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1.
Artigo em Inglês | MEDLINE | ID: mdl-36673979

RESUMO

Soil erosion is an important global environmental issue that severely affects regional ecological environment and socio-economic development. The Yellow River (YR) is China's second largest river and the fifth largest one worldwide. Its watershed is key to China's economic growth and environmental security. In this study, six impact factors, including rainfall erosivity (R), soil erosivity (K), slope length (L), slope steepness (S), cover management (C), and protective measures (P), were used. Based on the revised universal soil loss equation (RUSLE) model, and combined with a geographic information system (GIS), the temporal and spatial distribution of soil erosion (SE) in the YR from 2000 to 2020 was estimated. The patch-generating land use simulation (PLUS) model was used to simulate the land-use and land-cover change (LUCC) under two scenarios (natural development and ecological protection) in 2040; the RUSLE factor P was found to be associated with LUCC in 2040, and soil erosion in the Yellow River Basin (YRB) in 2040 under the two scenarios were predicted and evaluated. This method has great advantages in land-use simulation, but soil erosion is greatly affected by rainfall and slope, and it only focuses on the link between land-usage alteration and SE. Therefore, this method has certain limitations in assessing soil erosion by simulating and predicting land-use change. We found that there is generally slight soil erosivity in the YRB, with the most serious soil erosion occurring in 2000. Areas with serious SE are predominantly situated in the upper reaches (URs), followed by the middle reaches (MRs), and soil erosion is less severe in the lower reaches. Soil erosion in the YRB decreased 11.92% from 2000 to 2020; thus, soil erosion has gradually reduced in this area over time. Based on the GIS statistics, land-use change strongly influences SE, while an increase in woodland area has an important positive effect in reducing soil erosion. By predicting land-use changes in 2040, compared to the natural development scenario, woodland and grassland under the ecological protection scenario can be increased by 1978 km2 and 2407 km2, respectively. Soil erosion can be decreased by 6.24%, indicating the implementation of woodland and grassland protection will help reduce soil erosion. Policies such as forest protection and grassland restoration should be further developed and implemented on the MRs and URs of the YR. Our research results possess important trend-setting significance for soil erosion control protocols and ecological environmental protection in other large river basins worldwide.


Assuntos
Rios , Solo , Erosão do Solo , Modelos Teóricos , Monitoramento Ambiental/métodos , Conservação dos Recursos Naturais
2.
Int Immunopharmacol ; 114: 109429, 2022 Nov 30.
Artigo em Inglês | MEDLINE | ID: mdl-36459921

RESUMO

Chronic renal failure (CRF) refers to progressive renal damage caused by chronic kidney diseases (CKD). Dialysis therapy and kidney transplantation are the important treatment for CRF. However, due to the limitation of conditions, they cannot be widely utilized. At present, the treatment of renal failure is a worldwide problem in clinic. Therefore, in the current study, we investigated the potential therapeutic effects of neoxanthin on CFR-caused aging and fibrosis. In this work, the effects of neoxanthin on CRF were studied using experimental techniques such as biochemistry, immunohistochemistry and molecular biology. In vitro, neoxanthin alleviated the aging and oxidative damage of kidney cells. In vivo, we found that Neoxanthin could alleviate adenine-induced CRF. Neoxanthin also inhibited CRF-caused renal aging, fibrosis, oxidative stress and inflammation. These findings indicate that neoxanthin could delay the progression of CRF and alleviate CRF-induced aging and fibrosis. Collectively, we found that neoxanthin shows good potential to inhibit CRF-caused kidney aging and fibrosis, suggesting that neoxanthin may be used as a drug (or a functional food) for the treatment of CRF-related diseases.

