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1.
J Cell Physiol ; 235(1): 494-503, 2020 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-31236962

##### RESUMO

Dilated cardiomyopathy (DCM) is a heart disease that injured greatly to the people wordwide. Systemic co-expression analysis for this cancer is still limited, although massive clinic experiments and gene profiling analyses had been well performed previously. Here, using the public RNA-Seq data "GSE116250" and gene annotation of Ensembl database, we built the co-expression modules for DCM by Weighted Gene Co-Expression Network Analysis, and investigated the function enrichment and protein-protein interaction (PPI) network of co-expression genes of each module by Database for Annotation, Visualization, and Integrated Discovery and Search Tool for the Retrieval of Interacting Genes/Proteins database, respectively. First, 5,000 genes in the 37 samples were screened and 11 co-expression modules were conducted. The number of genes for each module ranged from 77 to 936, with a mean of 455. Second, interaction relationships of hub-genes between pairwise modules showed great differences, suggesting relatively high-scale independence of the modules. Third, functional enrichments of the co-expression modules exhibited great differences. We found that genes in module 3 were significantly enriched in the pathways of focal adhesion and ubiquitin-mediated proteolysis. This module was inferred as the key module involved in DCM. In addition, PPI analysis revealed that the genes HSP90AA1, CTNNB1, MAPK1, GART, and PPP2CA owned the largest number of adjacency genes, unveiling that they may function importantly during the occurrence of DCM. Focal adhesion and ubiquitin-mediated proteolysis play important roles in human DCM.

2.
Talanta ; 207: 120346, 2020 Jan 15.
Artigo em Inglês | MEDLINE | ID: mdl-31594588

##### RESUMO

Acute myocardial infarction (AMI) causes significant mortality and morbidity. The determination of multiple AMI biomarkers is very important for the timely diagnosis of AMI. In this work, simultaneous determination of three AMI biomarkers were achieved by virtue of a three-dimensional (3D) microfluidic paper-analytical device (µPAD) with temporally resolved chemiluminescence (CL) emissions for the first time. A dual-signal amplification strategy was introduced including by employing primary antibody functionalized gold nanoparticles (Ab1-GNPs) immobilized on the detection zone as amplified capture probes, and Co(II) catalyst, secondary antibody, luminol multifunctionalized gold nanoparticles (Co(II)-Ab2-luminol-GNPs) with excellent CL activity as amplified signal probes. CL immunoreactions were performed at three detection zone of the fabricated 3D µPAD by assembling Ab1-GNPs, antigen, and Co(II)-Ab2-luminol-GNPs to form sandwich-type immunocomplexes. Auto separated CL signals with temporal resolution were obtained by time delayed transport of H2O2 to different detection zones for multiplexed analysis. The CL signal obtained by using Co(II)-Ab2-luminol-GNPs as signal probe (10576 a.u.) were about 20-fold higher than that by using conventional horseradish peroxidase labeled antibody modified luminol-GNPs as signal probe (531 a.u.). Finally, three AMI biomarkers including heart-type fatty acid-binding protein (H-FABP), cardiac troponin I (cTnI) and copeptin were quantitatively analyzed in one CL detection run by reading the CL intensity of the obtained three CL emission peaks. The detection range were ultra-wide ranged from 0.1â¯pg/mL to 1â¯µg/mL, 0.5â¯pg/mL to 1â¯µg/mL and 1â¯pg/mL to 1â¯mg/mL with the detection limits down to 0.06â¯pg/mL, 0.3â¯pg/mL and 0.4â¯pg/mL for H-FABP, cTnI and copeptin detection, respectively. The developed µPAD based immunoassay performing multiplexed analysis ability, high sensitivity, ultra-wide dynamic range, favorable selectivity, accessible accuracy and reproducibility, have great application potential for the early diagnosis of AMI.

