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1.
Hua Xi Kou Qiang Yi Xue Za Zhi ; 39(2): 136-142, 2021 Apr 01.
Artigo em Chinês | MEDLINE | ID: mdl-33834667

RESUMO

OBJECTIVES: This study aims to construct endogenous exosomes abundantly loaded with miR-1 and investigate the role of exosome-mediated microRNA-1 (miR-1) delivery on CAL-27 cell proliferation. METHODS: Exosomes secreted by miR-1-overexpressing HEK293 cells (miR1-EXO) were purified via ultracentrifugation and subjected to transmission electron microscopy, nanoparticle analysis, Western blot analysis, and quantitative polymerase chain reaction (qPCR). CAL-27 cells were cocultured with exosomes secreted by HEK293 cells (CON-EXO) and miR1-EXO and equivalent phosphate buffer saline. The intracellular transport of exosomes was measured by using immunofluorescence, the expression of miR-1 and its target gene MET were investigated via qPCR, CAL-27 cell proliferation was measured through MTT assay, and cell cycle state was determined by applying flow cytometry. RESULTS: Electron microscopy revealed that miR1-EXO and CON-EXO were spherical or cup-shaped with an average diameter of approximately 110 nm. The well-known exosome markers CD9, Tsg101, and Alix were enriched. The expression of miR-1 in miR1-EXO was higher than that in CON-EXO (285.80±14.33 vs 1.00±0.06, P<0.000 1). After coculture with CAL-27 cells, miR1-EXO was internalized and unloaded miR-1 into CAL-27 cells. After coculture with miR1-EXO, the expression of miR-1 in CAL-27 cells was upregulated, whereas that of MET, the target gene of miR-1, was suppressed and the proliferation of CAL-27 cells was inhibited significantly. Normal oral keratinocyte cell proliferation was negligibly affected after coculture with miR1-EXO. CONCLUSIONS: Exosomes secreted from miR1-EXO cells could load abundant miR-1. Exosomal miR-1 delivered into CAL-27 cells by using miR1-EXO suppressed the expression of MET mRNA and inhibited cell proliferation.


Assuntos
Exossomos , MicroRNAs , Ciclo Celular , Proliferação de Células , Células HEK293 , Humanos
2.
J Cell Mol Med ; 25(7): 3449-3459, 2021 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-33660415

RESUMO

Tumour necrosis factor-α (TNF-α), a crucial cytokine, has various homeostatic and pathogenic bioactivities. The aim of this study was to assess the neuroprotective effect of ketamine against TNF-α-induced motor dysfunction and neuronal necroptosis in male C57BL/6J mice in vivo and HT-22 cell lines in vitro. The behavioural testing results of the present study indicate that ketamine ameliorated TNF-α-induced neurological dysfunction. Moreover, immunohistochemical staining results showed that TNF-α-induced brain dysfunction was caused by necroptosis and microglial activation, which could be attenuated by ketamine pre-treatment inhibiting reactive oxygen species production and mixed lineage kinase domain-like phosphorylation in hippocampal neurons. Therefore, we concluded that ketamine may have neuroprotective effects as a potent inhibitor of necroptosis, which provides a new theoretical and experimental basis for the application of ketamine in TNF-α-induced necroptosis-associated diseases.

3.
J Agric Food Chem ; 69(14): 4191-4203, 2021 Apr 14.
Artigo em Inglês | MEDLINE | ID: mdl-33787238

RESUMO

High internal phase emulsions (HIPEs) with an internal phase fraction of 84 vol % were prepared using carboxymethyl cellulose (CMC) and palm kernel oil ethoxylates (SOE-N-60) as a dual emulsifier. Effects of the oil-phase volume fraction, CMC concentration, and SOE-N-60 concentration on oil-in-water HIPEs stability were systematically studied by a Mastersizer 2000 instrument, Lx POL polarizing microscope, rheometer, etc. The bioavailability of tea tree oil can be effectively protected using HIPEs as a delivery system. The experimental results showed that, with the increase of the concentrations of CMC and SOE-N-60, the droplet size of HIPEs gradually decreases and the HIPEs showed good static stability. In addition, it was observed by scanning electron microscopy that the polyHIPEs materials using HIPEs stabilized by different SOE-N-60 and CMC concentrations as templates had different structures. Moreover, the synergism between CMC and SOE-N-60 surfactants plays a significant role in the preparation and stability of HIPEs.