3.
Front Endocrinol (Lausanne) ; 13: 982493, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-36482992

RESUMO

Aims: The global COVID-19 pandemic has required a drastic transformation of prenatal care services. Whether the reformulation of the antenatal care systems affects maternal and infant outcomes remains unknown. Particularly, women with gestational diabetes mellitus (GDM) are among those who bear the greatest brunt. Thus, this study aimed to evaluate the impact of COVID-19 lockdown during late pregnancy on maternal and infant outcomes in women stratified by the GDM status in China. Study design: The participants were women who experienced the COVID-19 lockdown during late pregnancy (3185 in the 2020 cohort) or not (2540 in the 2019 cohort) that were derived from the Beijing Birth Cohort Study. Maternal metabolic indicators, neonatal outcomes, and infant anthropometrics at 12 months of age were compared between the two cohorts, stratified by the GDM status. Results: Participants who experienced COVID-19 lockdown in late pregnancy showed lower gestational weight gain than those in the control cohort. Nevertheless, they displayed a worse metabolic profile. COVID-19 lockdown during pregnancy was associated with higher glycosylated hemoglobin (HbA1c) (ß= 0.11, 95% CI = 0.05-0.16, q-value = 0.002) and lower high density lipoprotein cholesterol level (HDL-C) level (ß=-0.09, 95% CI = -0.14 to -0.04, q-value = 0.004) in women with GDM, adjusted for potential confounders. In normoglycemic women, COVID-19 lockdown in late pregnancy was associated with higher fasting glucose level (ß= 0.10, 95% CI = 0.08-0.12, q-value <0.0001), lower HDL-C level (ß=-0.07, 95% CI = -0.08 to -0.04, q-value <0.0001), and increased risk of pregnancy-induced hypertension (adjusted OR=1.80, 95%CI=1.30-2.50, q-value=0.001). The fasting glucose level decreased less from early to late pregnancy in women who experienced COVID-19 lockdown than in the controls, regardless of the GDM status. The HDL-C has risen less with COVID-19 lockdown in the normoglycemic subgroup. In contrast, no significant differences regarding neonatal outcomes or infant weight were found between the two cohorts. Conclusion: Experiencing the COVID-19 lockdown in pregnancy was associated with worse maternal metabolic status but similar neonatal outcomes and infant weight.


Assuntos
COVID-19 , Diabetes Gestacional , Recém-Nascido , Feminino , Humanos , Gravidez , Lactente , Masculino , Diabetes Gestacional/epidemiologia , COVID-19/epidemiologia , Estudos de Coortes , Pandemias , Controle de Doenças Transmissíveis , Glucose
4.
PLoS One ; 17(12): e0279550, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-36574388

RESUMO

The zebrafish (Danio rerio) is widely used as a promising high-throughput model organism in neurobehavioral research. The mobility of zebrafish can be dissected into multiple behavior endpoints to assess its neurobehavioral performance. However, such facilities on the market are expensive and clumsy to be used in laboratories. Here, we designed a low-cost, automatic zebrafish behavior assay apparatus, barely without unintentional human operational errors. The data acquisition part, composed of Raspberry Pi and HQ Camera, automatically performs video recording and data storage. Then, the data processing process is also on the Raspberry Pi. Water droplets and inner wall reflection of multi-well cell culture plates (used for placing zebrafish) will affect the accuracy of object recognition. And during the rapid movement of zebrafish, the probability of zebrafish tracking loss increased significantly. Thus, ROI region and related thresholds were set, and the Kalman filter algorithm was performed to estimate the best position of zebrafish in each frame. In addition, all functions of this device are realized by the custom-written behavior analysis algorithm, which makes the optimization of the setup more efficient. Furthermore, this setup was also used to analyze the behavioral changes of zebrafish under different concentrations of alcohol exposure to verify the reliability and accuracy. The alcohol exposure induced an inverted U-shape dose-dependent behavior change in zebrafish, which was consistent with previous studies, showcasing that the data obtained from the setup proposed in this study are accurate and reliable. Finally, the setup was comprehensively assessed by evaluating the accuracy of zebrafish detection (precision, recall, F-score), and predicting alcohol concentration by XGBoost. In conclusion, this study provides a simple, and low-cost package for the determination of multiple behavioral parameters of zebrafish with high accuracy, which could be easily adapted for various other fields.


Assuntos
Comportamento Animal , Peixe-Zebra , Animais , Humanos , Reprodutibilidade dos Testes , Gravação em Vídeo , Algoritmos
5.
Phys Med Biol ; 68(1)2022 Dec 27.
Artigo em Inglês | MEDLINE | ID: mdl-36573436

RESUMO

Objective.Magnetic particle imaging (MPI) is a novel radiation-free medical imaging modality that can directly image superparamagnetic iron oxide tracers (SPIOs) with high sensitivity, temporal resolution, and good spatial resolution. The MPI reconstruction task can be formulated mathematically as a Fredholm integral problem, but the concrete inversion is not easily possible because of the particle dynamics or scanner issues. Measurement based system matrix inversion takes these factors into account, but prior measurement and calibration are time consuming.Approach.We proposed a direct imaging method based on the weighted sum of harmonic signals. The harmonic signals with spatial information are obtained by the short-time Fourier transform, and odd harmonic components are selected for recombination and then mapped to the sampling trajectory to image the concentration distribution of SPIOs. In addition, we adopt a normalized-weighted sum of harmonics to improve the resolution of the native image.Main results.The effectiveness of the proposed method is verified by simulation imaging experiments and our in-house scanner-based experiments. Quantitative evaluation results show that compared with traditional methods, the structural similarity improved by 48%, mean square error decreased by 88%, and signal-to-artifact ratio increased by 2.5 times.Significance.The proposed method can rapidly image the concentration distribution of nanoparticles without any prior calibration measurements and reduce the blur of MPI images without deconvolution, which has the potential to be implemented as a multi-patch imaging method in MPI.