3.
Ecotoxicol Environ Saf ; 188: 109930, 2020 Jan 30.
Artigo em Inglês | MEDLINE | ID: mdl-31727496

##### RESUMO

BACKGROUND: For the sake of children's health, iodized salt supply has been stopped in many areas with excessive iodine in the drinking water, but children's iodine nutrition status and thyroid function after terminating the iodized salt supply is unknown. Objective We assessed the iodine nutrition, thyroid function and influencing factors for thyroid abnormalities in children from areas with different concentrations of water iodine; the supply of iodized salt has been stopped in high water iodine areas. This study aimed to evaluate whether the strategy of stopping the supplies of iodized salt alone is enough to avoid thyroid dysfunction in all areas with excess water iodine while still meeting the iodine nutrition needs of children. METHODS: A cross-sectional study was conducted in children from four areas with different drinking water iodine concentrations in Tianjin, China. The drinking water samplings and spot urine samples were collected to estimate the external and internal iodine exposure levels. The thyroid volume was measured, and blood samples were collected to assess thyroid function. Logistic regression analysis was used to analyze risk factors for thyroid abnormalities. A dietary survey was conducted to determine the sources of iodine nutrition among the areas with different iodine concentrations in the drinking water. RESULTS: In the area with a drinking water iodine concentration ≥300â¯µg/L, the median urinary iodine concentration (UIC) in children was 476.30 (332.20-639.30) µg/L, which was higher than that in other groups (all Pâ¯<â¯0.05), and the prevalence of thyroid nodules and the thyroid goiter rate were higher than those in the <100â¯µg/L, 100-150â¯µg/L and 150-300â¯µg/L areas (all Pâ¯<â¯0.01). Binary logistic regression analysis indicated that the risk of thyroid abnormalities was significantly increased in the UIC 200-299â¯µg/L group (OR: 4.534; 95% CI: 1.565, 13.135; bootstrapped 95% CI: 1.689, 21.206, Pâ¯=â¯0.004) and in the UICâ¯≥â¯300â¯µg/L group (OR: 6.962; 95% CI: 2.490, 19.460; bootstrapped 95% CI: 2.838, 32.570, Pâ¯=â¯0.001) compared to the 100-199â¯µg/L group. The iodine contribution rates from water in areas with water iodine concentrations ≥300â¯µg/L are up to 63.04%. CONCLUSIONS: After termination of the iodized salt supply, the level of iodine nutrition of children in the area with drinking water iodine concentrations ≥300â¯µg/L is still excessive. The water source needs to be replaced in this area. In the area with a water iodine concentration of 150-300â¯µg/L, it is proposed that stopping the supply of iodized salt is sufficient to achieve the proper iodine nutrition status in children.

4.
Clin Exp Immunol ; 2019 Dec 02.
Artigo em Inglês | MEDLINE | ID: mdl-31797350

##### RESUMO

Neopterin is primarily synthesized and released by activated macrophages/monocytes upon stimulation with interferon-Î³ and is considered as a marker for macrophage activation. This study aimed to analyze the serum levels of neopterin in patients with dermatomyositis (DM) in association with clinical manifestations, laboratory data and patient prognosis. One hundred and eighty-two consecutive DM patients and 30 healthy controls were retrospectively enrolled in the study. Serum levels of neopterin were significantly increased in DM patients compared to healthy controls (P<0.001). High serum neopterin levels were associated with anti-MDA5 antibody, rapidly progressive interstitial lung disease (RP-ILD), and characteristic DM cutaneous involvement. Longitudinal assessment of serum samples revealed that the serum neopterin levels were closely correlated with disease severity (ß=30.24, P<0.001). In addition, a significant increase in serum neopterin concentration of non-survivors was observed when compared to that of survivors (P<0.001). ROC curves showed that serum neopterin could distinguish non-survivors and survivors at an optimal cut-off level of 22.1 nmol/L with a sensitivity and specificity of 0.804 and 0.625 respectively (P<0.001). Kaplan-Meier survival curves revealed that DM patients with serum neopterin > 22.1 nmol/L had a significantly higher mortality compared to patient group with serum neopterin <22.1 nmol/L (logrank P<0.001). Multivariate regression analysis identified high serum neopterin concentration to be an independent risk factor for poor prognosis in DM (adjusted HR=4.619, 95%CI: 2.092-10.195, P<0.001). In conclusions, increased serum levels of neopterin were significantly associated with RP-ILD and reduced survival in DM patients, suggesting it as a promising biomarker in disease evaluation of DM.

