Your browser doesn't support javascript.
Mostrar: 20 | 50 | 100
Resultados 1 - 20 de 3.480
Filtrar
1.
Protein Expr Purif ; 166: 105503, 2020 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-31550499

RESUMO

The N-glycosylation process that occurs in the Pichia pastoris protein expression system can have a significant effect on the yield of heterologous glycoproteins secreted from the yeast. The basis of the effect of N-glycosylation on yield, however, has not been elucidated. In order to investigate the effect of N-glycosylation on heterologous protein production, site-directed mutation was performed on five potential N-glycosylation sites of the tetanus toxin fragment C (TetC). Unaltered TetC (wild-TetC) and eight mutants, in which different numbers and locations of N-glycosylation sites were altered, were expressed in P. pastoris GS115. The recombinant target proteins presented different levels of N-glycosylation. The wild Tet-C and 4 mutations sites of putative N-glycosylation (4Gly mutant: N280Q) had the highest level of secreted protein, while 1 mutation of putative N-glycosylation sites (1Gly mutant: N39/64/85/205Q) had the highest level of intracellular, non-secreted heterologous protein. Reducing the number of native N-glycosylation sites decreased the level of glycosylation, as well as the level of secretion. Introduction of a N-glycosylation site at position 320, however, also reduced the level of expression and secretion of recombinant protein. These results indicate that the number and location of N-glycosylation sites jointly have an effect on the expression and secretion of heterologous glycoproteins in P. pastoris.

2.
Comput Methods Programs Biomed ; 183: 105073, 2020 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-31525548

RESUMO

BACKGROUND AND OBJECTIVE: Imaging genetics has been widely used to help diagnose and treat mental illness, e.g., schizophrenia, by combining magnetic resonance imaging of the brain and genomic information for comprehensive and systematic analysis. As a result, utilizing the correlation between magnetic resonance imaging of the brain and genomic information is becoming an important challenge. METHODS: In this paper, the joint analysis of single nucleotide polymorphisms and functional magnetic resonance imaging is conducted for comprehensive study of schizophrenia. We developed a deep canonically correlated sparse autoencoder to classify schizophrenia patients from healthy controls, which can address the limitation of many existing methods such as canonical correlation analysis, deep canonical correlation analysis and sparse autoencoder. RESULTS: The proposed deep canonically correlated sparse autoencoder can not only use complex nonlinear transformation and dimension reduction, but also achieve more accurate classifications. Our experiments showed the proposed method achieved an accuracy of 95.65% for SNP data sets and an accuracy of 80.53% for fMRI data sets. CONCLUSIONS: Experiments demonstrated higher accuracy of using the proposed method over other conventional models when classifying schizophrenia patients and healthy controls.

3.
Nurse Educ Today ; 84: 104205, 2020 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-31734641

RESUMO

BACKGROUND: Smoking is common among nursing students worldwide, but the reported prevalence is inconsistent across epidemiological studies. This is a meta-analysis of the prevalence of smoking in nursing students worldwide. DESIGN: Meta-analysis of observational studies. SAMPLE: A total of 46 studies were included in this meta-analysis. METHOD: Electronic databases (PubMed, Medline, PsycINFO, EMBASE and Web of science) were independently and systematically searched by two investigators from their commencement date up to 12 May 2018. Studies that reported the smoking rate of nursing students were included and analyzed using random-effects model. RESULTS: The pooled prevalence of current smoking was 26.6% (95% CI: 22.9-30.4%), while pooled prevalence of previous smoking was 15.5% (95% CI: 11.8-19.3%). Subgroup analyses showed that smoking rate was higher in male compared with female students (39% vs 25.2%, P < .001), while survey time, sample size, age, study design and academic year did not moderate the smoking rate (all P > .05). CONCLUSION: This meta-analysis confirmed that smoking is common in nursing students. Considering the negative impact of smoking on health, appropriate smoking cessation measures for nursing students should be developed.