Assuntos
Nanopartículas de Magnetita , Nanopartículas de Magnetita/química , Diagnóstico por Imagem/métodos , Imagens de Fantasmas , Fenômenos Magnéticos
6.
Artigo em Inglês | MEDLINE | ID: mdl-36498433

RESUMO

Food security is crucial to world peace. Economic development has posed a great threat to the protection of cultivated land. Considering 20 cities in the lower Yellow River (AALYR) as the study area, this study explored the spatial evolution of cultivated land pressure (CLP) and economic development from 1998 to 2018, revealing the spatiotemporal coupling characteristics of the CLP index and economic development. The main results are as follows: we discerned that CLP and economic development have an obvious spatiotemporal consistency during 1998-2018. The CLP showed a spatial pattern of overall stability, as well as local changes. Most prefecture-level cities experienced decreased significantly in CLP and improvements in food security. Overall, there were regional differences in the coupling relationships between CLP and economic development in the study area. The explanatory power of the proportion of secondary and tertiary industries were significantly higher than other driving factors. Therefore, while developing the economy rapidly, we should also protect cultivated land resources and improve the coordination level between them, which is essential to guarantee food security and a steady economic development.


Assuntos
Desenvolvimento Econômico , Rios , Cidades , Indústrias , China , Conservação dos Recursos Naturais
7.
Respir Res ; 23(1): 316, 2022 Nov 19.
Artigo em Inglês | MEDLINE | ID: mdl-36403040

RESUMO

BACKGROUND: Eosinophilic asthma is a common subtype of severe asthma with high morbidity and mortality. The cytokine IL-5 has been shown to be a key driver of the development and progression of disease. Although approved monoclonal antibodies (mAbs) targeting IL-5/IL-5R have shown good safety and efficacy, some patients have inadequate responses and frequent dosing results in medication nonadherence. RESULTS: We constructed a novel trivalent bispecific nanobody (Nb) consisting of 3 VHHs that bind to 2 different epitopes of IL-5 and 1 epitope of albumin derived from immunized phage display libraries. This trivalent IL-5-HSA Nb exhibited similar IL-5/IL-5R blocking activities to mepolizumab (Nucala), an approved targeting IL-5 mAb. Surprisingly, this trivalent Nb was 58 times more active than mepolizumab in inhibiting TF-1-cell proliferation. In primate studies, the trivalent IL-5-HSA Nb showed excellent pharmacokinetic properties, and peripheral blood eosinophil levels remained significantly suppressed for two months after a single dose. In addition, the trivalent IL-5-HSA Nb could be produced on a large scale in a P. pastoris X-33 yeast system with high purity and good thermal stability. CONCLUSIONS: These findings suggest that the trivalent bispecific IL-5-HSA Nb has the potential to be a next-generation therapeutic agent targeting IL-5 for the treatment of severe eosinophilic asthma.


Assuntos
Asma , Eosinofilia Pulmonar , Animais , Interleucina-5/metabolismo , Interleucina-5/uso terapêutico , Eosinofilia Pulmonar/tratamento farmacológico , Eosinofilia Pulmonar/metabolismo , Asma/metabolismo , Eosinófilos/metabolismo , Anticorpos Monoclonais/uso terapêutico
8.
Front Nutr ; 9: 1020281, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-36386955

RESUMO

The study aimed to investigate the effect of three kinds of natural antioxidants (NAs), such as curcumin, tea polyphenols (TP), and lycopene, on sweet potato starch's structure and physicochemical properties of starch noodles. We found that the broken rates, iodine blue values, hardness, and chewiness of natural antioxidant starch noodles (NASN) were increased with the addition of the NAs. Additionally, the elasticity decreased with the addition of curcumin and lycopene, but it increased with the addition of TP. The cross-section structure of NASN obtained by scanning electron microscope (SEM) showed more holes appeared when adding NAs, and the additional amount had a pronounced effect on the microstructure of starch noodles (SN) regardless of the kind of NA added. The X-ray diffraction detection showed that some crystal forms were significantly damaged, and the addition of NAs affected the crystallization process of starch and produced a small proportion of new crystals in the NASNs. The protective effects of SN on NAs and their antioxidant capacities under dry and room temperature storage (DRTS) and wet and frozen storage (WFS) conditions were optimal as compared to those of flour noodles (FN). The results showed that adding NAs could improve the sensory quality and antioxidant function of starch noodles. In turn, the dense structure of starch noodles can also have a significant protective effect on antioxidants and their antioxidant activities, which is especially obvious under WFS conditions.