5.
Aging (Albany NY) ; 11(22): 10781-10782, 2019 Nov 30.
Artigo em Inglês | MEDLINE | ID: mdl-31801905
6.
Math Biosci Eng ; 16(6): 7789-7807, 2019 Aug 26.
Artigo em Inglês | MEDLINE | ID: mdl-31698640

##### RESUMO

Entity prediction is the task of predicting a missing entity that has a specific relation-ship with another given entity. Researchers usually use knowledge graphs embedding(KGE) methods to embed triples into continuous vectors for computation and perform the tasks of entity prediction. However, KGE models tend to use simple operations to refactor entities and relationships, resulting in insufficient interaction of components of knowledge graphs (KGs), thus limiting the performance of the entity prediction model. In this paper, we propose a new entity prediction model called FRS(Feature Refactoring Scoring) to alleviate the problem of insufficient interaction and solve information incom-pleteness problems in the KGs. Different from the traditional KGE methods of directly using simple operations, the FRS model innovatively provides the procedure of feature processing in the entity prediction tasks, realizing the alignment of entities and relationships in the same feature space and improving the performance of entity prediction model. Although FRS is a simple three-layer network, we find that our own model outperforms state-of-the-art KGC methods in FB15K and WN18. Through extensive experiments on FRS, we discover several insights. For example, the effect of embedding size and negative candidate sampling probability on experimental results is in reverse.

7.
Environ Int ; : 105000, 2019 Nov 04.
Artigo em Inglês | MEDLINE | ID: mdl-31699440

##### RESUMO

Inhalation from ambient air and cigarette smoke is a common route of human exposure to polycyclic aromatic hydrocarbons (PAHs). Little information is available regarding hepatotoxicities of inhaled PAHs so for. In this study, we evaluated the toxic effects of intratracheally instilled benzo[a]pyrene (B[a]P) on hepatic lipid metabolism of C57BL/6 mice at relevant environmental exposure levels by using two different mass-based lipidomics approaches. The results of mass spectrometry imaging analysis showed that both the abundance and spatial distribution of several lysophosphatidylcholine (LysoPC), phosphatidylcholine (PC) and sphingomyelin (SM) in the liver section were different and changed after inhalation exposure to B[a]P. Liquid chromatography coupled with mass spectrometry-based lipidomics analysis and multivariate statistical analysis found that B[a]P exposure markedly altered glycerophospholipids, glycerolipids, and fatty acid metabolism in the mouse liver, with increasing of triacylglycerol (TG), phosphatidylinositol (PI) and PC, and decreasing of LysoPCs phosphatidylethanolamines (PEs), lysophosphatidylethanolamine (LysoPEs), free fatty acids (FFAs) and eicosanoids. B[a]P-induced lipid metabolic disorders showed a time-dependent effect, which generated three response trajectories with different change trends. Consequently, B[a]P exposure induced alteration of hepatic lipids by promoting the uptake from blood or the biosynthesis and transformation in the liver, might contribute to non-alcoholic fatty liver disease, hepatocyte membrane injury, inflammation, and signal system disturbance.

