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1.
J Neurosurg ; : 1-12, 2020 Mar 13.
Artigo em Inglês | MEDLINE | ID: mdl-32168479

RESUMO

OBJECTIVE: Given the paucity of data on the natural history of brainstem cavernous malformations (CMs), the authors aimed to evaluate the annual hemorrhage rate and hemorrhagic risk of brainstem CMs. METHODS: Nine hundred seventy-nine patients diagnosed with brainstem CMs were referred to Beijing Tiantan Hospital from 2006 to 2015; 224 patients were excluded according to exclusion criteria, and 47 patients were lost to follow-up. Thus, this prospective observational cohort included 708 cases (324 females). All patients were registered, clinical data were recorded, and follow-up was completed. RESULTS: Six hundred ninety (97.5%) of the 708 patients had a prior hemorrhage, 514 (72.6%) had hemorrhagic presentation, and developmental venous anomaly (DVA) was observed in 241 cases (34.0%). Two hundred thirty-seven prospective hemorrhages occurred in 175 patients (24.7%) during 3400.2 total patient-years, yielding a prospective annual hemorrhage rate of 7.0% (95% CI 6.2%-7.9%), which decreased to 4.7% after the 1st year. Multivariate Cox regression analysis after adjusting for sex and age identified hemorrhagic presentation (HR 1.574, p = 0.022), DVA (HR 1.678, p = 0.001), mRS score ≥ 2 on admission (HR 1.379, p = 0.044), lesion size > 1.5 cm (HR 1.458, p = 0.026), crossing the axial midpoint (HR 1.446, p = 0.029), and superficially seated location (HR 1.307, p = 0.025) as independent adverse factors for prospective hemorrhage, but history of prior hemorrhage was not significant. The annual hemorrhage rates were 8.3% and 4.3% in patients with and without hemorrhagic presentation, respectively; the rate was 9.9%, 6.0%, and 1.0% in patients with ≥ 2, only 1, and 0 prior hemorrhages, respectively; and the rate was 9.2% in patients with both hemorrhagic presentation and focal neurological deficit on admission. CONCLUSIONS: The study reported an annual hemorrhage rate of 7.0% exclusively for brainstem CMs, which significantly increased if patients presented with both hemorrhagic presentation and focal neurological deficit (9.2%), or any other risk factor. Patients with a risk factor for hemorrhage needed close follow-up regardless of the number of prior hemorrhages. It should be noted that the referral bias in this study could have overestimated the annual hemorrhage rate. This study improved the understanding of the natural history of brainstem CMs, and the results are important for helping patients and physicians choose a suitable treatment option based on the risk factors and stratified annual rates.Clinical trial registration no.: ChiCTR-POC-17011575 (http://www.chictr.org.cn/).

2.
Int J Biol Sci ; 16(6): 957-969, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-32140065

RESUMO

Pregnancy constitutes a major challenge to the maternal immune system, which must tolerate fetal alloantigen encoded by paternal genes. In addition to their role in inducing maternal-fetal immune tolerance, accumulating evidence indicates that decidual immune cells are involved in several processes required for a successful pregnancy, including trophoblast invasion as well as tissue and spiral artery remodeling. Innate lymphoid cells (ILCs), an important branch of the innate immune system, which has expanded rapidly in recent years, are strong actors in mucosal immunity, tissue homeostasis and metabolism regulation. With the recent identification of ILCs in the human decidua, the role of ILCs at the maternal-fetal interface raises concern. Herein, we review the presence and characterization of ILCs in the human decidua, as well as their function in normal pregnancy and pathological pregnancy, including reproductive failure, preeclampsia and others.

