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1.
J Agric Food Chem ; 70(18): 5701-5714, 2022 May 11.
Artigo em Inglês | MEDLINE | ID: mdl-35502792

RESUMO

Understanding the microbial and chemical diversities, as well as what affects these diversities, is important for modern manufacturing of traditional fermented foods. In this work, Chinese dark teas (CDTs) that are traditional microbial fermented beverages with relatively high sample diversity were collected. Microbial DNA amplicon sequencing and mass spectrometry-based untargeted metabolomics show that the CDT microbial ß diversity, as well as the nonvolatile chemical α and ß diversities, is determined by the primary impact factors of geography and manufacturing procedures, in particular, latitude and pile fermentation after blending. A large number of metabolites sharing between CDTs and fungi were discovered by Feature-based Molecular Networking (FBMN) on the Global Natural Products Social Molecular Networking (GNPS) web platform. These molecules, such as prenylated cyclic dipeptides and B-vitamins, are functionally important for nutrition, biofunctions, and flavor. Molecular networking has revealed patterns in metabolite profiles on a chemical family level in addition to individual structures.

2.
BMC Gastroenterol ; 22(1): 216, 2022 May 03.
Artigo em Inglês | MEDLINE | ID: mdl-35505286

RESUMO

BACKGROUND AND AIMS: Cholecystectomy is performed for most gallbladder polyps (GPs). However, cholecystectomy results concerning complications in some patients. For benign GPs, adoption of gallbladder-preserving surgery is worth to recommend. We describe our experiences performing gallbladder-preserving polypectomy for GPs by embryonic-natural orifice transumbilical endoscopic surgery (E-NOTES) with a gastric endoscopy. METHODS: This is a retrospective study of patients with GPs who underwent gallbladder-preserving polypectomy by E-NOTES with a gastric endoscopy from April 2018 to September 2019 in our hospital. The operative time, intraoperative hemorrhage, intraoperative and postoperative complications, gallbladder emptying function were obtained and analyzed. RESULTS: The procedure was performed successfully in all 12 patients with 5 cases of single polyp and 7 cases of multiple polyps. The range of GPs size was 2 mm to 15 mm. The mean operation time was (95.33 ± 23.08) minutes (55-135 min). There were no adverse events including heavy bleeding, mortality and conversion to open surgery during operation. All patients were discharged in 4-5 days after surgery without postoperative complications such as delayed bleeding, fever, peritonitis, intra-abdominal abscess and abdominal wall incisional hernia. All patients were followed up at 1, 3, 6, and 12 months postoperation who had almost no visible incision on the umbilical region, no recurrent GPs. The gallbladder emptying function decreased one month after surgery, and gradually improved 3, 6 and 12 months after surgery. CONCLUSION: E-NOTES gallbladder-preserving polypectomy is a safe and effective option for patients with GPs and is close to scar-free surgery which can be performed in routine clinical practice.


Assuntos
Colecistectomia Laparoscópica , Doenças da Vesícula Biliar , Pólipos , Colecistectomia Laparoscópica/métodos , Endoscopia Gastrointestinal , Doenças da Vesícula Biliar/cirurgia , Humanos , Pólipos/cirurgia , Complicações Pós-Operatórias/etiologia , Complicações Pós-Operatórias/cirurgia , Estudos Retrospectivos , Resultado do Tratamento , Umbigo/cirurgia
3.
Clin Transl Med ; 12(5): e825, 2022 May.
Artigo em Inglês | MEDLINE | ID: mdl-35522895