4.
Chemosphere ; 276: 130102, 2021 Feb 26.
Artigo em Inglês | MEDLINE | ID: mdl-33684857

RESUMO

Metabolomics represents a powerful tool for measuring environmental exposures and biological responses to unveil potential mechanisms. Few studies have investigated the effects of exposure to fine particulate matter (PM2.5) longitudinally on serum metabolomics in regions with high-level PM2.5. Therefore, we examined the changes of serum metabolomics corresponding to individual PM2.5 exposure levels in spring and autumn among 63 healthy college students in Baoding city, Hebei, China. The metabolic profiling was determined by ultra-performance liquid chromatography coupled to quadrupole time-of-flight mass spectrometry. The average level of individual PM2.5 in the spring was 1.82-fold higher than in the autumn (240 µg/m3 vs 132 µg/m3). Males were exposed to a higher level of PM2.5 than females in the spring. Metabolic profiling was clearly separated by orthogonal partial least square-discriminant analysis in males but not in females. In the analysis of the associations between the metabolome and PM2.5 of the two seasons, the changes of 14 serum metabolites were significantly associated with PM2.5 in males. The metabolites related to heme metabolism (bilirubin, biliverdin), energy metabolism and oxidative stress (2-Octenoylcarnitine, N-Heptanoylglycine, and acetylcysteine), phospholipid metabolism (lysophosphatidic acid, phospholipid acid, and lysophosphatidylethanolamine), and tryptophan metabolism (N-Acetylserotonin, indolepyruvate, and melatonin) were decreased in the range of 2.16%-6.80% for each 10 µg/m3 increase of PM2.5, while thyrotropin-releasing hormone, glutathione, and phosphatidylethanolamine related to energy metabolism and oxidative stress, and phospholipid metabolism were increased in the range of 2.95%-4.90% for each 10 µg/m3 increase of PM2.5. This longitudinal study suggests that higher PM2.5 exposure may induce perturbations in serum metabolic signaling related to oxidative stress and inflammation, and males may be more prone to these metabolic perturbations.

5.
J Colloid Interface Sci ; 593: 359-369, 2021 Mar 09.
Artigo em Inglês | MEDLINE | ID: mdl-33744544

RESUMO

Most of the sedimentary carbonates deposited in the marine environments are composed of calcium carbonate minerals with varying amounts of incorporated Mg2+. However, understanding how interactions of impurities with carbonate and their incorporation affect sediments behavior remains a challenge. Here, a new insight is obtained by monitoring solution composition, morphology, and electrokinetic potential of carbonate particles formed in a spontaneous unseeded batch precipitation experiment using electrochemical and scanning electron microscopy methods. The solid composition and growth rate are extracted from changes in the bulk composition and fitted to chemical affinity rate law, revealing that the precipitation pathway consists of second-order dissolution and first-order precipitation. The molecular dynamics simulations show that the lattice strain induced by randomly substituting Ca2+ by Mg2+ stabilizes spherical nanoparticles and reduces their surface area and volume. Combining kinetics and thermodynamics insight, we conclude that variation in the carbonate bulk and interfacial energies, along with the solution supersaturation, lead to the dissolution-precipitation transformation pathway from Mg-rich to Mg-poor carbonate phase that preserves spherulitic morphology. Our findings are relevant for long-standing questions of how impurities influence diagenesis of carbonate sediments and spherulitic carbonate particles' origin.