3.
J Hazard Mater ; 393: 122329, 2020 Feb 19.
Artigo em Inglês | MEDLINE | ID: mdl-32126423

RESUMO

An efficient Sb-doped SnO2 electrode featuring superior electrocatalytic characteristic and long stability was constructed by adopting clustered TiO2 nanotubes-covered Ti mesh as substrate (M-TNTs-SnO2). Compared with the electrodes prepared with mere Ti mesh or Ti plate grew with TiO2 nanotube, the M-TNTs-SnO2 exhibited higher TOC removal (99.97 %) and mineralization current efficiency (44.0 %), and longer accelerated service lifetime of 105 h for electrochemical degradation of phenol. The enhanced performance was mainly ascribed to the introduction of mutually self-supported TiO2 nanotube clusters in different orientations. Such unique structure not only favored a compact and smooth surface of catalyst layer which improved the stability of electrode by reinforcing the binding force between substrate and catalyst layer, but also increased the loading capacity for catalysts, leading to 1.5-2.2 times higher of ·OH generation, the main active species for indirect electrochemical oxidation of phenol. Meanwhile, the transverse electron transfer from TiO2 nanotube to catalyst layer was possibly achieved to further prompt the generation of ·OH. This study may provide a feasible option to design of efficient electrodes for electrocatalytic degradation of organic pollutants.

4.
Sci Total Environ ; 722: 137876, 2020 Mar 11.
Artigo em Inglês | MEDLINE | ID: mdl-32208257

RESUMO

The phosphorus recovery efficiency in PAOs-biofilm system is so far limited to stimulating phosphorus release by adding concentrated organic carbon solution during the anaerobic stage. In present study, a PAOs-biofilm sequence batch reactor (BSBR) were operated to investigate whether increase of the phosphorus storage content via DO control can stimulate the release in the biofilm. During the operation of BSBR for 160 days, the phosphorus content in biofilm (Pbiofilm) was doubled via increasing dissolved oxygen (DO) from 4 to 6 mg/L. With the COD of 200 mg/L in the anaerobic phase, the phosphorus release was enhaced, resulting in an significant increase of phosphorus concentration from 94.85 to 179.5 mg/L in recirculated solution. Batch tests further clarified explicitly the increase of Pbiofilm stimulated a phosphorus release rate but this must be balanced since high Pbiofilm reduced the phosphorus removal capacity of the biofilm. With analysis of P31-NMR, Ortho-P and Poly-P were the main phosphorus species stored in biofilm. The microbial cell played a more important role than EPS in phosphorus storage. The dominant phylum in the master reactor was Proteobacteria with an abundance of 64.4%, in which the Rhodocyclaceae was the dominant PAOs with an abundance of 10.1%. The outcome of this study elucidated that increase of phosphorus storage via DO control which facilitated more efficient phosphorus release with much lower organic carbon source consumption during the enrichment of phosphorus. Meanwhile, we provided a new perspective for the recovery of phosphorus in future wastewater treatment plants.

5.
Sci Total Environ ; 723: 137995, 2020 Mar 17.
Artigo em Inglês | MEDLINE | ID: mdl-32213409

RESUMO

Coal gasification wastewater (CGW) contains several types of aromatic pollutants, which impart high biotoxicity and reduce the quality of anaerobic treatment. Two types of hydrolysis acidification processes, namely microaerobic hybrid reactor (HA-1) and upflow anaerobic sludge blanket reactor (HA-2), were developed for pre-treatment before the anaerobic treatment. The changes in the dissolved organic matter and biotoxicity were investigated to comprehensively understand the degradation process. The results showed that HA-2 coupled with an anaerobic reactor achieved a 12.3% and 13.4% higher removal efficiency for chemical oxygen demand and total phenols, respectively, compared with the coupled process with HA-1. Furthermore, HA-2 could transform macromolecules into small molecules more efficiently and produce fewer intermediates. The coupled process with HA-2 preferentially removed complex aromatic substances with absorption wavelengths of 285 and 254 nm, according to the sequential orders interpreted from two-dimensional correlation spectroscopy. In addition, the results of fluorescence excitation-emission-matrix with regional integration analysis revealed that the contents of typical cyclic compounds in CGW, such as phenolic, heterocyclic, and polycyclic aromatic compounds were remarkably reduced by HA-2. In addition, HA-2 reduced the toxic unit value of CGW by 67.5% and increased the resazurin dehydrogenase activity of the sludge by 37.5% during CGW treatment, thus improving the biotoxicity removal and biodegradability. However, the coupled process with HA-2 did not significantly affect the "indirect estrogenic activity" of CGW. A Pearson correlation analysis indicated that spectral indicators, such as UV254 and ΦT,n, presented a high positive correlation with the reduction of acute toxicity and organics.

