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1.
Chem Commun (Camb) ; 56(78): 11645-11648, 2020 Oct 07.
Artigo em Inglês | MEDLINE | ID: mdl-33000783

RESUMO

Two-dimensional (2D) multilayered graphitic carbon nanosheets are prepared via a facile, green, and mild method of one-pot hydrothermal carbonization at a temperature below 300 °C. Copper with a 2D structure is formed in situ and serves as both a template and catalyst. The obtained multilayered carbon nanosheets exhibit well-defined shapes and a radius-to-thickness ratio as high as 104, with monolayer thickness as small as 2.86 nm.

2.
Sci Rep ; 10(1): 16127, 2020 Sep 30.
Artigo em Inglês | MEDLINE | ID: mdl-32999311

RESUMO

Foveated near-eye display is one of the most promising approaches to deliver immersive experience of mixed reality. However, it is challenged to conceive a compact optical system. Here, we introduce a method to use polarization optics via liquid crystal photonics to improve the foveated display performance. We demonstrate a benchtop prototype of this idea. We implement and combine two display modules for peripheral and foveal visions. A peripheral display consists of a polarization selective lens (PSL) module, a polarization selective diffuser (PSD), and a slanted projection system. An 80[Formula: see text] diagonal field of view is achieved by on-axis optical configuration of the PSL module and the PSD. A foveal holographic display is composed of a spatial light modulator (SLM), a volume grating lens, and a microelectromechanical system mirror possibly in combination with a switchable polarization selective grating module. The holographic reconstruction using the SLM enables accurate focus cue generation and high resolution above 30 cycles per degree within 15[Formula: see text] by 15[Formula: see text] field of view. We explore and discuss the liquid crystal photonics in the prototype that has a novel optical design using volume gratings with polarization selectivity.

3.
Oncogene ; 2020 Oct 02.
Artigo em Inglês | MEDLINE | ID: mdl-33009487

RESUMO

The mechanism underlying EZH2 overexpression in breast cancer and its involvement in tumorigenesis remain poorly understood. In this study, we developed an approach to systematically identify the trans-acting factors regulating the EZH2 expression, and identified more than 20 such factors. We revealed reciprocal regulation of early growth response 1 (EGR1) and EZH2: EGR1 activates the expression of EZH2, and EZH2 represses EGR1 expression. Using CRISPR-mediated genome/epigenome editing, we demonstrated that EHZ2 represses EGR1 expression through a silencer downstream of the EGR1 gene. Deletion of the EGR1 silencer resulted in reduced cell growth, invasion, tumorigenicity of breast cancer cells, and extensive changes in gene expression, such as upregulation of GADD45, DDIT3, and RND1; and downregulation of genes encoding cholesterol biosynthesis pathway enzymes. We hypothesize that EZH2/PRC2 acts as a "brake" for EGR1 expression by targeting the EGR1 silencer, and EZH2 overexpression dampens tumor-suppressive signals mediated by EGR1 to drive breast tumorigenesis.

4.
Epigenetics ; : 1-11, 2020 Oct 04.
Artigo em Inglês | MEDLINE | ID: mdl-33016200

RESUMO

DNA methylation at CpG dinucleotides is one of the most extensively studied epigenetic marks. With technological advancements, geneticists can profile DNA methylation with multiple reliable approaches. However, profiling platforms can differ substantially in the CpGs they assess, consequently hindering integrated analysis across platforms. Here, we present CpG impUtation Ensemble (CUE), which leverages multiple classical statistical and modern machine learning methods, to impute from the Illumina HumanMethylation450 (HM450) BeadChip to the Illumina HumanMethylationEPIC (HM850) BeadChip. Data were analysed from two population cohorts with methylation measured both by HM450 and HM850: the Extremely Low Gestational Age Newborns (ELGAN) study (n = 127, placenta) and the VA Boston Posttraumatic Stress Disorder (PTSD) genetics repository (n = 144, whole blood). Cross-validation results show that CUE achieves the lowest predicted root-mean-square error (RMSE) (0.026 in PTSD) and the highest accuracy (99.97% in PTSD) compared with five individual methods tested, including k-nearest-neighbours, logistic regression, penalized functional regression, random forest, and XGBoost. Finally, among all 339,033 HM850-only CpG sites shared between ELGAN and PTSD, CUE successfully imputed 289,604 (85.4%) sites, where success was defined as RMSE < 0.05 and accuracy >95% in PTSD. In summary, CUE is a valuable tool for imputing CpG methylation from the HM450 to HM850 platform.