6.
Sci Total Environ ; 772: 145510, 2021 Jun 10.
Artigo em Inglês | MEDLINE | ID: mdl-33770854

RESUMO

Coal fire sponges (CFS) are common in coal-fire areas. Due to the enrichment of Hg in CFS, large amounts of Hg are released by CFS into the atmosphere via natural weathering or solar radiation. Therefore, CFS should be of concern in Hg pollution management and control globally. In addition, CFS changes the Hg cycle path by capturing Hg from coal fires that would have entered the atmosphere. In this study, the concentration, distribution, species, and enrichment mechanism of CFS Hg were investigated. The results showed that the Hg concentration in CFS ranged from 1008 to 35,310 ng/g, with an average of 8932 ng/g (CFS number, n = 153). The Hg concentration of CFS in different types of land was found to be significantly inhomogeneous. To determine the status of subterranean spontaneous combustion, the Hg concentration was added, which can improve the effect of coal-fire monitoring. Compared to the background area topsoil, CFS was enriched in Hg, acid, SO42-, and total fluoride. The Hg species in CFS was primarily HgSO4, followed by HgO. However, the primary Hg species in the surrounding topsoil were HgCl2 and HgO. By the simulation experiment, it was determined that hydrofluoric acid (HF) was beneficial to activate the stable species in the coal-fire areas. HgCl2, HgO, or Hg0 were ionized by acid liquor or HF, which can promote Hg migration and increase the adsorbed ratio; in the presence of SO42-, the primary Hg species was HgSO4. Ultimately, Hg was absorbed by clay minerals and organic matter. The high-efficiency activation of steady Hg species by the coal-fire HF should be studied further.

7.
Nano Lett ; 2021 Feb 17.
Artigo em Inglês | MEDLINE | ID: mdl-33596656

RESUMO

Efficient endosomal escape is the most essential but challenging issue for siRNA drug development. Herein, a series of quaternary ammonium-based amphiphilic triblock polymers harnessing an elaborately tailored pH-sensitive hydrophobic core were synthesized and screened. Upon incubating in an endosomal pH environment (pH 6.5-6.8), mPEG45-P(DPA50-co-DMAEMA56)-PT53 (PDDT, the optimized polymer) nanomicelles (PDDT-Ms) and PDDT-Ms/siRNA polyplexes rapidly disassembled, leading to promoted cytosolic release of internalized siRNA and enhanced silencing activity evident from comprehensive analysis of the colocalization and gene silencing using a lysosomotropic agent (chloroquine) and an endosomal trafficking inhibitor (bafilomycin A1). In addition, PDDT-Ms/siPLK1 dramatically repressed tumor growth in both HepG2-xenograft and highly malignant patient-derived xenograft models. PDDT-Ms-armed siPD-L1 efficiently blocked the interaction of PD-L1 and PD-1 and restored immunological surveillance in CT-26-xenograft murine model. PDDT-Ms/siRNA exhibited ideal safety profiles in these assays. This study provides guidelines for rational design and optimization of block polymers for efficient endosomal escape of internalized siRNA and cancer therapy.

8.
Waste Manag ; 124: 273-282, 2021 Apr 01.
Artigo em Inglês | MEDLINE | ID: mdl-33639412

RESUMO

Polyethylene terephthalate (PET) is a widely used plastic material that may cause significant environmental pollution. China is a major global producer and consumer of PET. Previous studies have focused on the effects of toxic elements from PET (e.g., antimony leached from PET products) on the environment. However, detailed information about PET, particularly about the PET production, trade, use, and recycling in China, is limited. This study developed a network model of PET flows in China, including the production, market trade, manufacturing and use, and waste management and recycling stages. Based on this network model, the characteristics of PET flows during three periods of development for the PET industry were analyzed. The results show that the fiber and bottle manufacturing industries are the industries with the largest PET in-use stocks. The PET flows showed different characteristics in the terms of waste import, recycling, and disposal (mechanical recycling, chemical recycling, incineration, landfill, and discarding) in the different periods of PET industrial development. Notably, the amount of discarded PET was significant, and the treatment of waste PET would probably be a challenge in the future. Policies for improving the PET cycling system were provided on the basis of the study results to promote the management and sustainable utilization of PET materials.


Assuntos
Polietilenotereftalatos , Gerenciamento de Resíduos , China , Plásticos , Reciclagem
9.
J Hazard Mater ; 411: 125102, 2021 Jun 05.
Artigo em Inglês | MEDLINE | ID: mdl-33461012

RESUMO

Prenatal vanadium exposure is reported to be associated with restricted fetal growth and adverse birth outcomes. However, trimester-specific vanadium exposure in relation to early-childhood growth still remains unclear. A total of 1873 Chinese mother-infant pairs from whom a complete series of maternal urinary samples were collected over three stages of pregnancy were included from 2014 to 2016. The urinary concentrations of vanadium were analyzed. Children's anthropometric parameters were measured at birth, 6, 12 and 24 months. In boys, each doubling increase in vanadium concentrations at middle pregnancy was inversely associated with weight-for-length [- 9.07% (-17.21%, -0.93%)] and BMI z-score [- 9.66% (-18.05%, -1.28%)] at 24 months. Moreover, vanadium exposure at late pregnancy was negatively associated with weight [- 9.85% (-16.42%, -3.28%)], weight-for-length [- 11.00% (-18.40%, -3.60%)], and BMI z-scores [- 11.05% (-18.67%, -3.42%)] at 24 months in boys. However, the negative associations were not observed in girls, and we found evidence for sex difference (FDR p for interaction=0.01, 0.01 and 0.03 for weight, weight-for-length and BMI z-scores, respectively). Prenatal vanadium exposure may have an adverse effect on early-childhood growth, and the middle and late pregnancy could be windows of vulnerability for the adverse effects of vanadium exposure on growth development.