RESUMO

AIMS: MORC family CW-type zinc finger 2 (MORC2), a GHKL-type ATPase, is aberrantly upregulated in multiple types of human tumors with profound effects on cancer aggressiveness, therapeutic resistance, and clinical outcome, thus making it an attractive drug target for anticancer therapy. However, the antagonists of MORC2 have not yet been documented. METHODS AND RESULTS: We report that MORC2 is a relatively stable protein, and the N-terminal homodimerization but not ATP binding and hydrolysis is crucial for its stability through immunoblotting analysis and Quantitative real-time PCR. The N-terminal but not C-terminal inhibitors of heat shock protein 90 (HSP90) destabilize MORC2 in multiple cancer cell lines, and strikingly, this process is independent on HSP90. Mechanistical investigations revealed that HSP90 N-terminal inhibitors disrupt MORC2 homodimer formation without affecting its ATPase activities, and promote its lysosomal degradation through the chaperone-mediated autophagy pathway. Consequently, HSP90 inhibitor 17-AAG effectively blocks the growth and metastatic potential of MORC2-expressing breast cancer cells both in vitro and in vivo, and these noted effects are not due to HSP90 inhibition. CONCLUSION: We uncover a previously unknown role for HSP90 N-terminal inhibitors in promoting MORC2 degradation in a HSP90-indepentent manner and support the potential application of these inhibitors for treating MORC2-overexpressing tumors, even those with low or absent HSP90 expression. These results also provide new clue for further design of novel small-molecule inhibitors of MORC2 for anticancer therapeutic application.


Assuntos
Antineoplásicos , Neoplasias da Mama , Adenosina Trifosfatases/genética , Antineoplásicos/farmacologia , Autofagia/genética , Neoplasias da Mama/tratamento farmacológico , Neoplasias da Mama/genética , Neoplasias da Mama/metabolismo , Feminino , Proteínas de Choque Térmico HSP90/genética , Proteínas de Choque Térmico HSP90/metabolismo , Humanos , Proteínas Oncogênicas , Fatores de Transcrição
4.
Mitochondrial DNA B Resour ; 7(4): 705-706, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-35493717

RESUMO

Syzygium odoratum (Lour.) DC. 1828 is a deciduous shrub in the family Myrtaceae. This species grows in sparse forests, especially in mountains, valleys, and broad-leaved evergreen forests along streams from 100 to 400 m above sea level. The primary distribution is in southern China (e.g. Guangdong, Guangxi, Hainan, etc.) and other south Asian countries (e.g. Vietnam). Here, we report and characterize the complete plastome from a cultivar of S. odoratum (Lour.) DC. 1828. The complete plastome is 159,352 bp in length with a typical structure and gene content found in angiosperms, including two inverted repeat regions (IRs) of 26,472 bp, a large single-copy (LSC) region of 87,993 bp, and a small single-copy (SSC) region of 18,415 bp. The plastome contains 132 genes, consisting of 84 protein-coding genes, 37 tRNA genes, and eight rRNA genes. The overall G/C content in the plastome of S. odoratum is 36.9%. By inferring phylogenetic relationships based on the existing data of related taxa, we find that S. odoratum is most closely related to Syzygium acuminatissimum, (Blume) DC. 1828 given the current sampling. The complete plastome sequence of S. odoratum will provide a useful resource for conservation genetics of this species, as well as for phylogenetic studies involving Myrtaceae.

5.
Artigo em Inglês | MEDLINE | ID: mdl-35362261

RESUMO

Surface-enhanced Raman spectroscopy (SERS) is an emerging powerful vibrational technique offering unprecedented opportunities in biomedical science for the sensitive detection of biomarkers and the imaging and tracking of biological samples. Conventional SERS detection is based on the use of plasmonic substrates (e.g., Au and Ag nanostructures), which exhibit very high enhancement factors (EF = 1010 -1011 ) but suffers from serious limitations, including light-induced local heating effect due to ohmic loss and expensive price. These drawbacks may limit detection accuracy and large-scaled practical applications. In this review, we focus on alternative approaches based on plasmon-free SERS detection on low-cost nanostructures, such as carbons, oxides, chalcogenides, polymers, silicons, and so forth. The mechanism of non-plasmonic SERS detection has been attributed to interfacial charge transfer between the substrate and the adsorbed molecules, with no photothermal side-effects but usually less EF compared with plasmonic nanostructures. The strategies to improve Raman signal detection, through the tailoring of substrate composition, structure, and surface chemistry, is reviewed and discussed. The biomedical applications, for example, SERS cell characterization, biosensing, and bioimaging are also presented, highlighting the importance of substrate surface functionalization to achieve sensitive, accurate analysis, and excellent biocompatibility. This article is categorized under: Diagnostic Tools > Diagnostic Nanodevices Diagnostic Tools > Biosensing Diagnostic Tools > In Vivo Nanodiagnostics and Imaging.