6.
J Exp Med ; 217(5)2020 May 04.
Artigo em Inglês | MEDLINE | ID: mdl-32181805

RESUMO

Type I interferons (IFNs) are known to mediate antineoplastic effects during tumor progression. Type I IFNs can be produced by multiple cell types in the tumor microenvironment; however, the molecular mechanisms by which tumor cells evade the inhibition of immune microenvironment remain unknown. Here we demonstrate that glioma stem-like cells (GSCs) evade type I IFN suppression through downregulation of STAT1 to initiate tumor growth under inhospitable conditions. The downregulation of STAT1 is mediated by MBD3, an epigenetic regulator. MBD3 is preferentially expressed in GSCs and recruits NuRD complex to STAT1 promoter to suppress STAT1 expression by histone deacetylation. Importantly, STAT1 overexpression or MBD3 depletion induces p21 transcription, resensitizes GSCs to IFN suppression, attenuates GSC tumor growth, and prolongs animal survival. Our findings demonstrate that inactivation of STAT1 signaling by MBD3/NuRD provides GSCs with a survival advantage to escape type I IFN suppression, suggesting that targeting MBD3 may represent a promising therapeutic opportunity to compromise GSC tumorigenic potential.

7.
Artigo em Inglês | MEDLINE | ID: mdl-32182032

RESUMO

Si/C composites, combining the advantages of both carbon materials and Si materials, have been proposed as the promising material in lithium ion storage. However, up to now, the most common fabrication methods of Si/C composites are too complicated for practical application. Here, we first use phenyl-substituted cage-like polyhedral silsesquioxane (Tn-Ph, n=8, 12) as both carbon and silicon precursors to prepare the high-performance Si/C anode materials via a low-temperature and simple AlCl3-assisted magnesiothermic reduction. AlCl3 plays two roles in the reduction process, on the one hand, it acts as liquid medium to promote the reduction of siloxane core in such a mild condition (200 ℃), and on the other hand, it act as catalyst for phenyl groups polycondensation into carbon materials, which makes the procedure of fabrication feasible and controllable. Impressively, T12-Si/C exhibits an excellent lithium anodic performance with a reversible capacity of 1449.2 mA h g-1 with a low volume expansion of 16.3 % after 100 cycles. Such superior electrochemical performance makes the Si/C composites alternative anode materials for lithium ion battery.