10.
Acta Pharmacol Sin ; 2020 Dec 11.
Artigo em Inglês | MEDLINE | ID: mdl-33311599

RESUMO

Astroglioma is the most common primary tumor in the central nervous system without effective treatment strategies. Temozolomide (TMZ) is a chemotherapeutic drug to treat astroglioma but exhibits low potency and has side effects. Therefore, there is an urgent need to develop new compounds to treat astroglioma. Dalbergia sissoo Roxb was the source of Dalbergia odorifera in traditional Chinese medicine (TCM) and has been clinically used as an anti-tumor medicine. 4-Methoxydalbergione (4MOD) is purified from Dalbergia sissoo Roxb., and shows an inhibitory effect on osteosarcoma, but its effects on astroglioma have not been reported. Here, we evaluate its anti-astroglioma effects on both in vitro and in vivo models. In cultured astroglioma U87 cells, 4MOD inhibited cell proliferation and induced cell apoptosis in a time- and concentration-dependent manner. Compared with TMZ, 4MOD exhibited a tenfold greater potency of anti-astroglioma effects. 4MOD effectively stalled the cell cycle in G2 phase. Transcriptome sequencing (RNA-seq) showed that 4MOD upregulated 158 genes and downregulated 204 genes that are mainly enriched in cell membrane, cell division, cell cycle, p53, TNF, and MAPK signaling pathways, which may underlie its anti-tumor mechanisms. In a nude mouse xenograft model transplanted with U87 cells, 10 mg/kg 4MOD slowed down tumor growth rate, while at 30 mg/kg dose, it reduced tumor size. Collectively, this study demonstrates that 4MOD is a potent native compound that remarkably inhibits U87 astroglioma growth in both in vitro and in vivo models.

11.
IEEE Trans Biomed Eng ; PP2020 Dec 16.
Artigo em Inglês | MEDLINE | ID: mdl-33326373

RESUMO

OBJECTIVE: In current surface acoustic wave (SAW) elastography field, wavelength-depth inversion model is a straightforward and widely used inversion model for depth-resolved elasticity profile reconstruction. However, the elasticity directly evaluated from the wavelength-depth relationship is biased. Thus, a new inversion model, termed weighted average phase velocity (WAPV) inversion model, is proposed to provide depth-resolved Young's modulus estimate with better accuracy. METHODS: The forward model for SAW phase velocity dispersion curve generation was derived from the numerical simulations of SAWs in layered materials, and inversion was implemented by matching the measured phase velocity dispersion curve to the one generated from the forward model using the least squares fitting. Three two-layer agar phantoms with different top-layer thicknesses and one three-layer agar phantom were tested to validate the proposed inversion model. Then the model was demonstrated on human skin at various sites (palm, forearm and back of hand) in-vivo. RESULTS: In multi-layered agar phantoms, depth-resolved elasticity estimates provided by the model have a maximal total inversion error of 15.2% per sample after inversion error compensation. In in-vivo human skin, the quantified bulk Young's moduli (palm: 212 ± 78 kPa; forearm: 32 ± 11 kPa and back of hand: 29 ± 8 kPa) are comparable to the reference values in the literature. CONCLUSION: The WAPV inversion model can provide accurate depth-resolved Young's modulus estimates in layered biological soft tissues. SIGNIFICANCE: The proposed model can predict depth-resolved elasticity in layered biological soft tissues with a reasonable accuracy which traditional wavelength-depth inversion model cannot provide.