6.
J Healthc Eng ; 2022: 3845008, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-35378944

RESUMO

As a fatal lung disease, pulmonary fibrosis can cause irreversible damage to the lung, affect normal lung function, and eventually lead to death. At present, the pathogenesis of this kind of disease is not completely clear, and there is no radical cure. The main purpose of the treatment of this disease is to slow down the deterioration of pulmonary fibrosis. For this kind of disease, if it can be found early, it can be treated as soon as possible and the life of patients will be prolonged. Clinically, the diagnosis of pulmonary fibrosis depends on the relevant imaging examination, lung biopsy, lung function examination, and so on. Imaging data such as X-rays is a common examination means in clinical medicine and also plays an important role in the prediction of pulmonary fibrosis. Through X-ray, radiologists can clearly see the relevant lung lesions so as to make the relevant diagnosis. Based on the common medical image data, this paper designs related models to complete the prediction of pulmonary fibrosis. The model designed in this paper is mainly divided into two parts: first, this paper uses a neural network to complete the segmentation of lung organs; second, the neural network of image classification is designed to complete the process from lung image to disease prediction. In the design of these two parts, this paper improves on the basis of previous research methods. Through the design of a neural network with higher performance, more optimized results are achieved on the key indicators which can be applied to the real scene of pulmonary fibrosis prediction.


Assuntos
Fibrose Pulmonar , Humanos , Redes Neurais de Computação , Fibrose Pulmonar/diagnóstico por imagem , Radiografia , Tórax , Raios X
7.
J Biomol NMR ; 2022 Apr 07.
Artigo em Inglês | MEDLINE | ID: mdl-35389128

RESUMO

Rapid progress in machine learning offers new opportunities for the automated analysis of multidimensional NMR spectra ranging from protein NMR to metabolomics applications. Most recently, it has been demonstrated how deep neural networks (DNN) designed for spectral peak picking are capable of deconvoluting highly crowded NMR spectra rivaling the facilities of human experts. Superior DNN-based peak picking is one of a series of critical steps during NMR spectral processing, analysis, and interpretation where machine learning is expected to have a major impact. In this perspective, we lay out some of the unique strengths as well as challenges of machine learning approaches in this new era of automated NMR spectral analysis. Such a discussion seems timely and should help define common goals for the NMR community, the sharing of software tools, standardization of protocols, and calibrate expectations. It will also help prepare for an NMR future where machine learning and artificial intelligence tools will be common place.

8.
Opt Lett ; 47(7): 1758-1761, 2022 Apr 01.
Artigo em Inglês | MEDLINE | ID: mdl-35363728

RESUMO

This Letter proposes an effective light-field 3D saliency object detection (SOD) method, which is inspired by the idea that the spatial and angular information inherent in a light-field implicitly contains the geometry and reflection characteristics of the observed scene. These characteristics can provide effective background clues and depth information for 3D saliency reconstruction, which can greatly improve the accuracy of object detection and recognition. We use convolutional neural networks (CNNs) to detect the saliency of each elemental image (EI) with different viewpoints in an elemental image array (EIA) and the salient EIA is reconstructed by using a micro-lens array, forming a 3D salient map in the reconstructed space. Experimental results show that our method can generate high-quality 3D saliency maps and can be observed simultaneously from different angles and positions.


Assuntos
Aprendizado Profundo , Lentes , Imageamento Tridimensional/métodos , Redes Neurais de Computação
9.
J Colloid Interface Sci ; 621: 464-469, 2022 Apr 20.
Artigo em Inglês | MEDLINE | ID: mdl-35483178

RESUMO

Carbon dots (CDs), as new type of carbon-based nanoparticles, are considered to be an aggregate with irreversible polymerization. Achieving the reversible tunability of CDs luminescence based on their reversible polymerization is a challenging subject. Herein, we, for the first time, design and construct the blue-emitting CDs with reversible polymerization by a room-temperature Schiff base reaction between tannic acid and ethylenediamine. The formation of CDs is proven to be due to the crosslinking polymerization of precursors caused by imine bond. As a dynamic covalent bond, imine bond endows CDs with controllable structural transformation properties, and the prepared CDs can be depolymerized and polymerized reversibly by pH-controlled imine bond cleavage and re-formation. These properties of reversible fluorescence photoswitching make the CDs have a good application prospect in reversible information encryption.

