenatide hybrid peptide were coupled with fatty acid side chains to prolong their half-lives. As a result, the most potent compound 16a could stimulate insulin secretion and maintain blood glucose in normal level for ~42.6 h in diabetic mice. 16a exhibited reduced HbA1c level in diabetic mice, lowered body weight significantly in obesity mice on chronic treatment assay. 16a, combined efficient GCGR/GLP-1R activity, is potential as novel treatment for obesity and diabetes. This finding provides new insights into balancing GLP-1/GCGR potency of glucagon-exenatide hybrid peptide and is helpful for discovery of novel anti-diabetic and bodyweight-reducing drugs.


Assuntos
Diabetes Mellitus Experimental/tratamento farmacológico , Receptor do Peptídeo Semelhante ao Glucagon 1/efeitos dos fármacos , Glucagon/química , Hipoglicemiantes/farmacologia , Oxintomodulina/química , Peptídeos/farmacologia , Perda de Peso/efeitos dos fármacos , Sequência de Aminoácidos , Animais , Glicemia/metabolismo , Ingestão de Energia , Teste de Tolerância a Glucose , Hipoglicemiantes/uso terapêutico , Camundongos , Obesidade/tratamento farmacológico , Obesidade/etiologia , Peptídeos/química , Peptídeos/uso terapêutico , Homologia de Sequência de Aminoácidos , Estreptozocina , Relação Estrutura-Atividade
13.
Bioorg Med Chem ; 27(20): 115070, 2019 10 15.
Artigo em Inglês | MEDLINE | ID: mdl-31471103

RESUMO

Exenatide is known as the first marketed GLP-1 agonist for antidiabetic treatment, but it need twice injection a day because of its fast clearance. This work aims to prolong the half-life of exenatide by modified with novel lipid chain. Four optimized exenatide analogs named as Cys12-Exenatide (1-39)-NH2, Cys40-Exenatide (1-39)-NH2, Cys12-Tyr22-Gln24-Glu28-Arg35-Exenatide (1-39)-NH2 and Tyr22-Gln24-Glu28-Arg35-Cys40-Exenatide (1-39)-NH2 were selected and applied for conjugation. Then a series of evaluations including GLP-1R activation assay were conducted, conjugation C2 was selected for further investigation. Glucoregulatory and insulin secretion assay and hypoglycemic duration test were accessed and showed that C2 was capable of comparable insulinotropic activities and glucose-lowering abilities with those of liraglutide and exenatide. Cell protective effects in INS-1 cells confirmed that C2 had relatively protection effects. Meanwhile, once daily injection of C2 to STZ-induced diabetic mice achieved long-term beneficial effects on glucose tolerance, body weight and blood chemistry. Acute feeding studies were evaluated in DIO mice. These results suggested that C2 is a promising agent for further investigation of its potential to treat diabetes patients with obese.


Assuntos
Glicemia/efeitos dos fármacos , Peso Corporal/efeitos dos fármacos , Diabetes Mellitus Experimental/tratamento farmacológico , Exenatida/farmacologia , Hipoglicemiantes/farmacologia , Lipídeos/farmacologia , Animais , Glicemia/metabolismo , Diabetes Mellitus Experimental/induzido quimicamente , Diabetes Mellitus Experimental/metabolismo , Relação Dose-Resposta a Droga , Exenatida/síntese química , Exenatida/química , Receptor do Peptídeo Semelhante ao Glucagon 1/agonistas , Teste de Tolerância a Glucose , Hipoglicemiantes/síntese química , Hipoglicemiantes/química , Lipídeos/química , Masculino , Camundongos , Camundongos Endogâmicos , Camundongos Obesos , Estrutura Molecular , Ratos , Ratos Sprague-Dawley , Estreptozocina , Relação Estrutura-Atividade
14.
Food Funct ; 10(10): 6543-6555, 2019 Oct 16.
Artigo em Inglês | MEDLINE | ID: mdl-31545328