4.
Behav Sleep Med ; : 1-14, 2019 Oct 31.
Artigo em Inglês | MEDLINE | ID: mdl-31672062

RESUMO

Objective: Poor sleep quality is common in nursing staff. This meta-analysis aimed to examine the pooled prevalence of poor sleep quality in nursing staff.Methods: A systematic search in PubMed, EMBASE, PsycINFO, and Web of Science databases was performed. Studies that reported sleep quality measured by the Pittsburgh Sleep Quality Index (PSQI) were synthesized using a random-effects model.Results: Fifty-three studies were analyzed. The pooled prevalence of poor sleep quality was 61.0% (95% CI: 55.8-66.1%). The pooled total PSQI score was 7.13 ± 0.18 (95% CI: 6.78-7.50). The pooled component scores were 1.47 ± 0.20 (95% CI of mean score: 1.08-1.85) in sleep latency, 0.91 ± 0.15 (95% CI of mean score: 0.61-1.21) in sleep duration, 1.59 ± 0.13 (95% CI of mean score: 1.35-1.84) in overall sleep disturbances, 0.33 ± 0.18 (95% CI of mean score: 0-0.67) in sleeping medication, 1.21 ± 1.20 (95% CI of mean score: 0.83-1.60) in daytime dysfunction, 1.39 ± 0.14 (95% CI of mean score: 1.11-1.67) in subjective sleep quality, and 0.66 ± 0.11 (95% CI of mean score: 0.44-0.87) in habitual sleep efficiency. Subgroup and meta-regression analyses found that PSQI cutoff values, mean age, body mass index (BMI), sample size, study quality, and work experience moderated the prevalence of poor sleep quality.Conclusions: Poor sleep quality appears to be common in nursing staff. Considering its negative impact on health, effective measures should be taken to improve poor sleep quality in this population. Longitudinal studies should be conducted to examine the contributing factors of nurses' poor sleep quality.

5.
Per Med ; 16(6): 449-455, 2019 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-31691635

RESUMO

Aim: Conotruncal heart defects (CTDs) are the most common form of congenital heart disease. We investigated the association of these two single-nucleotide polymorphisms (SNPs) in the promoter of miR-143/145 (rs353292 and rs4705343) with the susceptibility to CTDs in a Chinese population. Materials & methods: Two SNPs in the promoter of miR-143/145 (rs353292 and rs4705343) have been examined by PCR-RFLP methodology for 259 CTDs patients and 303 control subjects. Results: An association between SNP rs4705343 of miR-143/145 and CTDs has been confirmed in the Chinese Han population. Conclusion: Our results indicated that SNP rs4705343 in miR-143/145 is a potential genetic marker for CTDs in the Chinese Han population.

6.
Orthop Surg ; 2019 Nov 06.
Artigo em Inglês | MEDLINE | ID: mdl-31692295

RESUMO

OBJECTIVE: To use Gene Expression Omnibus (GEO) database coupled with Connectivity Map (CMap) databases to screen potential therapeutic drugs for osteonecrosis of femoral head (ONFH) rapidly. METHODS: Raw genetic data with accession number GSE74089 that contained eight hip articular cartilage specimens from four ONFH patients and four healthy controls were obtained from the Gene Expression Omnibus (GEO) database and were then integrated using R to identify differentially expressed genes (DEGs). Subsequently, to identify several potential small molecular compounds that were most strongly negatively correlated with ONFH, a search query of DEGs was explored by using CMap. RESULTS: Filtering revealed 1937 DEGs with log (fold-change) ≥1 and adjust P value < 0.001. Finally, a network of candidate targets for ONFH with 135 nodes and 660 edges was constructed through network topology analysis, including 96 up-regulated genes and 39 down-regulated genes. Several significant gene functions and signaling pathways associated with pathological processes of ONFH were identified via gene enrichment analysis. Based on the CMap database, some potential small molecular components that may be possible to counteract the effects of molecular signal imbalance for ONFH were identified. Neostigmine bromide with low CMap score and P value and specificity score was predicted to be the most candidate compound, involved in the "positive regulation of stem cell proliferation," "regulation of protein autophosphorylation," "VEGF signaling pathway," and "ECM-receptor interaction." CONCLUSIONS: The GEO and CMap databases can be effectively used in understanding the molecular changes in ONFH and provide a systematic manner to identify potential drugs for ONFH prevention and treatment. However, additional clinical and experimental research of the candidate compound is warranted.