12.
Lasers Surg Med ; 2020 Nov 08.
Artigo em Inglês | MEDLINE | ID: mdl-33161582

RESUMO

It is possible to enhance topical drug delivery by pretreatment of the skin with ablative fractional lasers (AFLs). However, the parameters to use for a given AFL to achieve the desired depth of ablation or the desired therapeutic or cosmetic outcome are hard to predict. This leaves open the real possibility of overapplication or underapplication of laser energy to the skin. In this study, we developed a numerical model consisting of a Monte Carlo radiative transfer (MCRT) code coupled to a heat transfer and tissue damage algorithm. The simulation is designed to predict the depth effects of AFL on the skin, verified with in vitro experiments in porcine skin via optical coherence tomography (OCT) imaging. Ex vivo porcine skin is irradiated with increasing energies (50-400 mJ/pixel) from a CO2 AFL. The depth of microscopic treatment zones is measured and compared with our numerical model. The data from the OCT images and MCRT model complement each other well. Nonablative thermal effects on surrounding tissue are also discussed. This model, therefore, provides an initial step toward a predictive determination of the effects of AFL on the skin. Lasers Surg. Med. © 2020 The Authors. Lasers in Surgery and Medicine published by Wiley Periodicals LLC.

13.
BMC Plant Biol ; 20(1): 515, 2020 Nov 11.
Artigo em Inglês | MEDLINE | ID: mdl-33176702

RESUMO

BACKGROUND: Stalk fracture caused by strong wind can severely reduce yields in maize. Stalks with higher stiffness and flexibility will exhibit stronger lodging resistance. However, stalk flexibility is rarely studied in maize. Stalk fracture of the internode above the ear before tasseling will result in the lack of tassel and pollen, which is devastating for pollination in seed production. In this study, we focused on stalk lodging before tasseling in two maize inbred lines, JING724 and its improved line JING724A1 and their F2:3 population. RESULTS: JING724A1 showed a larger stalk fracture angle than JING724, indicating higher flexibility. In addition, compared to JING724, JING724A1 also had longer and thicker stalks, with a conical, frustum-shaped internode above the ear. Microscopy and X-ray microcomputed tomography of the internal stalk architecture revealed that JING724A1 had more vascular bundles and thicker sclerenchyma tissue. Furthermore, total soluble sugar content of JING724A1, especially the glucose component, was substantially higher than in JING724. Using an F2:3 population derived from a JING724 and JING724A1 cross, we performed bulk segregant analysis for stalk fracture angle and detected one QTL located on Chr3: 14.00-19.28 Mb. Through transcriptome data analysis and ∆ (SNP-index), we identified two candidate genes significantly associated with high stalk fracture angle, which encode a RING/U-box superfamily protein (Zm00001d039769) and a MADS-box transcription factor 54 (Zm00001d039913), respectively. Two KASP markers designed from these two candidate genes also showed significant correlations with stalk fracture angle. CONCLUSIONS: The internode shape and glucose content are possibly correlated with stalk flexibility in maize. Two genes in the detected QTL are potentially associated with stalk fracture angle. These novel phenotypes and associated loci will provide a theoretical foundation for understanding the genetic mechanisms of lodging, and facilitate the selection of maize varieties with improved flexibility and robust lodging resistance.

14.
BMC Plant Biol ; 20(1): 490, 2020 Oct 27.
Artigo em Inglês | MEDLINE | ID: mdl-33109077

RESUMO

BACKGROUND: Kernel row number (KRN) is an important trait for the domestication and improvement of maize. Exploring the genetic basis of KRN has great research significance and can provide valuable information for molecular assisted selection. RESULTS: In this study, one single-locus method (MLM) and six multilocus methods (mrMLM, FASTmrMLM, FASTmrEMMA, pLARmEB, pKWmEB and ISIS EM-BLASSO) of genome-wide association studies (GWASs) were used to identify significant quantitative trait nucleotides (QTNs) for KRN in an association panel including 639 maize inbred lines that were genotyped by the MaizeSNP50 BeadChip. In three phenotyping environments and with best linear unbiased prediction (BLUP) values, the seven GWAS methods revealed different numbers of KRN-associated QTNs, ranging from 11 to 177. Based on these results, seven important regions for KRN located on chromosomes 1, 2, 3, 5, 9, and 10 were identified by at least three methods and in at least two environments. Moreover, 49 genes from the seven regions were expressed in different maize tissues. Among the 49 genes, ARF29 (Zm00001d026540, encoding auxin response factor 29) and CKO4 (Zm00001d043293, encoding cytokinin oxidase protein) were significantly related to KRN, based on expression analysis and candidate gene association mapping. Whole-genome prediction (WGP) of KRN was also performed, and we found that the KRN-associated tagSNPs achieved a high prediction accuracy. The best strategy was to integrate all of the KRN-associated tagSNPs identified by all GWAS models. CONCLUSIONS: These results aid in our understanding of the genetic architecture of KRN and provide useful information for genomic selection for KRN in maize breeding.