8.
Reprod Biol Endocrinol ; 18(1): 18, 2020 Mar 09.
Artigo em Inglês | MEDLINE | ID: mdl-32164758

RESUMO

BACKGROUND: Polycystic ovarian syndrome (PCOS) is a multi-gene hereditary disorder caused by the interaction of certain gene variation with environmental factors. Previous studies have shown that vascular endothelial growth factor (VEGF) gene polymorphisms are associated with the risk of polycystic ovarian syndrome. However, the results of these studies remain controversial. We performed the present meta-analysis aiming to further investigate the potential relationship between VEGF polymorphisms and susceptibility to PCOS. METHODS: The following databases were systematically searched: PubMed, EMBASE, Web of Science (WOS), China National Knowledge Infrastructure (CNKI), and Wanfang Databases. The correlation between VEGF polymorphisms and PCOS risk was assessed by calculating pooled odds ratios (ORs) and their 95% confidence intervals (95% CIs). Subgroup analyses stratified by ethnicity and source of control were also conducted. Besides, trial sequential analysis (TSA) was done to verify the reliability of the pooled results. RESULTS: 10 relevant case-control studies were incorporated in this meta-analysis, involving 1347 PCOS cases and 1378 controls. The VEGF rs2010963 polymorphism was associated with decreased PCOS risk in the whole population and the Asian populations. The VEGF rs3025039 polymorphism was associated with decreased PCOS susceptibility and the Asian populations, but increased risk of PCOS was observed among the Caucasian populations. In addition, the results of trial sequential analysis (TSA) showed the negative correlation between rs2010963 and PCOS risk, obtained by our meta-analysis, was stable and reliable. CONCLUSION: Overall, different VEGF gene polymorphisms may exert different effects on PCOS susceptibility. The VEGF rs2010963 polymorphism decreases PCOS susceptibility in both the whole population and the Asian populations, and VEGF rs3025039 polymorphism causes lower PCOS susceptibility in the whole population and the Asian populations but higher in the Caucasian populations.

9.
Neurosurg Rev ; 2020 Mar 18.
Artigo em Inglês | MEDLINE | ID: mdl-32189095

RESUMO

Primary intracranial gliosarcoma is a rare malignant brain tumour, and the most effective treatment for gliosarcoma remains unclear. This study aimed to identify risk factors for progression-free survival (PFS) and overall survival (OS) in these cases. This retrospective single-centre study evaluated 103 patients (median age, 51 years; 67 men [65%]) with primary intracranial gliosarcoma between 2006 and 2017. Treatments included surgery (GTR, 63 patients; STR, 39 patients; biopsy, 1 patient), radiotherapy (adjuvant, 76 patients; exclusive treatment, 1 patient), and chemotherapy (adjuvant temozolomide, 52 patients; adjuvant nimustine/teniposide, 19 patients; adjuvant bevacizumab, 1 patient; exclusive nimustine/teniposide treatment, 1 patient). The median OS was 13.3 months, and the median PFS was 9.1 months. In the multivariate analyses, the poor prognostic factors were ependymal lining enhancement of the lateral ventricle (PFS, HR 2.406, p = 0.005; OS, HR 2.946, p = 0.009) and enhancement in the motor functional cortex (PFS, HR 2.892, p = 0.002; OS, HR 2.639, p = 0.009). Good OS was predicted by adjuvant radiotherapy alone (HR 0.071, p < 0.001), adjuvant temozolomide-based chemotherapy alone (HR 0.063, p = 0.005), adjuvant temozolomide-based chemotherapy with concurrent radiotherapy (HR 0.056, p < 0.001), and salvage surgery at recurrence (HR 0.449, p = 0.031). The present study revealed that, in patients with primary intracranial gliosarcoma, enhancement in the functional motor cortex and ependymal lining enhancement of the lateral ventricle were both poor prognostic factors. Survival was optimized in cases treated using maximal safe resection followed by adjuvant temozolomide-based chemotherapy with concurrent radiotherapy. Furthermore, salvage surgery provided meaningful therapeutic benefits for recurrent gliosarcoma.

10.
Sensors (Basel) ; 20(6)2020 Mar 18.
Artigo em Inglês | MEDLINE | ID: mdl-32197380

RESUMO

In recent years, wireless-based fingerprint positioning has attracted increasing research attention owing to its position-related features and applications in the Internet of Things (IoT). In this paper, by leveraging long-term evolution (LTE) signals, a novel deep-learning-based fingerprint positioning approach is proposed to solve the problem of outdoor positioning. Considering the outstanding performance of deep learning in image classification, LTE signal measurements are converted into location grayscale images to form a fingerprint database. In order to deal with the instability of LTE signals, prevent the gradient dispersion problem, and increase the robustness of the proposed deep neural network (DNN), the following methods are adopted: First, cross-entropy is used as the loss function of the DNN. Second, the learning rate of the proposed DNN is dynamically adjusted. Third, this paper adopted several data enhancement techniques. To find the best positioning fingerprint and method, three types of fingerprint and five positioning models are compared. Finally, by using a deep residual network (Resnet) and transfer learning, a hierarchical structure training method is proposed. The proposed Resnet is used to train with the united fingerprint image database to obtain a positioning model called a coarse localizer. By using the prior knowledge of the pretrained Resnet, feed-forward neural network (FFNN)-based transfer learning is used to train with the united fingerprint database to obtain a better positioning model, called a fine localizer. The experimental results convincingly show that the proposed DNN can automatically learn the location features of LTE signals and achieve satisfactory positioning accuracy in outdoor environments.