7.
Clin Chim Acta ; 2019 Oct 31.
Artigo em Inglês | MEDLINE | ID: mdl-31678572

RESUMO

BACKGROUND: Osteopontin (OPN), a matricellular protein, is greatly generated from brain tissues after acute brain injury. We determine the relationship between plasma OPN concentrations and outcome after acute intracerebral hemorrhage (ICH) in a clinical setting. METHODS: In this prospective, observational study enrolling 162 ICH patients and 162 healthy controls, hemorrhagic severity was assessed using National Institutes of Health Stroke Scale (NIHSS) scores and hematoma volumes, a poor outcome was defined as modified Rankin Scale >2 at poststroke 90 days, and early neurologic deterioration (END) was defined as an increase of ≥4 points in the NIHSS score or death at 24 h from symptoms onset. RESULTS: Plasma OPN concentrations were significantly higher in patients than in controls (median value, 1287.6 vs. 405.7 pmol/l; P < 0.001). OPN concentrations were strongly correlated with admission NIHSS scores (r value = 0.520) and hematoma volumes (r value = 0.468). Areas under receiver operating characteristic curve for poor outcome and END were 0.811 and 0.753 respectively. Plasma OPN emerged as an independent predictor of functional outcome and END, with odds ratio values of 3.897 and 6.004 respectively. CONCLUSIONS: Plasma OPN could serve as a useful prognostic biomarker in ICH.

8.
Artigo em Inglês | MEDLINE | ID: mdl-31733067

RESUMO

BACKGROUND AND AIM: A large number of papers reporting the relationships between body mass index (BMI) and esophageal cancer (EC) risk have been published in the past few decades, however, these result are inconsistent. Therefore, we carried out meta-analyses to explore the relationships between BMI and risk of EC (including esophageal squamous cell carcinoma (ESCC) and esophageal adenocarcinoma (EADC)). METHODS: We used the Web of science, PubMed and Embase to identify all published/online articles before December 30th, 2018, which yielded 25 articles eligible for data extraction (including 16,561 cases and 11,954,161 controls), and then pooled the relative risks (RRs) and corresponding 95% confidence intervals (CIs) using random-effects model. RESULTS: Our study presented that underweight had statistically significant association with risk of EC (RR = 1.78, 95%CI = 1.48-2.14, p<0.001) and ESCC (RR = 1.57, 95%CI = 1.20-2.06, p = 0.001) when compared with normal weight. Interestingly, both overweight and obesity could increase risk of EADC (RR = 1.56, 95%CI = 1.42-1.71, p<0.001; RR = 2.34, 95%CI = 2.02-2.70, p<0.001) while decrease risk of ESCC (RR = 0.71, 95%CI = 0.60-0.84, p<0.001; RR = 0.63, 95%CI = 0.60-0.84, p = 0.002). Additionally, obesity could increase risk of EC (RR = 1.51, 95%CI = 1.21-1.89, p<0.001). CONCLUSION: This meta-analyses provides a comprehensive and updated epidemiological evidence to confirm the associations between BMI and EC risk. These findings have public health implications with respect to better control body weight, and then reduce the occurrence of EC (including ESCC and EADC).