5.
Rev Esp Enferm Dig ; 2020 Oct 15.
Artigo em Inglês | MEDLINE | ID: mdl-33054296

RESUMO

OBJECTIVE: this study aimed to investigate the expression and clinical significance of miR-21 and miR-210 in serum of patients with colorectal cancer (CRC). METHODS: the expression levels of serum miR-21 and miR-210 in 40 CRC patients (CRC group) and 20 healthy patients (control group) were measured by qRT-PCR. Correlation analysis was performed of the relationship between serum miR-21 and miR-210 levels with clinical characteristics, including gender, age, tumor location, tumor size, tumor stage, local invasion and TNM staging. The expression levels of miR-21 and miR-210 in the CRC group were separately measured before and after surgery. ROC analysis was performed to evaluate the diagnostic value of miR-21 and miR-210. RESULTS: serum miR-21 and miR-210 in the CRC group were much higher than those in the control group. Meanwhile, the levels of serum miR-21 and miR-210 were closely related to tumor size (p = 0.028, p = 0.047), lymphatic metastasis (p = 0.038, p = 0.028), TNM staging (p = 0.014, p = 0.047) and tumor stage (p = 0.014, p = 0.017), but independent of gender, age and tumor location. In addition, serum miR-21 and miR-210 in the CRC group (n = 18) after surgery were lower than those before surgery (p < 0.001). ROC curves showed that miR-21 (AUC = 0.863) and miR-210 (AUC = 0.818) both had diagnostic efficacy in CRC patients. CONCLUSION: miR-21 and miR-210 can be used as novel non-invasive biomarkers for CRC diagnosis and prognosis.

6.
Cell Biol Int ; 2020 Oct 15.
Artigo em Inglês | MEDLINE | ID: mdl-33058436

RESUMO

Osteosarcoma (OS) is one of the most frequently malignant bone tumor types. Traditional treatments of OS involve standard chemotherapy or combination with radiation before and after surgery. Cisplatin is one of the most effectively chemotherapeutic drugs used for treating osteosarcoma. However, patients with advanced tumor stages develop cisplatin resistance, leading to the major clinical challenge. In this study, we investigated the roles of miR-329-3p in cisplatin sensitivity of osteosarcoma cells. We found miR-329-3p was significantly downregulated in osteosarcoma tissues compared with normal bone tissues. Overexpression of miR-329-3p suppressed osteosarcoma cell proliferation. Moreover, we observed low-toxic cisplatin treatments suppressed miR-329-3p but higher concentrations of cisplatin induced miR-329-3p expression. In addition, miR-329-3p was significantly downregulated in cisplatin resistant Saos-2 cells which displayed elevated glucose metabolism. Overexpression of miR-329-3p significantly impaired glucose metabolism of Saos-2 cells. Bioinformatics analysis and luciferase assay consistently demonstrated the glycolysis enzyme, lactate dehydrogenase-A (LDHA) was a direct target of miR-329-3p in osteosarcoma cells. Rescue experiments revealed restoration of LDHA in miR-329-3p-overexpressed cisplatin resistant cells effectively recovered glucose metabolism, resulting in increased cisplatin resistance. This study demonstrates a miR-329-3p-LDHA-glucose metabolism-cisplatin resistance axis in osteosarcoma cells, providing a miRNAs-based therapeutic strategy against chemoresistant osteosarcoma. This article is protected by copyright. All rights reserved.