10.
Water Res ; 218: 118429, 2022 Apr 09.
Artigo em Inglês | MEDLINE | ID: mdl-35483206

RESUMO

Electrode materials occupy most of the construction cost of the microbial electrochemical system (MES), and the low mechanical strength and poor electrochemical performance of the commonly used traditional carbon-based materials restrict the promotion and application of this technology. In this study, polymer-based three-dimensional (3D) honeycomb-structure (HS) materials with good mechanical properties were used as supporting materials. Graphene (GR), carbon nanotube (CNT), and polypyrrole (PPy) was separately chosen as a surface conductivity coating layer for preparing MES anodes. The introduction of GR, CNT, and PPy on HS increased surface roughness, hydrophilicity, O and N content, electrochemically active surface area, and decreased charge transfer internal resistance, which promoted the adhesion of microorganisms on their surface and enhanced the extracellular electron transfer process at the electrode/microbe interface. The CNT-HS anode system got the better maximal power density (1700.7 ± 149.0 mW/m2) of the three modified anode systems and 3.60 times that of MES using CC (471.8 ± 27.2 mW/m2) as the anode. The accelerated reactions of the redox species in the outer cell membrane, the promoted electron shuttle secretion, and the enhanced abundance of the tricarboxylic acid cycle-related functional genes in biofilm led to better performance of the CNT-HS anode system. The CNT-HS anode system also exhibited long-term operational stability (>6 months) and a good chemical oxygen demand degradation effect. Furthermore, CNT-HS material exhibited its cost advantage, and its projected cost is estimated to be about $1.8/m2, much lower than the currently used MES anodes ($8.2-548.2/m2). Considering the good mechanical properties, simple preparation process, low manufacturing cost, long-term stability, excellent bio-electrochemical performance, and good pollutant removal ability, HS-based anode has promising potential for high-performance MES in applications.

11.
EClinicalMedicine ; 47: 101379, 2022 May.
Artigo em Inglês | MEDLINE | ID: mdl-35480079

RESUMO

Background: Polycystic ovary syndrome (PCOS) is closely linked to metabolic disorders. Recent reports have identified galactose as having strong associations with metabolic disorders, however, the correlation between galactose and PCOS remains largely unknown. Methods: The serum galactose levels of 104 patients with PCOS and 98 controls were measured, and their relationships with several metabolic parameters were analyzed. The study took place at the Center for Reproductive Medicine at Shengjing Hospital of China Medical University, Shenyang, China from July 13 to Oct 20, 2020. The relationships between serum galactose and PCOS as well as PCOS-related insulin resistance were investigated via logistic regression analyses, and the performance of serum galactose as a potential biomarker for PCOS was evaluated using receiver operating characteristic curve analysis. Findings: Higher serum galactose levels were observed in the patients with PCOS than in the controls (p = 0.001). There was still a correlation between circulating galactose levels and PCOS after adjusting for covariates (p = 0.002; odds ratio (OR), 1.133; 95% confidence interval (CI) 1.047-1.227). Serum galactose levels were shown to be most closely related to the fasting serum insulin level (r = 0.370, p = 0.001) and were higher in the insulin-resistant subgroup than in the non-insulin-resistant subgroup of patients with PCOS (p = 0.001). There was no difference in serum galactose levels between the insulin-resistant and non-insulin-resistant subgroups of women in the control group (p > 0.05). Furthermore, higher serum galactose levels were shown to be associated with insulin resistance in PCOS (p = 0.004; OR, 26.017; 95% CI, 2.907-232.810). The area under the curve for galactose-mediated prediction of PCOS was 80.0%, with a sensitivity of 71.0% and a specificity of 86.4%. Interpretation: Higher circulating galactose levels correlate with PCOS and PCOS-related insulin resistance; therefore, it may serve as a potential biomarker for patients with PCOS. These findings require further validation in a study with a larger sample size. Funding: National Natural Science Foundation of China (No. 82,071,607 and 32,100,691); LiaoNing Revitalization Talents Program (No. XLYC1907071); Fok Ying Tung Education Foundation (No. 151,039); Key Research and Development Program of Liaoning Province (NO. 2,018,225,062); Outstanding Scientific Fund of Shengjing Hospital (No. 202,003).