8.
J Ethnopharmacol ; : 112355, 2019 Oct 25.
Artigo em Inglês | MEDLINE | ID: mdl-31669667

##### RESUMO

ETHNOPHARMACOLOGICAL RELEVANCE: Thalictrum minus L., a Mongolian folk medicinal plant, was applied for the treatment of bacterial and fungal infection, tuberculosis and lung inflammation. AIM OF THE STUDY: The present work aims to elucidate the protective effects of Thalictrum minus L.(TML) against lipopolysaccharide (LPS)-induced acute lung injury and the underlying mechanisms. METHODS: The mice model of acute lung injury was induced by LPS via endotracheal drip, and TML (10, 20, 40â¯mg/kg) were administered orally 1â¯h prior to LPS. The efficacy and molecular mechanisms in the presence or absence of TML were investigated. RESULTS: We demonstrated that treatment with TML aqueous extract protected the mice from acute lung injury induced by LPS administration. TML significantly inhibited weight loss in mice, decreased the lung wet to dry weight (W/D) ratios and attenuated lung histopathological changes, such as infiltration of inflammatory cells and coagulation, pulmonary edema. Furthermore, we found that TML markedly reduced the LPS-induced inflammatory cytokines including tumor necrosis factor-α (TNF-α) and interleukin-1ß (IL-1ß), decreased nitric oxide (NO), and increased superoxide dismutase (SOD) in bronchoalveolar lavage fluid (BALF), and effectively ameliorated LPS-induced increased total protein, leukocyte and macrophages in BALF. In addition, TML pronouncedly suppressed the activation of the MAPKs p38-NLRP3/caspase-1 and COX2, increased the expression of p-AMPK-Nrf2, and suppressed the expression of KEAP, apoptotic-related protein as well as autophagy. CONCLUSIONS: These results suggested that TML ameliorated LPS-induced acute lung injury by inhibiting the release of inflammatory cytokines and reducing oxidative damage associated with the MAPKs p38-NLRP3/caspase-1 and COX2 signaling pathways, AMPK-Nrf2/KEAP signaling pathways, as well as apoptosis and autophagy.

9.
BMC Bioinformatics ; 20(1): 553, 2019 Nov 06.
Artigo em Inglês | MEDLINE | ID: mdl-31694521

##### RESUMO

BACKGROUND: Tea is the oldest and among the world's most popular non-alcoholic beverages, which has important economic, health and cultural values. Tea is commonly produced from the leaves of tea plants (Camellia sinensis), which belong to the genus Camellia of family Theaceae. In the last decade, many studies have generated the transcriptomes of tea plants at different developmental stages or under abiotic and/or biotic stresses to investigate the genetic basis of secondary metabolites that determine tea quality. However, these results exhibited large differences, particularly in the total number of reconstructed transcripts and the quality of the assembled transcriptomes. These differences largely result from limited knowledge regarding the optimized sequencing depth and assembler for transcriptome assembly of structurally complex plant species genomes. RESULTS: We employed different amounts of RNA-sequencing data, ranging from 4 to 84 Gb, to assemble the tea plant transcriptome using five well-known and representative transcript assemblers. Although the total number of assembled transcripts increased with increasing sequencing data, the proportion of unassembled transcripts became saturated as revealed by plant BUSCO datasets. Among the five representative assemblers, the Bridger package shows the best performance in both assembly completeness and accuracy as evaluated by the BUSCO datasets and genome alignment. In addition, we showed that Bridger and BinPacker harbored the shortest runtimes followed by SOAPdenovo and Trans-ABySS. CONCLUSIONS: The present study compares the performance of five representative transcript assemblers and investigates the key factors that affect the assembly quality of the transcriptome of the tea plants. This study will be of significance in helping the tea research community obtain better sequencing and assembly of tea plant transcriptomes under conditions of interest and may thus help to answer major biological questions currently facing the tea industry.

10.
Org Lett ; 21(22): 9276-9279, 2019 Nov 15.
Artigo em Inglês | MEDLINE | ID: mdl-31686514

##### RESUMO

An efficient in situ Pd-NHC catalytic system for regioselective arylation of B(3,6)-H bonds of o-carborane has been developed for the first time. A series of symmetric and unsymmetric 3,6-diaryl-o-carboranes anchored with active groups have been synthesized with moderate to good yields under mild conditions. This work offers an efficient protocol for selective activation of B(3,6)-H bonds and has important value in design coupling reactions for selective functionalization of o-carboranes.

11.
Clin Nucl Med ; 44(12): 998-1000, 2019 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-31689285

##### RESUMO

A 39-year-old previously healthy woman presented possible hematuria. An ultrasound examination showed right adrenal mass and suggested pheochromocytoma. A Tc-HYNIC-TOC SPECT/CT was performed, which incidentally detected situs inversus totalis and suspicion of polysplenia without definite normal spleen in the right upper abdomen. In order to differentiate the polysplenia from other etiologies, a heat-damaged Tc RBC scintigraphy was performed. The images showed significant activity in the multiple soft tissue nodules in the posterior right abdomen, consistent with ectopic polysplenia.