9.
Alcohol ; 2019 Nov 14.
Artigo em Inglês | MEDLINE | ID: mdl-31734306

RESUMO

Cholesterol homeostasis has been proposed to be implicated in the development of addiction. However, the effects of ethanol on cholesterol homeostasis within the brain are not well understood. One of the most important regulators of cholesterol homeostasis is HMG-CoA reductase (HMG-CoAR), the rate-limiting enzyme of cholesterol biosynthesis. We examined the phosphorylation of HMG-CoAR and the other key regulator of lipid synthesis, acetyl-CoA carboxylase (ACC), following acute or chronic treatment with ethanol (0.5, 1, or 2 g/kg) in the rat prefrontal cortex. The phosphorylation of AMP-activated protein kinase (AMPK), which regulate the HMG-CoAR activity, and its well-known upstream regulators were also studied. The phosphorylation of HMG-CoAR and ACC were transiently increased by ethanol treatment only in animals previously treated chronically with ethanol. Acute administration to naïve animals did not induce the phosphorylation, regardless of dosage. Similarly, the phosphorylation of AMPK and the upstream regulators, LKB1 and CaMK4, were transiently increased only in chronically ethanol-treated animals. In naïve animals, a high dose (2 g/kg) of ethanol decreased phosphorylation. The phosphorylation of TAK1, another upstream kinase of AMPK was increased only from 30 min to 24 h after the chronic treatment with ethanol. Together, these results indicate that repeated exposure is required for the activating effect of ethanol on HMG-CoAR and ACC. This effect seems to be mediated by the AMPK system, and may contribute to the long-lasting neuroadaptation involved in the development of alcohol dependence.

10.
Int J Colorectal Dis ; 2019 Nov 22.
Artigo em Inglês | MEDLINE | ID: mdl-31758247

RESUMO

OBJECTIVE: The evaluation of GI-pill gastrointestinal electronic capsule for colonic transit test in patients with slow transit constipation (STC) was studied. MATERIALS AND METHODS: STC patients (n = 162) were randomly divided into experimental group (n = 84, orally taken GI-pill gastrointestinal electronic capsule and X-ray granule capsule) and control group (n = 78, orally taken X-ray granule capsule). Comparison of the time in colonic transit test between the two groups was conducted. The data of GI-pill gastrointestinal electronic capsule in vivo time, time of capsule passing through the colon, the number of high amplitude propagating contractions (HAPCs), and physiological response ratio were analyzed. RESULTS: There were no significant differences in the whole colonic transit test time, right colonic transit time, left colonic transit time, and rectosigmoid colonic transit time between experimental group and control group (p > 0.05). All patients had no abdominal pain, nausea, vomiting, black stool, difficulty in electronic capsule excretion, or any other discomfort during the test. CONCLUSION: GI-pill gastrointestinal electronic capsule can continuously evaluate the dynamic characteristics of digestive tract in STC patients and is consistent with X-ray granule capsule, which is meaningful to clinical application.

11.
Br J Clin Pharmacol ; 2019 Nov 23.
Artigo em Inglês | MEDLINE | ID: mdl-31758576

RESUMO

AIMS: Human genetic, tissue expression, proteomics, transcriptomics, and nonclinical studies implicate tumor necrosis factor α-like ligand 1A (TL1A) as a novel target in inflammatory bowel disease (IBD). PF-06480605, a fully human immunoglobulin G1 monoclonal antibody, targets TL1A. This first-in-human, Phase 1, dose-escalation study assessed safety, tolerability, pharmacokinetics, pharmacodynamics, and immunogenicity of intravenous (IV) and subcutaneous (SC) PF-06480605 in healthy subjects (NCT01989143). METHODS: Ninety-two subjects were randomized to single ascending doses (SAD): PF-06480605 1 mg, 3 mg, 10 mg, 30 mg, 100 mg, 300 mg, 600 mg, or 800 mg IV, or multiple ascending doses (MAD): PF-06480605 3x500 mg IV, or 3x30 mg, 3x100 mg, or 3x300 mg SC every 2 weeks for 3 doses, or placebo. Safety, tolerability, pharmacokinetics, immunogenicity profiles, and total TL1A, anti-drug antibody (ADA), and neutralizing antibody (NAb) levels, were assessed at pre-determined times. RESULTS: PF-06480605 SAD up to 800 mg IV and MAD up to 300 mg x3 SC and 500 mg x3 IV were well tolerated. Overall, there were 45 and 44 treatment-emergent adverse events in SAD and MAD cohorts, respectively, and no deaths or serious adverse events. PF-06480605 exposure generally increased dose-dependently. ADA and NAb levels did not impact safety, pharmacokinetics, or pharmacodynamics at higher doses. Target engagement was demonstrated through dose-dependent differences in serum total soluble TL1A concentrations for PF-06480605 vs placebo cohorts. CONCLUSIONS: PF-06480605 was generally well tolerated, and binding of soluble TL1A was maintained throughout the dose interval, supporting further study of PF-06480605 in patients with IBD and other inflammatory conditions.