11.
Med Oncol ; 37(4): 33, 2020 Mar 21.
Artigo em Inglês | MEDLINE | ID: mdl-32200436

RESUMO

This study aims to identify the core modules associated with pancreatic cancer (PC) types and the ncRNAs and transcription factors (TFs) that regulate core module genes by weighted gene co-expression network analysis (WGCNA). WGCNA was used to analyze the union of genes related to PC in NCBI and OMIM databases and the differentially expressed genes screened by TCGA-PAAD database. Samples were clustered according to gene expression in gene modules and Fisher exact method was performed. GO and KEGG were used for enrichment analysis to visually display module genes and screen driver genes. Hypergeometric test method was used to calculate pivot nodes among ncRNAs, TFs and mRNA based on RAID 2.0 and TRRUST v2 databases. The blue and yellow modules were identified as the core modules associated with PC types. MST1R, TMPRSS, MIR198, SULF1, COL1A1 and FAP were the core genes in the modules. Hypergeometric test results showed that ANCR, miR-3134, MT1DP, LOC154449, LOC28329 and other ncRNAs were key factors driving blue module genes, while LINC-ROR, UCA1, SNORD114-4, HEIH, SNORD114-6 and other ncRNAs were key factors driving yellow module genes. TFs with significant regulatory effect on blue module included LCOR, PIAS4, ZEB1, SNAI2, SMARCA4, etc. and on yellow module included HOXC6, PER2, HOXD3, TWIST2, VHL, etc. The core modules associated with PC types were proved as yellow and blue modules, and important ncRNAs and TFs regulating yellow and blue modules were found. This study provides relevant evidence for further identification of PC types.

12.
Nucleic Acids Res ; 2020 Feb 29.
Artigo em Inglês | MEDLINE | ID: mdl-32112098

RESUMO

MORC family CW-type zinc finger 2 (MORC2) is an oncogenic chromatin-remodeling enzyme with an emerging role in DNA repair. Here, we report a novel function for MORC2 in cell-cycle checkpoint control through an acetylation-dependent mechanism. MORC2 is acetylated by the acetyltransferase NAT10 at lysine 767 (K767Ac) and this process is counteracted by the deacetylase SIRT2 under unperturbed conditions. DNA-damaging chemotherapeutic agents and ionizing radiation stimulate MORC2 K767Ac through enhancing the interaction between MORC2 and NAT10. Notably, acetylated MORC2 binds to histone H3 phosphorylation at threonine 11 (H3T11P) and is essential for DNA damage-induced reduction of H3T11P and transcriptional repression of its downstream target genes CDK1 and Cyclin B1, thus contributing to DNA damage-induced G2 checkpoint activation. Chemical inhibition or depletion of NAT10 or expression of an acetylation-defective MORC2 (K767R) forces cells to pass through G2 checkpoint, resulting in hypersensitivity to DNA-damaging agents. Moreover, MORC2 acetylation levels are associated with elevated NAT10 expression in clinical breast tumor samples. Together, these findings uncover a previously unrecognized role for MORC2 in regulating DNA damage-induced G2 checkpoint through NAT10-mediated acetylation and provide a potential therapeutic strategy to sensitize breast cancer cells to DNA-damaging chemotherapy and radiotherapy by targeting NAT10.

