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1.
Micromachines (Basel) ; 10(12)2019 Dec 11.
Artigo em Inglês | MEDLINE | ID: mdl-31835793

RESUMO

The clinical characteristics of excreted tumor cells can be found in the urine of bladder cancer patients, meaning the identification of tumor cells in urine can assist in bladder cancer diagnosis. The presence of white blood cells and epithelial cells in the urine interferes with the recognition of tumor cells. In this paper, a technique for detecting cancer cells in urine based on microfluidics provides a novel approach to bladder cancer diagnosis. The bladder cancer cell line (T24) and MeT-5A were used as positive bladder tumor cells and non-tumor cells, respectively. The practicality of the tumor cell detection system based on microfluidic cell chip detection technology is discussed. The tumor cell (T24) concentration was around 1 × 104 to 300 × 104 cells/mL. When phosphate buffer saline (PBS) was the diluted solution, the tumor cell detected rate was 63-71% and the detection of tumor cell number stability (coefficient of variation, CV%) was 6.7-4.1%, while when urine was the diluted solution, the tumor cell detected rate was 64-72% and the detection of tumor cell number stability (CV%) was 6.3-3.9%. In addition, both PBS and urine are tumor cell dilution fluid solutions. The sample was analyzed at a speed of 750 microns per hour. Based on the above experiments, a system for detecting bladder cancer cells in urine by microfluidic analysis chip technology was reported. The rate of recognizing bladder cancer cells reached 68.4%, and the speed reached 2 mL/h.

2.
Technol Health Care ; 27(4): 373-387, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-30664515

RESUMO

BACKGROUND: Facial expression recognition plays an essential role in affective computing, mental illness diagnosis and rehabilitation. Therefore, facial expression recognition has attracted more and more attention over the years. OBJECTIVE: The goal of this paper was to improve the accuracy of the Electroencephalogram (EEG)-based facial expression recognition. METHODS: In this paper, we proposed a fusion facial expression recognition method based on EEG and facial landmark localization. The EEG signal processing and facial landmark localization are the two key parts. The raw EEG signals is preprocessed by discrete wavelet transform (DWT). The energy feature vector is composed of energy features of the reconstructed signal. For facial landmark localization, images of the subjects' facial expression are processed by facial landmark localization, and the facial features are calculated by landmarks of essence. In this research, we fused the energy feature vector and facial feature vector, and classified the fusion feature vector with the support vector machine (SVM). RESULTS: From the experiments, we found that the accuracy of facial expression recognition was increased 4.16% by fusion method (86.94 ± 4.35%) than EEG-based facial expression recognition (82.78 ± 5.78%). CONCLUSION: The proposed method obtain a higher accuracy and a stronger generalization capability.

3.
Soft Matter ; 14(21): 4374-4379, 2018 May 30.
Artigo em Inglês | MEDLINE | ID: mdl-29767187

RESUMO

Here, we report separately enhanced dual emissions of the amphiphilic derivative of 2-(2'-hydroxyphenyl)benzothiazole (denoted as HBT-11) by supramolecular complexation with cyclodextrins (CDs). When dispersed in water, HBT-11 shows two relatively weak emission bands, which can be assigned to the emissions of enol- and keto-forms, the two tautomers, owing to excited-state intramolecular proton transfers. Upon the addition of α-CD and ß-CD, the keto- and enol-emissions, respectively, are separately enhanced; the enhancement effect is due to the formation of HBT-11/α-CD and HBT-11/ß-CD complexes through multiple hydrogen bonding and host-guest interactions, respectively. It is worth to note that the keto-emission caused by the complex of HBT-11/α-CD has a much shorter wavelength compared with that of the aggregates formed by pure HBT-11. To the best of our knowledge, this is the first time that a study on keto-emission of the isolated HBT chromophore has been reported.

4.
Soft Matter ; 14(21): 4231-4237, 2018 May 30.
Artigo em Inglês | MEDLINE | ID: mdl-29624193

RESUMO

3-Hydroxyflavone derivatives have great potential as fluorescent probes for bio-labeling in aqueous medium. They were extensively studied in various organic solvents for the "excited state intramolecular proton transfer" process, but seldom addressed in aqueous solution due to the poor water solubility. Herein, an amphiphilic molecule bearing 3-hydroxyflavone and oligo(ethylene oxide) (denoted as 3HF-EO) was designed and synthesized. Different from the fluorescence in organic solvents, 3HF-EO in aqueous solution showed a remarkable single fluorescence emission, which is ascribed to the fluorescence of its anionic species. We found that the fluorescence intensity could be efficiently tuned via host-guest complexation. α-CD has little effect on the emission, while ß-CD and γ-CD lead to enhanced and reduced emissions of 3HF-EO, respectively. The 1H NMR and 2D NOESY NMR spectra indicate that α-CD barely had any interaction with 3HF-EO, while ß-CD and γ-CD formed complexes with one and two 3HF-EO molecules, respectively. These results provide a sound explanation for the modulated fluorescence intensity.