12.
Artigo em Inglês | MEDLINE | ID: mdl-31718126

RESUMO

In this article, the inkjet printing technique is demonstrated for the stacking of reduced graphene oxide (RGO) and molybdenum trioxide (MoO3) nanosheets for flexible all-solid-state micro-supercapacitors. The ammonium molybdate tetrahydrate/graphene oxide ((NH4)6Mo7O24·4H2O/GO) aqueous inks are facilely printed on polymide (PI) film and transformed to RGO/MoO3 hybrids via thermal treatments at air atmosphere. The compound inks are water-based, inkjet-printable, and nontoxic for inkjet printing to form two-dimensional crystal materials. The physical properties of aqueous inks are optimized within a printable range characterized by the Ohnesorge number of 1 < Z < 14. The inkjet-printed symmetric micro-supercapacitors (MSCs) with poly(vinyl alcohol) (PVA)-H2SO4 gel electrolyte possess a wide voltage window of 0-0.8 V, excellent flexibility, a high volumetric specific capacitance of 22.5 F cm-3 at 0.044 A cm-3, as well as good cyclic stability due to the synergistic effect of RGO and MoO3. Furthermore, the inkjet-printed composite MSCs delivered a maximum energy density of 2 mWh cm-3 and a power density of 0.018 W cm-3, and the capacity retention rate of inkjet-printed MSCs is still retained 82% even after 10 000 charge-discharge cycles, indicating good electrochemical properties. Above all, the as-designed inkjet printing technique shows potential for flexible and wearable energy storage electronics.

13.
Chin Med J (Engl) ; 132(22): 2684-2689, 2019 Nov 20.
Artigo em Inglês | MEDLINE | ID: mdl-31725446

RESUMO

BACKGROUND: Due to the different treatments for low-volume metastatic prostate cancer (PCa) as well as high-volume ones, evaluation of bone metastatic status is clinically significant. In this study, we evaluated the correlation between pre-treatment plasma fibrinogen and the burden of bone metastasis in newly diagnosed PCa patients. METHODS: A single-center retrospective analysis, focusing on prostate biopsies of newly diagnosed PCa patients, was performed. A total of 261 patients were enrolled in this study in a 4-year period. All subjects were submitted to single-photon emission computerized tomography-computed tomography to confirm the status of bone metastasis and, if present, the number of metastatic lesions would then be calculated. Clinical information such as age, prostate-specific antigen (PSA), fibrinogen, clinical T stage, and Gleason score were collected. Patients were divided into three groups: (i) a non-metastatic group, (ii) a high volume disease (HVD) group (>3 metastases with at least one lesion outside the spine), and (iii) a low volume disease (LVD) group (metastatic patients excluding HVD ones). The main statistical methods included non-parametric Mann-Whitney test, Spearman correlation, receiver operating characteristic (ROC) curves, and logistic regression. RESULTS: Fibrinogen positively correlated with Gleason score (r = 0.180, P = 0.003), PSA levels (r = 0.216, P < 0.001), and number of metastatic lesions (r = 0.296, P < 0.001). Compared with the non-metastatic and LVD groups, the HVD group showed the highest PSA (104.98 ng/mL, median) and fibrinogen levels (3.39 g/L, median), as well as the largest proportion of Gleason score >7 (86.8%). Both univariate (odds ratio [OR] = 2.16, 95% confidential interval [CI]: 1.536-3.038, P < 0.001) and multivariate (OR = 1.726, 95% CI: 1.206-2.472, P = 0.003) logistic regressions showed that fibrinogen was independently associated with HVD. The ROC curve suggested that fibrinogen acts as a predictor of HVD patients, yielding a cut-off of 3.08 g/L, with a sensitivity of 0.684 and a specificity of 0.760 (area under the curve = 0.739, 95% CI: 0.644-0.833, P < 0.001). CONCLUSIONS: Pre-treatment plasma fibrinogen is positively associated with bone metastatic burden in PCa patients. Our results indicate that fibrinogen might be a potential predictor of HVD.