13.
Otolaryngol Head Neck Surg ; : 194599820904055, 2020 Mar 03.
Artigo em Inglês | MEDLINE | ID: mdl-32122245

RESUMO

OBJECTIVE: To describe our clinical experience with surgical treatments for sinonasal phosphaturic mesenchymal tumors diagnosed at our institution. STUDY DESIGN: Retrospective case series. SETTING: Affiliated Sixth People's Hospital, Shanghai Jiao Tong University. SUBJECTS AND METHODS: We retrospectively reviewed the medical records of 10 patients diagnosed with phosphaturic mesenchymal tumors associated with tumor-induced osteomalacia between December 2014 and October 2019. RESULTS: There were 4 men and 6 women with a disease course of 1 to 19 years. All patients exhibited hypophosphatemia and tumor-induced osteomalacia. The tumor was located in the sinonasal region, frontal bone, and temporal bone in 8 patients, 1 patient, and 1 patient, respectively. Technetium-99m octreotide scintigraphy was used for tumor localization in 4 cases. Six patients underwent endoscopic resection; the remaining 4 underwent unilateral transorbital anterior and posterior ethmoid artery ligation + endoscopic resection, endoscopic resection + skull base repair, internal carotid artery stenting + transcatheter arterial embolization + temporal bone tumor excision + adipose tissue plugging, and endoscopic resection + transfrontal craniotomy (n = 1 each). Two patients had a history of incomplete endoscopic resection. All patients achieved clinical remission and normalized biochemical indices after surgery. Only 1 patient developed recurrence and died of a brain hernia. CONCLUSIONS: A diagnosis of sinonasal phosphaturic mesenchymal tumors should be based on a combination of clinical, imaging, and pathological findings. Technetium-99m octreotide scintigraphy helps in locating the tumor. Complete surgical excision guarantees clinical remission, and preoperative transcatheter arterial embolization or feeding artery ligation may reduce intraoperative bleeding in cases of highly vascularized tumors.

14.
Phys Chem Chem Phys ; 22(9): 5242-5248, 2020 Mar 07.
Artigo em Inglês | MEDLINE | ID: mdl-32091052

RESUMO

High-pressure technology can help us to obtain excellent materials. We have explored alkaline-earth-metal strontium nitrides under different pressures, theoretically. A variety of stable Sr-N structures were predicted by the structure searching method using CALYPSO code. Six new stoichiometries, SrN, Sr2N3, SrN2, SrN3, SrN4, and SrN5, were predicted. And our calculation proved that all these compounds were stable existing under ambient pressure up to 100 GPa. A rich variety of poly-nitrogen forms appeared in the newly predicted SrNx compounds, including four nitrogen polymerization forms: ranging from N2, N3, N4, and N5 molecules, to zig-zag nitrogen chains and extended chains connected by puckered "N6" rings. Significantly, the 1D extended polymeric chain of puckered "N6" rings was firstly identified in the P1[combining macron]-SrN3 structure at 60 GPa. Another N-rich C2/c-SrN4 was stable only under the relatively high-pressure of 20 GPa, but this phase can be quenched under atmospheric pressure. The N-rich phase SrN5 maintained structural stability when the pressure reached 50-70 GPa. The delocalization of π electrons from N atoms was the principal cause for its metallicity in SrN5. In this paper, our calculated results indicated that the energetic poly-nitrides in alkaline-earth-metal nitrides can be obtained by the high-pressure method.

15.
Chem Commun (Camb) ; 56(19): 2933-2936, 2020 Mar 05.
Artigo em Inglês | MEDLINE | ID: mdl-32040106

RESUMO

We developed a novel enzyme-free amplified SERS immunoassay by combining silver nanoparticle (AgNP)-linked immunoreaction and SERS transduction for the detection of disease biomarkers. As a proof of concept, our method was successfully illustrated with the disease biomarker α-fetoprotein with the detection limit of 3.3 × 10-13 g mL-1 and a double-blind experiment consisting of tens of serum samples was performed to confirm its reliability.