9.
Artigo em Inglês | MEDLINE | ID: mdl-36418830

RESUMO

Saline-alkali stress is a serious abiotic factor which negatively impacts agricultural production and the ecological environment. Thus, improving the development of saline-alkali soil and reducing the effects of saline-alkali stress is a key issue for sustainable agricultural development and environmental protection. As such, it is unsurprising that researchers have lately focused on how to improve saline-alkali soil, increase the agricultural yield of saline-alkali land, and promote the adaptive growth of plants in saline-alkali soil. This paper reviews the latest research concerning nutrient content changes in saline-alkali soil, along with the associated changes in key nutrients in plants, to summarize which methods are most effective for improving the plant growth under saline-alkali stress. Finally, the prospects for alleviating saline-alkali stress and improving saline-alkali soil are put forward as a theoretical foundation for the stabilization of plant growth in saline-alkali soil, expansion of arable land area, crop yield improvement, and effective environmental protection.

10.
Cells ; 11(21)2022 Oct 24.
Artigo em Inglês | MEDLINE | ID: mdl-36359751

RESUMO

Coronavirus disease 2019 (COVID-19) is caused by the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), an infectious disease that has become a serious burden on global public health. This study screened and yielded specific nanobodies (Nbs) against SARS-CoV-2 spike protein receptor binding domain (RBD), following testing its basic characteristics. A nanobody phage library was established by immunizing a camel with RBD protein. After three rounds of panning, the positive colonies were screened by enzyme-linked immunosorbent assay (ELISA). By sequencing, four different sequences of nanobody gene fragments were selected. The four nanobody fusion proteins were expressed and purified, respectively. The specificity and affinity of the four nanobodies were identified by ELISA. Our results showed that an immune phage display library against SARS-CoV-2 has been successfully constructed with a library capacity of which was 4.7 × 108 CFU. The four purified nanobodies showed specific high-affinity binding SARS-CoV-2 S-RBD. Among these, the antigen binding affinity of Nb61 was more comparable to that of commercial rabbit anti-SARS-CoV-2 S-RBD antibodies. In sum, our study has obtained four nanobody strains against SARS-CoV-2 S-RBD with significant affinity and specificity, therefore laying an essential foundation for further research as well as the applications of diagnostic and therapeutic tools of SARS-CoV-2.


Assuntos
COVID-19 , Anticorpos de Domínio Único , Animais , Humanos , Coelhos , Glicoproteína da Espícula de Coronavírus/química , Anticorpos Neutralizantes , SARS-CoV-2 , Camelus
11.
Plants (Basel) ; 11(21)2022 Oct 25.
Artigo em Inglês | MEDLINE | ID: mdl-36365288

RESUMO

Salt stress could inhibit the growth and development of crops and negatively affect yield and quality. The objective of this study was to investigate the physiological responses of different asparagus cultivars to salt stress. Twenty days old seedlings ofasalt-tolerant Apollo andasalt-sensitive cultivar JL1 were subjected to 0 (CK) and120 mM NaCl stress for 20 d. Their changes in growth, ion contents, antioxidant enzyme activities and gene expression were analyzed. Salt stress significantly inhibited the growth of both cultivars, and JL1 showed a greater decrease than Apollo. The root development of Apollo was promoted by 120 mM NaCl treatment. The Na+ content in roots, stems, and leaves of both cultivars was increased under salt stress, while K+ content and K+/Na+ decreased. The salt-tolerant cultivar Apollo showed less extent of increase in Na+ and decrease in K+ content and kept a relatively high K+/Na+ ratio to compare with JL1. The contents of proline, soluble sugar and protein increased in Apollo, while thesesubstances changed differently in JL1 under salt stress. Activities of superoxide dismutase (SOD), peroxidase (POD), and catalase (CAT) were gradually increased under salt stress in Apollo, while the corresponding enzyme activities in JL1 were decreased at the late stage of salt stress. The expression of SOD, POD, and CAT genes of both cultivars changed in a similar way to the enzyme activities. Malondialdehyde (MDA) content was increased slightly in Apollo, while increased significantly in JL1. At the late stage of salt stress, Apollomaintained a relatively high K+/Na+, osmotic adjustment ability and antioxidant defense capability, and therefore exhibited higher tolerance to salt stress than that of JL1.