12.
BMC Cancer ; 19(1): 1123, 2019 Nov 19.
Artigo em Inglês | MEDLINE | ID: mdl-31744467

##### RESUMO

BACKGROUND: The rs2057482 polymorphism in the hypoxia inducible factor 1 subunit alpha (HIF1A) gene has been reported to be associated with a risk of several types of cancer, but this association has not yet been definitively confirmed. We performed this meta-analysis to determine whether rs2057482 is associated with overall cancer risk. METHODS: The PubMed, Embase, and Web of Science databases were searched for the potential studies about the association between the rs2057482 and cancer risk. The data of genotype frequencies in cases with cancer and controls were extracted from the selected studies. Odds ratios (ORs) and the corresponding 95% confidence intervals (CIs) were calculated to determine the strength of the associations. RESULTS: The meta-analysis showed an association between the rs2057482 polymorphism and overall cancer risk. However, a stratified analysis of ethnicity did not show any significant association between rs2057482 and cancer risk in the Asian population. CONCLUSIONS: The rs2057482 polymorphism was associated with decreased overall cancer risk, based on the currently available studies. However, this conclusion needs verification by further well-designed epidemiology studies that examine different cancer types and more subjects.

13.
Mol Med Rep ; 2019 Nov 20.
Artigo em Inglês | MEDLINE | ID: mdl-31746411

##### RESUMO

Wilson disease (WD) is a rare autosomal recessive genetic disorder that causes abnormal copper metabolism, resulting in pathological accumulation of copper in the liver, brain and other organs. Mutations in the ATPase copper transporter 7B (ATP7B) gene, which encodes a membrane P­type adenosine triphosphatase, have been identified as being responsible for WD. The present study analyzed clinical data and collected DNA samples from a pediatric patient with WD and her healthy parents. Mutation screening for ATP7B was performed using direct sequencing, multiplex ligation­dependent probe amplification(MLPA), next­generation sequencing (NGS) and Sanger sequencing of the breakpoint junction sequence. The patient (age, 2.7 years) presented with early­onset hepatic disease. The present study identified compound heterozygous mutations of ATP7B, including a heterozygous mutation (p.Arg1,041Trp) and a novel heterozygous gross deletion of a 57,771 bp fragment (chr13: 52490972­52548742) (GRCh37) from partial exon2­ exon21 to external ATP7B sequence (15.833bp) in the patient. Analysis of the family members of the patient showed that the missense mutation and the gross deletion mutation were inherited from her mother and father, respectively. Microhomology and inverted repeat sequences, which may mediate the deletion mutation, were identified through sequence analysis on both sides of the breakpoints of this deletion. The present study provided additional information on the genotypic spectrum of the ATP7B gene, particularly with regard to early onset hepatic disease, as observed in the present patient with WD. The identification of the precise breakpoint junction sequence warrants further investigation of DNA break and recombination mechanisms. In detecting precise deletions, the NGS associated with Sanger sequencing of breakpoint junction sequence have been found to have more advantages than MLPA.

14.
J Med Virol ; 2019 Oct 31.
Artigo em Inglês | MEDLINE | ID: mdl-31670402

##### RESUMO

Human papillomavirus (HPV)16 gene mutation is usually associated with persistent HPV infection and cervical intraepithelial neoplasia (CIN). However, the functional implications of HPV16 mutations remain poorly understood.145 LCR/E6/E7 of the HPV16 isolates were amplified and sequenced, and HPV16 integration status was detected. In total, 89 SNPs (68 in the LCR, 13 in E6, 8 in E7) were discovered, 11 of which were nonsynonymous mutations (8 in E6, 3 in E7). The H85Y and E120D variants in E6 were significantly reduced in the high-grade squamous intraepithelial lesion (HSIL) group compared to the T," a potential binding site for TATA-binding protein, is the most common in LCR variants. A4 (Asian) was associated with an increased risk of HSIL compared to A1-3(P = .009). The H85/E120 in E6 and N29 in HPV16 E7 might play a critical role in carcinogenesis by disrupting p53 and Rb degradation due to affecting their interaction, respectively. In a word, the findings in this study provide preventative and therapeutic interventions of HPV16 -related cervical lesions/cancer.