7.
Clin Nutr ; 2020 Oct 01.
Artigo em Inglês | MEDLINE | ID: mdl-33077274

RESUMO

OBJECTIVE: To evaluate the nutritional risk and therapy in severe and critical patients with COVID-19. METHODS: A total of 523 patients enrolled from four hospitals in Wuhan, China. The inclusion time was from January 2, 2020 to February 15. Clinical characteristics and laboratory values were obtained from electronic medical records, nursing records, and related examinations. RESULTS: Of these patients, 211 (40.3%) were admitted to the ICU and 115 deaths (22.0%). Patients admitted to the ICU had lower BMI and plasma protein levels. The median Nutrition risk in critically ill (NUTRIC) score of 211 patients in the ICU was 5 (4, 6) and Nutritional Risk Screening (NRS) score was 5 (3, 6). The ratio of parenteral nutrition (PN) therapy in non-survivors was greater than that in survivors, and the time to start nutrition therapy was later than that in survivors. The NUTRIC score can independently predict the risk of death in the hospital (OR = 1.197, 95%CI: 1.091-1.445, p = 0.006) and high NRS score patients have a higher risk of poor outcome in the ICU (OR = 1.880, 95%CI: 1.151-3.070, p = 0.012). After adjusted age and sex, for each standard deviation increase in BMI, the risk of in-hospital death was reduced by 13% (HR = 0.871, 95%CI: 0.795-0.955, p = 0.003), and the risk of ICU transfer was reduced by 7% (HR = 0.932, 95%CI:0.885-0.981, p = 0.007). The in-hospital survival time of patients with albumin level ≤35 g/L was significantly decreased (15.9 d, 95% CI: 13.7-16.3, vs 24.2 d, 95% CI: 22.3-29.7, p < 0.001). CONCLUSION: Severe and critical patients with COVID-19 have a high risk of malnutrition. Low BMI and protein levels were significantly associated with adverse events. Early nutritional risk screening and therapy for patients with COVID-19 are necessary.

8.
Chemosphere ; : 128578, 2020 Oct 09.
Artigo em Inglês | MEDLINE | ID: mdl-33066970

RESUMO

In this study, we conducted proof-of-concept research towards the simultaneous treatment of livestock wastewater and the generation of high-quality biodiesel, through microalgae technology. Both original (OPE) and anaerobically-digested (DPE) piggery effluents were investigated for the culture of the microalgae, Desmodesmus sp. EJ8-10. After 14 days' cultivation, the dry biomass from microalgae cultivated in OPE increased from an initial value of 0.01 g/L to 0.33-0.39 g/L, while those growing in DPE only achieved a final dried mass of 0.15-0.35 g/L, under similar initial ammonium nitrogen (NH4+-N) concentrations. The significantly higher microalgal biomass production achieved in the OPE medium may have been supported by the abundance of both macronutrient, such as phosphorus (P), and of micronutrients, such as trace elements, present in the OPE, which may not been present in similar quantities in the DPE. However, a higher lipid content was observed (19.4-28%) in microalgal cells from DPE cultures than those (18.7-22.3%) from OPE cultures. Moreover, the fatty acid compositions in the microalgae cultured in DPE contained high levels of monounsaturated fatty acids (MUFAs) and total C16-C18 acids, which would afford a superior potential for high-quality biodiesel production. The N/P ratio (15.4:1) in OPE was much closer to that indicated by previous studies to be the most suitable (16:1) for microalgae growth, when compared with that determined from the DPE culture medium. This may facilitate protein synthesis in the algal cells and induce a lower accumulation of lipids. Based on these findings, we proposed a new flowsheet for sustainable livestock waste management.