12.
BMC Genomics ; 23(1): 698, 2022 Oct 08.
Artigo em Inglês | MEDLINE | ID: mdl-36209069

RESUMO

BACKGROUND: The dried stem of Cistanche, is a famous Chinese traditional medicine. The main active pharmacodynamic components are phenylethanol glycosides (PhGs). Cistanche tubulosa produces higher level of PhGs in its stems than that of Cistanche deserticola. However, the key genes in the PhGs biosynthesis pathway is not clear in C. tubulosa. RESULTS: In this study, we performed the full-length transcriptome sequencing and gene expression profiling of C. tubulosa using PacBio combined with BGISEQ-500 RNA-seq technology. Totally, 237,772 unique transcripts were obtained, ranging from 199 bp to 31,857 bp. Among the unique transcripts, 188,135 (79.12%) transcripts were annotated. Interestingly, 1080 transcripts were annotated as 22 enzymes related to PhGs biosynthesis. We measured the content of echinacoside, acteoside and total PhGs at two development stages, and found that the content of PhGs was 46.74% of dry matter in young fleshy stem (YS1) and then decreased to 31.22% at the harvest stage (HS2). To compare with YS1, 13,631 genes were up-regulated, and 15,521 genes were down regulated in HS2. Many differentially expressed genes (DEGs) were identified to be involved in phenylpropanoid biosynthesis pathway, phenylalanine metabolism pathway, and tyrosine metabolism pathway. CONCLUSIONS: This is the first report of transcriptome study of C. tubulosa which provided the foundation for understanding of PhGs biosynthesis. Based on these results, we proposed a potential model for PhGs biosynthesis in C. tubulosa.


Assuntos
Cistanche , Álcool Feniletílico , Cistanche/genética , Cistanche/metabolismo , Perfilação da Expressão Gênica , Glicosídeos , Fenilalanina/metabolismo , Álcool Feniletílico/metabolismo , Tirosina/metabolismo
13.
Polymers (Basel) ; 14(19)2022 Sep 24.
Artigo em Inglês | MEDLINE | ID: mdl-36235949

RESUMO

In this study, in order to study the flexural behavior of fiber-reinforced polymer (FRP) bars with reinforced concrete beams under static loads after high-temperature exposure, seven pieces of FRP-reinforced concrete beams were subjected to static bending tests and calculation model derivations. Four-point bending tests were carried out on FRP-reinforced concrete beams after high temperature treatment. The effects of high temperature and types of FRP bars on the cracking load, crack development, deflection and ultimate capacity, and failure mode of concrete beams were investigated. The test results show that the maximum crack width, deflection, and ultimate bearing capacity of GFRP- and CFRP-reinforced concrete beams decrease obviously with a rise in high temperature. After the exposure of 400 °C for 2 h, compared with the behavior of concrete beams at room temperature, the maximum crack width of GFRP and CFRP-reinforced concrete beams increased by 42.9% and 41.7%, respectively, the deflection increases by 103.6% and 22.0%, and the ultimate bearing capacity decrease by 11.9% and 3.9%. Meanwhile, through the analysis of the existing research results and test results, the calculation models for the maximum crack width, deflection, and residual ultimate capacity of FRP-reinforced concrete beams after exposure of high temperature were proposed. For FRP-reinforced concrete beams after high-temperature exposure, the errors between the measured maximum crack width, stiffness, residual bearing capacity, and their corresponding calculated values using the model were mostly less than 10%. The calculated value using the proposed model in this research is in good agreement with the measured value. The mechanical properties of FRP-reinforced high-strength concrete structures after high-temperature exposure can be preliminarily predicted, which provides a new theoretical basis for the application of FRP-reinforced concrete structures.

14.
Front Endocrinol (Lausanne) ; 13: 1000296, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-36313758

RESUMO

Objective: Branched-chain amino acids (BCAAs) are closely associated with type 2 diabetes mellitus, but their roles in gestational diabetes mellitus (GDM) are still controversial. This study aims to explore the dynamic changes of BCAAs during pregnancy and identify potential early biomarkers for GDM. Methods: This study is a nested case-control study involved 49 women with GDM and 50 age- and body mass index (BMI)-matched healthy pregnant women. The dynamic changes of valine (Val), isoleucine (Ile), and leucine (Leu) were detected in the first (8-12 weeks) and second trimesters (24-28 weeks) by liquid chromatography-mass spectrometry. Results: Serum Val, Ile, and Leu were higher in GDM patients than in controls in the first trimester. Compared with the first trimester, the serum Val, Ile, and Leu in GDM patients were decreased in the second trimester. In addition, Val, Ile, and Leu in the first trimester were the risk factors for GDM, and Ile presented a high predictive value for GDM. Ile + age (≥ 35) + BMI (≥ 24) exhibited the highest predictive value for GDM (AUC = 0.902, sensitivity = 93.9%, specificity = 80%). Conclusion: Maternal serum Ile in the first trimester was a valuable biomarker for GDM. Ile combined with advanced maternal age and overweight may be used for the early prediction of GDM.