12.
Environ Res ; 212(Pt B): 113220, 2022 Apr 06.
Artigo em Inglês | MEDLINE | ID: mdl-35398083

RESUMO

Atrial fibrillation (AF) is the most common sustained heart rhythm disorder associated with high mortality and morbidity. Limited studies have been conducted to assess the relationship between short-term exposure to ambient air pollution and AF attacks. This study aimed to explore the association between short-term ambient nitrogen dioxide (NO2) exposure and outpatient visits for AF in Xi'an, China. Data on daily AF outpatient visits and air pollutants from 2013 to 2019 (2555 days) were obtained. A time-series approach using over-dispersed Poisson generalized additive model (GAM) was employed, and stratified analyses were performed to investigate the potential modifying effects by season, age, and gender. A total of 8307 outpatient visits for AF were recorded. Increased levels of NO2 were associated with increased AF outpatient visits, and the most significant effect estimates were observed at lag 03: A 10 µg/m3 increase of NO2 at lag 03 was related to an elevation of 5.59% (95% CI: 2.67%, 8.51%) in daily outpatient visits for AF. Stratified analyses showed that there were no gender and age difference in the effect of NO2, while more obvious association was observed in cool seasons (October to March) than in warm seasons (April to September). In summary, short-term ambient NO2 exposure can be positively associated with daily outpatient visits for AF, especially in cool seasons. This work provided novel data that the association between air pollutants and AF can vary by seasons, further supporting that the prevention of cardiovascular health effects should be strengthened in winter.

13.
J Colloid Interface Sci ; 622: 50-61, 2022 Apr 25.
Artigo em Inglês | MEDLINE | ID: mdl-35489101

RESUMO

Photocatalytic reduction of Cr(VI) in water environments attracts more attention; however, the mechanisms involved in this process have not been clearly elucidated yet. In this study, the photocatalytic reduction of Cr(VI) by polydopamine modified Bi2.15WO6 (PDA/BWO) under visible light was conducted. Kinetics results show that PDA apparently accelerates the reduction of Cr(VI). The quasi-first-order kinetic constant of Cr(VI) reduction by 5PDA/BWO is 70.0 times that of the original BWO, reaching 0.070 min-1. X-ray photoelectron spectroscopy, Fourier transform infrared spectroscopy, and Raman analyses confirm the formation of ligand-to-metal charge transfer (LMCT) complex [Bi(III)OC] between PDA and BWO. The formed Bi(III)OC complex enhances visible light response and narrows the bandgap of PDA/BWO. The photoelectrochemical and photoluminescent characterization further reveals that the formed Bi(III)OC complex inhibits the recombination of carriers, thus enhancing the photocatalytic reactivity of PDA/BWO. Electrons, are derived from three paths, including dye sensitization, LMCT and bandgap excitation, contribute to Cr(VI) reduction by PDA/BWO. This study provides new insights on the paths of Cr(VI) reduction by PDA/BWO under visible light.