9.
Artigo em Inglês | MEDLINE | ID: mdl-33069661

RESUMO

The outbreak of Coronavirus Disease 2019 (COVID-19) has become a severe global acute respiratory pandemic around the world in just a few months with an increasing number of infections and deaths. COVID-19 is a highly contagious and fatal disease. Almost everyone in the population is susceptible, and the incubation period is 1-14 days, mostly 3-7 days. The clinical symptoms of the COVID-19 are fever, dry cough and fatigue. Some patients are accompanied by symptoms such as nasal congestion, runny nose, sore throat, myalgia and diarrhea. Severe patients could even develop acute respiratory distress syndrome, septic shocks, metabolic acidosis and multifunctional organ failure, etc. Due to the relatively closed environment of dental clinics and the unique nature of dental procedures, both dental personnel and patients are easy to get infection through currently known respiratory droplet transmission, aerosol transmission, close contact transmission and other ways, inducing mutual cross-infection. Dental practitioners are facing unprecedented challenges due to the high risk of exposure to droplets and aerosols from saliva and other body fluids during dental procedures. Based on our experience and relevant research, this article introduces the basic knowledge about COVID-19 and the corresponding protective measures for dental practitioners, includes the risk of infection during dental procedures, the precautions related to the patients, infection control measures during dental treatment in clinics, protection measures at different levels for dental practitioners, and emergency dental treatment for confirmed COVID-19. It is the responsibility of every dental practitioner to fully understand the characteristics of the new coronavirus and strictly implement the most appropriate protective measures to reduce and control the risk of cross infection in dental procedures.

10.
J Hazard Mater ; 404(Pt B): 124119, 2020 Sep 29.
Artigo em Inglês | MEDLINE | ID: mdl-33075625

RESUMO

This work presents an overview about the suppressant enhanced explosion parameter (SEEP) phenomenon in aluminum dust explosion moderation. The SEEP phenomenon can be attributed to either the flammable gas produced by decomposition of insufficient chemical suppressant so as to form an explosible hybrid mixture, or to the improvement in dust dispersibility caused by small amounts of thermal inhibitor so as to form better dispersed dust clouds. Aluminum (Al) and four particle sizes of alumina (Al2O3) were used to confirm a physically caused SEEP phenomenon by performing flame propagation experiments. Higher flame spread velocities (FSVs) in Al dust clouds were found in the presence of 5 or 10% <150 and <45-µm Al2O3 powder. Adding micro-sized Al2O3 disrupted inter-particle contact in combustible dusts, decreased inter-particle forces, and formed dust clouds with better dispersibility, thereby decreasing the effective particle size distribution (PSD) of Al dust. A strong thermal effect brought about by 2.5 µm Al2O3 overcame the negative effect of improved dispersion, preventing SEEP from occurring. The addition of 50 nm Al2O3 increased cohesion of powder mixtures, and decreased dust dispersibility. With benefits from both dispersion suppression and the thermal effect, Al flame propagation was well quenched.

11.
Artigo em Inglês | MEDLINE | ID: mdl-33044695

RESUMO

Arsenic (As) pollution can lead to an element imbalance in rice. A hydroponic study was carried out to examine the influence of inorganic (arsenate) and organic (dimethylarsinic acid (DMA)) arsenic compounds on the concentration and distribution of iron (Fe), manganese (Mn), copper (Cu), zinc (Zn), nickel (Ni), carbon (C), nitrogen (N), and sulfur (S) in rice caryopsis at maturity using laser confocal microscopy and synchrotron X-ray fluorescence (SXRF). Results showed that treatments with inorganic (iAs) and organic (DMA) arsenic did not change the distribution characteristics of the above elements in rice grains. Fe, Mn, and iAs were mainly limited to the ventral ovular vascular trace, while Cu, Zn, and DMA extended into the endosperm. This implies that milling processes are likely to remove a majority of Fe, Mn, and iAs, but not Cu, Zn, and DMA. With regard to the average fluorescent intensity of the rice endosperm, iAs exposure caused significant reductions in Mn (53%), Fe (40%), Cu (27%), and Zn (74%) while DMA treatments decreased Mn (49%), Fe (37%), and Zn (21%). Compared with DMA, iAs exerted more influence on the reduction of these elements in rice caryopsis. In addition, the elemental analysis revealed a significant 12.7% increase for N and 8% reduction for S in DMA-treated rice caryopsis while a significant decrease of 24.0% for S in iAs-exposed rice caryopsis. These findings suggest that Cu, Zn, and S are more easily impacted by iAs, while N is mostly affected by DMA.