RESUMO

Mastitis, a major disease affecting dairy cows, is most commonly caused by Staphylococcus aureus (S. aureus). Selenium (Se) can activate pivotal proteins in immune responses and regulate the immune system, and microRNA-155 (miR-155) is a key transcriptional regulator for inflammation-related diseases. We constructed the model of mouse mastitis in vivo and primary mouse mammary epithelial cells (MMECs) in vitro, which were induced by S. aureus. Se content of the mammary was estimated using an atomic fluorescence spectrophotometer. Histopathological analysis was performed via hematoxylin and eosin (H&E) staining. The mmu-miR-155-5p mimic was transfected in MMECs, and viability was determined through the MTT assay. Transfected efficiency was evaluated by qPCR and fluorescence staining. Cytokines including TNF-α, IL-1ß, IL-10 and TLRs were detected with qPCR. In addition, western blotting was used to evaluate the expression of the NF-κB and MAPKs signaling pathways. The results demonstrated that a Se-supplemented diet improved the content of Se in mammary tissues. Histopathological studies indicated that the mammary glands were protected in the Se-supplemented group after S. aureus infection. Se-supplementation suppressed the production of MPO, mmu-miR-155, TNF-α, IL-1ß, and TLR2 and significantly inhibited the phosphorylation of NF-κB and MAPKs in vivo and in vitro. All the data indicated that mmu-miR-155 played a pro-inflammatory role in our study, and Se-supplementation could suppress the expression of mmu-miR-155 to inhibit inflammation in S. aureus-induced mastitis in mice.


Assuntos
Doenças dos Bovinos/tratamento farmacológico , Mastite/tratamento farmacológico , MicroRNAs/genética , Selênio/administração & dosagem , Infecções Estafilocócicas/veterinária , Animais , Bovinos , Doenças dos Bovinos/genética , Doenças dos Bovinos/imunologia , Doenças dos Bovinos/microbiologia , Citocinas/genética , Citocinas/imunologia , Feminino , Regulação da Expressão Gênica , Mastite/genética , Mastite/imunologia , Mastite/microbiologia , Camundongos , MicroRNAs/imunologia , NF-kappa B/genética , NF-kappa B/imunologia , Infecções Estafilocócicas/genética , Infecções Estafilocócicas/imunologia , Infecções Estafilocócicas/microbiologia , Staphylococcus aureus/fisiologia , Receptor 2 Toll-Like/genética , Receptor 2 Toll-Like/imunologia
15.
Postgrad Med J ; 95(1127): 476-481, 2019 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-31332025

RESUMO

BACKGROUND: Patients with chronic obstructive pulmonary disease (COPD) commonly have higher intrinsic positive end-expiratory pressure (PEEPi). A breathing exercise programme strategy employing an appropriate PEEP may improve their pulmonary functional capacity, exercise tolerance and health-related quality of life. Breathing with an expiratory resistive load, which is a method of modulating spontaneous breathing against PEEPi, has not been fully studied in patients with COPD. The objective of this study was to investigate the role of changing spontaneous breathing in home-based conditions and regulating spontaneous breathing with breathing exercises in patients with COPD. METHODS: This was a prospective randomised trial including 64 patients with a diagnosis of stage III or IV COPD. Patients were randomised into two groups: standard treatment and standard treatment combined with breathing exercise rehabilitation. The effects of the treatments on the COPD assessment test (CAT) score, 6-minute walk test (6MWT) results and pulmonary function were compared at 0, 6, 12 and 18 months within and between the two groups. RESULTS: All outcomes showed no significant differences between the two groups at the beginning of the study, while the 6MWT and CAT scores exhibited clinically and statistically significant improvements (p<0.001) by the end of the study. At month 18, the change in the predicted percentage of forced expiratory volume in 1 s (FEV1%pred) differed between the two groups (p<0.05). In addition, there were statistically significant differences in the 6MWT results, CAT scores and FEV1%pred values between the baseline and month 18 (p<0.0001) in the intervention group. CONCLUSIONS: Improvements in 6MWT results, pulmonary function and CAT scores are associated with a successful response to breathing against PEEPi in patients with COPD. TRIAL REGISTRATION: This trial was registered at research registry.com (identifier research registry 4816).


Assuntos
Exercícios Respiratórios , Doença Pulmonar Obstrutiva Crônica/fisiopatologia , Doença Pulmonar Obstrutiva Crônica/terapia , Idoso , Feminino , Serviços de Assistência Domiciliar , Humanos , Masculino , Pessoa de Meia-Idade , Respiração com Pressão Positiva , Estudos Prospectivos
16.
BMC Bioinformatics ; 20(Suppl 8): 290, 2019 Jun 10.
Artigo em Inglês | MEDLINE | ID: mdl-31182028