Assuntos
Diabetes Mellitus Tipo 2 , Diabetes Gestacional , Feminino , Gravidez , Humanos , Diabetes Gestacional/metabolismo , Aminoácidos de Cadeia Ramificada , Estudos de Casos e Controles , Segundo Trimestre da Gravidez , Biomarcadores
15.
Pain Physician ; 25(7): E1039-E1045, 2022 10.
Artigo em Inglês | MEDLINE | ID: mdl-36288589

RESUMO

BACKGROUND: Recurrent LDH (rLDH) is one of the most common causes of unsatisfactory outcomes after discectomy, which usually needs secondary surgery and leads to physical and psychological suffering for patients and substantial costs for society. OBJECTIVES: This study was conducted to analyze the risk factors of early rLDH (<= 6 months) and to reduce the incidence of early rLDH. STUDY DESIGN: A clinical retrospective study. METHODS: A total of 1,228 patients received percutaneous endoscopic lumbar discectomy surgery from January 2013 through December 2016; there was a minimum 5-year follow-up. Seventy-seven of them (6.27%) developed recurrences and were included in this study. According to the differences in recurrent time, patients were divided into 2 groups (<= 6 months and > 6 months). Clinical and radiological parameters were retrospectively collected through chart review and preoperative imaging. All related risk factors were collected and analyzed relative to the time of recurrent herniation. RESULTS: Patients with rLDH at <= 6 months and > 6 months were 49 and 28, respectively. Recurrence most often occurred within 6 months postoperatively, which was 63.6% of the total patients with rLDH. Of those risk factors, Modic changes, disc height index (DHI), and facet orientation (FO) showed significant statistical differences P = 0.003, P = 0.036, and P = 0.007, respectively). A logistic regression analysis was performed and showed there was an independent significant relationship between Modic changes (P = 0.042) and FO (P = 0.005) and early rLDH. LIMITATIONS: First, this was a retrospective nonrandomized study, and the number of patients with rLDH included in this study was relatively small. Second, limited risk factors were assessed in this study, and some relevant risk factors that were identified as significant independent predictors in other studies were not included in this study, such as canal diameter, annular defect size, migrated disc, and foraminoplasty. Third, this study compared the clinical and radiological parameters of patients with rLDH at different times, and one case-control study is needed for further study, especially in terms of standardized sampling and data classification. CONCLUSION: This study demonstrated that the recurrence rate of LDH at 5-year follow-up was 6.27% and there was a significant statistical relationship between FO, DHI, and Modic changes and early rLDH. Surgeons should take FO angles, DHI, and Modic change into consideration before surgery to achieve a satisfactory postoperative outcome and a relatively lower early recurrence rate. More patients and further investigation should be taken to assess the risk factors for early rLDH.


Assuntos
Discotomia Percutânea , Deslocamento do Disco Intervertebral , Humanos , Deslocamento do Disco Intervertebral/cirurgia , Deslocamento do Disco Intervertebral/epidemiologia , Estudos Retrospectivos , Discotomia Percutânea/métodos , Vértebras Lombares/cirurgia , Recidiva Local de Neoplasia/cirurgia , Discotomia/métodos , Fatores de Risco , Resultado do Tratamento
16.
Viruses ; 14(10)2022 09 26.
Artigo em Inglês | MEDLINE | ID: mdl-36298677

RESUMO

Hepatitis E virus (HEV) is an important public health burden worldwide, causing approximately 20 million infections and 70,000 deaths annually. The viral capsid protein is encoded by open reading frame 2 (ORF2) of the HEV genome. Most ORF2 protein present in body fluids is the glycosylated secreted form of the protein (ORF2S). A recent study suggested that ORF2S is not necessary for the HEV life cycle. A previously reported efficient HEV cell culture system can be used to understand the origin and life cycle of ORF2S but is not sufficient for functional research. A more rapid and productive method for yielding ORF2S could help to study its antigenicity and immunogenicity. In this study, the ORF2S (tPA) expression construct was designed as a candidate tool. A set of representative anti-HEV monoclonal antibodies was further used to map the functional antigenic sites in the candidates. ORF2S (tPA) was used to study antigenicity and immunogenicity. Indirect ELISA revealed that ORF2S (tPA) was not antigenically identical to HEV 239 antigen (p239). The ORF2S-specific antibodies were successfully induced in one-dose-vaccinated BALB/c mice. The ORF2S-specific antibody response was detected in plasma from HEV-infected patients. Recombinant ORF2S (tPA) can act as a decoy to against B cells. Altogether, our study presents a design strategy for ORF2S expression and indicates that ORF2S (tPA) can be used for functional and structural studies of the HEV life cycle.