12.
Lancet Oncol ; 21(10): 1378-1386, 2020 10.
Artigo em Inglês | MEDLINE | ID: mdl-33002439

RESUMO

BACKGROUND: Genetic variants and lifestyle factors have been associated with gastric cancer risk, but the extent to which an increased genetic risk can be offset by a healthy lifestyle remains unknown. We aimed to establish a genetic risk model for gastric cancer and assess the benefits of adhering to a healthy lifestyle in individuals with a high genetic risk. METHODS: In this meta-analysis and prospective cohort study, we first did a fixed-effects meta-analysis of the association between genetic variants and gastric cancer in six independent genome-wide association studies (GWAS) with a case-control study design. These GWAS comprised 21 168 Han Chinese individuals, of whom 10 254 had gastric cancer and 10 914 geographically matched controls did not. Using summary statistics from the meta-analysis, we constructed five polygenic risk scores in a range of thresholds (p=5 × 10-4 p=5 × 10-5 p=5 × 10-6 p=5 × 10-7, and p=5 × 10-8) for gastric cancer. We then applied these scores to an independent, prospective, nationwide cohort of 100 220 individuals from the China Kadoorie Biobank (CKB), with more than 10 years of follow-up. The relative and absolute risk of incident gastric cancer associated with healthy lifestyle factors (defined as not smoking, never consuming alcohol, the low consumption of preserved foods, and the frequent intake of fresh fruits and vegetables), was assessed and stratified by genetic risk (low [quintile 1 of the polygenic risk score], intermediate [quintile 2-4 of the polygenic risk score], and high [quintile 5 of the polygenic risk score]). Individuals with a favourable lifestyle were considered as those who adopted all four healthy lifestyle factors, those with an intermediate lifestyle adopted two or three factors, and those with an unfavourable lifestyle adopted none or one factor. FINDINGS: The polygenic risk score derived from 112 single-nucleotide polymorphisms (p<5 × 10-5) showed the strongest association with gastric cancer risk (p=7·56 × 10-10). When this polygenic risk score was applied to the CKB cohort, we found that there was a significant increase in the relative risk of incident gastric cancer across the quintiles of the polygenic risk score (ptrend<0·0001). Compared with individuals who had a low genetic risk, those with an intermediate genetic risk (hazard ratio [HR] 1·54 [95% CI 1·22-1·94], p=2·67 × 10-4) and a high genetic risk (2·08 [1·61-2·69], p<0·0001) had a greater risk of gastric cancer. A similar increase in the relative risk of incident gastric cancer was observed across the lifestyle categories (ptrend<0·0001), with a higher risk of gastric cancer in those with an unfavourable lifestyle than those with a favourable lifestyle (2·03 [1·46-2·83], p<0·0001). Participants with a high genetic risk and a favourable lifestyle had a lower risk of gastric cancer than those with a high genetic risk and an unfavourable lifestyle (0·53 [0·29-0·99], p=0·048), with an absolute risk reduction of 1·12% (95% CI 0·62-1·56). INTERPRETATION: Chinese individuals at an increased risk of incident gastric cancer could be identified by use of our newly developed polygenic risk score. Compared with individuals at a high genetic risk who adopt an unhealthy lifestyle, those who adopt a healthy lifestyle could substantially reduce their risk of incident gastric cancer. FUNDING: National Key R&D Program of China, National Natural Science Foundation of China, 333 High-Level Talents Cultivation Project of Jiangsu Province, and China Postdoctoral Science Foundation.