15.
Appl Opt ; 58(30): 8290-8294, 2019 Oct 20.
Artigo em Inglês | MEDLINE | ID: mdl-31674501

##### RESUMO

This study proposes a new method for the preparation of novel MXenes/metal composites with unique chemical properties using reducing agent action. The effect of the reduction reaction time on the preparation conditions of ${{\rm Ti}_3}{{\rm C}_2}{{\rm T}_x}/{\rm Au}$Ti3C2Tx/Au nanocomposites is studied, and the contribution of the prepared nanocomposites to the surface Raman reinforcement is analyzed. This study further explores the application of MXene in surface-enhanced Raman spectroscopy.

16.
Clin Nucl Med ; 2019 Oct 23.
Artigo em Inglês | MEDLINE | ID: mdl-31693628

##### RESUMO

A 39-year-old previously healthy woman presented possible hematuria. An ultrasound examination showed right adrenal mass and suggested pheochromocytoma. A Tc-HYNIC-TOC SPECT/CT was performed, which incidentally detected situs inversus totalis and suspicion of polysplenia without definite normal spleen in the right upper abdomen. In order to differentiate the polysplenia from other etiologies, a heat-damaged Tc RBC scintigraphy was performed. The images showed significant activity in the multiple soft tissue nodules in the posterior right abdomen, consistent with ectopic polysplenia.

17.
Cancer Lett ; 2019 Nov 12.
Artigo em Inglês | MEDLINE | ID: mdl-31730903

##### RESUMO

The immune cells within the tumor microenvironment (TME) play important roles in tumorigenesis. It has been known that these tumor associated immune cells may possess tumor-antagonizing or tumor-promoting functions. Although the tumor-antagonizing immune cells within TME tend to target and kill the cancer cells in the early stage of tumorigenesis, the cancer cells seems to eventually escape from immune surveillance and even inhibit the cytotoxic function of tumor-antagonizing immune cells through a variety of mechanisms. The immune evasion capability, as a new hallmark of cancer, accidently provides opportunities for new strategies of cancer therapy, namely harnessing the immune cells to battle the cancer cells. Recently, the administrations of immune checkpoint modulators (represented by anti-CTLA4 and anti-PD antibodies) and adoptive immune cells (represented by CAR-T) have exhibited unexpected antitumor effect in multiple types of cancer, bringing a new era for cancer therapy. Here, we review the biological functions of immune cells within TME and their roles in cancer immunotherapy, and discuss the perspectives of the basic studies for improving the effectiveness of the clinical use.

18.
Sci Total Environ ; : 135514, 2019 Nov 20.
Artigo em Inglês | MEDLINE | ID: mdl-31780164

##### RESUMO

Compared with phosphate (+5 valence), phosphite (HPO32-/H2PO3-, +3 valence) possesses higher solubility, and is more resistant to biotransformation. Herein, we designed a one-step electroactive filter technology for rapid and efficient phosphite removal. The filter consists of carbon nanotubes (CNT) and functionalized with nanoscale TiCe binary oxides. The phosphite removal kinetics and capacity increased with electric field (e.g., from 54.5% at 0 V to 75.6% at 2 V) and flow rate (e.g., from 63.1% at 1.5 mL/min to 81.2% at 6 mL/min). This can be attributed to synergistic effects of the filter's electrochemical reactivity, limited pore size, more exposed active sites and flow-through design. Meanwhile, phosphite can be converted to phosphate once adsorbed under electric field. The TiO2/CeOx-CNT filter could work effectively across a wide pH range, and the presence of various coexisting anions posed negligible impact on phosphite removal. Electrochemical characterizations verified the essential role of CeOx and applied electric field, which synergistically accelerated electron transfer rate and increased charge capacity. The TiO2/CeOx-CNT filter can be regenerated effectively by chemical washing. The system efficacy was further supported by a comparable phosphite removal efficiency of 72.8% in actual lake water conditions. Therefore, this TiO2/CeOx-CNT filter technology is promising for mitigating the challenging issue of phosphite contamination from water bodies.