14.
J Cardiovasc Med (Hagerstown) ; 23(5): 308-317, 2022 May 01.
Artigo em Inglês | MEDLINE | ID: mdl-35486682

RESUMO

AIM: Lipid abnormalities often occur in patients with diabetes mellitus and the coexistence of diabetes mellitus and dyslipidaemia will increase the risk of cardiovascular diseases. However, the specific effects of sitagliptin on lipid control remain elusive in diabetic patients. The aim of this meta-analysis is to investigate the effects of sitagliptin alone or with other antidiabetic agents on serum lipid control. METHODS: PubMed, Cochrane Library, Embase and the ClinicalTrials.gov website were systematically searched from 2006 (the first year that sitagliptin entered market) to 16 January 2021. Eligible studies were randomized clinical trials (RCTs) of sitagliptin including outcomes of serum total cholesterol (TC), triglycerides, high-density lipoprotein cholesterol (HDL-C) or low-density lipoprotein cholesterol (LDL-C). RESULTS: A total of 14 RCTs with 2654 patients were identified. Treatment with sitagliptin alone or in combination with other antidiabetic agents significantly reduced serum TC [mean difference (MD) = -5.52 95% confidence interval (95% CI), -7.88 to -3.15; P < 0.00001] and LDL-C (MD = -0.07; 95% CI, -0.14 to 0.00; P < 0.00001) in patients with type 2 diabetes. No statistical significances were found in serum triglycerides (MD = 1.53; 95% CI, -8.22 to 11.28; P = 0.76) or HDL-C (MD = 0.65; 95% CI, -1.59 to 0.29; P = 0.18). Subgroup analyses suggest that sitagliptin can significantly decrease serum LDL-C, TC and triglyceride levels compared with placebo alone, and no statistical significance was found in comparison with the serum HDLC levels. CONCLUSION: Sitagliptin alone or in combination with other antidiabetic agents significantly reduces serum TC and LDL-C in patients with type 2 diabetes mellitus, while no significant difference was observed in serum triglycerides or HDL-C.


Assuntos
Diabetes Mellitus Tipo 2 , Fosfato de Sitagliptina , HDL-Colesterol , LDL-Colesterol , Diabetes Mellitus Tipo 2/diagnóstico , Diabetes Mellitus Tipo 2/tratamento farmacológico , Humanos , Hipoglicemiantes/uso terapêutico , Fosfato de Sitagliptina/efeitos adversos , Triglicerídeos
15.
Environ Sci Technol ; 56(9): 5520-5529, 2022 May 03.
Artigo em Inglês | MEDLINE | ID: mdl-35417140

RESUMO

Intake from food is considered an important route of human exposure to polychlorinated naphthalenes. To our knowledge, several studies have quantified dietary exposure but only in European countries and measuring only a few of the 75 congeners. In addition, the influence of source diversity on human exposure has seldom been assessed. We analyzed 192 composite food samples composed of 17,280 subsamples from 24 provinces in China to measure the concentrations of polychlorinated naphthalenes and estimate their daily intake and potential health risks on a national scale. The estimated cancer risk was in the range of 6.8 × 10-8 to 4.6 × 10-7. We compared our findings for 75 congeners with reports in the literature that quantified only 12 congeners. We estimate that these 12 congeners contribute only approximately 4% to the total mass daily intake of polychlorinated naphthalenes and 70% to the total toxic equivalent quantity, indicating underestimation of dietary exposure. The contributions of combustion-associated congeners to the total concentrations of polychlorinated naphthalenes were in the range of 31-52%, suggesting that the ongoing unintentional release of these compounds from industrial thermal processes is an important factor in polychlorinated naphthalene contamination and human exposure in China.


Assuntos
Exposição Dietética , Bifenilos Policlorados , China , Dibenzofuranos Policlorados , Monitoramento Ambiental , Humanos , Indústrias , Naftalenos/toxicidade
16.
Cell Death Dis ; 13(4): 408, 2022 Apr 28.
Artigo em Inglês | MEDLINE | ID: mdl-35484101

RESUMO

Triple-negative breast cancer (TNBC) is a highly lethal disease due to aggressive clinical phenotype and the lack of validated therapeutic targets. Our recent quantitative proteomic analysis of 90 cases of TNBC tissues and 72 cases of matched adjacent normal tissues revealed that the expression levels of BPTF-associated protein of 18 KDa (BAP18), a component of the MLL1 and NURF chromatin complexes, were upregulated in TNBC tissues relative to normal tissues. However, the biological function and the underlying mechanism of BAP18 in TNBC progression remain unexplored. Here, we report that BAP18 promoted TNBC cell proliferation, migration, and invasion in vitro and xenograft tumor growth and lung colonization in vivo. Mechanistic investigations revealed that S100 calcium-binding protein A9 (S100A9), a member of the S100 protein family that is frequently upregulated in breast tumors and acts as an oncogenic driver in breast cancer progression, was a downstream target gene of BAP18. BAP18 was recruited to histone H3 trimethylation at lysine 4 (H3K4me3)-marked promoter of S100A9 and enhanced its promoter activities. Notably, knockdown of BAP18 by short hairpin RNA in TNBC cells suppressed xenograft tumor growth in mice, the noted effect was partially reverted by re-expression of S100A9 in BAP18-depleted cells. Taken together, these results suggest that BAP18 promotes TNBC progression through, at least in part, transcriptional activation of oncogene S100A9, and represents a potential therapeutic target for TNBC.