Assuntos
Vírus da Hepatite E , Hepatite E , Camundongos , Animais , Vírus da Hepatite E/genética , Proteínas do Capsídeo/genética , Fases de Leitura Aberta , Camundongos Endogâmicos BALB C , Anticorpos Monoclonais/genética
17.
J Oncol ; 2022: 6051512, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-36299503

RESUMO

To explore the function and mechanism of lncRNA HOXA-AS2 in cancer-associated fibroblasts (CAFs)-derived exosomes in gallbladder cancer metastasis, and provide new research targets for the treatment of gallbladder cancer. At the same time, in order to clarify the early predictive value of lncRNA HOXA-AS2 for gallbladder cancer metastasis, and to provide a theoretical basis for clinical individualized treatment of gallbladder cancer. Methods. In our previous work, we used TCGA database analysis to find that lncRNA HOXA-AS2 was highly expressed in gallbladder cancer tissues compared with normal tissues. In this study, the expression levels of HOXA-AS2 in gallbladder cancer cell lines and control cells were first verified by QPCR and Western blot methods. Then, lentiviral tools were used to construct knockdown vectors (RNAi#1, RNAi#2) and negative control vectors targeting two different sites of HOXA-AS2, and the vectors were transfected into NOZ and OCUG-1 cells, respectively. Real-time PCR was used to detect knockdown efficiency. Then, the effects of silencing HOXA-AS2 on the proliferation, cell viability, cell migration, and invasion ability of gallbladder cancer cells were detected by MTT, plate cloning assay, Transwell migration chamber assay, and Transwell invasion chamber assay. Finally, the interaction between HOXA-AS2 and miR-6867 and the 3'UTR of YAP1 protein was detected by luciferase reporter gene. The results showed that the expression level of HOXA-AS2 in gallbladder cancer cell lines was higher than that in control cells. The expression of HOXA-AS2 in gallbladder carcinoma tissues was significantly higher than that in adjacent tissues (p < 0.05). After successful knockout of HOXA-AS2 by lentiviral transfection, the expression of HOXA-AS2 in gallbladder cancer cell lines was significantly decreased. Through cell proliferation and plate clone detection, it was found that silencing HOXA-AS2 inhibited cell proliferation and invasion. Through software prediction and fluorescein reporter gene detection, it was found that HOXA-AS2 has a binding site with miR-6867, and the two are negatively correlated, that is, the expression of miR-6867 is enhanced after the expression of HOXA-AS2 is downregulated. And the 3'UTR of YAP1 protein in the Hippo signaling pathway binds to miR-6867. Therefore, HOXA-AS2 may affect the expression of YAP1 protein by regulating miR-6867, thereby inhibiting the Hippo signaling pathway and promoting the proliferation and metastasis of gallbladder cancer cells. HOXA-AS2 is abnormally expressed in gallbladder cancer cells. HOXA-AS2 may promote the migration and invasion of gallbladder cancer cells by regulating the Hippo signaling pathway through miR-6867. HOXA-AS2 may serve as a potential diagnostic and therapeutic target for gallbladder cancer in clinic.

18.
Bioorg Chem ; 129: 106194, 2022 12.
Artigo em Inglês | MEDLINE | ID: mdl-36244321

RESUMO

Pancreatic islet transplantation is an ideal treatment strategy for type 1 diabetes mellitus (T1DM), but hypoxia-induced pancreatic ß cell death after islet transplantation is the huge obstacle that causes failure of this therapy. Thus, it become necessary to improve pancreatic ß cell viability under hypoxic conditions. In the present study, we designed mesenchymal stem cells (MSCs)-derived hypoxia-inducible factor 1α (HIF-1α)-overexpressed extracellular vesicle (EVs) (HIF-1α-EVs) and found that HIF-1α-EVs was effectively to promote cell viability and autophagy, and suppress cell apoptosis and senescence in the hypoxia-treated pancreatic ß cells. In addition, blockage of autophagy by its inhibitor 3-methyladenine (3-MA) abrogated the rescuing effects of HIF-1α-EVs on hypoxia-induced pancreatic ß cell death. Then, the potential underlying mechanisms by which HIF-1α-EVs triggered protective autophagy were uncovered, and we found that HIF-1α-EVs upregulated YTHDF1, resulting in the upregulation of autophagy-associated proteins (ATG5, ATG2A and ATG14), which were abrogated by deleting m6A writer METTL3. Finally, we verified that HIF-1α-EVs rescued cell viability, and reversed hypoxia-induced pancreatic ß cell apoptosis and senescence in a YTHDF1-dependent manner. Collectively, we concluded that MSCs-derived HIF-1α-EVs activated YTHDF1-mediated protective autophagy to promote pancreatic ß cell survival under hypoxic conditions, and HIF-1α-EVs could be used as candidate treatment strategy to increase the success rate of islet transplantation.