16.
J Proteome Res ; 2020 Mar 04.
Artigo em Inglês | MEDLINE | ID: mdl-32073269

RESUMO

Accurate identification of lipids in biological samples is a key step in lipidomics studies. Multidimensional nuclear magnetic resonance (NMR) spectroscopy is a powerful analytical tool for this purpose as it provides comprehensive structural information on lipid composition at atomic resolution. However, the interpretation of NMR spectra of complex lipid mixtures is currently hampered by limited spectral resolution and the absence of a customized lipid NMR database along with user-friendly spectral analysis tools. We introduce a new two-dimensional (2D) NMR metabolite database "COLMAR Lipids" that was specifically curated for hydrophobic metabolites presently containing 501 compounds with accurate experimental 2D 13C-1H heteronuclear single quantum coherence (HSQC) chemical shift data measured in CDCl3. A new module in the public COLMAR suite of NMR web servers was developed for the (semi)automated analysis of complex lipidomics mixtures (http://spin.ccic.osu.edu/index.php/colmarm/index2). To obtain 2D HSQC spectra with the necessary high spectral resolution along both 13C and 1H dimensions, nonuniform sampling in combination with pure shift spectroscopy was applied allowing the extraction of an abundance of unique cross-peaks belonging to hydrophobic compounds in complex lipidomics mixtures. As shown here, this information is critical for the unambiguous identification of underlying lipid molecules by means of the new COLMAR Lipids web server, also in combination with mass spectrometry, as is demonstrated for Caco-2 cell and lung tissue cell extracts.

17.
Neurosurg Rev ; 2020 Feb 08.
Artigo em Inglês | MEDLINE | ID: mdl-32036505

RESUMO

Primary intracranial fibrosarcoma (PIF) was a rare tumor with a high relapse rate and dismal survival rate. This study aimed to delineate the clinical characteristics of primary intracranial fibrosarcoma (PIF) and the risk factors for outcomes. We reviewed 15 PIF patients, who underwent surgical treatment at our institution from January 2009 to December 2018. Meanwhile, 36 cases from the prior literature between November 1962 and December 2019 were also retrieved and pooled to identify the risk factors. In our cohort, while cystic component (46.7%), perilesional edema (83.3%), and vascular flow void (66.7%) were commonly observed, no patient was accurately diagnosed. The 2-year relapse-free survival (RFS) and overall survival (OS) were 12.2% and 30.2%, respectively. Based on the pooled data, tumor size (p = 0.006), Ki-67 index (p = 0.004), and radiotherapy dose (p = 0.029) were prognostic factors for RFS in univariate analysis. In the univariate analysis, tumor size (p = 0.002), NGTR (p = 0.049), and high Ki-67 index (p = 0.019) were significant predictors for OS; and further multivariate analysis (n = 18) showed that large tumor size (≥ 5 cm; HR 14.613, p = 0.022) and high Ki-67 index (≥ 30%; HR 5.879, p = 0.020) were the independent risk factors for OS. Due to the rarity and nonspecific clinicoradiological features, the correct diagnosis of PIF before surgery was challenging. The outcomes of PIF were poor, and GTR plus radiotherapy (at least 60 Gy) might benefit to the outcomes and were recommended. Future study with a large cohort was needed to verify our findings.

18.
Cell Rep ; 30(5): 1310-1318.e5, 2020 Feb 04.
Artigo em Inglês | MEDLINE | ID: mdl-32023451

RESUMO

Pathological activation of TGF-ß signaling is universal in fibrosis. Aberrant TGF-ß signaling in conjunction with transdifferentiation of hepatic stellate cells (HSCs) into fibrogenic myofibroblasts plays a central role in liver fibrosis. Here we report that the DNA demethylase TET3 is anomalously upregulated in fibrotic livers in both humans and mice. We demonstrate that in human HSCs, TET3 promotes profibrotic gene expression by upregulation of multiple key TGF-ß pathway genes, including TGFB1. TET3 binds to target gene promoters, inducing demethylation, which in turn facilitates chromatin remodeling and transcription. We also reveal a positive feedback loop between TGF-ß1 and TET3 in both HSCs and hepatocytes. Furthermore, TET3 knockdown ameliorates liver fibrosis in mice. Our results uncover a TET3/TGF-ß1 positive feedback loop as a crucial determinant of liver fibrosis and suggest that inhibiting TET3 may represent a therapeutic strategy for liver fibrosis and perhaps other fibrotic diseases.