Assuntos
Proteínas de Ligação a DNA/metabolismo , Neoplasias de Mama Triplo Negativas , Animais , Calgranulina B/genética , Calgranulina B/metabolismo , Linhagem Celular Tumoral , Cromatina , Regulação Neoplásica da Expressão Gênica/genética , Humanos , Camundongos , Oncogenes/genética , Proteômica , Ativação Transcricional , Neoplasias de Mama Triplo Negativas/patologia
17.
Dalton Trans ; 51(17): 6908-6917, 2022 May 03.
Artigo em Inglês | MEDLINE | ID: mdl-35445230

RESUMO

Bi3+ has gained increasing attention due to its abundant reserves, adjustable luminous colour and high chemical stability, therefore, Bi3+-activated luminescent materials have already been extensively applied in various fields. Herein, a novel blue-emitting CaNaSb2O6F:Bi3+ (CNSOF:Bi3+) phosphor with a pyrochlore-type structure with the space group Fd3̄m (277) was successfully synthesized. It exhibits a broad absorption band in the n-UV region (290-390 nm) and an ideal blue emission band centered at 441 nm. Interestingly, the wide emission peak of CNSOF:Bi3+ shows strongly temperature-dependent fluorescence properties and good thermal degradation resistance in the cycle temperature range from 298 K to 473 K, and the relative sensitivity is calculated to reach the maximum value of 2.34% K-1 at 423 K. Besides, the phosphor is different from a traditional optical temperature sensing material which shows the emission peak of trivalent rare earth ions. The wide emission peak makes the instrument insensitive to the peak shift, which dramatically reduces the requirement of the instrument, and the emission peak does not shift with the temperature to enhance the measurement stability, thus saving the cost. These results indicate that the CNSOF:Bi3+ blue emitting phosphor has potential applications in temperature sensing.

18.
Ecotoxicol Environ Saf ; 236: 113475, 2022 May 01.
Artigo em Inglês | MEDLINE | ID: mdl-35364508

RESUMO

Most aquatic ecosystems are at risk of being polluted by new environmental pollutant nanoparticles. As the main food source of zooplankton, the biomass of algae always fluctuates. Cladocerans, an important part of zooplankton, are usually be simultaneously exposed to different abundance of algae and nanoparticles in aquatic environment. To evaluate the combined effects of food abundance and ZnO nanoparticles concentration on the development and early reproductive performance of cladocerans, we exposed Daphnia magna, a common and representative model organism in cladocerans, to the combinations of different abundances of Chlorella pyrenoidosa and different concentrations of ZnO nanoparticles, recorded the key life-history traits, and used multiple models to fit the data. Results showed that high level of ZnO nanoparticles and low abundance Chlorella had an interactively negative effect on the life history of D. magna. When D. magna was exposed to ZnO nanoparticles, some life history traits, such as survival time, body length at maturation, and offspring per female, increased exponentially with the increase of food abundance, and then reached a theoretical maximum value, whereas some other life history traits, such as time to maturation and time to first brood, showed an opposite trend. However, higher Chlorella abundance reduced the negative effect of ZnO nanoparticles on D. magna, but the negative effect could not be eliminated with the increase of food abundance. Below Chlorella 0.30 mg C L-1, food plays a decisive role, while at or above this threshold, ZnO nanoparticles play a decisive role. Therefore, the effect of different ZnO nanoparticles concentrations can be fully reflected only when food is sufficient, and the negative effects of food shortages may mask the toxic effects of ZnO nanoparticles on D. magna. The findings indicated that the effects of food abundance should be considered in evaluating the realistic impact of pollutants on zooplankton.