14.
BMC Plant Biol ; 19(1): 473, 2019 Nov 06.
Artigo em Inglês | MEDLINE | ID: mdl-31694537

RESUMO

BACKGROUND: Soil salinization and alkalization are among the major agricultural threats that affect crop productivity worldwide, which are increasing day by day with an alarming rate. In recent years, several halophytes have been investigated for their utilization in soil remediation and to decipher the mechanism of salt-tolerance in these high salt tolerant genetic repositories. Suaeda salsa is an annual halophytic herb in the family Amaranthaceae, displaying high salt and alkali-resistance and having nutritive value. However, the fundamental biological characteristics of this valuable plant remain to be elucidated until today. RESULTS: In this study, we observed the morphology and development of Suaeda salsa, including seed morphology, seed germination, plant morphology, and flower development. Using microscopy, we observed the male and female gametophyte developments of Suaeda salsa. Also, chromosome behaviour during the meiosis of male gametophyte was studied. Eventually, the genome size of Suaeda salsa was estimated through flow cytometry using Arabidopsis as reference. CONCLUSIONS: Our findings suggest that the male and female gametophyte developments of Suaeda salsa are similar to those of the model plant Arabidopsis, and the diploid Suaeda salsa contains nine pairs of chromosomes. The findings also indicate that the haploid genome of Suaeda salsa is approximately 437.5 MB. The observations and results discussed in this study will provide an insight into future research on Suaeda salsa.

15.
iScience ; 21: 375-390, 2019 Oct 24.
Artigo em Inglês | MEDLINE | ID: mdl-31704649

RESUMO

Epigenomic changes and stem cell deterioration are two hallmarks of aging. Accumulating evidence suggest that senescence of mesenchymal stromal cells (MSCs) perpetuates aging or age-related diseases. Here we report that two H3K9 demethylases, KDM3A and KDM4C, regulate heterochromatin reorganization via transcriptionally activating condensin components NCAPD2 and NCAPG2 during MSC senescence. Suppression of KDM3A or KDM4C by either genetic or biochemical approach leads to robust DNA damage response and aggravates cellular senescence, whereas overexpression of KDM3A/KDM4C or NCAPD2 promotes heterochromatin reorganization and blunts DNA damage response. Moreover, MSCs derived from Kdm3a-/- mice exhibit defective chromosome organization and exacerbated DNA damage response, which are associated with accelerated bone aging. Consistently, analysis of human bone marrow MSCs and transcriptome database reveals inverse correlation of KDM3A/KDM4C and/or NCAPD2/NCAPG2 with aging. Taken together, the present finding unveils that H3K9 demethylases function as a surveillance mechanism to restrain DNA damage accumulation in stem cells during aging.