15.
J Environ Manage ; 276: 111205, 2020 Dec 15.
Artigo em Inglês | MEDLINE | ID: mdl-32906071

RESUMO

Increasingly, modern hydrological technologies are dynamically altering river water flow and drastically affecting river hydrogeochemical cycle regimes globally. The present study focused on the reservoir discharges of artificial floodwaters that influence spatiotemporal variations in the physicochemical and stable isotope compositions in the lower Yellow River (LYR) of China. The surface water samples were collected at the nine sites along the LYR during the pre-, inter-and post-flood periods. Then, the collected samples were analysed with the following standard method. The δD and δ18O slopes of the waterline clearly indicated that the prolonged reservoir water and different water flows impacted the hydrological cycle in the LYR regions compared to GMWL (global meteoric water line) and LMWL (local meteoric water line). The thermal stratification processes of the water in the largest reservoir slightly enriched the heavy isotopes, and physicochemical alteration was neglected. Statistical analysis of two-way ANOVA revealed that the p-values (p < 0.01, p < 0.05) were very strong for most of the variables between the periods, and the linear regression exhibited weak values (R2 = 0.253, R2 = 0.150) at the surface water temperature variations and suggested no significant influence of isotope composition. Overall, the Xiaolangdi reservoir water prolonged time rates, and artificial floodwater flow had a very small effect on the isotope composition; in particular, a large high turbidity concentration in the discharged artificial floodwaters was the only considerable ecological risk condition in the LYR. This kind of proper monitoring work is immensely important and prevents reservoirs from causing hydrological cycle impacts in the LYR and the adjacent coastal ecosystems.


Assuntos
Inundações , Rios , China , Ecossistema , Monitoramento Ambiental , Isótopos
16.
Sci Rep ; 10(1): 15851, 2020 09 28.
Artigo em Inglês | MEDLINE | ID: mdl-32985558

RESUMO

Waxy maize has many excellent characteristics in terms of its nutritional and economic value. In recent decades, the waxy maize germplasm has increased dramatically as a result of different selection methods. We collected 200 waxy maize inbred accessions from different origins to study their genetic diversity and phylogenetic relationships, and to identify new waxy mutations. A simple sequence repeat (SSR) analysis revealed wide genetic diversity among the 200 waxy maize accessions. The maize accessions were clustered into three groups. We sequenced the waxy gene from the first to the 14th exon. Nucleotide variation analysis of 167 waxy maize and 14 flint maize lines revealed some nucleotide differences in the waxy gene among different waxy maize groups, and much narrower nucleotide diversity in waxy maize than in flint maize. In a phylogenetic analysis, waxy maize carrying the same mutation allele clustered together, and waxy maize carrying different mutation alleles distributed in different groups; waxy maize was intermixed with flint maize in each branch, and wx-D7 waxy maize separated significantly from waxy maize lines carrying wx-D10, wx-124 and wx-hAT mutant alleles. The wx-hAT was a new waxy mutation identified in this study. It consisted of a 2286-bp transposon inserted into the middle of exon three of the waxy gene. A PCR marker specific for the wx-hAT allele was developed. These results will be useful for the utilization and preservation of the waxy maize germplasm, and the PCR marker has potential uses in waxy maize breeding programs.

17.
Zhong Nan Da Xue Xue Bao Yi Xue Ban ; 45(5): 489-494, 2020 May 28.
Artigo em Inglês, Chinês | MEDLINE | ID: mdl-32879095

RESUMO

To propose the architectural layout for the big general hospital in the face of public health emergencies, we analyzed the conditions, methods, problems and countermeasures for the reconstruction of the isolation ward from the existing medical building layout of a general hospital. The affected areas met the requirements of isolation ward in the reconstruction, and realized the corresponding partition and separation of people. But the cost of occupying the medical room should be concerned. General hospital should be alerted to potential risks of public health emergencies. The characteristics of different construction types, defects, and the function of the hospital should be considered in the construction, rebuilding, and expansion of the hospital, which shouldnot only meet the needs of the development of the hospital daily usage but also consider dealing with emergent public health events. We can adopt the reasonable layout, including setting up a firewall-like device between the channel and the floor, an ordinary ward at ordinary times, and an independent space for emergency by pulling down the gate. This strategy can not only avoid the problem of low utilization rate of the space occupied by the corresponding area in the ward for diseases spread by air and droplets, maximizing the efficiency of the medical site, but also avoid the problem of emergency response to the temporary reconstruction.