Assuntos
Vesículas Extracelulares , Células Secretoras de Insulina , Células-Tronco Mesenquimais , Humanos , Células Secretoras de Insulina/metabolismo , Hipóxia Celular , Autofagia , Apoptose , Vesículas Extracelulares/metabolismo , Células-Tronco Mesenquimais/metabolismo , Hipóxia/metabolismo , Metiltransferases/metabolismo , Proteínas de Ligação a RNA/metabolismo
19.
Genes (Basel) ; 13(9)2022 08 26.
Artigo em Inglês | MEDLINE | ID: mdl-36140703

RESUMO

Targeted combined immunotherapy has significantly improved the prognosis of patients with advanced hepatocellular carcinoma and has now become the primary treatment for advanced hepatocellular carcinoma. However, some patients still have poor efficacy or are resistant to treatment. The further exploration of molecular markers related to efficacy or finding molecular targets to increase efficacy is an urgent problem that needs to be resolved. In this research, we found that PROZ was a gene related to KDR expression that had significantly low expression in cancer tissue by analyzing the differential genes of cancer tissue and adjacent tissue and the intersection of KDR-related genes in hepatocellular carcinoma. The correlation analysis of clinical data showed that the low expression of PROZ was significantly correlated with the poor prognosis of hepatocellular carcinoma, and further studies found that PROZ was closely related to the expression of p-ERK and VEGFR2 in hepatocellular carcinoma. In addition, intracellular detection also showed that the expression of p-ERK increased and VEGFR2 expression decreased after PROZ interference, and PROZ downregulation with increased p-ERK and decreased VEGFR2 was also detected in sorafenib-resistant strains. At the same time, our analysis found that PROZ was negatively correlated with genes related to immunotherapy efficacy such as CD8A, CD274 and GZMA, and was also negatively correlated with T-cell infiltration in tumor tissue. Conclusion: PROZ is a gene related to the prognosis of hepatocellular carcinoma and it is closely related to the efficacy of sorafenib and immunotherapy. It may serve as a potential molecular target to improve the efficacy of targeted combined immunotherapy.


Assuntos
Carcinoma Hepatocelular , Neoplasias Hepáticas , Carcinoma Hepatocelular/tratamento farmacológico , Carcinoma Hepatocelular/genética , Humanos , Imunoterapia , Neoplasias Hepáticas/tratamento farmacológico , Neoplasias Hepáticas/genética , Prognóstico , Sorafenibe/farmacologia , Sorafenibe/uso terapêutico
20.
J Pain Res ; 15: 2867-2878, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-36124038

RESUMO

Purpose: To evaluate the outcomes, feasibility, and safety of endoscopic unilateral laminectomy, bilateral decompression and discectomy (Endo-ULBDD) for central lumbar spinal stenosis (CLSS) combined with disc herniation (DH). Methods: This study includes 39 patients diagnosed with CLSS combined with DH who met the inclusion criteria and underwent surgery for Endo-ULBDD from April 2020 to March 2021. The mean age of the patients, operation time, hospitalization time, time in bed, and complications were recorded. Patients were followed up for at least 12 months. Visual analog scale (VAS) scores for low-back and lower-limb pain and Oswestry Disability Index (ODI) scores were evaluated preoperatively, before discharge, and at 3, 6, and 12 months postoperatively. To evaluate clinical effectiveness 12 months postoperatively, the modified MacNab criteria were used. Results: The mean age of the patients was 59.9 years, the mean operation time was 82.1 minutes, the mean hospitalization time was 3.7 days, and the mean time in bed was 20.9 hours. The mean VAS scores of low-back and lower-limb pain improved from 5.9 and 7.2 to 2.0 and 1.6, respectively (P < 0.05). The ODI score improved from 56.0 to 16.7 (P < 0.05). The overall excellent-good rate of the modified MacNab criteria was 89.7%. Two kinds of complications occurred in 4 patients (10.3%), including 1 patient whose inferior articular process was excessively removed and 3 patients who suffered from postoperative dysesthesia. No other severe complications were noted. Conclusion: Endo-ULBDD is a safe, feasible, efficient, and minimally invasive approach to treating CLSS combined with DH.

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