19.
Artigo em Inglês | MEDLINE | ID: mdl-31776631

##### RESUMO

PURPOSE: 18F-FDG PET/CT has some limitations in the evaluation of multiple myeloma (MM). Since chemokine receptor-4 is overexpressed in MM, we perform a prospective cohort study to compare the performance of 68Ga-Pentixafor and 18F-FDG PET/CT in newly diagnosed MM. METHODS: Thirty patients with newly diagnosed MM were recruited. All patients underwent 68Ga-Pentixafor and18F-FDG PET/CT within 1 week after enrollment. A positive PET/CT was defined as the presence of focal PET-positive lesions in bone marrow or diffuse bone marrow patterns (uptake > liver). Bone marrow uptake values in 68Ga-Pentixafor and18F-FDG PET/CT (total bone marrow glycolysis [TBmGFDG], total bone marrow uptake with 68Ga-Pentixafor [TBmUCXCR4], total bone marrow volume [TBmV], SUVmean, and SUVmax) were obtained by drawing total bone marrow volume of interest on PET/CT. The positive rates of the PET/CT scans were statistically compared, and the correlation between quantitative bone marrow uptake values and clinical characteristics, laboratory findings, and staging was analyzed. RESULTS: 68Ga-Pentixafor PET/CT had a higher positive rate than 18F-FDG PET/CT in recruited patients (93.3 vs. 53.3%, p = 0.0005). In quantitative analysis, bone marrow uptake values in 68Ga-Pentixafor (TBmUCXCR4, SUVmax, and SUVmean) were positively correlated with end organ damage, staging, and laboratory biomarkers related to tumor burden including serum ß2-microglobulin, serum free light chain, and 24-h urine light chain (p < 0.05). In 18F-FDG PET/CT, only the SUVmean of total bone marrow was positively correlated with serum free light chain and 24-h urine light chain (p < 0.05). CONCLUSIONS: 68Ga-Pentixafor PET/CT is promising in assessment of newly diagnosed MM. TRIAL REGISTRATION NUMBER: NCT03436342.

20.
BMC Public Health ; 19(1): 1459, 2019 Nov 06.
Artigo em Inglês | MEDLINE | ID: mdl-31694592

##### RESUMO

BACKGROUND: Urbanization and aging are global phenomena that offer unique challenges in different countries. A supportive environment plays an important role in addressing the issues of health behavioral change and health promotion (e.g., prevent chronic illnesses, promote mental health) among older adults. With the development of the socio-ecological theoretical model, studies on the impact of supportive environments on physical activity have become popular in the public health field in the EU and US. Meanwhile, very few Chinese studies have examined the relationship between built environment features and older adults' physical activity at the ecological level. The purpose of the study is to investigate how the factors part of the built environment of Nanjing's communities also influence leisure time physical activity among the elderly. METHODS: Using a socio-ecological model as a theoretical framework, we conducted a cross-sectional study of 399 elderly people from 19 communities in Nanjing, China, using a one-on-one questionnaire to collect data, including participants' perceived built environment and self-reported physical activity. A multivariate linear regression method was used to analyze the factors influencing their recreational physical activity. RESULTS: This study found that compared to older people with low average monthly income, the recreational physical activity of the elderly with average monthly incomes between 1001 and 2000 ¥ (ß = 23.31, p < 0.001) and 2001 ¥ or more (ß = 21.15, p < 0.001) are significantly higher. After controlling for individual covariates, street connectivity (ß = 7.34, p = 0.030) and street pavement slope (ß = - 7.72, p = 0.020), we found that two out of ten built environment factors indicators influence their physical activity. The importance of each influencing factor ranked from highest to lowest are monthly average income, street pavement slope, and street connectivity. Other factors were not significantly related to recreational physical activity by the elderly. CONCLUSIONS: Older adults with a high income were more likely to participate in recreational physical activity than those with a low income. In order to positively impact physical activity in older adults and ultimately improve health, policymakers and urban planners need to ensure that street connectivity and street pavement slope are factored into the design and development of the urban environment.