Assuntos
Chlorella , Nanopartículas , Poluentes Químicos da Água , Óxido de Zinco , Animais , Daphnia , Ecossistema , Feminino , Nanopartículas/toxicidade , Reprodução , Poluentes Químicos da Água/toxicidade , Óxido de Zinco/toxicidade , Zooplâncton
19.
Artigo em Inglês | MEDLINE | ID: mdl-35411490

RESUMO

PURPOSE: To analyze the prognostic value of total, bioavailable and free 25-hydroxyvitamin D [25(OH)D] as well as vitamin D-binding protein (VDBP) in patients with non-small cell lung cancer (NSCLC). METHODS: We prospectively collected and analyzed data for 395 patients diagnosed with NSCLC between January 2016 and December 2018 in two university-affiliated hospitals. Total and free 25(OH)D and VDBP were measured directly, and bioavailable 25(OH)D was calculated using a validated formula. Their prognostic values were evaluated by Cox proportional hazards model, and hazard ratios (HRs) and 95% confidence intervals (CIs) were calculated. RESULTS: Patients with NSCLC had significantly lower levels of total, bioavailable, and free 25(OH)D and higher VDBP levels in comparison to healthy controls (all p < 0.001). In multivariate analyses, higher levels of total, bioavailable, and free 25(OH)D were independently associated better overall survival (OS) and progression-free survival (PFS). For OS, the adjusted HRs were 0.58 (95% CI, 0.40-0.87; p for trend = 0.008), 0.45 (95% CI, 0.30-0.67; p for trend < 0.001) and 0.49 (95% CI, 0.33-0.73; p for trend < 0.001) for the highest versus the lowest tertile of total, bioavailable and free 25(OH)D, respectively. The corresponding adjusted HRs for PFS were 0.61 (95% CI, 0.43-0.86; p for trend = 0.006), 0.56 (95% CI, 0.40-0.80; p for trend = 0.001) and 0.60 (95% CI, 0.42-0.85; p for trend = 0.004), respectively. However, VDBP was not associated with either OS or PFS. CONCLUSION: The current study suggested that total, bioavailable and free 25(OH)D may be reliable prognosis indicators in NSCLC patients, though the optimal 25(OH)D form for NSCLC prognosis remains to be assessed in future studies.

20.
Inorg Chem ; 61(16): 6138-6148, 2022 Apr 25.
Artigo em Inglês | MEDLINE | ID: mdl-35412316

RESUMO

Single-crystal structures of myo-inositol complexes with erbium ([Er2(C6H11O6)2(H2O)5Cl2]Cl2(H2O)4, denoted ErI hereafter) and strontium (Sr(C6H12O6)2(H2O)2Cl2, denoted SrI hereafter) are described. In ErI, deprotonation occurs on an OH of myo-inositol, although the complex is synthesized in an acidic solution, and the pKa values of all of the OHs in myo-inositol are larger than 12. The deprotonated OH is involved in a µ2-bridge. The polarization from two Er3+ ions activates the chemically relatively inert OH and promotes deprotonation. In the stable conformation of myo-inositol, there are five equatorial OHs and one axial OH. The deprotonation occurs on the only axial OH, suggesting that the deprotonation possesses characteristics of regioselectivity/chiral selectivity. Two Er3+ ions in the µ2-bridge are stabilized by five-membered rings formed by chelating Er3+ with an O-C-C-O moiety. As revealed by the X-ray crystallography study, the absolute values of the O-C-C-O torsion angles decrease from ∼60 to ∼45° upon chelating. Since the O-C-C-O moiety is within a six-membered ring, the variation of the torsion angle may exert distortion of the chair conformation. Quantum chemistry calculation results indicate that an axial OH flanked by two equatorial OHs (double ax-eq motif) is favorable for the formation of a µ2-bridge, accounting for the selectivity. The double ax-eq motif may be used in a rational design of high-performance catalysts where deprotonation with high regioselectivity/chiral selectivity is carried out.


Assuntos
Inositol , Catálise , Cristalografia por Raios X , Inositol/química , Modelos Moleculares , Conformação Molecular
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