Assuntos
Emergências , Arquitetura de Instituições de Saúde , Hospitais Gerais , Saúde Pública , Serviço Hospitalar de Emergência , Humanos
19.
Gene ; 762: 145044, 2020 Dec 15.
Artigo em Inglês | MEDLINE | ID: mdl-32777528

RESUMO

OBJECTIVE: Gastric cancer is the most common malignant tumor. Most patients suffering from gastric cancer die of metastasis. The role of Atrial natriuretic peptide (ANP) in inhibiting and eliminating kinds of cancer cells has been reported. Aberrant activation of Hedgehog (Hh) signaling pathway contributes to initiation and progression of various malignancies. We have previously reported that the inhibitor of Hh, cyclopamine, reduces the metastatic activity of MGC-803 via inhibiting the expression of matrix metalloproteinases (MMP)-9. It remains to be further demonstrated that ANP has the suppressive effects on invasion and metastasis in gastric cancer via Hh-mediated MMP-9 production. METHODS: Transwell, western blot, qRT-PCR were used after application of ANP on MGC-803 gastric cancer cells to determine the levels of cell migration and invasion, protein levels of MMP-9 and Hh, as well as mRNAs of MMP-9 and Hh, respectively. RESULTS: It was demonstrated that the migration and invasion were significantly lower, MMP-9 and Hh as well as their mRNAs were lower as well, in ANP-treated MGC-803 gastric cancer cells than those in control. CONCLUSIONS: The expression of MMP-9 induced by aberrant activation of Hh in MGC-803 was inhibited by ANP, which may contribute to the inhibition of cell migration and invasion. These results suggested the potential of ANP to be used in gastric cancer therapy as an inhibitor targetting Hh signaling pathway to inhibit the proliferation as well as invasion and metastasis of gastric cancer.


Assuntos
Fator Natriurético Atrial/farmacologia , Proteínas Hedgehog/metabolismo , Metaloproteinase 9 da Matriz/metabolismo , Neoplasias Gástricas/metabolismo , Linhagem Celular Tumoral , Movimento Celular/efeitos dos fármacos , Proteínas Hedgehog/genética , Humanos , Metaloproteinase 9 da Matriz/genética , Transdução de Sinais/efeitos dos fármacos
20.
Front Plant Sci ; 11: 1057, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-32733529

RESUMO

Southern corn rust (SCR) caused by Puccinia polysora Underw. is a major disease causing severe yield losses during maize production. Here, we identified and mapped the SCR resistance gene RppM from the near-isogenic line Kangxiujing2416 (Jing2416K), which harbors RppM in the genetic background of the susceptible inbred line Jing2416. In this study, the inheritance of SCR resistance was investigated in F2 and F3 populations derived from a cross between Jing2416K and Jing2416. The observed 3:1 segregation ratio of resistant to susceptible plants indicated that the SCR resistance is controlled by a single dominant gene. Using an F2 population, we performed bulked segregant analysis (BSA) sequencing and mapped RppM to a 3.69-Mb region on chromosome arm 10S. To further narrow down the region harboring RppM, we developed 13 insertion/deletion (InDel) markers based on the sequencing data. Finally, RppM was mapped to a region spanning 110-kb using susceptible individuals from a large F2 population. Two genes (Zm00001d023265 and Zm00001d023267) encoding putative CC-NBS-LRR (coiled-coiled, nucleotide-binding site, and leucine-rich repeat) proteins, a common characteristic of R genes, were located in this region (B73 RefGen_v4 reference genome). Sequencing and comparison of the two genes cloned from Jing2416K and Jing2416 revealed sequence variations in their coding regions. The relative expression levels of these two genes in Jing2416K were found to be significantly higher than those in Jing2416. Zm00001d023265 and Zm00001d023267 are thus potential RppM candidates.

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