Assuntos
Predisposição Genética para Doença/genética , Estilo de Vida Saudável , Neoplasias Gástricas/genética , Adulto , Idoso , Grupo com Ancestrais do Continente Asiático , China/epidemiologia , Feminino , Seguimentos , Predisposição Genética para Doença/epidemiologia , Predisposição Genética para Doença/psicologia , Estudo de Associação Genômica Ampla/estatística & dados numéricos , Humanos , Incidência , Masculino , Pessoa de Meia-Idade , Herança Multifatorial , Polimorfismo de Nucleotídeo Único , Estudos Prospectivos , Fatores de Risco , Neoplasias Gástricas/epidemiologia , Neoplasias Gástricas/psicologia
13.
Zhongguo Zhong Yao Za Zhi ; 45(15): 3659-3665, 2020 Aug.
Artigo em Chinês | MEDLINE | ID: mdl-32893555

RESUMO

As a traditional Chinese medicinal material, Chrysosplenium is urgently needed for genetic resource investigation and protection research due to the decrease of its wild resources in recent years. After investigating the wild resources, we conducted genetic polymorphism and clustering studies of 24 species(a total of 36 samples) of Chrysosplenium using SRAP technique. The results showed that a total of 374 polymorphic bands were obtained using 18 pairs of SRAP primers to amplify these samples, on average of 20.7 bands for each primer pair. We used the biological software to analyze the population's genetic parameter and got the N_a value as 2.000 0, N_(e )value as 1.408 4, the average Nei's index as 0.263 5, and the average Shannon information index as 0.419 1. UPGMA cluster analysis showed that all the samples can be divided into three major groups at the genetic similarity coefficient of 0.70: there are 18 species(24 samples) gathered for the Ⅰ groups, 3 species or variation(7 samples) for Ⅱ groups, and 3 species(5 samples) for Ⅲ groups. The differences of these Chrysosplenium species at the molecular level are consistent with that of their geographical and ecological distribution. At the same time, we used SRAP technology to construct 36 DNA digital fingerprints of Chrysosplenium and obtained the unique molecular identification band type of each material. These results will provide effective methods and reliable basis for the identification, protection and genetic diversity analysis of the germplasm resources of Chrysosplenium, and lay a foundation for the further development and utilization of them.


Assuntos
Impressões Digitais de DNA , Variação Genética , Análise por Conglomerados , Marcadores Genéticos , Filogenia , Polimorfismo Genético
14.
Artigo em Inglês | MEDLINE | ID: mdl-32939914

RESUMO

Two-step-fabricated FAPbI3 -based perovskites have attracted increasing attention because of their excellent film quality and reproducibility. However, the underlying film formation mechanism remains mysterious. Here, the crystallization kinetics of a benchmark FAPbI3 -based perovskite film with sequential A-site doping of Cs+ and GA+ is revealed by in situ X-ray scattering and first-principles calculations. Incorporating Cs+ in the first step induces an alternative pathway from δ-CsPbI3 to perovskite α-phase, which is energetically more favorable than the conventional pathways from PbI2 . However, pinholes are formed due to the nonuniform nucleation with sparse δ-CsPbI3 crystals. Fortunately, incorporating GA+ in the second step can not only promote the phase transition from δ-CsPbI3 to the perovskite α-phase, but also eliminate pinholes via Ostwald ripening and enhanced grain boundary migration, thus boosting efficiencies of perovskite solar cells over 23%. This work demonstrates the unprecedented advantage of the two-step process over the one-step process, allowing a precise control of the perovskite crystallization kinetics by decoupling the crystal nucleation and growth process.