19.
Artigo em Inglês | MEDLINE | ID: mdl-32100111

RESUMO

BACKGROUND: Ocular motor dysfunction is one of the most common postoperative complications of petroclival meningioma. However, its incidence, recovery rate, and independent risk factors remain poorly explored. METHODS: A prospective analysis of 31 petroclival meningiomas was performed. Operative approaches were selected by utilizing a new 6-region classification of petroclival meningiomas we proposed. Two scores were used to evaluate the functions of the oculomotor and abducens nerves. Pearson correlation analysis and binary logistic regression analysis were used to identify independent risk factors for intraoperative oculomotor and abducens nerve injury. RESULTS: Postoperative new-onset dysfunctions in the pupillary light reflex and eye/eyelid movements as well as abducens paralysis were detected in eight (25.8%), ten (32.3%) and twelve (38.7%) cases, respectively. Their corresponding recovery rates after 6 months of follow-up were 75% (6/8), 80% (8/10), and 83.3% (10/12), respectively, and their mean times to start recovery were 4.03, 2.43, and 2.5 months, respectively. Tumor invasion into the suprasellar region/sphenoid sinus was the only risk factor for dysfunctions in both the pupillary light reflex (p = 0.001) and eye/eyelid movements (p = 0.002). Intraoperative utilization of the infratrigeminal interspace was the only risk factor for dysfunction in eyeball abduction movement (p = 0.004). CONCLUSIONS: Dysfunctions of the oculomotor and abducens nerves recovered within 6 months postoperatively. Tumor extension into the suprasellar region/sphenoid sinus was the only risk factor for oculomotor nerve paralysis. Eye/eyelid movements were more sensitive than the pupillary light reflex in reflecting nerve dysfunctions. Intraoperative utilization of the infratrigeminal interspace was the only risk factor for abducens nerve paralysis.

20.
J Biomech Eng ; 2020 Feb 02.
Artigo em Inglês | MEDLINE | ID: mdl-32110795

RESUMO

AIMS: Central venous catheter (CVC) related thrombosis is a major cause of CVC dysfunction in patients under hemodialysis. Our study aims to investigate the influence of central venous catheter (CVC) insertion on the hemodynamic environment in the central veins and to see what hemodynamics changes due to the implantation of CVC might be related to thrombus formation. METHODS: Patient-specific models of the central veins before and after the insertion of CVC were rebuilt on the basis of computed tomography. Flow patterns in the vein were numerically simulated to obtain hemodynamic parameters including time-averaged wall shear stress (TAWSS), relative residence time (RRT),oscillating shear index (OSI) and normalized transWSS under pulsatile flow condition. RESULTS: The placement of CVC makes significant changes on the hemodynamic environment in the central veins. A great disturbance and increase of velocity can be observed in central veins after the insertion of CVC. As a result, TAWSS and transWSS were markedly increased, but most parts of OSI and RRT decreased. Comparing with the clinical data, it's revealed that thrombosis mainly occurred at sections with elevated normalized transWSS. CONCLUSIONS: It is concluded that the flow disturbance especially the flow multi-directionality induced by the CVC might be the decisive factor to initiate thrombosis after CVC implantation. Accordingly, approaches to reduce the flow disturbance in the CVC placement might help to restrain the thrombosis. More case study with pre-operative and post-operative modeling and clinical follow-up should be carried out to verify these findings.

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