16.
Int J Mol Sci ; 20(23)2019 Nov 22.
Artigo em Inglês | MEDLINE | ID: mdl-31766732

RESUMO

The plant-specific transcription factor gene family, YABBY, belongs to the subfamily of zinc finger protein superfamily and plays an essential regulatory role in lateral organ development. In this study, nine YABBY genes were identified in the pineapple genome. Seven of them were located on seven different chromosomes and the remaining two were located on scaffold 1235. Through protein structure prediction and protein multiple sequence alignment, we found that AcYABBY3, AcYABBY5 and AcYABBY7 lack a C2 structure in their N-terminal C2C2 zinc finger protein structure. Analysis of the cis-acting element indicated that all the seven pineapple YABBY genes contain multiple MYB and MYC elements. Further, the expression patterns analysis using the RNA-seq data of different pineapple tissues indicated that different AcYABBYs are preferentially expressed in various tissues. RT-qPCR showed that the expression of AcYABBY2, AcYABBY3, AcYABBY6 and AcYABBY7 were highly sensitive to abiotic stresses. Subcellular localization in pineapple protoplasts, tobacco leaves and Arabidopsis roots showed that all the seven pineapple YABBY proteins were nucleus localized. Overexpression of AcYABBY4 in Arabidopsis resulted in short root under NaCl treatment, indicating a negative regulatory role of AcYABBY4 in plant resistance to salt stress. This study provides valuable information for the classification of pineapple AcYABBY genes and established a basis for further research on the functions of AcYABBY proteins in plant development and environmental stress response.

17.
Methods Enzymol ; 628: 243-262, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31668232

RESUMO

Ongoing advances in chemical proteomic methods have facilitated detection and quantification of enzymatic activity, a highly informative parameter that is not captured in protein abundance measurements. However, some biological questions remain unanswered, since current gel- or LC-MS/MS-based detection methods suffer from limitations stemming from sample homogenization, signal-averaging, and an inherent bias toward abundant proteins. To address these shortcomings, we recently developed an activity-based proximity ligation (ADPL) platform to capture and quantify enzyme activity on the level of single cells, with high intra- and intercellular spatial resolution. In this chapter, we briefly discuss the rationale behind the ADPL platform, the design transition from the initial "sandwich-complex" workflow to the optimized, "direct conjugate" ADPL method, and conclude with detailed protocols for each. We also describe our novel use of the homo-bifunctional linker, disuccinimidyl suberate (DSS), to conjugate proteins and oligonucleotides, thus generating the necessary antibody-oligonucleotide recognition reagents for ADPL. Finally, we demonstrate the utility of ADPL to characterize enzyme activity from cytosol to nucleus, and specifically detect enzyme activity using "direct conjugate" ADPL.

18.
Appl Opt ; 58(28): 7836-7843, 2019 Oct 01.
Artigo em Inglês | MEDLINE | ID: mdl-31674467

RESUMO

The extraction of effective information in visible-near-infrared (VIS-NIR) spectroscopy is crucial and difficult for spectral analysis. In this research, an algorithm of wavelet feature extraction based on the Gaussian kernel function (GKF-WTEF) was developed to suppress the influence of external interference on VIS-NIR spectroscopy and improve the accuracy of quantitative analysis. This algorithm takes the root-mean-square error of the prediction set (RMSEP) of the model, which is established by partial least-squares regression, as the optimization criteria. First, the optimal type of wavelet function, the decomposition level, and the Gauss kernel function central frequency band are determined according to the RMSEP. Second, the Gauss kernel function bandwidth is determined by Newton's method. Then, the Hadamard product of the Gaussian kernel function and the wavelet coefficient is obtained. Finally, the wavelet coefficients after the Hadamard product can be reconstructed to obtain the spectral data after feature extraction. In order to verify the effectiveness of this algorithm, the difference in the optical parameters of the polyvinyl chloride material container was used as an external interference source. And the spectrum of Intra-lipid and India-ink mixed solution with different concentrations was collected therein. The volume fraction of India-ink in complex mixed solution was quantitatively analyzed by using the RMSEP and the average relative error of the prediction set as the evaluation criteria. The research results demonstrated that the Gaussian-wavelet transform feature extraction algorithm is an effective pretreatment method, it can satisfactorily suppress the influence of external interference on the spectrum, and it can improve the analytical accuracy of VIS-NIR spectroscopy.