15.
Chin J Dent Res ; 23(3): 199-204, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-32974620

RESUMO

OBJECTIVE: To investigate the bilateral symmetry of double root canals, variation in root canal bifurcation and position of canal orifices in mandibular incisors in a Chinese population. METHODS: A total of 149 subjects with mandibular incisors with two canals were selected from 866 patients based on CBCT images and divided into three groups: group 1 (< 21 years), group 2 (21-40 years) and group 3 (> 40 years). The prevalence of bilateral symmetry of double root canals (type III and type V), the distance between the cementoenamel junction (CEJ) and the bifurcation (D1) and the distance between the two canal orifices (D2) were calculated and analysed. RESULTS: The bilateral symmetry of type III for mandibular central incisors (MCIs) (44.4%) was significantly lower than that for mandibular lateral incisors (MLIs) (63.4%). D1 was greater in group 1 (4.63 ± 1.35mm) than in group 2 (3.99 ± 1.02 mm) and group 3 (3.90 ± 1.95 mm). D2 was shorter in in MCIs (0.65 ± 0.20 mm) than in MLIs (0.74 ± 0.22 mm). CONCLUSION: Special attention is required in the root canal treatment of mandibular incisors, especially in patients aged above 21 years.


Assuntos
Cavidade Pulpar/diagnóstico por imagem , Incisivo , Adulto , Tomografia Computadorizada de Feixe Cônico , Humanos , Mandíbula/diagnóstico por imagem , Raiz Dentária , Adulto Jovem
16.
Sci Rep ; 10(1): 15567, 2020 Sep 23.
Artigo em Inglês | MEDLINE | ID: mdl-32968172

RESUMO

This study aimed to describe the landscape of Immune checkpoint inhibitors (ICIs)-related adverse events (AEs) in a predominantly Chinese cohort. We searched electronic datasets including PubMed, Web of Science and Embase to identify and recruit relevant trials up to September 2, 2019. Clinical trials focusing on ICIs in Chinese patients or a predominantly Chinese population were included. Incidences of treatment-related AEs (TRAEs) and immune-related AEs (irAEs) were pooled and compared. In total, we recruited 13 trials consisting of 1063 patients, with 922 (86.7%) receiving ICI monotherapy and 141 (13.3%) receiving combination of ICI with chemotherapy or anti-angiogenesis. The pooled incidence of any grade TRAEs, grade 1-2, grade 3-5 TRAEs, any grade irAEs, grade 1-2 irAEs and grade 3-5 irAEs in all 1063 patients were 84.1%, 63.3%, 20.9%, 43.3%, 40.0% and 3.0%, respectively. Moreover, 4.3% (44/1018) of patients experienced treatment discontinuation and only 8 (0.8%) patients experienced treatment-related death. Compared to ICI monotherapy, combination significantly increased grade 3-5 TRAEs (46.1% vs. 17.0%, P < 0.001) and grade 3-5 irAEs (7.1% vs. 2.0%, P = 0.015). By comparing the toxicity profiles between different ICIs, we found some drug-specific AEs such as reactive capillary haemangiomas for camrelizumab (58.6%), hyperglycemia for toripalimab (55.6%) and pyrexia for tislelizumab (54.3%). Additionally, nivolumab has the lowest incidence of any grade (64.1%) and grade 3-5 (11.8%) TRAEs. ICI-related AEs were generally mild and tolerable for a predominantly Chinese cohort. However, we should pay attention to the combination of ICI with chemotherapy as it could increase grade 3-5 TRAEs and irAEs.

18.
BMC Psychiatry ; 20(1): 477, 2020 Sep 29.
Artigo em Inglês | MEDLINE | ID: mdl-32993584

RESUMO

BACKGROUND: Individuals with major depressive disorder (MDD) have a high suicide risk. Some evidence suggests that uric acid (UA) may be involved in the pathophysiology of MDD. The purpose of this study was to evaluate whether serum UA levels were associated with suicide risk in MDD patients. METHODS: One hundred four female patients with MDD (52 patients with suicide risk and 52 patients without suicide risk) and 52 healthy individuals were included in this study. The suicide risk was evaluated by Mini International Neuropsychiatric Interview (M.I.N.I.). Fasting serum levels of UA, as well as glucose, lipid and renal function indicators were measured. RESULTS: Serum UA levels in MDD patients with suicide risk (245.01 ± 55.44 µmol/L) were significantly lower than those in MDD patients without suicide risk (274.17 ± 72.65 µmol/L) (p = 0.017) and healthy controls (271.42 ± 55.25 µmol/L) (p = 0.030). There was no difference in serum UA levels between the MDD patients without suicide risk and healthy controls (p = 0.821). Binary logistic regression analysis revealed a significant relationship between suicide risk and decreased serum UA levels (OR = 0.989, p = 0.010) in MDD patients. CONCLUSION: Decreased serum UA levels were associated with suicide risk in MDD patients. Purinergic system dysfunction may be involved in the neurobiological basis of suicide risk in these patients.