RESUMO

BACKGROUND: It is of great clinical significance to develop an accurate computer aided system to accurately diagnose the breast cancer. In this study, an enhanced machine learning framework is established to diagnose the breast cancer. The core of this framework is to adopt fruit fly optimization algorithm (FOA) enhanced by Levy flight (LF) strategy (LFOA) to optimize two key parameters of support vector machine (SVM) and build LFOA-based SVM (LFOA-SVM) for diagnosing the breast cancer. The high-level features abstracted from the volunteers are utilized to diagnose the breast cancer for the first time. RESULTS: In order to verify the effectiveness of the proposed method, 10-fold cross-validation method is used to make comparison among the proposed method, FOA-SVM (model based on original FOA), PSO-SVM (model based on original particle swarm optimization), GA-SVM (model based on genetic algorithm), random forest, back propagation neural network and SVM. The main novelty of LFOA-SVM lies in the combination of FOA with LF strategy that enhances the quality for FOA, thus improving the convergence rate of the FOA optimization process as well as the probability of escaping from local optimal solution. CONCLUSIONS: The experimental results demonstrate that the proposed LFOA-SVM method can beat other counterparts in terms of various performance metrics. It can very well distinguish malignant breast cancer from benign ones and assist the doctor with clinical diagnosis.


Assuntos
Neoplasias da Mama/diagnóstico , Drosophila melanogaster/fisiologia , Máquina de Vetores de Suporte , Animais , Feminino , Humanos , Redes Neurais de Computação , Reprodutibilidade dos Testes
17.
Bioorg Chem ; 88: 102945, 2019 07.
Artigo em Inglês | MEDLINE | ID: mdl-31054430

RESUMO

Lytic peptides have been demonstrated to exhibit obvious advantages in cancer therapy with binding ability toward tumor cells via electrostatic attractions, which are lack of active targeting and aggregation to tumor tissue. In the present study, five conjugated lytic peptides were redesigned and constructed to target gonadotropin releasing hormone receptors (GnRHr), meanwhile, the disulfide bridge was introduced to achieve redox sensitive delivery based on the experience from the preliminary work of lytic peptides P3 and P7. YX-1, was considered to be the most promising for in-depth study. YX-1 possessed high potency (IC50 = 3.16 ±â€¯0.3 µM), low hemolytic effect, and cell membrane permeability in human A2780 ovarian cancer cells. Moreover, YX-1 had prominent pro-apoptotic activity by activating the mitochondria-cytochrome c-caspase apoptotic pathway. The study yielded the conjugate YX-1 with superior properties for antineoplastic activity, which makes it a promising potential candidate for targeting cancer therapy.


Assuntos
Antineoplásicos/farmacologia , Peptídeos/farmacologia , Receptores LHRH/antagonistas & inibidores , Antineoplásicos/síntese química , Antineoplásicos/química , Proliferação de Células/efeitos dos fármacos , Sobrevivência Celular/efeitos dos fármacos , Células Cultivadas , Relação Dose-Resposta a Droga , Ensaios de Seleção de Medicamentos Antitumorais , Humanos , L-Lactato Desidrogenase/metabolismo , Estrutura Molecular , Oxirredução , Peptídeos/síntese química , Peptídeos/química , Receptores LHRH/metabolismo , Relação Estrutura-Atividade
18.
Eur J Pharmacol ; 852: 151-158, 2019 Jun 05.
Artigo em Inglês | MEDLINE | ID: mdl-30807747

RESUMO

Inflammation plays an important role in acute lung injury (ALI). Hesperetin (HES), a natural flavanone and an aglycone of hesperidin, has established potent anti-inflammatory activity. The aim of this study was to evaluate the potential protective effect of HES on lipopolysaccharide (LPS)-induced ALI in mice and to illuminate its possible directly target. Results indicated that HES pretreatment significantly attenuated LPS-induced pulmonary pathological injury, total protein concentration, markedly decreased the number of neutrophils and the levels of inflammatory cytokines, TNF-α and IL-6, in ALI model in vivo and in vitro. Meanwhile, pretreatment with HES dramatically reduced myeloperoxidase (MPO) activity in LPS-induced ALI mice. Additionally, using molecular docking and co-immunoprecipitation assay, HES showed a directly bind with myeloid differentiation 2 (MD2), in which HES could inhibit MAPK activation, regulate IκB degradation, block the interaction MD2 and its co-receptor Toll-like receptor 4 (TLR4). Taken together, HES showed a significantly protective effect against LPS-induced ALI, which might be associated with MD2 protein. These results attested HES worthy of further progress into an adjunctive potential drug for the treatment for ALI.