19.
Artigo em Inglês | MEDLINE | ID: mdl-31727519

RESUMO

In recent years, spectral quantitative analysis for blood components has been a research hotspot in biomedical engineering. But researches have been limited to the application of high-sensitivity spectroscopy instruments and the complexity of blood components-the overlapping of absorption curves for many components is severe. This has led to the difficulty in achieving satisfactory results when using spectroscopy to quantify components in blood. In order to enhance the model robustness and improve the model performance, this paper proposed a sample set partitioning strategy based on multi-component spatial distance (SSP-MCSD). Different from the other sample set partitioning strategies, which only consider the uniformity of the concentration distribution of the target component, this strategy also concerns to the concentration distribution of non-target components. The concentration of the target component and non-target components are used to construct a multi-dimensional space, and the Euclidean Distance of sample points in this space is used as the criterion to partition the sample set. At the same time, the spectra collected in multi-modes are fused for increasing the amount of information. So as to enhance the model robustness and to improve the analysis accuracy of the target components. In order to verify the effectiveness of this strategy, the serum of 101 volunteers was analyzed. Taking total protein in serum as the non-target component, the regression model for bilirubin concentration was established by transmission spectra, fluorescence spectra, and the joint spectra after fusion of the above two spectra, respectively. The experimental results showed that the prediction accuracy of the model established by SSP-MCSD combined with multi-mode spectral fusion is obviously higher than that of other methods. It can effectively improve the analysis accuracy of blood components.

20.
Clin Oral Investig ; 2019 Nov 15.
Artigo em Inglês | MEDLINE | ID: mdl-31728734

RESUMO

OBJECTIVES: To establish one method that can be used to quantitatively evaluate the condyle positional changes with 3D images in postoperative mandibular prognathism patients. MATERIALS AND METHODS: This is a retrospective observational study. Twenty-one patients who underwent bilateral sagittal split ramus osteotomy (BSSRO) were scanned with cone beam computed tomography (CBCT) for temporomandibular joints (TMJs) at 1 week preoperatively (T0), 1 to 2 weeks (T1), 3 months (T2), 6 months (T3), and 12 months (T4) postoperatively. The data were then grouped into T0T1, T1T2, T2T3, T3T4 and T0T1, T0T2, T0T3, and T0T4. Semi-automatic registration was conducted, and the condyle positional changes were measured in segmented 3D models. Inter- and intra-observer variability and the repeatability of registration were analyzed with paired t test; the repeated measurement analysis of variance was used for analyzing the repeatability of the marked points; the consistency of segmentation was analyzed with nonparametric test of multiple paired samples (Friedman test) and the independent-sample t test was applied to comparing changes between different periods of time. Differences were considered to be statistically significant when P < 0.05. RESULTS: In T0T1 and T1T2, the condylar position was changed greatly. In T2T3, the mean condylar translations were less than 0.2 mm in all directions, the mean rotational changes of condyle were less than 0.2 mm; in the period of T3T4, the mean condylar translations in all directions were less than 0.02 mm. For series 2, the condyle translational changes in axial, coronal, and sagittal views were within 0.10 mm, and the rotation direction of condyle in all three views was the same within 1 year after operation. CONCLUSIONS: Fused three-dimensional images can be used to qualitatively and quantitatively evaluate condyle positional changes. The condylar position might be stable at 3 months postoperatively. The condyles of most of patients did not fully return to their preoperative position within 1 year after the operation. CLINICAL RELEVANCE: One method for fusing images has been established to detect the condylar positional changes. This method may be applied to estimate the bony changes of condyle, even bony changes in other part of dentomaxillofacial region. Meanwhile, the data of condyle positional changes from asymptomatic patients after the surgery within 1 year can be used as a reference for further exploration of the relationship between orthognathic surgery and the occurrence of osteoarthritis postoperatively in the future. KEY POINTS: • By fused 3D images, the change of condylar position after bilateral sagittal split ramus osteotomy can be observed intuitively. • For the patients with mandibular prognathism, the condylar position would be stable at 3 months postoperatively. • The condyles of most mandibular prognathism patients did not fully return to their preoperative position within 1 year after operation.

SELEÇÃO DE REFERÊNCIAS
DETALHE DA PESQUISA