19.
Mol Cell Biochem ; 2020 Sep 04.
Artigo em Inglês | MEDLINE | ID: mdl-32888161

RESUMO

Acute myocardial infarction (AMI) can lead to myocardial injury, and long non-coding RNA (lncRNA) has been found to play an important regulatory role in the process of myocardial injury. However, the role and potential mechanisms of lncRNA testis-specific transcript Y-linked 15 (TTTY15) in AMI-induced myocardial injury has not been fully elucidated. Hydrogen peroxide (H2O2)-induced AMI cell model was built and AMI mice model were constructed. Relative expression levels of TTTY15, miR-98-5p and C-reactive protein (CRP) were determined by quantitative real-time PCR (qRT-PCR). Cell counting kit 8 (CCK8) assay, flow cytometry and enzyme-linked immunosorbent assay (ELISA) were employed to assess cell viability, apoptosis, inflammatory response and oxidative stress. Western blot (WB) analysis was used to assess the protein expression levels. The mechanism of TTTY15 was confirmed by dual-luciferase reporter assay and RNA immunoprecipitation (RIP) assay. Our results revealed that TTTY15 was upregulated and miR-98-5p was downregulated in AMI patients and H2O2-stimulated myocardial cells. Knockdown of TTTY15 could alleviate H2O2-stimulated myocardial cell injury in vitro and AMI progression in vivo. Bioinformatics analysis and the rescue experiments confirmed that TTTY15 positively regulated H2O2-induced myocardial cell injury via regulating CRP by sponging miR-98-5p. Our research proposed that lncRNA TTTY15 promoted myocardial cell injury by regulating the miR-98-5p/CRP axis, suggesting that TTTY15 might be a potential target for alleviating AMI-caused myocardial cell injury.

20.
Psychiatr Q ; 2020 Sep 03.
Artigo em Inglês | MEDLINE | ID: mdl-32885320

RESUMO

This study examined the prevalence of burnout and its association with quality of life (QOL) among psychiatric nurses in China.Ten psychiatric hospitals were included. Burnout and QOL were measured using standardized instruments. Altogether, 1449 nurses completed the assessment. The mean scores of emotional exhaustion (EE), depersonalization (DP) and personal accomplishment (PA) were 11.87 (SD = 6.72), 6.98 (SD = 5.71) and 22.06 (SD = 8.67), respectively. Of the participants, 59.8% (95% CI: 57-62%) experienced burnout; with 23.3% (95% CI: 21-25%) in EE, 14.6% (95% CI: 13-16%) in DP and 45.1% (95% CI: 43-48%) in PA. Psychiatric nurses who reported burnout had lower QOL in social (F (1, 1448) = 86.20, P < 0.001), physical (F (1, 1448) = 170.46, P < 0.001), psychological (F (1, 1448) = 205.63, P < 0.001), and environmental (F (1, 1448) = 120.24, P < 0.001) domains. Multiple logistic regression analysis revealed that alcohol users (P = 0.04; OR = 1.29, 95%CI: 1.01-1.64 in model 1 and P = 0.03; OR = 1.32, 95%CI: 1.04-1.69 in model 2) were significantly more susceptible to burnout, while senior nurses (P = 0.007; OR = 0.70, 95%CI: 0.53-0.91) and nurses with longer work experience (P = 0.02; OR = 0.70, 95%CI: 0.53-0.91) were less likely to develop burnout. Burnout is common in psychiatric nurses in China. In light of its negative impact on health and QOL, there is an urgent need for regular screening as well as effective preventive measures and interventions to reduce burnout within this at-risk occupational group.

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