Assuntos
Lesão Pulmonar Aguda/induzido quimicamente , Lesão Pulmonar Aguda/tratamento farmacológico , Citoproteção/efeitos dos fármacos , Hesperidina/farmacologia , Lipopolissacarídeos/farmacologia , Antígeno 96 de Linfócito/metabolismo , Terapia de Alvo Molecular , Lesão Pulmonar Aguda/metabolismo , Lesão Pulmonar Aguda/patologia , Animais , Regulação da Expressão Gênica/efeitos dos fármacos , Hesperidina/metabolismo , Hesperidina/uso terapêutico , Humanos , Pulmão/efeitos dos fármacos , Pulmão/metabolismo , Pulmão/patologia , Antígeno 96 de Linfócito/química , Sistema de Sinalização das MAP Quinases/efeitos dos fármacos , Camundongos , Camundongos Endogâmicos C57BL , Simulação de Acoplamento Molecular , Conformação Proteica , Células RAW 264.7 , Receptor 4 Toll-Like/metabolismo
19.
Pulm Pharmacol Ther ; 54: 53-59, 2019 02.
Artigo em Inglês | MEDLINE | ID: mdl-30528955

RESUMO

The main goal of this study was to evaluate the effects of arbutin (AR) on lipopolysaccharide (LPS)-induced lung injury. A lung injury rat model was established by intravenous LPS administration. We found that levels of inflammatory cytokines, including tumor necrosis factor-alpha (TNF-α), interleukin-1 beta (IL-1ß), and interleukin-6 (IL-6) in both serum and lung tissue were significant increased after LPS challenge. In addition, pathological conditions were examined in rat lungs, and it was demonstrated that AR-pretreatment reduced LPS-induced malondialdehyde (MDA) levels and increased LPS-induced superoxide dismutase (SOD) activity. Moreover, the expression of sirtuin1 (SIRT1), nuclear erythroid factor 2-related factor 2 (Nrf-2), heme oxygenase-1 (HO-1) as well as the phosphorylation of NF-κBp65 and IκBα were increased with LPS-induced lung injury, and were significantly restored by AR treatment. Together, our results indicated that SIRT1 is a potential therapeutic target in LPS-induced lung injury, and that AR may be a novel therapeutic in patients with acute lung injury.


Assuntos
Lesão Pulmonar Aguda/tratamento farmacológico , Arbutina/farmacologia , Sirtuína 1/metabolismo , Lesão Pulmonar Aguda/fisiopatologia , Animais , Citocinas/metabolismo , Modelos Animais de Doenças , Heme Oxigenase-1/metabolismo , Lipopolissacarídeos/toxicidade , Malondialdeído/metabolismo , Fator 2 Relacionado a NF-E2/metabolismo , Ratos , Ratos Sprague-Dawley , Superóxido Dismutase/metabolismo , Fator de Transcrição RelA/metabolismo
20.
Comput Methods Programs Biomed ; 153: 211-225, 2018 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-29157454

RESUMO

BACKGROUND AND OBJECTIVE: In countries with high prevalence of tuberculosis (TB), clinicians often diagnose tuberculous pleural effusion (TPE) by using diagnostic tests, which have not only poor sensitivity, but poor availability as well. The aim of our study is to develop a new artificial intelligence based diagnostic model that is accurate, fast, non-invasive and cost effective to diagnose TPE. It is expected that a tool derived based on the model be installed on simple computer devices (such as smart phones and tablets) and be used by clinicians widely. METHODS: For this study, data of 140 patients whose clinical signs, routine blood test results, blood biochemistry markers, pleural fluid cell type and count, and pleural fluid biochemical tests' results were prospectively collected into a database. An Artificial intelligence based diagnostic model, which employs moth flame optimization based support vector machine with feature selection (FS-MFO-SVM), is constructed to predict the diagnosis of TPE. RESULTS: The optimal model results in an average of 95% accuracy (ACC), 0.9564 the area under the receiver operating characteristic curve (AUC), 93.35% sensitivity, and 97.57% specificity for FS-MFO-SVM. CONCLUSIONS: The proposed artificial intelligence based diagnostic model is found to be highly reliable for diagnosing TPE based on simple clinical signs, blood samples and pleural effusion samples. Therefore, the proposed model can be widely used in clinical practice and further evaluated for use as a substitute of invasive pleural biopsies.


Assuntos
Derrame Pleural/diagnóstico , Tuberculose/patologia , Adolescente , Adulto , Idoso , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Máquina de Vetores de Suporte , Adulto Jovem
SELEÇÃO DE REFERÊNCIAS
DETALHE DA PESQUISA
...