5.
ACS Appl Mater Interfaces ; 9(31): 26234-26241, 2017 Aug 09.
Artigo em Inglês | MEDLINE | ID: mdl-28726369

RESUMO

Owing to the high charge mobility and low processing temperature, ZnO is regarded as an ideal candidate for electron transport layer (ETL) material in thin-film solar cells. For the film preparation, the presently dominated sol-gel (SG) and hydrolysis-condensation (HC) methods show great potential; however, the effect of these two methods on the performance of the resulting devices has not been investigated in the same frame. In this study, the ZnO films made through SG and HC methods were applied in perovskite solar cells (Pero-SCs), and the performances of corresponding devices were compared under parallel conditions. We found that the surface morphologies and the conductivities of the films prepared by SG and HC methods showed great differences. The HC-ZnO films with higher conductivity led to relatively higher device performance, and the best power conversion efficiencie (PCE) of 12.9% was obtained; meanwhile, for Pero-SCs based on SG-ZnO, the best PCE achieved was 10.9%. The better device performance of Pero-SCs based on HC-ZnO should be attributed to the better charge extraction and transportation ability of HC-ZnO film. Moreover, to further enhance the performance of Pero-SCs, a thin layer of pristine C60 was introduced between HC-ZnO and perovskite layers. By doing so, the quality of perovskite films was improved, and the PCE was elevated to 14.1%. The preparation of HC-ZnO film involves relatively lower-temperature (maximum 100 °C) processing; the films showed better charge extraction and transportation properties and can be a more promising ETL material in Pero-SCs.

6.
Langmuir ; 33(35): 8679-8685, 2017 09 05.
Artigo em Inglês | MEDLINE | ID: mdl-28640627

RESUMO

To provide orthogonal solvent processable surface modification and improve the device stability of bulk-heterojunction polymer solar cells (PSCs), n-type semiconducting material naphthalene diimide (NDI) was chemically introduced onto the ITO surface as a cathode interlayer (CIL) using 3-bromopropyltrimethoxysilane (BrTMS) as a coupling agent. After modification, the work function of ITO can be decreased from 4.70 to 4.23 eV. The modified ITO cathode was applied in inverted PSCs based on PTB7-Th:PC71BM. With the CIL modification, a champion power conversion efficiency (PCE) of 5.87% was achieved, showing a dramatic improvement compared to that of devices (PCE = 3.58%) without CIL. More importantly, with these chemical bonded interlayers, the stability of inverted PSCs was greatly enhanced. The improved PCE and stability can be attributed to the increased open-circuit voltage and the formation of robust chemical bonds in NDI-TMS films, respectively. This study demonstrated that chemical modification of ITO with n-type semiconducting materials provides an avenue for not only solving the solvent orthogonal problem but also improving the device performance in terms of the PCE and the stability.

7.
Huan Jing Ke Xue ; 29(10): 2894-8, 2008 Oct.
Artigo em Chinês | MEDLINE | ID: mdl-19143391

RESUMO

A long-term mining activity of Jinding Pb-Zn mine area in Yunnan has caused the serious cadmium pollution to the river sediments of the ambient area. The Cd contents of Beidagou river sediments (mean value <10 mg x kg(-1)) are lower than those of Nandagou river sediments (mean value = 266 mg x kg(-1)). The Cd contents of river sediments in different section of Bijiang river occur distinct difference. The Cd contents are rather lower in upstream sediments of Bijiang river, and increase substantially along downstream contaminated section of Bijiang river. The Cd pollution coefficient is higher (R = 21.9-45.2) than Zn (R = 4.9-9.7) and Pb (R = 2.7-4.6). The degree of pollution is estimated by the geoaccumulation index, which indicates the following rank of pollution elements: Cd > Zn > Pb. The river sediments are extremely contaminated in Nandagou river and downstream section of Bijiang river from Nandagou, strongly contaminated in most part of Beidagou river, moderately to strongly contaminated in few river section of Nandagou river, uncontaminated or moderately contaminated in upstream of Bijiang river from Jinding. The heavy metals pollution of river sediments presents increasing trend from Nandagou outlet to Wenzhuang section of Bijiang river.


Assuntos
Cádmio/análise , Sedimentos Geológicos/química , Chumbo , Mineração , Poluentes Químicos da Água/análise , Zinco , China , Rios
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